CN108420833A - A kind of probiotics with multiple coatings structure - Google Patents
A kind of probiotics with multiple coatings structure Download PDFInfo
- Publication number
- CN108420833A CN108420833A CN201710074767.2A CN201710074767A CN108420833A CN 108420833 A CN108420833 A CN 108420833A CN 201710074767 A CN201710074767 A CN 201710074767A CN 108420833 A CN108420833 A CN 108420833A
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- CN
- China
- Prior art keywords
- water
- hyaluronic acid
- added
- porous particles
- coating agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
Abstract
The present invention provides a kind of probiotics with multiple coatings structure, is made of following raw material：Protein, porous particles coating agent, hyaluronic acid and water-soluble polymer, the water-soluble polymer is primary coating, the hyaluronic acid is secondary coating, the porous particles coating agent is to be coated three times, the protein is four coatings, the water-soluble polymer is wrapped in inside hyaluronic acid, and the hyaluronic acid is wrapped in inside porous particles coating agent, and the porous particles coating agent is wrapped in protein interior；Compared with prior art, the present invention has following advantageous effect：Protein is protected for hydrochloric acid in gastric juice and bile acid; porous particles coating agent is for preventing moisture and wet air from entering; hyaluronic acid is for controlling moisture and improving adhesive force; for water-soluble polymer for preventing extraneous air from entering, this coating structure has good acid resistance, resistance to bile acidity, heat resistance.
Description
Technical field
The present invention is a kind of probiotics with multiple coatings structure, belongs to technical field of pharmaceutical biotechnology.
Background technology
Probiotics is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can generate
Definite health efficacy so as to improve host's microecological balance, play the active beneficial microorganism general name of beneficial effect.Human body moves
Beneficial bacterium or fungi mainly have in object：Clostridium butyricum, lactobacillus, Bifidobacterium, actinomyces, saccharomycete etc..Generation at present
The most powerful product of the function of being studied in boundary be mainly the above all kinds of microorganism groups at composite reactive probiotics, extensive use
In bioengineering, industrial or agricultural, food security and life and health field.
In the prior art, the probiotics that people eat, coating structure are single.
Invention content
In view of the deficienciess of the prior art, it is an object of the present invention to provide a kind of probiotics with multiple coatings structure,
To solve the problems mentioned in the above background technology.
To achieve the goals above, the present invention is to realize by the following technical solutions：One kind having multiple coatings knot
The probiotics of structure is made of following raw material：Protein, porous particles coating agent, hyaluronic acid and water-soluble polymer, institute
It is primary coating to state water-soluble polymer, and the hyaluronic acid is secondary coating, and the porous particles coating agent is to wrap three times
Clothing, the protein are four coatings, and the water-soluble polymer is wrapped in inside hyaluronic acid, and the hyaluronic acid is wrapped
It is rolled in inside porous particles coating agent, the porous particles coating agent is wrapped in protein interior.
Further, cockscomb fragment is added in acetone, soaked overnight to cockscomb dehydration is hardened, and is subsequently placed in porcelain dish
It is dried, is ground into powdery through pulverizer after drying, cockscomb powder is poured into enamel pot, 6~7 times of distilled water, stirring is added
Uniformly, it impregnating more than for 24 hours, so that cockscomb powder is fully swollen, then filter, collect filtrate, filter residue impregnates 3 times in the same way again,
Merge 3 filtered fluids, then filtrate is poured into ceramic cylinder, is added with stirring 10% solid sodium chloride, and isometric chlorine is added
It is imitative, after stirring 3h, water phase is separated, 95% ethyl alcohol of 2 times of volumes is added into the water phase separated, stirs evenly, stands 12h or more,
It is settled out hyaluronic acid, sediment is then filtered dry, obtains crude product after dry, crude product is re-dissolved in the 0.1mol/L sodium chloride of 4 times of volumes
In solution, pH=4.5~5 are adjusted with dilute hydrochloric acid, add isometric chloroform, after stratification, upper strata aqueous phase, water phase is sucked out
PH=7.5 are adjusted with 8% sodium hydroxide solution, pronase is added, is kept the temperature for 24 hours at 37 DEG C, then is extracted with isometric chloroform
Processing 2 times merges upper strata aqueous phase, and lower liquid recycles chloroform, and it is molten that 1% isometric cetylpyridinium chloride is added in water phase
Liquid stirs evenly, and staticly settles, filtering, collects precipitation, and precipitation is dissolved with the 0.4mol/L sodium chloride of 2~3 times of volumes again, mistake
It filters, adds 3 times of 95% ethyl alcohol of volume in filtrate, staticly settle, suck upper layer ethyl alcohol, lower layer is again with 95% ethyl alcohol repeated precipitation 2
Secondary, lower sediment thing hangs filter, dry, then is washed 2 times with acetone, is dried in vacuo, obtains the hyaluronic acid in raw material.
