CN108345101A - Lighting system and its method illuminated for motor cell shooting - Google Patents
Lighting system and its method illuminated for motor cell shooting Download PDFInfo
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- CN108345101A CN108345101A CN201810041045.1A CN201810041045A CN108345101A CN 108345101 A CN108345101 A CN 108345101A CN 201810041045 A CN201810041045 A CN 201810041045A CN 108345101 A CN108345101 A CN 108345101A
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- led
- led light
- shooting
- light source
- lighting system
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Studio Devices (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Method the present invention provides lighting system and its for motor cell shooting illumination, lighting system include：LED area light source device, it is electrically connected with the LED area light source device, adjust the constant current driver circuit for LED of the electric current driving in the LED area light source device, it is arranged at the aluminum substrate rear, radiator for radiating to LED light, and it is electrically connected with the LED area light source device, control the flash illumination time of LED light in the LED area light source, so that plane fluidic cell micro-imaging controls chip with the matched LED of image, using area source LED as lighting source, effectively reduce the cost of optical lighting system, and the flash time of LED light is controlled using constant current driver circuit for LED and LED control chips, it ensure that the image quality of plane streaming cell imaging system.
Description
Technical field
The present invention relates to micro-imaging technique field more particularly to a kind of illumination systems of plane FCM analysis instrument
System and its method illuminated for motor cell shooting.
Background technology
Plane fluidic cell micro-imaging technique is the cell or biologic grain for being in quick straight line flow regime
Carry out multi-parameter, the technology of fast quantitative analysis and sorting.Optical system, fluid path channel and data analysis system are plane streamings
The chief component of cell micro-imaging technique, and wherein optical unit is then entire plane fluidic cell micro-imaging technique
Basis.
However, existing plane FCM analysis instrument price is high, therefore limit the universal and utilization of this kind of instrument.
Common FCM analysis instrument uses high frequency xenon lamp spot light at this stage, although the lighting system optical texture is more
Simply, but make entire detecting instrument as its high cost of the high frequency xenon lamp of light source and relatively short service life
Popularization and application receive certain limitation.
Therefore, the prior art is up for further improving.
Invention content
It is thin it is an object of the invention to provide a kind of plane streaming to the user in view of above-mentioned shortcoming in the prior art
The lighting system of born of the same parents' detector and its method illuminated for motor cell shooting, overcome plane fluidic cell inspection in the prior art
Survey instrument leads to expensive defect using high frequency xenon lamp.
The present invention provides first embodiment be a kind of lighting system of plane FCM analysis instrument, wherein be used for
It is illuminated for plane FCM analysis instrument when carrying out motor cell image taking, including：
It is provided with the LED area light source device of aluminum substrate, LED light is installed on the aluminum substrate；
Constant current driver circuit for LED is electrically connected with the LED area light source device, and the electric current adjusted in the LED area light source device drives
It is dynamic；
Radiator is arranged at the aluminum substrate rear, for radiating to LED light；
LED controls chip, is electrically connected with the LED area light source device, controls the flash illumination of LED light in the LED area light source
Time so that plane fluidic cell micro-imaging is matched with image.
Optionally, the LED area light source device includes：Multi-disc lens group and the optical filter being placed in inside lens group；
The multi-disc lens group and optical filter are arranged at the light light incident side of LED light, shine respectively the light that LED light is sent out
Bright adjusting and filtering.
Optionally, the radiator includes fan, fan net and cooling fin；The radiator, which is located at, is set to illumination
The tail end of caliber, shape are circular ring type, and the LED area light source device is located at the heat sink interior.
Optionally, the LED control chips control the flash illumination time of LED light in LED area light source according to following formula：
T=T1-T2;
Wherein, T is the flash illumination time of LED light in LED area light source, and T1 is the imaging sensor minimum exposure time, and T2 is thin
The minimum exposure time is shot when born of the same parents move.
Optionally, the multi-disc lens group is four-piece type illuminating lens group, and the four-piece type illuminating lens group includes：It is poly-
Light microscopic group and light harvesting microscope group.
