CN109425588A - Hand-held device, method and the computer readable storage medium of rapid identification yellow twig - Google Patents
Hand-held device, method and the computer readable storage medium of rapid identification yellow twig Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000012545 processing Methods 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 15
- 238000003384 imaging method Methods 0.000 claims abstract description 8
- 241000207199 Citrus Species 0.000 claims description 18
- 235000020971 citrus fruits Nutrition 0.000 claims description 18
- 238000004611 spectroscopical analysis Methods 0.000 claims description 17
- 238000012937 correction Methods 0.000 claims description 14
- 239000011159 matrix material Substances 0.000 claims description 14
- 238000001228 spectrum Methods 0.000 claims description 14
- 238000004445 quantitative analysis Methods 0.000 claims description 8
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- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
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- 238000003745 diagnosis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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Abstract
The invention discloses a kind of hand-held devices of rapid identification yellow twig, including handle, the body of a gun and self-test on-gauge plate, the handle is connected with the body of a gun, the body of a gun is equipped with trigger, front cover and driving rod, the trigger is connected with the front cover by the driving rod, the fixed sample to be tested of the front cover, the self-test on-gauge plate fixed standard sample, for being corrected to detection model；The body of a gun is also equipped with light source, the first convergent lens, collimation lens, grating, imaging len, dmd chip, the second convergent lens, single point detector, signal processing system and display screen, the single point detector is connected with the signal processing system, and the signal processing system is connected with the display.The invention also discloses the methods and computer readable storage medium of a kind of rapid identification yellow twig.The present invention its can quickly, accurately detect yellow twig, and do not need full-time staff, environmental protection.
Description
Technical field
The present invention relates to yellow twig recognition techniques, and in particular to hand-held device, method and the calculating of rapid identification yellow twig
Machine readable storage medium storing program for executing.
Background technique
Currently, yellow twig is to infect other positions rapidly as caused by the gramnegative bacterium for parasitizing bast.
It is mainly propagated in a manner of wood louse, Semen Cuscutae and grafting etc. yellow twig, and transmission speed is fast, and a fruit tree infection can cause big
Piece orchard is fallen into disuse, extinction of species.Yellow twig can contain yellow twig to being destructive brought by Citrus Industry in time
Fast propagation, promptly cutting off propagating source is particularly important.It is real-time fluorescence that a kind of common yellow twig, which makes a definite diagnosis detection method,
Quantitative PCR (Quantitative Real-time PCR) is one kind in DNA amplification reaction, is surveyed with fluorescent chemical each
The method of product total amount after polymerase chain reaction (PCR) circulation.Using its CT value as judging Huanglong in real-time fluorescence quantitative PCR
The foundation of sick infected plant.Wherein CT value (Cycle threshold, cycle threshold) refers to: the fluorescence letter in each reaction tube
Recurring number experienced when number reaching given threshold.Wherein the logarithm of CT value content and starting copy number is linear, that is, rises
Beginning copy number is bigger, and CT value is with regard to smaller.It is to judge boundary when being wherein 32 with CT value most of in yellow twig, that is, being greater than 32 is
Non- yellow twig is yellow twig less than 32.Real-time fluorescence quantitative PCR is a kind of traditional yellow twig detection means, detection week
Time phase is long, substantially needs one week or so；Testing cost is expensive, and third party testing agency is sent once to need 100~400 yuan to differ, because
The most usage scenario of this this method is laboratory.
But existing technology has the following deficiencies:
(1) civil diagnosis needs veteran expert or technical staff, needs diagnostic experiences abundant, subjective impact because
Element is big, reproducibility is poor；
(2) DNA reagent extracts box reagent and not can be recycled, and expendable is big, recyclability and the feature of environmental protection are poor, and detects
As a result non-recordable.
Summary of the invention
For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide the hand-held dresses of rapid identification yellow twig
Set, can quickly, accurately detect yellow twig, and do not need full-time staff, environmental protection.
The second object of the present invention is to provide the method for rapid identification yellow twig, can quickly, accurately detect Huanglong
Disease, and full-time staff is not needed, environmental protection.
The third object of the present invention is to provide computer readable storage medium, can quickly, accurately detect yellow twig,
And full-time staff is not needed, environmental protection.
