CN108342441B - One kind relieves fatigue and oxidation resistant Yak Bone Protein peptide and preparation method - Google Patents
One kind relieves fatigue and oxidation resistant Yak Bone Protein peptide and preparation method Download PDFInfo
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- CN108342441B CN108342441B CN201810162657.6A CN201810162657A CN108342441B CN 108342441 B CN108342441 B CN 108342441B CN 201810162657 A CN201810162657 A CN 201810162657A CN 108342441 B CN108342441 B CN 108342441B
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 105
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 95
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 230000003647 oxidation Effects 0.000 title description 4
- 238000007254 oxidation reaction Methods 0.000 title description 4
- 239000007788 liquid Substances 0.000 claims abstract description 61
- 239000004365 Protease Substances 0.000 claims abstract description 47
- 108091005804 Peptidases Proteins 0.000 claims abstract description 38
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 38
- 230000003064 anti-oxidating effect Effects 0.000 claims abstract description 18
- 238000004007 reversed phase HPLC Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 15
- 150000001875 compounds Chemical class 0.000 claims abstract description 13
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Biophysics (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
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- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of with relieving fatigue and the preparation method of the Yak Bone Protein peptide of anti-oxidation function and the protein peptides being prepared, its main improvement is, the pretreatment such as high-frequency ultrasonic and super-voltage micro jet is carried out to Yak Bone Protein liquid, under conditions of not adding any bronsted lowry acids and bases bronsted lowry, stepwise discretization is carried out using a variety of compound proteases, it is isolated by UF membrane, gel separation and reversed-phase HPLC isolation technics.Yak Bone Protein peptide preparation method of the invention is simple, does not have addition acid or alkali to carry out the adjustment of pH in whole process, and product keeps preferable functional characteristic, is easier to realize industrialization production.
Description
Technical field
The present invention relates to functional protein manufacture fields, and in particular to Yak Bone be raw material production have relieve fatigue and
The protein peptides of anti-oxidation function and the production method of protein peptides.
Background technique
Fatigue is that body is mental or physical strength reaches a kind of normal physiological phenomenon necessarily occurred when certain phase.
It is to prevent body from a kind of aversion response caused by the excessive failure function of life-threatening occurs.Antifatigue peptide
The active and interior environment that amino acid and energy substance necessary to human body can be not only provided, are able to maintain that body enzyme again simultaneously
Stabilization, various functions characteristic, the antifatigue peptide such as enhance oxidation resistance, prevent neurotransmitter unbalance and mainly pass through enhancing muscle
Strength improves body movement endurance, to eliminate or postpone the generation of fatigue rapidly, help restores and enhance physical strength.In addition, anti-
Lcf polypeptide is easily quickly absorbed by human body, no matter from infiltration rate or biological function, for being engaged in physical strength, mental, body
It educates for the crowd of movement, antifatigue peptide will all become its important functional food ingredient.
Inhibit in country's patent about the anti-oxidant of protein peptides, ACE at present and the patent application of function of blood sugar reduction is more, but
It is seldom about anti-fatigue effect protein peptides patent application.Especially not only with protein peptides report antifatigue but also with anti-oxidation function
Road is seldom.Using Yak Bone as raw material, the patent document with antifatigue and anti-oxidation function protein peptides and correlative study are prepared
Report yet there are no.
Summary of the invention
The object of the present invention is to provide a kind of with antifatigue and anti-oxidation function Yak Bone Protein peptide, the protein peptides
It is prepared by the following method:
1) Yak Bone is subjected to boiling at high temperature, obtains ox bone protein liquid；
2) ultrasound is carried out to the ox bone protein liquid and dynamic super-voltage micro jet pre-processes, change ox in ox bone protein liquid
The institutional framework of bone protein；
3) enzymatic hydrolysis is carried out in two steps to pretreated ox bone protein liquid with protease, the protein liquid after must digesting；
4) hyperfiltration treatment is carried out to the protein liquid after the enzymatic hydrolysis with the ultrafiltration membrane of 3000 dalton, obtained containing protein peptides
Solution；
5) solution containing protein peptides is separated by gel column, then utilizes reversed-phase high performance liquid chromatography will
Separated by substance in first isolated eluting peak of the gel column, collect isolate to get；
In the step 3), used enzyme is alkali protease, neutral proteinase and pancreas during the first step digests
The complex enzyme one of protease；Used enzyme is the complex enzyme of papain and flavor protease during second step digests
Two.