The present invention provides second embodiment be a kind of lighting system for motor cell shooting illumination method,
In, include the following steps：
It receives shooting camera and opens shooting moving object signal；
The flash illumination time of control shooting camera exposure time and LED light, while shooting camera and obtaining motor cell image；
After exposure, exits exposure mode and close LED light, and export motor cell image.
Optionally, further include：
When calculating the flash illumination time of LED light, and the flash illumination of LED light initialized according to the calculated flash illumination time
Between parameter.
Optionally, the step of flash illumination time for calculating LED light includes：
According to the pixel dimension of the movement velocity and selected imaging sensor of shooting motor cell, when calculating shooting minimum exposure
Between；
The flash illumination time of LED light is calculated according to the shooting minimum exposure time.
Optionally, the step of flash illumination time for controlling shooting camera exposure time and LED light further includes：
After receiving the shooting signal that shooting camera is sent out, exposure timer is opened, into exposure mode, meanwhile, it opens and dodges
Light lamp timer, into delay ignition mode；
Timer is closed after reaching the shooting minimum exposure time, exits exposure mode, and reaching preset LED light delay
After time, lightening LED lamp is controlled;
After reaching the flash illumination time of LED light, LED light is extinguished in control.
Optionally, the control lightening LED lamp and control extinguish LED light the step of further include：
Constant current driver circuit for LED is that high level control LED light is lighted by inputting pwm signal, is low electricity by inputting pwm signal
Flat control LED light is extinguished.
Advantageous effect, a kind of method the present invention provides lighting system and its for motor cell shooting illumination, including：
LED area light source device is electrically connected with the LED area light source device, adjusts the electric current driving in the LED area light source device
Constant current driver circuit for LED, setting are at the aluminum substrate rear, the radiator for radiating to LED light, and with it is described
LED area light source device is electrically connected, and controls the flash illumination time of LED light in the LED area light source so that plane fluidic cell is aobvious
Micro- imaging effectively reduces illumination optical with the matched LED controls chip of image using area source LED as lighting source
The cost of system, and the flash time of LED light is controlled using constant current driver circuit for LED and LED control chips, ensure
The image quality of plane streaming cell imaging system.
Description of the drawings
Fig. 1 is the structural schematic diagram of lighting system of the present invention；
Fig. 2 is the sectional view of lighting system of the present invention；
Fig. 3 is the principle schematic of LED light flash of light control in illumination system embodiment of the present invention；
Fig. 4 is the oscillogram of the control signal of LED light flash of light control in illumination system embodiment of the present invention；
Fig. 5 is method and step flow chart of the lighting system of the present invention for motor cell shooting illumination；
Fig. 6 is the step flow chart of the concrete application embodiment of the method for the invention.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer and more explicit, develop simultaneously embodiment pair referring to the drawings
The present invention is further described.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, and do not have to
It is of the invention in limiting.
The present invention provides first embodiment be a kind of lighting system of plane FCM analysis instrument, as shown in Figure 1,
The lighting system is used to illuminate when carrying out motor cell image taking for plane FCM analysis instrument, in conjunction with shown in Fig. 2,
The lighting system includes：
It is provided with the LED area light source device of aluminum substrate, LED light is installed on the aluminum substrate；The quantity of the LED light is one
It is a.
The LED area light source device includes:LED area light source 12.In the present invention high frequency point light source is replaced using LED area light source
Xenon lamp, the light source of the inexpensive LED illumination System as plane fluidic cell micro-imaging.
Constant current driver circuit for LED is electrically connected with the LED area light source device, adjusts the electricity in the LED area light source device
Stream driving.
Since lighting power may be used in the specific implementation less than high frequency point light source xenon lamp in the lighting power of LED
The controllable led constant-current drive circuits of small low-cost of maximum 30W are adjusted by peripheral hardware I/O interface voltage and realize adjusting continuous current
Drive size.
Radiator 11 is arranged at the aluminum substrate rear, for radiating to LED light；Specifically, the heat dissipation dress
It is set to fan component 13, is radiated to LED light using air-cooled.
