CN108420803A - Nano enzyme adhesive bandage, preparation method and application method - Google Patents
Nano enzyme adhesive bandage, preparation method and application method Download PDFInfo
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- CN108420803A CN108420803A CN201810399824.9A CN201810399824A CN108420803A CN 108420803 A CN108420803 A CN 108420803A CN 201810399824 A CN201810399824 A CN 201810399824A CN 108420803 A CN108420803 A CN 108420803A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K33/44—Elemental carbon, e.g. charcoal, carbon black
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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Abstract
A kind of nano enzyme adhesive bandage of present invention offer, preparation method and application method, belong to adhesive bandage technical field.Solve the problem of that drug that existing adhesive bandage uses cannot promote the cell of the breeding of wound cells and wound circumference to have very strong toxic effect.The nano enzyme adhesive bandage, including adhesive plaster and medical cotton adhere to nano enzyme on the medical cotton；The nano enzyme is the nano material with Mimetic Peroxidase.The present invention also provides a kind of preparation methods of nano enzyme adhesive bandage.The present invention also provides a kind of application methods of nano enzyme adhesive bandage, including：By bacterial suspension H2O2It is coated in wound surface, nano enzyme adhesive bandage is then attached to wound surface.The adhesive bandage of the present invention can be by H2O2Efficiently catalysis is the hydroxyl radical free radical with strong oxidizing property, greatly improves H2O2Bactericidal property, reduce H2O2Use concentration, therefore avoid H2O2Toxic side effect.
Description
Technical field
The invention belongs to adhesive bandage technical fields, and in particular to a kind of nano enzyme adhesive bandage, preparation method and application method.
Background technology
Adhesive bandage is mainly made of plane adhesive plaster, water absorption pad and strippable coating, has the function of hemostasis, shield wound.It stops
Blood effect mainly acts on forming pressure hemostasis by the stickup of plane adhesive plaster, and by the water sorption of water absorption pad to absorb exudate
To keep wound relatively dry, promotion Wound healing.In addition, the water absorption pad of adhesive bandage can also add the drug with antibacterial activity
Such as：Benzalkonium chloride can effectively prevent the infection of wound.Based on above-mentioned characteristic, it is shallow, clean whole that adhesive bandage can be used for more table
Together, bleeding is few and the minor cut or wound that need not suture again.Since it is reliably excellent with easy to use, easy to carry and curative effect
Point, adhesive bandage have become people's common surgical medicine in daily life.
Although drug used at present has excellent antibacterial activity, a series of side effects, such as anaphylaxis can be caused
Rhinitis, rhinitis medicamentosa, nasosinusitis etc. cause great trouble for the health of human body.In addition, current adhesive bandage only has
Hemostasis, anti-infectious effect can not promote the breeding of wound cells, or even have very strong poison to the cell of wound circumference
Effect.In order to avoid current adhesive bandage toxic side effect and expand its function, provide it is a kind of it is simple for process, bio-compatibility is good
Well, production cost is relatively low and with multi-functional adhesive bandage, is a significant challenge.
Nano enzyme refers to the catalytic activity that nano material has similar native enzyme, and it is anti-can to be effectively catalyzed various biochemistries
It answers.So far, the activity of native enzyme can be simulated by having had more than 50 kinds of nano material to be reported, such as：Glucose oxidase, mistake
Hydrogen oxide enzyme, peroxidase, glutathione peroxidase etc..Since these nano enzymes are compared with native enzyme, it has system
It is standby it is simple, cheap, stability is good, the advantages that can enduring harsh reaction condition and being easy to mark and modify, therefore quilt
It is widely used in environmental monitoring, disease detection, contaminant degradation, neuro-protective etc..Based on its good bio-compatibility
And catalytic capability, nano enzyme will also have huge development space in adhesive bandage field.
H2O2It is common wound disinfection agent, this is because it, which can be decomposed, generates the hydroxyl radical free radical with strong oxidizing property, from
And destroy the active component of bacterium.But it is this to generate the less efficient of hydroxyl radical free radical；It is past in order to reach effective antibacterial effect
Toward the H that can use high concentration2O2, this is easy to cause immunogenicity and inflammation again, or even can delay the healing of wound.Nano enzyme is
Nanocatalyst with similar native enzyme, has gathered the stabilization and economy of the excellent catalytic capability of native enzyme and nano material
Double dominant, be widely used to the fields such as detection, environmental treatment and biomedicine.