The present invention is directed to the material characteristic of Yak Bone, by carrying out high-frequency ultrasonic, super-voltage micro jet etc. to Yak Bone
Pretreatment carries out stepwise discretization using five kinds of compound proteases under the conditions of not adding any bronsted lowry acids and bases bronsted lowry, by UF membrane, coagulates
Glue separation and reversed-phase HPLC isolation technics, develop with specific amino acids polypeptide sequence
(Val-Ile-Glu-Ala-Val-Val-Gln-Ser-Val, Asn-Phe-Ile-Lys-Gln-Ser；
Tyr-Leu-Glu-Glu-Lys, Ala-Gly-Glu-Val-Leu) antifatigue and anti-oxidation function Yak Bone
Protein peptides establish the preparation method of a set of multi-functional Yak Bone Protein peptide being simple and efficient.
Preferably, the isolate is isolated peptide solution.
Preferably, in the step 3), the mass ratio of alkali protease, neutral proteinase and trypsase is 1~2:1:
1；The mass ratio of papain and flavor protease is 1:1~2.
Preferably, the actual conditions digested in the step 3) are as follows: adjust albumen in pretreated ox bone protein liquid
Content is 6~10%, and during the first step digests, the additive amount of the complex enzyme one is the weight of ox bone albumen dry matter
0.4~1.0%, during second step digests, the additive amount of the complex enzyme two is the 0.3 of the weight of ox bone albumen dry matter
~0.6%.
Preferably, first step enzymatic hydrolysis is digests 0.5~1.5h under the conditions of 45~60 DEG C, and second step enzymatic hydrolysis is 45~55
1.0~2.0h is digested under the conditions of DEG C.
It is further preferred that the first step enzymatic hydrolysis under the conditions of 50~60 DEG C digest 1.0~1.5h, second step enzymatic hydrolysis for
1.0~2.0h is digested under the conditions of 50~55 DEG C.
Preferably, in the step 1), before carrying out boiling to the Yak Bone, it is broken into skeletal grain；
Preferably, the concrete operations of the boiling are as follows: it is incorporated as 1.0~3.0 times of ox bone particle weight of water, 121~
It is handled 2~5 hours under the conditions of 131 DEG C, filtrate is obtained after centrifugal filtration, degreasing obtains ox bone protein liquid.
It is further preferred that the concrete operations of the degreasing are that filtrate is cooled to 15~30 DEG C, pass through liquid separation tank degreasing.
Preferably, the pretreated actual conditions of ultrasound are to adjust the temperature of the ox bone protein liquid to 65-75
DEG C, 15~25min is handled under conditions of frequency is 50~80kH.Pass through ultrasonic treatment, thus it is possible to vary the knot of tissue of protein
Structure improves yak albumen to the sensibility of enzyme, reduces the usage amount of enzyme.
Preferably, the pretreated concrete operations of the super-voltage micro jet are to first pass through at the pre- homogeneous of homogenizer of 20MPa
Reason is primary, then carries out super-voltage micro jet processing, handles 2~3 times under conditions of 140~220Mpa of pressure.It can excessively above-mentioned place
Yak albumen can be improved to the sensibility of enzyme in reason, reduces the usage amount of enzyme.
Preferably, gel column described in the step 5) is Sephadex G-15, and eluting peak is detected in 280nm.
The polypeptide that Sephadex G-15 can generate enzymatic hydrolysis carries out preliminary separation.
Preferably, the concrete operations that RP-HPLC reversed-phase high performance liquid chromatography is separated are 5~10mL/ of flow rate of mobile phase
min；Detection wavelength 220nm；
Elution program are as follows: mobile phase A is pure water, and Mobile phase B is the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；
The volume fraction of 0~5min Mobile phase B is 0%, and the volume fraction of 5~40min Mobile phase B is increased to from 0%
The volume fraction of 35%, 40~45min Mobile phase B is increased to 95% from 35%, the volume fraction of 45~50min Mobile phase B from
95% is reduced to 0%.
The preparation method of the acetonitrile solution of the trifluoroacetic acid of above-mentioned volume fraction 0.1% are as follows: by volume, in acetonitrile
It is added to the trifluoroacetic acid of its volume 0.1%.
Preferably, according to above-mentioned separation method, the isolated peptide solution of 12~14min is collected.
