CN108410821B - Medical human body red blood cell culture method - Google Patents
Medical human body red blood cell culture method Download PDFInfo
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Abstract
The invention relates to the technical field of cell culture, in particular to a medical human body red blood cell culture method, which comprises a cell storage mechanism, a sealing mechanism, a supporting mechanism, a heat preservation mechanism, a dispersion treatment mechanism and a liquid separation control mechanism, wherein the cell storage mechanism is arranged on the cell storage mechanism; according to the invention, through the dispersion treatment mechanism and the liquid separation control mechanism, an operator can disperse the red blood cell liquid from the cell culture box to each collection plate according to experiment requirements, so that the preparation of a plurality of culture carriers is realized, and the operator can detect the states of the red blood cell liquid in different collection plates, thereby obtaining more accurate experiment data; and each collecting plate and the circulating pipe are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to each collecting plate; install heat preservation mechanism in the bottom of storing the culture box, can be so that to store the culture box and be in the constant temperature state in the observation process, red blood cell liquid can not supercool or overheated, the effectual activity of guaranteeing red blood cell.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a medical human body red blood cell culture method.
Background
Red blood cells, also called erythrocytes or red blood cells or erythrocytes, are the most abundant blood cells in blood. Red blood cells contain hemoglobin, and thus make blood red. Hemoglobin can be combined with oxygen in the air, so that the red blood cells can transport the oxygen absorbed into the alveoli to the tissues through the hemoglobin, and carbon dioxide metabolically produced in the tissues is also transported to the lungs through the red blood cells and is discharged out of the body.
The red blood cells are very important for human bodies, so that the research on the red blood cells is of great significance, when the red blood cells are cultured, the conventional culture mode mainly adopts a simple culture dish, and the method is inconvenient for transferring red blood cell liquid and cannot be used for quickly preparing a plurality of samples; the protection measures for the red blood cells are not enough, so that the red blood cells are easily influenced by the external temperature when being tested and observed. In view of the above, the invention provides a medical human body red blood cell culture method, which has the following characteristics:
(1) according to the medical human body red blood cell culture method, operators can disperse red blood cell liquid from the cell culture box to each collecting plate according to experiment requirements through the dispersion processing mechanism and the liquid separation control mechanism, so that preparation of a plurality of culture carriers is realized, manual transfer preparation is not needed, the operators can conveniently detect the states of the red blood cell liquid in different collecting plates, more accurate experiment data can be obtained, and the collecting plates and the circulating pipes are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to the collecting plates;
(2) according to the medical human body red blood cell culture method, the heat preservation mechanism is arranged at the bottom of the storage culture box, so that the storage culture box can be in a constant temperature state in the observation process, the red blood cell liquid cannot be supercooled or overheated, the activity of the red blood cells is effectively guaranteed, and the success rate of experiments is improved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a medical human body red blood cell culture method, which can enable an operator to disperse red blood cell liquid from a cell culture box to each collecting plate according to the experiment requirement through a dispersion treatment mechanism and a liquid separation control mechanism, thereby realizing the preparation of a plurality of culture carriers, and the operator can detect the states of the red blood cell liquid in different collecting plates, thereby obtaining more accurate experiment data; and each collecting plate and the circulating pipe are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to each collecting plate; install heat preservation mechanism in the bottom of storing the culture box, can be so that store the culture box and be in the constant temperature state in the observation process, red blood cell liquid can not supercool or overheated, the effectual activity of guaranteeing red blood cell has improved the success rate of experiment.
