CN109232920A - Isinglass/sodium alginate dual network composite hydrogel, preparation method and gained probiotic microcapsule - Google Patents
Isinglass/sodium alginate dual network composite hydrogel, preparation method and gained probiotic microcapsule Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/04—Alginic acid; Derivatives thereof
Abstract
The present invention proposes a kind of isinglass/sodium alginate dual network composite hydrogel, preparation method and gained probiotic microcapsule; belong to microcapsules field; when being able to solve isinglass gel and sodium alginate gel and being applied as single network gel; have the shortcomings that mechanical property and stability are poor; it is unfavorable for the embedding of probiotics; cause probiotics embedding rate low, the problem small to the protection of probiotics.The technical solution includes preparing sodium alginate soln, by its abundant aquation；Isinglass is added in the sodium alginate soln good to above-mentioned aquation, heating induction processing then is carried out to mixed solution, it is cooling, obtain gel mixed liquor；Above-mentioned gel mixed liquor is at the uniform velocity added in the first ionic calcium soln and is formed, then gel after molding is added in the second ionic calcium soln and hardens.The present invention can be applied to probiotics embedding aspect, have embedding rate height, the high feature of probiotics survival rate.
Description
Technical field
The invention belongs to microcapsules field more particularly to a kind of isinglass/sodium alginate dual network composite hydrogels, its system
Preparation Method and gained probiotic microcapsule.
Background technique
A kind of medium of the probiotics as health transmitting, has many Beneficial Effects to human health, such as can be people
Body provides lactic acid, furthermore the substance decomposition that indigestibility can be decomposed can also play protective action to infecting for harmful pathogen.
But vigor of the probiotics in food be easy be heat-treated, be machined, the physical state of food matrix, chemical micro-environment,
The influence of the factors such as storage conditions, in addition, after the intake human body of the food containing probiotic ingredient, the effect meeting of gastric acid and bile acid
Destroy most probiotics.Therefore, maintaining activity of probiotic is the key that its development and application.
Currently, microcapsules technology is to protect probiotics the most practical and one of effective means.Effect microcapsule wall is one layer
Probiotics, is embedded among cyst wall by fine and close and semipermeable colloidal film by method physically or chemically so that probiotics with
External environment separates, and destruction of the external environment to probiotics is reduced, to improve its survival rate.Hydrogel had both had centainly
Fluid properties have good viscoplasticity and biocompatibility again, and macromolecular chain therein is cross-linked with each other to be formed under given conditions
Three-dimensional net structure is swollen in solvent and is not dissolved, and can be used as the occlusion vehicle of drug, nutriment, histocyte etc., because
And it is commonly used for microcapsule systems.Wherein, isinglass gel and sodium alginate gel be due to excellent performance, usually by with
In microcapsule systems.
However, isinglass gel and sodium alginate gel are as single network gel by application, there are mechanical property and surely
The disadvantage of qualitative difference, is unfavorable for the embedding of probiotics, causes probiotics embedding rate low, small to the protection of probiotics.
Summary of the invention
The present invention is existing when being applied for above-mentioned isinglass gel and sodium alginate gel as single network gel
Technical problem, propose a kind of gel strength it is big, to probiotics embedding rate the high, isinglass/seaweed big to probiotics protection
Sour sodium dual network composite hydrogel, preparation method and gained probiotic microcapsule.
In order to achieve the above object, the technical solution adopted by the present invention are as follows:
Isinglass/sodium alginate dual network composite hydrogel preparation method, comprising the following steps:
Sodium alginate soln is prepared, by its abundant aquation；
Isinglass is added in the sodium alginate soln good to above-mentioned aquation, then mixed solution is carried out at heating induction
Reason, it is cooling, obtain gel mixed liquor；
Above-mentioned gel mixed liquor is at the uniform velocity added in the first ionic calcium soln and is formed, gel after molding is then added the
It is hardened in two ionic calcium solns.Preferably, the treatment temperature for carrying out heating induction processing to mixed solution is 60-80
℃。
Preferably, the concentration of sodium alginate is 0.015g/mL in the gel mixed liquor.
Preferably, the concentration of isinglass is 0.01-0.06g/mL in the gel mixed liquor.