As a preferred option, the method for the present invention includes following steps:
1) Yak Bone is broken into skeletal grain, is incorporated as 1.0~3.0 times of ox bone particle weight of water, in 121~131 DEG C of items
It is handled 2~5 hours under part, filtrate is obtained after centrifugal filtration, degreasing obtains ox bone protein liquid；
2) temperature of the ox bone protein liquid is adjusted to 65~75 DEG C, handles 15 under conditions of frequency is 50~80kH
~25min；It is primary using the pre- homogenization of the homogenizer of 20MPa, then surpassed under conditions of 140~220Mpa of pressure
High pressure microjet is handled 2~3 times, changes the institutional framework of ox bone albumen in ox bone protein liquid；
3) two steps of pretreated ox bone protein liquid substep are digested with protease, the protein liquid after must digesting；The
Used enzyme is the complex enzyme one of alkali protease, neutral proteinase and trypsase, alkaline egg during one step digests
The mass ratio of white enzyme, neutral proteinase and trypsase is 1~2: 1: 1；Used enzyme is wood during second step digests
The mass ratio of the complex enzyme two of melon protease and flavor protease, papain and flavor protease is 1:1~2；Enzymatic hydrolysis
Actual conditions are that first step enzymatic hydrolysis is digests 1.0~1.5h under the conditions of 50~60 DEG C, and second step enzymatic hydrolysis is in 50~55 DEG C of item
1.0~2.0h is digested under part.；
4) hyperfiltration treatment is carried out to the protein liquid after the enzymatic hydrolysis with the ultrafiltration membrane of 3000 dalton, obtained containing protein peptides
Solution；
It 5) is that Sephadex G-15 is separated by gel column by the solution containing protein peptides, eluting peak exists
280nm is detected, and the obtained protein peptides in first eluting peak are dried, and then uses reversed-phase high performance liquid chromatography
It is separated, isolated concrete operations are as follows: 5~10mL/min of flow rate of mobile phase；Detection wavelength 220nm；Elution program are as follows: flowing
Phase A is pure water, and Mobile phase B is the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；The volume fraction of 0~5min Mobile phase B
Be 0%, the volume fraction of 5~40min Mobile phase B from 0% to 35%, the volume fraction of 40~45min Mobile phase B from 35% to
95%, 45~50min Mobile phase B collect the isolated albumen peptide solution of 12-14min from 95% to 0%.
The protein peptides that it is another object of the present invention to protect the method for the invention to be prepared.
Preferably, the protein peptides are the albumen peptide solution being directly isolated to obtain or the albumen peptide solution are dried
The protein peptide powder obtained afterwards.
It is measured by main component of the LC-MS/MS to bone collagen peptide, amino acid sequence Val-Ile-
Glu-Ala-Val-Val-Gln-Ser-Val, Asn-Phe-Ile-Lys-Gln-Ser；Tyr-Leu-Glu-Glu-Lys, Ala-
The content of the peptide of Gly-Glu-Val-Leu is 55~62%.
Final object of the present invention is that protection protein peptides of the present invention are added as food, health care product or drug
Application in agent.
The invention has the following beneficial effects:
1) the Yak Bone Protein peptide preparation method developed of the present invention is simple, in whole process without addition acid or alkali into
The adjustment of row pH, product keep preferable functional characteristic, are easier to realize industrialization production.
2) protein peptides developed have preferable antifatigue and anti-oxidation function.
3) present invention utilizes the ox bone protein liquid of specific frequency ultrasonic wave combination super-voltage micro jet technical treatment, Ke Yiming
The aobvious ox bone albumen that improves reduces the usage amount of enzyme to the sensibility of enzyme.
4) product safety developed, the present invention use numerous food grade compound protease (alkali protease, neutral protein
Enzyme, trypsase, papain and flavor protease), through in a mild condition, being digested by appropriateness and obtaining specified molecular weight
With the ox bone protein peptides of peptide composition, any additive is not added, is 100% ox bone protein peptides.
5) protein peptides that the present invention develops have good flavor, can be widely used in special procuring food and nutraceutical.
6) ratio of present invention exploitation peptide of the bone protein peptide middle-molecular-weihydroxyethyl less than 1000 is 90% or more, is had relatively specific
The composition of peptide.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
The present embodiment is related to a kind of preparation method with antifatigue and anti-oxidation function Yak Bone Protein peptide, including such as
Lower step:
1) fresh 100 grams of Yak Bone of freezing are selected, is cleaned with the clean water for meeting sanitary standard for drinking water, is used after ox bone cleaning
Ox bone is broken into skeletal grain by ox bone pulverizer, and the water that 2 times of ox bone particle total amount is added is handled 5 hours under the conditions of 121 DEG C, by from
The ox bone cooking liquor for slag of boning is obtained by filtration in the heart, is cooled to 20 DEG C, by liquid separation tank degreasing, obtains ox bone protein liquid.