Invention content
The purpose of the present invention is to solve the breedings that the drug that existing adhesive bandage uses cannot promote wound cells, and
And the cell of wound circumference has the problem of very strong toxic effect, and a kind of nano enzyme adhesive bandage, preparation method and user are provided
Method.
Present invention firstly provides a kind of nano enzyme adhesive bandage, including adhesive plaster and medical cotton, adheres on the medical cotton and receive
Rice enzyme；
The nano enzyme is the nano material with Mimetic Peroxidase.
Preferably, the nano material of the Mimetic Peroxidase is graphene quantum dot, ceria, nitrogen carbide
Or nitrogen carbide and gold nano grain compound.
Preferably, the preparation method of the nitrogen carbide and gold nano grain compound, including：
Gold chloride and glutamic acid are added in ethylene glycol, nitrogen carbide nanometer sheet is then added and are stirred at room temperature, carbon is obtained
Change nitrogen and gold nano grain compound.
Preferably, the size of the nitrogen carbide nanometer sheet is 200-300nm, thickness 1-3nm.
Preferably, the volume (μ L) of the gold chloride：The quality (mg) of glutamic acid：The quality of nitrogen carbide nanometer sheet
(mg) it is 100：50：30.
Preferably, a concentration of 18-25mg/mL of the gold chloride.
Preferably, the mixing time is 12-16 hours.
The present invention also provides a kind of preparation method of nano enzyme adhesive bandage, this method includes：
Nanometer enzyme aqueous solution, is added to the medical cotton of square by the medical cotton of fixed square on negative pressure leaching device
On, medical cotton is passed it through by filtering, the repetition process is fully absorbed by cotton fabric up to nano enzyme, is then taken out medical
Cotton, it is dry, the nano enzyme medical cotton after drying is fixed on adhesive plaster, nano enzyme adhesive bandage is obtained.
Preferably, a concentration of 0.2-2mg mL of the nanometer enzyme aqueous solution-1。
The present invention also provides a kind of application methods of nano enzyme adhesive bandage, including：
By bacterial suspension H2O2It is coated in wound surface, nano enzyme adhesive bandage is then attached to wound surface.
Beneficial effects of the present invention
Present invention firstly provides a kind of nano enzyme adhesive bandage, including adhesive plaster and medical cotton, adheres on the medical cotton and receive
Rice enzyme；The nano enzyme is the nano material with Mimetic Peroxidase.It compares with the prior art, wound of the invention can
Patch can be by H2O2Efficiently catalysis is the hydroxyl radical free radical with strong oxidizing property, greatly improves H2O2Bactericidal property, reduce
H2O2Use concentration, therefore avoid H2O2Toxic side effect.The nano enzyme adhesive bandage not only can effectively prevent wound sense
Dye, accelerates the healing of wound, while the nano enzyme adhesive bandage bio-compatibility is good, without apparent toxic side effect；And not
Cause any undesirable inflammatory reaction.
The present invention also provides the preparation method of a kind of nanometer of adhesive bandage, nano enzyme adhesive bandage preparation is simple, cost is relatively low,
It is significant in efficacy.
Description of the drawings
Fig. 1 is that the present invention detects nanometer enzymatic H using terephthalic acid (TPA) as fluorescence probe2O2Generate the ability of OH
Curve graph；
Fig. 2 is that the nano enzyme of the present invention improves H2O2Antibacterial ability curve graph；
Fig. 3 is the picture for the nano enzyme adhesive bandage that the embodiment of the present invention 2 and 5 is prepared.
Fig. 4 is therapeutic effect photo of the nano enzyme adhesive bandage to artificial wound of the present invention.
Fig. 5 is that the nano enzyme adhesive bandage of the present invention treats the histologic analysis picture after artificial wound.
