CN108435379A - A kind of RNA milling and extractings system - Google Patents
A kind of RNA milling and extractings system Download PDFInfo
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- CN108435379A CN108435379A CN201810202666.3A CN201810202666A CN108435379A CN 108435379 A CN108435379 A CN 108435379A CN 201810202666 A CN201810202666 A CN 201810202666A CN 108435379 A CN108435379 A CN 108435379A
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- nitrogen
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- rna
- grinding head
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/08—Pestle and mortar
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
- B02C19/186—Use of cold or heat for disintegrating
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Food Science & Technology (AREA)
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- Immunology (AREA)
- Pathology (AREA)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to RNA extractive techniques fields, specifically a kind of RNA milling and extractings system, the RNA extracts grinding system and uses following RNA milling apparatus, including babinet, centrifuge tube, grinding head, power plant module, module, heat preservation module, nitrogen circulation pressurize module, nitrogen import modul and by die block are gripped, babinet is equipped with cantilever；Power plant module is fixed on cantilever；Grinding head is connect with power plant module, and power plant module drives grinding head positive and negative rotation, up and down linear motion；It grips module to be located on babinet, grips module for babinet to be fixed in centrifuge tube aid；Heat preservation module is used to keep the temperature centrifuge tube；Nitrogen circulation pressurize module is used for being passed through liquid nitrogen in babinet and nitrogen pressure in babinet being kept to stablize；Nitrogen import modul and babinet unicom, nitrogen import modul are used for importing nitrogen in grinding head；The present invention mechanically carries out RNA extraction grindings, can fully refine grinding, be conducive to the extraction of RNA.
Description
Technical field
The invention belongs to RNA extractive techniques field, specifically a kind of RNA milling and extractings system.
Background technology
RNA in cell can be divided into mRNA, transfer RNA and rRNA three categories, and purity is high, integrality is good
Total serum IgE is the prerequisite for carrying out the researchs such as gene cloning, gene expression, gene function analysis, this three classes RNA is present in carefully
In cytoplasm, therefore the essence of different tissues Total RNAs extraction is exactly to crack cell, releases RNA, and remove by different modes
The impurity such as albumen, DNA finally obtain the process of high pure rna product.
Traditional RNA sample is ground to hand lapping, and what is mostly used is that glass or ceramic grinding stick grind sample
Mill, but can make when hand lapping hand soreness and grinding it is insufficient, meanwhile, time-consuming for hand lapping sample, grind insufficient, this meeting
Influence RNA collects rate and quality.During grinding, due to liquid nitrogen highly volatile, needed in process of lapping constantly to
Liquid nitrogen is added in mortar, the consuming time is long, and inconvenient for operation, there are security risks.Also to keep temperature permanent in the vessel of grinding
It is fixed, prevent the RNA oxidizing ferment in air from being contacted with sample, sample overheats etc., and factors cause RNA to degrade.It is also kept away in process of lapping
The problems such as sample throughput caused by the splashing of unavoidable sample and liquid nitrogen is reduced and liquid nitrogen is to human skin frostbite.Again, liquid
Nitrogen low boiling point, highly volatile volatilize very fast in mortar, reduce the utilization rate of liquid nitrogen, so, urgent need will have mechanized equipment
System is come hand lapping mode when RNA extractions being replaced to grind, to eliminate some unnecessary defects when hand lapping.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of RNA milling and extractings system, present invention is mainly used for
Solve the problems, such as that hand lapping makes one hand soreness when tradition RNA extractions and hand lapping is offending.The present invention by babinet, grind
Bistrique, power plant module grip module, heat preservation module, nitrogen circulation pressurize module, nitrogen import modul and by die block
The mechanization that cooperating with each other realizes to sample fully refines grinding, and improves grinding efficiency, reduces sample and exists
The degree being degraded when grinding.
