CN211645181U - Culture medium storage box and automatic liquid changing device - Google Patents
Culture medium storage box and automatic liquid changing device Download PDFInfo
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- CN211645181U CN211645181U CN201922231191.3U CN201922231191U CN211645181U CN 211645181 U CN211645181 U CN 211645181U CN 201922231191 U CN201922231191 U CN 201922231191U CN 211645181 U CN211645181 U CN 211645181U
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Abstract
The utility model relates to the technical field of medical apparatus, a culture medium preservation box and automatic liquid changing device are provided, wherein culture medium preservation box includes: the box body and the box cover are arranged on the box body in a covering mode, an accommodating cavity for accommodating a culture medium liquid bag is formed between the box body and the box cover, the box cover comprises a first cover body and a second cover body, the first cover body is provided with a first liquid outlet groove, the second cover body is provided with a second liquid outlet groove, the first liquid outlet groove and the second liquid outlet groove are spliced to form a liquid outlet hole when the box cover is covered on the box body, and the liquid outlet hole is used for enabling a pipeline of the culture medium liquid bag to extend out; the utility model provides an automatic trade liquid device has following advantage: through dividing into first lid and second lid with the lid to set up first drain tank and second drain tank, make after the box body is put into to the state of connecting the pipeline at the culture medium liquid bag, can also fit first lid and second lid on the box body, this design can avoid culture medium liquid bag just carry out the connecting tube after putting into the box body, and then avoids the risk of dying fungus.
Description
Technical Field
The utility model relates to the technical field of medical appliances, more specifically say, relate to a culture medium preservation box and automatic liquid device that trades.
Background
Peripheral nerve injury repair is a common concern in multiple disciplines and research fields, such as tissue engineering, rehabilitation engineering, neurosurgery, and the like. At present, tissue engineering scaffolds, blood vessels or other nerve bundles are implanted into defective nerves to perform nerve repair mainly by methods such as tissue engineering, autografting and the like. However, autografting is limited by donors, and most tissue engineering scaffolds create an environment suitable for nerve regeneration, and defects which can be repaired by means of damaged nerve regeneration are limited. Therefore, in vitro culture and acquisition of nerve bundles by electrical stimulation, mechanical stimulation, chemical induction and the like become hot spots traced by everyone. At present, there are a lot of documents reporting from multiple levels of results, mechanisms, technical methods, etc., that electrical stimulation, mechanical stimulation and chemical intervention have effects on promoting nerve regeneration and neurite growth in vitro.
The method of engineering, chemistry and the like is adopted to intervene the growth and differentiation of nerve cells, bone cells and skin cells, and is the development direction of a plurality of fields such as the field of tissue engineering, the field of nerve engineering and the like. The utility model mainly aims at the long-term growth of mechanical intervention nerve tissues, and can also be expanded to be used for the long-term growth and differentiation of engineering means such as mechanics, electricity, chemistry and the like to intervene other kinds of cells.
The prior art has the following disadvantages: the culture medium preservation box in the automatic liquid changing device is complex to operate and risks bacterial contamination when the culture medium liquid bag is placed.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a culture medium preservation box to solve the big and complex operation's of operation process contamination risk technical problem who exists among the prior art.
In order to achieve the above object, the utility model discloses a technical scheme is a culture medium preservation box, include: box body and lid are located lid on the box body, the box body with form the chamber of acceping that is used for acceping the culture medium liquid bag between the lid, the lid includes first lid and second lid, first drain tank has been seted up to first lid, the second drain tank has been seted up to the second lid, first drain tank with the second drain tank in the lid in the time-lapse amalgamation forms out the liquid hole on the box body, it is used for the confession to go out the liquid hole the pipeline of culture medium liquid bag stretches out.
In one embodiment, the surface of the first cover body abutting against the second cover body is provided with an abutting groove, and the surface of the second cover body abutting against the first cover body is provided with an abutting column which is inserted into the abutting groove when the first cover body and the second cover body are covered on the box body.
In one embodiment, a first engaging portion is disposed on a side of the first cover close to the box, a second engaging portion is disposed on a side of the second cover close to the box, and the first engaging portion and the second engaging portion are engaged with the box when the first cover and the second cover are covered on the box.
In one embodiment, the culture medium storage box further comprises a conduction box embedded inside the culture medium storage box, the conduction box is provided with a refrigeration opening, and a refrigeration block is embedded in the refrigeration opening.
In one embodiment, the conduction box is provided with a conduction piece at the refrigeration opening, and the conduction piece is attached to the refrigeration piece of the refrigeration block.