Further, the water-soluble polymer is a kind of organic semi-synthetic water-soluble high-molecular compound.
Beneficial effects of the present invention：A kind of probiotics with multiple coatings structure of the present invention, protein are used for hydrochloric acid in gastric juice
And bile acid protection, porous particles coating agent is for preventing moisture and wet air from entering, and hyaluronic acid is for controlling
Moisture and raising adhesive force, for preventing extraneous air from entering, this coating structure has good resistance to water-soluble polymer
Acid, resistance to bile acidity, heat resistance.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, with reference to
Specific implementation mode, the present invention is further explained.
The present invention provides a kind of technical solution：A kind of probiotics with multiple coatings structure, is made of following raw material：Egg
White matter, porous particles coating agent, hyaluronic acid and water-soluble polymer, water-soluble polymer are primary coating, hyalomitome
Acid is secondary coating, and porous particles coating agent is to be coated three times, and protein is four coatings, and water-soluble polymer is wrapped in
Inside hyaluronic acid, hyaluronic acid is wrapped in inside porous particles coating agent, and porous particles coating agent is wrapped in egg
Inside white matter.
Cockscomb fragment is added in acetone, soaked overnight to cockscomb dehydration is hardened, and is subsequently placed in porcelain dish and is dried,
It is ground into powdery through pulverizer after drying, cockscomb powder is poured into enamel pot, 6~7 times of distilled water are added, stir evenly, is impregnated
More than for 24 hours, so that cockscomb powder is fully swollen, then filter, collect filtrate, filter residue impregnates 3 times in the same way again, merges 3 mistakes
Filtrate, then filtrate is poured into ceramic cylinder, it is added with stirring 10% solid sodium chloride, and isometric chloroform is added, stirs 3h
Afterwards, water phase is separated, 95% ethyl alcohol of 2 times of volumes is added into the water phase separated, stirs evenly, 12h or more is stood, is settled out
Then bright matter acid is filtered dry sediment, crude product is obtained after dry, crude product is re-dissolved in the 0.1mol/L sodium chloride solutions of 4 times of volumes, is used
Dilute hydrochloric acid adjusts pH=4.5~5, adds isometric chloroform, and after stratification, upper strata aqueous phase, 8% hydroxide of water phase is sucked out
Sodium solution adjusts pH=7.5, and pronase is added, and keeps the temperature for 24 hours at 37 DEG C, then with isometric chloroform extraction processing 2 times, conjunction
And upper strata aqueous phase, lower liquid recycle chloroform, and 1% isometric cetylpyridinium chloride solution is added in water phase, stirring is equal
It is even, it staticly settles, filters, collect precipitation, precipitation is dissolved with the 0.4mol/L sodium chloride of 2~3 times of volumes again, filtering, in filtrate
3 times of 95% ethyl alcohol of volume are added, are staticly settled, suck upper layer ethyl alcohol, again with 95% ethyl alcohol repeated precipitation 2 times, lower layer is heavy for lower layer
Starch hangs filter, dry, then is washed 2 times with acetone, is dried in vacuo, obtains the hyaluronic acid in raw material.
Water-soluble polymer is a kind of organic semi-synthetic water-soluble high-molecular compound.
As an embodiment of the present invention：A kind of probiotics with multiple coatings structure of the present invention, protein are used
It is protected in hydrochloric acid in gastric juice and bile acid, porous particles coating agent for preventing moisture and wet air from entering, use by hyaluronic acid
In control moisture and adhesive force is improved, for preventing extraneous air from entering, this coating structure has good water-soluble polymer
Acid resistance well, resistance to bile acidity, heat resistance.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill
For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from the present invention spirit or
In the case of essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action
Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state
Bright restriction, it is intended that including all changes that come within the meaning and range of equivalency of the claims in the present invention
It is interior.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.
Claims (3)
1. a kind of probiotics with multiple coatings structure, it is characterised in that：It is made of following raw material：Protein, porosity grain
Sub- coating agent, hyaluronic acid and water-soluble polymer, the water-soluble polymer are primary coating, and the hyaluronic acid is two
Secondary coating, the porous particles coating agent are to be coated three times, and the protein is four coatings, the water-soluble polymer quilt
It is wrapped in inside hyaluronic acid, the hyaluronic acid is wrapped in inside porous particles coating agent, and the porous particles apply
Layer agent is wrapped in protein interior.
