CN108426835A - It can be used for the agglutination test tube of instrument colorimetric - Google Patents
It can be used for the agglutination test tube of instrument colorimetric Download PDFInfo
- Publication number
- CN108426835A CN108426835A CN201810591994.7A CN201810591994A CN108426835A CN 108426835 A CN108426835 A CN 108426835A CN 201810591994 A CN201810591994 A CN 201810591994A CN 108426835 A CN108426835 A CN 108426835A
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- China
- Prior art keywords
- test tube
- cavity
- spherical cavity
- agglutination
- test
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 85
- 230000004520 agglutination Effects 0.000 title claims abstract description 36
- 208000002925 dental caries Diseases 0.000 claims abstract description 7
- 238000009738 saturating Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 10
- 238000002798 spectrophotometry method Methods 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 239000004744 fabric Substances 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 238000002834 transmittance Methods 0.000 description 4
- 206010006500 Brucellosis Diseases 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- IICCLYANAQEHCI-UHFFFAOYSA-N 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodospiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C(C(=C(Cl)C(Cl)=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 IICCLYANAQEHCI-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 229930187593 rose bengal Natural products 0.000 description 1
- 229940081623 rose bengal Drugs 0.000 description 1
- STRXNPAVPKGJQR-UHFFFAOYSA-N rose bengal A Natural products O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 STRXNPAVPKGJQR-UHFFFAOYSA-N 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses a kind of agglutination test tubes can be used for instrument colorimetric, its shape is positive cubic post shapes, and tube wall light transmission, there are two cavitys for test tube, one is spherical cavity, the other is square column type cavity, spherical cavity is located at the bottom position of test tube, and square column type cavity is located at the middle part of test tube to upper position, a circular open is arranged at the top of spherical cavity, the lower part of square column type cavity is funnel shaped, and bell mouth connects with the circular open, and two cavitys communicate.Spherical cavity can store deposit in test tube, prevent the liquid perturbation because of caused by mobile test tube from deposit being made to rise；Square column type cavity part is such as cuvette, therefore the test tube can use on spectrophotometer, and agglutination test can be more objective, accurate by spectrophotometry interpretation experimental result；Test result has a data foundation, and can long-term preservation, and overcome the having different views for generating due to naked eyes interpretation.
Description
Technical field
The present invention relates to a kind of glasswares of Biochemical Lab, and in particular to be a kind of cloth disease tube agglutination test
The middle agglutination test tube used.
Background technology
Cloth disease tube agglutination test (SAT) is a kind of stability, specificity, the higher detection method of sensibility, is always
Various countries are used as the conventional serological diagnostic method of diagnosis humans and animals brucellosis, and China is classified as animal brucellosis
The legal formal test of national standard of diagnosis.According to GB/T 18646-2002, in examining for animal brucellosis (hereinafter referred to as cloth disease)
In disconnected technology, " rose bengal precipitation test (RBPT), cow full milk ring test (MRT) are suitable for domestic animal cloth disease Field Screening
Experiment and the monitoring of dairy farm cloth disease and the Primary Screening Test for diagnosing lactating cow cloth disease；Tube agglutination test (SAT) and complement knot
Close the domestic animal that experiment (CFT) is suitable for diagnosing sheep kind, ox kind and the infection of pig kind cloth disease ".
However, all the time in cloth disease tube agglutination test method, the interpretation of test result is artificial naked eyes interpretation, i.e.,
It visually observes in test tube by the agglutination phenomenon of inspection serum, the relative amount of Serum Antibody is judged with this.It is this to rely on artificial meat
Eye interpretation certainly exists error, especially when agglutination phenomenon is less apparent, the working experience of laboratory technician, eyes color discrimination and
The difference meeting interpretation of eyesight goes out different results.Therefore, people attempt with instrument come interpretation, and a kind of best selection is with light splitting
Photometer does the liquid in agglutination test tube the measurement of light transmittance, and the data foundation of interpretation is obtained by light transmittance.But it is existing
Conventional agglutination test tube be in cylindrical type, cannot be used on spectrophotometer.If directly in the cuvette of spectrophotometer into
Then row agglutination test carries out determination of light transmittance, but since the flat-bottom structure of cuvette can be because of the rolling of liquid during interpretation
It moves and bottom agglutination precipitate is caused to rise, influence test result accuracy, or even cause serious error.If from completing to try
It is measured in the cuvette that the conventional agglutination test tube tested takes supernatant to be fitted into spectrophotometer, on the one hand increases experiment step
Suddenly, link and experiment equipment increase the probability that accidental error occurs；On the other hand it is to take liquid process brighter to liquid perturbation
It is aobvious, the uncertain bigger of test result.
Invention content
In view of this, the present invention proposes that a kind of agglutination test tube can be used for instrument colorimetric, this agglutination test tube can divide
It uses on light photometer, is measured by the light transmittance to liquid in test tube, provided for the accurate judgement of agglutination test result
Clear data foundation.