LED controls chip, is electrically connected with the LED area light source device, controls the flash of light of LED light in the LED area light source
Lighting hours so that plane fluidic cell micro-imaging is matched with image.
Due to when shooting moving object, if to be more than a pixel wide for object image displacement distance within the time for exposure
Degree, then final image has trailing phenomenon, and image displacement distance is bigger, then trailing phenomenon is more serious.However all images
All there are one minimum exposure time period values for sensor, and limiting exposure is smaller, and imaging sensor cost is higher.In fluid stream
When speed is relatively low, being taken pictures using common higher frequency camera can meet the requirements；However for the fluid of high flow rate, only it is used only common
Higher frequency camera be inadequate, this just needs to be controlled the lighting mode of LED light source.
Therefore the electric current of LED area light source is controlled using constant current driver circuit for LED and LED control chips in the present invention
System so that LED carries out flash illumination according to the scheduled flash illumination time, to realization acquirement and high frequency xenon lamp spot light
Identical illuminating effect.
Specifically, optionally, the flash of light that the LED control chips control LED light in LED area light source according to following formula is shone
The bright time：
T=T1-T2;
Wherein, T is the flash illumination time of LED light in LED area light source, and T1 is the imaging sensor minimum exposure time, and T2 is thin
The minimum exposure time is shot when born of the same parents move.
In a particular embodiment, driving circuit of high-power LED lamp can be controlled by LED triggers, lightening LED lamp shines
It is bright, and realized the concentration of illumination path energy by lighting system and be uniformly distributed, LED heat dissipations are realized by fan component.
Further, lighting system of the invention is using LED area light source as lighting source, using kohler's illumination mode,
By two groups of multi-disc lens groups（Optically focused microscope group sum aggregate light microscopic group）Realize illumination uniformity；By the way of adding optical filter, effectively
The stray light reduced into optical system, improve the image quality of plane streaming cell imaging system.
Preferably, in order to ensure that better imaging effect, the LED area light source device include：It multi-disc lens group 14 and sets
Optical filter 11 inside lens group；
The multi-disc lens group 14 and optical filter 11 are arranged at the light light incident side of LED light, the light that LED light is sent out respectively into
Row illumination adjustments and filtering.
Preferably, the multi-disc lens group is four-piece type illuminating lens group, and the four-piece type illuminating lens group includes：It is poly-
Light microscopic group and light harvesting microscope group.The optical filter is placed in inside optically focused microscope group, for filtering stray light, to improve image quality.
The purpose of optically focused microscope group is to keep sample good with homogeneous and bright color reducibility, optically focused microscope group also has convergence of rays to sample
Numerical aperture is adjusted, resolution ratio and the depth of field are influenced.Using aspherical light harvesting microscope group, lighting system light collecting light ability is improved.
For the ease of radiating to LED light, the radiator includes fan, fan net and cooling fin；The heat dissipation
Device is located at the tail end for being set to illumination caliber, and shape is circular ring type, and the LED area light source device is located at the radiator
It is internal.
In conjunction with shown in Fig. 3 and Fig. 4, the constant current driver circuit for LED is that high level controls LED light by inputting pwm signal
It lights, is extinguished for low level control LED light by inputting pwm signal.
In a particular embodiment, a kind of small low-cost LED constant current driving electricity of lighting power maximum 30W may be used
Road, using the LED light of low cost high power patch type, constant current driver circuit for LED is designed to the short time（Ns ranks rising edge,
Failing edge）The constant-current source driving for being up to several Ampere currents can be provided, driving current size can be linear according to external input voltage
Ratio controls, and the lamp bright time can control according to external input pwm signal, and high level is lighted, and low level lamp goes out.Using controllable defeated
Inbound port can constitute accurately closed-loop control with camera.
