CN1083687C - Method for breeding tetraploid fish - Google Patents
Method for breeding tetraploid fish Download PDFInfo
- Publication number
- CN1083687C CN1083687C CN99115264A CN99115264A CN1083687C CN 1083687 C CN1083687 C CN 1083687C CN 99115264 A CN99115264 A CN 99115264A CN 99115264 A CN99115264 A CN 99115264A CN 1083687 C CN1083687 C CN 1083687C
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- Prior art keywords
- generation
- fishes
- tetraploid
- male
- breeding
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title claims description 12
- 208000035199 Tetraploidy Diseases 0.000 title abstract description 12
- 230000001488 breeding effect Effects 0.000 title abstract description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 230000009027 insemination Effects 0.000 claims description 7
- 241000252210 Cyprinidae Species 0.000 claims description 5
- 241000252233 Cyprinus carpio Species 0.000 abstract description 6
- 208000020584 Polyploidy Diseases 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 abstract description 3
- 210000000349 chromosome Anatomy 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract description 2
- 238000009396 hybridization Methods 0.000 abstract 1
- 210000004940 nucleus Anatomy 0.000 abstract 1
- 230000001568 sexual effect Effects 0.000 abstract 1
- 241000246211 Carassius auratus red var. Species 0.000 description 4
- 241001609213 Carassius carassius Species 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 241000252229 Carassius auratus Species 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000004508 polar body Anatomy 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 210000005132 reproductive cell Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a breeding method for tetraploid fishes, which is used for the breeding of polyploid fishes. The present invention uses red crucian as female parent and uses wild common carp in Xiang river as male parent to obtain F1 generation through artificial fertilization; the male fertile F1 generation is selfed with the F1 generation to obtain F2 generation; big sperms with diploid chromosome in the F2 generation are selected to be combined with ova whose female nucleus is induced by big sperms so as to generate heterogeneous tetraploid fishes. The tetraploid fishes obtained by the present invention have stable heritable character and can propagate naturally, and the tetraploid fishes are peculiar germplasm resources obtained by combining cell engineering and sexual hybridization in the fish breeding.
Description
The present invention relates to the breeding of fish, be specifically related to the cultivation of polyploid fish.
Do the research that the parent hands over each other mutually with crucian and carp report is early arranged.The Japan scholar thinks that the male of crucian carp carp first-filial generation is sterile fully, and Soviet Union scholar also holds same view.Liu Jun etc. are in the crossbreeding experiment of red crucian carp of research and Xiangjiang wild carps, obtained to have obvious heterotic first-filial generation (Hunan crucian carp), simultaneously, discovery Hunan crucian carp is male not to be sterile fully, but part can be educated, can the rate of educating be 4.67%, generally believe that both at home and abroad the carp crucian carp hybridizes male sterile viewpoint thereby revised.The utilization cell engineering is produced the polyploid fish, is used for the production of triploid fish then, is an open question still both at home and abroad.
The present invention is intended to by cell engineering, cultivates the fish tetraploid population of energy natural propagation and stabilization characteristics of genetics.
The main technical schemes that realizes the foregoing invention purpose is as follows:
Be female parent with red crucian carp, Xiangjiang wild carps is a male parent, obtain through artificial insemination on the basis of first-filial generation F1, select male fertile F1 sperm and the mating of female F1 ovum, make F2 generation through artificial insemination,, select to have the chromosomal major part sperm of 2N and induce the F2 thelykaryon through the major part sperm in generation at F2, produce tetraploid F3 generation with artificial insemination method extraordinarily for diplontic ovum.
Be described in further detail the present invention below.
The present invention does maternal red crucian (Carassius auratus L.) and belongs to the type of typically repeatedly laying eggs, and annual late March is to late April, and in about 35～45 days, red crucian carp can lay eggs 2～3 times continuously, and be 12～15 days the blanking time of at every turn laying eggs.Xiangjiang wild carps (Cyprinus carpio L.) as male parent is the wild carp of Xiang River korneforos, and its adaptability is strong, but does not have the people that its various biological properties were carried out research as yet.
