CN108414330B - Improved structure of sample dyeing module for automatic slice dyeing machine - Google Patents
Improved structure of sample dyeing module for automatic slice dyeing machine Download PDFInfo
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- CN108414330B CN108414330B CN201810210274.1A CN201810210274A CN108414330B CN 108414330 B CN108414330 B CN 108414330B CN 201810210274 A CN201810210274 A CN 201810210274A CN 108414330 B CN108414330 B CN 108414330B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- Immunology (AREA)
- Pathology (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
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Abstract
The invention relates to an improved structure of a sample dyeing module of an automatic slice dyeing machine, which comprises a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell; the bottom of the shell is provided with a heating device; the cooling fan is arranged below the shell, and a groove with a curved central line is arranged at the bottom of the shell; the control unit is respectively connected with the driving device, the drain valve, the heating device and the cooling fan in a signal mode. This technical scheme is through trilateral deep bead that is provided with in casing bottom to be provided with the central line on casing bottom surface and be curved recess, and do not set up the one side of deep bead in the bottom of casing through the recess opening, make cooling fan blow the air current flow direction and the velocity of flow of the bottom of casing all increase, improved cooling fan's cooling effect, improve the histocyte form of biological sample after the antigen retrieval, improve pathological examination's accuracy and reliability.
Description
Technical Field
The invention belongs to the field of medical detection instruments, particularly relates to the technical field of immunohistochemistry and immunocytochemistry dyeing, and more particularly relates to an improved structure of a sample dyeing module for an automatic slide staining machine.
Background
Immunohistochemistry (IHC) is a new technology developed by combining the theory and technology of immunology on histochemical methods, and combines the theory and method of the subjects of immunology, histology and biochemistry, etc. The core of immunology, namely the principle of specific combination of antigen and antibody, is utilized, the antigen is tracked by using the antibody marked with enzyme, metal ion, isotope and the like, a display agent marked on the specific antibody combined with the antigen shows a certain color through chemical reaction, and the dyeing result is observed by means of a microscope, a fluorescence microscope and an electron microscope, so that the purpose of detecting the antigen is achieved.
Immunohistochemistry has a long history, and since 1940 Coons established immunofluorescence techniques to detect the corresponding antigens in frozen sections, there has been 73 history to date. In 1965, the immunohistochemical technique was first established in China, but the method was not widely applied to pathological diagnosis until the early 90 s in the 20 th century. The technique goes through 4 stages of development: establishing and developing an immunohistochemical technology; a popularization stage; clinical pathological diagnosis and application stage; quality control, normalization and standardization phases. Today, without the aid of immunohistochemistry, many tumors are poorly diagnosed and classified. Meanwhile, the wide application of immunohistochemistry in clinical pathological diagnosis also promotes the rapid development of immunohistochemical technology.
Since 1950, Journal of Histochemistry & Cytochemistry published a large number of documents of new technologies related to histomorphosis, such as enzyme-labeled immunohistochemistry, avidin biotin detection system, casein signal amplification system, and the like. All of these valuable findings have prompted the development of clinical and basic biomedical research worldwide. Immunohistochemistry has been widely used and accepted, and has become an indispensable link in pathological diagnosis. Immunohistochemistry not only improves the accuracy of pathological diagnosis, but also permeates basic and clinical subjects, and plays an immeasurable role in discussing etiology and pathogenesis of diseases and scientific research work.
The main steps in the immunohistochemical staining process include: baking, deparaffinization, hydration, antigen retrieval, or peroxidase blocking (optionally added), primary, secondary or tertiary antibody (optionally added), DAB or hematoxylin (optionally added), and all rinses.
With the development of science and technology, various advanced technologies are also gradually applied to the immunohistochemical automatic staining process to replace manual staining.
In each step of immunohistochemical staining, the most difficult is the antigen retrieval process, because the most common fixative for tissue fixation is formaldehyde, which will cross-link with proteins during tissue fixation to form a methylene-bridged cross-linked structure, thereby shielding the antigenic determinants in the tissue. Professor litholytica found that heating disrupted the protein cross-linking by formaldehyde fixation, re-exposing the masked antigenic determinants, and allowing binding to the corresponding antibodies.
At present, the antigen retrieval mode and the reagent type selection in the manual immunohistochemical staining process are flexible, and the common heat-guided antigen retrieval modes comprise: high pressure, water boiling, microwave, water bath, etc.; commonly used antigen retrieval solutions are citric acid (pH6.0), EDTA (pH9.0), Tris-HCl (pH 9.0-10). Different laboratories have different links such as detection personnel, selection of heating devices, control of heating temperature and time and the like, so that the immunohistochemical staining result inevitably has difference.
