CN108426973A - The method of quality control of Chinese medicine lizard and its application - Google Patents
The method of quality control of Chinese medicine lizard and its application Download PDFInfo
- Publication number
- CN108426973A CN108426973A CN201710080187.4A CN201710080187A CN108426973A CN 108426973 A CN108426973 A CN 108426973A CN 201710080187 A CN201710080187 A CN 201710080187A CN 108426973 A CN108426973 A CN 108426973A
- Authority
- CN
- China
- Prior art keywords
- lizard
- chinese medicine
- ethyl acetate
- quality control
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a kind of traditional Chinese medicine quality control methods, and in particular to arrives the method for quality control of lizard.Using lizard as control medicinal material product as a contrast, test solution and reference substance solution are taken respectively, point is on same silica gel g thin-layer plate, with ethyl acetate hexane（4:1）For solvent, spray in test sample chromatography, on position corresponding with control medicinal material chromatography, shows the spot of same color with 10% ethanol solution of sulfuric acid.The method specificity of the Chinese medicine lizard quality control of the present invention is strong, and sensitivity, stability are good.
Description
Technical field
The present invention relates to a kind of traditional Chinese medicine quality control methods, and in particular to arrives the method for quality control of lizard.
Background technology
Application of the animal drugs in China has long history, and lizard, as animal drugs application, Medicinal has for a long time
History, it is curative for effect.By investigation, the lizard mainstream kind in medicinal material market is Scincidae skink and Lacertide at present
Beautiful class fiber crops lizard.Skink as " lizard " first recorded in《Sheng Nong's herbal classic》,《Compendium of Materia Medica》In it is also on the books, it is logical with Li Shui
Leaching, broken knot dissipate the stasis of blood, removing toxic substances effect, be distributed in the Yangtze river basin and areas to the south.Eremias argus as " lizard " first recorded in《Jilin
Chinese herbal medicine》, it is used as medicine with entirety, is killed by a pinch after summer and autumn capture, dry or used after drying；With promoting blood circulation, eliminating goiter and stasis, removing toxic substances
The effects that calm, modern clinic are also chiefly used in treating chronic bronchitis, osteomyelitis, bone tuberculosis and scrofula, are distributed in me
The ground such as state northeast and Gansu, Hebei, Shandong, Shanxi, Shaanxi, Qinghai.
Lizard is few as animal drugs, its quality controling research, is lizard at present still without feasible quality control standard
Application make troubles, lead to the uncontrollability of quality.Chinese patent CN200710185654.6 discloses a kind of Cabrite medicinal materials
Discrimination method, but the solvent used is toluene or benzene, toluene are the easy malicious reagent of system, and benzene has a high toxicity, and this method
Solvent use n-hexane and ethyl acetate, more environmentally friendly safety.
Invention content
Goal of the invention：Problem to be solved by this invention is to provide that a kind of index components feature is apparent, easy to operate, cost
It is low, labor intensity is low, quick and precisely carry out Chinese medicine lizard quality control method.
The purpose of the present invention is achieved through the following technical solutions：
The method of quality control of Chinese medicine lizard, it includes the following steps：
Step（1）：Lizard powder 1g is taken, adds ethyl acetate 20ml ultrasonic extractions 30 minutes, is filtered, filtrate, which is placed in water-bath, steams
Dry, residue adds ethyl acetate 1ml to make dissolving, as test solution.Lizard control medicinal material 1g separately is taken, is made in the same way of control medicinal material
Solution.
Step（2）：According to thin-layered chromatography（Chinese Pharmacopoeia one general rule 0502 of version in 2015）Lizard test sample is drawn in experiment
Solution and each 5 μ l of control medicinal material solution are put respectively on same silica gel g thin-layer plate, with n-hexane-ethyl acetate（4:1）For exhibition
Agent is opened, is unfolded, takes out, dries, sprays with 10% ethanol solution of sulfuric acid, it is clear to be heated to spot development at 105 DEG C.Test sample chromatography
In, on position corresponding with control medicinal material chromatography, show the principal spot of same color.
Preferably, the method for above-described Chinese medicine lizard quality control, the extraction solvent wherein in step 1
For ethyl acetate, chloroform, wherein being preferably ethyl acetate.