2) the Yak Bone Protein liquid that will 1) obtain, temperature are adjusted to 65 DEG C, using supersonic generator through ultrasonic wave (frequency
For 80kH) processing 15 minutes, it is primary using the pre- homogenization of the homogenizer of 20MPa, then under conditions of pressure 160Mpa into
Row super-voltage micro jet is handled 2 times.
3) adjusting protein content in Yak Bone Protein liquid is 8%, according to the weight of protein by weight 0.8% in Yak Bone Protein liquid
Measure percentage compound protease be added and carry out stepwise discretization, the first step using 0.5% compound protease one (alkali protease, in
Property protease and trypsase composition, mass ratio between them be 2: 1: 1), enzyme digestion reaction 1.5h is carried out under the conditions of 50 DEG C；
Then carrying out second step, (papain and flavor protease form, the quality between them using 0.3% compound protease two
Than enzyme digestion reaction 1.0h being carried out under the conditions of 55 DEG C for 1:2)；10 minutes are kept the temperature at 95 DEG C, is cooled to room temperature, enzymatic hydrolysis is added
The active carbon of liquid weight 0.2% is separated in enzymolysis liquid by diatomite, and separating liquid is collected；
It 4) is the ceramic membrane ultrafitration of 10000 dalton using aperture by step (3) resulting separating liquid, it is first that molecular weight is small
Albumen and peptide separation in 10000 come out, then with aperture be 3000 dalton film by molecular weight less than 3000 dalton
Protein peptides are separated.
5) taking molecular weight is the protein peptides liquid less than 3000, is separated using Sephadex G-15 gel, and eluent is to go
Ionized water, eluting peak are detected at 280nm, collect the 1st eluting peak；By being concentrated, being freeze-dried, obtain by gel
Isolated ox bone protein peptides；1 separation, reversed-phase high performance liquid chromatography separation are carried out with RP-HPLC reversed-phase high performance liquid chromatography again
Concrete operations are as follows: 5~10mL/min of flow rate of mobile phase；Detection wavelength 220nm；Elution program are as follows: mobile phase A is pure water, stream
Dynamic phase B is the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；The volume fraction of 0~5min Mobile phase B be 0%, 5~
The volume fraction of 40min Mobile phase B from 0% to 35%, the volume fraction of 40~45min Mobile phase B from 35% to 95%, 45~
50min Mobile phase B from 95% to 0%, take 12-14min collect peptide solution to get.
6) peptide solution for obtaining step (5) is by concentration, freeze-drying, obtains having and relieves fatigue and anti-oxidation function
Bone collagen Gly-His-Lys.It is measured by main component of the LC-MS/MS to bone collagen peptide, amino acid sequence are as follows:
Val-Ile-Glu-Ala-Val-Val-Gln-Ser-Val, Asn-Phe-Ile-Lys-Gln-Ser；Tyr-Leu-
The content of the peptide of Glu-Glu-Lys, Ala-Gly-Glu-Val-Leu is 57%.
Embodiment 2
The present embodiment is related to a kind of preparation method with antifatigue and anti-oxidation function Yak Bone Protein peptide, including such as
Lower step:
1) fresh 500 grams of Yak Bone of freezing are selected, is cleaned with the clean water for meeting sanitary standard for drinking water, is used after ox bone cleaning
Ox bone is broken into skeletal grain by ox bone pulverizer, and the water that 1 times of ox bone particle total amount is added is handled 3 hours under the conditions of 130 DEG C, by from
The ox bone cooking liquor for slag of boning is obtained by filtration in the heart, is cooled to 30 DEG C, by liquid separation tank degreasing, obtains ox bone protein liquid.
2) the Yak Bone Protein liquid that will 1) obtain, temperature are adjusted to 70 DEG C, using supersonic generator through ultrasonic wave (frequency
It is primary using the pre- homogenization of the homogenizer of 20MPa for 70kH) processing 20 minutes, then under conditions of pressure 220Mpa
Super-voltage micro jet is carried out to handle 2 times.
3) adjusting protein content in Yak Bone Protein liquid is 9%, according to the weight of protein by weight 1.0% in Yak Bone Protein liquid
Measure percentage compound protease be added and carry out stepwise discretization, the first step using 0.6% compound protease one (alkali protease, in
Property protease and trypsase composition, mass ratio between them be 1:1:1), enzyme digestion reaction 1.5h is carried out under the conditions of 50 DEG C；
Then carrying out second step, (papain and flavor protease form, the quality between them using 0.4% compound protease two
Than enzyme digestion reaction 1.0h being carried out under the conditions of 50 DEG C for 1:2)；15 minutes are kept the temperature at 95 DEG C, is cooled to room temperature, enzymatic hydrolysis is added
The active carbon of liquid weight 0.2% is separated in enzymolysis liquid by diatomite, and separating liquid is collected；
It 4) is the ceramic membrane ultrafitration of 10000 dalton using aperture by the resulting separating liquid of step 3), it is first that molecular weight is small
Albumen and peptide separation in 10000 come out, then with aperture be 3000 dalton film by molecular weight less than 3000 dalton
Protein peptides are separated.