The technical solution adopted by the present invention to solve the technical problems is：A kind of RNA milling and extractings system, including babinet,
Centrifuge tube, grinding head, power plant module, grip module, heat preservation module, nitrogen circulation pressurize module, nitrogen import modul and
By die block, be provided on the babinet can up and down adjustment cantilever；The power plant module is fixed on cantilever；The grinding head
Connect with power plant module, power plant module drives grinding head positive and negative rotation, up and down linear motion, grinding head positive and negative rotation to sample into
Row agitation grinding, grinding head move up and down carries out crush and grind to sample, it is ensured that sample is ground finely；In the grinding head
Portion is hollow, and the big end of grinding head is equipped with the first venthole, discharges nitrogen for grinding head；The module that grips is located at babinet
On, module is gripped for babinet to be fixed in centrifuge tube aid；The heat preservation module is used to keep the temperature centrifuge tube；The nitrogen
Gas cycle pressurize module is used for being passed through liquid nitrogen in babinet and nitrogen pressure is kept in babinet to stablize, and ensure that liquid nitrogen and nitrogen
Normal supply；The nitrogen import modul and babinet unicom, the interior nitrogen evaporated of babinet enter nitrogen import modul, and nitrogen imports
Module is used to, to importing nitrogen in grinding head, make nitrogen overflow from the first venthole on grinding head, emerge bubble to interfere sample
Product are coagulated together completely, meanwhile, the RNA degrading enzymes in the nitrogen isolation air of spilling are contacted with sample；It is described to press die block
It is connect with power plant module, is used to make sample be not adhere in centrifugation inside pipe wall centrifuge tube slight amount by die block, to protect
Card sample can be all ground.It when work, adjusts cantilever and moves up, grip module aid centrifuge tube, adjust cantilever and move down,
So that grinding head is entered in sample and extend into centrifugation bottom of the tube；Nitrogen circulation pressurize module is opened, nitrogen circulation pressurize module is made
To being passed through nitrogen in babinet and nitrogen pressure in babinet being kept to stablize；Heat preservation module is opened, keeps the temperature in centrifuge tube constant, is dropped
The volatilization of low nitrogen keeps the activity of sample；Wait quietly to sample freezing and presenting solid-liquid admixture；It opens and presses die block, make
Centrifuge tube is pressed by die block, ensures that sample is non-condensing on centrifugation inside pipe wall；Nitrogen import modul is opened, is imported by nitrogen
Module makes nitrogen be overflowed from the first venthole on grinding head, the bubble emerged carrys out disturbed specimen to being passed through nitrogen in grinding head
It freezes, sample is made to condense into the graininess of 1mm-20mm, while the spilling of nitrogen, completely cut off RNA degrading enzymes and sample in air
Contact, reduces the degree that RNA is degraded；Cantilever and power plant module are adjusted, in centrifugation when grinding head being made to move to bottom
Bottom of the tube, and grinding head is kept not contacted with centrifugation bottom of the tube, conflict is moved down when grinding head being avoided to work to centrifugation bottom of the tube；It opens
Start power module, make power plant module drive grinding head, grinding head positive and negative rotation to form granular sample be stirred grinding,
Grinding head moves up and down carries out crush and grind to forming granular sample, until sample powdered needed for being ground into；It opens
Module is gripped, remaining each module is closed, takes out the centrifuge tube for filling required powdered samples, completes RNA extraction grindings.
The power plant module includes motor, cam, first axle, the first axle sleeve, thrust ball bearing, the second axis and the second axis
Set, the first axle are adapted with the first axle sleeve；The top of the first axle is equipped with pulley；Between the pulley and the first axle sleeve
Equipped with resetting spring；The motor driving cam rotation, cam are moved downward by pulley drive first axle, and resetting spring resets
First axle；The thrust ball bearing is between first axle and the second axis；The first axle lower end connects with thrust ball bearing upper end
It connects, the second axis upper end is connect with thrust ball bearing lower end；Realizing first axle using thrust ball bearing cannot rotate and the second axis
It can rotate；It is provided with the first spiral chute on the outer curved wall of second axis；Second sleeve lining is equipped with the first bulb,
And first bulb be adapted with the first spiral chute；Second axis is connect with the second axle sleeve rotational slide, the second axial lower fortune
It is dynamic, it realizes and generates positive rotation while the second axis moves downward, resetting spring resets first axle, drives in the second axial direction
Movement, realizes while the second axis moves upwards and generates reversed rotation, and the second axis only can be realized just with a motor
Reverse turn, up and down linear motion.Motor band moving cam rotates, and cam pushes first axle, resetting spring to reset and realizes in first axle
Lower movement, first axle drive the second axis to move up and down by thrust ball bearing, and the second axis matches realization second with the second axle sleeve
Axis keeps rotation while moving up and down.
The module that grips includes that aid pawl, wet cloth, condensate liquid and heating rod, the aid pawl are located at babinet upper end
And connect with babinet, aid pawl is for accommodating centrifuge tube；The wet cloth is located between aid pawl and centrifuge tube, and wet cloth edge prolongs
Heat preservation module is extended to, wet cloth has protective effect to centrifuge tube, avoids aid pawl from scratching centrifuge tube, while wet cloth has tentatively
Isolation sample contacted with the RNA degrading enzymes in outside air, reduce the possibility that RNA is degraded, meanwhile, reduce nitrogen
It overflows and is lost in, and there is sealing function to centrifuge tube and heat preservation module；The condensate liquid be located at centrifuge tube and heat preservation module it
Between, centrifuge tube and heat preservation module are fixed together by condensation liquid condensing icing, strengthen the fixed function to centrifuge tube；It is described to add
The caput of hot pin is located in condensate liquid, when needing to take out centrifuge tube after grinding, needs to melt the condensate liquid after solidifying, open
Opening heating rod and slightly being heated to condensate liquid makes condensate liquid melt, convenient for taking out centrifuge tube.It accommodates pawl and accommodates centrifuge tube, wet cloth lid
It avoids being scratched, while the isolation sample and the RNA in outside air that wet cloth is preliminary to centrifuging protection of pipe in centrifuge tube upper end
Degrading enzyme contacts, and wet cloth also there is sealing function, condensation liquid condensing to freeze centrifuge tube and heat preservation centrifuge tube and heat preservation module
Module is fixed together, and heating rod is opened after grinding and is slightly heated to condensate liquid makes condensate liquid melt, and is convenient for centrifuge tube
It takes out.