In order to overcome the difference of artificial dyeing results, an immunohistochemical automatic dyeing system is developed, and in recent years, full-automatic biochemical analyzers with different models and functions are in succession, so that the working efficiency and the detection accuracy are greatly improved. However, the heating temperature of the antigen repairing solution is lower than 100 ℃ for antigen repairing in the current full-automatic staining instrument in the market, and although manufacturers expect high-temperature repairing by adding high-boiling-point substances into the antigen repairing solution, in the antigen repairing process, after heating, the glass slide needs to be washed after cooling, in order to increase the cooling rate, the cooling speed is increased by a fan, but when the fan cools, the drying phenomenon occurs on the surface of a tissue slice on the glass slide, and the effect of combining with a corresponding antibody is influenced.
Disclosure of Invention
The invention aims to provide an improved structure of a sample dyeing module for an automatic slide staining machine, which aims to solve the problems that the cooling time is long after the antigen retrieval is finished, and the pathological diagnosis is influenced due to the change of the tissue cell morphology caused by the long cooling time in the prior art.
The invention is realized by the following technical scheme:
an improved structure of a sample dyeing module for an automatic slice dyeing machine comprises a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell;
the output shaft of the driving device transmits power to the connecting shaft so as to control the opening and closing of the shell cover;
a drainage tank is arranged in the shell and is communicated with each liquid storage tank; a drain hole is formed in the shell, one end of the drain hole is communicated with the drain groove, and the other end of the drain hole is opened on the outer side of the shell and is connected with a drain valve;
the heating device is arranged at the bottom of the shell;
a cooling fan disposed below the housing;
wind shields extending downwards are arranged on three peripheries of the periphery of the bottom of the shell; at least more than two grooves are formed in the outer surface of the bottom of the shell, and one end of each groove is communicated with the periphery of the bottom of the shell, which is not provided with the wind shield; the central line of each groove is a curve;
and the control unit is respectively in signal connection with the driving device, the drain valve, the heating device and the cooling fan.
A support platform for placing a glass slide is arranged in each liquid storage tank;
and the slicing frame is arranged on the supporting platform and is provided with clamping devices which correspond to the liquid storage tanks one to one.
A drainage plate and a waste liquid groove are arranged on the inner side of the shell cover, the drainage plate is obliquely arranged, and one side, close to the waste liquid groove, of the drainage plate is lower;
the waste liquid groove is communicated with the outer surface of the shell cover.
The output shaft of the driving device and the connecting shaft are in any one of belt transmission, chain transmission, gear transmission or hydraulic transmission.
The driving device is a motor, and the motor is fixed on the shell.
And a temperature sensor is arranged in the shell and is in signal connection with the control unit.
And a wind shield is arranged between two adjacent module grooves.
When each groove extends from one end of the inner side of the bottom of the shell to the other end of the bottom of the shell, the depth of each groove is gradually increased.
The axial centerline of the cooling fan coincides with the housing bottom centerline.
The invention has the beneficial effects that:
this technical scheme is through trilateral deep bead that is provided with in casing bottom to be provided with the central line on casing bottom surface and be curved recess, and do not set up the one side of deep bead in the casing bottom through the recess opening, make cooling fan blow the air current flow direction and the velocity of flow of casing bottom and all increase, improved cooling fan's cooling effect.
On the other hand, according to the technical scheme, the grooves are arranged into the structure with the depth gradually increasing from inside to outside, so that the cross sectional area of the grooves is gradually increased, the air flow is increased, the air cooling effect is accelerated, the soaking time of the antigen repairing liquid is shortened, the tissue cell morphology of the biological sample after antigen repairing is improved, and the accuracy and the reliability of pathological diagnosis are improved.
Drawings
FIG. 1 is a schematic view of a dyeing module according to the present invention;
FIG. 2 is a front view of a staining module of the invention;
FIG. 3 is a bottom view of the staining module with the fan removed according to the present invention.
Description of the reference numerals
The device comprises a shell 1, a shell cover 2, a flow guide plate 3, a sealing ring 4, a connecting shaft 5, a supporting table 6, a wind shield 7, a slicing frame 8, an output shaft 9, a limiter 10, a motor 11, a fan 12, a drain hole 13, a drain valve 14, an electric heating plate 15, a wind shield 20 and a groove 21.
Detailed Description
The technical solutions of the present invention are described in detail below by examples, and the following examples are only exemplary and can be used only for explaining and explaining the technical solutions of the present invention, but not construed as limiting the technical solutions of the present invention.
As shown in fig. 1, a housing 1 with a housing cover 2 is provided, the housing cover 2 is hinged to the housing 1 through a connecting shaft 5, and the housing cover can be opened or closed through the rotation of the connecting shaft, so that the dyeing module can be operated in each immunohistochemical process.