5) take protein peptides liquid of the molecular weight less than 3000, using Sephadex G-15 gel separate, eluent be go from
Sub- water, eluting peak are detected at 280nm, collect the 1st eluting peak；By being concentrated, being freeze-dried, obtain by gel point
From ox bone protein peptides；1 separation is carried out to the ox bone protein peptides with RP-HPLC reversed-phase high performance liquid chromatography again, reverse phase is high
The concrete operations of effect liquid phase chromatogram separation are as follows: 5~10mL/min of flow rate of mobile phase；Detection wavelength 220nm；Elution program are as follows: stream
Dynamic phase A is pure water, and Mobile phase B is the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；The volume of 0~5min Mobile phase B point
Number is 0%, and the volume fraction of 5~40min Mobile phase B is from 0% to 35%, and the volume fraction of 40~45min Mobile phase B is from 35%
To 95%, 45~50min Mobile phase B takes the 12-14 points of peptide solutions collected from 95% to 0%；
6) peptide solution for obtaining step (5) is by concentration, freeze-drying, obtains having and relieves fatigue and anti-oxidation function
Bone collagen Gly-His-Lys.It is measured by main component of the LC-MS/MS to bone collagen peptide, amino acid sequence is
Val-Ile-Glu-Ala-Val-Val-Gln-Ser-Val, Asn-Phe-Ile-Lys-Gln-Ser；Tyr-Leu-Glu-Glu-
The content of the peptide of Lys, Ala-Gly-Glu-Val-Leu is 58%.
Embodiment 3
The present embodiment is related to a kind of preparation method with antifatigue and anti-oxidation function Yak Bone Protein peptide, including such as
Lower step:
1) fresh 1000 grams of Yak Bone of freezing are selected, is cleaned with the clean water for meeting sanitary standard for drinking water, after ox bone cleaning
Ox bone is broken into skeletal grain with ox bone pulverizer, the water that 3.0 times of ox bone particle total amount is added handles 3 hours under the conditions of 125 DEG C, so
It is obtained boning the ox bone thermophilic digestion liquid of slag by centrifugal filtration afterwards, is cooled to 30 DEG C, by liquid separation tank degreasing, obtains ox bone egg
White liquor；
2) the ox bone protein liquid that will 1) obtain, temperature are adjusted to 75 DEG C, and using supersonic generator, through ultrasonic wave, (frequency is
60kH) handle 25 minutes, it is primary using the pre- homogenization of the homogenizer of 20MPa, then under conditions of pressure 160Mpa into
Row super-voltage micro jet is handled 2 times.
3) adjusting protein content in ox bone protein liquid is 9%, according to the weight hundred of protein by weight 1.2% in ox bone protein liquid
Divide and carry out stepwise discretization than compound protease is added, the first step utilizes 0.8% compound protease one (alkali protease, neutrality egg
White enzyme and trypsase composition, the mass ratio between them are 1:1:1), enzyme digestion reaction 1.0h is carried out under the conditions of 60 DEG C；Then
Carrying out second step, (papain and flavor protease form, and the mass ratio between them is using 0.4% compound protease two
1:1), enzyme digestion reaction 2.0h is carried out under the conditions of 50 DEG C；20 minutes are kept the temperature at 90 DEG C, is cooled to room temperature, enzymolysis liquid weight is added
The active carbon of amount 0.2% is separated in enzymolysis liquid by diatomite, and separating liquid is collected；
4) step (3) resulting separating liquid is handled by two step hyperfiltration process, is 10000 dalton using aperture
Ceramic membrane ultrafitration, first the albumen by molecular weight less than 10000 and peptide separation come out, then with aperture be 3000 dalton film
Protein peptides by molecular weight less than 3000 dalton are separated.