Specific implementation mode
Present invention firstly provides a kind of nano enzyme adhesive bandage, including adhesive plaster and medical cotton, adheres on the medical cotton and receive
Rice enzyme；
The nano enzyme is the nano material with Mimetic Peroxidase, preferably graphene quantum dot, titanium dioxide
Cerium, nitrogen carbide or nitrogen carbide and gold nano grain compound.
According to the present invention, the preparation method of the nitrogen carbide and gold nano grain compound, by deposition-precipitation legal system
It is standby, including：
Gold chloride and glutamic acid are added in ethylene glycol, nitrogen carbide nanometer sheet is then added and is stirred at room temperature, by centrifuging,
Milli-Q water is used in combination three times, obtains nitrogen carbide and gold nano grain compound.
According to the present invention, nitrogen carbide block is prepared the nitrogen carbide nanometer sheet by excusing from death, the side
Method is specially：Melamine is placed in alumina crucible, 2h is calcined at 600 DEG C, you can the nitrogen carbide powder of yellow is obtained, it will
Obtained blocky 50mg nitrogen carbides powder is dispersed in the 5M HCl solution of 25mL and 1h is stirred at room temperature, and then, solution is mixed
Object is filtered, washed, and nitrogen carbide aqueous solution is then ultrasonically treated 4h under conditions of power 300W, and finally, ultrasound is obtained
Mixture is centrifuged in 3000rpm to remove remaining unstripped nitrogen carbide, obtains nitrogen carbide nanometer sheet；The nitrogen carbide is received
Rice piece is preferably dimensioned to be 200-300nm, thickness 1-3nm；The volume (μ L) of the gold chloride：The quality of glutamic acid
(mg)：The quality (mg) of nitrogen carbide nanometer sheet is preferably 100：50：30；The concentration of the gold chloride is preferably 18-25mg/
ML, more preferably 22.5mg/mL；The mixing time is preferably 12-16 hours.The centrifugal speed is preferably 8000-
10000rpm。
The present invention also provides a kind of preparation method of nano enzyme adhesive bandage, this method includes：
Nanometer enzyme aqueous solution, is added to the medical cotton of square by the medical cotton of fixed square on negative pressure leaching device
On, medical cotton is passed it through by filtering, the repetition process is fully absorbed by cotton fabric up to nano enzyme, is then taken out medical
Cotton, it is dry, the nano enzyme medical cotton after drying is fixed on adhesive plaster, nano enzyme adhesive bandage is obtained.The nano enzyme is water-soluble
The concentration of liquid is preferably 0.2-2mg mL-1, more preferably 1-1.5 mg mL-1；Drying temperature is preferably 30-60 DEG C, when dry
Between preferably 10-24 hour.
The present invention also provides a kind of application methods of nano enzyme adhesive bandage, including：
By bacterial suspension H2O2It is coated in wound surface, nano enzyme adhesive bandage is then attached to wound surface.
Further detailed description done to the present invention with reference to specific embodiment, involved in embodiment to raw material be
It is commercially available.
The preparation of embodiment 1 nitrogen carbide and gold nano grain compound
Melamine is placed in alumina crucible, 2h is calcined at 600 DEG C, you can the nitrogen carbide powder of yellow is obtained, it will
Obtained blocky 50mg nitrogen carbides powder is dispersed in the 5M HCl solutions of 25mL and is stirred at room temperature 1h, then by solution mixture
It is filtered, washed, then nitrogen carbide aqueous solution is ultrasonically treated 4h under conditions of power 300W, finally, the mixing that ultrasound is obtained
Object is centrifuged in 3000rpm to remove remaining unstripped nitrogen carbide, obtains nitrogen carbide nanometer sheet；
The gold chloride (22.5mg/mL) of 100 μ L and 50 milligrams of glutamic acid are added in 40 milliliters of ethylene glycol, then by 30
Nitrogen carbide nanometer sheet prepared by milligram is add to the above mixed solution and is stirred at room temperature overnight, and finally, passes through centrifugation
(8000rpm) and with milli-Q water three times, obtain nano enzyme nitrogen carbide and gold nano grain compound.
