CN108433106A - A kind of Oxygen-deficient endurance fructose powder (liquid) composition - Google Patents

Abstract

The present invention relates to field of health care food; more particularly to by active constituent D ribose, L arginine, taurine, American Ginseng medicinal extract, mono- carnitines of L, L-Ascorbic Acid L-O-Phosphate, polyfructosan and corrigent etc. at the health-care food being grouped as; rational prescription efficient combination ratio is selected by testing sieve; so that prescription effect generates synergistic effect; to effectively play the fatigue proof health-care effect of anti anoxia; it helps inferior health person or regains the enough energy needed for vital movement into plateau person, restore the due vigor of body.

Description

A kind of Oxygen-deficient endurance fructose powder (liquid) composition

Technical field

The present invention relates to a kind of Oxygen-deficient endurance fructose powder (liquid) compositions.

Background technology

Health food refers to having specific healthcare function or the food for the purpose of replenishing vitamins, minerals, is applicable in In specific crowd, have and adjust body function, not for the purpose for the treatment of disease, human body is not generated it is any it is acute, it is subacute or The food of person's chronic hazard.Health food is human medical as a kind of functional food between drug and ordinary food The important component of public health work.With the arrival in big healthy epoch, health food industry will welcome unprecedented development machine It meets.

Using this overall background as opportunity, to accelerate the paces of health food development, meet the health service need of consumers in general It asks, we are comprehensive to conclude prescription rule on the basis of the forefathers to different times study, and are exploitation with resist oxygen lack healthcare function It is directed toward, former achievements is excavated, conclude, summarize and are distilled, in conjunction with nutrient theory, theory of traditional Chinese medical science, cellular elements reason By the analysis of, cyto-dynamics scheduling theory, recreated from completely new angle.

But the environment on plateau has the unexistent feature in plains region：

First, oxygen content is low in the air of highlands.Result of study is shown, in 4300 meters of eminences of height above sea level, oxygen pressure Only the 58% of sea level.Therefore, although in the air on plateau, the content ratio of oxygen does not change, since plateau is whole The absolute magnitude of body rarefaction of air, oxygen has been small very much, and therefore, people from Plain enters plateau can lead to hypobaric hypoxia symptom mostly.

Followed by highlands very cold.For root it was found that often increasing 150 meters of height above sea levels, temperature will decline 1 degree； 1000 meters are often increased, 6.5 degree of mercury dropped.Therefore, the temperature of highlands is colder than other areas of Same Latitude.

Moreover it is exactly that humidity in the air of highlands is low.Plateau air is not only thin, but also very dry, in air Humidity is very low.Some researches show that people is daily 1.5 liters by breathing the moisture of discharge on plateau, the water being discharged by skin It is divided into 2.3 liters, under normal circumstances (in the case of not perspiring), body water exclusion amount increases by 1 than plains region human body discharge rate Times or more.

That the distinctive geographical conditions in highlands and natural environment are simply analyzed above, as air pressure is low, oxygen is forced down, It is cold low with humidity, moreover be exactly big, solar radiation strong (ultraviolet light and ionising radiation) of wind etc., these climatic characteristics are to Plain people Into after plateau, it will a series of influence of physiological functions occurs, it is most important to be presented with：The anoxic of nervous centralis, breathing system The various aspects symptom such as the anoxic of system, the anoxic of the circulatory system, anoxic of hematological system, people's habituation are referred to as altitude sickness.

In fact, human body anoxia is not only one type of high altitude anoxia.

Even in the people of plains region, also pathological hypoxic, physiological anoxic, motility anoxic and Environmental anoxic Etc. different types of hypoxic conditions.

Pathological hypoxic refer to more suffer from respiratory disease, cardiovascular and cerebrovascular disease patient, due to from external world's intake oxygen and logical The ability for crossing blood conveying oxygen declines, and causes body anoxic.Such as coronary heart disease, myocardial infarction, acute upper respiratory infection, chronic branch Tracheitis includes chronic obstructive pulmonary disease etc..

Physiological anoxic.Refer to advancing age, each organ is physiological aging, directly contributes human body intake oxygen amount drop It is low, it is reduced using the efficiency of oxygen, body is in the different chronic hypoxia state of degree.Lumen of vessels is narrow after people to old age, blood vessel Elasticity reduces, and blood flow is reduced, and lung capacity declines, and fortune function is greatly reduced.The elderly takes in oxygen amount and reduces, and transports the energy of oxygen Power reduces, and is declined using the efficiency of oxygen, and entire bodily tissue is in different degrees of anaerobic condition, and anoxic is exactly that the elderly is easy The pathologic basis that brain, the heart, Liver and kidney function reduce occurs.

Motility anoxic, nervous muscle power and mental activity can cause body oxygen consumption to increase, due to internal oxygen demand urgency Increase severely and add, merely by pulmonary respiration, is not enough to make up internal oxygen consumption, so that cell is in anaerobic environment, while adjoint A large amount of lactic acid generate, this is why the reason of being easy to have a pain in the back after the mental labour of a large amount of physical sports or anxiety.

Environmental anoxic, under normal circumstances, the oxygen content in air are 20.9%.If being in an oxygen content to be less than In 18% altitude environment, the oxygen of human body intake is insufficient, and the partial pressure of oxygen in blood is too low, and hemoglobin is in unsaturated shape The cell of state, each section tissue will show corresponding hypoxic conditions due to occurring certain variation for hypoxgia.Generally Come, every oxygen content is less than 20.9% environment, is all anaerobic environment.The even environment of slight hypoxia, gives birth to wherein for a long time Living, work, can also bring health different degrees of damage.The rarefaction of air on plateau, high-altitude, although in plateau, high-altitude In air, the ratio of oxygen is still 20.9%, but as height above sea level increases, and atmospheric density reduces, oxygen it is absolute Amount is also corresponding to be reduced.

The whatsoever anoxic of type is all a kind of pessimal stimulation to body, influences the various metabolic functions of body, especially The oxidative function of body is unbalance, and it is insufficient to eventually result in the supply of the major organs anoxic such as heart, brain, lung of body.

Therefore, the major function of anti-hypoxia health product is exactly to fight the anoxic of body, avoids the further development of lesion.When Preceding related resist oxygen lack product in the market is many, is broadly divided into two major classes：Chinese medicine class and bio-extract class.

Bio-extract class type is more, such as grape seed extract, anthocyanidin, flavones, saponin etc..And vitamins is most To be universal, vitamin can increase the oxygen carrying capacity of human body hemoglobin, improve blood oxygen saturation, have apparent enhancing to resist oxygen lack Effect, meanwhile, vitamins also has the function of antioxidant, can effectively remove the free radical that body generates under anaerobic environment, Improve Hypoxia toleration.

Second is that Chinese herbal medicine class.Such as rhodiola root, Radix Salviae Miltiorrhizae, ginseng, American Ginseng, matrimony vine, cordyceps sinensis, Radix Notoginseng Chinese medicine, including Using Chinese medicine active compound as the health products of single preparations of ephedrine and compound preparation, all there is different degrees of resisting oxygen lack.