5) taking molecular weight is the protein peptides liquid less than 3000, is separated using Sephadex G-15 gel, and eluent is to go
Ionized water, eluting peak are detected at 280nm, collect the 1st eluting peak；By being concentrated, being freeze-dried, obtain by gel
Isolated ox bone protein peptides；It is separated again with RP-HPLC reversed-phase high performance liquid chromatography, the tool of reversed-phase high performance liquid chromatography separation
Gymnastics conduct: 5~10mL/min of flow rate of mobile phase；Detection wavelength 220nm；Elution program are as follows: mobile phase A is pure water, Mobile phase B
For the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；The volume fraction of 0~5min Mobile phase B is 0%, 5~40min stream
The volume fraction of dynamic phase B is from 0% to 35%, and the volume fraction of 40~45min Mobile phase B is from 35% to 95%, 45~50min stream
Dynamic phase B takes the 12-14 points of peptide solutions collected from 95% to 0%.
6) peptide solution for obtaining step (5) is by concentration, freeze-drying, obtains having and relieves fatigue and anti-oxidation function
Bone collagen Gly-His-Lys.It is measured by main component of the LC-MS/MS to bone collagen peptide, amino acid sequence is
Val-Ile-Glu-Ala-Val-Val-Gln-Ser-Val, Asn-Phe-Ile-Lys-Gln-Ser；Tyr-Leu-Glu-Glu-
The content of the peptide of Lys, Ala-Gly-Glu-Val-Leu is 59%.
Experimental example 1
This experimental example is related to the measurement test of Yak Bone Protein peptide delay fatigue function
Test specimen: Yak Bone egg prepared by sample 1,2,3 respectively embodiment 1, embodiment 2,3 step of embodiment (6)
White peptide, sample 4 are that the Yak Bone Protein liquid of 1 step of embodiment (1) passes through the Yak Bone Protein powder that freeze-drying reaches.
It carries out as follows:
1.1 experimental animal feedings and grouping
200 cleaning grade Kunming mouses, in clean Animal Lab. adaptive feeding one week, every 8~10 cages divided
Cage raising, keeps padding dried sanitary, changes clothes every three days primary.It sweeps on time daily, carries out the work such as sterilization, guarantee clean
Net Animal Lab. neat and tidy.25 ± 2 DEG C of room temperature, relative humidity 50 ± 5%, optical illumination inner room is divided into 12h, with the feeding of mouse quasi-
Expect conventinal breeding, ad lib and drinking-water, records a mouse weight weekly.After one week laundering period, 200 mouse with
Machine is divided into 5 groups, i.e. blank group, 1 group of sample, 2 groups of sample, 3 groups of sample, 4 groups of sample.The physiology salt of naive mice stomach-filling equivalent
Water, other 4 groups of dosage are set as 130mg/kg/d, and stomach-filling during every morning 9:00-10:30 after stomach-filling 30 days, detects tested
The anti-fatigue effect of sample,
1.1.1 swimming with a load attached to the body is tested
After last is to sample 30min, 10 mouse are taken at random from each group, after the sheet lead that its root of the tail portion is wound to 5% weight,
It is placed in swimming trunk went swimming.The depth of water is no less than 30cm, 25 ± 1 DEG C of water temperature, record mouse from swimming to the time of death,
That is the mice burden swimming time.
1.1.2 hepatic glycogen, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase
(GSH-Px) it measures
After last is to sample 30min, takes 10 mouse to pull out eyeball at random from each group and adopt whole blood, by the blood being collected into
3000r/min is centrifuged 10min, takes serum spare, is measured according to CAT, SOD and GSH-Px testing cassete.After the completion of blood sampling, stand
Mouse is dissected, takes liver to be blotted after physiological saline rinses with filter paper, accurately weighs liver 100mg, detected according to hepatic glycogen
Kit explanation is measured.
1.1.3 full blood lactic measures
10 mouse are taken in each group at random, last after sample 30min to taking a blood sample, then in the water that temperature is 25 ± 1 DEG C not
Stop after swimming with a load attached to the body 10min, with capillary from intraocularly blood sampling is adjoined, stops before measurement swimming, after swimming 10min, after swimming respectively
Cease the full blood lactic content of 20min.
The influence (g) of 1 Yak Bone Protein peptide of table and control group to mouse weight
Influence of the 2 Yak Bone Protein peptide of table to mouse anti-reflecting fatigue ability
As can be seen from Table 1 and Table 2, Yak Bone Protein peptide is to the no significant impact of the growth of mouse, but Yak Bone egg
White peptide can extend the swimming time of mouse, can improve mouse energetic supersession system, promote the synthesis of hepatic glycogen, to store
More energy substances promote the vigor of CAT, SOD and GSH-Px enzyme, delay physical fatigue；To lactic acid water after short time movement
Flat raising has apparent inhibiting effect, and body can be helped to delay the accumulation of lactic acid to a certain extent；After rest 20min,
Lactic acid content has to be declined significantly, and body can be helped to accelerate to remove the accumulation of lactic acid.
