CN108324606B - Pitaya stem extract and preparation method and application thereof - Google Patents
Pitaya stem extract and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a dragon fruit stem extract and a preparation method and application thereof, which comprises the following steps of (a) cleaning dragon fruit stems, removing thorns and cutting into sections, crushing by a colloid mill, adding deionized water, adding diluted pectinase, and stirring and extracting; (b) inactivating enzyme, cooling, coarse filtering, and fine filtering; (c) decolorizing, cooling, filtering, sterilizing and preserving filtrate to obtain the dragon fruit stem extract provided by the invention. Compared with the prior art, the method for preparing the dragon fruit stem extract has the advantages of simple and convenient extraction and separation operation, high extraction rate of active ingredients and higher purity of the finally prepared product. The dragon fruit stem extract has multiple effects of resisting inflammation, resisting bacteria, moistening skin, promoting wound healing, enhancing capillary vessel circulation and the like. Test tests show that the extract prepared by the method can be used as a moisturizing anti-allergy agent and widely applied to preparation of cosmetics, especially to preparation of cosmetics with moisturizing and anti-allergy effects.
Description
Technical Field
The invention relates to a plant extract, and in particular relates to a dragon fruit stem extract. The invention also relates to a preparation method of the dragon fruit stem extract and application of the dragon fruit stem extract in cosmetics.
Background
Allergy is also known as allergy and is the immune response produced by people with allergic constitution after they have been exposed to allergens. Skin allergy is one of the common skin problems, and in recent years, allergic patients have been increasing sharply with changes in dietary structure, air pollution, and increased pressure on life and work. Skin sensitivity has become a social problem affecting human health. According to incomplete statistics, the population of the global self-sensation sensitive skin is more and more, wherein 39% of men and 61% of women are in the population. The complicated causes and repeated attacks of disease are important problems to be solved in the fields of medicine and cosmetics. Most of the currently used antiallergic ingredients have undefined effects and have certain side effects, and the corticosteroid skin drugs are easy to cause skin thinning and pigmentation. Therefore, the application of the common anti-allergy components in cosmetics is limited, and the appearance of natural plant anti-allergy active components fills the gap in the aspect and is favored by the market and consumers.
Pitaya is a plant of the family Cactaceae and genus Gastrodia, and has thick and dark green stem, generally 3 edges, flat edges and wavy edges. The dragon fruit stem contains rich plant polysaccharide, high contents of three elements of potassium, magnesium and calcium, contains various effective components of phytosterol and the like, has the effects of keeping the moisture on the surface of the skin, promoting the metabolism of the skin, inhibiting skin inflammation and the like, and the extract of the dragon fruit stem can be used as a cosmetic raw material to be applied to a formula. Chinese patent 201410786777.5 discloses a pitaya stem moisturizing liquid and a preparation method thereof, wherein the solvent used for extracting the pitaya stem juice extract in the method is an ethanol solution. Ethanol solution is used as solvent for extraction, and most of the obtained alcohol-soluble substances are obtained.
Disclosure of Invention
The invention aims to provide a preparation method of a dragon fruit stem extract with the effects of moisturizing and anti-allergy.
The dragon fruit stem extract provided by the invention is prepared according to the method comprising the following steps.
(a) Cleaning dragon fruit stems, removing thorns, cutting into sections, cleaning the surfaces of the dragon fruit stems with deionized water, adding the deionized water, grinding the dragon fruit stems by a colloid mill, adding an antioxidant and pectinase or cellulase diluted by the deionized water, and stirring the mixture for a certain time at a certain stirring speed and reaction temperature for extraction.
The dragon fruit stem is an oblate quadrangular juicy stem of a dragon fruit, the production place of the dragon fruit stem is Guangxi, and the dragon fruit stem is purchased from Gaofeng agriculture Limited, Qinzhou; the pectinase or cellulase is purchased from Novoxin (China) biotechnology limited, preferably pectinase, and the type of the pectinase is XXL; the dosage of the antioxidant is 0.03 percent of the mass of the dragon fruit stems.
(b) Inactivating enzyme, cooling, coarse filtering, and fine filtering.
(c) Decolorizing, cooling, filtering, sterilizing the filtrate, and preserving to obtain the dragon fruit stem extract.