The heat preservation module includes heat preservation tank, and the second venthole, only logical plug and the first guiding are provided on the heat preservation tank
Hole, the only logical plug includes chock plug and stopper rod, and chock plug is linked together with stopper rod；The chock plug is located in the second venthole and chock plug
End stretch out the second venthole outside；The stopper rod is adapted with the first pilot hole；The first spring is arranged on the stopper rod；Institute
The first spring is stated between chock plug and the first pilot hole.When centrifuge tube to be put into heat preservation tank, the tube wall of centrifuge tube squeezes
Only lead to plug, only lead between plug and the second venthole and gap occur, the nitrogen in babinet will enter between insulating layer and centrifuge tube, add
Sample solidification in fast centrifuge tube；When taking out centrifuge tube, the first spring will only logical plug reset, and the second venthole is blocked.
The nitrogen circulation pressurize module includes the first nitrogen holding tank, the second nitrogen holding tank and liquid nitrogen bottle, and babinet is left
Side and right side are equipped with hole, the left side unicom of the first nitrogen holding tank and babinet；The second nitrogen holding tank and babinet
Right side unicom；It is connected with the first appendix between the first nitrogen holding tank and the second nitrogen holding tank；Described first is defeated
The first air valve and gasing pump are provided on tracheae；The liquid nitrogen bottle is connect with gasing pump, and liquid nitrogen bottle passes through the first appendix and defeated
Air pump is that babinet supplements liquid nitrogen；Gasing pump promotes circulating for the nitrogen in babinet；First air valve be located at gasing pump with
Between second nitrogen holding tank, the first air valve is used to open and close the circulation of nitrogen in the first appendix.The first air valve is opened,
So that liquid nitrogen bottle is passed through the first appendix and gasing pump and ensures that liquid nitrogen, nitrogen with normal supply and can follow to being passed through liquid nitrogen in babinet
Circulation is dynamic, it is ensured that sample can reduce sample setting time, improve grinding timeliness with rapid condensation at ice cube.
The nitrogen import modul includes guide sleeve and the second appendix, and one end and the babinet of second appendix join
Logical, the other end and the guide sleeve unicom of the second appendix, babinet provide source nitrogen by the second appendix for guide sleeve；Described
The second air valve is provided on two appendixs, the second air valve is used to open and close the circulation of nitrogen in the second appendix；The guide sleeve set
It is located on grinding head；The upper end of the grinding head is provided with third venthole and third venthole is located in guide sleeve.In babinet
By the second appendix into guide sleeve supplying nitrogen, for guide sleeve to being passed through nitrogen in grinding head, nitrogen in guide sleeve is logical
It crosses third venthole to enter in grinding head, nitrogen is overflowed by grinding head, bubble of emerging, and disturbed specimen freezes, and sample is made to condense
At the graininess of 1mm-20mm, be conducive to sample and be fully ground, reduce milling time, improves grinding efficiency；Meanwhile making nitrogen
Gas overflows, and the RNA degrading enzymes completely cut off in air are contacted with sample, and RNA is avoided to be degraded.
Described by die block includes drive shaft, pressing lever and interlocking lever, and the second spiral chute is provided in the drive shaft；
One end of the interlocking lever and pressing lever vertical connection, the other end of interlocking lever are provided with the second bulb；Second spiral slide
Slot is adapted with the second bulb, passes through drive shaft rotational movement pressing lever linear reciprocating motion；The drive shaft and power
It is driven by turbine and worm between module；The end of the pressing lever is provided with pressing plate flexible.Motor passes through turbine and worm
Drive shaft turns, drive shaft are driven to drive interlocking lever that the pressing plate on pressing lever is driven to press centrifuge tube so that when sample solidifies
Not with the solidification of centrifugation inside pipe wall, so that the sample that is ground into powder without being adhered on centrifugation inside pipe wall, when making ground sample,
Sample is all fully ground.
