CN108424832B - Device with tissue fine-crushing device - Google Patents
Device with tissue fine-crushing device Download PDFInfo
- Publication number
- CN108424832B CN108424832B CN201710080916.6A CN201710080916A CN108424832B CN 108424832 B CN108424832 B CN 108424832B CN 201710080916 A CN201710080916 A CN 201710080916A CN 108424832 B CN108424832 B CN 108424832B
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- China
- Prior art keywords
- assembly
- motor
- filling
- tissue
- rotating plate
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- 230000006698 induction Effects 0.000 claims description 3
- 239000000945 filler Substances 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000001574 biopsy Methods 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Abstract
The invention provides a tissue fine crushing device and equipment with the same. The filling assembly comprises a hollow cylinder and a piston rod which can movably penetrate through the hollow cylinder, and one end of the piston rod is abutted against the motor assembly, so that the filling assembly is connected with the motor assembly. The filter screen is arranged at one end of the hollow cylinder body far away from the motor component. The guide tube is arranged at one side of the motor component and the filling component, wherein the guide tube comprises a nozzle arranged at one end of the guide tube and is adjacent to one end of the filter screen far away from the filling component. The cutting assembly is adjacent to the screen. The flow guide piece is arranged at one end of the hollow cylinder body, which is adjacent to the filter screen. The invention also provides equipment with the tissue fine crushing device, so that the effects of saving labor and time and unifying the tissue fine crushing degree are achieved.
Description
Technical Field
The invention relates to an automatic device, in particular to a device with a tissue fine crushing device.
Background
In recent years, the technology of extracting primary cell culture has been developed and well-established, and the continuous culture of primary cells has been applied to various fields, so that the extraction of primary cells is now urgently required to be manufactured in large quantities and unified in operation specifications.
In the prior art, since the extraction of primary cells involves a plurality of steps, the step of finely crushing the tissue is the most important step, and in addition to the fact that the separation must be completed in a short time, the extraction must be prevented from being contaminated from the outside. Therefore, the current step of finely dividing the tissue depends only on human operation, and the proficiency and care of the operator and the size of the divided tissue will affect the success and efficiency of the subsequent extraction of primary cells. Once a large amount of primary cells are extracted, the operation is performed by the same operator, which increases the operation time of fine tissue crushing, and once the pollution condition occurs in the middle, the cells are often discovered and cannot be recovered until the final culture stage.
In view of the above, there is a need in the art for an improved fine crushing device that can save manpower and reduce pollution.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the present invention provides a tissue crushing device and an apparatus having the same, so as to achieve the effects of saving labor and time and unifying the degree of tissue crushing.
To achieve the above objects, the present invention provides a tissue crushing device, which comprises a motor assembly, a filling assembly, a filter, a conduit, a cutting assembly and a flow guiding member. The filling assembly comprises a hollow cylinder and a piston rod; the piston rod can movably penetrate through the hollow cylinder, one end of the piston rod is abutted against the motor component, and the filling component is connected with the motor component. The filter screen is arranged at one end of the hollow cylinder body of the filling assembly, which is far away from the motor assembly. The guide pipe is arranged at one side of the motor component and the filling component, wherein the guide pipe comprises a nozzle which is arranged at one end of the guide pipe and is adjacent to one end of the filter screen far away from the filling component. The cutting assembly is adjacent to the screen. The flow guide piece is arranged at one end of the hollow cylinder of the filling assembly, which is opposite to the filter screen.
Preferably, the motor assembly is a stepping motor, a servo motor or an induction motor.
Preferably, the filter screen has a plurality of holes, and the filter screen is adjacent to one end of the filling assembly and forms a conical protrusion between the holes.
Preferably, the cutting assembly has a reciprocating blade and a first motor connected to the blade, and the blade is adjacent to an end of the strainer remote from the packing assembly.
Preferably, the cutting assembly is an ultrasonic blade set (ultrasonic instruments for tissue biopsy slicer) for tissue slicing.
Preferably, the cutting assembly is a laser cutter set.
The invention also provides equipment with the tissue fine crushing device, which comprises the tissue fine crushing device and a frame body. The frame body comprises a base, two plate bodies and a rotating plate. The plate bodies are L-shaped, and one end of each plate body is respectively connected with two sides of the base. The rotating plate can be rotatably arranged between the plate bodies and is far away from one end of the base; the rotating plate is provided with a second motor arranged on one side of the rotating plate; the tissue fine crushing device is arranged on the rotating plate.