Wherein activated carbon is used for decoloring; phenoxyethanol liquid or PE9010 is used as a preservative, the PE9010 is purchased from Germany Shumei company, and the dosage of the preservative is 0.8 percent of the quality of the dragon fruit stem extract.
Pectin is a high molecular weight polysaccharide present in the primary cell wall and intercellular substance that acts to "bind" cells, and when degraded, results in cell separation. The pectinase can break the cell wall of the plant cell, so that effective components in the cell, such as dragon fruit polysaccharide and the like, can be dissolved out more quickly, and the extraction rate is improved; it also has clarifying effect, and can reduce viscosity to make the extract clear and bright. The cellulase is a general name of a group of enzyme systems for degrading cellulose, the main component of the plant cell wall is cellulose, and the cell wall is easier to damage and the effective components are easier to dissolve out through the enzymolysis of the cellulose. When the ethanol solution is used as a solvent for extraction, the dissolution rate of components such as polysaccharide, protein and the like is low, and the extract mainly contains alcohol-soluble substances. Through moisturizing and anti-allergic experiments, the water-soluble substances are verified as main functional components, so that after the dragon fruit stems are juiced, pectinase or cellulase is added for enzymolysis, and the extraction rate of the dragon fruit stems and the dissolution rate of effective components can be greatly improved. And the content of the effective components is increased to produce better anti-allergic moisturizing effect.
Preferably, the mass ratio of the dragon fruit stem to the deionized water in the step (a) is 1:6-20, the antioxidant is D-sodium ascorbate, the pectinase or cellulase and the deionized water are diluted according to the mass ratio of 1:50-80, the dosage of the pectinase or cellulase is 0.06-0.15% of the mass of the dragon fruit stem, the stirring speed is 50-100 r/min, the reaction temperature is 40-60 ℃, and the reaction time is 60-120 min.
Preferably, the enzyme deactivation condition in the step (b) is that the enzyme deactivation temperature is 70-100 ℃, and the enzyme deactivation time is 15-30 min; the final temperature of the cooling step is 20-50 ℃; the gauze used for coarse filtration has 60-200 meshes; the aperture of the filter plate used for fine filtration is 1-10 μm.
Preferably, the decoloring condition in the step (c) is that the reaction temperature is 70-90 ℃, the reaction time is 20-60 min, and the amount of the added active carbon is 0.5-2% of the mass of the filtered dragon fruit stem extract obtained in the step (b); the final temperature of the cooling step is 20-50 ℃; the aperture of the filter plate used for filtering is 0.1-1 μm, the sterilization temperature is 85-95 ℃, and the sterilization time is 20-40 min.
Preferably, the mass ratio of the dragon fruit stems to the deionized water in the step (a) is 1:10, the antioxidant is D-sodium ascorbate, the pectinase or cellulase and the deionized water are diluted according to the mass ratio of 1:60, the dosage of the pectinase or cellulase is 0.1% of the mass of the dragon fruit stems, the stirring speed is 60 r/min, the reaction temperature is 50 ℃, and the reaction time is 90 min.
Preferably, the enzyme deactivation condition in the step (b) is that the enzyme deactivation temperature is 85 ℃ and the enzyme deactivation time is 20 min; the final temperature of the cooling step is 30 ℃; the gauze used for coarse filtration has 200 meshes; the aperture of the filter plate used for fine filtration is 10 mu m.
Preferably, the decoloring condition in the step (c) is that the reaction temperature is 80 ℃, the reaction time is 30 min, and the amount of the added active carbon is 1.0 percent of the mass of the filtered dragon fruit stem extract obtained in the step (b); the final temperature of the cooling step is 30 ℃; the aperture of the filter plate used for filtering is 0.2-0.45 μm, the sterilization condition is that the sterilization temperature is 90 ℃, and the sterilization time is 30 min.
The invention also aims to provide the dragon fruit stem extract prepared by the preparation method of the dragon fruit stem extract.