An object of the present invention adopts the following technical scheme that realization:
A kind of hand-held device of rapid identification yellow twig, comprising: handle, the body of a gun and self-test on-gauge plate, the handle and institute
Body of a gun connection is stated, the body of a gun is equipped with trigger, front cover and driving rod, and the trigger and the front cover are connected by the driving rod
It connects, the fixed sample to be tested of the front cover, the self-test on-gauge plate fixed standard sample, for being corrected to detection model；Institute
State the body of a gun be also equipped with light source, the first convergent lens, collimation lens, grating, imaging len, dmd chip, the second convergent lens,
Single point detector, signal processing system and display screen, the single point detector are connected with the signal processing system, the signal
Processing system is connected with the display；
The illumination of light source, which is mapped on sample to be tested or standard sample, reflects, successively by first convergent lens, collimation
Lens, grating and imaging len post-concentration form the picture of the slit of different wave length, through the dmd chip on the dmd chip
It is focused on the single point detector after carrying out wavelength gating by second convergent lens, the single point detector will be received
Optical signal is sent to the signal processing system, the signal processing system to after the optical signal prosessing by the display screen into
Row display.
Further, the single point detector is single-point photodiode.
Further, the body of a gun is equipped with detection key, and the detection key is connect with the light source.
Further, reference plate is installed on the front cover, the body of a gun is equipped with reference key corresponding with reference plate,
The reference key is connect with the light source.
The second object of the present invention adopts the following technical scheme that realization:
A kind of method of rapid identification yellow twig, comprising the following steps:
Determination step: acquiring the CT value content in CT value contained by multiple sampled points difference in each sample to be tested,
And the CT value concentration matrix for forming the sample to be tested is denoted as Y, Y={ y1,y2,y3,...yn}；The sample to be tested includes yellow twig
The blade of the blade of citrus and non-yellow twig citrus；Only one CT value of the different sampled point of each sample to be tested；
Acquisition step: the spectroscopic data of multiple sampled points of each sample to be tested is acquired, and forms spectrum data matrix
X is denoted as,
Modeling procedure: according to the CT value concentration matrix of sample to be tested and spectrum data matrix to preset quantitative analysis method shape
It is B=(X at the quantitative correction relationship between CT value and spectroscopic dataTX)-1XTY, wherein B is quantitative correction relationship；
Prediction steps: inputting the spectroscopic data of sample to be tested, according to quantitative correction relationship and formula: YIt is unknown=X*B is somebody's turn to do
The CT predicted value of sample to be tested, YIt is unknownFor CT predicted value；
Judgment step: judging whether the CT value of the sample to be tested is lower than preset reference value, if so, the sample to be tested is not
Suffer from yellow twig, otherwise, sample to be tested suffers from yellow twig.
Further, in modeling procedure, presetting quantitative analysis method is Partial Least Squares.
Further, preset reference value described in judgment step is 32.
The third object of the present invention adopts the following technical scheme that realization:
A kind of computer readable storage medium, is stored thereon with computer program, and the computer program is by a signal processing
Device realizes the judgment step of two the methods of the object of the invention when executing.
Compared with prior art, the beneficial effects of the present invention are:
(1) by infrared detection, full-time technical staff is not needed to operate, adaptable, applicability is wide；
(2) it can persistently be used for multiple times, it is more environmentally-friendly, and be convenient for carrying；
(3) it can fast and accurately detect whether to suffer from yellow twig.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the hand-held device of rapid identification yellow twig of the invention；
Fig. 2 is the cross-sectional view of Fig. 1；
Fig. 3 is the flow chart of the method for rapid identification yellow twig of the invention；
Fig. 4 is the spectrogram of 268 samples to be tested；
Fig. 5 is the comparison diagram for modeling sample to be tested measured value and near-infrared predicted value；
Fig. 6 is the comparison diagram of unknown sample to be tested measured value and near-infrared predicted value.
In figure: 10, handle；11, battery；20, the body of a gun；21, light source；22, the first convergent lens；23, collimation lens；24,
Grating；25, imaging len；26, dmd chip；27, the second convergent lens；28, single point detector；30, trigger；31, driving rod；
40, front cover；41, reference plate；50, sample to be tested；60, signal processing system；70, display screen；80, key is detected；90, reference is pressed
Key；100, self-test on-gauge plate.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention, it should be noted that not
Under the premise of conflicting, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination
Example.
As illustrated in fig. 1 and 2, the hand-held device of the invention first for showing a kind of rapid identification yellow twig, including handle
10 and fuselage 20, handle 10 be fixedly connected with the body of a gun 20, battery 11 is installed inside handle 10, the body of a gun 20 be equipped with trigger 30,
Driving rod 31 and front cover 40, trigger 30 and front cover 40 are connected by driving rod 31, and reference plate 41, trigger 30 are equipped on front cover 40
For controlling the opening and closing of front cover 40, the body of a gun 20 is also equipped with light source 21, the first convergent lens 22, collimation lens 23, light
Grid 24, imaging len 25, dmd chip 26, the second convergent lens 27, single point detector 28, system signal processing 60 and display screen
70, single point detector 28 and signal processing system 60 connect, and signal processing system 60 and display 70 connect, light source 21, DMD core
Piece 26, single point detector 28, signal processing system 60, display screen 70 are connect with battery 11 by conducting wire, and single point detector 28 is
Single-point photodiode, the body of a gun 20 are also equipped with detection key 80, and detection key 80 is connect with light source 21, and control light source 21 is beaten
It is open and close.
