CN108403872B

CN108403872B - Herbal essence capable of dispelling wind, removing dampness and relieving pain

Info

Publication number: CN108403872B
Application number: CN201810425820.3A
Authority: CN
Inventors: 黄金英; 宇文军利; 黄文宇
Original assignee: Shenzhen Runbang Zhijia Biotechnology Co ltd
Current assignee: Shenzhen Runbang Zhijia Biotechnology Co ltd
Priority date: 2018-05-07
Filing date: 2018-05-07
Publication date: 2021-05-18
Anticipated expiration: 2038-05-07
Also published as: CN108403872A

Abstract

The invention discloses a wind-dispelling dampness-removing pain-relieving herbal essence. The essence mainly comprises 1, 2-propylene glycol, 1, 3-butylene glycol, a cinnamon extract, a safflower extract, a crocus sativus extract, a garden balsam stem extract, a cinnamomum camphora bark extract and an angelica sinensis extract. The safflower extract, the crocus extract, the garden balsam stem extract and the cinnamomum camphora peel extract are prepared by enzyme extraction combined with ultrasonic-synergistic electrostatic field extraction. Compared with the prior art, the wind-dispelling, dampness-removing and pain-relieving herbal essence prepared by the invention has the advantages of elegant smell, high public acceptance, convenience in use, quickness in absorption and remarkable wind-dispelling, dampness-removing and pain-relieving effects.

Description

Herbal essence capable of dispelling wind, removing dampness and relieving pain

Technical Field

The invention relates to an essence, and particularly relates to a wind-dispelling, dampness-removing and pain-relieving herbal essence.

Background

In traditional Chinese medicine, wind-cold-dampness-heat belongs to the category of arthralgia syndrome in traditional Chinese medicine. The rheumatism is caused by the obstruction of meridian tissues caused by the invasion of meridian or endogenous phlegm dampness, heat toxin and blood stasis due to the deficiency of vital qi in vivo, wind cold and dampness heat and the like, and causes pain, numbness, swelling and inflexibility of limbs, joints and muscles. The traditional Chinese medicine composition is mainly used for treating the disease by promoting blood circulation to remove blood stasis, dispelling wind and dredging collaterals, removing turbidity and opening coagulation, smoothing qi and blood, treating both symptoms and root causes and eliminating pain. At present, a plurality of western medicines for treating the disease can not be cured radically, and have large side effect, some patients take analgesics for a long time for relieving pain and a large amount of hormone medicines for relieving the disease, so that the pain can be relieved, the activity is improved, and the disease condition is relieved. Symptoms may reappear and worsen once the medication is discontinued. The repeated attack may cause yellow and edema of face, osteoporosis and osteonecrosis, resulting in aggravation of the disease. So far, because the internal and external diseases still belong to intractable diseases with unknown causes, some patients take prednisone and dexamethasone as analgesics, so that the pain is slight, the pain is less, the pain is more, the analgesics cannot be removed all the year round, the patients can live for months, the joint swelling, the rigidity and the deformation are caused, the mobility is lost, and great pain is brought to families of the patients.

At present, China has a large number of patients with rheumatism and discomfort of bones, joints and muscles, and the patients can not take care of the life seriously. In the face of patients of the type, a green practical product which can relieve and treat the diseases and has no side effect is urgently needed to meet the requirements of the patients of the type, and no external medicine which can effectively treat rheumatism and bone and joint discomfort exists at present.

The invention provides a wind-dispelling dampness-removing pain-relieving herbal essence which is developed specially for pain symptoms, and the essence is free of hormone, stimulation, heating agent, analgesic, preservative and chemical additive, is convenient to use and is completely efficient. Is suitable for patients with rheumatic osteodynia and strain pain, and sub-health people with soreness, numbness and distending pain such as lower head group, computer group, mahjong group, and driver group. The medicine is in contact with the painful part in a ball rolling way, and quickly permeates to the mass part of the muscles and bones in 3-5 minutes by virtue of the acupuncture principle, so as to achieve the effects of expelling cold and dampness, softening tendons and knots, and relieving pain and stopping pain.