Preferably, the rotating plate is provided with a spring locking device, one end of the spring locking device is fixedly arranged on one surface of the rotating plate, and the other end of the spring locking device can movably abut against the hollow cylinder of the filling component of the tissue fine crushing device by elasticity.
Preferably, the conduit is disposed on a side of the rotating plate away from the filling assembly.
Preferably, the cutting assembly is disposed on a surface of the rotating plate away from the filling assembly.
The tissue fine crushing device has the advantages that one end of the piston rod is abutted against the motor component, so that tissues in the hollow cylinder are extruded and separated through the holes; the filter screen is adjacent to one end of the filling assembly, and conical bulges are formed among the holes, so that tissues can be conveniently separated, squeezed into the holes, tissues at one end of the filter screen, which is far away from the filling assembly, are exposed, and then the exposed tissues are cut up by the cutting assembly. After the buffer solution contained in the conduit is sprayed out through the nozzle, the tissues are easy to separate. The cut tissues are guided along the guide member in sequence by the guide member for collection. In addition, the equipment with the tissue fine crushing device can be movably pulled through one end of the elastic locking device, which is abutted against the filling assembly, so that the filling assembly is easy to detach and load from the tissue fine crushing device. The rotating plate is provided with an electric motor for assisting in overturning, and the tissue fine crushing device can automatically perform tissue fine crushing separation so as to achieve the effects of saving manpower and time and unifying tissue fine crushing degree.
Drawings
The drawings are only for purposes of illustrating and explaining the present invention and are not to be construed as limiting the scope of the present invention. Wherein:
fig. 1 is a perspective view of a tissue mincing device according to the invention.
Fig. 2 is a partially exploded view of the tissue mincing device of the present invention.
Fig. 3 is a perspective view of a screen of the tissue disruption device of the present invention.
Fig. 4 is a cross-sectional view of a screen of another preferred embodiment of the tissue mincing device of the invention.
Fig. 5 is a perspective view of the apparatus of the present invention having tissue disruption means.
Fig. 6 is an exploded view of the apparatus of the present invention having tissue disruption means.
Detailed Description
The technical means adopted by the invention to achieve the purpose are further described below by combining the drawings and the preferred embodiments of the invention.
Referring to fig. 1 and 2, a tissue fine-crushing device according to the present invention includes a motor assembly 10, a filling assembly 20, a filter 30, a conduit 40, a cutting assembly 50, and a guide member 60.
The motor assembly 10 is a stepper motor, a servo motor or an induction motor.
The filling assembly 20 is connected to the motor assembly 10, the filling assembly 20 includes a hollow cylinder 21 and a piston rod 22; the piston rod 22 is movably disposed through the hollow cylinder 21, and one end of the piston rod 22 abuts against the motor assembly 10.
Referring to fig. 2 to 4, the filter 30 is disposed at an end of the hollow cylinder 21 of the filling assembly 20 away from the motor assembly 10. The screen 30 has a plurality of holes 31, and the screen 30 is adjacent to one end of the packing element 20 and has a tapered protrusion between the holes 31.
The conduit 40 is disposed at one side of the motor assembly 10 and the filling assembly 20, wherein the conduit 40 comprises a nozzle 41, and the nozzle 41 is disposed at one end of the conduit 40 and adjacent to one end of the filter screen 30 away from the filling assembly 20. In a preferred embodiment, a bottle of buffer solution is provided at the end of the conduit 40 remote from the nozzle 41.
The cutting assembly 50 is adjacent to the screen 30. The cutting assembly 50 has a reciprocating blade 51 and a first motor 52 connected to the blade 51, the blade 51 being adjacent to an end of the screen 30 remote from the packing assembly 20. In a preferred embodiment, the cutting assembly 50 is an ultrasonic blade set (ultrasonic instruments for tissue biopsy slicer) for tissue slicing. In another preferred embodiment, the cutting assembly 50 is a laser cutter assembly.
The diversion member 60 is disposed at an end of the hollow cylinder 21 of the filling assembly 20 opposite to the filter screen 30.