The light that light source 21 issues is reflected by sample to be tested 50, is assembled by the first convergent lens 22, by collimation lens
It is collimated after 23, dispersion is band after being irradiated to grating 24, is incident on DMD according to wavelength order convergence after imaging len 25
On chip 26, dmd chip 26 is focused on single point detector 28 after carrying out wavelength gating by the second convergent lens 17, single-point detection
Received optical signal is sent to signal processing system 60 by device 28, signal processing system 60 to after optical signal prosessing by display screen 70
It is shown.
In use, opening front cover 40 by trigger 30, trigger 30 is decontroled after sample to be tested 50 is placed, front cover 40 will at this time
Sample to be tested 50 is fixed, opens light source 21 by detection key 80 and is detected, and testing result is directly displayed on display screen 70,
Facilitate testing staff to check, front cover 40 is opened by trigger 30 after detection, takes out sample to be tested 50, it is convenient and efficient.
As further embodiment, it is fixedly installed with reference plate 41 on the inside of front cover 40, is checked and approved for hand-held device,
It when being calibrated using 41 hand-held device of reference plate, is realized by starting reference key 90, process and detection key 80
It operates similar.In addition, the hand-held device further includes certainly in order to be corrected to detection model (step S1-S4 in following methods)
Calibration plate 100 is examined, fixed standard sample is used for, the process of correction is also to be realized by detection key 80, i.e., works as standard sample
It is detected as sample to be tested, self-test calibration plate 100 replaces front cover 40 to be set in optical path, then will test and is sent to inspection in result
Platform is surveyed, detection platform is allowed to differentiate the change rate of hand-held device by certain algorithm and carries out model correction.
The appearance of handheld device is pistol type, detects and is convenient for carrying convenient for operator；The cost of equipment is reduced,
The MEMS micro-electromechanical technology improved using minimum；Personnel check as a result, equipment belt display screen for ease of operation, when detection to
When sample is yellow twig, yellow twig is shown, and drip drop prompting sound；When detecting sample to be tested is non-yellow twig, non-Huang is shown
Imperial disease；When the sample to be tested of detection is non-citrus blade, display sample to be tested is abnormal.For the epidemic prevention and control of yellow twig,
GPS positioning module built in equipment and memory module, to the detection geographical location of each blade sample to be tested, testing result, detection
Spectrum is stored, and is used for subsequent analysis.
As shown in figure 3, the present invention also provides a kind of methods of rapid identification yellow twig, comprising the following steps:
S1: acquiring the CT value content in CT value contained by multiple sampled points difference in each sample to be tested, and ps is each
Only one CT value of the different sampled point of a sample to be tested, and the CT value concentration matrix for forming the sample to be tested is denoted as Y, Y=
{y1,y2,y3,...yn}；The sample to be tested includes the blade of yellow twig citrus and the blade of non-yellow twig citrus；
In order to which what a good model established, it is necessary first to be collected to sample to be tested, collection has each of yellow twig
The blade of class symptom includes that yellow, mottled, Lutao, blade are small etc., non-yellow twig sample to be tested is for example mottled, nutritional deficiency, without obvious disease
Shape etc..Because non-yellow twig there are some symptoms similar with yellow twig, therefore the qualitative of near-infrared is established with non-yellow twig and yellow twig
Erroneous judgement can be easily caused when model, and uses the Quantitative Analysis Model of CT value foundation for a certain degree, as caused by yellow twig
The blade CT value contents of the symptoms such as yellow differ markedly from the symptoms such as the yellow of non-yellow twig, can be big therefore in prediction
The accuracy rate of big raising model, to reduce the probability of erroneous judgement.
In this step, representative citrus sample to be tested is selected, the near infrared spectrum of sample to be tested is tested, in the blade
Sample to be tested in several optimal acquisition positions are obtained according to experiment, that is, multiple sampled points.This step is in reality
It can or reversed order synchronous with S2 progress in the operation of border.