The technical scheme adopted by the invention for solving the technical problems is as follows: a medical human body red blood cell culture method comprises the following steps:
firstly, extracting and separating human red blood cells;
step two, placing the human body red blood cells prepared in the step one into an artificially prepared culture solution for culturing, wherein the composition and the content of the artificially prepared culture solution are the same as those of healthy human body blood without red blood cells;
step three, putting the culture solution added with the red blood cells in the step two into a culture instrument for culture;
the culture instrument comprises a cell storage mechanism, a sealing mechanism, a supporting mechanism, a heat preservation mechanism, a dispersion treatment mechanism and a liquid separation control mechanism; the bottom of the cell storage mechanism is provided with the supporting mechanism; the dispersion treatment mechanism is connected to the inside of the supporting mechanism in a sliding manner; the dispersion treatment mechanism is positioned at the bottom of the cell storage mechanism; the top of the cell storage mechanism is connected with the sealing mechanism in a matching way; the outer side wall of the supporting mechanism is connected with the liquid separation control mechanism; the bottom surface of the cell storage mechanism is provided with the heat preservation mechanism.
Specifically, the cell storage mechanism comprises a storage culture box, a first flow pipe, a second flow pipe and a third flow pipe, wherein the first flow pipe, the second flow pipe and the third flow pipe are communicated with the bottom surface of the storage culture box; the first flow pipe, the second flow pipe and the third flow pipe are distributed in a staggered and inclined manner, the distances between the first flow pipe, the second flow pipe and the third flow pipe and the side edges of the storage and culture box are gradually increased, and the lengths of the first flow pipe, the second flow pipe and the third flow pipe are gradually reduced; the red blood cell fluid can be discharged through the first flow pipe, the second flow pipe and the third flow pipe, so that the red blood cell fluid can be rapidly circulated without manual transfer.
Specifically, the sealing mechanism comprises a sealing cover plate and a sealing plug, the sealing plug is mutually matched and connected with the opening at the top of the storage culture box, the sealing cover plate is arranged at the top of the sealing plug, the sealing cover plate is of a transparent structure, and the area of the sealing cover plate is larger than that of the opening at the top of the storage culture box; can fill in the sealing plug and store in the culture box, seal the protection to storing the culture box, prevent that external environment from disturbing the growth of red blood cell to adopt transparent construction's sealed apron, the growth situation of the operating personnel observation cell of can being convenient for.
Specifically, the supporting mechanism comprises a supporting box and a limiting knob, the supporting box is arranged at the bottom of the storage culture box, sliding grooves are formed in the inner wall of the supporting box, the limiting knob is in threaded connection with two side walls of the supporting box, the supporting box is of a cavity structure, the cross section of the side of the supporting box is U-shaped, and at least three sliding grooves are formed in the inner wall of the supporting box from top to bottom; the storage culture box can be stably supported through the supporting mechanism, so that the stability of the cell storage mechanism and the dispersion treatment mechanism is ensured; the water supply mechanism can provide water into the heat preservation pipe, and the temperature of the culture solution in the storage culture box can be changed through the heat preservation pipe, so that cells are cultured and observed at a proper temperature.
The heat preservation mechanism comprises heat preservation pipes, connecting pipes, a water inlet joint, a water outlet joint and a water supply mechanism, wherein the heat preservation pipes are arranged on the bottom surface of the storage culture box, the heat preservation pipes are communicated with one another through the connecting pipes, one ends of the heat preservation pipes are connected with the water inlet joint, the other ends of the heat preservation pipes are connected with the water outlet joint, the water inlet joint and the water outlet joint are communicated to the water supply mechanism through guide pipes, the heat preservation pipes are distributed in a horizontal array shape, and the length of each heat preservation pipe is the same as the width of the storage culture box; the water supply mechanism can provide water into the heat preservation pipe, and the temperature of the culture solution in the storage culture box can be changed through the heat preservation pipe, so that cells are cultured and observed at a proper temperature.
Specifically, the water supply mechanism comprises a water tank, an electric heating rod and a circulating pump, one end of the circulating pump is connected with the water inlet joint through a guide pipe, the other end of the circulating pump is communicated to the inside of the water tank through a guide pipe, and the electric heating rod is installed inside the water tank; in order to realize the circulation supply of water with proper temperature.