Disclosure of Invention

In order to achieve the purpose, the invention provides a wind-dispelling dampness-removing pain-relieving herbal essence which comprises the following components: 1, 2-propylene glycol, 1, 3-butylene glycol, cinnamon extract, safflower extract, crocus extract, garden balsam stem extract, camphor bark extract and angelica extract.

Preferably, the wind-dispelling dampness-removing pain-relieving herbal essence consists of the following raw materials in percentage by weight: 2-5% of 1, 2-propylene glycol, 1-5% of 1, 3-butanediol, 13-20% of cinnamon extract, 10-20% of safflower extract, 15-20% of crocus sativus extract, 8-15% of garden balsam stem extract, 5-21% of cinnamomum camphora bark extract and 15-25% of angelica sinensis extract.

1, 2-propylene glycol: it is colorless viscous liquid in normal state, almost tasteless, and slightly sweet. Can be used as humectant or solvent in cosmetic, toothpaste and soap in combination with glycerol or sorbitol.

1, 3-butanediol: the butanediol moisturizing agent is one of polyhydric alcohols, is often used as a moisturizing agent and a solvent in cosmetics, has small water-holding proportion and also has a certain bacteriostatic action in the aspect of moisturizing because the butanediol is a small-molecular moisturizing component. The 1, 3-butanediol has good safety. The test showed that when the application was carried out intermittently on a human body, the application was carried out once every other day for 16 days, and no irritating inflammation was found in all 200 persons who participated in the test. The irritation to the eye mucosa has been tested in mice and the results obtained are still very safe.

Cinnamon: latin learning name: cinnamomum cassia Presl, also named as Cinnamomum cassia, Cinnamomum japonica, Cinnamomum cassia, Ping' an tree, and Chinese cinnamon bark, can be used for treating sexual impotence, cold womb, cold pain of waist and knee, asthma due to kidney deficiency, floating upward due to deficiency yang, giddiness, conjunctival congestion, cold pain of heart and abdomen, vomiting and diarrhea due to deficiency cold, cold hernia, abdominal pain, dysmenorrhea and amenorrhea.

Safflower: latin learning name: carthamus tinctorius L has the effects of promoting blood circulation, dredging channels, removing blood stasis and relieving pain, and is helpful for treating amenorrhea, dysmenorrhea, lochiorrhea, thoracic obstruction, cardiodynia, stasis and abdominal pain, stabbing pain in chest and hypochondrium, traumatic injury, sore and ulcer with swelling and pain. Safflower is rich in glycosides, polysaccharides and organic acids. Promoting blood circulation, dredging channels, and removing blood stasis. Has effects in lowering blood pressure, reducing blood lipid, and improving microcirculation.

Crocus sativus: latin learning name: crocus sativus L, Crocus sativus is a famous precious traditional Chinese medicine, and the main medicinal part is small stigmas. The saffron contains carotene compounds, including crocin, dimethyl croconate, picroside and volatile oil, and safranic aldehyde. Its dry stigma has sweet taste and mild nature, and can promote blood circulation, remove blood stasis, disperse stagnation, and relieve pain. Can be used for treating melancholia, chest distress, hematemesis, typhoid fever, menoxenia, puerperal lochiorrhea, blood stasis, measles, traumatic injury, etc. Is often used as sedative and expelling pathogenic wind agent. Promoting blood circulation, removing blood stasis, cooling blood, removing toxic substance, resolving stagnation, and tranquilizing mind. Warm poison, macula, melancholy, stuffiness, palpitation, and mania.

Garden balsam stem: latin learning name: pharyma leptostachya l.subsp.asitica (Hara) Kitamura, also known as aspergilli, muscardine etc. Has effects in expelling pathogenic wind, removing dampness, relaxing muscles and tendons, activating collateral flow, promoting blood circulation, dispelling blood stasis, promoting urination, removing toxic materials, and dredging channels and bones. The fresh grass can be pounded and externally applied for treating sore, furuncle, swelling and pain, and insect bite. The decoction has analgesic and antirheumatic effects.