Preferably, first ionic calcium soln and the second ionic calcium soln include calcium chloride solution or calcium sulphate soln
In any one.
Preferably, the concentration of first ionic calcium soln is 0.1-0.15mol/L, second ionic calcium soln
Concentration is 0.3-0.35mol/L.
Technical solution of the present invention additionally provides the isinglass/seaweed being prepared such as any of the above-described technical solution the method
Sour sodium dual network composite hydrogel.
Technical solution of the present invention additionally provides isinglass/sodium alginate dual network compound water congealing as described in above-mentioned technical proposal
The probiotic microcapsule that glue is prepared.
Preferably, the probiotics is embedded in the capsule being made of isinglass/sodium alginate dual network composite hydrogel
In wall.
Preferably, the probiotics is embedded in the capsule being made of isinglass/sodium alginate dual network composite hydrogel
In wall, preparation method the following steps are included:
Sodium alginate soln is prepared, by its abundant aquation；
Isinglass is added in the sodium alginate soln good to above-mentioned aquation, then mixed solution is carried out at heating induction
Reason, it is cooling, obtain gel mixed liquor；
Gel is added in concentrated probiotics liquid by the ratio for being 1:20 in the volume ratio of concentrated probiotics liquid and gel mixed liquor
In mixed liquor, stir evenly；
The gel mixed liquor dissolved with concentrated probiotics liquid is at the uniform velocity added in the first ionic calcium soln and is formed, then will
Gel after molding is added in the second ionic calcium soln and hardens.
Compared with prior art, the advantages and positive effects of the present invention are:
1, isinglass/sodium alginate dual network composite hydrogel has the characteristics that gel strength is big, moisture content is high；
2, the probiotic microcapsule being prepared using isinglass/sodium alginate dual network composite hydrogel as cyst wall, tool
There are embedding rate height, the high feature of probiotics survival rate.
Detailed description of the invention
Fig. 1 is gel SEM result schematic diagram provided by the embodiment of the present invention, wherein (a) is what comparative example 1 was prepared
Sodium alginate list network gel, (b) composite hydrogel being prepared for embodiment 2, (c) is answered for what embodiment 4 was prepared
Heshui gel, (d) composite hydrogel being prepared for embodiment 6；
Fig. 2 is gel moisture content and specific retention result schematic diagram provided by the embodiment of the present invention, wherein No. 0 sample is
The sodium alginate list network gel that comparative example 1 is prepared, the composite hydrogel that No. 1 sample is prepared for embodiment 1, No. 2
Sample is the composite hydrogel that embodiment 2 is prepared, and No. 3 samples are the composite hydrogel that embodiment 3 is prepared, No. 4 samples
Product are the composite hydrogel that embodiment 4 is prepared, and No. 5 samples are the composite hydrogel that embodiment 5 is prepared, No. 6 samples
The composite hydrogel being prepared for embodiment 6；
Fig. 3 is that isinglass provided by the embodiment of the present invention/sodium alginate dual network composite hydrogel embeds probiotics
Flow diagram；
Fig. 4 is Bifidobacterium embedding rate result schematic diagram provided by the embodiment of the present invention, wherein No. 0 sample is comparative example
2 probiotic microcapsules being prepared, No. 1 sample are the probiotic microcapsule that embodiment 7 is prepared, and No. 2 samples are to implement
The probiotic microcapsule that example 8 is prepared, No. 3 samples are the probiotic microcapsule that embodiment 9 is prepared, and No. 4 samples are real
The probiotic microcapsule that example 10 is prepared is applied, No. 5 samples are the probiotic microcapsule that embodiment 11 is prepared, No. 6 samples
The probiotic microcapsule being prepared for embodiment 12；
Fig. 5 is probiotics survival experiment result schematic diagram provided by the embodiment of the present invention；
Wherein, the lowercase difference in figure represents significant difference.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The embodiment of the invention provides isinglass/sodium alginate dual network composite hydrogel preparation method, including it is following
Step:
S1: sodium alginate soln is prepared, by its abundant aquation.In this step, sodium alginate soln is prepared, by alginic acid
Sodium solution is placed under 4 DEG C of environment and refrigerates for 24 hours, dissolves it sufficiently, aquation.