The dyeing module device belongs to a part of an automatic slice dyeing machine, and in the technical scheme of the application, only the dyeing module is improved, and the rest parts of the automatic slice dyeing machine still belong to the prior art, so that the structure of the automatic slice dyeing machine is not explained in the application.
The specific structure of the dyeing module device is as shown in fig. 1, a plurality of liquid storage tanks for storing reagents are arranged in a shell 1, the liquid storage tanks are arranged side by side, a drainage tank is arranged on one side opposite to the liquid storage tanks in the shell, and each liquid storage tank is communicated with the drainage tank, so that liquid in each liquid storage tank can be quickly drained through the drainage tank. The bottom of the shell is provided with a drain hole opposite to the drain tank, as shown in fig. 3, the drain hole is connected with a drain valve, the drain valve is in signal connection with a control unit, the control unit outputs an opening signal to the drain valve, and the drain valve opens the drain valve after receiving the opening signal, so that liquid in the drain tank is drained.
A supporting platform 6 for placing the glass slide is arranged in each liquid storage tank, the supporting platform 6 is used for placing a slicing frame 8, and clamping devices which correspond to the liquid storage tanks one by one are arranged on the slicing frame 8. In the present application, the clamping device is prior art.
As shown in fig. 2, a driving device is disposed on a side surface of the housing, in this embodiment, the driving device is a motor 11, an output shaft 9 of the motor 11 is connected to the connecting shaft through a belt, a chain, a gear, or hydraulic pressure, and when the motor operates, the connecting shaft is driven to rotate, so as to open or close the housing cover.
And the shell body 1 and the shell cover 2 are provided with a limiter 10 which is used for controlling the opening and closing amount of the shell cover, and the shell cover is provided with a sealing ring 4 which is used for sealing with the shell body.
The inner side of the shell cover is provided with a drainage plate 3 and a waste liquid groove, and the drainage plate is obliquely arranged and one side close to the waste liquid groove is lower; so that the condensed water flows to the waste liquid tank through the liquid guide plate.
The waste liquid tank is communicated with the outer surface of the shell cover and discharges condensed water in the waste liquid tank out of the shell.
As shown in fig. 3, a heating device is disposed on the dyeing module, in this embodiment, the heating device is disposed at the bottom of the housing, and the heating device is an electric heating plate 15, the heating device can be disposed on the inner surface of the bottom of the housing and can be attached to the outer surface of the bottom of the housing, the heating device is in signal connection with the control unit, the control unit controls the heating device to heat or stop heating according to a set program, and specifically controls when the heating device is turned on or off by signals such as a temperature sensor disposed in the housing and a contact sensor disposed between the housing and the housing cover.
As shown in fig. 2, the dyeing module includes a cooling device, in this application, a cooling fan 12 is disposed below the housing, and the cooling air blown by the cooling fan is directed to the bottom of the housing.
In the technical scheme of this application, be provided with downwardly extending deep bead 20 in three peripheries in the surface of casing bottom in the periphery, overall structure is that three deep bead and casing bottom form a box-shaped structure that lacks a side, and the cooling air that the fan blew off is just to the casing bottom.
On the surface of casing bottom, be provided with a plurality of recesses 21, the one end of every recess all link up with the one side of the casing bottom that is not provided with the deep bead to the central line of every recess is the curve, such groove structure for blow in the cooling air of casing bottom surface, have partly through the guide of recess, and flow through casing bottom surface with higher speed, because the increase of velocity of flow, improve cooling speed naturally.
Simultaneously, this application every the recess is all from being located the one end of the inboard of casing bottom to when the other end of casing bottom extends, the degree of depth of recess increases gradually. The specific structure is that the groove and one end of one side edge of the bottom of the shell, which is not provided with the wind shield, are the outer side end of the groove, and the other end of the groove is called as the inner side end of the groove because other positions of the bottom of the shell are arranged.
This technical scheme is through setting up the recess to the structure that the degree of depth from inside to outside gradually increases for the cross-sectional area of recess gradually increases, improves air flow flux, accelerates the cooling effect of air.
The advantage of this technical scheme is, in current technical scheme, cooling fan when operation, directly blows the cooling air to the surface of casing bottom, and in order to avoid this cooling air can disturb adjacent dyeing module, consequently, all be provided with the deep bead around every casing bottom, and such structure can lead to the cooling air that blows to the casing bottom and the cooling air that is heated that reflects from the casing bottom to run relatively, and such result just makes new cooling air blow to the bottom of casing and is hindered and increase the flow time, and simultaneously, the cooling air that is heated increases dwell time because of being hindered, and this condition just in time influences the cooling effect.