Camphor wood: latin learning name: cinanmum camphora (L.) presl, Cinnamomum camphora, also known as camphorwood, is the mature fruit of Cinnamomum camphora and is mainly used for dispelling wind and cold; warming stomach and regulating stomach; regulating qi-flowing for relieving pain. Cold pain in the main epigastrium and abdomen; vomiting and diarrhea due to cold-dampness; abdominal distension due to qi stagnation; beriberi. The camphor tree bark is mainly used for promoting qi circulation, relieving pain and dispelling wind-damp. It is used to treat vomiting, diarrhea, stomachache, rheumatic arthralgia, beriberi, scabies, tinea, and traumatic injury. The bark of cinnamomum camphora contains levo-epicatechin (epicatechin), dextro-epicatechin (epicatechin), procyanidin (procyanidins) B1, B2, B7, C1, cinnamyl tannin I (cinnamontanin I), propionic acid (propionicacid), butyric acid (butyric acid), valeric acid (valericaceic acid), caproic acid (caproic acid), caprylic acid (caprylic acid), capric acid (capric acid), lauric acid (lauric acid), myristic acid (myristic acid), stearic acid (stearic acid), oleic acid (oleic acid), and myristic acid (myristoleicacid).

Chinese angelica: latin learning name: angelica sinensis, namely Angelica sinensis, ash, west Angelica, Min Angelica, golden Angelica, Angelica sinensis, cauda Angelica, Angelica koji, and Angelica sinensis; the root of it can be used as a medicine, and is one of the most commonly used Chinese herbs. Has effects in nourishing blood, regulating menstruation, relieving pain, moistening dryness, smoothing intestine, resisting cancer, resisting aging, and enhancing immunity.

Preferably, the cinnamon extract and the angelica extract are commercially available.

Preferably, the safflower extract, the saffron extract, the garden balsam stem extract and the camphor bark extract are prepared by the following preparation methods:

s1, drying the traditional Chinese medicine raw materials at 60-85 ℃ for 1-3 days, and crushing to 50-500 meshes to obtain traditional Chinese medicine powder; the traditional Chinese medicine raw materials are respectively safflower, crocus sativus, garden balsam stem and camphor bark;

s2, uniformly mixing the traditional Chinese medicine powder, water and the complex enzyme, carrying out enzymolysis for 1.5-2.5 h at the temperature of 40-60 ℃ and at the pH of 4.5, and filtering to obtain filtrate A and filter residue A;

preferably, the mass ratio of the traditional Chinese medicine powder to the water to the compound enzyme is 1 (6-12) to 0.1-0.3;

preferably, the complex enzyme is cellulase and/or pectinase;

further preferably, the complex enzyme is prepared by mixing cellulase and pectinase according to the mass ratio of 3: 1.

S3, adding the filter residue A and a stabilizer into an ethanol water solution with the mass fraction of 30-80%, extracting for 20-40 min at 40-60 ℃ by ultrasonic wave in cooperation with an electrostatic field, and filtering to obtain a filtrate B;

preferably, the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1 (0.02-0.06) to 10-15;

preferably, the stabilizer is one or more of ferrous sulfate, poloxamer 188 and chitosan;

preferably, the stabilizer is ferrous sulfate, poloxamer 188 and chitosan according to a mass ratio (1-3): (0.2-0.6): (1-3) mixing;

s4 mixing the filtrate A and B, evaporating under reduced pressure until no ethanol smell exists, and freezing and condensing to obtain Carthami flos extract or stigma croci Sativi extract or caulis et folium Gaultheriae Yunnanensis extract or cortex Cinnamomi Camphorae extract.

Preferably, the ultrasonic frequency is 20-40 KHz, the ultrasonic power is 100-300W, and the electrostatic voltage is 2.5-15 kV.