The preparation of 2 nano enzyme adhesive bandage of embodiment
The medical cotton of fixed square on negative pressure leaching device, the preparation of embodiment 1 nitrogen carbide is answered with gold nano grain
Close nanometer enzyme aqueous solution (the 1mg mL of object-1) it is added to the medical cotton of square, medical cotton is passed it through by filtering, repeats the mistake
Cheng Zhizhi nano enzymes are fully absorbed by cotton fabric, are then taken out the medical cotton and are allowed it dry 12 at 40 DEG C of vacuum drying chamber
A hour, it is nano enzyme adhesive bandage that the nano enzyme medical cotton after drying, which is fixed on adhesive plaster,.
In the photo such as Fig. 3 for the nano enzyme adhesive bandage that embodiment 2 is prepared shown in B figures, the sample of aubergine is in figure
For nitrogen carbide and gold nano grain compound, it was demonstrated that nano enzyme adhesive bandage has successfully been prepared in the present invention.
The preparation of 3 nano enzyme adhesive bandage of embodiment
The medical cotton of fixed square on negative pressure leaching device, by graphene quantum dot aqueous solution (1.5mg mL-1) plus
To the medical cotton of square, medical cotton is passed it through by filtering, repeats the process until nano enzyme is fully inhaled by cotton fabric
It receives, then take out the medical cotton and allows its dry 12 hour at 40 DEG C of vacuum drying chamber, the nano enzyme after drying is medical
It is nano enzyme adhesive bandage that cotton, which is fixed on adhesive plaster,.
The preparation of 4 nano enzyme adhesive bandage of embodiment
The medical cotton of fixed square on negative pressure leaching device, by cerium dioxide nano enzyme aqueous solution (1mg mL-1) plus
To the medical cotton of square, medical cotton is passed it through by filtering, repeats the process until nano enzyme is fully inhaled by cotton fabric
It receives, then take out the medical cotton and allows its dry 12 hour at 40 DEG C of vacuum drying chamber, the nano enzyme after drying is medical
It is nano enzyme adhesive bandage that cotton, which is fixed on adhesive plaster,.
The preparation of 5 nano enzyme adhesive bandage of embodiment
The medical cotton of fixed square on negative pressure leaching device, by nitrogen carbide nanometer enzyme aqueous solution (1mg mL-1) be added to
The medical cotton of square passes it through medical cotton by filtering, and the repetition process is fully absorbed by cotton fabric up to nano enzyme,
It then takes out the medical cotton and allows its dry 12 hour at 40 DEG C of vacuum drying chamber, the nano enzyme medical cotton after drying is consolidated
Be scheduled on adhesive plaster is nano enzyme adhesive bandage.
In the photo such as Fig. 3 for the nano enzyme adhesive bandage that embodiment 5 is prepared shown in A figures, the sample of yellow is in figure
Nitrogen carbide, it was demonstrated that nano enzyme adhesive bandage has successfully been prepared in the present invention.
Performance test is done to the nano enzyme and nano enzyme adhesive bandage of the present invention below.
1, nano enzyme improves H as catalyst2O2OH abilities are generated to investigate
Nanometer enzymatic H2O2Hydroxyl radical free radical is converted to, the measurement of hydroxyl radical free radical (OH) is based on it and to benzene two
Reaction between formic acid (TA), TA itself do not have fluorescence, but can capture OH and generate and have fluorescence about 435nm at
2- hydroxyterephthalic acids (TAOH).Nano enzyme, TA, H prepared by embodiment 12O2The phosphoric acid for being added separately to pH 7.4 is slow
In fliud flushing, and mixed solution is incubated 12 hours at 37 DEG C, then surveys its fluorescence.In addition, nano enzyme and TA, H2O2With TA conducts
Check experiment.
Fig. 1 is that the present invention detects nanometer enzymatic H using terephthalic acid (TPA) as fluorescence probe2O2Generate the ability of OH
Curve graph, wherein figure A is nanometer enzymatic H2O2The schematic diagram of hydroxyl radical free radical is converted to, figure B is H2O2With and without
Fluorogram after being reacted with terephthalic acid (TPA) in the presence of nano enzyme, figure C are H2O2With nano enzyme+H2O2Generate the ability of OH
Fluorogram, from figure 1 it appears that nano enzyme can be effectively by H2O2Hydroxyl radical free radical is converted to, efficiency is independent
H2O2Nearly 9 times.