S2: acquiring the spectroscopic data of multiple sampled points of each sample to be tested, and form spectrum data matrix and be denoted as X,
In this step, it using a fluorescent quantitative PCR detector device and primer of the same race, detects and feels in citrus sample to be tested
It catches an illness bacterium situation, obtains each of which corresponding CT value of sample to be tested difference sampled point, form CT value concentration matrix.Here spectrum
The sampled point of data, the front and back that can be in sample to be tested are set separately.Blade additionally, due to illness and do not suffer from
The blade of disease, spectroscopic data collected can be divided into the spectroscopic data and non-yellow twig Citrus leaf of yellow twig Citrus leaf
Spectroscopic data.
S3: CT value is formed with default quantitative analysis method according to the CT value concentration matrix of sample to be tested and spectrum data matrix
Quantitative correction relationship between spectroscopic data is B=(XTX)-1XTY, wherein B is quantitative correction relationship；Default quantitative analysis
Method is least square method；
According to the CT value of sample to be tested, the front spectrum of the blade of yellow twig citrus, yellow twig citrus blade the back side
Spectrum, the front spectrum of the blade of non-yellow twig citrus, non-yellow twig citrus blade back side spectrum with preset quantitative analysis
Method establishes quantitative model；Using predicted root mean square error (RMSEC), unknown sample to be tested predicted root mean square error (RMSEP),
Related coefficient (RP) evaluates the error and correlation between near-infrared predicted value and measured value respectively, and formula is as follows:
Wherein,CiThe predicted value and measured value of respectively i-th prediction sample to be tested；CAVERespectively predicted value
With the average value of measured value；M is the number for predicting sample to be tested；The smaller precision for illustrating model of RMSEC and RMSEP value is higher,
RPValue is bigger, and the correlation for illustrating predicted value and measured value is higher.
In order to allow model to be more applicable in field conditions, equipment all is held for experimenter and acquires spectroscopic data.Acquisition
Spectroscopic data is divided into the leaf base of the leaf base of face of blade, middle part and tail portion and vacuum side of blade, middle part and tail portion.The mesh
The CT value model difference for predominantly distinguishing front and back, that is, establish model 1: positive spectroscopic data and CT value are established quantitatively
Model；Model 2: the spectroscopic data and CT value at the back side establish quantitative model.Because the chlorophyll content of material of front and back is different
Sample, therefore its spectrum has Light Difference, the purpose for establishing 2 models is to compare the CT value of unknown sample to be tested front and back
The acquisition of difference, the prediction for illustrating CT value content and blade different parts is without influence.
S4: inputting the spectroscopic data of sample to be tested, according to the quantitative correction relationship and formula: YIt is unknown=X*B obtain this to
The CT predicted value of sample, YIt is unknownFor CT predicted value；
S5: judging whether the CT value of the sample to be tested is lower than preset reference value, if so, the sample to be tested is not suffering from Huanglong
Disease, otherwise, sample to be tested suffer from yellow twig.Preset reference value is preferably 32, CT<32, yellow twig, CT>32, non-yellow twig.Certainly
Certain change occurs because different primers will lead to the critical value, therefore complete for the detection critical value of unknown sample to be tested
It is executed according to the value in step S2.
In the following, being further detailed with an embodiment:
Nir instrument is quickly detected as detecting instrument using NLD-HL10 yellow twig, tests Citrus Cultivars: sugar orange, detection
Index: CT value.The instrument wave-length coverage is 950-1650nm, is spaced 1nm, totally 701 wavelength.Specific step is as follows:
S1,268 sugar orange samples to be tested are tested according in S1, have obtained spectrum data matrix and CT value,
Middle table 1 and Fig. 4 are respectively the analysis of CT Data-Statistics and spectrogram of 268 Citrus shatangju samples to be tested.
The analysis of 1 sugar orange sample to be tested CT Data-Statistics of table
S2, the analysis model that CT value is established using Partial Least Squares, modeling result are as follows:
2 Partial Least Squares modeling result of table summarizes
S3, use Partial Least Squares establish the factor be 18 when, RMSEC minimum.It is calculated not according to correction relationship formula
Know the CT predicted value of sample to be tested.The spectroscopic data for scanning unknown sample to be tested calculates unknown to be measured according to correction relationship formula B
The CT value of sample.The prediction result of unknown sample to be tested is summarized as follows:
The prediction result of the unknown sample to be tested of table 3 summarizes
S4, according to CT value above and CT value tables of critical values, in conjunction with Fig. 5 and Fig. 6, it is known that 119 unknown samples to be tested
Prediction result are as follows: 42 are yellow twig sample to be tested, and 77 are non-yellow twig sample to be tested.The wherein result of measured value are as follows: 30
A yellow twig sample to be tested, 89 are non-yellow twig sample to be tested.Compare known to data above: total accuracy rate is 88%.