The present invention provides second embodiment be a kind of lighting system for motor cell shooting illumination method, in conjunction with
Shown in Fig. 5, include the following steps：
Step S1, it receives shooting camera and opens shooting moving object signal；
Step S2, the flash illumination time of control shooting camera exposure time and LED light, while shooting camera and obtaining motor cell
Image；
Step S3, it after exposure, exits exposure mode and closes LED light, and export motor cell image.
Preferably, before the step of above-mentioned reception shooting camera opens shooting moving object signal, further include：
When calculating the flash illumination time of LED light, and the flash illumination of LED light initialized according to the calculated flash illumination time
Between parameter.
Specifically, the step of flash illumination time for calculating LED light, includes：
According to the pixel dimension of the movement velocity and selected imaging sensor of shooting motor cell, when calculating shooting minimum exposure
Between；
The flash illumination time of LED light is calculated according to the shooting minimum exposure time.
Specifically, the step of control shooting camera exposure time and flash illumination time of LED light, further includes：
After receiving the shooting signal that shooting camera is sent out, exposure timer is opened, into exposure mode, meanwhile, it opens and dodges
Light lamp timer, into delay ignition mode；
Timer is closed after reaching the shooting minimum exposure time, exits exposure mode, and reaching preset LED light delay
After time, lightening LED lamp is controlled;
After reaching the flash illumination time of LED light, LED light is extinguished in control.
Optionally, the control lightening LED lamp and control extinguish LED light the step of further include：
Constant current driver circuit for LED is that high level control LED light is lighted by inputting pwm signal, is low electricity by inputting pwm signal
Flat control LED light is extinguished.
In order to which method provided by the invention is described in detail, below by when it is embodied the step of for, do
Further instruction.As shown in fig. 6, LED control technologies scheme of the present invention includes the following steps：
Step A initializes flash lamp delay time parameter.According to the movement velocity of shooting object image and selected image sensing
The pixel dimension of device calculates required minimum exposure time when shooting, then the calculating of final flash lamp delay time parameter is public
Formula is：
M- actual needs minimum exposure time when flash lamp delay time=imaging sensor minimum exposure；
Step B waits for camera external trigger shooting signal to arrive；
Step C, controls camera exposure and LED flash is lighted in delay；
Step C1 opens exposure timer and closes timer after reaching the minimum exposure time into exposure mode, exits exposure
Optical mode；
Step C2 opens flash lamp timer, into delay ignition mode, after the delay time for reaching specified, ability starting point
Intense flash lamp；
Step D exits exposure mode, and closes flash lamp；
Step E, camera start to export image.
The present invention provides a kind of method that moving object is shot under the conditions of inexpensive micro-imaging, and new image pickup method solves
The image shift compensation problem that moving object is shot under existence conditions.
A kind of method the present invention provides lighting system and its for motor cell shooting illumination, including：LED area light source
Device is electrically connected with the LED area light source device, adjusts the LED constant current driving of the electric current driving in the LED area light source device
Circuit, setting is in the aluminum substrate rear, the radiator for radiating to LED light, and is filled with the LED area light source
Electrical connection is set, controls the flash illumination time of LED light in the LED area light source so that plane fluidic cell micro-imaging and object
The cost of optical lighting system is effectively reduced using area source LED as lighting source as matched LED controls chip,
And the flash time of LED light is controlled using constant current driver circuit for LED and LED control chips, ensure that plane streaming
The image quality of cell imaging system.
It, can according to the technique and scheme of the present invention and its hair it is understood that for those of ordinary skills
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect range.
Claims (10)
1. a kind of lighting system of plane FCM analysis instrument, which is characterized in that for existing for plane FCM analysis instrument
Illuminated when motor cell image taking, including：
It is provided with the LED area light source device of aluminum substrate, LED light is installed on the aluminum substrate；
Constant current driver circuit for LED is electrically connected with the LED area light source device, and the electric current adjusted in the LED area light source device drives
It is dynamic；
Radiator is arranged at the aluminum substrate rear, for radiating to LED light；
LED controls chip, is electrically connected with the LED area light source device, controls the flash illumination of LED light in the LED area light source
Time so that plane fluidic cell micro-imaging is matched with image.
