CN108328727B - 一种糖蜜酒精废水脱色菌组的驯化方法 - Google Patents

一种糖蜜酒精废水脱色菌组的驯化方法 Download PDF

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CN108328727B
CN108328727B CN201810082421.1A CN201810082421A CN108328727B CN 108328727 B CN108328727 B CN 108328727B CN 201810082421 A CN201810082421 A CN 201810082421A CN 108328727 B CN108328727 B CN 108328727B
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黄自宇
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Abstract

本发明属于废水脱色微生物处理技术领域,具体涉及一种糖蜜酒精废水脱色菌组的驯化方法。本发明主要采用糖蜜原液+糖蜜酒精废水的方式进行菌种的驯化。糖蜜酒精废水脱色菌组先经过活化,提高微生物的活力;经第一阶段驯化,辅以糖蜜原液,使微生物逐渐适应糖蜜酒精废水环境;再经第二阶段驯化,通过添加鲜鬼针草,为微生物提供微量元素及生长因子,提高微生物生长能力,从而提高微生物适应糖蜜酒精废水适应能力及降解COD及色素的能力,解决了微生物驯化耗时长、脱色难、降解难等问题。本发明具有操作简单、耗时短、CODcr及色素降解率高、成本低等优点。

Description

一种糖蜜酒精废水脱色菌组的驯化方法
【技术领域】
本发明涉及废水脱色微生物处理技术领域,特别涉及一种糖蜜酒精废水脱色菌组的驯化方法。
【背景技术】
糖蜜酒精废水是糖厂酒精车间用糖蜜发酵制取酒精之后排放出的高浓度高色度的有机废水,内含有丰富的蛋白质和其它有机物,也含有较多的N、P、K、Ca、Mg等无机盐和较高浓度的SO4 2-等,如不经过处理直接排出,会严重污染水质、环境,因此,糖蜜酒精废水要经过处理并达标后才能进行排放。但由于糖蜜酒精废水是一类高浓度的有机废水,废水中色素的含量较高,主要由酚类色素、类黑色素、焦糖色素等组成,而色素的主要成分为酚类化合物及氨基氮化合物等,色素的抗氧化特性使得废水脱色成为糖蜜废水治理中的难点。
目前国内处理糖蜜酒精废水的主要工艺路线基本遵行“预处理+生化处理+深度处理”,其中生化处理是糖蜜酒精废水处理工艺的重要阶段。微生物是废水生物处理过程的核心,制约着污水处理的效果。在糖蜜酒精废水处理启动初期或运行受到冲击需要重新恢复运行时,国内普遍采用的方法是向糖蜜酒精废水处理设施中投加市场上现有的专用微生物菌剂,达到废水处理设施正常运行的目的。对于糖蜜酒精废水的处理,投加的菌剂的效果受到以下几方面问题的制约:一、糖蜜酒精废水中含有多种对微生物有毒害作用的物质,投加到污水中的菌种容易被废水中的毒性物质所抑制,为解决此问题往往需要对菌种在投加前进行长时间的驯化;二、糖蜜酒精废水中有大量酚类化合物及氨基氮化合物等难降解有机物,这些物质很难被微生物利用并降解,解决这个问题通常需要工艺的合理设计与菌剂的驯化共同解决;三、与普通生活废水相比,糖蜜酒精废水处理设施的启动时间更长,需要更长时间的驯化。
【发明内容】
鉴于上述内容,有必要提供一种糖蜜酒精废水脱色菌组的驯化方法,该方法操作简单、耗时短、成本低,提高CODcr及色素降解效率,具有实用性。
为达到上述目的,本发明一种糖蜜酒精废水脱色菌组的驯化方法所采用的技术方案如下:
一种糖蜜酒精废水脱色菌组的驯化方法,包括以下步骤:
(1)初步液体培养基的配制:取牛肉膏1g,蛋白胨1g,糖蜜原液70g,FeSO4·4H2O0.03g,用1.4L水溶解,以上培养基用质量分数为10%的碳酸钠调节pH值为6.8-7.5,并平均分成7份分装到锥形瓶中,121℃灭菌15min,待冷却备用;
(2)活化菌群:取黑曲霉、硝化菌、枯草芽孢杆菌、地衣芽孢杆菌、嗜热侧孢霉、醋酸菌、酵母菌各1g放入上述步骤(1)已灭好菌的培养基中,30-45℃摇床培养24-48h;
(3)第一阶段驯化:将CODcr为2500-3000mg/L,色度为1500-2000倍的糖蜜酒精废水分别通入第一组7个模拟上流式厌氧污泥床反应器生长环境的10L一级驯化罐中,并加入1-2L糖蜜原液,调节pH值为6.0-6.5;将上述步骤(2)中活化的菌种分别接入所述一级驯化罐中,厌氧驯化12-24h;
(4)第二阶段驯化:将CODcr为3000-5000mg/L,色度为2000-3000倍的糖蜜酒精废水分别通入第二组7个模拟上流式厌氧污泥床反应器生长环境的20L二级驯化罐中,并加入0.5-1kg的鲜鬼针草,调节pH值为5.0-6.5;将上述步骤(3)中驯化的菌液分别接入所述二级驯化罐中,厌氧驯化12-24h;此驯化后的菌种为糖蜜酒精废水厌氧处理工段的驯化菌种。
进一步的,上述步骤(4)中所述鲜鬼针草剪切成2-3cm长度。
本发明具有以下有益效果:
本发明的一种糖蜜酒精废水脱色菌组的驯化方法主要采用糖蜜原液+糖蜜酒精废水的方式进行菌种的驯化。糖蜜酒精废水脱色菌组先经过活化,提高微生物的活力;经第一阶段驯化,辅以糖蜜原液,使微生物逐渐适应糖蜜酒精废水环境;再经第二阶段驯化,通过添加鲜鬼针草,为微生物提供微量元素及生长因子,提高微生物生长能力,从而提高微生物适应糖蜜酒精废水适应能力及降解COD及色素的能力。本发明具有操作简单、耗时短、成本低的优点。
【附图说明】
图1是本发明一种糖蜜酒精废水脱色菌组的驯化方法实施方式的工艺流程示意图。
为了更好的理解本发明的技术方案,下面结合具体实施方式进行阐述。
【具体实施方式】
结合附图,本发明一种糖蜜酒精废水脱色菌组的驯化方法具体操作描述如下:
实施例1
(1)初步液体培养基的配制:取牛肉膏1g,蛋白胨1g,糖蜜原液70g,FeSO4·4H2O0.03g,用1.4L水溶解,以上培养基用质量分数为10%的碳酸钠调节pH值为6.8-7.5,并平均分成7份分装到锥形瓶中,121℃灭菌15min,待冷却备用;
(2)活化菌群:取黑曲霉、硝化菌、枯草芽孢杆菌、地衣芽孢杆菌、嗜热侧孢霉、醋酸菌、酵母菌各1g放入上述步骤(1)已灭好菌的培养基中,30-45℃摇床培养24-48h;
(3)第一阶段驯化:将CODcr为2500mg/L,色度为1500倍的糖蜜酒精废水分别通入第一组7个模拟上流式厌氧污泥床反应器生长环境的10L一级驯化罐中,并加入1-2L糖蜜原液,调节pH值为6.0-6.5;将上述步骤(2)中活化的菌种分别接入所述一级驯化罐中,厌氧驯化12-24h;
(4)第二阶段驯化:将CODcr为3000mg/L,色度为2000倍的糖蜜酒精废水分别通入第二组7个模拟上流式厌氧污泥床反应器生长环境的20L二级驯化罐中,并加入0.5-1kg的鲜鬼针草,调节pH值为5.0-6.5;将上述步骤(3)中驯化的菌液分别接入所述二级驯化罐中,厌氧驯化12-24h;此驯化后的菌种为糖蜜酒精废水厌氧处理工段的驯化菌种。
其中,上述步骤(4)中所述鲜鬼针草剪切成2-3cm长度。
经检测,本实施例驯化后厌氧工段出水的CODcr去除率为90%,色度去除率为79%。
实施例2
实施例2与实施例1的区别仅仅在于:第一阶段驯化时,糖蜜酒精废水的CODcr为3000mg/L,色度为2000倍;第二阶段驯化时,糖蜜酒精废水的CODcr为4000mg/L,色度为2700倍。
经检测,本实施例驯化后厌氧工段出水的CODcr去除率为89%,色度去除率为75%。
实施例3
实施例3与实施例1的区别仅仅在于:第一阶段驯化时,糖蜜酒精废水的CODcr为3000mg/L,色度为2000倍;第二阶段驯化时,糖蜜酒精废水的CODcr为5000mg/L,色度为3000倍。
经检测,本实施例驯化后厌氧工段出水的CODcr去除率为87%,色度去除率为72%。
上述说明是针对本发明较佳可行实施例的详细说明,但实施例并非用以限定本发明的专利申请范围,凡本发明所提示的技术精神下所完成的同等变化或修饰变更,均应属于本发明所涵盖专利范围。