The present inventor summarizes after the achievement of conscientious extensive research China and foreign countries forefathers：

1, theory of traditional Chinese medical science thinks the long-term chronic anoxic of each cell of human body, organ, is only and endangers the true of human life and health Positive " disease of danger ".Because theory of traditional Chinese medical science is to the importance of " gas " considerably beyond the theory of yin-yang and five elements (metal, wood, water, fire and earth), original theory of traditional Chinese medical science is Using yin-yang and five elements as theoretical foundation as, human body is regarded to the entity of ' gas, shape, god '." knack cured the disease is to understand gas Blood ", " vigour was both empty, must cannot reach in blood vessel, blood vessel must stop the interior stasis of blood without gas." if for the gas stasis of blood in internal organs, internal organs must acute disease Here is life.Theory of traditional Chinese medical science illustrates ' anoxic ' symptom that modern medicine is thought, in Chinese medicine from the correlation of ' gas ' and ' blood ' of human body In theory then be " qi being the governor of blood ", " blood being the material foundation of QI ", qi promoting blood production, gas can battalion blood vessels, the promoting circulation of blood of gas energy, QI can control blood, The qi and blood dialectical relationship of blood can carry QI.The impetus of gas is the power of blood, and the effect of taking the photograph of consolidating of gas makes among blood vascular It does not spill over outside arteries and veins.Blood again can be angry simultaneously, and blood constantly provides nutrition support for the generation of gas, and gas is present in blood, depends on blood Delivery and reach whole body, life and growth in nature in cycles.For example theory of traditional Chinese medical science, in discussing chronic obstructive pulmonary disease pathology, main judgement is with gas Based on void, it is believed that Lung Qi deficiency is the internal factor occurred.Phlegm, the stasis of blood are pathological products and pathogenic factor important in the course of disease.Think The lung-distension because inside having pent-up, first hindering lung qi, outer multiple sense is evil, and lung qi must not give vent to, then the lung-distension is made.Lung qi virtual loss, it is powerless to push The defeated cloth of operation of body fluid, body fluid stop gathering, then it is wet to give birth to phlegm unboiled water life.Spleen-Qi Deficiency, transportation capabilities imbalance, body fluid must not defeated cloth, gather and At phlegm.Phlegm coming from spleen, lung being reservoir of phlegm.In addition decline of kidney-YANG, disablement gas row water, and polywater is at phlegm；Phlegm sheet, water , it is derived from kidney, the work(of phlegm is wet, main in spleen；Phlegm trip, gas store in lung.Phlegm wet stops up resistance, and Qi dysfunction causes the stagnation of the circulation of vital energy, gas It is inverse；Again because of the mutual root alternate of qi and blood, then blood stasis, the turbid retardance of phlegm hinder blood to run, also cause blood stasis the stagnation of the circulation of vital energy.

In doctor trained in Western medicine theory, it is believed that oxygen reserve is few in human body, it is necessary to dependent on the supply of external environment oxygen and by exhaling The intake and transport of oxygen are constantly completed in suction, blood circulation, to ensure the needs of cell.When tissue cannot get abundance Oxygen, or when cannot make full use of oxygen, cell, tissue, the metabolism of organ, function, even morphosis may all be abnormal change Change, this pathologic process is known as anoxic.Such as cerebral anoxia.Under anaerobic conditions, the pathological change occurred at first is brain Make the forfeiture of current potential, extremely sensitive to anoxic, tolerance is worst.The current potential of nerve cell is only just opened for more than ten seconds after anoxic Begin to change.The mode that cytopathic effect inhibition assay shows under anaerobic condition is all that entanglement occurs for ion transport mechanism.Cellular energy source When glycometabolism, second is that the energy after energy-rich phosphate organic compound glycolysis.Sugar directly decomposes oxidation and generates free energy, human body A most important link is exactly atriphos (ATP) during interior anaerobic metabolism.ATP is an energy storages library, can be with It is energized directly to cell without slaking, oxidation.But the reserves of ATP are few in brain tissue, if under complete anaerobic condition, ATP just runs out of in a few minutes in brain tissue.There is no an ATP energy, sodium ion cannot be from being transferred to outside film into the cell, Bu Nengwei Normal film potential is held, cranial nerve cell just loses function.Sodium is about 40-44mmol/kg in normal human, extracellular The 44% of total sodium amount is accounted in liquid, and 9% is accounted in intracellular fluid, 47% is accounted in marrow.Internal sodium has exchangeable sodium and non-exchange sodium, Exchangeability accounts for 75%, and non-exchange sodium accounts for 25%, and the latter's calmness is in bone.Sodium ion holds extracellular fluid in extracellular fluid The maintenance of amount and osmotic pressure plays an important role, also critically important to the activity of muscle.Maintain intraor extracellular sodium concentration difference, mainly according to The effect of Na+-K+-ATP enzymes (i.e. Na+-K+ pumps) on cell membrane.Since the Na ion concentration outside cell membrane is than in film It is about three times larger, and potassium ion is higher than in film outside film, in the active procedure of nerve cell, potassium ion and sodium ion have temporarily A large amount of outflows.Potassium ion is since the concentration inside and outside film has favorable balance, so depending merely on variation and the osmotic pressure of permeability of cell membrane Difference, so that it may to be flowed into outside film out of film.And the case where sodium ion, is exactly the opposite, Na ion concentration is higher than outside film in film, sodium The outflow of ion is inversely to carry out, and depends merely on dispersion and mesh is not achieved.It must rely on a kind of mechanism of similar water pump that could carry out, As the water in well must can just be drunk up with water pump, energy, i.e. cellular energy ATP are needed.

Sodium balance disorder is often accompanied by the Balance disorders of water in body.The eubolism and balance of water and sodium are maintained in human body An important factor for ambient stable.If intracellular sodium ion is more and more, chlorion just enters in film, because chlorion is can be with Cell membrane is freely penetrated, positively charged sodium ion and electronegative chlorion meet and become sodium chloride, intracellular osmotic pressure It is continuously improved.Body can only allow moisture content to enter intracellular, moisture makes cell again to keep permeable pressure head inside and outside cell membrane to balance Volume increase, then cell starts oedema, and here it is generate brain edema after anoxic.Though human brain weight is only The 2% of weight, but its oxygen demand is a quarter of Whole body oxygen consumption to 1/5th.If it is acute anoxia, can also The increase for causing cerebral blood flow (CBF) causes vascular wall and brain Barrier Permeability to increase, this can also make brain cell interstitial edema, add The increase of sodium, water in brain cell, causes Brain circlulation by obstacle and causes brain edema, and here it is the originals for leading to altitude coma symptom Cause.In general, the main reason for edema it is anoxic, infection or acid poisoning.Its mechanism is：When anoxic mitochondria by Damage makes ATP generate and reduces, and then obstacle occurs for the sodium in cell membrane, potassium pump function, causes Na+ and water in endochylema to increase, together Shi Fasheng pathological changes.It is in vesica shape in swelling such as electric mitochondria intracytoplasmic under the microscope, endoplasmic reticulum；The observable under light microscopic It swells to diffusivity cell, endochylema is light to be contaminated, is limpid, and karyon is normal but sometimes slightly larger, and medically the cell of severe edema is referred to as Balloon sample becomes.The sodium in anoxic initial stage brain cell membrane, potassium pump function occur obstacle, and maintain the smooth of brain vessel blood in order to prevent It is logical, the outflow for promoting intracellular sodium ion is just must try to, the combination of chlorion and sodium ion is prevented, prevents the entrance of water.This It is Deterioration mechanism of the brain organ after anoxic, certainly, also that's about the size of it for the anoxic mechanism of other organs.

Lung organ for another example.Lung position highest in viscera, Chinese medicine claim lung covering other viscera, easily invade sense by exopathogen.The lobe of the lung It is delicate and can not resist cold heat, therefore it is referred to as saying for " tender dirty " to have lung.Think that lung is the position of storing soul, the master of QI；It is so-called ' in the green ', mainly The operation of lungs should be referred to freely.Because lung seeks hundred arteries and veins, the blood of whole body is all flowed through by hundred arteries and veins in lung, transpulmonary breathing, is carried out The exchange of the pure and impure gas in inside and outside, one exhales a suction, the pure and impure transporting of department.Some researches show that, Pulmonary Vascular to the reaction of anoxic with Body blood vessel is opposite.Alveolar hypoxia causes lung parteriole to shrink, and the alveolar blood flow of anoxic is made to reduce.Anoxic causes Pulmonary Vascular to shrink Mechanism it is more complex, theoretical point view is different, than if any sympathetic nerve effect say, it is believed that sympathetic activation caused by anoxic can be made Cause vascular contractile response for pulmonary vascular α receptors；There is humoral factor effect to say, it is believed that anoxic can promote loose in lung tissue Cell, pulmonary alveolar macrophage, vascular endothelial cell etc. discharge the vaso-active substances such as histamine, Prostaglandins and Leukotrienes, wherein What is had can shrink Pulmonary Vascular, some expansion blood vessels etc..There is theory to think that anoxic directly acts on vascular smooth muscle, because anoxic makes Smooth muscle cell membrane increases the permeability of Na+, Ca2+, promotes to flow in Na+, Ca2+, leads to myocyte's excitability and shrinkage Increase, it is possible to there is more blood plasma to filter out capillary and enter in lung tissue gap and alveolar, liquid in alveolar is made to gather, To form pulmonary edema.Hypoxic pulmonary vasoconstriction is the result of multifactor functioning in a word.