Specifically, the dispersion treatment mechanism comprises a first collecting plate, a second collecting plate and a third collecting plate, the first collecting plate, the second collecting plate and the third collecting plate are all matched and connected with the sliding groove, a first feed port is arranged on the surface of the first collecting plate close to the side edge, the first feed port is arranged opposite to the bottom end of the first flow pipe, a second feed port is arranged on the surface of the second collecting plate close to the side edge, the second feed port is arranged opposite to the bottom end of the second flow pipe, a third feed port is arranged on the surface of the third collecting plate close to the side edge, the third feed port is arranged opposite to the bottom end of the third flow pipe, and the lengths of the first collecting plate, the second collecting plate and the third collecting plate are gradually reduced; in order to match with different flow tubes, the red blood cell liquid is collected in a dispersing way, so that an operator can observe and detect conveniently.
Specifically, guide rails are symmetrically arranged on the side walls of the first collecting plate, the second collecting plate and the third collecting plate, handles are arranged on the outer walls of the first collecting plate, the second collecting plate and the third collecting plate, the guide rails and the sliding grooves are mutually matched and connected, limiting holes are formed in the end portions of the guide rails, and the limiting holes are in threaded connection with the limiting knobs; an operator can insert or pull out the collecting plate by holding the handle by hand, so that the collecting plate is more convenient to operate.
Specifically, the liquid separation control mechanism comprises a first control knob, a second control knob and a third control knob, the first control knob is in threaded connection with the bottom end of the first flow pipe, the second control knob is in threaded connection with the bottom end of the second flow pipe, and the third control knob is in threaded connection with the bottom end of the third flow pipe; the flow process of the red blood cell fluid is controlled, and the operation is flexible and simple.
The invention has the beneficial effects that:
(1) according to the medical human body red blood cell culture method, operators can disperse the red blood cell liquid from the cell culture box to each collecting plate according to experiment requirements through the dispersion processing mechanism and the liquid separation control mechanism, so that preparation of a plurality of culture carriers is realized, manual transfer preparation is not needed, the operators can detect the states of the red blood cell liquid in different collecting plates, more accurate experiment data is obtained, and the collecting plates and the circulating pipes are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to the collecting plates.
(2) According to the medical human body red blood cell culture method, the heat preservation mechanism is arranged at the bottom of the storage culture box, so that the storage culture box can be in a constant temperature state in the observation process, the red blood cell liquid cannot be supercooled or overheated, the activity of the red blood cells is effectively guaranteed, and the success rate of experiments is improved.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of a preferred embodiment of a red blood cell culturing apparatus according to the present invention;
FIG. 2 is a schematic view of the dispersion treatment mechanism shown in FIG. 1;
FIG. 3 is a schematic view of the overall internal structure shown in FIG. 1;
FIG. 4 is a schematic structural view of the heat-retaining mechanism shown in FIG. 2;
fig. 5 is a schematic structural view of the water supply mechanism shown in fig. 4.