The beneficial effects of the invention are as follows：
1. a kind of RNA milling and extractings system of the present invention, the grinding head, power plant module, grip module, by
Die block be combined with each other, and with instead of grinding vessel, the grinding rod in milling tool in tradition, is ground, is disappeared by mechanization
In addition to the fatigue of underhand polish sample, frostbite of the liquid nitrogen splashing to human skin when avoiding hand lapping；Meanwhile power plant module
Grinding head is driven, grinding head positive and negative rotation is stirred grinding to sample, grinding head moves up and down carries out crush and grind to sample,
The grinding quality for improving sample makes sample be not adhere on centrifugation inside pipe wall centrifuge tube pressing by die block, it is ensured that
Sample can be all ground, and be combined with each other and allow sample by all fully fining grindings, improve collecting for RNA
Rate.
2. a kind of RNA milling and extractings system of the present invention, the babinet, heat preservation module, nitrogen import modul, nitrogen
Cycle pressurize module, nitrogen circulation pressurize module be combined with each other, and liquid nitrogen, nitrogen is made with normal supply and can to circulate,
The time of sample condensation is reduced, grinding timeliness is improved.
3. a kind of RNA milling and extractings system of the present invention, the heat preservation module, press die block at nitrogen import modul
Be combined with each other, it is ensured that sample not with centrifugation inside pipe wall condensation and sample freezing condensation after be graininess, be conducive to sample quilt
It is fully ground, reduces milling time, improve grinding efficiency；Meanwhile heat preservation module maintains temperature constant, reduces the volatilization of nitrogen,
Reduce the spilling of nitrogen；Nitrogen import modul continues to be passed through nitrogen to grinding head, and the nitrogen overflowed in grinding head has completely cut off sky
RNA degrading enzymes in gas are contacted with sample, reduce the degree that RNA is degraded.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the schematic diagram that the first axle of the present invention is connected with the second axis by bearing；
Fig. 3 is the second axis and the second axle sleeve connection diagram of the present invention；
Fig. 4 is the connection diagram of the guide sleeve and grinding head of the present invention；
Fig. 5 is the structural schematic diagram of the grinding head of the present invention；
Fig. 6 is A-A sectional views in Fig. 1；
In figure：Babinet 1, centrifuge tube 11, grinding head 12, cantilever 13, power plant module 2, motor 21, cam 22, first axle 23,
First axle sleeve 24, the second axis 26, the second axle sleeve 27, grips module 3, is aid pawl 31, wet cloth 32, cold thrust ball bearing 25
Lime set 33, heating rod 34, heat preservation module 4, heat preservation tank 41, the second venthole 42, only logical plug 43, nitrogen circulation pressurize module 5, the
One nitrogen holding tank 51, the second nitrogen holding tank 52, liquid nitrogen bottle 53, the first appendix 54, the first air valve 541, gasing pump 542,
Nitrogen import modul 6, guide sleeve 61, the second appendix 62, the second air valve 621, by die block 7, drive shaft 71, pressing lever 72,
Interlocking lever 73, pressing plate 721.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
According to shown in Fig. 1-6, a kind of RNA milling and extractings system, including babinet 1, centrifuge tube 11, grinding head 12, power mould
Block 2 grips module 3, heat preservation module 4, nitrogen circulation pressurize module 5, nitrogen import modul 6 and by die block 7, the case
Be provided on body 1 can up and down adjustment cantilever 13；The power plant module 2 is fixed on cantilever 13；The grinding head 12 and power
Module 2 connects, and power plant module 2 drives grinding head 12 positive and negative rotation, up and down linear motion, 12 positive and negative rotation of grinding head to sample into
Row agitation grinding, grinding head 12 move up and down carries out crush and grind to sample, it is ensured that sample is ground finely；The grinding head
The big end of 12 borings, grinding head 12 is equipped with the first venthole, discharges nitrogen for grinding head 12；It is described to grip module
3 are located on babinet 1, grip module 3 for babinet 1 to be fixed in the aid of centrifuge tube 11；The heat preservation module 4 be used for from
Heart pipe 11 is kept the temperature；The nitrogen circulation pressurize module 5 is used for being passed through liquid nitrogen in babinet 1 and keeping nitrogen pressure in babinet 1 steady
