CN108379294A - The technique that liquid nitrogen frozen polishing prepares donkey placenta freeze-dried powder - Google Patents
The technique that liquid nitrogen frozen polishing prepares donkey placenta freeze-dried powder Download PDFInfo
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- CN108379294A CN108379294A CN201810186166.5A CN201810186166A CN108379294A CN 108379294 A CN108379294 A CN 108379294A CN 201810186166 A CN201810186166 A CN 201810186166A CN 108379294 A CN108379294 A CN 108379294A
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- 239000000843 powder Substances 0.000 title claims abstract description 47
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 239000007788 liquid Substances 0.000 title claims abstract description 23
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000005498 polishing Methods 0.000 title claims abstract description 13
- 238000007710 freezing Methods 0.000 claims abstract description 14
- 230000008014 freezing Effects 0.000 claims abstract description 14
- 238000004108 freeze drying Methods 0.000 claims abstract description 13
- 230000001052 transient effect Effects 0.000 claims abstract description 7
- 238000010894 electron beam technology Methods 0.000 claims abstract description 6
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- 239000000047 product Substances 0.000 abstract description 8
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/015—Preserving by irradiation or electric treatment without heating effect
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/20—Meat products; Meat meal; Preparation or treatment thereof from offal, e.g. rinds, skins, marrow, tripes, feet, ears or snouts
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- A23L13/60—Comminuted or emulsified meat products, e.g. sausages; Reformed meat from comminuted meat product
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
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- A61K9/00—Medicinal preparations characterised by special physical form
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Abstract
The invention discloses the techniques that a kind of liquid nitrogen frozen polishing prepares donkey placenta freeze-dried powder, it is that precooling is cut into donkey placenta thin slice after forming frost donkey placenta at 10 ~ 0 DEG C by fresh donkey placenta, and by donkey placenta thin slice prior to being freezed in liquid nitrogen, it places into freezing mixing and ball milling instrument and is ground into homogenate, it is subsequently placed in freeze drier cold-trap, control condenser temperature is freeze-dried to obtain freeze-drying donkey placenta；Then freeze-drying donkey placenta ultramicro grinding is obtained into the uniform donkey placenta powder of plasmid, packed, after sealing, using Co60Electron beam carries out low dosage transient irradiation to obtain the final product.Donkey placenta tissue can be rapidly and effectively crushed by the present invention using liquid nitrogen frozen polishing within the extremely short time, its large biological molecule is fully discharged into solution, preferably keep the natural activity of large biological molecule, and keep the distinctive color and luster of donkey placenta, the donkey placenta freeze-dried powder of rich in nutrition content has been obtained, while having improved production efficiency and the yield of product.
Description
Technical field
The present invention relates to the preparation methods of a breeding ass placenta freeze-dried powder more particularly to a kind of utilization liquid nitrogen frozen to grind legal system
The technique of standby donkey placenta freeze-dried powder, belongs to Medicines and Health Product technical field.
Background technology
Placenta is that mammality viviparous animal supplies nutrient when pregnant for fetus, allows the particular tissues of embryo growth.Placenta
Have more than 1000 years history in China as tonic,《Compendium of Materia Medica》Middle placenta is referred to as " dried human placenta ", has and " feels at ease to support
Blood, QI invigorating, mend essence, detoxify, enrich blood " effect.Modern study is found：Protein, sugar, micro member contained in placenta tissue
Element, female hormone, cell active factor and peptide matters can improve female incretion adjusting, promotion cell division is proliferated,
Improve immunity of organisms etc..More deep to the placenta research of people, sheep, deer in recent years, corresponding product also compares more.
CN1122198A disclose it is a kind of producing placenta powder with hypothermic lyophilization technology, by the healthy placenta by quarantine (containing each
Kind to human body beneficial to placenta, such as Human plactnta and deer, pig, ox, sheep, horse, rabbit placenta) make cleaning treatment after, merging freezing is dry
Dry machine；Or first by placenta homogenate crush after, crushed material is placed in freeze drier, make temperature be down to -45 DEG C hereinafter, make placenta or
After placenta homogenate is fully lyophilized, powdering is handled such as using ball mill, pulverizer using machinery, is then according to dosage wrapped
Dress, is handled using sterilizing to obtain the final product.With this method prepare placenta powder cooling time is long, efficiency is low, the yield of product compared with
It is low；The all biological macromolecular of placenta tissue cell is not released completely by mechanical treatment placenta or placenta homogenate lyophilized products
The activity of the substances such as immune factor, organized enzyme, humoral regulators, growth factor, the hormone put, and influence placenta.