Preferably, the effective components in the dragon fruit stem extract comprise anthocyanin, vitamin C, phytosterol, plant polysaccharide, plant albumin and water-soluble dietary fiber. The anthocyanin is a powerful antioxidant, can remove excessive free radicals, protects cells from being damaged by the free radicals, inhibits inflammation and allergy, can improve the smoothness of the skin and beautify the skin; the vitamin C has the effects of whitening skin, preventing black spots, increasing collagen and adjusting skin texture; the phytosterol has high permeability to skin, can effectively keep the moisture on the surface of the skin, promote the metabolism of the skin and inhibit the inflammation of the skin, simultaneously has an antioxidant function, and can prevent erythema solare and skin aging; the molecular structure of the plant polysaccharide has the characteristics of water locking and water absorption, and can better moisturize the skin and improve the barrier function of the skin; the plant albumin has certain anti-oxidation, anti-free radical and anti-aging effects, can relieve allergic symptoms and enhance the barrier function of skin, and the effective components have synergistic effect to endow the pitaya stem extract with better anti-allergy and moisturizing effects. More preferably, the content of the anthocyanin is 0.40-0.60 mg/mL, the content of the vitamin C is 0.08-0.20 mg/mL, the content of the phytosterol is 0.06-0.16 mg/mL, and the content of the plant polysaccharide is 2.5-4.0 mg/mL.
The invention also aims to provide the application of the dragon fruit stem extract in preparing cosmetics.
Preferably, the application of the dragon fruit stem extract in preparing cosmetics with the effects of moisturizing and anti-allergy is provided.
Compared with the prior art, the method for preparing the dragon fruit stem extract has the advantages of simple and convenient extraction and separation operation, high extraction rate of effective active ingredients, higher purity of the finally prepared product and great improvement on the product quality. The pitaya stem extract has multiple effects of resisting inflammation, resisting bacteria, moistening skin, whitening and moisturizing, promoting wound healing, enhancing capillary circulation and the like. Test tests show that the extract prepared by the preparation method provided by the invention can be used as a moisturizing anti-allergy agent, and can be widely applied to preparation of cosmetics, especially to preparation of cosmetics with moisturizing and anti-allergy effects.
In the prior art, ethanol is used as a solvent for extraction, most of the obtained alcohol-soluble substances are greatly different from the effective components in the extract obtained by the invention. The extraction rate of the dragon fruit stems and the dissolution rate of the effective components are greatly improved through the enzymolysis of pectinase or cellulase, and the moisture-preserving and anti-allergy effects are more remarkable due to the improvement of the content of the effective components.
Drawings
Fig. 1 is a flow chart of a production process of the dragon fruit stem extract provided by the invention.
Fig. 2 is a hyaluronidase activity inhibition experiment of the pitaya stem extract provided by the invention.
Fig. 3 is a human skin allergy test of the dragon fruit stem extract provided by the invention.
Fig. 4 is a graph showing the results of a test on the rate of change of the moisture content of a human body by using the dragon fruit stem extract provided by the invention.
Fig. 5 is a graph showing the results of a human body moisture loss rate test of the dragon fruit stem extract provided by the invention.
Detailed Description
The invention will now be further described with reference to specific examples.