Preferably, the reduced pressure evaporation is carried out under the conditions of 40-60 ℃ and the absolute pressure of 0.085-0.095 MPa.

Preferably, the freezing and condensing is suspension freezing and condensing, the precooling temperature is 5-8 ℃, the stirring speed is 160-200 r/min, and the refrigerant temperature is-12 to-16 ℃.

A preparation method of a wind-dispelling dampness-removing pain-relieving herbal essence comprises the following steps: weighing raw materials according to a formula, and uniformly mixing 1, 2-propylene glycol, 1, 3-butylene glycol, a cinnamon extract, a safflower extract, a saffron extract, a garden balsam stem extract, a camphor bark extract and an angelica extract at 15-30 ℃ to obtain the wind-dispelling, dampness-removing and pain-relieving herbal essence.

Ultrasonic synergistic electrostatic field extraction: the ultrasonic wave can destroy the cell walls of the medicinal materials, so that effective substances are easily extracted from the medicinal materials; compared with the traditional method, the method has the advantages of high extraction rate, short extraction time, no need of high temperature in the whole extraction process, energy conservation, environmental protection and the like. The electrostatic field is introduced into the synergistic ultrasonic extraction, which is a new application field of power ultrasound, and the arrangement of water molecules is more ordered by the influence of an external direct current electric field on a water structure because the water molecules are strong polar molecules and the positive and negative electric centers of the water molecules are not overlapped and a molecular electric dipole moment exists. The electric field strengthening can generate small-sized oscillating liquid drops and increase the mass transfer ratio surface; promoting the internal circulation of small-size liquid drops and strengthening the mass transfer coefficient of dispersed phase liquid drops; furthermore, when the dispersed phase passes through the continuous phase, the interfacial shear stress is increased due to the electrostatic acceleration effect, thus enhancing the membrane mass transfer coefficient of the continuous phase.

Freezing and condensing: under normal pressure, the dilute solution and ice are in a solid-liquid phase equilibrium relationship below a freezing point, namely water molecules in the solution are solidified into ice crystals, and the ice is removed by a mechanical means, so that the solvent water in the solution is reduced, the concentration of the solution is improved, and the solution is concentrated. The freeze concentration is carried out at a low temperature below the freezing point of the solution without heating treatment, so that the thermal decomposition of components in the material and the volatilization of aromatic substances are avoided, and the original quality and flavor of the food are fully preserved. In addition, the whole processing operation is realized at low temperature, and the low temperature also inhibits the proliferation of microorganisms and reduces the pollution risk caused by the microorganisms. When a solution containing a plurality of solutes is concentrated by freezing, only water is removed, and other components are not changed.

The wind-dispelling dampness-removing pain-relieving herbal essence can be used by adopting the following method:

for headache, dizziness and carsickness: the traditional Chinese medicine composition is applied to Fengfu points, Taiyang points and cervical vertebra parts of the Fengchi, and is slightly rolled and massaged by balls for 5-10 minutes to absorb heat, so that pain can be relieved immediately after the traditional Chinese medicine composition is used up, and the traditional Chinese medicine composition can also be used in combination with head muscle stirring;

for shoulder and neck, lumbar and joint pain: most of the pain is associated with cold-dampness and damp-heat. When the essence is heated, cold and dampness and damp-heat can be discharged out of the body, and the specific components in the essence can feel slightly hot and pungent when being applied to an affected part, so that the essence is helpful for removing rheumatism, wind cold and damp-heat which cause pain in the body. Rolling and massaging the painful part for 5-10 minutes until the painful part is heated; can also be used together with folium Artemisiae Argyi to achieve the best effect;

bruise and bruise for sprain and fall injury: the ball is applied to the affected part and is absorbed until the affected part is heated, so that the effects of quickly activating blood circulation and removing blood stasis are achieved; it can also be used together with folium Artemisiae Argyi for better effect.

The wind-dispelling dampness-removing pain-relieving herbal essence provided by the invention has the following beneficial effects:

1. the traditional Chinese medicine has light traditional Chinese medicine taste, no sharp taste and high public acceptance.