Referring to fig. 2 to 4, when the tissue crushing device of the present invention is used, a tissue is placed in the hollow cylinder 21, and the piston rod 22 is placed in the hollow cylinder 21; by one end of the piston rod 22 abutting against the motor assembly 10, once the motor assembly 10 is driven to push the piston rod 22, the tissue in the hollow cylinder 21 is squeezed and separated through the holes 31 of the filter screen 30; at the same time, the filter 30 is disposed adjacent to one end of the filling member 20 and is tapered between the holes 31 to facilitate tissue separation and extrusion into the holes 31. The exposed tissue passing through each aperture 31 is then severed by the cutting assembly 50 and simultaneously sprayed through the nozzle 41 with a buffer solution in the conduit 40 to obtain a finely divided tissue. In addition, the cut tissues are guided along the guiding member 60 sequentially by the guiding member 60 for collection.
Referring to fig. 5 and 6, the present invention further provides an apparatus having a tissue crushing device, which comprises the tissue crushing device and a frame 70.
The frame 70 includes a base 71, two plates 72, and a rotating plate 73. The plate bodies 72 are L-shaped, and one end of each plate body 72 is respectively connected with two sides of the base 71; the rotating plate 73 is rotatably disposed between the plates 72 and is away from one end of the base 71; the rotating plate 73 has a spring locking device 731 and a second motor 732 disposed on one side of the rotating plate 73, one end of the spring locking device 731 is fixed on one side of the rotating plate 73, and the other end is movably abutted against the hollow cylinder 21 of the filling assembly 20 of the tissue crushing device with elastic force; the conduit 40 and the cutting assembly 50 are disposed on a side of the rotating plate 73 away from the filling assembly 20.
When the apparatus with the tissue fine crushing device of the present invention is used, the end of the elastic locking device 731 abutting against the filling component 20 is movably pulled, so that the filling component 20 is detached from the tissue fine crushing device. When replaced with another tissue-containing filling assembly 20 and loaded into the tissue mincing device, the hollow cylinder 21 is facing upwards and the piston rod 22 is located below the hollow cylinder 21 to prevent tissue from flowing out. In preparation for the subsequent fine tissue separation, the rotating plate 73 is turned upside down so that the hollow cylinder 21 faces downward. The fine crushing action is as before, and when the fine crushing separation is completed, the rotating plate 73 is turned over again to make the hollow cylinder 21 upward. Because the rotating plate 73 is provided with the second motor 73 for assisting in overturning, and the tissue fine crushing device can automatically perform fine crushing and separation of tissues, manpower and time can be saved, and the fine crushing degree of the tissues, namely the volume of the tissues, can be unified.
The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. Any equivalent changes and modifications that can be made by one skilled in the art without departing from the spirit and principles of the invention should fall within the protection scope of the invention. It should be noted that the components of the present invention are not limited to the above-mentioned whole application, and various technical features described in the present specification can be selected to be used alone or in combination according to actual needs, so that the present invention naturally covers other combinations and specific applications related to the invention.
Claims (9)
1. An apparatus having tissue disruption means, said apparatus comprising:
tissue fine crushing device
A motor assembly;
a fill assembly, comprising:
a hollow cylinder; and the number of the first and second groups,
a piston rod movably arranged in the hollow cylinder in a penetrating way, wherein one end of the piston rod is propped against the motor component, so that the filling component is connected with the motor component;
the filter screen is arranged at one end of the hollow cylinder body of the filling assembly, which is far away from the motor assembly;
a conduit disposed on one side of the motor assembly and the filling assembly, wherein the conduit comprises:
a nozzle arranged at one end of the conduit and adjacent to one end of the filter screen far away from the filling assembly;
a cutting assembly adjacent to the screen; and the number of the first and second groups,
the flow guide piece is arranged at one end of the hollow cylinder of the filling assembly, which is opposite to the filter screen; and the number of the first and second groups,
a frame, comprising:
a base;
the two plate bodies are L-shaped, and one end of each plate body is respectively connected with the two sides of the base; and the number of the first and second groups,
the rotating plate can be rotatably arranged between the plate bodies and is far away from one end of the base; the rotating plate is provided with a second motor arranged on one side of the rotating plate; the tissue fine crushing device is arranged on the rotating plate.
2. The apparatus of claim 1, wherein the motor assembly is a stepper motor, a servo motor or an induction motor.
3. The apparatus of claim 1, wherein the screen has a plurality of holes, the screen being adjacent to an end of the filler assembly and having a conical protrusion between the holes.
