CN108431227A - 使用微核醣核酸前驱物作为诱导cd34阳性成体干细胞增殖的药物 - Google Patents
使用微核醣核酸前驱物作为诱导cd34阳性成体干细胞增殖的药物 Download PDFInfo
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562262280P | 2015-12-02 | 2015-12-02 | |
| US62/262,280 | 2015-12-02 | ||
| US15/048,964 | 2016-02-19 | ||
| US15/048,964 US10519440B2 (en) | 2012-08-10 | 2016-02-19 | Composition and method of using miR-302 precursors as drugs for treating Alzheimer's diseases |
| US15/167,226 US9879263B2 (en) | 2011-08-12 | 2016-05-27 | Use of microRNA precursors as drugs for inducing CD34-positive adult stem cell expansion |
| US15/167,226 | 2016-05-27 | ||
| PCT/US2016/049583 WO2017095489A1 (en) | 2015-12-02 | 2016-08-31 | Use of microrna precursors as drugs for inducing cd34-positive adult stem cell expansion |
Publications (1)
| Publication Number | Publication Date |
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| CN108431227A true CN108431227A (zh) | 2018-08-21 |
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| CN201680070742.9A Pending CN108431227A (zh) | 2015-12-02 | 2016-08-31 | 使用微核醣核酸前驱物作为诱导cd34阳性成体干细胞增殖的药物 |
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| Country | Link |
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| EP (1) | EP3384026A1 (enExample) |
| JP (1) | JP2018535684A (enExample) |
| CN (1) | CN108431227A (enExample) |
| TW (1) | TWI720075B (enExample) |
| WO (1) | WO2017095489A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112430596A (zh) * | 2019-08-26 | 2021-03-02 | 中国科学院上海营养与健康研究所 | 一类小rna分子及其类似物在抗衰老中的应用 |
| CN112912492A (zh) * | 2018-07-02 | 2021-06-04 | 林希龙 | 活体外诱导成体干细胞增殖及分化 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11624067B2 (en) | 2008-05-07 | 2023-04-11 | Shi-Lung Lin | In-vitro induction of adult stem cell expansion and derivation |
| US20220396778A1 (en) * | 2021-06-12 | 2022-12-15 | Shi-Lung Lin | Novel RNA Composition and Production Method for Use in iPS Cell Generation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101970661A (zh) * | 2007-10-29 | 2011-02-09 | 林希龙 | 使用内含子的核糖核酸生成人类类胚胎干细胞 |
| CN101970664A (zh) * | 2008-01-16 | 2011-02-09 | 林希龙 | 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞 |
| WO2013025248A1 (en) * | 2011-08-12 | 2013-02-21 | Mello Biotechnology, Inc. | Inducable expression from the eukaryotic pol -2 promoter in prokaryotes |
| WO2014026189A2 (en) * | 2012-08-10 | 2014-02-13 | Shi-Lung Lin | Production and utilization of a novel anti-cancer drug in therapy |
| WO2014106011A1 (en) * | 2012-12-28 | 2014-07-03 | Shi-Lung Lin | Production and extraction of microrna precursor as drug for cancer therapy |
| US20150132805A1 (en) * | 2012-08-10 | 2015-05-14 | Mello Biotech Taiwan Co., Ltd. | Composition for producing microrna precursors as drugs for enhancing wound healing and production method of the microrna precursors |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2088188T3 (da) | 1997-04-16 | 2012-01-02 | Unigene Lab Inc | Direkte ekspression af peptider i dyrkningsmedier |
| WO2006073846A2 (en) | 2004-12-22 | 2006-07-13 | Ambrx, Inc. | Methods for expression and purification of recombinant human growth hormone |
| US9879263B2 (en) * | 2011-08-12 | 2018-01-30 | Mello Biotechnology, Inc. | Use of microRNA precursors as drugs for inducing CD34-positive adult stem cell expansion |
-
2016
- 2016-08-31 JP JP2018528746A patent/JP2018535684A/ja active Pending
- 2016-08-31 WO PCT/US2016/049583 patent/WO2017095489A1/en not_active Ceased
- 2016-08-31 CN CN201680070742.9A patent/CN108431227A/zh active Pending
- 2016-08-31 EP EP16763666.1A patent/EP3384026A1/en not_active Withdrawn
- 2016-12-02 TW TW105139971A patent/TWI720075B/zh active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101970661A (zh) * | 2007-10-29 | 2011-02-09 | 林希龙 | 使用内含子的核糖核酸生成人类类胚胎干细胞 |
| CN101970664A (zh) * | 2008-01-16 | 2011-02-09 | 林希龙 | 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞 |
| WO2013025248A1 (en) * | 2011-08-12 | 2013-02-21 | Mello Biotechnology, Inc. | Inducable expression from the eukaryotic pol -2 promoter in prokaryotes |
| WO2014026189A2 (en) * | 2012-08-10 | 2014-02-13 | Shi-Lung Lin | Production and utilization of a novel anti-cancer drug in therapy |
| US20150132805A1 (en) * | 2012-08-10 | 2015-05-14 | Mello Biotech Taiwan Co., Ltd. | Composition for producing microrna precursors as drugs for enhancing wound healing and production method of the microrna precursors |
| WO2014106011A1 (en) * | 2012-12-28 | 2014-07-03 | Shi-Lung Lin | Production and extraction of microrna precursor as drug for cancer therapy |
Non-Patent Citations (2)
| Title |
|---|
| BAOJIAN LIAO: "MicroRNA Cluster 302–367 Enhances Somatic Cell Reprogramming by Accelerating a Mesenchymal-to-Epithelial Transition", 《THE JOURNAL OF BIOLOGICAL CHEMISTRY》 * |
| MING YAN: "Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs", 《PLOS ONE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112912492A (zh) * | 2018-07-02 | 2021-06-04 | 林希龙 | 活体外诱导成体干细胞增殖及分化 |
| CN112430596A (zh) * | 2019-08-26 | 2021-03-02 | 中国科学院上海营养与健康研究所 | 一类小rna分子及其类似物在抗衰老中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| TWI720075B (zh) | 2021-03-01 |
| TW201735932A (zh) | 2017-10-16 |
| WO2017095489A1 (en) | 2017-06-08 |
| JP2018535684A (ja) | 2018-12-06 |
| EP3384026A1 (en) | 2018-10-10 |
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Application publication date: 20180821 |