2. the lighting system of plane FCM analysis instrument according to claim 1, which is characterized in that the faces LED light
Source device includes：Multi-disc lens group and the optical filter being placed in inside lens group；
The multi-disc lens group and optical filter are arranged at the light light incident side of LED light, shine respectively the light that LED light is sent out
Bright adjusting and filtering.
3. the lighting system of plane FCM analysis instrument according to claim 1, which is characterized in that the radiator
Including fan, fan net and cooling fin；The radiator is located at the tail end for being set to illumination caliber, and shape is circular ring type,
The LED area light source device is located at the heat sink interior.
4. the lighting system of plane FCM analysis instrument according to claim 1, which is characterized in that the LED controls
Chip controls the flash illumination time of LED light in LED area light source according to following formula：
T=T1-T2;
Wherein, T is the flash illumination time of LED light in LED area light source, and T1 is the imaging sensor minimum exposure time, and T2 is thin
The minimum exposure time is shot when born of the same parents move.
5. the lighting system of plane FCM analysis instrument according to claim 2, which is characterized in that the multi-disc lens
Group is four-piece type illuminating lens group, and the four-piece type illuminating lens group includes：Optically focused microscope group and light harvesting microscope group.
6. a kind of method of lighting system as described in claim 1 for motor cell shooting illumination, which is characterized in that including
Following steps：
It receives shooting camera and opens shooting moving object signal；
The flash illumination time of control shooting camera exposure time and LED light, while shooting camera and obtaining motor cell image；
After exposure, exits exposure mode and close LED light, and export motor cell image.
7. method of the lighting system according to claim 6 for motor cell shooting illumination, which is characterized in that also wrap
It includes：
When calculating the flash illumination time of LED light, and the flash illumination of LED light initialized according to the calculated flash illumination time
Between parameter.
8. method of the lighting system according to claim 7 for motor cell shooting illumination, which is characterized in that the meter
Calculate LED light the flash illumination time the step of include：
According to the pixel dimension of the movement velocity and selected imaging sensor of shooting motor cell, when calculating shooting minimum exposure
Between；
The flash illumination time of LED light is calculated according to the shooting minimum exposure time.
9. method of the lighting system according to claim 7 for motor cell shooting illumination, which is characterized in that the control
System shooting the camera exposure time and LED light the flash illumination time the step of further include：
After receiving the shooting signal that shooting camera is sent out, exposure timer is opened, into exposure mode, meanwhile, it opens and dodges
Light lamp timer, into delay ignition mode；
Timer is closed after reaching the shooting minimum exposure time, exits exposure mode, and reaching preset LED light delay
After time, lightening LED lamp is controlled;
After reaching the flash illumination time of LED light, LED light is extinguished in control.
10. method of the lighting system according to claim 9 for motor cell shooting illumination, which is characterized in that described
It controls lightening LED lamp and controls the step of extinguishing LED light and further include：
Constant current driver circuit for LED is that high level control LED light is lighted by inputting pwm signal, is low electricity by inputting pwm signal
Flat control LED light is extinguished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810041045.1A CN108345101A (en) | 2018-01-16 | 2018-01-16 | Lighting system and its method illuminated for motor cell shooting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810041045.1A CN108345101A (en) | 2018-01-16 | 2018-01-16 | Lighting system and its method illuminated for motor cell shooting |
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| Publication Number | Publication Date |
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| CN108345101A true CN108345101A (en) | 2018-07-31 |
Family
ID=62960367
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810041045.1A Pending CN108345101A (en) | 2018-01-16 | 2018-01-16 | Lighting system and its method illuminated for motor cell shooting |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115580782A (en) * | 2022-11-09 | 2023-01-06 | 合肥安迅精密技术有限公司 | Light source brightness control method and system for linear array camera illumination and storage medium |
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| CN115580782A (en) * | 2022-11-09 | 2023-01-06 | 合肥安迅精密技术有限公司 | Light source brightness control method and system for linear array camera illumination and storage medium |
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Application publication date: 20180731 |