In the figure: the cell storage mechanism 1, the storage culture box 11, the first through pipe 12, the second through pipe 13, the third through pipe 14, the sealing mechanism 2, the sealing cover plate 21, the sealing plug 22, the support mechanism 3, the support box 31, the chute 311, the limit knob 32, the heat preservation mechanism 4, the heat preservation pipe 41, the connecting pipe 42, the water inlet connector 43, the water outlet connector 44, the water supply mechanism 45, the water tank 451, the electric heating rod 452, the circulating pump 453, the dispersion treatment mechanism 5, the first collection plate 51, the first feed port 511, the second collection plate 52, the second feed port 521, the third collection plate 53, the third feed port 531, the guide rail 6, the limit hole 61, the handle 7, the liquid separation control mechanism 8, the first control knob 81, the second control knob 82 and the third control knob 83.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1-5, the method for culturing human red blood cells for medical use according to the present invention comprises the following steps:
firstly, extracting and separating human red blood cells;
step two, placing the human body red blood cells prepared in the step one into an artificially prepared culture solution for culturing, wherein the composition and the content of the artificially prepared culture solution are the same as those of healthy human body blood without red blood cells;
step three, putting the culture solution added with the red blood cells in the step two into a culture instrument for culture;
the culture instrument comprises a cell storage mechanism 1, a sealing mechanism 2, a supporting mechanism 3, a heat preservation mechanism 4, a dispersion treatment mechanism 5 and a liquid separation control mechanism 8; the supporting mechanism 3 is arranged at the bottom of the cell storage mechanism 1; the dispersion treatment mechanism 5 is connected inside the supporting mechanism 3 in a sliding manner; the dispersion processing mechanism 5 is positioned at the bottom of the cell storage mechanism 1; the top of the cell storage mechanism 1 is connected with the sealing mechanism 2 in a matching way; the outer side wall of the supporting mechanism 3 is connected with the liquid separation control mechanism 8; the bottom surface of the cell storage mechanism 1 is provided with the heat preservation mechanism 4.
Specifically, as shown in fig. 1, the cell storage mechanism 1 includes a storage cassette 11, a first flow pipe 12, a second flow pipe 13, and a third flow pipe 14, and the first flow pipe 12, the second flow pipe 13, and the third flow pipe 14 are communicated with the bottom surface of the storage cassette 1; the first, second and third flow pipes 12, 13, 14 are arranged in a staggered and inclined manner, the distances between the first, second and third flow pipes 12, 13, 14 and the side edges of the storage and cultivation box 11 gradually increase, and the lengths of the first, second and third flow pipes 12, 13, 14 gradually decrease; the red blood cell sap can be discharged through the first flow pipe 12, the second flow pipe 13 and the third flow pipe 14, so that the red blood cell sap can be quickly circulated without manual transfer.
Specifically, as shown in fig. 1, the sealing mechanism 2 includes a sealing cover plate 21 and a sealing plug 22, the sealing plug 22 is connected to the top opening of the storage and cultivation box 11 in a matching manner, the sealing cover plate 21 is disposed on the top of the sealing plug 22, the sealing cover plate 21 is of a transparent structure, and the area of the sealing cover plate 21 is larger than the area of the top opening of the storage and cultivation box 11; can fill in storage culture box 11 with sealing plug 22 in, seal the protection to storage culture box 11, prevent that external environment from disturbing the growth of red blood cell to adopt transparent construction's sealed apron 21, the growth situation of the cell of operating personnel observation of can being convenient for.
Specifically, as shown in fig. 1, the supporting mechanism 3 includes a supporting box 31 and a limiting knob 32, the supporting box 31 is disposed at the bottom of the storage and culture box 11, a sliding groove 311 is formed in an inner wall of the supporting box 31, both side walls of the supporting box 31 are in threaded connection with the limiting knob 32, the supporting box 31 is of a cavity structure, a side section of the supporting box 31 is U-shaped, and at least three sliding grooves 311 are formed in an inner wall of the supporting box 31 from top to bottom; the storage culture box 11 can be stably supported by the support mechanism 3, thereby ensuring the stability of the cell storage mechanism 1 and the dispersion processing mechanism 5.
Specifically, as shown in fig. 1, fig. 3, fig. 4 and fig. 5, the heat preservation mechanism 4 includes a heat preservation pipe 41, a connection pipe 42, a water inlet joint 43, a water outlet joint 44 and a water supply mechanism 45, the heat preservation pipe 41 is installed on the bottom surface of the storage culture box 11, the interiors of the heat preservation pipes 41 are communicated with each other through the connection pipe 42, one end of the heat preservation pipe 41 is connected with the water inlet joint 43, the other end of the heat preservation pipe 41 is connected with the water outlet joint 44, the water inlet joint 43 and the water outlet joint 44 are both communicated with the water supply mechanism 45 through a conduit, the heat preservation pipes 41 are distributed in a horizontal array, and the length of the heat preservation pipe 41 is the same as the width of the storage culture box 11; the water supply mechanism 45 can supply water into the thermal insulation tube 41, and the temperature of the culture medium in the storage cassette 11 can be changed by the thermal insulation tube 41, so that the cells are cultured and observed at an appropriate temperature.