2. The operation is convenient, and the pain can be applied by rolling, without manipulation, only by rolling, applying and absorbing.

3. The pain takes effect quickly! The pores and the acupuncture points are opened quickly to absorb the medicine property of the prescription, thereby achieving the effects of expelling wind, dispelling cold, removing dampness, promoting blood circulation to remove blood stasis, relieving swelling and pain, and softening tendons and resolving masses.

4. The utility model can be used by all people, and is convenient to operate, and can be used by the old and the young, and the old can use the plaster conveniently, where to roll and paint. Is convenient to carry, can not leave unpleasant smell on the body after use, and can be used conveniently at any time and any place on business trips.

5. The freeze condensation technology is adopted, the molecular weight is small, the product is super-concentrated, and the absorption is fast.

Detailed Description

Comparative example 1

The herbal essence capable of dispelling wind, removing dampness and relieving pain comprises the following raw materials in percentage by weight: 3% of 1, 2-propylene glycol, 3% of 1, 3-butylene glycol, 15% of cinnamon extract, 18% of safflower extract, 18% of crocus extract, 10% of garden balsam stem extract, 12% of cinnamomum camphora bark extract and 21% of angelica extract.

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s1 drying Carthami flos at 70 deg.C for 2 days, cutting, and pulverizing to 500 mesh with ZHY-450 Chinese medicinal superfine pulverizer (Jiangyin Jiangtuo mechanical equipment Co., Ltd.) to obtain Carthami flos powder;

s2 stirring and mixing the safflower medicinal powder, water and complex enzyme for 10min at 300 r/min to obtain a mixed solution, and adjusting the pH of the mixed solution to 4.5 by 0.1mol/L hydrochloric acid at a constant temperature of 50 ℃; carrying out enzymolysis on the mixed solution for 2h at the constant temperature of 50 ℃ at 300 r/min, measuring the pH of the mixed solution every 5 minutes during the enzymolysis, and adjusting the pH of the mixed solution to 4.5 by using 0.1mol/L sodium hydroxide or 0.1mol/L hydrochloric acid; filtering the mixed solution after the enzymolysis by using 100-mesh gauze, and adjusting the pH of the obtained filtrate to 7.0 by using 0.1mol/L sodium hydroxide aqueous solution at the constant temperature of 25 ℃ to obtain filtrate; the mass ratio of the safflower medicinal material powder to the water to the compound enzyme is 1:10: 0.2; the compound enzyme is formed by mixing cellulase and pectinase according to the mass ratio of 3: 1;

s3 evaporating the filtrate under reduced pressure until no ethanol smell is produced, and freezing and condensing to obtain Carthami flos extract; the temperature in the reduced pressure evaporation is 50 ℃, and the absolute pressure is 0.090 MPa; the freezing and condensing is suspended freezing and condensing; wherein the precooling temperature is 7 ℃, the stirring speed is 200r/min, and the refrigerant temperature is-15 ℃.

The preparation methods of the saffron extract, the garden balsam stem extract and the camphor bark extract are the same as the preparation method of the saffron extract.

A preparation method of a wind-dispelling dampness-removing pain-relieving herbal essence comprises the following steps: weighing the raw materials according to the formula, and stirring and mixing the 1, 2-propylene glycol, the 1, 3-butylene glycol, the cinnamon extract, the safflower extract, the saffron extract, the garden balsam stem extract, the camphor bark extract and the angelica extract at 300 r/min and 25 ℃ for 30min to obtain the wind-dispelling, dampness-removing and pain-relieving herbal essence.