Under the related theoretical direction of traditional doctor trained in Western medicine, after to anti anoxia on the AD-targeted drugs of pulmonary hypertension, doctor trained in Western medicine It focuses mainly on reducing pulmonary hypertension.But while reducing pulmonary hypertension, (diastole) body circulation pressure is also reduced, To affect right ventricle perfusion and body circulation oxygen supply, certain important organ blood vessel dilatations are aggravated, have made the blood stasis of blood stagnant instead. When the severe depletion of oxygen of plateau, oedema can occur for interstitial lung, alveolar wall, cause oxygen that obstacle occurs to the disperse of cell tissue, serious to lack Oxygen can cause plateau pneumochysis.Anoxic is very big on the easypro contracting activity of local pulmonary circulation blood vessel influence, and acute or chronic anoxic can So that pulmonary circulation vessel retraction, resistance of blood flow is increased, pulmonary arteriole is caused to shrink extensively, pulmonary artery (partial circulating) pressure significantly increases, Pulmonary capillaries pressure also increases.And anoxia condition lower body systemic vascular diastolic pressure is just with pulmonary circulation pressure on the contrary, lung follows Ring pressure is high, and body circulation (systemic circulation) presses diastole.I.e.：Pulmonary Vascular (partial circulating) and body blood vessel are Pulmonary Vasculars to the direct reaction of anoxic It shrinks and body vasodilation.The diastole of anoxic aorta posterior, cerebral vasodilators, the former causes pulmonary hypertension, pulmonary heart disease, the latter to make Returned blood volume increases and promotes pulmonary encephalopathy occurrence and development.Western medicine uses vasodilator, will aggravate body vasodilation and brain blood Pipe further expansion.

Invention content

The purpose of the present invention is to provide a kind of definite ingredients, nontoxic to overcome the shortcomings of existing resist oxygen lack product technology The nutrient combination that can improve body fatigue resistance, resist oxygen lack.

The further domestic and international document in relation to anti-fatigue anoxia-tolerance of analysis, we, which are concerned about hypoxic conditions, can pass through difference Approach generate a large amount of free radical in vivo, since the property of free radical is very active, make the lipid mistake in internal cell membrane Oxidation, cell are in mixed acid toxic state, and body mitochondrial is damaged, and so that atriphos (ATP) is generated and reduce, cause Sodium balance is disorderly, and with the Balance disorders of body fluid (water), and carbon dioxide buildup, disperse are in intracellular and combined with cell body fluid Hydrogen ion and bicarbonate ion are generated, causes body tissue acidity to increase, body tissue is made to lose the balance of soda acid quickly, Cell pH value declines, oedema, and sodium, potassium pump function in cell membrane occur obstacle, causes Na+ and water in endochylema to increase, Jin Erfa A series of raw Hypoxic pathological changes.Anoxic makes smooth muscle cell membrane increase the permeability of Na+, Ca2+, promotes Na+, Ca2+ Interior stream causes myocyte's excitability to increase with shrinkage, and the degree bigger of the incomplete aerobic metabolism of body causes body tissue each Carbon dioxide cannot wash away away in time in organ, form the delay of carbon dioxide, and internal carbon dioxide buildup and anoxic are simultaneously deposited, This two groups of pessimum states and deposit, so that each organ of body especially respiratory apparatus, brain organ obstacle is occurred, pulmonary ventilation rate reduces, Cerebral blood flow (CBF) reduction, capilary Partial occlusion, pulmonary artery forms high pressure, or even brain edema, high altitude pulmonary edema, Combination occurs The appearance of the serious pessimum symptom such as acid poisoning.

The a large amount of free radicals generated in order to prevent by anoxic prevent the lipid peroxidation in internal cell membrane and mixed acid Poisoning symptom, it is necessary to intervene the health operating of the sodium, potassium pump function in anoxic incipient cell film in advance, and maintain each organ blood vessel Blood flow it is unimpeded, be the normal outflow for trying to promote intracellular sodium ion at hypoxic conditions initial stage, this be prevent hypoxic conditions after Supervention is opened up and the effective way of extension.We are while with reference to doctor trained in Western medicine theory, further according to theory of traditional Chinese medical science, with yin, yang, table, In, cold, heat, empty, the real eight principal syndromes usher into the dialectical Analyses of row defensive-qi-nutrient-blood.

Seldom it is simple and isolated though hypoxic conditions have respectively different exterior syndromes, but complicated close that be mutually related Therefore system should link up during dialectical and be analyzed, take into account the different mechanisms of traditional Chinese and western medicine theory, correctly judge, Comprehensive prescription means are taken, to carry out appropriate treatment

This prescription provide it is a kind of formula rationally, definite ingredients, nontoxic anti anoxia, fatigue proof health products, by activity Ingredient D-ribose, L-arginine, taurine, American Ginseng medicinal extract, mono- carnitines of L, L-Ascorbic Acid L-O-Phosphate, polyfructosan and rectify Taste agent etc. is into the health-care food being grouped as.The formula is new by looking into, and not yet at home and abroad pertinent literature retrieves the present invention Prescription document.

This prescription selects active constituent D-ribose, L-arginine, taurine, American Ginseng medicinal extract, mono- carnitines of L, vitamin C phosphorus Acid esters magnesium, polyfructosan select rational prescription efficient combination ratio, so that prescription effect as functional component by testing sieve Synergistic effect is generated, to effectively play the fatigue proof health-care effect of anti anoxia, inferior health person is helped or enters plateau person weight The new enough energy obtained needed for vital movement, restores the due vigor of body.

The corrigent is selected from the acceptable auxiliary material of food, and in particular national regulation can be used for the additive of food, preferably Following substance：Mannitol, sodium citrate, potassium citrate, Grape Skin be red, sodium benzoate, aspartame, honey element, xylitol, sweet tea Chrysanthemum glycosides, strawberry essence, erythrose alcohol, flavoring pineapple essence, orange flavor, peach flavor, green apple essence, watermelon essence, dioxy Change carbon etc., wherein optimization citric acid potassium, sodium citrate, aspartame, stevioside, strawberry essence, sodium benzoate.The above is rectified Taste agent substance, can be single substance, can also be two kinds or two or more mixtures.

According to this group of formula, each component is analyzed in conjunction with document, it is as a result as follows：

D-ribose：A kind of pentose is present in all cells, is the important of metabolism and energetic supersession in organism Substance.D-ribose is the constituent of the nucleoside derivates including the various RNA such as mRNA, RNA, 5sRNA and many coenzyme.Nine The ten's Mos, it has been found that D-ribose has the ability of fast quick-recovery musculature ATP synthesis.ATP is the general energy in human body Amount, when carrying out physical exertion and anoxic, ATP can be degraded to precursor substance, these precursor substances can be lost in from cell, So that ATP is unable to reach normal level in a short time, makes one to feel powerless, fatigue.It is any to cause muscle cell oxygen pressure local What anoxic reduced will loss cellular energy deposit.The reduction of energy can destroy the flow direction management of calcium, cause muscular tone, deadlock Firmly, pain and fatigue.The increase of intracellular Ca++ causes the loss of K+, this will stimulate on cell membrane feeling of pain by position device. Pain further increases muscular tone, aggravates energy loss, leads to vicious circle.Chronic ischemia, anoxic or frequent High pressure training can be such that purine is lost in from cell, and reduce cellular energy load.The frequency and severity of histanoxia Determine the speed and degree of energy loss.For unbred people, a high pressure training is just enough to exhaust energy storage It is standby, cause muscle rigidity and pain.For trained, more days frequent high intensity exertions also will produce it is this after Fruit.For sportsman, depleted of energy can cause to overtrain, and cause the physiological damage of myalgia, weakness and muscle.D- cores The intake of sugar can prevent the loss of ATP precursor substances, the rapid level for restoring body ATP, and body is enable to obtain life rapidly The required enough energy of life activity.High intensity after training, the level of ATP can reduce by 60% one 70% in musculature, Being restored to normal level needs 3 day time, if having eaten D-ribose, can in 24 hours the normal ATP levels of fast quick-recovery.D- Ribose can also make the fast quick-recovery ATP of the cardiac muscle for being subjected to ischemic injuries horizontal.When tissue surface is to Metabolic stress, ribose is supplemented Cell energy can be accelerated to restore, stimulation purine metabolism, hypoxic condition can cause muscle function and the various physiology shapes of performance Condition, including：

1, muscle rigidity, pain, weakness and restore slow from exercise；

2, cell membrane and muscle fiber damage；

3, oedema, swelling and electrochemistry are chaotic；

4, protease and phosphatidase attack cells；

5, the institutional frameworks such as free radical and damage cell membrane, mitochondria are formed；

6, Delayed Onset Muscle Soreness (DOMS)；

7, tired；

8, the synthesis such as hereditary macromolecular is postponed.