2. a kind of probiotics with multiple coatings structure according to claim 1, it is characterised in that：Cockscomb fragment is added
Enter into acetone, soaked overnight to cockscomb dehydration is hardened, and is subsequently placed in porcelain dish and is dried, is ground into through pulverizer after dry
Powdery pours into cockscomb powder in enamel pot, and 6~7 times of distilled water are added, stir evenly, and impregnates more than for 24 hours, keeps cockscomb powder abundant
Swelling, is then filtered, and collects filtrate, and filter residue impregnates 3 times in the same way again, merges 3 filtered fluids, then filtrate is poured into ceramics
In cylinder, it is added with stirring 10% solid sodium chloride, and isometric chloroform is added, after stirring 3h, water phase is separated, to what is separated
95% ethyl alcohol of 2 times of volumes is added in water phase, stirs evenly, stands 12h or more, is settled out hyaluronic acid, is then filtered dry precipitation
Object, it is dry after crude product, crude product are re-dissolved in the 0.1mol/L sodium chloride solutions of 4 times of volumes, with dilute hydrochloric acid adjust pH=4.5~
5, it adds isometric chloroform, after stratification, is sucked out upper strata aqueous phase, water phase 8% sodium hydroxide solution adjusting pH=7.5,
Pronase is added, keeps the temperature for 24 hours at 37 DEG C, then with isometric chloroform extraction processing 2 times, merging upper strata aqueous phase, subnatant
Body recycles chloroform, and 1% isometric cetylpyridinium chloride solution is added in water phase, stirs evenly, staticly settles, and filters, and receives
Collection precipitation, precipitation are dissolved with the 0.4mol/L sodium chloride of 2~3 times of volumes again, filtering, and 3 times of 95% second of volume are added in filtrate
Alcohol staticly settles, and sucks upper layer ethyl alcohol, and again with 95% ethyl alcohol repeated precipitation 2 times, lower sediment thing hangs filter, dry, then uses for lower layer
Acetone washs 2 times, and vacuum drying obtains the hyaluronic acid in raw material.
3. a kind of probiotics with multiple coatings structure according to claim 1, it is characterised in that：The water-soluble poly
It is a kind of organic semi-synthetic water-soluble high-molecular compound to close object.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710074767.2A CN108420833A (en) | 2017-02-12 | 2017-02-12 | A kind of probiotics with multiple coatings structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710074767.2A CN108420833A (en) | 2017-02-12 | 2017-02-12 | A kind of probiotics with multiple coatings structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108420833A true CN108420833A (en) | 2018-08-21 |
Family
ID=63147222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710074767.2A Pending CN108420833A (en) | 2017-02-12 | 2017-02-12 | A kind of probiotics with multiple coatings structure |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816627A (en) * | 2010-04-16 | 2010-09-01 | 浙江大学 | Synergistic treatment type multi-material sustained-release eye drop and preparation method |
| CN103140243A (en) * | 2010-08-09 | 2013-06-05 | 迪格玛斯玛特有限公司 | Probiotic liquid food products |
| CN103347395A (en) * | 2010-12-06 | 2013-10-09 | 德嘉玛贝里尔有限公司 | Composition and method for improving stability and extending shelf life of probiotic bacteria and food products thereof |
| CN104431354A (en) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | Probiotic microcapsules as well as preparation method and application thereof |
| CN104435283A (en) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | Probiotic microcapsule added with prebiotics and preparation method thereof |
| CN106377494A (en) * | 2016-12-12 | 2017-02-08 | 珍尔姿（广州）化妆品有限公司 | Whitening and wrinkle removal functional mask |
-
2017
- 2017-02-12 CN CN201710074767.2A patent/CN108420833A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816627A (en) * | 2010-04-16 | 2010-09-01 | 浙江大学 | Synergistic treatment type multi-material sustained-release eye drop and preparation method |
| CN103140243A (en) * | 2010-08-09 | 2013-06-05 | 迪格玛斯玛特有限公司 | Probiotic liquid food products |
| CN103347395A (en) * | 2010-12-06 | 2013-10-09 | 德嘉玛贝里尔有限公司 | Composition and method for improving stability and extending shelf life of probiotic bacteria and food products thereof |
| CN104431354A (en) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | Probiotic microcapsules as well as preparation method and application thereof |
| CN104435283A (en) * | 2014-11-17 | 2015-03-25 | 金陵科技学院 | Probiotic microcapsule added with prebiotics and preparation method thereof |
| CN106377494A (en) * | 2016-12-12 | 2017-02-08 | 珍尔姿（广州）化妆品有限公司 | Whitening and wrinkle removal functional mask |
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Application publication date: 20180821 |
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