2, nano enzyme improves H2O2Anti-microbial property investigate
By on agar medium Escherichia coli (E.coli) and staphylococcus aureus (S.aureus) be transferred to LB liquid
In body culture medium, and it is placed in bacterium shaking table and 12h is cultivated at 37 DEG C with rotating speed 170rpm.Then, by the way that bacterium is collected by centrifugation
LB culture mediums be used in combination that bacterium is diluted to 106CFU mL-1.Then the nano enzyme and H it prepared with embodiment 12O2It is common to be incubated
After cultivating 8h, its OD is surveyed600nm。
Fig. 2 is that the nano enzyme of the present invention improves H2O2Antibacterial ability curve graph, wherein figure A be various concentration H2O2Having
Or that, to the fragmentation effect of E.coli, figure B is not various concentration H in the presence of nano enzyme2O2Exist with and without nano enzyme
Under to the fragmentation effect of S.aureus, figure C is the nano enzyme of various concentration respectively to the fragmentation effect of E.coli and S.aureus,
It is toxicity research of the nano enzyme to mouse normal cell NIH3T3 to scheme D, figure it is seen that in the presence of nano enzyme, H2O2
The anti-microbial property of E.coli and S.aureus is greatly enhanced, sees Fig. 2A and 2B, with the help of nano enzyme, H2O2Antibacterial
Required concentration declines two orders of magnitude, avoids high concentration H2O2Toxic side effect.As a comparison, in no H2O2In the presence of, nanometer
Enzyme does not have apparent killing ability (Fig. 2 C) to E.coli and S.aureus.In addition, seeing Fig. 2 D, the nano enzyme is thin to NIH3T3
Born of the same parents do not have apparent toxicity, show its good bio-compatibility.
3, artificial wound model is built, the therapeutic effect of nano enzyme adhesive bandage is investigated
First, depilation processing is carried out to the back of experiment mice using depilatory cream.Then, with the operation after sterilizing, disinfection
It cuts the mouse back after depilation and cuts wound.There is the mouse of wound to be divided into four groups at back, respectively Blank adhesive bandages are (right
According to group), H2O2, nano enzyme adhesive bandage, nano enzyme adhesive bandage (embodiment 2)+H2O2Group.Fig. 4 is seen, from fig. 4, it can be seen that nanometer
Enzyme adhesive bandage+H2O2The wound of group processing has apparent knot over the course for the treatment of without there is any erythema and oedema
Scab, and start to become mildewed.As a comparison, there is different degrees of erythema and oedema in the wound of other 3 groups of mouse, or even has biology
The formation of envelope.Importantly, the H used in experimentation2O2A concentration of 10 μM, than the concentration (166mM- usually used
It 1M) wants much lower, greatly reduces H2O2Toxic side effect.Further, since wound location has the H of higher concentration2O2, nanometer
Enzyme itself also shows wound tissue certain therapeutic effect.
4, the histologic analysis of nano enzyme adhesive bandage treat wound
After nano enzyme adhesive bandage (embodiment 2) treat wound 3 days, the skin histology of mouse wound is extracted, and is fixed on
It in 10% paraformaldehyde, is then embedded in paraffin, about 4 μm of slice thickness, finally hematoxylin-eosin (H＆E) is used to dye
It is sliced carry out histologic analysis to it, observe the healing state of skin histology and whether has the generation of inflammatory reaction.
As shown in figure 5, the nano enzyme adhesive bandage that Fig. 5 is the present invention treats the histologic analysis picture after artificial wound, from
Fig. 5 can be seen that through nano enzyme and H2O2The skin histology of mouse wound after treatment shows epidermis that is complete and thickening.
In addition, the sign of inflammation is also not observed in wound location.And other groups of picture is shown, not only there is certain inflammatory cell
Infiltration and also skin histology all also have certain damage.
Claims (10)
1. a kind of nano enzyme adhesive bandage, including adhesive plaster and medical cotton, which is characterized in that adhere to nano enzyme on the medical cotton；
The nano enzyme is the nano material with Mimetic Peroxidase.