Specifically, as shown in fig. 4 and 5, the water supply mechanism 45 includes a water tank 451, an electric heating rod 452, and a circulation pump 453, one end of the circulation pump 453 is connected to the water inlet joint 43 through a pipe, the other end of the circulation pump 453 is connected to the inside of the water tank 451 through a pipe, and the electric heating rod 452 is installed inside the water tank 451; the electric heating rod 452 can heat water to realize hot water processing.
Specifically, as shown in fig. 2 and 3, the dispersion treatment mechanism 5 includes a first collecting plate 51, a second collecting plate 52, and a third collecting plate 53, the first collecting plate 51, the second collecting plate 52 and the third collecting plate 53 are all cooperatively connected with the sliding slot 311, a first feed inlet 511 is arranged on the surface of the first collecting plate 51 near the side edge, the first feed inlet 511 is arranged opposite to the bottom end of the first flow pipe 12, a second feed opening 521 is arranged on the surface of the second collecting plate 52 near the side edge, the second feed opening 521 is arranged opposite to the bottom end of the second flow pipe 13, a third feeding port 531 is arranged on the surface of the third collecting plate 53 near the side edge, the third feeding port 531 is arranged opposite to the bottom end of the third through pipe 14, the lengths of the first collecting plate 51, the second collecting plate 52 and the third collecting plate 53 are gradually decreased; in order to match with different flow tubes, the red blood cell liquid is collected in a dispersing way, so that an operator can observe and detect conveniently.
Specifically, as shown in fig. 2, guide rails 6 are symmetrically installed on the side walls of the first collecting plate 51, the second collecting plate 52 and the third collecting plate 53, handles 7 are respectively installed on the outer walls of the first collecting plate 51, the second collecting plate 52 and the third collecting plate 53, the guide rails 6 are connected with the sliding grooves 311 in a matched manner, a limiting hole 61 is formed at the end of the guide rails 6, and the limiting hole 61 is in threaded connection with the limiting knob 32; the operator can insert or pull out the collecting plate by holding the handle 7, so that the collecting plate is more convenient to operate.
Specifically, as shown in fig. 3, the dispensing control mechanism 8 includes a first control knob 81, a second control knob 82, and a third control knob 83, the first control knob 81 is connected to the bottom end of the first flow pipe 12 by a screw, the second control knob 82 is connected to the bottom end of the second flow pipe 13 by a screw, and the third control knob 83 is connected to the bottom end of the third flow pipe 14 by a screw; the flow process of the red blood cell fluid is controlled, and the operation is flexible and simple.