4. The apparatus of any of claims 1 to 3, wherein the cutting assembly has a reciprocating blade and a first motor connected to the blade, and the blade is adjacent to an end of the filter screen remote from the filling assembly.
5. The apparatus of any of claims 1-3, wherein the cutting assembly is an ultrasonic blade set for tissue sectioning.
6. The apparatus of any of claims 1-3, wherein the cutting assembly is a laser knife assembly.
7. The apparatus of any of claims 1 to 3, wherein the rotating plate has a resilient locking means, one end of the resilient locking means is fixed to one side of the rotating plate, and the other end of the resilient locking means is capable of being movably and resiliently pressed against the hollow cylinder of the filling member of the tissue crushing means.
8. The apparatus of claim 7, wherein the conduit is disposed on a side of the rotating plate away from the filling assembly.
9. The apparatus of claim 7, wherein the cutting assembly is disposed on a side of the rotating plate away from the filling assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710080916.6A CN108424832B (en) | 2017-02-15 | 2017-02-15 | Device with tissue fine-crushing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710080916.6A CN108424832B (en) | 2017-02-15 | 2017-02-15 | Device with tissue fine-crushing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108424832A CN108424832A (en) | 2018-08-21 |
| CN108424832B true CN108424832B (en) | 2021-10-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710080916.6A Active CN108424832B (en) | 2017-02-15 | 2017-02-15 | Device with tissue fine-crushing device |
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| Country | Link |
|---|---|
| CN (1) | CN108424832B (en) |
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| CN201505937U (en) * | 2009-09-07 | 2010-06-16 | 邓少斌 | Food cutting machine |
| CN101796181A (en) * | 2007-05-04 | 2010-08-04 | 埃里克·松德斯特伦 | Slicing device |
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| CN204848894U (en) * | 2015-07-29 | 2015-12-09 | 国玺干细胞应用技术股份有限公司 | Stem cell preparation device |
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| CN205659755U (en) * | 2016-06-01 | 2016-10-26 | 新都区马家镇新越机械厂 | Meat supplied materials cuts up processingequipment |
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|---|---|---|---|---|
| US7101372B2 (en) * | 2001-04-06 | 2006-09-05 | Sherwood Sevices Ag | Vessel sealer and divider |
| US7146895B2 (en) * | 2004-10-21 | 2006-12-12 | Kong George Y | Sliding blade microtome |
| IT1400069B1 (en) * | 2010-05-20 | 2013-05-17 | Tremolada | DEVICE AND METHOD FOR THE PREPARATION OF FABRIC, IN PARTICULAR ADIPOUS FABRIC FOR TRANSPLANTATION OBTAINED FROM ADIPOUS LOBULAR MATERIAL EXTRACTED THROUGH LIPOSUCTION |
| CN204180837U (en) * | 2014-10-28 | 2015-03-04 | 吉林曙光农牧有限公司 | A kind of spiral is cooked meat machine |
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- 2017-02-15 CN CN201710080916.6A patent/CN108424832B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796181A (en) * | 2007-05-04 | 2010-08-04 | 埃里克·松德斯特伦 | Slicing device |
| CN201505937U (en) * | 2009-09-07 | 2010-06-16 | 邓少斌 | Food cutting machine |
| CN103997974A (en) * | 2011-11-08 | 2014-08-20 | 奥康细胞实验室公司 | Systems and methods for processing cells |
| CN104048861A (en) * | 2013-03-15 | 2014-09-17 | 徕卡生物系统努斯洛克有限公司 | Apparatus for working on histological samples |
| CN203458835U (en) * | 2013-08-19 | 2014-03-05 | 无锡加莱克色谱科技有限公司 | Homogenate pipe support for filling preparative liquid chromatogram column |
| CN204848894U (en) * | 2015-07-29 | 2015-12-09 | 国玺干细胞应用技术股份有限公司 | Stem cell preparation device |
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| CN205659755U (en) * | 2016-06-01 | 2016-10-26 | 新都区马家镇新越机械厂 | Meat supplied materials cuts up processingequipment |
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| Publication number | Publication date |
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| CN108424832A (en) | 2018-08-21 |
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Effective date of registration: 20221123 Address after: 6th Floor, No. 250, Section 1, Neihu Road, Neihu District, Taipei, Taiwan, China, China Patentee after: DRSIGNAL BIOTECHNOLOGY Co.,Ltd. Address before: Samoa City, Apia Patentee before: SCL BIOTECH Ltd. |