Donkey placenta contains abundant protein, a variety of amino acid, vitamin and trace element, the panimmunity factor, activity
Enzyme, humoral regulators, growth factor, also the interferon closely related with immune function and with body function run well
Various active polypeptide is the health food with high nutritive value needed for people's life.And at present to donkey placenta and Related product
Comprehensive exploitation on lack go deep into systematic research.CN106259790A discloses a breeding ass placenta health-caring biscuit and its preparation side
It is that raw material makes health-caring biscuit that method, which is with donkey placenta, butter, granulated sugar, egg, Radix Astragali, Radix Codonopsis, Herba Cistanches, flour etc., prepares donkey
Donkey placenta infusion, rear freeze-drying, disinfection, are crushed at sterilization during placenta health care health-caring biscuit, infusion has damaged donkey placenta
In the bioactive substances such as immunoglobulin, hormone.
Invention content
The problem of present invention aims at for existing placenta powder production technology, provide it is a kind of utilize liquid nitrogen frozen
The technique that polishing prepares donkey placenta freeze-dried powder.
Liquid nitrogen frozen polishing of the present invention prepares the technique of donkey placenta freeze-dried powder, be by fresh donkey placenta at -10 ~ 0 DEG C
The donkey placenta that precooling is cut into long 0.5 ~ 1cm after forming frost donkey placenta, width is 0.2 ~ 0.5cm, thickness is 0.2 ~ 0.5cm is thin
Piece, and donkey placenta thin slice is placed into freezing mixing and ball milling instrument and be ground into homogenate and be placed in training prior to freezing 1 ~ 5min in liquid nitrogen
It supports in ware, is subsequently placed in freeze drier cold-trap, control condenser temperature is -68 DEG C ~ -50 DEG C 6 ~ 10h of freezing；Then in vacuum
It spends and is freeze-dried 46 ~ 60h at 1 ~ 20Pa, -70 DEG C ~ -50 DEG C of temperature, obtain freeze-drying donkey placenta；Then will freeze-drying donkey placenta send to
Micronizer crushes, and crosses 60 ~ 80 mesh sieve, obtains the uniform donkey placenta powder of plasmid, packs, after sealing, using Co60Electron beam
Low dosage transient irradiation is carried out to donkey placenta freeze-dried powder, irradiation dose is 11 ~ 13KGy, and irradiation time is 1 ~ 5s to get to natural
Donkey placenta freeze-dried powder.
The donkey placenta is the fresh human placenta containing umbilical cord and bleeding of the umbilicus by inspection and quarantine.
The present invention has the following advantages compared with the prior art：
1, donkey placenta tissue is rapidly and effectively crushed by the present invention using liquid nitrogen frozen polishing within the extremely short time,
Its large biological molecule is fully discharged into solution, obtains also cell Dissolve things inside donkey placenta homogenate, donkey placenta tissue cell is made to exist
It is crushed in very short time, preferably keeps the natural activity of large biological molecule, and keep the distinctive color and luster of donkey placenta（Blood red）；
Meanwhile entire production process operates under cryogenic, maintains whole nutritional ingredients in donkey placenta to the full extent, is immunized
The bioactive substances such as globulin and estrogen have obtained the donkey placenta freeze-dried powder of rich in nutrition content；
2, the present invention effectively increases the production efficiency of product using liquid nitrogen frozen grinding, while improving the yield of product；
3, using Co60Electron beam low dosage transient irradiation donkey milk powder sterilizes, and reduces food loss to the maximum extent, keeps
Food quality extends the food preservation phase.
Specific implementation mode
The preparation process of donkey placenta freeze-dried powder of the present invention will be described further by embodiment below.