S2: being added isinglass in the sodium alginate soln good to above-mentioned aquation, then carries out heating induction to mixed solution
Processing, it is cooling, obtain gel mixed liquor.In this step, isinglass has low temperature from gel and thermal reversibility, heated denaturation
For isinglass afterwards when temperature is reduced to gel critical-temperature or less, isinglass molecule is from random coil to triple helix structure
Transformation, to form the three-dimensional net structure of gel.
S3: above-mentioned gel mixed liquor is at the uniform velocity added in the first ionic calcium soln and is formed, then adds gel after molding
Enter and is hardened in the second ionic calcium soln.In this step, in the presence of calcium ion, the adhesion strength between alginate is conducive to molecule
Between bridge joint so that surface is initially formed the gel layer of an interconnection.And calcium ion can preferentially with alginate middle ancient times sieve
The carboxyls of uronic acid units combines, and alginate molecule and isinglass/the sodium alginate gel network for closing on calcium ion it
Between form bridge, to form final network structure.
In a preferred embodiment, the treatment temperature for carrying out heating induction processing to mixed solution is 60-80 DEG C.?
In the embodiment, carrying out heat treatment to isinglass may be implemented isinglass protein uncoiling degenerative process.It is understood that
The treatment temperature can also be any point value in 65 DEG C, 70 DEG C, 75 DEG C and its range.
In a preferred embodiment, the concentration of sodium alginate is 0.015g/mL in the gel mixed liquor.In the embodiment
In, the concentration of sodium alginate in gel mixed liquor is specifically defined, the reason is that, when sodium alginate concentration is higher than 0.015g/mL
When, the presentation of gel color burn is yellowish, and transparency and brightness are substantially reduced, and hardness increases.
In a preferred embodiment, the concentration of isinglass is 0.01-0.06g/mL in the gel mixed liquor.In the implementation
In example, the concentration of isinglass in gel mixed liquor is specifically defined, the reason is that, compound in isinglass/sodium alginate dual network
In hydrogel, using sodium alginate as basic framework, isinglass can keep more complete network as a kind of protein molecule
Structure, and with the increase of isinglass concentration, plural gel internal structure more dense regular, however, working as the concentration of isinglass
When higher than 0.06g/mL, solution viscosity increases, and squeezes out difficulty, obvious trailing phenomenon or even skin breakage occurs, causes compound solidifying
There is obvious slight crack in glue surface, and plural gel color shows light light yellow.It is understood that the concentration of the isinglass
It can also be any point value in 0.02g/mL, 0.03g/mL, 0.04g/mL, 0.05g/mL and its range.
In a preferred embodiment, first ionic calcium soln and the second ionic calcium soln include calcium chloride solution or sulphur
Any one in sour calcium solution.It is understood that for the first ionic calcium soln cited by the present embodiment and the second calcium
Solion can also be that those skilled in the art pass through the other materials for reasonably selecting and adjusting in this field.
In a preferred embodiment, the concentration of first ionic calcium soln be 0.1-0.15mol/L, second calcium from
The concentration of sub- solution is 0.3-0.35mol/L.In this embodiment, it specifically defines molten for the molding calcium ion of plural gel
The concentration of liquid concentration and the ionic calcium soln for plural gel hardening, wherein sodium alginate, which has to contact with calcium ion, just to be stood
Carve molding characteristic, it is therefore, lower for the molding first ionic calcium soln concentration of plural gel, otherwise, the calcium of high concentration from
Sub- solution will cause that gel composite network structure is uneven, and the blocky phenomenon in part irregularly even occurs in gel shape；Second
Ionic calcium soln is used for gel hardening processing, selects the calcium ion concentration of higher concentration, it is therefore intended that enters more calcium ions
And be dispersed in inside gel, further crosslinking is completed, to obtain the network structure of more dense regular.It is understood that
, the first ionic calcium soln concentration can also be 0.11mol/L, 0.12 mol/L, 0.13mol/L, 0.14mol/L and
Any point value within the scope of its, the second ionic calcium soln concentration can also be 0.31mol/L, 0.32mol/L, 0.33mol/
L, any point value in 0.34mol/L and its range.