It is fixed, it ensure that the normal supply of liquid nitrogen and nitrogen；The nitrogen import modul 6 and 1 unicom of babinet, the interior nitrogen evaporated of babinet 1
Into nitrogen import modul 6, nitrogen import modul 6 is used to, to importing nitrogen in grinding head 12, make nitrogen from grinding head 12
First venthole overflows, and bubble of emerging carrys out disturbed specimen and is coagulated together completely, meanwhile, in the nitrogen isolation air of spilling
RNA degrading enzymes are contacted with sample；It is described to be connect with power plant module 2 by die block 7, it is used for by die block 7 slight to centrifuge tube 11
Pressing makes sample be not adhere on 11 inner wall of centrifuge tube, to ensure that sample can be all ground.When work, cantilever 13 is adjusted
Move up, grip module 3 accommodate centrifuge tube 11, adjust cantilever 13 move down, make grinding head 12 enter sample in and extend into from
11 bottom of heart pipe；Nitrogen circulation pressurize module 5 is opened, makes nitrogen circulation pressurize module 5 to being passed through nitrogen in babinet 1 and keeping case
Nitrogen pressure is stablized in body 1；Heat preservation module 4 is opened, keeps the temperature in centrifuge tube 11 constant, reduces the volatilization of nitrogen, keeps sample
The activity of product；Wait quietly to sample freezing and presenting solid-liquid admixture；It opens and presses die block 7, make to press centrifuge tube by die block 7
11, ensure that sample is non-condensing on 13 inner wall of centrifuge tube；Nitrogen import modul 6 is opened, by nitrogen import modul 6 to grinding head
It is passed through nitrogen in 12, nitrogen is made to be overflowed from the first venthole on grinding head 12, the bubble emerged carrys out disturbed specimen icing, makes sample
Product condense into the graininess of 1mm-20mm, while the spilling of nitrogen, and the RNA degrading enzymes completely cut off in air are contacted with sample, reduce
The degree that RNA is degraded；Cantilever 13 and power plant module 2 are adjusted, centrifuge tube 11 is in when grinding head 12 being made to move to bottom
Bottom, and grinding head 12 is kept not contacted with 11 bottom of centrifuge tube, conflict is moved down when grinding head 12 being avoided to work arrives centrifuge tube 11
Bottom；The module that turns on the power 2 makes power plant module 2 drive grinding head 12, and 12 positive and negative rotation of grinding head is to forming granular sample
It is stirred grinding, grinding head 12 moves up and down to forming granular sample progress crush and grind, until being ground into required powder
The sample of last shape；Opening grips module 3, closes remaining each module, takes out the centrifuge tube 11 for filling required powdered samples,
Complete RNA extraction grindings.
The power plant module 2 includes motor 21, cam 22, first axle 23, the first axle sleeve 24, thrust ball bearing 25, the
Two axis 26 and the second axle sleeve 27, the first axle 23 are adapted with the first axle sleeve 24；The top of the first axle 23 is equipped with pulley；
Resetting spring is equipped between the pulley and the first axle sleeve 24；21 driving cam 22 of the motor rotates, and cam 22 passes through pulley
Driving first axle 23 moves downward, and resetting spring resets first axle 23；The thrust ball bearing 25 is located at first axle 23 and second
Between axis 26；23 lower end of the first axle is connect with 25 upper end of thrust ball bearing, under 26 upper end of the second axis and thrust ball bearing 25
End connection；Realize that first axle 23 cannot rotate using thrust ball bearing 25 and the second axis 26 can rotate；Second axis 26
The first spiral chute is provided on outer curved wall；Second axle sleeve, 27 inner wall is equipped with the first bulb, and the first bulb and first
Spiral chute is adapted；Second axis 26 is connect with 27 rotational slide of the second axle sleeve, and the second axis 26 moves downward, and realizes
Positive rotation is generated while two axis 26 move downward, resetting spring resets first axle 23, and the second axis 26 is driven to move upwards,
It realizes while the second axis 26 moves upwards and generates reversed rotation, the second axis 26 only can be realized just with a motor 21
Reverse turn, up and down linear motion.Motor 21 is rotated with moving cam 22, and cam 22 pushes first axle 23, resetting spring to reset and realizes
First axle 23 moves up and down, and first axle 23 drives the second axis 26 to move up and down by thrust ball bearing 25, the second axis 26 and second
Axle sleeve 24, which matches, realizes that the second axis 26 keeps rotation while moving up and down.