Comparative example 2

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s2 adding Carthami flos powder into 50% ethanol water solution, extracting at 50 deg.C for 30min with ultrasonic and electrostatic field, and filtering with 100 mesh gauze to obtain filtrate; the mass ratio of the safflower medicinal material powder to the ethanol water solution is 1: 10; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

Comparative example 3

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s2 stirring and mixing the safflower medicinal powder, water and complex enzyme for 10min at 300 r/min to obtain a mixed solution, and adjusting the pH of the mixed solution to 4.5 by 0.1mol/L hydrochloric acid at a constant temperature of 50 ℃; carrying out enzymolysis on the mixed solution for 2h at the constant temperature of 50 ℃ at 300 r/min, measuring the pH of the mixed solution every 5 minutes during the enzymolysis, and adjusting the pH of the mixed solution to 4.5 by using 0.1mol/L sodium hydroxide or 0.1mol/L hydrochloric acid; filtering the mixed solution after the enzymolysis by using 100-mesh gauze, and adjusting the pH of the obtained filtrate to 7.0 by using 0.1mol/L sodium hydroxide aqueous solution at the constant temperature of 25 ℃ to obtain filtrate A; filtering to obtain filter residue a; the mass ratio of the safflower medicinal material powder to the water to the compound enzyme is 1:10: 0.2; the compound enzyme is formed by mixing cellulase and pectinase according to the mass ratio of 3: 1;

s3, adding the filter residue a into 50% ethanol water solution by mass fraction, extracting for 30min under the condition of constant temperature of 50 ℃ and ultrasonic synergy electrostatic field, and filtering by a 100-mesh gauze to obtain a filtrate B; the mass ratio of the filter residue a to the ethanol water solution is 1: 10; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

s4 stirring and mixing the filtrate A and the filtrate B for 10min at 300 r/m, evaporating under reduced pressure until no ethanol smell exists, and freezing and condensing to obtain Carthami flos extract; the temperature in the reduced pressure evaporation is 50 ℃, and the absolute pressure is 0.090 MPa; the freezing and condensing is suspended freezing and condensing; wherein the precooling temperature is 7 ℃, the stirring speed is 200r/min, and the refrigerant temperature is-15 ℃.

Example 1

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s3, adding the filter residue a and a stabilizer into an ethanol aqueous solution with the mass fraction of 50%, extracting for 30min under the condition of constant temperature of 50 ℃ by using ultrasonic waves in cooperation with an electrostatic field, and filtering by using a 100-mesh gauze to obtain a filtrate B; the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1:0.05: 10; the stabilizer is ferrous sulfate, poloxamer 188 and chitosan according to the mass ratio of 1:0.3: 1; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

Example 2

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s3, adding the filter residue a and a stabilizer into an ethanol aqueous solution with the mass fraction of 50%, extracting for 30min under the condition of constant temperature of 50 ℃ by using ultrasonic waves in cooperation with an electrostatic field, and filtering by using a 100-mesh gauze to obtain a filtrate B; the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1:0.05: 10; the stabilizer is ferrous sulfate and poloxamer 188 according to the mass ratio of 1: 0.3; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

Example 3

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s3, adding the filter residue a and a stabilizer into an ethanol aqueous solution with the mass fraction of 50%, extracting for 30min under the condition of constant temperature of 50 ℃ by using ultrasonic waves in cooperation with an electrostatic field, and filtering by using a 100-mesh gauze to obtain a filtrate B; the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1:0.05: 10; the stabilizer is poloxamer 188 and chitosan according to the mass ratio of 0.3: 1; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

Example 4

The cinnamon extract and the angelica extract are commercially available.

The safflower extract adopts the following preparation method:

s3, adding the filter residue a and a stabilizer into an ethanol aqueous solution with the mass fraction of 50%, extracting for 30min under the condition of constant temperature of 50 ℃ by using ultrasonic waves in cooperation with an electrostatic field, and filtering by using a 100-mesh gauze to obtain a filtrate B; the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1:0.05: 10; the stabilizer is ferrous sulfate chitosan according to the mass ratio of 1: 1; the ultrasonic synergistic electrostatic field conditions are ultrasonic frequency of 20KHz, ultrasonic power of 200W and electrostatic voltage of 10 kV;

Test example 1

In vitro transdermal permeability test:

the abdominal skin of Kunming mice was used as the penetrating skin. Taking 6 mice with similar body weight, cutting off the abdominal hair, and using Motet^TMAnd (3) thinly coating depilatory cream, cleaning, removing hair, feeding for 24 hours, respectively killing, taking skin, removing subcutaneous fat, and cleaning by using sterile normal saline to obtain the mouse skin.