In one embodiment, the conductive sheet is shaped and sized to fit the shape and size of the refrigeration sheet.
In one embodiment, the conductive box includes a first bending plate and a second bending plate mounted on the first bending plate, the first bending plate being assembled to the second bending plate.
In one embodiment, the culture medium storage box further comprises a liquid rack which is arranged in the conduction box and used for fixing the culture medium liquid bag, the cross section of the liquid rack is shaped like a Chinese character 'jing', and the culture medium liquid bag is arranged in the middle of the liquid rack.
In one embodiment, the liquid rack is provided with drain holes.
Another object of the utility model is to provide an automatic liquid changing device, including as above the culture medium storage box.
The utility model provides a culture medium preservation box and automatic liquid device that trades's beneficial effect lies in:
when putting into culture medium save box, need set up a pipeline and stretch out outside culture medium save box, the utility model discloses a divide into two parts with the lid, first lid and second lid to set up first drain tank on first lid, the second drain tank that sets up on the second lid, make after the box body is put into to the state of connecting the pipeline at culture medium liquid bag, can also fit first lid and second lid on the box body, the liquid outlet hole that the pipeline was formed by the cooperation of first drain tank and second drain tank stretches out, this design can avoid culture medium liquid bag just to carry out the connecting tube after putting into the box body, and then avoids the risk of dying fungus.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a perspective view of a medium storage case according to an embodiment of the present invention;
FIG. 2 is an exploded view of a media storage case according to an embodiment of the present invention;
FIG. 3 is an exploded view of another perspective of a media storage cassette according to an embodiment of the present invention.
The figures are numbered:
a box body 1;
a box cover 2; a first cover 21; a second cover 22; a first effluent tank 23; a second effluent trough 24; a docking slot 25; a docking post 26; a first fitting portion 27; a second fitting portion 28;
a culture medium bag 3; a pipe 31;
a conductive box 4; a refrigeration opening 41; a conductive sheet 42; a first bending plate 43; a second bending plate 44;
a refrigerating block 5; a refrigerating sheet 51;
a liquid rack 6; and a drain hole 61.
Detailed Description
In order to make the technical problem, technical solution and advantageous effects to be solved by the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected or indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the invention, and are not intended to indicate that a device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.
Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating relative importance or as indicating a number of technical features. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise. The following describes the specific implementation of the present invention in more detail with reference to specific embodiments:
as shown in fig. 1 to fig. 3, an embodiment of the present invention provides a culture medium storage box, including: a box body 1 and a box cover 2; this lid 2 lid is located on box body 1, forms the chamber of acceping that is used for acceping culture medium liquid bag 3 between box body 1 and the lid 2, and lid 2 includes first lid 21 and second lid 22, and first drain tank 23 has been seted up to first lid 21, and second drain tank 24 has been seted up to second lid 22, and first drain tank 23 and second drain tank 24 amalgamation form the liquid hole when lid 2 lid is on box body 1, and the liquid hole is used for supplying the pipeline 31 of culture medium liquid bag 3 to stretch out.
The working principle of the medium storage case of this embodiment is as follows:
the culture medium storage box is used for storing a culture medium, the culture medium is used for promoting the growth of nerves, the culture medium is stored in a culture medium liquid bag 3, the culture medium liquid bag 3 is communicated with the pipeline 31 before being placed in the box body 1, the culture medium liquid bag 3 communicated with the pipeline 31 is placed in the box body 1, the first cover body 21 and the second cover body 22 are respectively covered on the box body 1, the first liquid outlet groove 23 and the second liquid outlet groove 24 are opposite to the pipeline 31, and the pipeline 31 is arranged in a liquid outlet hole formed by splicing the first liquid outlet groove 23 and the second liquid outlet groove 24 in a penetrating manner; the tube 31 is connected to a pump assembly which is actuated to draw the culture medium from the culture medium fluid bag 3 for use.
The beneficial effect of the culture medium preservation box that this embodiment provided lies in:
culture medium liquid bag 3 when putting into culture medium save box, need set up a pipeline 31 and stretch out outside culture medium save box, the utility model discloses a divide into two parts with lid 2, first lid 21 and second lid 22, and set up first drain tank 23 on first lid 21, second drain tank 24 that sets up on second lid 22, make and put into box body 1 back under the state of connecting pipeline 31 at culture medium liquid bag 3, can also fit first lid 21 and second lid 22 lid on box body 1, pipeline 31 is stretched out by the play liquid hole that first drain tank 23 and second drain tank 24 cooperation formed, this design can avoid culture medium liquid bag 3 just to carry out connecting tube 31 after putting into box body 1, and then avoid the risk of dying fungus.