Preferably, wherein step（1）In extracting mode be ultrasonic extraction 20min, 30min, wherein preferably
For ultrasonic extraction 30min.
Preferably, wherein step（2）In solvent be n-hexane-ethyl acetate（4:1）.
Preferably, wherein step（2）In color developing agent be 10% ethanol solution of sulfuric acid, 10% phosphomolybdic acid ethyl alcohol,
In relatively optimization be 10% ethanol solution of sulfuric acid.
Preferably, wherein step（2）In colour temp be 105 DEG C heating develop the color.
Skink as " lizard " first recorded in《Sheng Nong's herbal classic》,《Compendium of Materia Medica》In it is also on the books, have promoting diuresis for stranguria,
Broken knot dissipate the stasis of blood, removing toxic substances effect.
Eremias argus as " lizard " first recorded in《Jilin Chinese herbal medicine》, it is used as medicine with entirety, kills by a pinch, dry after summer and autumn capture
Or it is used after drying；With promoting blood circulation, eliminating goiter and stasis is detoxified the effects that calm, and modern clinic is also chiefly used in treating Chronic Airway
Inflammation, osteomyelitis, bone tuberculosis and scrofula.
Therefore the present invention selects the lizard mainstream kind Scincidae skink in current medicinal material market and the beautiful class fiber crops of Lacertide
Test medicinal material of the lizard as lizard method of quality control.
Reunion of bone capsule has the function of promoting blood circulation and removing blood stasis, swelling and pain relieving.For soft tissue injury, fracture.The scattered stasis of blood is dispelled blood,
Continuous muscles and bones treat internal injury stasis caused pain, contain lizard in prescription, as test medicine.
The method of quality control of Chinese medicine lizard provided by the invention has the following advantages compared with prior art：
The present invention provides the method for quality control of Chinese medicine lizard, selects mainstream kind as experiment medicinal material, molten by optimizing extraction
Agent selects suitable solvent, colour developing mode, ensures that experimental result is accurate, and method specificity is strong, favorable reproducibility, is suitable for institute
Have the Cabrite medicinal materials in source discriminating and containing in all source Cabrite medicinal materials manufactured goods whether the discriminating containing Cabrite medicinal materials.
Description of the drawings：
Fig. 1 Eremias argus thin layers differentiate figure (1. Eremias argus control medicinal material, 2. Eremias argus)；
Fig. 2 skink thin layers differentiate figure (1. Eremias argus control medicinal material, 2. skink)；
Fig. 3 reunion of bone capsule lizards thin-layer chromatogram (1. Eremias argus control medicinal material, 2. reunion of bone capsule)
Specific implementation mode
The present invention is described further with the following Examples.
Embodiment 1
Hirudo leech from Lacertide Eremias argus Eremias argus Peters differentiate.
Eremias argus medicinal powder 1g is taken, adds ethyl acetate 20ml ultrasonic extractions 30 minutes, is filtered, filtrate is placed in water-bath
It is evaporated, residue adds ethyl acetate 1ml to make dissolving, as test solution, separately takes lizard control medicinal material powder 1g, adds ethyl acetate
20ml ultrasonic extractions 30 minutes, filtration, filtrate are placed in water-bath and are evaporated, and residue adds ethyl acetate 1ml to make dissolving, as a contrast medicine
Material solution.
According to thin-layered chromatography（General rule 0502）Experiment is drawn each 5 μ l of above two solution, is put respectively thin in same silica G
On laminate, with n-hexane-ethyl acetate（4:1）For solvent, be unfolded, take out, dry, spray with 10% ethanol solution of sulfuric acid,
105 DEG C to be heated to spot development clear.In test sample chromatography, on position corresponding with control medicinal material chromatography, identical face is shown
The principal spot of color.It is possible thereby to differentiate that medicinal material is Eremias argus.See Fig. 1.
Embodiment 2
Dry all discriminatings of internal organ are removed from Scincidae animal skink Eumeces chinensis (Gray).