By keeping cell oxygen energy load, the loss of cell purine is controlled, increase oxygen energy is synthesized and remedied, ribose energy Enough mitigate these symptoms, accelerate to restore, and compensate due to overtrain or altitude environment caused by cell and tissue damage.

L-arginine：Known according to document, it is exogenous to give nitric oxide precursors L-arginine, hypoxic pulmonary can be alleviated Vascular adhesion molecule-1.Clinically start to treat lasting fetal circulation and meconium aspiration syndrome etc. using the method that NO is sucked Disease, and obtained preliminary efficacy.But NO suckings are costly, and instrumentation is complicated, therefore limits its clinical application.

L-arginine is the precursor of NO synthesis, it is catalyzed through NO synthase after entering in vivo and generates NO.In recent years about using L- The research that arginine intervenes hypoxic pulmonary vascular structural remodeling is more and more paid attention to, and many results of study show L- essences Propylhomoserin makes pulmonary arteries of hypoxic pulmonary hypertensive rats pressure be substantially reduced, and lung thin vessels flesh degree is substantially reduced, and lung is middle or small Flesh type artery RMT and RMA are substantially reduced, and pulmonary artery collagen expression is reduced, and the change of pulmonary artery ultra microstructure is relieved.

Experiment in vitro confirms that NO has direct repression to the proliferation of vascular smooth muscle cells, prompts L-arginine intervention One of the mechanism of hypoxic pulmonary vascular structural remodeling may be by the direct effect of NO；On the other hand, experiment in vitro is shown, NO The synthesis and secretion of huve cell ET-1 are can inhibit, and it is horizontal to reduce blood plasma ET-1.NO is to Chronic Hypoxic Rats lung Artery ET-1 gene expressions have inhibiting effect.And ET-1 is presently found strongest contracting vasoactive polypeptides in vivo, and have There is strong rush vascular smooth muscle cell proliferation.Thus prompt indirectly, L-arginine intervene hypoxic pulmonary vascular structural remodeling Another mechanism be by promote NO synthesis, to promote vascular smooth muscle cell proliferation factor ET-1 inhibition make With.

Taurine：Taurine is the cell-protecting of wide spectrum, has protective effect to cellular damage caused by anoxic.Taurine Anoxic body ET-1 and ACE secretion is can inhibit, increases NO and SOD levels, reduces pulmonary artery pressure, mitigate cardiopulmonary tissue damage. Taurine can weaken inhibited proliferation of the anoxic to pulmonary artery endothelial cell, and the rush arteria pulmonalis smooth muscle cells of anoxic is inhibited to increase The effect of growing is allowed to close to normal oxygen level.

Taurine is a kind of sulfur-containing amino acid in animal body, but is not protein constituent, also known as taurine, ox courage Element.The study found that taurine is the active material for adjusting body normal physiological activity, has the function of to maintain normal vision, maintain Body osmotic balance adjusts cell calcium balance, related with the mobility of cell membrane.Taurine is hypoglycemic, adjust nerve conduction, Endocrine Activity is participated in, lipid digestion is adjusted and absorbs, increase contractile ability, improve body immunity, enhancing cell The extensive biological actions such as film oxidation resistance, protection cardiac muscle cell.Taurine has the dynamic regulation and control of detoxication, calcium current, bile A variety of effects such as sour combination, osmotic pressure regulation and control, stabilizing cell membrane.There are anti-arrhythmia, myocardial preservation in cardiovascular system With the equal functions that reduce blood pressure.Relatively broad to action of the heart, exogenous supplement taurine can fight a variety of of heart Pathological state.Mechanisms of Taurine on Cardiaovascular System has a series of unique functions, mainly protects hypoxic-ischemic cardiac muscle, reinforces left ventricle Function increases myocardial contractive power, safeguards the stability of mitochondrial membrane of myocardium, anti-arrhythmia, prevents congestive heart failure, drop Low blood pressure, reduction cholesterolemia, raising high-density lipoprotein, atherosclerosis etc..And cell protective effect is then ox Sulfonic acid resist myocardial ischemia, the exposure basis of anti-arrhythmia, reperfusion injury, myocardial preservation etc..Taurine other Function also has lipid peroxidation product when can reduce movement, promotes the increase of body antioxidase activity after movement.It participates in Pulmonary Vascular caused by anoxic is reconstructed, and has certain preventive and therapeutic effect to hypoxic pulmonary hypertension.Taurine is that the cell of wide spectrum is protected Protect medicine, thus it is speculated that also have protective effect to cellular damage caused by anoxic.

Taurine can improve NO yield by protecting cells from anoxia-induced apoptosis.Research is it is also shown that taurine can make anoxic Caused L-arginine turn-over capacity obstacle is restored, and Transport Rate, nitric oxide synthetase (NOS) activity of L-arginine are made Increase, NO generations increase.NO also has other than adjusting antiotasis and inhibits blood platelet and haemocyte adherency, anti-lipid peroxy Change and protect the function of cell.

American Ginseng：American Ginseng is the dry root of araliaceae ginseng plant's American Ginseng, cool in nature, sweet in flavor, slight bitter, the thoughts of returning home, lung, Kidney channel has the effect of boosting qi and nourishing yin, clearing heat and promoting fluid.It is antifatigue, anti-that modern pharmacology research finds that American ginseng extract has Oxidation, resisting stress inhibit platelet aggregation, adjust the effects that blood glucose.Saponin component in American Ginseng can effectively enhance maincenter Nerve excitability, reach it is with all worries set aside with fixed attention, dispelling fatigue, enhancing memory the effect of, the rehabilitation to chronic fatigue syndrome With positive therapeutic potential.There are some researches prove American ginseng total saponins have function of resisting myocardial ischemia, and there are a variety of pharmacology to live Property, central nervous system function and heart body are such as adjusted, anti-arrhythmia cordis, resists myocardial ischemia, anti anoxia, antifatigue, anti-answer Swash, enhance the effects that immune function.It eliminates oxygen radical, and anti-lipid peroxidation reaction may be the two oxygen lack resistant function Main pharmacological mechanism.American Ginseng Central nervous has certain excitement and antifatigue effect, improves cell oxygen carrying capacity, enhancing breast The vigor of acidohydrogenase is conducive to the further oxygenolysis of lactic acid in blood, enhances immune system ability, and enhancing human body is externally got along well The resistance and adaptability of hypofunction caused by internal factor, that is, enhance stress ability, to improve resisting oxygen lack, The phagocytic function of macrophage is improved, the formation of body specific antibody is promoted, improves the division of T lymphocyte, bone-marrow-derived lymphocyte, The cytotoxicity for significantly increasing TIL cells stimulates the secretion of interleukin 2, enhancing natural killer cells (NKC) Activity improves the level of cyclic adenosine monophosphate.

L-carnitine：It is a kind of amino acid being widely present in human body, is the component part of food, it is considered to be human body generation The nutrient of required " biostearin " thanked.