The abscissa of Fig. 5 and Fig. 6 is measured value, and ordinate indicates predicted value.
The present invention can also provide a kind of computer readable storage medium, be stored thereon with computer program, the computer
Step S5 is realized when program is executed by a signal processor.
The above embodiment is only the preferred embodiment of the present invention, and the range of invention protection cannot be limited with this, this
The variation and replacement for any unsubstantiality that the technical staff in field is done on the basis of the present invention belong to the present invention and are wanted
Seek the range of protection.
Claims (8)
1. a kind of hand-held device of rapid identification yellow twig, characterized by comprising: handle, the body of a gun and self-test on-gauge plate, it is described
Handle is connected with the body of a gun, and the body of a gun is equipped with trigger, front cover and driving rod, and the trigger and the front cover pass through described
Driving rod connection, the fixed sample to be tested of the front cover, the self-test on-gauge plate fixed standard sample, for being carried out to detection model
Correction；The body of a gun is also equipped with light source, the first convergent lens, collimation lens, grating, imaging len, dmd chip, the second meeting
Poly- lens, single point detector, signal processing system and display screen, the single point detector are connected with the signal processing system,
The signal processing system is connected with the display；
The illumination of light source, which is mapped on sample to be tested or standard sample, reflects, successively by first convergent lens, collimation lens,
Grating and imaging len post-concentration form the picture of the slit of different wave length on the dmd chip, carry out through the dmd chip
It is focused on the single point detector after wavelength gating by second convergent lens, the single point detector believes received light
Number it is sent to the signal processing system, the signal processing system is shown to after the optical signal prosessing by the display screen
Show.
2. hand-held device as described in claim 1, it is characterised in that: the single point detector is single-point photodiode.
3. hand-held device as described in claim 1, it is characterised in that: the body of a gun is equipped with detection key, and the detection is pressed
Key is connect with the light source.
4. hand-held device as described in claim 1, it is characterised in that: be equipped with reference plate, the body of a gun peace on the front cover
Equipped with reference key corresponding with reference plate, the reference key is connect with the light source.
5. a kind of method of rapid identification yellow twig, it is characterised in that comprising steps of
Determination step: the CT value content in CT value contained by multiple sampled points difference in each sample to be tested, and shape are acquired
Y, Y={ y are denoted as at the CT value concentration matrix of the sample to be tested1,y2,y3,...yn}；The sample to be tested includes yellow twig citrus
Blade and non-yellow twig citrus blade；Only one CT value of the different sampled point of each sample to be tested；
Acquisition step: the spectroscopic data of multiple sampled points of each sample to be tested is acquired, and forms spectrum data matrix and is denoted as
X,
Modeling procedure: CT is formed with default quantitative analysis method according to the CT value concentration matrix of sample to be tested and spectrum data matrix
Quantitative correction relationship between value and spectroscopic data is B=(XTX)-1XTY, wherein B is quantitative correction relationship；
Prediction steps: inputting the spectroscopic data of sample to be tested, according to quantitative correction relationship and formula: YIt is unknown=that X*B obtains this is to be measured
The CT predicted value of sample, YIt is unknownFor CT predicted value；
Judgment step: judging whether the CT value of the sample to be tested is lower than preset reference value, if so, the sample to be tested is not suffering from Huang
Imperial disease, otherwise, sample to be tested suffers from yellow twig.
6. method as claimed in claim 5, it is characterised in that: in modeling procedure, presetting quantitative analysis method is partially minimum two
Multiplication.
7. method as claimed in claim 5, it is characterised in that: preset reference value described in judgment step is 32.
8. a kind of computer readable storage medium, is stored thereon with computer program, it is characterised in that: the computer program is by one
The judgment step such as any one of claim 5-7 the method is realized when signal processor executes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710763407.3A CN109425588B (en) | 2017-08-30 | 2017-08-30 | Handheld device, method and computer-readable storage medium for quickly distinguishing huanglongbing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710763407.3A CN109425588B (en) | 2017-08-30 | 2017-08-30 | Handheld device, method and computer-readable storage medium for quickly distinguishing huanglongbing |
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| Publication Number | Publication Date |
|---|---|
| CN109425588A true CN109425588A (en) | 2019-03-05 |
| CN109425588B CN109425588B (en) | 2022-01-11 |
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| WO2024037674A1 (en) * | 2022-08-17 | 2024-02-22 | Universidad Simón Bolívar | Device for detecting hlb disease |
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