In one embodiment, the surface of the first cover 21 that is abutted against the second cover 22 is provided with an abutting groove 25, the surface of the second cover 22 that is abutted against the first cover 21 is provided with an abutting post 26, and the abutting post 26 is inserted into the abutting groove 25 when the first cover 21 and the second cover 22 are covered on the box body 1. Specifically, the docking post 26 is in clearance fit with the docking slot 25, and the mating between the docking post 26 and the docking slot 25 plays a guiding role, so as to align the first liquid outlet slot 23 and the second liquid outlet slot 24 to form a liquid outlet hole, and in addition, the mating between the docking post 26 and the docking slot 25 can also make the first cover 21 and the second cover 22 not easily move relative to each other after covering the box body 1, thereby avoiding that the liquid outlet hole cannot be formed due to the misalignment between the first cover 21 and the second cover 22.
In one embodiment, a first engaging portion 27 is disposed on a side of the first cover 21 close to the box 1, a second engaging portion 28 is disposed on a side of the second cover 22 close to the box 1, and the first engaging portion 27 and the second engaging portion 28 are engaged with the box 1 when the first cover 21 and the second cover 22 are covered on the box 1. Specifically, the first fitting portion 27 and the second fitting portion 28 are U-shaped and have a shape that is interference-fitted to the opening of the case 1, and it can be understood that the first cover 21 and the second cover 22 are easily fitted to the case 1 by providing the first fitting portion 27 and the second fitting portion 28.
In one embodiment, the culture storage box further comprises a conduction box 4 embedded inside the culture storage box, the conduction box 4 is provided with a refrigeration opening 41, and the refrigeration opening 41 is embedded with a refrigeration block 5. As can be understood, the temperature of the refrigeration block 5 can be uniformly conducted into the culture medium storage box by arranging the conduction box 4, so that the quality of the storage environment of the culture medium storage box is improved.
In one embodiment, the conductive box 4 is provided with a conductive sheet 42 at the refrigeration opening 41, the conductive sheet 42 adhering to the refrigeration sheet 51 of the refrigeration block 5. It can be understood that, the conductive sheet 42 is attached to the refrigeration sheet 51, so that the temperature of the refrigeration sheet 51 can be timely transmitted to the conductive sheet 42 with less loss, and then transmitted to the conductive box 4 through the conductive sheet 42, and thus, the refrigeration efficiency can be improved.
In one embodiment, the conductive sheet 42 is shaped and sized to fit the shape and size of the refrigeration sheet 51. In particular, the shape and size of the conductive sheet 42 are the same as those of the refrigeration sheet 51, and it can be understood that the temperature of the refrigeration sheet 51 can be more efficiently transferred to the conductive sheet 42 by this design.
In one embodiment, the conductive box 4 is a copper box comprising a first bending plate 43 and a second bending plate 44, the second bending plate 44 being assembled on the first bending plate 43. It can be understood that the copper box has good heat conductivity, the temperature of the refrigeration sheet 51 can be more efficiently conducted to the inside of the culture medium storage box through the copper box, and the copper box is assembled by the first bending plate 43 and the second bending plate 44, and has a simple structure.
In one embodiment, the culture medium storage box further comprises a liquid shelf 6 arranged in the conduction box 4 and used for fixing the culture medium liquid bag 3, the cross section of the liquid shelf 6 is shaped like a Chinese character 'jing', and the culture medium liquid bag 3 is arranged in the middle of the liquid shelf 6. In this embodiment, the culture medium liquid bag 3 is placed in the middle of the liquid shelf 6, and the liquid shelf 6 separates the culture medium liquid bag 3 from the box wall of the conduction box 4 by a preset distance, so as to prevent the culture medium liquid bag 3 from contacting the copper box and ensure that the storage temperature of each part of the culture medium in the culture medium liquid bag 3 is consistent. The user can also place the dampproof agent between liquid frame 6 and conduction box 4, adsorbs steam, guarantees good save environment.
In one embodiment, the liquid rack 6 is provided with a drain hole 61. It can be understood that the arrangement of the circular holes on the liquid rack 6 does not block the cold air while preventing the condensed water from accumulating on the liquid rack 6.
In one embodiment, the box body 1 and the box cover 2 are made of plastic foam or other heat insulating materials.
Another object of the utility model is to provide an automatic liquid changing device, including as above the culture medium storage box.
The automatic liquid changing device provided with the culture medium preservation box has the advantages of being simple to operate and small in contamination risk.