The module 3 that grips includes aid pawl 31, wet cloth 32, condensate liquid 33 and heating rod 34, the aid pawl 31
It connect positioned at 1 upper end of babinet and with babinet 1, aid pawl 31 is for accommodating centrifuge tube 11；The wet cloth 32 be located at aid pawl 31 with
Between centrifuge tube 11, and 32 edge of wet cloth extends to heat preservation module 4, and wet cloth 32 has protective effect to centrifuge tube 11, avoids adding
It holds pawl 31 to scratch centrifuge tube 11, while there is wet cloth 32 preliminary isolation sample to contact with the RNA degrading enzymes in outside air,
The possibility that RNA is degraded is reduced, meanwhile, the spilling for reducing nitrogen is lost in, and is had to centrifuge tube 11 and heat preservation module 4
Sealing function；The condensate liquid 33 between centrifuge tube 11 and heat preservation module 4, condensate liquid 33 condensation freeze by centrifuge tube 11 with
Heat preservation module 4 is fixed together, and strengthens the fixed function to centrifuge tube 11；The caput of the heating rod 34 is located at condensate liquid 33
In, it when needing to take out centrifuge tube 11 after grinding, needs to melt the condensate liquid 33 after solidifying, opens heating rod 34 to condensation
Liquid 33, which slightly heats, makes condensate liquid 33 melt, convenient for taking out centrifuge tube 11.It accommodates pawl 31 and accommodates centrifuge tube 11, wet cloth 32 covers
11 upper end of centrifuge tube protects centrifuge tube 11, avoids being scratched, while in the isolation sample and outside air of wet cloth 32 tentatively
RNA degrading enzymes contact, and wet cloth 32 also has a sealing function to centrifuge tube 11 and heat preservation module 4, and the condensation of condensate liquid 33 freezes will be from
Heart pipe 11 is fixed together with heat preservation module 4, and heating rod 34 is opened after grinding and is slightly heated to condensate liquid 33 makes condensate liquid
33 melt, convenient for taking out centrifuge tube 11.
The heat preservation module 4 includes heat preservation tank 41, and the second venthole 42, only 43 and of logical plug are provided on the heat preservation tank 41
First pilot hole, the only logical plug 43 includes chock plug and stopper rod, and chock plug is linked together with stopper rod；The chock plug is located at the second ventilation
In the hole 42 and end of chock plug is stretched out outside the second venthole 42；The stopper rod is adapted with the first pilot hole；It is covered on the stopper rod
Equipped with the first spring；First spring is between chock plug and the first pilot hole.It is put into heat preservation tank 41 by centrifuge tube 11
When, the tube wall of centrifuge tube 11 squeezes only logical plug 43, only leads between plug 43 and the second venthole 42 and gap, the nitrogen in babinet 1 occurs
Gas will enter between insulating layer and centrifuge tube 11, accelerate the sample solidification in centrifuge tube 11；When taking out centrifuge tube 11, the first spring
It will only logical plug 43 reset, the second venthole 42 is blocked.
The nitrogen circulation pressurize module 5 includes the first nitrogen holding tank 51, the second nitrogen holding tank 52 and liquid nitrogen bottle 53,
1 left and right side of babinet is equipped with hole, the left side unicom of the first nitrogen holding tank 51 and babinet 1；Second nitrogen is temporary
Deposit the right side unicom of case 52 and babinet 1；It is connected with first between the first nitrogen holding tank 51 and the second nitrogen holding tank 52
Appendix 54；The first air valve 541 and gasing pump 542 are provided on first appendix 54；The liquid nitrogen bottle 53 and gasing pump
542 connections, liquid nitrogen bottle 53 are that babinet 1 supplements liquid nitrogen by the first appendix 54 and gasing pump 542；Gasing pump 542 promotes babinet 1
Interior nitrogen circulates；First air valve 541 is located between gasing pump 542 and the second nitrogen holding tank 52, the first gas
Valve 541 is used to open and close the circulation of nitrogen in the first appendix 54.The first air valve 541 is opened, makes liquid nitrogen bottle 53 by
One appendix 54 and gasing pump 542 ensure that liquid nitrogen, nitrogen with normal supply and can circulate to being passed through liquid nitrogen in babinet 1,
Ensure that sample can reduce sample setting time, improve grinding timeliness with rapid condensation at ice cube.
The nitrogen import modul 6 include guide sleeve 61 and the second appendix 62, one end of second appendix 62 with
1 unicom of babinet, the other end and 61 unicom of guide sleeve of the second appendix 62, babinet 1 are guide sleeve 61 by the second appendix 62
Source nitrogen is provided；The second air valve 621 is provided on second appendix 62, the second air valve 621 is for opening and closing the second appendix
The circulation of nitrogen in 62；The guide sleeve 61 is set on grinding head 12；The upper end of the grinding head 12 is provided with third ventilation
Hole and third venthole is located in guide sleeve 61.In babinet 1 by the second appendix 62 into guide sleeve 61 supplying nitrogen, lead
Air-casing 61 is to being passed through nitrogen in grinding head 12, the nitrogen in guide sleeve 61 is entered by third venthole in grinding head 12, nitrogen
It is overflowed by grinding head 12, bubble of emerging, disturbed specimen freezes, and so that sample is condensed into the graininess of 1mm-20mm, is conducive to sample
Product are fully ground, and milling time is reduced, and improve grinding efficiency；Meanwhile nitrogen being made to overflow, completely cut off the RNA degrading enzymes in air
It is contacted with sample, RNA is avoided to be degraded.