Fixing the treated mouse skin between a sample pool and a receiving pool, and dripping the wind-dispelling, dampness-removing and pain-relieving herbal essence of comparative examples 1-3 and examples 1-4 on the cutin layer of the mouse skin (the dripping area is 2.5 cm)²2mL in drop coating amount) and tightly attached with a medical adhesive tape. Transdermal test was performed on a TK6-500 transdermal diffusion tester (Shanghai yellow sea medical testing Instrument laboratory). The tester is simultaneously provided with 6 diffusion cells, the cell structure is the same as that of a Franz cell, the size is reduced in proportion, and the area of the cell opening is about 2.5cm²The volume of each receiving pool is calibrated in advance, and the volume of each pool is 7.2-7.8 mL. The sample cell was fixed using 0.9% physiological saline as a receiving solution. Sampling at constant temperature (32 +/-0.5) DEG C for 2, 4, 6, 8, 10, 12 and 24 hours respectively, wherein the sampling amount is 0.5ml each time, supplementing an equal volume of fresh receiving solution, and determining the concentration of the carthamin, the crocin, the tuberculin C and the camphor in the receiving solution by an HPLC method, thereby comparing the cumulative transmittance of the herbal essence for dispelling wind, removing dampness and relieving pain. The test results are shown in Table 1.

TABLE 1 transdermal Permeability test

Test example 2

And (3) testing the physical therapy effect:

clinical physiotherapy is carried out on 700 sub-healthy people with shoulder and neck pain symptoms such as shoulder muscle strain, numbness of upper limbs, difficulty in flexion and extension of joints, pain and soreness, fatigue and dizziness by combining wind-dispelling, dampness-removing and pain-relieving herbal essence with massage, and simultaneously, the clinical physiotherapy is compared with 20 cases of a control group by combining vaseline with massage for physiotherapy. 20g each time, 30-60 minutes each time, 1 time per day, and 6 times in total as one treatment course, wherein 2 treatment courses (12 times) are continuously used; the results are shown in Table 2.

The treatment component comprises 7 groups of 100 cases, 50 cases of male and female respectively, the shortest disease period is 3 months, and the longest disease period is 15 years. The treatment groups 1 to 7 respectively adopt the wind-dispelling, dampness-removing and pain-relieving herbal essences prepared in the comparative examples 1 to 3 and the examples 1 to 4 to carry out physical therapy in combination with massage.

In 20 cases of the control group: 20 cases of men and women respectively have the shortest disease period of 3 months and the longest disease period of 15 years. The control group was treated with vaseline in combination with massage.

The criteria for determination are as follows:

high efficiency: the nodules on the shoulders disappear, the soreness and pain of the joints on the shoulders disappear, and the symptoms of fatigue, dizziness and numbness of the upper limbs disappear;

the effect is shown: the nodules on the shoulders basically disappear, the joint ache and pain basically disappear, and the symptoms of fatigue, dizziness and upper limb numbness basically disappear;

the method has the following advantages: the improvement on shoulder nodules, joint soreness, fatigue dizziness and upper limb numbness;

and (4) invalidation: the nodules on the shoulders are basically unchanged, the joint is sore and painful, the fatigue and the vertigo are caused, and the numbness feeling of the upper limbs is basically not eliminated.

TABLE 2 clinical trial of the test and control groups

Test example 3

Skin irritation test

6 guinea pigs (provided by the Experimental animals center of Chinese university of pharmacy) were weighed 250. + -.20 g and half female and half male. Removing hair from two sides of vertebral column of guinea pig 24 hr before administration, wherein the hair removing region is 8 × 5cm²And checked 24 hours after unhairing to ensure that the unhaired skin was not damaged.