2. a kind of nano enzyme adhesive bandage according to claim 1, which is characterized in that the Mimetic Peroxidase is received
Rice material is graphene quantum dot, ceria, nitrogen carbide or nitrogen carbide and gold nano grain compound.
3. a kind of nano enzyme adhesive bandage according to claim 2, which is characterized in that the nitrogen carbide and gold nano grain
The preparation method of compound, including：
Gold chloride and glutamic acid are added in ethylene glycol, nitrogen carbide nanometer sheet is then added and are stirred at room temperature, nitrogen carbide is obtained
With gold nano grain compound.
4. a kind of nano enzyme adhesive bandage according to claim 3, which is characterized in that the size of the nitrogen carbide nanometer sheet
For 200-300nm, thickness 1-3nm.
5. a kind of nano enzyme adhesive bandage according to claim 3, which is characterized in that the volume (μ L) of the gold chloride：
The quality (mg) of glutamic acid：The quality (mg) of nitrogen carbide nanometer sheet is 100：50：30.
6. a kind of nano enzyme adhesive bandage according to claim 3, which is characterized in that a concentration of 18- of the gold chloride
25mg/mL。
7. a kind of nano enzyme adhesive bandage according to claim 3, which is characterized in that the mixing time is that 12-16 is small
When.
8. a kind of preparation method of nano enzyme adhesive bandage according to claim 1, which is characterized in that this method includes：
The medical cotton of fixed square, nanometer enzyme aqueous solution is added on the medical cotton of square on negative pressure leaching device, is led to
It crosses suction filtration and passes it through medical cotton, the repetition process is fully absorbed by cotton fabric up to nano enzyme, then takes out medical cotton, is done
It is dry, the nano enzyme medical cotton after drying is fixed on adhesive plaster, nano enzyme adhesive bandage is obtained.
9. a kind of preparation method of nano enzyme adhesive bandage according to claim 8, which is characterized in that the nano enzyme water
A concentration of 0.2-2mg mL of solution-1。
10. a kind of application method of nano enzyme adhesive bandage described in claim 1, which is characterized in that including：By bacterial suspension
H2O2It is coated in wound surface, nano enzyme adhesive bandage is then attached to wound surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810399824.9A CN108420803A (en) | 2018-04-28 | 2018-04-28 | Nano enzyme adhesive bandage, preparation method and application method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810399824.9A CN108420803A (en) | 2018-04-28 | 2018-04-28 | Nano enzyme adhesive bandage, preparation method and application method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041387A (en) * | 2021-03-17 | 2021-06-29 | 复旦大学附属中山医院 | Antibacterial dressing for blocking drug resistance propagation |
| CN113599506A (en) * | 2021-05-31 | 2021-11-05 | 长沙理工大学 | Platinum nanoenzyme/glucose oxidase @ hyaluronic acid composite antibacterial material and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107754004A (en) * | 2017-10-11 | 2018-03-06 | 泰山医学院 | A kind of adhesive bandage and its preparation and application |
-
2018
- 2018-04-28 CN CN201810399824.9A patent/CN108420803A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107754004A (en) * | 2017-10-11 | 2018-03-06 | 泰山医学院 | A kind of adhesive bandage and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| ZHENZHEN WANG ET AL.: ""Activation of biologically relevant levels of reactive oxygen species by Au/g-C3N4 hybrid nanozyme for bacteria killing and wound disinfection"", 《BIOMATERIALS》 * |
| 黄燕燕等: ""纳米酶在疾病治疗中的最新进展"", 《生物化学与生物物理进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041387A (en) * | 2021-03-17 | 2021-06-29 | 复旦大学附属中山医院 | Antibacterial dressing for blocking drug resistance propagation |
| CN113041387B (en) * | 2021-03-17 | 2022-04-08 | 复旦大学附属中山医院 | Antibacterial dressing for blocking drug resistance propagation |
| CN113599506A (en) * | 2021-05-31 | 2021-11-05 | 长沙理工大学 | Platinum nanoenzyme/glucose oxidase @ hyaluronic acid composite antibacterial material and preparation and application thereof |
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