It is described to include drive shaft 71, pressing lever 72 and interlocking lever 73 by die block 7, it is provided with second in the drive shaft 71
Spiral chute；One end of the interlocking lever 73 and 72 vertical connection of pressing lever, the other end of interlocking lever 73 are provided with the second bulb；
Second spiral chute is adapted with the second bulb, is transported by 71 rotational movement pressing lever of drive shaft, 72 reciprocating linear
It is dynamic；It is driven by turbine and worm between the drive shaft 71 and power plant module 2；The end of the pressing lever 72 is provided with flexible
Pressing plate 721.Motor 21 drives drive shaft 71 to rotate by turbine and worm, and drive shaft 71 drives interlocking lever 73 to drive pressing lever 72
On pressing plate 721 press centrifuge tube 11 so that not with the solidification of 11 inner wall of centrifuge tube, so that be ground into powder when sample solidifies
Sample without being adhered on 11 inner wall of centrifuge tube, when making ground sample, sample is all fully ground.
When work, aid pawl 31 is made to accommodate centrifuge tube 11；Cantilever 13 moves down, grinding head 12 enter sample in and extend into from
11 bottom of heart pipe；The first air valve 541 is opened, liquid nitrogen bottle 53 is by the first appendix 54 and gasing pump 542 to being passed through liquid in babinet 1
Nitrogen simultaneously keeps nitrogen pressure in babinet 1 to stablize；Heat preservation tank 41 keeps the temperature in centrifuge tube 11 constant, reduces the volatilization of nitrogen,
Keep the activity of sample；Motor drives turbine and worm rotation, is driven by turbine and worm, and drive shaft 71 drives interlocking lever 73 to drive
Pressing plate 721 on pressing lever 72 presses centrifuge tube 11, ensure that sample is non-condensing on 13 inner wall of centrifuge tube；Open the second gas
Valve 621, by the second appendix 62 in babinet 1 into guide sleeve 61 supplying nitrogen, guide sleeve 61 in grinding head 12 to being passed through
Nitrogen, the nitrogen in guide sleeve 61 are entered by third venthole in grinding head 12, and nitrogen is overflowed by grinding head 12, emits outlet
Bubble, disturbed specimen freeze, so that sample is condensed into the graininess of 1mm-20mm, be conducive to sample and be fully ground, when reducing grinding
Between, improve grinding efficiency；Meanwhile nitrogen being made to overflow, the RNA degrading enzymes completely cut off in air are contacted with sample, and RNA is avoided to be dropped
Solution；It adjusts cantilever 13 and cam 22 adjusts the position of grinding head 12, centrifuge tube 11 is in when grinding head 12 being made to move to bottom
Bottom, and grinding head 12 is kept not contacted with 11 bottom of centrifuge tube, conflict is moved down when grinding head 12 being avoided to work arrives centrifuge tube 11
Bottom；Motor 21 is opened, so that 21 driving cam 22 of motor is driven first axle 23, the operating of the second axis 26, makes to connect with the second axis 26
12 positive and negative rotation of grinding head to form granular sample be stirred grinding, grinding head 12 move up and down to forming graininess
Sample carry out crush and grind, until sample powdered needed for being ground into；Aid pawl 31 is opened, motor 21, the first gas are closed
Valve 541, the second air valve 621 and other switches are opened after heating rod 34 melts condensate liquid 33 and close heating rod 34, and taking-up fills
The centrifuge tube 11 of required powdered samples completes RNA extraction grindings.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (7)
1. a kind of RNA milling and extractings system, it is characterised in that：Including babinet (1), centrifuge tube (11), grinding head (12), power mould
Block (2) grips module (3), heat preservation module (4), nitrogen circulation pressurize module (5), nitrogen import modul (6) and by pressing mold
Block (7), be provided on the babinet (1) can up and down adjustment cantilever (13)；The power plant module (2) is fixed on cantilever (13)
On；The grinding head (12) connect with power plant module (2), and power plant module (2) drives grinding head (12) positive and negative rotation, upper and lower straight line
Movement；The big end of grinding head (12) boring, grinding head (12) is equipped with the first venthole；It is described to grip module
(3) it is located on babinet (1), grips module (3) for babinet (1) to be fixed in centrifuge tube (11) aid；The heat preservation mould
Block (4) is used to keep the temperature centrifuge tube (11)；The nitrogen circulation pressurize module (5) is used for being passed through liquid nitrogen in babinet (1) and protecting
The interior nitrogen pressure of babinet (1) is held to stablize；The nitrogen import modul (6) and babinet (1) unicom, nitrogen import modul (6) are used for
To importing nitrogen in grinding head (12)；It is described to be connect with power plant module (2) by die block (7), it is used for centrifugation by die block (7)
Pipe (11) pressing makes sample be not adhere on centrifuge tube (11) inner wall.
2. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：The power plant module (2) includes
Motor (21), cam (22), first axle (23), the first axle sleeve (24), thrust ball bearing (25), the second axis (26) and the second axle sleeve
(27), the first axle (23) is adapted with the first axle sleeve (24)；The top of the first axle (23) is equipped with pulley；The pulley
Resetting spring is equipped between the first axle sleeve (24) (24)；Motor (21) driving cam (22) rotation, cam (22) pass through
Pulley drive first axle (23) moves downward, and resetting spring resets first axle (23)；The thrust ball bearing (25) is located at first
Between axis (23) and the second axis (26)；First axle (23) lower end is connect with thrust ball bearing (25) upper end, the second axis (26)
Upper end is connect with thrust ball bearing (25) lower end；It is provided with the first spiral chute on the outer curved wall of second axis (26)；Described
Two axle sleeves (27) inner wall is equipped with the first bulb, and the first bulb is adapted with the first spiral chute；Second axis (26) with
Second axle sleeve (27) rotational slide connects.
3. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：It is described to grip module (3) packet
It includes aid pawl (31), wet cloth (32), condensate liquid (33) and heating rod (34), the aid pawl (31) and is located at babinet (1) upper end simultaneously
It is connect with babinet (1)；The wet cloth (32) is located between aid pawl (31) and centrifuge tube (11), and wet cloth (32) edge extends to
Heat preservation module (4)；The condensate liquid (33) is located between centrifuge tube (11) and heat preservation module (4)；The stick of the heating rod (34)
Head is located in condensate liquid (33).
4. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：The heat preservation module (4) includes protecting
Warm slot (41) is provided with the second venthole (42), only logical plug (43) and the first pilot hole on the heat preservation tank (41), described only to lead to
It includes chock plug and stopper rod to fill in (43), and chock plug is linked together with stopper rod；The chock plug is located in the second venthole (42) and chock plug
The second venthole (42) is stretched out outside in end；The stopper rod is adapted with the first pilot hole；The first spring is arranged on the stopper rod；
First spring is between chock plug and the first pilot hole.
5. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：The nitrogen circulation pressurize module
(5) include the first nitrogen holding tank (51), the second nitrogen holding tank (52) and liquid nitrogen bottle (53), the first nitrogen holding tank
(51) with the left side unicom of babinet (1)；The right side unicom of the second nitrogen holding tank (52) and babinet (1)；First nitrogen
It is connected with the first appendix (54) between gas holding tank (51) and the second nitrogen holding tank (52)；On first appendix (54)
It is provided with the first air valve (541) and gasing pump (542)；The liquid nitrogen bottle (53) connect with gasing pump (542)；First air valve
(541) between gasing pump (542) and the second nitrogen holding tank (52).
6. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：Nitrogen import modul (6) packet
Include guide sleeve (61) and the second appendix (62), one end and babinet (1) unicom of second appendix (62), the second appendix
(62) the other end and guide sleeve (61) unicom；It is provided with the second air valve (621) on second appendix (62)；The air guide
Set (61) is set on grinding head (12)；The upper end of the grinding head (12) is provided with third venthole and third venthole is located at
In guide sleeve (61).
7. a kind of RNA milling and extractings system according to claim 1, it is characterised in that：It is described to include drive by die block (7)
Moving axis (71), pressing lever (72) and interlocking lever (73) are provided with the second spiral chute on the drive shaft (71)；The interlocking lever
(73) one end and pressing lever (72) vertical connection, the other end of interlocking lever (73) are provided with the second bulb；Second spiral
Sliding slot is adapted with the second bulb；It is driven by turbine and worm between the drive shaft (71) and power plant module (2)；The pressing
The end of bar (72) is provided with pressing plate flexible (721).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810202666.3A CN108435379B (en) | 2018-03-13 | 2018-03-13 | RNA grinds extraction system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810202666.3A CN108435379B (en) | 2018-03-13 | 2018-03-13 | RNA grinds extraction system |
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| CN108435379B CN108435379B (en) | 2020-01-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810202666.3A Active CN108435379B (en) | 2018-03-13 | 2018-03-13 | RNA grinds extraction system |
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| CN109682659A (en) * | 2019-01-14 | 2019-04-26 | 邢华铭 | A kind of Detecting Pesticide milling apparatus |
| CN110699242A (en) * | 2019-11-06 | 2020-01-17 | 张银冰 | RNA grinds extraction system |
| CN111036323A (en) * | 2018-10-11 | 2020-04-21 | 陕西前进齿科新技术开发有限公司 | Tooth detects with tooth reducing mechanism of being convenient for collect material |
| CN111672604A (en) * | 2020-06-25 | 2020-09-18 | 佟进伟 | Liquid nitrogen cryogenic crushing device |
| CN111974510A (en) * | 2020-07-15 | 2020-11-24 | 陈利强 | Small-size domestic medicinal material grinder of laborsaving formula of low noise |
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