The left and right sides of the same body are compared with each other, the hair-removing area on the left side is coated with 1g of herbal essence (prepared according to example 1) for dispelling wind, removing dampness and relieving pain, and the right side is coated with 1g of vaseline as a control and stuck with a medical adhesive tape. Feeding each guinea pig in cages, applying essence for 24 hr, washing off residual essence with warm water, removing essence for 72 hr, and observing with naked eye and histopathological examination to obtain guinea pig with no red swelling and inflammation.

In the examples, the sources of the raw materials are as follows:

cellulase: the enzyme activity was 80000u/g, Yeshang trade company, Guangzhou.

And (3) pectinase: the enzyme activity is 30000u/g, CAS: 9032-75-1, Shaanxi forest Freund Natural products, Inc.

Cinnamon extract: 80 mesh, 10:1 ratio by conventional water extraction method, Xian Qingzhi biotechnology limited.

And (3) Chinese angelica extract: 80 mesh, 10:1 ratio by conventional water extraction method, Xian Qingzhi biotechnology limited.

Safflower: the origin is as follows: xinjiang, Bozhou city, Juchuntang, Chinese medicinal material marketing Co.

Crocus sativus: the origin is as follows: iran, guang Kuntang agricultural science and technology limited of Yunnan.

Garden balsam stem: the origin is as follows: shandong, Mazhou city and Chinese medicinal material marketing Co.

Camphor bark: the origin is as follows: bei He, the unit of the Xuli flower tea shop in the urban area.

Poloxamer 188: CAS number: 9003-11-6.

The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims

1. The herbal essence capable of dispelling wind, removing dampness and relieving pain is characterized by comprising the following raw materials in percentage by weight: 2-5% of 1, 2-propylene glycol, 1-5% of 1, 3-butanediol, 13-20% of cinnamon extract, 10-20% of safflower extract, 15-20% of crocus sativus extract, 8-15% of garden balsam stem extract, 5-21% of cinnamomum camphora bark extract and 15-25% of angelica sinensis extract;

the safflower extract, the saffron extract, the garden balsam stem extract and the camphor bark extract are prepared by the following steps:

s2, uniformly mixing the traditional Chinese medicine powder, water and the complex enzyme, carrying out enzymolysis for 1.5-2.5 h at the temperature of 40-60 ℃ at the pH of 4.5, and filtering to obtain filtrate A and filter residue a; the mass ratio of the traditional Chinese medicine powder to the water to the compound enzyme is 1 (6-12) to 0.1-0.3; the compound enzyme is formed by mixing cellulase and pectinase according to the mass ratio of 3: 1;

s3, adding the filter residue a and a stabilizer into an ethanol water solution with the mass fraction of 30-80%, extracting for 20-40 min at 40-60 ℃ by ultrasonic wave in cooperation with an electrostatic field, and filtering to obtain a filtrate B; the mass ratio of the filter residue a to the stabilizer to the ethanol water solution is 1 (0.02-0.06) to (10-15); the stabilizer is ferrous sulfate, poloxamer 188 and chitosan according to the mass ratio (1-3): (0.2-0.6): (1-3) mixing;

2. The wind-expelling, dampness-removing and pain-relieving herbal essence according to claim 1, wherein the herbal essence comprises: in the ultrasonic synergistic electrostatic field extraction, the ultrasonic frequency is 20-40 KHz, the ultrasonic power is 100-300W, and the electrostatic voltage is 2.5-15 kV.

3. The wind-expelling, dampness-removing and pain-relieving herbal essence according to claim 1, wherein the herbal essence comprises: the temperature in the reduced pressure evaporation is 40-60 ℃, and the absolute pressure is 0.085-0.095 MPa.

4. The wind-expelling, dampness-removing and pain-relieving herbal essence according to claim 1, wherein the herbal essence comprises: the freezing and condensing is suspended freezing and condensing; wherein the precooling temperature is 5-8 ℃, the stirring speed is 160-200 r/min, and the refrigerant temperature is-12 to-16 ℃.