1, liquid nitrogen frozen polishing prepares donkey placenta freeze-dried powder
Fresh donkey placenta is placed in -10 DEG C of refrigerator precoolings and forms frost donkey placenta；Frost donkey placenta is placed in microtome,
It is cut into the thin slice for being about 0.5 ~ 1cm, wide about 0.2 ~ 0.5cm, thickness about 0.2 ~ 0.5cm；By thin slice prior to freezing 2min in liquid nitrogen,
It is put into freezing mixing and ball milling instrument rapidly again and is ground into homogenate（About 60 ~ 90s）Homogenate is placed in clean culture dish afterwards；It will contain
There is the culture dish that donkey placenta is homogenized to be put into freeze drier cold-trap, 8h is freezed at -56 DEG C of condenser temperature；Ice will then be filled
Freeze donkey placenta homogenate culture dish to be placed on drying machine drying frame, is 1Pa in vacuum degree, is freeze-dried 48h at -64 DEG C of temperature, obtains
To freeze-drying donkey placenta；Then freeze-drying donkey placenta is sent to micronizer and is crushed, crossed 80 mesh sieve, obtain the uniform donkey placenta of plasmid
Powder, and donkey placenta powder is packed into thermoplastic bag automatic packaging machine sealing and is packed；Finally packaged donkey placenta freeze-dried powder is placed in
On electron accelerator, electron accelerator Co is opened60Electron beam carries out low dosage transient irradiation to donkey placenta freeze-dried powder, irradiates agent
11KGy is measured, irradiation time is 2s to get natural donkey placenta freeze-dried powder.
It is M to weigh to fresh donkey placenta and record its quality1, rear slice, grinding, freeze-drying, ultramicro grinding, sieving obtain
The uniform donkey placenta freeze-dried powder of granular size, it is M that weighing, which records its quality,2, and yield is calculated as follows, and take being averaged three times
Value（It is shown in Table 1）, it is 12.2% to obtain the average yield of donkey placenta powder freeze-dried powder.
Yield=M2 /M1×100%
2, general freezer method prepares donkey placenta freeze-dried powder
Fresh donkey placenta is placed in -10 DEG C of refrigerator precoolings and forms frost donkey placenta；Frost donkey placenta is placed in microtome,
It is cut into and is about 0.5 ~ 1cm, the thin slice that 0.2 ~ 0.5cm, thickness are about 0.2 ~ 0.5cm wide about is placed in clean culture dish；It will
The culture dish for filling donkey placenta thin slice is put into freeze drier cold-trap, is freezed 8h at -56 DEG C of condenser temperature, will then be filled
Frost donkey placenta thin slice ware is placed on drying machine drying frame, is 1Pa in vacuum degree, is freeze-dried 48h at -64 DEG C of temperature, obtains
Donkey placenta is lyophilized；Then freeze-drying donkey placenta is sent to micronizer and is crushed, crossed 80 mesh sieve, obtain the uniform donkey placenta of plasmid
Powder, and donkey placenta powder is packed into thermoplastic bag automatic packaging machine sealing and is packed；Finally packaged donkey placenta freeze-dried powder is placed in
On electron accelerator, electron accelerator Co is opened60Electron beam carries out low dosage transient irradiation to donkey placenta freeze-dried powder, irradiates agent
11KGy is measured, irradiation time is 2s to get to natural donkey placenta freeze-dried powder.
It is M to weigh to fresh donkey placenta and record its qualitya, rear slice, freeze-drying, ultramicro grinding, sieving obtain particle
Uniform donkey placenta freeze-dried powder, it is M that weighing, which records its quality,b, and yield is calculated by above formula, and take average value three times
（It is shown in Table 2）, it is 6.0% to obtain the average yield of donkey placenta powder freeze-dried powder.It can be seen that relative to general freezer seasoning, the present invention
Liquid nitrogen frozen polishing effectively increases the yield of donkey placenta freeze-dried powder（The about 2 times or more of general freezer method）.