1, " conversion " effect of l-carnitine

The main Physiological Function of l-carnitine is exactly that the FFA (free fatty) of transhipment activation enters mitochondrial matrix, and fat Main energy sources substance when fat is low-intensity prolonged exercise again, fat releases FFA by " mobilization " in adipocyte.FFA's Oxygenolysis mainly has 3 big steps：(1) it activates；(2) beta oxidation；(3) tricarboxylic acid cycle, rear 2 step carry out in mitochondria. FFA, which is entered by Skeletal Muscle Cell when mitochondrial oxidation decomposes, needs the help of carrier-carnitine on mitochondrial membrane that could enter, because It is not penetrating to FFA for mitochondrial inner membrane.The utilization of FFA can be enhanced by increasing the carnitine levels in muscle, in addition, the increasing of carnitine Adding can promote pyruvate oxidation to become acetyl-COA rather than receive NADH+H+ and be reduced into lactic acid, be generated to reduce lactic acid, And then improve sports achievement.

2, acyl group COA/COA ratios in mitochondria are adjusted, the oxidation of pyruvic acid is promoted

The short chain or Medium chain fatty acyl group COA that oxidation or other mitochondrial metabolism processes generate, pass through acyl group transfer reaction Fatty acyl carnitine is generated, fatty acyl carnitine transports mitochondria under the action of enzyme, is risen into the circulatory system and adjusts chondriosome acyl COA/ The effect of COA ratios.The stabilization of acyl group COA/COA ratios, plays an important role to energetic supersession in mitochondria.If mitochondrial matrix In acetyl group transport not in time, acetyl-COA/COA ratios rises, and has inhibiting effect to pyruvic dehydrogenase.Carnitine can improve The activity of pyruvic dehydrogenase promotes the oxidation of glucose to utilize, and the acyl group COA that can also improve branch is combined into carnitine Ester is conducive to its oxidation and utilizes to reduce inhibition of the excessive branched chain acyl COA to branch pyruvic dehydrogenase.

3, l-carnitine and Mitochondrial H+- ATP enzyme (F0F1ATP enzymes)

ATP levels are to determine the principal element of locomitivity in cell mitochondrial.As the enzymatic lysis of macromolecular, core be aggregated with And the formation of vesica, it is required for the participation of a large amount of ATP enzymes, the source of ATP mainly makes line grain by aerobic exercise in mitochondria Body oxidative phosphorylation, supplement carnitine add rational aerobic training that body mitochondria quantity is caused to increase, and the activity of ATP enzyme improves, The generation of ATP increases, and SOD activity significantly increases, and MDA contents decline, and lipid peroxide habituation, aerobic capacity obtains Improve, therefore cellular energy supply increases, and improves anti-fatigue ability.L-carnitine can increase skeletal muscle mitochondrial metabolic enzyme activity, Accelerate the clearance rate of free radical, the generation of delayed motion fatigue.Research has shown that supplement l-carnitine can make mouse power exhaust swimming The expression quantity of tricarboxylic acid cycle rate-limiting enzyme increases in skeletal muscle after movement, and oxidation resistance enhancing improves locomitivity.Widely Human body and zoopery show that l-cn can also reduce blood fat, promote myocardial metabolism and improve heart function, nerve conduction, surrounding The ischemia symptom of arterial disease, and the effects that improvement physical efficiency.

L-Ascorbic Acid L-O-Phosphate：L-Ascorbic Acid L-O-Phosphate advantageously forms and maintains collagen, keeps body all thin Born of the same parents combine, and vitamin C is the main matter of collagen, the important composition of cytoprotection layer connective tissue, but simultaneously calcium, Magnesium ion is also indispensable, as having condensation effect to pectin, calcium ions and magnesium ions vitamin C connective tissue can be made strong and It is flexible.Connective tissue is destroyed when hypovitaminosis C, is lacked calcium ions and magnesium ions and is allowed to become fragile.Cell just lacks at this time Defence capability.Ascorbic detoxication is and to invade the combination of human organism's Toxic, is gone out with homaluria.When people catches, Vitamin C content can be reduced and be disappeared in blood, and body disease-resistant can be helped by taking enough vitamin Cs.The vitamin C of large dosage The brittleness of Blood Cholesterol and capillary can be reduced.

Vitamin C can effectively block chain reaction caused by free radical, to play the role of remove free radical, have pair Play a part of antioxidant in body.

Magnesium ion in L-Ascorbic Acid L-O-Phosphate, as enzyme confactor participate in vivo all energetic supersessions, activation and The about a variety of enzyme systems of catalysis.Meanwhile magnesium ion has activated the adenyl cyclase on cell membrane, and pass through activated protein kinase And enzyme, stablize film potential, to release airway spasm, improves anaerobic condition, improve pulmonary circulation.Have been demonstrated that magnesium is almost participated in Each step of protein synthesis participates in the synthesis of immunoglobulin and participates in activating complement, adjusts the phagocytosis work(of phagocyte It can and adjust the maturation and function of lymphocyte.Therefore, magnesium ion also participates in adjusting body's immunity, increases disease resistance.

Activator of the magnesium as enzyme, enzyme are a kind of very important chemical substances in organism, are that one kind is generated by cell , specific proteins with catalytic activity and high specificity, it participates in and controls all metabolic processes in life, and Some enzymes can just be activated in the presence of must have metal ion, its catalysis of competence exertion, Mg2+ ions are exactly this fermentoid A kind of activator.

Magnesium and calcium have formed special partnership during producing atriphos (ATP).It is known that ATP It is a kind of bio-energy of preciousness in human body, is the power producer that people are engaged in activities, this online grain of important molecule It generates in vivo, is a kind of allantoid, very small " energy plant ", is deposited in inside living cells, if without it, it is living thin Born of the same parents just cannot get " can flow " of the required abundance of proper motion.Magnesium is cardiovascular umbrella, and expansible blood vessel prevents artery Sudden contraction makes blood pressure keep stablizing.Energy atherosclerosis, participates in the contraction process of cardiac muscle, when content of magnesium in cardiac muscle When reduction, myocardial necrosis can be caused.For a long time, magnesia mixture clinically be used to rescue various types of arrhythmia cordis, severe The patient of hypertension, hypertensive encephalopathy and acute myocardial infarction complicated heart failure.Magnesium has sedation to brain centres nerve, Nervous psychology can be eliminated, is eased off the pressure, impatience is released, helps and fall asleep.Magnesium has also undertaken the function of loosening all muscles, is called natural Tranquillizer can include the facial muscles often in tense situation with loose muscle.

Since above-mentioned several drugs are existing natural molecules in body, the equal nonhazardous of human body is acted on, can become anti-and lack The health products of the fatigue proof long-time service of oxygen.

Experiment 1, influence of the tested material to mouse entirety oxygen demand

1, material：

Reagent：

Tested material：Oxygen-deficient endurance fructose powder, trial target, 6 grams every bottle.

Positive control drug：Compound danshen dripping pills, lot number：160320, specification：The weight 27mg per ball, Tian Shili pharmacy group stock Part Co., Ltd.

Soda lime, Shanghai the May 4th chemical reagent Co., Ltd, specification：500g, product batch number：160807.

Experimental animal：ICR mouse, male, 22-24g, SCXK (capital) 2014-0013 are ground purchased from Chinese food drug assay Study carefully institute.

2, method：

Animal adaptability feed 1 week after be randomly divided into blank control group, compound danshen dripping pills control group (200mg/kg), Stoste control group (tested material B), tested material (A is dissolved in B) (0.5g/kg, 1g/kg, 2g/kg), every group 15, successive administration 15 days.

After last dose 1 hour, animal is put into the closed wide-mouth bottles of 250ml for being pre-loaded with 30g soda limes, uses silica gel The rubber stopper for being connected with buret is sealed with wide-mouth bottle, the buret other end is inserted into water.It records in 0-5min burets respectively Liquid level lifting height in buret when liquid level lifting height, death time of animal, death calculates the total oxygen consumption of survival period after experiment Amount/20g, 0-5min oxygen demand/20g.

3, data processing：

Data are indicated with mean+SD, and T-test inspections are carried out between group.

4, result：

When normobaric hypoxia, due to aoxidizing incomplete acidic metabolite, CO₂Accumulation, can stimulate chemocepter or in Pivot nerve, causes exaggerated respiration to be accelerated to reflectivity, and heartbeat is reinforced, and cardiac output increases, and blood is redistributed to ensure internal organs Blood supply.In this experiment under the conditions of normobaric hypoxia, by calculate special time period mouse oxygen demand, the death time and Survival period oxygen demand illustrates the resisting oxygen lack of tested material.