Skink medicinal powder 1g is taken, adds ethyl acetate 20ml ultrasonic extractions 30 minutes, is filtered, filtrate, which is placed in water-bath, steams
Dry, residue adds ethyl acetate 1ml to make dissolving, as test solution, separately takes lizard control medicinal material powder 1g, adds ethyl acetate
20ml ultrasonic extractions 30 minutes, filtration, filtrate are placed in water-bath and are evaporated, and residue adds ethyl acetate 1ml to make dissolving, as a contrast medicine
Material solution.
According to thin-layered chromatography（General rule 0502）Experiment is drawn each 5 μ l of above two solution, is put respectively thin in same silica G
On laminate, with n-hexane-ethyl acetate（4:1）For solvent, be unfolded, take out, dry, spray with 10% ethanol solution of sulfuric acid,
105 DEG C to be heated to spot development clear.In test sample chromatography, on position corresponding with control medicinal material chromatography, identical face is shown
The principal spot of color.It is possible thereby to differentiate that medicinal material is lizard.See Fig. 2.
Embodiment 3
The discriminating of Cabrite medicinal materials in reunion of bone capsule.
Reunion of bone capsule 's content 2g is taken, adds ethyl acetate 20ml ultrasonic extractions 30 minutes, is filtered, filtrate is placed in water-bath
On be evaporated, residue adds ethyl acetate 1ml to make dissolving, as test solution.
Lizard control medicinal material solution is the same as embodiment 1.
According to thin-layered chromatography（General rule 0502）Experiment is drawn each 5 μ l of above two solution, is put respectively thin in same silica G
On laminate, with n-hexane-ethyl acetate（4:1）For solvent, be unfolded, take out, dry, spray with 10% ethanol solution of sulfuric acid,
105 DEG C to be heated to spot development clear.In test sample chromatography, on position corresponding with control medicinal material chromatography, identical face is shown
The principal spot of color.Contain Cabrite medicinal materials it is possible thereby to differentiate in reunion of bone capsule.See Fig. 3.
The experimental results showed that, the method for quality control feasibility of Chinese medicine lizard provided by the invention is strong, operation by above
Simplicity accurately can quickly differentiate the lizard in skink and reunion of bone capsule.Cabrite medicinal materials quality control side can effectively be made up
The blank of method has very strong practicability.
Claims (4)
1. a kind of method of quality control of Chinese medicine lizard, it is characterised in that：Include the following steps：
（1）Lizard powder 1g is taken, adds ethyl acetate 20ml ultrasonic extractions 30 minutes, is filtered, filtrate is placed in water-bath and is evaporated, residue
Ethyl acetate 1ml is added to make dissolving, as test solution；Lizard control medicinal material 1g separately is taken, is made in the same way of control medicinal material solution；
（2）Each 5-20 μ l of above two solution are drawn, are put respectively on same silica gel g thin-layer plate, with n-hexane-ethyl acetate
（4:1）For solvent, it is unfolded, takes out, dry, with 10% ethanol solution of sulfuric acid, it is clear to be heated to spot development at 105 DEG C, supplies for spray
In test product chromatography, on position corresponding with control medicinal material chromatography, the spot of same color is shown.
2. a kind of method of quality control of Chinese medicine lizard according to claim 1, it is characterised in that：
Step（1）The extraction solvent of lizard index components is ethyl acetate, chloroform, wherein being preferably ethyl acetate；
Step（1）In extracting mode be ultrasonic extraction 20min, 30min, wherein preferably be ultrasonic extraction 30min；
Step（2）In solvent be n-hexane-ethyl acetate（4:1）；
Step（2）In color developing agent be 10% ethanol solution of sulfuric acid, 10% phosphomolybdic acid ethyl alcohol, wherein preferably be 10% sulfuric acid ethyl alcohol
Solution；
Step（2）In colour temp be 105 DEG C heating develop the color.
3. according to a kind of method of quality control of Chinese medicine lizard of claim 1 to 2 any one of them, which is characterized in that described
Test medicine be Chinese medicine lizard and compound, Chinese patent drug etc. containing Chinese medicine lizard.