The technical scheme is that：A kind of agglutination test tube can be used for instrument colorimetric, which is characterized in that the shape of test tube
For positive cubic post shapes, tube wall light transmission, there are two cavitys for test tube, and one is spherical cavity, the other is square column type cavity, spherical
Cavity is located at the bottom position of test tube, and square column type cavity is located at the middle part of test tube to upper position, and the top of spherical cavity has
The lower part of circular open, square column type cavity is funnel shaped, and bell mouth connects with the circular open, and two cavitys communicate.
In the present invention, the thickness of the tube wall and be 1mm.
Further, the bore of the circular open is the 1/3~1/2 of spherical cavity diameter.
Further, the height of the test tube is 27mm, and the bore of a diameter of 4mm of spherical cavity, circular open are 2mm,
The height of funnel is 5~8mm.
Further, the center of the spherical cavity is located on the center line of the test tube, and the midpoint of circular open is located at examination
On the center line of pipe.
Further, the center line of test tube described in the center deviation of the spherical cavity, the edge of circular open is close to test tube
One side side wall.
The advantage of the invention is that：1, spherical cavity can store deposit in test tube, prevent from causing because of mobile test tube
Liquid perturbation so that deposit is risen, so as to reduce test error；2, it can be used on spectrophotometer, square column type chamber
Body portion is such as cuvette, and therefore, cloth disease tube agglutination test can carry out in the present invention, and can pass through spectrophotometry
Interpretation experimental result, it is more objective, accurate；3, test result has a data foundation, and can long-term preservation, and overcome because visually sentencing
Read and generate have different views.
Description of the drawings
Fig. 1 is the front perspective view of one embodiment of the invention.
Fig. 2 is the vertical view of embodiment illustrated in fig. 1.
Fig. 3 is A-A sectional views in Fig. 2.
Fig. 4 is the stereoscopic schematic diagram of embodiment illustrated in fig. 1.
Fig. 5 is the front perspective view of another embodiment of the present invention.
Fig. 6 is the vertical view of embodiment illustrated in fig. 5.
Fig. 7 is B-B sectional views in Fig. 6.
Fig. 8 is the stereoscopic schematic diagram of embodiment illustrated in fig. 5.
In figure：1 tube wall, 2. spherical cavities, 3. circular opens, 4. square column type cavities, 5. funnels, 6. bell mouths.
Specific implementation mode
The invention will be further described below in conjunction with the accompanying drawings.
As shown in Figures 1 to 8, the agglutination test tube of the present invention that can be used for instrument colorimetric, shape are positive tetragonal prism
Shape, upper end opening, 1 light transmission of tube wall, there are two cavitys for test tube, and one is spherical cavity 2, the other is square column type cavity 4, ball
Shape cavity 2 is located at the bottom position of test tube, and square column type cavity 4 is located at middle part to upper position, the top of spherical cavity 2 of test tube
There is a circular open 3, the lower part of square column type cavity 4 is funnel shaped, as Fig. 1 and funnel shown in fig. 55, bell mouth 6 and circle
Shape opening 3 connects, and spherical cavity 2 and square column type cavity 4 communicate.
In the present invention, the thickness of tube wall 1 and be 1mm.The bore of circular open 3 and the bore of bell mouth 6 are equal, are spherical
The 1/3~1/2 of 2 diameter of cavity.
In two embodiments as shown in the figure, the height of test tube is 27mm, a diameter of 4mm of spherical cavity 2, and circle is opened
The bore of mouth 3 and the bore of bell mouth 6 are 2mm, and the height of funnel 5 is 5~8mm.
In the present invention, the center of spherical cavity 2 can be located on the center line of test tube (as shown in Figures 1 to 4), can also be inclined
Center line from test tube (as shown in Fig. 5 to Fig. 8).When the center of spherical cavity 2 is located on the center line of test tube, circular open 3
Midpoint be located on the center line of test tube (as shown in Figure 1, Figure 3)；When on the center line of the center deviation test tube of spherical cavity 2,
One side side wall (as shown in Figure 5, Figure 7) of the edge of circular open 3 close to test tube.
In the present invention, spherical cavity 2 can store deposit in test tube, prevent the liquid perturbation because of caused by mobile test tube
Deposit is set to rise；Square column type cavity 4 can be used such as cuvette on spectrophotometer.Therefore, cloth disease tube agglutination is tried
Test to carry out in the present invention, and the light absorption value of liquid in square column type cavity 4 can be read by spectrophotometry, to sentence
Read experimental result.
The material of the present invention can be quartzy, glass, can also be transparent plastic.The low cost of transparent plastic,
Test tube can be used as disposable.