The embodiment of the invention also provides isinglass/alginic acids that such as above-mentioned any embodiment the method is prepared
Sodium dual network composite hydrogel.In this embodiment, isinglass/sodium alginate dual network composite hydrogel has the double nets of interpenetrating type
Network structure has gel strength big, the high feature of specific retention.
The embodiment of the invention also provides isinglass/sodium alginate dual network composite hydrogel systems as described in above-described embodiment
Standby obtained probiotic microcapsule.In this embodiment, it is prepared by isinglass/sodium alginate dual network composite hydrogel
Probiotic microcapsule has embedding rate height, the high feature of probiotics survival rate.
In a preferred embodiment, the probiotics is embedded in by isinglass/sodium alginate dual network composite hydrogel
In the cyst wall of composition.In this embodiment, using isinglass/sodium alginate dual network composite hydrogel as probiotic microcapsule
Cyst wall, wherein using sodium alginate as basic framework, it is ensured that the microcapsules carry out protopepsia in gastric environment, and fish is bright
Glue can keep more complete network structure as a kind of protein to have good protective effect, micro- glue to probiotics
Capsule, which reaches in intestines environment, achievees the purpose that sustained release by further digesting and assimilating.
In a preferred embodiment, the probiotics is embedded in by isinglass/sodium alginate dual network composite hydrogel
In the cyst wall of composition, preparation method the following steps are included:
S1: sodium alginate soln is prepared, by its abundant aquation；
S2: being added isinglass in the sodium alginate soln good to above-mentioned aquation, then carries out heating induction to mixed solution
Processing, it is cooling, obtain gel mixed liquor；
S3: concentrated probiotics liquid is added the ratio for being 1:20 in the volume ratio of concentrated probiotics liquid and gel mixed liquor
In gel mixed liquor, stir evenly；
S4: the gel mixed liquor dissolved with concentrated probiotics liquid is at the uniform velocity added in the first ionic calcium soln and is formed, so
Gel after molding is added in the second ionic calcium soln afterwards and is hardened.In this embodiment, concentrated probiotics liquid be probiotics with
The mixed liquor of physiological saline, wherein the volume ratio of probiotics and physiological saline is 1:10.The present embodiment specifically defines concentration benefit
The volume ratio of raw bacterium solution and gel mixed liquor improves probiotics it is advantageous that probiotics, which may be implemented, preferably embeds effect
Embedding rate, guarantee the survival rate of probiotics.
Isinglass provided by the embodiment of the present invention/sodium alginate dual network compound water congealing is introduced in detail in order to become apparent from
Glue, preparation method and gained probiotic microcapsule, are described below in conjunction with specific embodiment.
Embodiment 1
A certain amount of sodium alginate powder is weighed, it is molten to be configured to the sodium alginate that mass concentration is 0.015g/mL with distilled water
Liquid, magnetic agitation 1h dissolve sodium alginate sufficiently, are placed in 4 DEG C of refrigerator cold-storages for 24 hours, make its abundant aquation.It weighs a certain amount of
Isinglass powder be added in the good sodium alginate soln of aquation, be heated to 60 DEG C using magnetic stirring apparatus, after stirring 30min,
It is cooling, obtain gel mixed liquor, wherein the concentration of isinglass is 0.01g/mL.It will with 1mL syringe (needle mouth diameter 0.45mm)
Gel mixed liquor is at the uniform velocity instilled in 0.15mol/L calcium chloride solution and is formed, and molding gel beads are then placed in 0.35 mol/L
1h is hardened in calcium chloride solution, obtains isinglass/sodium alginate dual network composite hydrogel.
Embodiment 2
Isinglass/the preparation method is the same as that of Example 1 for sodium alginate dual network composite hydrogel, the difference is that, isinglass
Concentration be 0.02g/mL.
Embodiment 3
Isinglass/the preparation method is the same as that of Example 1 for sodium alginate dual network composite hydrogel, the difference is that, isinglass
Concentration be 0.03g/mL.
Embodiment 4
Isinglass/the preparation method is the same as that of Example 1 for sodium alginate dual network composite hydrogel, the difference is that, isinglass
Concentration be 0.04g/mL.