Red crucian carp and Xiangjiang wild carps are obtained first-filial generation F1 with artificial insemination method, select in generation 4.67% can educate, be that the more relatively individuality of seminal fluid, sperm quantity is made male parent at F1, with the ovum of its sperm and female individuals, through artificial insemination method, acquisition F2 generation; In F2 generation, select a fairly large number of male of major part sperm (2N) to make male parent, because inducing of major part sperm, make the F2 female individuals carry out maiotic ovum and do not discharge second polar body, so acquisition diplont ovum, adopt artificial insemination method then, diploid sperm is combined with diplont ovum, obtain allotrtraploid fish.
Through the tetraploid fish that said method obtains, the monoploid thelykaryon extraordinarily be nature extraordinarily, do not add external chemistry and physical factor, recombination gene is fully from fish itself, so expresses nature, and is perfectly safe; Just because of this, the tetraploid fish that obtains, be the tetraploid of pure lines, do not have chimeric phenomenon, through the selective examination of drawing materials at random of each generation, chromosome all is that not only ploidy is stable for 4N=200., the growing of morphological feature, reproductive cell, fertilization rate etc. all are normal developments, thus be a kind of stabilization characteristics of genetics, can natural propagation, in Technique in Fishes is cultured, realize obtaining the population of tetraploid fish by biological technique method.
Claims (1)
1, a kind of breeding method of allotrtraploid fish, it is characterized in that with red crucian be female parent, Xiangjiang wild carps is a male parent, obtain first-filial generation F1 with artificial insemination method, select the sperm of 4.67% F1 that can educate for male, use test-tube method, combine with the ovum in F1 generation, obtain F2 generation, in the F2 male in generation, select diplontic major part sperm, and induce thelykaryon ovum extraordinarily through diploid sperm, use test-tube method, diplontic sperm is combined with diplontic ovum, thereby obtain allotrtraploid fish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99115264A CN1083687C (en) | 1999-02-15 | 1999-02-15 | Method for breeding tetraploid fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99115264A CN1083687C (en) | 1999-02-15 | 1999-02-15 | Method for breeding tetraploid fish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1263704A CN1263704A (en) | 2000-08-23 |
| CN1083687C true CN1083687C (en) | 2002-05-01 |
Family
ID=5278261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99115264A Expired - Lifetime CN1083687C (en) | 1999-02-15 | 1999-02-15 | Method for breeding tetraploid fish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1083687C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090360B (en) * | 2010-12-17 | 2012-02-29 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN103875574B (en) * | 2014-04-11 | 2016-05-18 | 湖南师范大学 | Both sexes can be educated the selection of the selection of autotetraploid crucian and the method for building up of strain thereof, triploid fish |
| CN104109652B (en) * | 2014-07-04 | 2016-01-27 | 湖南师范大学 | Artificial induction tetraploid crucian clone and cultural method thereof and application |
| CN111480617A (en) * | 2020-06-01 | 2020-08-04 | 湖南海博水产种业科技有限公司 | Method for preparing amphiprotic fertile tetraploid heterozygous crucian carp and method for preparing sterile triploid heterozygous crucian carp |
| CN115735858B (en) * | 2022-11-24 | 2024-04-19 | 中国科学院水生生物研究所 | Method for efficiently creating new polyploid crucian by sterility synthesis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19632532A1 (en) * | 1996-08-13 | 1998-02-19 | Boehringer Ingelheim Int | Process for the production of mammals with defined genetic properties |
| CN1182531A (en) * | 1997-10-31 | 1998-05-27 | 董文治 | Method for culture of colour crucian carp |
-
1999
- 1999-02-15 CN CN99115264A patent/CN1083687C/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19632532A1 (en) * | 1996-08-13 | 1998-02-19 | Boehringer Ingelheim Int | Process for the production of mammals with defined genetic properties |
| CN1182531A (en) * | 1997-10-31 | 1998-05-27 | 董文治 | Method for culture of colour crucian carp |
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| Publication number | Publication date |
|---|---|
| CN1263704A (en) | 2000-08-23 |
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| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Co-applicant after: Xiangyin Donghu Fishery |
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| COR | Change of bibliographic data |
Free format text: CORRECT: CO-APPLICANT TO: EAST LAKE FISHING GROUND, XIANGYIN COUNTY |
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| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Hunan Xiangyun Biological Co., Ltd. Assignor: East Lake fishing ground of Xiangyin County|Hunan Normal University Contract record no.: 2012430000019 Denomination of invention: Method for breeding tetraploid fish Granted publication date: 20020501 License type: Exclusive License Open date: 20000823 Record date: 20120322 |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20020501 |