The dispersion processing mechanism 5 and the liquid separation control mechanism 8 can enable an operator to disperse the red blood cell liquid from the cell culture box to each collecting plate according to the experiment requirement, so that the preparation of a plurality of culture carriers is realized, manual transfer preparation is not needed, the operator can detect the states of the red blood cell liquid in different collecting plates, and more accurate experiment data can be obtained; and each collecting plate and the circulating pipe are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to each collecting plate; the heat preservation mechanism 4 is arranged at the bottom of the storage culture box 11, so that the storage culture box 11 can be in a constant temperature state in the observation process, the red blood cell liquid can not be supercooled or overheated, the activity of the red blood cells is effectively ensured, and the success rate of the experiment is improved; the method specifically comprises the following steps:
(1) placing the red blood cells to be cultured in the storage culture box 11, then covering a sealing cover plate 21, and carrying out closed culture on the storage culture box 11; the first collecting plate 51 can be inserted into the chute 311 at the bottom layer, then the limiting knob 32 at the corresponding position is screwed, the operations are repeated, the second collecting plate 52 and the third collecting plate 53 are inserted into the chute 311, the limiting knob 32 is screwed up, and at the moment, the feed inlet of each collecting plate is tightly communicated with the runner pipe at the corresponding position;
(2) when the contrast observation operation is needed, the first control knob 81 can be unscrewed, the first through pipe 12 is opened, the red blood cell liquid enters the first collecting plate 51 through the first through pipe 12 and the first feed port 511 for collection, when the required amount is collected, the first control knob 81 can be screwed down again, the limit knob 32 can be unscrewed, and the first collecting plate 51 can be drawn out through the handle 7; then, according to the experiment requirement, the second control knob 82 and the third control knob 83 are opened, so that the red blood cell fluid enters the second collecting plate 52 and the third collecting plate 53, and the states of the red blood cell fluid between the collecting plates are the same or different;
(3) in the process of culturing, when needing to observe the red blood cell liquid, can take out the culture apparatus, then with water tank 451 and articulate, electric rod 452 heats the water in water tank 451 to suitable temperature, then starts circulating pump 453 for warm water flows through in connecting pipe 42, the insulating tube 41, thereby makes the red blood cell be under optimum temperature all the time, avoids cell death.
According to the invention, through the dispersion treatment mechanism 5 and the liquid separation control mechanism 8, an operator can disperse the red blood cell liquid from the cell culture box to each collection plate according to experiment requirements, so that the preparation of a plurality of culture carriers is realized, and the operator can detect the states of the red blood cell liquid in different collection plates, thereby obtaining more accurate experiment data; and each collecting plate and the circulating pipe are arranged independently, so that the red blood cell liquid does not interfere with each other in the process of circulating to each collecting plate; install heat preservation mechanism 4 in the bottom of storing culture box 11, can be so that store culture box 11 and be in the constant temperature state in the observation process, red blood cell liquid can not supercool or overheated, the effectual activity of guaranteeing red blood cell has improved the success rate of experiment.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A medical human body red blood cell culture method is characterized by comprising the following steps:
firstly, extracting and separating human red blood cells;
step two, placing the human body red blood cells prepared in the step one into an artificially prepared culture solution for culturing, wherein the composition and the content of the artificially prepared culture solution are the same as those of healthy human body blood without red blood cells;
step three, putting the culture solution added with the red blood cells in the step two into a culture instrument for culture;
the culture instrument comprises a cell storage mechanism (1), a sealing mechanism (2), a supporting mechanism (3), a heat preservation mechanism (4), a dispersion treatment mechanism (5) and a liquid separation control mechanism (8); the supporting mechanism (3) is arranged at the bottom of the cell storage mechanism (1); the dispersion treatment mechanism (5) is connected to the inside of the supporting mechanism (3) in a sliding manner; the dispersion treatment mechanism (5) is positioned at the bottom of the cell storage mechanism (1); the top of the cell storage mechanism (1) is connected with the sealing mechanism (2) in a matching way; the outer side wall of the supporting mechanism (3) is connected with the liquid separation control mechanism (8); the heat preservation mechanism (4) is arranged on the bottom surface of the cell storage mechanism (1);
the cell storage mechanism (1) comprises a storage culture box (11), a first flow pipe (12), a second flow pipe (13) and a third flow pipe (14), wherein the first flow pipe (12), the second flow pipe (13) and the third flow pipe (14) are communicated with the bottom surface of the storage culture box (1); the first flow pipe (12), the second flow pipe (13) and the third flow pipe (14) are distributed in a staggered and inclined mode, the distance between the first flow pipe (12), the second flow pipe (13) and the third flow pipe (14) and the side edge of the storage culture box (11) is gradually increased, and the lengths of the first flow pipe (12), the second flow pipe (13) and the third flow pipe (14) are gradually reduced.