Embodiment 5
Isinglass/the preparation method is the same as that of Example 1 for sodium alginate dual network composite hydrogel, the difference is that, isinglass
Concentration be 0.05g/mL.
Embodiment 6
Isinglass/the preparation method is the same as that of Example 1 for sodium alginate dual network composite hydrogel, the difference is that, isinglass
Concentration be 0.06g/mL.
Embodiment 7
The freeze-dried vaccine powder of Bifidobacterium is inoculated in MRS broth bouillon, goes to solid medium culture after activation 3 times
For 24 hours, the single colony inoculation of picking cultivates 48h under 37 DEG C of constant temperatures into the MRS broth bouillon of 5mL.The bacterium that will be prepared
Liquid is placed in centrifuge tube, and 5000r is centrifuged 12min, discards supernatant liquid, 2mL physiological saline is added, concentration bacterium solution is made.
A certain amount of sodium alginate powder is weighed, it is molten to be configured to the sodium alginate that mass concentration is 0.015g/mL with distilled water
Liquid, magnetic agitation 1h dissolve sodium alginate sufficiently, are placed in 4 DEG C of refrigerator cold-storages for 24 hours, make its abundant aquation.It weighs a certain amount of
Isinglass powder be added in the good sodium alginate soln of aquation, be heated to 60 DEG C using magnetic stirring apparatus, after stirring 30min,
It is cooling, obtain gel mixed liquor, wherein the concentration of isinglass is 0.01g/mL.Ultrasonic degassing (40 is carried out to gel mixed liquor
DEG C, 10min), 45 DEG C of heat preservations.It will be dense according to the ratio that concentration bacterium solution and gel mixed liquor are 1:20 (v/v) after temperature is constant
Contracting bacterium solution is added in gel mixed liquor, is slowly stirred 20min, with 1mL syringe (needle mouth diameter 0.45mm) by gel mixed liquor
It at the uniform velocity instills in 0.15mol/L calcium chloride solution and forms, then molding gel beads are placed in 0.35mol/L calcium chloride solution
1h is hardened, obtains probiotic microcapsule, embedding process is shown in Fig. 3.
Embodiment 8
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0.02g/mL.
Embodiment 9
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0.03g/mL.
Embodiment 10
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0.04g/mL.
Embodiment 11
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0.05g/mL.
Embodiment 12
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0.06g/mL.
Comparative example 1
The preparation method is the same as that of Example 1 for hydrogel, the difference is that, the concentration of isinglass is 0g/mL.
Comparative example 2
The preparation method of probiotic microcapsule with embodiment 7, the difference is that, the concentration of isinglass is 0 g/mL.
Performance test
1, scanning electron microscope (SEM) microstructure observing
After freeze-drying sample is pasted in sample disc with two-sided conductive carbon trademark adhesive, d.c. sputtering vacuum metal spraying is then usedAcceleration voltage is 3kV, is scattered and is scanned using SEI secondary electron imaging system reverse, observes sample surfaces shape
State, surface topography are shown in Fig. 1.
2, gel moisture content and specific retention measurement
Gel measurement of water-content coefficient:
It takes certain mass gel beads in aluminium box with cover, is placed in 105 DEG C of baking ovens and heats 5-6h to its constant weight, calculate aqueous
Rate.
The measurement of gel specific retention:
Take a certain amount of gel beads to be put into the ultra-filtration centrifuge tube inner tube weighed, be placed in a centrifuge centrifugation (5000 r/min,
After 20min).Inner tube is taken out, surface moisture is dried, claims its quality, calculates specific retention, gel moisture content and specific retention measurement result
See Fig. 2.
3, gel embedding Bifidobacterium embedding rate measures
Take 1mL microcapsules in 9mL 0.1g/mL sodium citrate solution, 10 min are heated in 45 DEG C of waters bath with thermostatic control, and be vortexed shake
It swings and opens one's purse, count plate.
The wet capsule viable count=G1 × V1 of 1mL after opening one's purse
In formula:
G1-1mL solves the viable count (CFU/mL) in cyst fluid；
V1-solution cyst fluid volume (mL)；
Gel embedding Bifidobacterium embedding rate measurement result is shown in Fig. 4.