The experimental results showed that compared with Normal group, stoste control animals death time of tested material extended, Oxygen demand/20g and the total oxygen demand/20g of survival period increased in 0-5min, but statistics unknown significance difference (P ＞ 0.05), positive drug Danshen Root dropping ball and tested material 2g/kg groups, death time of animal are obviously prolonged, and the total oxygen demand/20g of survival period is bright It is aobvious to increase, the significant difference of statistics (P ＜ 0.05) (being shown in Table 1)

The influence (n=15) of table 1, tested material to mouse entirety oxygen demand

Compared with model group, * * P ＜ 0.01, * P ＜ 0.05

5, conclusion：

Tested material is under 2g/kg dosage, and successive administration is after 15 days, the difference in the determination experiment of mouse entirety oxygen demand Index has different degrees of resisting oxygen lack.

Experiment 2, influence of the tested material to mouse swimming time

1, material：

Reagent：

Tested material：A, white powder；B, black liquor.

Positive control drug：Compound danshen dripping pills, lot number：160320, specification：The weight 27mg per ball, Tian Shili pharmacy group stock Part Co., Ltd.

Soda lime, Shanghai the May 4th chemical reagent Co., Ltd, specification：500g, product batch number：160807.

Experimental animal：ICR mouse, male, 22-24g, SCXK (capital) 2014-0013 are ground purchased from Chinese food drug assay Study carefully institute.

2, method：

Animal adaptability feed 1 week after be randomly divided into blank control group, compound danshen dripping pills control group (200mg/kg), Stoste control group (tested material B), tested material (A is dissolved in B) (0.5g/kg, 1g/kg, 2g/kg), every group 10, successive administration 15 days.

After last dose 1 hour, by the iron wire for 9% weight of bearing a heavy burden with animal, it is put into depth of water 20cm, 30 DEG C of water temperature Swimming in bucket, observation mouse, which is swum to power, to exhaust and (cannot float within 10 seconds under water), when which is the swimming with a load attached to the body of mouse Between.

3, data processing：

Data are indicated with mean+SD, and T-test inspections are carried out between group.

4, result：

The generation of exercise induced fatigue is to cause the reduction of muscular contraction force caused by a series of biochemical changes of body by movement, The most direct performance of the generation of sports fatigue is the decline of body movement endurance.The time of swimming with a load attached to the body, which can be used as, in this experiment sentences The objective indicator of disconnected mouse muscle power strength, anti-fatigue ability.

Compared with Normal group, Danshen Root dropping ball (200mg/kg) group, tested material (1g/kg, 2g/kg) group compare model Group walking weight load is obviously prolonged, and statistics has pole significant difference (P ＜ 0.01).(being shown in Table 2)

The influence (n=10) of table 2, tested material to the mice burden swimming time

Compared with model group, * * P ＜ 0.01, * P ＜ 0.05

5, conclusion：

Under 1g/kg and 2g/kg dosage, successive administration has the mice burden swimming time and obviously prolongs tested material after 15 days Long effect, has certain antifatigue effect.

Experiment 3：Anxious toxicity test and the report of assessment Experimental Pathology

1, test sample and reference substance

1.1 test sample information

Title, abbreviated name, code name：Substituted phenyl-lactic acid fructose powder (oral solution)

Lot number：160708

Content or purity：60%~95%.

Physicochemical property and biological nature：Substituted phenyl-lactic acid fructose powder is white powder.

Preservation condition：(10-30 DEG C) preservation of room temperature

The term of validity：18 months

1.2 reference substances/experiment solvent, emulsifier and other medium informations

Test sample title, abbreviation, code name：Sodium carboxymethylcellulose, CMC-Na

Content or purity：Chemical pure (CP)

Physicochemical property and biological nature：White powder

Preservation condition：(10-30 DEG C) preservation of room temperature

Manufacturer：Sinopharm Chemical Reagent Co., Ltd.

The preparation of 1.3 test samples and reference substance

When preparing test sample or reference substance, wearing vest, wears masks at gloves.

The preparation of test sample：It uses 0.5% sodium carboxymethylcellulose as solvent, Substituted phenyl-lactic acid fructose powder is configured to dense Degree is the suspension of 0.125g/ml.Useful blender is stirred in process for preparation.

The preparation of reference substance：Appropriate sodium carboxymethylcellulose is weighed, distilled water is gradually added into, stirs evenly and is configured to 0.5% carboxymethylcellulose sodium solution.

The analysis of 1.4 test samples and reference substance measures

It is analyzed without test sample.

The processing of 1.5 test samples to keep sample with remaining test sample

Test sample keeps sample, and all peace is commented after the test in addition to the sample that keeps sample, and remaining test sample is returned by consigner's requirement Also.The remaining test sample preparation of experiment was returned to test articles handling department on the experiment same day and was uniformly processed.

The test sample amount that 1.6 this experiment use altogether is 10.0g.

2 experimental systems

2.1 animals use management and protection

Conservator is assessed and approved by U.S.'s International Laboratory Animal in national Beijing drug safety evaluation research center Meeting (Association for Assessment and Accreditation of Laboratory Animal Care International, AAALAC) International Certification, all animals in this research center are according to International Laboratory animal welfare behavior Criterion《Guide for the Care and Use of Laboratory Animals》It is required that carry out experimental animal use with Management.

2.2 experimental systems and selection reason

What People's Republic of China's national health and Family Planning Committee formulated《National food safety standard food security Property toxicological evaluation program》(GB15193.1-2014) rat is recommended.SD rats are the recognized standards in toxicologic study One of animal, this experiment use SD rats.Consigner also agrees to carry out acute toxicity test using this kind of animal.

2.3 experimental animal

Kind：Rat

System：SD

Animal level：SPF

Gender and quantity：Actual use female 20 (orders 23), and actual use male 20 (orders 23).

Animal age：7-8 weeks

The weight of animals range：Female Body Weight is ranging from the end of quarantine：146.1g~164.6g, it is male at the end of quarantine Property weight range is：143.2g~169.2g.

Animal origin：Beijing Vital River Experimental Animals Technology Co., Ltd.

Animal productiong licensing number and issuing unit：SCXK (capital) 2016-0011；Science and Technology Commission of Beijing

Animal quality certification number (note, quality certification upper right corner serial number)：No.11400700230613

Animal reaches and date received：2017-06-29

The raising of 2.4 experimental animals and management and its environmental condition

It raises in the Barrier Facility of national Beijing drug safety evaluation research center, Experimental Establishment quality certification approval number is SYXK- (army) 2012-0019, room temperature control illuminate, 12 hours dark for 12 hours in 20-25 DEG C, humidity 40-70%.Raising Mode is raised for rat box, and only per cage 2-5, cage for rearing poultry is the transparent mouse box of makrolon.Mouse box and pad twice are replaced weekly Material.

2.5 feeds and drinking water

Feeding Beijing Australia of section pull together feed corporation,Ltd production standard expanded pellet diet.Freely ingest.It is supplied with drinking bottle Enough reverse osmosis water (RO water), is freely drunk by animal.It is daily to replace cushion and drinking water.

2.6 quarantine procedures

All animals will be by the requirement of SOPA005 quarantine observation 3 days, within this time, the performances such as activity, diet of animal No abnormality seen, animal is preceding through clinical veterinarian passed examination in use, and provides inspection report.

The recognition methods of 2.7 experimental animals

After animal reaches, animal is had received by the requirement of SOPA005.An independent quarantine flowing water is determined to every animal Number.It is marked to animal by related SOPT019.When grouping a single experimental animal number is specified for every animal.Animal And indicate animal number on cage card.It is identified using experimental animal number in firsthand information.

Quarantine before grouping：It is unified to use green cage card, and indicate experiment numbers and individual animals number；

After grouping：Group differentiation is carried out with different colours cage card, and indicates experiment numbers, test sample title, dosage, group Not, gender, number of animals.