2. The method for culturing human red blood cells according to claim 1, wherein the method comprises the following steps: sealing mechanism (2) are including sealed apron (21) and sealing plug (22), sealing plug (22) with the top opening part of storing and cultivateing box (11) is mutually supported and is connected, the top of sealing plug (22) is equipped with sealed apron (21), sealed apron (21) are transparent structure, just the area of sealed apron (21) is greater than the top opening area of storing and cultivateing box (11).
3. The method for culturing human red blood cells according to claim 1, wherein the method comprises the following steps: supporting mechanism (3) are including supporting box (31) and limit knob (32), support box (31) and locate store the bottom of cultivateing box (11), the inner wall of supporting box (31) is opened there is spout (311), the equal threaded connection of both sides wall of supporting box (31) has limit knob (32), it is the cavity structure to support box (31), the side section that supports box (31) is the U style of calligraphy, the inner wall that supports box (31) is opened from top to bottom has at least three spout (311).
4. The method for culturing human red blood cells according to claim 1, wherein the method comprises the following steps: heat preservation mechanism (4) include insulating tube (41), connecting pipe (42), water supply connector (43), water connectors (44) and water supply mechanism (45), insulating tube (41) install in store the bottom surface of cultivateing box (11), pass through between insulating tube (41) inside connecting pipe (42) communicate each other, the one end of insulating tube (41) is connected water connectors (43), the other end of insulating tube (41) is connected water connectors (44), water connectors (43), water connectors (44) all communicate to through the pipe water supply mechanism (45), insulating tube (41) are horizontal array form and distribute, just the length of insulating tube (41) with the width of storing and cultivateing box (11) is the same.
5. The method for culturing human red blood cells according to claim 4, wherein the method comprises the following steps: the water supply mechanism (45) comprises a water tank (451), an electric heating rod (452) and a circulating pump (453), one end of the circulating pump (453) is connected with the water inlet joint (43) through a conduit, the other end of the circulating pump (453) is communicated to the inside of the water tank (451) through a conduit, and the electric heating rod (452) is installed inside the water tank (451).
6. The method for culturing human red blood cells according to claim 3, wherein the method comprises the following steps: the dispersion treatment mechanism (5) comprises a first collecting plate (51), a second collecting plate (52) and a third collecting plate (53), the first collecting plate (51), the second collecting plate (52) and the third collecting plate (53) are matched and connected with the sliding groove (311), a first feeding hole (511) is formed in the position, close to the side edge, of the surface of the first collecting plate (51), the first feeding hole (511) is arranged opposite to the bottom end of the first flow pipe (12), a second feeding hole (521) is formed in the position, close to the side edge, of the surface of the second collecting plate (52), the second feeding hole (521) is arranged opposite to the bottom end of the second flow pipe (13), a third feeding hole (531) is formed in the position, close to the side edge, of the surface of the third collecting plate (53), the third feeding hole (531) is arranged opposite to the bottom end of the third flow pipe (14), the lengths of the first collecting plate (51), the second collecting plate (52), and the third collecting plate (53) are gradually reduced.
7. The method for culturing human red blood cells according to claim 6, wherein the method comprises the following steps: guide rail (6) are installed to the equal symmetry of lateral wall of first collecting plate (51), second collecting plate (52) and third collecting plate (53), first collecting plate (51), second collecting plate (52) and the outer wall of third collecting plate (53) all is equipped with handle (7), guide rail (6) with spout (311) are mutually supported and are connected, spacing hole (61) have been opened to the tip of guide rail (6), spacing hole (61) with threaded connection between spacing knob (32).
8. The method for culturing human red blood cells according to claim 1, wherein the method comprises the following steps: divide liquid control mechanism (8) including first control knob (81), second control knob (82) and third control knob (83), first control knob (81) with threaded connection between the bottom of first circulation pipe (12), second control knob (82) with threaded connection between the bottom of second circulation pipe (13), third control knob (83) with threaded connection between the bottom of third circulation pipe (14).
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