4, gel embedding Bifidobacterium external digestion model foundation
With simulated gastric fluid (SGF): taking 0.1mol/L dilute hydrochloric acid 16.4mL, add water about 80mL and pepsin 1g, after stirring evenly
Water is added to be settled to 100mL.
With simulated intestinal fluid (SIF): potassium dihydrogen phosphate 0.68g being taken to add water 50mL.With the sodium hydroxide solution of 0.4% (w/v)
Adjust pH to 6.8；It separately takes pancreatin 1g that appropriate amount of water is added to make it dissolve, after two solution are mixed, water is added to be settled to 100mL.
1mL sample is respectively taken to be placed in 10mL simulated gastric fluid and simulated intestinal fluid, shaking table shakes (37 DEG C, 100rpm, 20min).
Postdigestive microcapsules are filtered, 9mL 0.1g/mL sodium citrate solution is added, (45 DEG C, 10min) are heated in water bath with thermostatic control, are vortexed
Concussion is opened one's purse, count plate.Using the free bacterium solution of 1mL as control.
Probiotics survival experiment result is shown in Fig. 5.
By Fig. 1 experimental result it is found that sodium alginate single gel structure is sparse, irregular structure after freeze-drying.And it is compound solidifying
It is still able to maintain more complete chondritic after jelly is dry, and with the increase of isinglass concentration, internal structure more dense regular.
By Fig. 2 experimental result it can be found that with isinglass concentration increase (in comparative example 1 isinglass concentration be 0), it is multiple
Closing gel moisture content has decline trend but changes not significant.Specific retention significantly rises and is 0.05g/mL in isinglass mass fraction
When reach peak value, thus it is speculated that reason may be the addition of isinglass so that plural gel network is finer and close, forms capillary siphon and makees
With to keep more moisture, and the reticular structure of this densification delivers advantageously the embedding of subsequent nutriment.
Fig. 4 Bifidobacterium embedding rate is the results showed that when sodium alginate concentration is 0.015g/mL, and embedding rate is with fish
Downward trend after first rising is presented in the raising of gelatin mass concentration, wherein No. 5 samples, that is, isinglass mass concentration is 0.05g/
When mL, embedding rate obtains maximum value 87.25%, and embedding effect is best.In addition, the microcapsules pair being prepared by embodiment 7-12
The embedding effect of probiotics is superior to the microcapsules being prepared by comparative example 2 to the embedding effect of probiotics.Analysis result pushes away
Survey may be the increase due to isinglass concentration, generate fine and close network structure with sodium alginate cross-linking, thus can more effectively by
Probiotics is trapped in inside wall material.
By Fig. 5 probiotics survival experiment result it can be found that plural gel has good protective effect to probiotics.Its
In, when isinglass concentration is 0.05g/ml, survival rate of the Bifidobacterium in gastric juice, intestinal juice is respectively 31.38% and
61.71%, it is best to the protecting effect of probiotics much higher than the survival rate 0.06% and 30.47% of free bacterium.
Claims (10)
1. isinglass/sodium alginate dual network composite hydrogel preparation method, which comprises the following steps:
Sodium alginate soln is prepared, by its abundant aquation；
Isinglass is added in the sodium alginate soln good to above-mentioned aquation, heating induction processing then is carried out to mixed solution, it is cold
But, gel mixed liquor is obtained；
Above-mentioned gel mixed liquor is at the uniform velocity added in the first ionic calcium soln and is formed, the second calcium then is added in gel after molding
It is hardened in solion.
2. the method according to claim 1, wherein the processing for carrying out heating induction processing to mixed solution
Temperature is 60-80 DEG C.
3. the method according to claim 1, wherein the concentration of sodium alginate is in the gel mixed liquor
0.015g/mL。
4. the method according to claim 1, wherein the concentration of isinglass is 0.01- in the gel mixed liquor
0.06g/mL。
5. the method according to claim 1, wherein first ionic calcium soln and the second ionic calcium soln packet
Include any one in calcium chloride solution or calcium sulphate soln.
6. the method according to claim 1, wherein the concentration of first ionic calcium soln is 0.1-
0.15mol/L, the concentration of second ionic calcium soln are 0.3-0.35mol/L.