3, the test of donkey placenta freeze-dried powder active ingredient
The active ingredient of 1 gained donkey placenta freeze-dried powder of embodiment is surveyed according to the method required by national food safety standard
It is fixed, it the results are shown in Table 3：
4, donkey placental immunity globulin, complement detection
After preserved in -10 DEG C of refrigerators three donkey placentas are placed in 4 DEG C of refrigerator defrostings, 10 portions are chosen in every placenta at random
Position sampling, every part of sample weight 10g, is originally put into sampling in liquid nitrogen, is taken out after freezing l0min, is immediately placed in freezing mixing ball
It is fully ground into homogenate in mill instrument, takes homogenate in low temperature（4℃）5min is centrifuged with 10000r/min in refrigerated centrifuge, takes supernatant
Liquid is put into 1.5ml centrifuge tubes, is tested through the full-automatic plasma protein analyzers of BN prospec, be the results are shown in Table 4：
5, donkey placental immunity globulin, complement detection
After preserved in -10 DEG C of refrigerators three donkey placentas are placed in 4 DEG C of refrigerator defrostings, 10 portions are chosen in every placenta at random
Position sampling, every part of sample weight 10g, is originally put into sampling in liquid nitrogen, is taken out after freezing l0min, is immediately placed in freezing mixing ball
It is fully ground into homogenate in mill instrument, takes homogenate in low temperature（4℃）5min is centrifuged with 10000r/min in refrigerated centrifuge, takes supernatant
Liquid is put into 1.5ml centrifuge tubes, by cobase411 fully automatic electric chemiluminescent analyzers, test progesterone, estradiol III；Through
Cobase601 Electrogenerated chemiluminescent immunoassay instrument tests human chorionic gonadtropin, the results are shown in Table 5：
Claims (4)
1. liquid nitrogen frozen polishing prepare donkey placenta freeze-dried powder technique, be by fresh donkey placenta at -10 ~ 0 DEG C precooling shape
At being cut into donkey placenta thin slice after frost donkey placenta, and it is mixed to be placed into freezing prior to freezing 1 ~ 5min in liquid nitrogen for donkey placenta thin slice
Conjunction ball milling instrument is ground into homogenate and is placed in culture dish, is subsequently placed in freeze drier cold-trap, and control condenser temperature is -68 DEG C
~ -50 DEG C of 6 ~ 10h of freezing；It is then freeze-dried 46 ~ 60h at 1 ~ 20Pa of vacuum degree, -70 DEG C ~ -50 DEG C of temperature, obtains freeze-drying donkey
Placenta；Then freeze-drying donkey placenta is sent to micronizer and is crushed, crossed 60 ~ 80 mesh sieve, obtain the uniform donkey placenta powder of plasmid, wrap
Dress, after sealing, using Co60Electron beam carries out low dosage transient irradiation to donkey placenta freeze-dried powder and is lyophilized to get to natural donkey placenta
Powder.
2. the technique that liquid nitrogen frozen polishing as described in claim 1 prepares donkey placenta freeze-dried powder, it is characterised in that：The donkey tire
Disk is the fresh human placenta containing umbilical cord and bleeding of the umbilicus by inspection and quarantine.
3. the technique that liquid nitrogen frozen polishing as described in claim 1 prepares donkey placenta freeze-dried powder, it is characterised in that：The donkey tire
A length of 0.5 ~ 1cm, the width of disk thin slice be 0.2 ~ 0.5cm, the thin slice that thickness is 0.2 ~ 0.5cm.
4. the technique that liquid nitrogen frozen polishing as described in claim 1 prepares donkey placenta freeze-dried powder, it is characterised in that： Co60Electricity
It is 11 ~ 13KGy that beamlet carries out low dosage transient irradiation dosage to donkey placenta freeze-dried powder, and irradiation time is 1 ~ 5s.
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| CN113413995A (en) * | 2021-06-24 | 2021-09-21 | 广东轻工职业技术学院 | Preparation method of low-crystallinity curcumin |
| CN115125193A (en) * | 2021-07-09 | 2022-09-30 | 广州赛莱拉生物基因工程有限公司 | Animal umbilical cord exosome and extraction method and application thereof |
| CN115671138A (en) * | 2022-12-29 | 2023-02-03 | 江西白凤仙子科技有限公司 | Black-bone chicken lyophilized powder and preparation method thereof |
| WO2023053065A1 (en) * | 2021-09-29 | 2023-04-06 | Osiris Therapeutics, Inc. | Compositions of decidua tissue and uses thereof |
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| CN115671138A (en) * | 2022-12-29 | 2023-02-03 | 江西白凤仙子科技有限公司 | Black-bone chicken lyophilized powder and preparation method thereof |
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