4. a kind of method of quality control of Chinese medicine lizard according to claim 3, which is characterized in that the test medicine
For skink, Eremias argus and reunion of bone capsule, reunion of bone piece etc..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710080187.4A CN108426973A (en) | 2017-02-15 | 2017-02-15 | The method of quality control of Chinese medicine lizard and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710080187.4A CN108426973A (en) | 2017-02-15 | 2017-02-15 | The method of quality control of Chinese medicine lizard and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108426973A true CN108426973A (en) | 2018-08-21 |
Family
ID=63155310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710080187.4A Pending CN108426973A (en) | 2017-02-15 | 2017-02-15 | The method of quality control of Chinese medicine lizard and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108426973A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210915A (en) * | 2007-12-24 | 2008-07-02 | 王志敏 | Cabrite medicinal materials discrimination method |
| CN104020247A (en) * | 2014-06-25 | 2014-09-03 | 南通永康检测技术有限公司 | Method for detecting quality of bone reunion tablets |
-
2017
- 2017-02-15 CN CN201710080187.4A patent/CN108426973A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210915A (en) * | 2007-12-24 | 2008-07-02 | 王志敏 | Cabrite medicinal materials discrimination method |
| CN104020247A (en) * | 2014-06-25 | 2014-09-03 | 南通永康检测技术有限公司 | Method for detecting quality of bone reunion tablets |
Non-Patent Citations (3)
| Title |
|---|
| PETER ZANI ET AL: "Characterization and Quantification of the Neutral Lipids in the Lizard Uta stansburiana stansburiana by HPTLC-Densitometry", 《 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES》 * |
| 胡丽华 等: "蛤蚧各部位药化成分及其毒性之初步分析", 《辽宁中医杂志》 * |
| 陈蔼 等: "对《中国药典》(2005年版一部)薄层色谱法中部分含苯、甲苯展开剂的改进", 《广东药学院学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102973619B (en) | Process for extracting indoline amide alkaloid from purslane and detection methods for indoline amide alkaloid | |
| Zangeneh et al. | Therapeutic effects of Glycyrrhiza glabra aqueous extract ointment on cutaneous wound healing in Sprague Dawley male rats | |
| CN104224813B (en) | Pharmaceutical composition as well as preparation method and application thereof | |
| CN105241996A (en) | Thin-layer chromatography identification method of carbonized herb processed product | |
| CN108426973A (en) | The method of quality control of Chinese medicine lizard and its application | |
| CN102106972B (en) | Medicament for treating empyrosis and scalding and acute skin bruise and contusion, preparation method and detection method | |
| CN106963797A (en) | A kind of method that extraction from folium artemisiae argyi prepares Chinese medicine antibacterial preparation | |
| CN103610766A (en) | Cold-dispelling cold capsule | |
| CN106896180A (en) | A kind of detection method of Bushen Tianjing oral liquid | |
| CN107353205B (en) | Ester compound in nigella glandulifera Freyn seeds as well as preparation method and application thereof | |
| CN104198644A (en) | Agilawood identifying method | |
| CN102361644B (en) | Anti dengue activity of cissampelos pareira extracts | |
| Nissi et al. | Development and characterization of Morinda tinctoria incorporated electrospun PHBV fiber mat for wound healing application | |
| CN103861078B (en) | A kind of snakes and scorpions analgesia liquor for treating rheumatism and preparation method thereof | |
| CN102662027B (en) | Thin-layer chromatography identification method of caulis tinosporae sinensis formula granules | |
| CN105983021A (en) | External-use medicinal composition as well as preparation method and applications thereof | |
| CN105738553B (en) | The thin-layered chromatography detection method of lophatherum gracile in a kind of qingwen baidu powder | |
| Omanakuttan et al. | Comparative Analysis of Maceration and Soxhlation for the Extraction and Preliminary Phytochemical Screening of the Roots of Cassia fistula L. | |
| CN1169873A (en) | Medicine for skin diseases and preparation thereof | |
| CN103558330A (en) | Thin-layer identification chromatography method of stomach-strengthening ulcer-curing preparation | |
| CN104154720A (en) | Application of freeze-drying technology in eucommia ulmoides drying | |
| CN114796196B (en) | External medicine composition for treating vascular vertigo and application thereof | |
| CN104352572B (en) | Glossy privet fruit processing method | |
| CN103592406B (en) | A kind of detection method of xianyu granule being used for the treatment of epilepsy clonus, child convulsion, facial spasm | |
| Vasenda et al. | Research of technological factors on the extraction process of bas from walnut membranes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180821 |