This technical scheme is through canceling one side in the deep bead all around with casing bottom to set up the central line at the surface of casing bottom and be curved recess, blow the cooling air of casing bottom like this and carry out the heat exchange back with the casing bottom, the cooling air that is heated passes through the recess and flows out the casing bottom, and the flow direction is different with the flow direction of the cooling air that cooling fan blown in addition, has avoided hindering, consequently the cooling wind speed that improves the cooling effect.
A wind shield 7 is arranged between two adjacent liquid storage tanks in the shell to prevent adjacent glass slides from being influenced when air is blown.
As shown in fig. 3, the axial centerline of the cooling fan coincides with the housing bottom centerline; the cooling fan can uniformly distribute the cooling air blown out by the cooling fan to the bottom of the shell, so that the cooling uniformity of each liquid storage tank is ensured.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. An improved structure of a sample dyeing module for an automatic slice dyeing machine is characterized by comprising a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell;
the output shaft of the driving device transmits power to the connecting shaft so as to control the opening and closing of the shell cover;
a drainage tank is arranged in the shell and is communicated with each liquid storage tank; a drain hole is formed in the shell, one end of the drain hole is communicated with the drain groove, and the other end of the drain hole is opened on the outer side of the shell and is connected with a drain valve;
the heating device is arranged at the bottom of the shell;
a cooling fan disposed below the housing;
wind shields extending downwards are arranged on three peripheries of the periphery of the bottom of the shell; more than two grooves are formed in the outer surface of the bottom of the shell, and one end of each groove is communicated with the periphery of the bottom of the shell, which is not provided with the wind shield; the central line of each groove is a curve;
when each groove extends from one end of the inner side of the bottom of the shell to the other end of the bottom of the shell, the depth of each groove is gradually increased;
and the control unit is respectively in signal connection with the driving device, the drain valve, the heating device and the cooling fan.
2. The improved structure of the sample dyeing module for the automatic slide dyeing machine according to the claim 1, characterized in that, a supporting platform for placing the slide is arranged in each liquid storage tank;
the slicing frame is arranged on the supporting platform, and clamping devices which correspond to the liquid storage tanks one to one are arranged on the slicing frame.
3. The improved structure of the sample dyeing module for the automatic dyeing machine according to the claim 1, characterized in that the inner side of the shell cover is provided with a drainage plate and a waste liquid groove, the drainage plate is arranged obliquely, and one side close to the waste liquid groove is lower;
the waste liquid groove is communicated with the outer surface of the shell cover.
4. The improved structure of the sample dyeing module for the automatic slice dyeing machine as claimed in claim 1, characterized in that the output shaft of the driving device and the connecting shaft are driven by any one of belt transmission, chain transmission, gear transmission or hydraulic transmission.
5. The improved structure of the sample dyeing module for the automatic slice dyeing machine as claimed in claim 4, characterized in that the driving device is a motor, and the motor is fixed on the shell.
6. The improved structure of the sample dyeing module for the automatic slice dyeing machine as claimed in claim 1, characterized in that a temperature sensor is arranged in the housing, and the temperature sensor is in signal connection with the control unit.
7. The improvement of a sample dyeing module for an automatic slide dyeing machine according to claim 1, characterized in that the axial midline of said cooling fan coincides with the housing bottom midline.
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| IT202100018659A1 (en) * | 2021-07-15 | 2023-01-15 | Bioevo S R L | SYSTEM FOR STAINING SLIDES FOR MICROSCOPIC OBSERVATIONS, AND RELATED METHOD |
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| CN117571429B (en) * | 2023-10-13 | 2024-06-14 | 浙江莱阅病理诊断科技有限公司 | Immunohistochemical staining module and staining method |
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| CN2543056Y (en) * | 2002-05-20 | 2003-04-02 | 余向东 | Immunohistochemistry dyeing automatic controller |
| CN102999133A (en) * | 2012-10-12 | 2013-03-27 | 苏州征之魂专利技术服务有限公司 | Laptop cooling support |
| CN203964738U (en) * | 2014-07-03 | 2014-11-26 | 叶向南 | A kind of blowing heat abstractor |
| CN105571925B (en) * | 2016-03-09 | 2018-02-09 | 福州迈新生物技术开发有限公司 | The staining modules and its colouring method of biological sample on slide |
| CN205656054U (en) * | 2016-05-25 | 2016-10-19 | 塔里木大学 | Paraffin section statining jar with water conservancy diversion function |
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| IT202100018659A1 (en) * | 2021-07-15 | 2023-01-15 | Bioevo S R L | SYSTEM FOR STAINING SLIDES FOR MICROSCOPIC OBSERVATIONS, AND RELATED METHOD |
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