The present invention illustrates in agglutination test, on spectrophotometer using and with result judgement：
1, by the present invention per tetrad, and 1-4 test tubes are marked successively；
2, in 1-4 test tubes, respectively with specify dilution two-fold dilution serum (such as 1: 50,1: 100,1: 200,1:
400) agglutination test is carried out with settable volume agglutination antigen to react；
3, it stands.In the test tube of antibody positive sample, since antibody and the agglutination antigen of different dilutions can be aggregated
The compound agglutinator of antibody antigen of different number is reacted and generates, and extension at any time gradually deposits, and is come together in by funnel 5
It is stored in bottom hemispheric cavity 2, different limpid degree are also gradually presented in square column type cavity 4 and show not for simultaneous reactions liquid
Same extinction characteristic.
4, after reaching and feel as defined in the legal tube agglutination test of country and do the time (usually 24-48 hours), will more than
Each group is aggregated test tube and carries out spectrophotometry comparison interpretation into spectrophotometer and standard opacity tube, and records absorbance value.
5, compared with the light absorption value of prescribed concentration standard opacity tube, light absorption value is more than the agglutination test tube of specified value opacity tube
Test result is determined as feminine gender；The agglutination test tubes result judgement that light absorption value is less than specified value opacity tube is the positive.
Claims (6)
1. a kind of agglutination test tube can be used for instrument colorimetric, it is characterised in that：The shape of test tube is positive cubic post shapes, and tube wall is saturating
Light, there are two cavitys for test tube, and one is spherical cavity, the other is square column type cavity, spherical cavity is located at the bottom position of test tube
It sets, square column type cavity is located at the middle part of test tube to upper position, and a circular open is arranged at the top of spherical cavity, square column type cavity
Lower part is funnel shaped, and bell mouth connects with the circular open, and two cavitys communicate.
2. the agglutination test tube described in accordance with the claim 1 that can be used for instrument colorimetric, it is characterised in that：The thickness of the tube wall and
For 1mm.
3. the agglutination test tube described in accordance with the claim 1 that can be used for instrument colorimetric, it is characterised in that：The mouth of the circular open
Diameter is the 1/3~1/2 of spherical cavity diameter.
4. the agglutination test tube described in accordance with the claim 1 that can be used for instrument colorimetric, it is characterised in that：The height of the test tube is
The bore of 27mm, a diameter of 4mm of spherical cavity, circular open are 2mm, and the height of funnel is 5~8mm.
5. the agglutination test tube described in accordance with the claim 1 that can be used for instrument colorimetric, it is characterised in that：In the spherical cavity
The heart is located on the center line of the test tube, and the midpoint of circular open is located on the center line of test tube.
6. the agglutination test tube described in accordance with the claim 1 that can be used for instrument colorimetric, it is characterised in that：In the spherical cavity
The heart deviates the center line of the test tube, one side side wall of the edge of circular open close to test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810591994.7A CN108426835A (en) | 2018-05-30 | 2018-05-30 | It can be used for the agglutination test tube of instrument colorimetric |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810591994.7A CN108426835A (en) | 2018-05-30 | 2018-05-30 | It can be used for the agglutination test tube of instrument colorimetric |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108426835A true CN108426835A (en) | 2018-08-21 |
Family
ID=63164822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810591994.7A Pending CN108426835A (en) | 2018-05-30 | 2018-05-30 | It can be used for the agglutination test tube of instrument colorimetric |
Country Status (1)
| Country | Link |
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| CN (1) | CN108426835A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4511349A (en) * | 1982-07-06 | 1985-04-16 | Beckman Instruments, Inc. | Ultracentrifuge tube with multiple chambers |
| US9360433B1 (en) * | 2013-05-21 | 2016-06-07 | Indevr, Inc. | Detection of agglutination by optical density measurement |
| WO2016204533A1 (en) * | 2015-06-16 | 2016-12-22 | (주)타스컴 | Biological material measuring instrument |
| CN107003230A (en) * | 2014-09-29 | 2017-08-01 | Bd科斯特公司 | Equipment and its cuvette for the fluid sample of optical check small size |
| CN107923838A (en) * | 2015-06-16 | 2018-04-17 | 泰斯科有限公司 | Biomaterial determining instrument |
| CN208187962U (en) * | 2018-05-30 | 2018-12-04 | 韩永刚 | It can be used for the agglutination test tube of instrument colorimetric |
-
2018
- 2018-05-30 CN CN201810591994.7A patent/CN108426835A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4511349A (en) * | 1982-07-06 | 1985-04-16 | Beckman Instruments, Inc. | Ultracentrifuge tube with multiple chambers |
| US9360433B1 (en) * | 2013-05-21 | 2016-06-07 | Indevr, Inc. | Detection of agglutination by optical density measurement |
| CN107003230A (en) * | 2014-09-29 | 2017-08-01 | Bd科斯特公司 | Equipment and its cuvette for the fluid sample of optical check small size |
| WO2016204533A1 (en) * | 2015-06-16 | 2016-12-22 | (주)타스컴 | Biological material measuring instrument |
| CN107923838A (en) * | 2015-06-16 | 2018-04-17 | 泰斯科有限公司 | Biomaterial determining instrument |
| CN208187962U (en) * | 2018-05-30 | 2018-12-04 | 韩永刚 | It can be used for the agglutination test tube of instrument colorimetric |
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