Experimental example 2
This experimental example is related to the measurement test of Yak Bone Protein peptide antioxidant activity of the present invention
Test specimen:
Yak Bone Protein peptide prepared by sample 1,2,3 respectively embodiment 1, embodiment 2,3 step of embodiment (6), sample 4
The Yak Bone Protein powder reached for the Yak Bone Protein liquid of 1 step of embodiment (1) by freeze-drying.
It carries out as follows:
(1) scavenging ability of DPPH free radical: taking the antioxidation active peptides 1.5mL of 20 μ g/mL, and 99.5% ethyl alcohol is added
1.5mL and 0.02%DPPH ethanol solution 0.675mL mixing, oscillation mix, and water-bath 30min are protected from light at room temperature, then in 517nm
Lower detection architecture light absorption value.Light absorption value is lower, and the scavenging ability of DPPH free radical of system is stronger.Blank control is that sample is molten
Liquid 1.5mL changes deionized water 1.5mL into.
DPPH free radical scavenging ability %=((blank absorbency-sample light absorption value)/blank absorbency) × 100
(2) reducing power measures: taking the antioxidation active peptides 1mL of 20 μ g/mL, is added 0.2M phosphate buffer (pH 6.6)
The potassium ferricyanide solution 2.5mL of 2.5mL and 1% (mass fraction) are mixed, then in 50 DEG C of heating water bath 20min.It takes out rapid
It is cooling, 10% (mass fraction) trichloroacetic acid (TCA) solution 2.5mL is added, is uniformly mixed, is then centrifuged at 3000g
10min.Supernatant 2.5mL is taken, deionized water 2.5mL and 1% (mass fraction) liquor ferri trichloridi 0.5mL is added, it is sufficiently mixed
It is even, 10min is reacted at room temperature, measures absorbance with 700nm wavelength.Light absorption value indicates at the i.e. available 700nm wavelength of reducing power.
From table 3 it can be seen that Yak Bone Protein peptide of the present invention has preferable oxidation resistance, in the condition of 20 μ g/mL
Under, scavenging ability of DPPH free radical reaches 88% or more, and reducing power reaches 0.87 or more and a kind of preferable anti-oxidant
Peptide.
The antioxidant activity tests result of the Yak Bone Protein peptide of the present invention of table 3
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (8)
1. a kind of with the preparation method relieved fatigue with the Yak Bone Protein peptide of anti-oxidation function, which is characterized in that including such as
Lower step:
1) Yak Bone is subjected to boiling at high temperature, obtains ox bone protein liquid；
2) ultrasound is carried out to the ox bone protein liquid and dynamic super-voltage micro jet pre-processes, change ox bone egg in ox bone protein liquid
White institutional framework reduces the usage amount of enzyme to improve the albumen to the sensibility of enzyme；
3) two steps of pretreated ox bone protein liquid substep are digested with protease, the protein liquid after must digesting；
4) hyperfiltration treatment is carried out to the protein liquid after the enzymatic hydrolysis with the ultrafiltration membrane of 3000 dalton, obtained containing the molten of protein peptides
Liquid；
5) solution containing protein peptides is separated by gel column, then will be passed through using reversed-phase high performance liquid chromatography
Substance is separated in first isolated eluting peak of the gel column, collects isolate to get the Yak Bone Protein
Peptide, and the Yak Bone Protein peptide include content be 55~62%, amino acid sequence Val-Ile-Glu-Ala-Val-
Val-Gln-Ser-Val、Asn-Phe-Ile-Lys-Gln-Ser、Tyr-Leu-Glu-Glu-Lys、Ala-Gly-Glu-Val-
The peptide of Leu；
In the step 3), used enzyme is alkali protease, neutral proteinase and tryptose during the first step digests
First complex enzyme of enzyme；Used enzyme is the second compound of papain and flavor protease during second step digests
Enzyme；
In the step 3), the mass ratio of alkali protease, neutral proteinase and trypsase is 1~2:1:1；Papain
Mass ratio with flavor protease is 1:1~2.
2. the method according to claim 1, wherein the actual conditions digested in the step 3) are as follows:
The content for adjusting albumen in pretreated ox bone protein liquid is 6~10%, during the first step digests, described first
The additive amount of complex enzyme is the 0.4~1.0% of the weight of ox bone albumen dry matter, during second step digests, described second
The additive amount of complex enzyme is the 0.3~0.6% of the weight of ox bone albumen dry matter；
For first step enzymatic hydrolysis to digest 0.5~1.5h under the conditions of 45~60 DEG C, second step enzymatic hydrolysis is enzyme under the conditions of 45~55 DEG C
Solve 1.0~2.0h.