7. isinglass/sodium alginate dual network the composite hydrogel being prepared such as any one of claim 1-6 the method.
8. the probiotic microcapsule that isinglass as claimed in claim 7/sodium alginate dual network composite hydrogel is prepared.
9. probiotic microcapsule according to claim 8, which is characterized in that the probiotics be embedded in by isinglass/
In the cyst wall that sodium alginate dual network composite hydrogel is constituted.
10. probiotic microcapsule according to claim 9, which is characterized in that the probiotics be embedded in by isinglass/
Sodium alginate dual network composite hydrogel constitute cyst wall in, preparation method the following steps are included:
Sodium alginate soln is prepared, by its abundant aquation；
Isinglass is added in the sodium alginate soln good to above-mentioned aquation, heating induction processing then is carried out to mixed solution, it is cold
But, gel mixed liquor is obtained；
Concentrated probiotics liquid is added gel and mixed by the ratio for being 1:20 in the volume ratio of concentrated probiotics liquid and gel mixed liquor
In liquid, stir evenly；
The gel mixed liquor dissolved with concentrated probiotics liquid is at the uniform velocity added in the first ionic calcium soln and is formed, it then will molding
Gel afterwards is added in the second ionic calcium soln and hardens.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256856A (en) * | 2019-07-01 | 2019-09-20 | 重庆科技学院 | Sodium alginate is porous congeals glue and preparation method thereof for a kind of injectable gelatin-with shape memory |
| CN111375361A (en) * | 2020-03-17 | 2020-07-07 | 北京唐颐惠康生物医学技术有限公司 | Nano trehalose 3D microcapsule for large-scale stem cell culture |
| CN111588022A (en) * | 2020-05-27 | 2020-08-28 | 天津裕邦食品配料有限公司 | Food additive and preparation method thereof |
| CN113717404A (en) * | 2021-08-25 | 2021-11-30 | 北京航空航天大学 | In-situ forming method of liquid metal gel electrons |
| CN114672480A (en) * | 2022-04-29 | 2022-06-28 | 浙江工商大学 | Lactobacillus plantarum gel bead and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006024491A1 (en) * | 2004-08-30 | 2006-03-09 | Interstitial Therapeutics | Methods and compositions for the treatment of cell proliferation |
| CN101856604A (en) * | 2010-06-04 | 2010-10-13 | 上海理工大学 | Method for preparing probiotic microcapsules by using electrostatic spraying |
-
2018
- 2018-09-11 CN CN201811055528.3A patent/CN109232920A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006024491A1 (en) * | 2004-08-30 | 2006-03-09 | Interstitial Therapeutics | Methods and compositions for the treatment of cell proliferation |
| CN101856604A (en) * | 2010-06-04 | 2010-10-13 | 上海理工大学 | Method for preparing probiotic microcapsules by using electrostatic spraying |
Non-Patent Citations (1)
| Title |
|---|
| 陈海华: "明胶的凝胶特性及多糖对明胶凝胶特性的影响", 《粮油加工》 * |
Cited By (6)
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|---|---|---|---|---|
| CN110256856A (en) * | 2019-07-01 | 2019-09-20 | 重庆科技学院 | Sodium alginate is porous congeals glue and preparation method thereof for a kind of injectable gelatin-with shape memory |
| CN111375361A (en) * | 2020-03-17 | 2020-07-07 | 北京唐颐惠康生物医学技术有限公司 | Nano trehalose 3D microcapsule for large-scale stem cell culture |
| CN111588022A (en) * | 2020-05-27 | 2020-08-28 | 天津裕邦食品配料有限公司 | Food additive and preparation method thereof |
| CN113717404A (en) * | 2021-08-25 | 2021-11-30 | 北京航空航天大学 | In-situ forming method of liquid metal gel electrons |
| CN113717404B (en) * | 2021-08-25 | 2023-07-14 | 北京航空航天大学 | In-situ forming method of liquid metal gel electrons |
| CN114672480A (en) * | 2022-04-29 | 2022-06-28 | 浙江工商大学 | Lactobacillus plantarum gel bead and preparation method thereof |
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