The automatic liquid changing device is used for changing a culture medium for the environment for growing nerves in the process of extracting long-section nerves.
In the existing nerve treatment technology, "autografting" is regarded as the "gold standard" of clinical treatment of peripheral nerve injury. However, "autografting" is often limited to donor sources, and most currently involve the use of nerve bundles in other, non-fit critical functional areas of the patient as donors, and sacrifice of other nerve functions in the patient. Another approach is to use body tubular tissue such as blood vessels, trachea, etc. for "bridging" to create an environment suitable for nerve growth, but is limited to the donor as in the above approach. With the development of tissue engineering technology, various types of tissue engineering scaffolds are created, and the surface of the tissue engineering scaffold is modified by designing a proper tissue engineering scaffold to induce nerve regeneration. Some institutional workers have had more attempts and efforts in this regard to using chitosan as a scaffolding material and have demonstrated that this approach does not treat defects exceeding 30 mm. In view of the above problems, a method for obtaining long nerve segments in vitro has been proposed: the axon bundle emitted from the dorsal root ganglion is pulled in one direction by external force to obtain long-section active nerve bundles, and then the long-section active nerve bundles are grafted and transplanted by combining a tissue engineering method. Through experimental verification, nerve can be elongated through the mode that the tractive was cultivateed, but owing to need cultivate for a long time in the experimentation, through a period of cultivation back, because the accumulation of the consumption of nutrition and metabolic waste needs to be changed the culture medium, and manual liquid changing is not only loaded down with trivial details and has the fungus risk of dying, the utility model discloses this automatic liquid changing device has mainly been designed to this kind of condition.
The utility model discloses mainly be applied to and acquire long-range nerve bundle, but also can be used to the long-term culture of other biological tissue simultaneously.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A media storage cassette, comprising: box body and lid are located lid on the box body, the box body with form the chamber of acceping that is used for acceping the culture medium liquid bag between the lid, the lid includes first lid and second lid, first drain tank has been seted up to first lid, the second drain tank has been seted up to the second lid, first drain tank with the second drain tank in the lid in the time-lapse amalgamation forms out the liquid hole on the box body, it is used for the confession to go out the liquid hole the pipeline of culture medium liquid bag stretches out.
2. The media storage cassette of claim 1, wherein a surface of the first cover that interfaces with the second cover is provided with an interface slot, and a surface of the second cover that interfaces with the first cover is provided with an interface post that is inserted into the interface slot when the first cover and the second cover are placed on the cassette body.
3. The medium storing cassette according to claim 1, wherein a first engaging portion is provided on a side of the first lid member adjacent to the cassette body, a second engaging portion is provided on a side of the second lid member adjacent to the cassette body, and the first engaging portion and the second engaging portion are engaged with the cassette body when the first lid member and the second lid member are attached to the cassette body.
4. The culture medium storage box of claim 1, further comprising a conduction box embedded inside the culture medium storage box, wherein the conduction box is provided with a refrigeration opening, and a refrigeration block is embedded in the refrigeration opening.
5. The media storage cassette of claim 4, wherein the conduction cassette is provided with a conduction tab at the refrigeration opening, the conduction tab engaging a refrigeration tab of the refrigeration block.
6. The media retention cassette of claim 5, wherein the conductive sheet is shaped and sized to fit the shape and size of the refrigeration sheet.
7. The media storage cassette of claim 4, wherein the conductive cassette comprises a first folding plate and a second folding plate mounted on the first folding plate, the first folding plate being assembled to the second folding plate.
8. The media storage cassette of claim 4, further comprising a liquid shelf disposed within the conduction cassette for holding the media bag, the liquid shelf having a cross-sectional shape of a "well" shape, the media bag being disposed in a middle portion of the liquid shelf.
9. The medium-storing cassette according to claim 8, wherein a drain hole is provided in the liquid rack.
10. An automatic fluid changing device comprising the medium storing cassette according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922231191.3U CN211645181U (en) | 2019-12-12 | 2019-12-12 | Culture medium storage box and automatic liquid changing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922231191.3U CN211645181U (en) | 2019-12-12 | 2019-12-12 | Culture medium storage box and automatic liquid changing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211645181U true CN211645181U (en) | 2020-10-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201922231191.3U Active CN211645181U (en) | 2019-12-12 | 2019-12-12 | Culture medium storage box and automatic liquid changing device |
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| CN (1) | CN211645181U (en) |
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- 2019-12-12 CN CN201922231191.3U patent/CN211645181U/en active Active
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