3. the method according to claim 1, wherein in the step 1), before carrying out boiling to the Yak Bone,
It is broken into skeletal grain；
The concrete operations of the boiling are as follows: 1.0~3.0 times of ox bone particle weight of water is incorporated as, under the conditions of 121~131 DEG C
Processing 2~5 hours, obtains filtrate after centrifugal filtration, degreasing obtains ox bone protein liquid.
4. the method according to claim 1, wherein the pretreated actual conditions of ultrasound are, by the ox
The temperature of bone protein liquid is adjusted to 65~75 DEG C, and 15~25min is handled under conditions of frequency is 50~80kH；
The pretreated concrete operations of super-voltage micro jet be first pass through 20MPa the pre- homogenization of homogenizer it is primary, then
Super-voltage micro jet is carried out under conditions of 140~220Mpa of pressure to handle 2~3 times.
5. according to the method described in claim 4, eluting peak exists it is characterized in that, the gel column is Sephadex G-15
280nm is detected；
The concrete operations that reversed-phase high performance liquid chromatography separates in step 5) are as follows: 5~10mL/min of flow rate of mobile phase；Detection wavelength
220nm；
Elution program are as follows: mobile phase A is pure water, and Mobile phase B is the acetonitrile solution of the trifluoroacetic acid of volume fraction 0.1%；
The volume fraction of 0~5min Mobile phase B be 0%, the volume fraction of 5~40min Mobile phase B from 0% to 35%, 40~
The volume fraction of 45min Mobile phase B is from 35% to 95%, and 45~50min Mobile phase B is from 95% to 0%.
6. according to the method described in claim 5, it is characterized in that, collecting 12 during reversed-phase high performance liquid chromatography separates
~14min isolated peptide solution.
7. what any one the method was prepared according to claim 1~6 has the yak relieved fatigue with anti-oxidation function
Bone protein peptide.
8. it is according to claim 7 have relieve fatigue the Yak Bone Protein peptide with anti-oxidation function as food or guarantor
Application in strong product.
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| CN110669813A (en) * | 2019-10-28 | 2020-01-10 | 山西原生肽科技有限公司 | Yak rib small molecule peptide and extraction method thereof |
| CN111635923B (en) * | 2020-06-12 | 2021-11-02 | 福建农林大学 | Eel anti-inflammatory glycopeptide and preparation method thereof |
| CN112741203B (en) * | 2021-01-19 | 2022-09-06 | 西南民族大学 | Preparation method of liver phosphorylated peptide, antioxidant meat product and preparation method |
| CN112877391B (en) * | 2021-02-19 | 2022-09-16 | 安徽国肽生物科技有限公司 | Bovine bone protein glycopeptide with probiotic growth promoting and oxidation resisting effects and preparation method and application thereof |
| CN112956702A (en) * | 2021-03-25 | 2021-06-15 | 江西邦泰绿色生物合成生态产业园发展有限公司 | Compound preparation for increasing bone mineral density |
| CN113087773B (en) * | 2021-04-29 | 2022-02-18 | 安徽国肽生物科技有限公司 | Yak bone peptide with blood sugar reducing and antioxidant functions and preparation method thereof |
| CN113151391B (en) * | 2021-05-18 | 2023-05-05 | 青海国肽生物科技有限公司 | Yak bone collagen peptide for improving intestinal microorganism abundance function and preparation method thereof |
| CN113615783A (en) * | 2021-08-01 | 2021-11-09 | 青海瑞肽生物科技有限公司 | Yak collagen peptide sports drink |
| CN114042146B (en) * | 2021-11-11 | 2024-04-30 | 安徽国肽生物科技有限公司 | Bovine bone peptide composition and application thereof in preparation of medicines for regulating intestinal flora and preventing and treating osteoporosis |
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-
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Non-Patent Citations (3)
| Title |
|---|
| 牦牛骨蛋白的酶解条件研究;李帆 等;《氨基酸和生物资源》;20061230;第28卷(第4期);全文 |
| 猪骨素的酶解及酶解产物的体外抗氧化活性研究;黎曦;《中国优秀硕士学位论文全文数据库（电子期刊） 工程科技I辑》;20110415(第4期);B024-100 |
| 超高压酶解提取猪腿骨胶原蛋白;李红霞 等;《皮革科学与工程》;20160831;第26卷(第4期);全文 |
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