3 acute toxicity test of table is grouped animal identification

3, experimental design

The foundation of 1 experimental design

The data that 1.1 clients provide

The clinic trial scheme that client provides：

Quasi- dosage：People's recommended intake：0.05g/ kg body weights

Approach：It is oral

1.2 trial test results：Nothing

The file and document of 1.3 toxicity research guidelines

What State Food and Drug Administration worked out《Health food is examined and assessment technique specification》(May 1 in 2003 Day implement) and national health and Family Planning Committee formulation《National food safety standard food safety evaluation on toxicology Program》(GB15193.1-2014).

2 experiment groupings

4 acute toxicity test packet design of table

Solvent control group：It gives solvent 0.5% carboxymethylcellulose sodium solution with test sample group same volume, is administered every time Volume 20ml/kg is administered 3 times in 24 hours, amounts to 60ml/kg.

Given the test agent dosage group：Drug concentration：0.125g/ml, each administered volume 20ml/kg, 3 are administered in 24 hours It is secondary, amount to 60ml/kg, dosage is 0.125g/ml × 60ml/kg=7.5g/kg, and people's recommended intake is：0.05g/kg, by body Surface area calculates (method see reference document 4), is equivalent to 122 times of people's recommended intake.It is equivalent to people by kg body weight calculating 150 times (7.5g/kg/0.05g/kg) of quasi- dosage.

3 test samples and reference substance give

3.1 administration routes and method

Administered volume：Blank control group and given the test agent dosage group are 60ml/kg, 3 times a day, amount to 60ml/ daily kg。

Administration frequency and time：It is administered 3 times in 24 hours, dosing interval 4~6 hours.

The time limit is administered：It is repeatedly given in one day (3 times a day)

Administration way：Before experiment on an empty stomach, animal empty stomach overnight (not water restriction).According to SOPT001《Large and small mouse is oral Gastric infusion》Requirement operation.

The reasons why 3.2 administration routes and method choice

The selection reason of administration route：The clinical administration approach that client provides is oral, with the quasi- approach of people's clinic It is identical.

The selection reason of administered volume：20ml/kg is the larger administered volume of rat toxicity experiment gastric infusion, and rat fills Stomach maximum dosage-feeding is 20ml/kg, 20ml/kg/ times in this experiment, is given for 3 times, 60ml/kg/ days total.

The selection reason of administration frequency and time：《Health food is examined and assessment technique specification》Middle pertinent regulations.Daily When being administered multiple, interval time is 4~6 hours.

The selection reason in time limit is administered：It presses《Health food is examined and assessment technique specification》In regulation, acute toxicity gives The medicine time limit is 1 day.

4 observation indexs

4.1 general physical signs

On the day of gavage, close observation and the poisoning manifestations and feature, poison of each animal are recorded in a few hours especially after gavage Property reaction occur and recovery time and death condition etc., later daily observation 1 time, be observed continuously 14 days.Observation index includes Clinical symptoms (such as animal appearance, behavior, diet, the reaction to stimulation, secretion, excreta), death condition are (when dead Between, it is dying before reaction etc.), changes of weight (1 day before gavage, the 2nd day after gavage, respectively weigh within the 5th day, the 8th day, the 15th day one It is secondary, record the weight of animals variation, on the day of non-gavage animal dead or it is dying when should weigh) etc..All death conditions are recorded, are gone out The initial time of existing symptom and symptom, severity, duration, changes of weight etc..

4.2 pathological study indexs and time

Gross anatomy carried out to all experimental animals, including within experimental period it is dead, be euthanized and try because dying The animal put to death at the end of testing.When gross anatomy occurs abnormal, it should all record and carry out histopathological examination.

5 detection methods

5.1 body weight measurement

By SOPT006《The weighing standard practice instructions of rats and mice weight》, with Mettler-PB3001 electronic balances (Mei Te Le-support benefit (Changzhou) Weight Equipment System Co., Ltd) measure weight.

5 statistical analyses

To weight, average value and standard deviation are calculated by group and gender.

4, brief summary：

General physical signs observation

Death condition：Without animal dead during experiment, all animals are living to deposit to when plan postmortem.

Clinical sign：After the 1st gavage, during the 2nd time and the 3rd gavage, given the test agent dosage group animal observes There is a loose stools symptom, second day animal loose stools symptom disappears after gavage, and animal restores normal.Solvent control group animal clinical symptom is not See exception.

Rat oral gavage Substituted phenyl-lactic acid fructose powder (oral solution) urgency toxicity test, solvent control group and given the test agent dosage group Pathologic finding shows no obvious abnormalities change.

Referring to subordinate list 1 and subordinate list 3.

Weight：Average weight has no and the relevant effect of test sample processing.Differential magnitude between control group is slight, not Reach significance,statistical level.

Referring to subordinate list 2 and subordinate list 4, attached drawing 1,2.

5, histopathologic examination

Animal system postmortem：Substantially postmortem when have no and the relevant exception of test sample.

Referring to attached report 1.

Under the conditions of determined by this experiment, tested material Substituted phenyl-lactic acid fructose powder (oral solution) is to the oral acute of rat Toxicity dose is more than 7.5g/kg BW, LD₅₀More than 7.5g/kg BW, 7.5g/kg BW are equivalent to the 150 of people's recommended intake It (is calculated again by kg body weight).

6, statistical graph

1 clinical sign of subordinate list observes result statistical form

2 the weight of animals measurement result statistical form of subordinate list

Note：Data are in table

Description of the drawings

Attached drawing 1：Jenny changes of weight.Attached drawing 2：Buck changes of weight.

7, animal individual test data subordinate list

3 individual animals clinicing symptom observation result of subordinate list

4 individual animals body weight determination result of subordinate list

The advantage of the invention is that：

1, it is proven by putting into practice above, raw materials used is food additives or pharmaceutical grade raw material, and equal For existing natural molecule in body, effect harmless to the human body can become the health products of the fatigue proof long-time service of anti anoxia.

2, prescription of the present invention selects rational prescription efficient combination ratio by testing sieve, so that prescription effect generates collaboration Effect, D-ribose, L-arginine, taurine, American Ginseng medicinal extract, l-carnitine, L-Ascorbic Acid L-O-Phosphate joint prescription have it is excellent Gesture, the more than simple addition of several drugs, they, which mutually act synergistically, increases mutual function, to effectively play anti anoxia Fatigue proof health-care effect.

Specific implementation mode

A kind of Oxygen-deficient endurance fructose powder (liquid) composition is further illustrated the present invention by following specific examples, it is excellent It can be oral administration solution to select preparation, can also be solid dosage forms, including solid tablet, solid particle dosage form, effervescent tablet and solid Powder form, the present embodiment are only to illustrate the present invention, are not the limitation to other dosage forms of the present invention.

It is indicated with prescription compages weight ratio, each main group of a kind of Oxygen-deficient endurance fructose powder (liquid) composition of the present invention At weight ratio following (gauge used with 6g/1000 vial formulation units, is declared again, said preparation unit should not be constituted to this The limitation of invention)：

American Ginseng medicinal extract 50-200g, L carnitine 200-1000g, D-ribose 300-1000g, L-arginine 50-500g, dimension Raw element C phosphate magnesium 200-1000g, taurine 100-800g, crystal diabetin 500-5000g, oligofructose 500-5000g, sweet tea Sweet element 10-50g, potassium sorbate 30-50g, essence 0.6-2g, appropriate citric acid.

It is preferred that a kind of weight ratio of each composition of Oxygen-deficient endurance fructose powder (liquid) composition of the invention is：

American Ginseng medicinal extract 100-200g, L carnitine 500-1000g, D-ribose 400-600g, L-arginine 200-500g, dimension Raw element C phosphate magnesium 400-600g, taurine 300-600g, crystal diabetin 1000-4000g, oligofructose 1000-4500g, Honey element 10-50g, potassium sorbate 30-50g, essence 0.6-2g, appropriate citric acid.

Embodiment 1：For the weight ratio for being prepared into each composition of pulvis is (by 6g/1000 bottles)：

American Ginseng medicinal extract 100g, L carnitine 500g, D-ribose 400g, L-arginine 300g, vitamin C phosphate magnesium 400g, taurine 300g, crystal diabetin 1500g, oligofructose 2420g, honey element 50g, potassium sorbate 30g, appropriate citric acid.