Claims (2)

1.一种糖蜜酒精废水脱色菌组的驯化方法,其特征在于,包括以下步骤:
(1)初步液体培养基的配制:取牛肉膏1g,蛋白胨1g,糖蜜原液70g,FeSO4·4H2O 0.03g,用1.4L水溶解,以上培养基用质量分数为10%的碳酸钠调节pH值为6.8-7.5,并平均分成7份分装到锥形瓶中,121℃灭菌15min,待冷却备用;
(2)活化菌群:取黑曲霉、硝化菌、枯草芽孢杆菌、地衣芽孢杆菌、嗜热侧孢霉、醋酸菌、酵母菌各1g放入上述步骤(1)已灭好菌的培养基中,30-45℃摇床培养24-48h;
(3)第一阶段驯化:将CODcr为2500-3000mg/L,色度为1500-2000倍的糖蜜酒精废水分别通入第一组7个模拟上流式厌氧污泥床反应器生长环境的10L一级驯化罐中,并加入1-2L糖蜜原液,调节pH值为6.0-6.5;将上述步骤(2)中活化的菌种分别接入所述一级驯化罐中,厌氧驯化12-24h;
(4)第二阶段驯化:将CODcr为3000-5000mg/L,色度为2000-3000倍的糖蜜酒精废水分别通入第二组7个模拟上流式厌氧污泥床反应器生长环境的20L二级驯化罐中,并加入0.5-1kg的鲜鬼针草,调节pH值为5.0-6.5;将上述步骤(3)中驯化的菌液分别接入所述二级驯化罐中,厌氧驯化12-24h;此驯化后的菌种为糖蜜酒精废水厌氧处理工段的驯化菌种。
2.如权利要求1所述的一种糖蜜酒精废水脱色菌组的驯化方法,其特征在于步骤(4)中所述鲜鬼针草剪切成2-3cm长度。
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