Preparation flow：Raw material → examination → freezer → mark Huaihe River dispensing → ultraviolet-sterilization → crushing → sieving → centre storage Deposit tank → automation finished product → gland → sterilizing → secondary package → storage.

Embodiment 2：For the weight ratio for being prepared into each composition of pulvis is (by 8g/1000 bottles)：

American Ginseng medicinal extract 150g, L carnitine 600g, D-ribose 600g, L-arginine 400g, vitamin C phosphate magnesium 500g, taurine 400g, crystal diabetin 2500g, oligofructose 2770g, honey element 50g, potassium sorbate 30g, appropriate citric acid.

Preparation flow：Raw material → examination → freezer → mark Huaihe River dispensing → ultraviolet-sterilization → crushing → sieving → centre storage Deposit tank → automation finished product → gland → sterilizing → secondary package → storage.

Embodiment 3：For the weight ratio for being prepared into each composition of pulvis is (by 10g/1000 bottles)：

American Ginseng medicinal extract 150g, L carnitine 600g, D-ribose 600g, L-arginine 400g, vitamin C phosphate magnesium 600g, taurine 400g, crystal diabetin 3600g, oligofructose 3570g, honey element 50g, potassium sorbate 30g, appropriate citric acid.

Preparation flow：Raw material → examination → freezer → mark Huaihe River dispensing → ultraviolet-sterilization → crushing → sieving → centre storage Deposit tank → automation finished product → gland → sterilizing → secondary package → storage.

Embodiment 4：It is prepared into for the weight ratio of each composition of two kinds of dosage forms of A, B agent i.e. pulvis and oral solution：

Using A, B agent as dosage form, wherein：A agent is liquid preparation；B agent is powder preparation form：(no matter A agent and B agent, Respectively with 1000 bottles be preparation unit use gauge, wherein A agent by 25ml/ bottles, B agent in terms of 6 grams/bottle)：

1, A agent：

American Ginseng medicinal extract 100g, l-carnitine 500g, honey element 35g, potassium sorbate 70g, appropriate citric acid, deionized water 25000ml。

If preparation does not have American Ginseng medicinal extract, American Ginseng crude drug 1000g can be used, adds water retting 1 hour, decoction 2 times, often Secondary 2 hours, collecting decoction, filtration, filtrate is concentrated, be made relative density be 1.08 concentrate, after cooling be added l-carnitine, Potassium sorbate is added deionized water to total amount, is stirred evenly between citron acid for adjusting pH value 4.5~5.5, stands 24 hours, filtering, It is distributed into 30ml bottles, seals, with 115 DEG C of pressure sterilizing 30min.

2, B agent：

D-ribose 400g, L-arginine 300g, L-Ascorbic Acid L-O-Phosphate 400g, taurine 300g, citric acid 110g, knot Brilliant fructose 2300g, oligofructose 2190g.

D-ribose, L-arginine, L-Ascorbic Acid L-O-Phosphate, taurine, citric acid, crystal diabetin, oligofructose are mixed It closes, 80 mesh of uniform mistake sieves 5 times, and the raw material that mixing is sieved under ultraviolet light be kept in dark place by sterilization packaging, sealing.

Physical and chemical index：PH value 4.5-5.5, lead (in terms of Ps)≤0.5mg/kg, arsenic (in terms of As)≤0.3mg/kg, food additive Add agent that should meet the regulation of GB2760.

Microbiological indicator：Total number of bacteria (cfu/ml)≤100, coliform (MPN100ml/)≤6, mould (cfu/ml) ≤ 10, saccharomycete (cfu/ml)≤10, pathogenic bacteria (referring to pathogenic entero becteria and pathogenic coccus) must not detect.

A kind of Oxygen-deficient endurance fructose powder (liquid) composition of the present invention, is indicated with prescription compages weight ratio, composition it is each In the weight of a composition, former, supplementary product consumption is to be made the amounts of 1000 preparation units as formula ratio, such as to be made 1000 Grain, 1000,1000 bags, 1000 milliliters, the amount of supplementary material used in 1000 bottles etc. calculate formula ratio, be not formulated as a percentage Amount.

This prescription Oxygen-deficient endurance fructose powder (liquid) composition, can be prepared into according to different additives liquid preparation or Person's solid pharmaceutical preparation can also be tablet and capsule preparations.In fact this prescription Oxygen-deficient endurance fructose powder (liquid) composition, no matter Using liquid preparation or solid pharmaceutical preparation, ideal healthcare function, as long as being taken by medium of water, whatsoever agent can get Type can make the composition give full play to curative effect, so that the crowd under anoxic or fatigue state is obtained physical efficiency faster extensive It is multiple.

This prescription Oxygen-deficient endurance fructose powder (liquid) composition, preferred formulation can be oral administration solution, can also be solid Dosage form, including solid tablet, solid particle dosage form, effervescent tablet and solid powder dosage form, the present embodiment are only to illustrate the present invention, It is not the limitation to other dosage forms of the present invention.

This prescription selects D-ribose, L-arginine, taurine, American Ginseng medicinal extract, l-carnitine, vitamin C phosphate magnesium to make For functional component, and reasonable efficient combination ratio is selected by testing sieve, prescription effect of this prescription is made to generate synergistic effect, from And the fatigue proof health-care effect of anti anoxia is effectively played, it helps body to regain the enough energy needed for vital movement, restores The due vigor of body.

Claims (4)

1. a kind of Oxygen-deficient endurance fructose powder (liquid) composition, it is characterised in that：With weight ratio meter, the Oxygen-deficient endurance fructose powder (liquid) composition main component includes American Ginseng medicinal extract 50-200g, L carnitine 200-1000g, D-ribose 300-1000g, L- essence ammonia Sour 50-500g, L-Ascorbic Acid L-O-Phosphate 200-1000g, taurine 100-800g, crystal diabetin 500-5000g, oligofructose 500-5000g and corrigent.

2. a kind of preparation method of Oxygen-deficient endurance fructose powder (liquid) composition according to claim 1 is：It is prepared into Method when pulvis is：American Ginseng medicinal extract 100g, L carnitine 500g, D-ribose 400g, L-arginine 300g, vitamin C phosphoric ester Magnesium 400g, taurine 300g, crystal diabetin 1500g, oligofructose 2420g, honey element 50g, potassium sorbate 30g, citric acid are suitable Amount.Preparation flow：Raw material → examination → freezer → mark Huaihe River dispensing → ultraviolet-sterilization → crushing → sieving → intermediate storage tank → automation finished product → gland → sterilizing → secondary package → storage.

3. a kind of preparation method of Oxygen-deficient endurance fructose powder (liquid) composition according to claim 1 is：It is prepared into Oral solution and the method for two kinds of dosage forms of solid powder are：Using A, B agent as dosage form, wherein：A agent is liquid preparation, and B agent is powder Agent formulation form：A, A agent：American Ginseng medicinal extract 100g, l-carnitine 500g, honey element 35g, potassium sorbate 30g, appropriate citric acid, The above raw material is mixed and is filtered by deionized water 25000ml, l-carnitine, potassium sorbate is then added, with citron acid for adjusting pH value Between 4.5~5.5, add deionized water to total amount, stir evenly, stand 24 hours, filtering is distributed into 30ml bottles, is sealed, with 115 DEG C pressure sterilizing 30min.B, B agent：D-ribose 400g, L-arginine 300g, L-Ascorbic Acid L-O-Phosphate 400g, taurine 300g, citric acid 110g, crystal diabetin 2300g, oligofructose 2190g.The above raw material is mixed, 80 mesh of uniform mistake sieves 5 times, will Mixing the raw material being sieved, sterilization filling is packed under ultraviolet light, and sealing is kept in dark place.

4. a kind of Oxygen-deficient endurance fructose powder (liquid) composition according to claim 1,2,3, it is characterised in that：The present invention Preferred formulation can be oral administration solution, can also be solid dosage forms, including solid tablet, solid particle, solid powder dosage form, It is only to illustrate the present invention in the embodiment of the present invention, is not the limitation to other dosage forms of the present invention.