Example 1: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 6 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, and adding cellulase accounting for 0.06 percent of the weight of the dragon fruit stems, wherein the cellulase and deionized water are diluted according to the mass ratio of 1: 50; extracting at 40 deg.C and 50 r/min for 120 min; (b) heating to 70 deg.C, stirring for 30 min, and sterilizing; stopping heating, cooling to 20 deg.C with water circulation, and coarse-filtering with 60 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 1 mu m; (c) adding 0.5% of activated carbon in the amount of the filtered extract of dragon fruit stem, and stirring at 70 deg.C for 20 min; stopping heating, cooling water to 20 deg.C, and filtering with filter plate with aperture of 0.1 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 4.7-6.2, the solid content is 0.55-1.1%, and the conductivity is 550-1010 mus/cm2(ii) a Then preserving the heat at 85 ℃ for 20 min, and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 2: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 20 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, adding pectinase accounting for 0.15 percent of the weight of the dragon fruit stems, and diluting the pectinase and deionized water according to the mass ratio of 1: 60; extracting at 60 deg.C under stirring at 100 r/min for 60 min; (b) heating to 100 deg.C, stirring for 15 min, and sterilizing; stopStopping heating, cooling to 50 deg.C with water circulation, and coarse-filtering with 150 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 8 mu m; (c) adding activated carbon 2.0% of the filtered extract of dragon fruit stem, and stirring at 90 deg.C for 60 min; stopping heating, cooling water to 50 deg.C, and filtering with filter plate with aperture of 1 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 4.6-6.3, the solid content is 0.59-0.98%, and the conductivity is 530-1000 mu s/cm2(ii) a Then keeping the temperature at 95 ℃ for 40 min, and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 3: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 13 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, and adding cellulase accounting for 0.08 percent of the weight of the dragon fruit stems, wherein the cellulase and deionized water are diluted according to the mass ratio of 1: 70; extracting at 45 deg.C and 80 r/min for 80 min; (b) heating to 80 deg.C, stirring for 25 min, and sterilizing; stopping heating, cooling to 40 deg.C with water circulation, and coarse-filtering with 100 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 5 mu m; (c) adding activated carbon 1.5% of the filtered extract of dragon fruit stem, and stirring at 85 deg.C for 40 min; stopping heating, cooling water to 40 deg.C, and filtering with filter plate with aperture of 0.2 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 4.5-6.0, the solid content is 0.6-1.05%, and the conductivity is 500-1000 mu s/cm2(ii) a Then preserving the heat for 35 min at 88 ℃ and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 4: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 10 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, and adding pectinase accounting for 0.1 percent of the weight of the dragon fruit stems, wherein the pectinase and deionized water are diluted according to the mass ratio of 1: 80; extracting under stirring at 50 deg.C and 60 r/min for 90 min; (b) heating to 85 deg.C, stirring for 20 min, and sterilizing; stopping heating, cooling to 30 deg.C with water circulation, and coarse-filtering with 200 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 10 mu m; (c) adding activated carbon 1% of the filtered extract of the stem of the dragon fruit, and stirring at 80 deg.C for 30 min; stopping heating, cooling water to 30 deg.C, and filtering with filter plate with aperture of 0.45 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 4.5-5.8, the solid content is 0.7-1.0%, and the conductivity is 500-1000 mu s/cm2(ii) a Then preserving the heat at 90 ℃ for 30 min, and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 5: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 17 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, and adding cellulase accounting for 0.12 percent of the weight of the dragon fruit stems, wherein the cellulase and deionized water are diluted according to the mass ratio of 1: 65; extracting at 55 deg.C and 90 r/min for 100 min; (b) heating to 90 deg.C, stirring for 18 min, and sterilizing; stopping heating, cooling to 35 deg.C with water circulation, and coarse-filtering with 180 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 7 mu m; (c) adding activated carbon 1.8% of the filtered extract of dragon fruit stem, stirring at 75 deg.CStirring for 50 min; stopping heating, cooling water to 35 deg.C, and filtering with filter plate with aperture of 0.35 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 5.0-6.5, the solid content is 0.65-0.95%, and the conductivity is 520-1000 mu s/cm2(ii) a Then preserving the heat at 92 ℃ for 25 min, and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 6: preparing a dragon fruit stem extract.
(a) Weighing 10 kg of cleaned and thornless dragon fruit stems, cleaning the surfaces of the dragon fruit stems with deionized water, placing the dragon fruit stems in a colloid mill, slightly adding deionized water into the colloid mill, grinding, and adding deionized water with the mass ratio of 9 to the dragon fruit stems; adding D-sodium ascorbate accounting for 0.03 percent of the weight of the dragon fruit stems, and adding pectinase accounting for 0.12 percent of the weight of the dragon fruit stems, wherein the pectinase and deionized water are diluted according to the mass ratio of 1: 55; extracting at 53 deg.C under stirring at 70 r/min for 70 min; (b) heating to 75 deg.C, stirring for 27 min, and sterilizing; stopping heating, cooling to 25 deg.C with water circulation, and coarse-filtering with 80 mesh gauze; taking the filtrate, and finely filtering the filtrate by using a filter plate with the aperture of 4 mu m; (c) adding 0.8% of activated carbon in the amount of the filtered extract of the dragon fruit stem, and stirring at 82 deg.C for 35 min; stopping heating, cooling water to 25 deg.C, and filtering with filter plate with aperture of 0.3 μm; and (3) measuring the pH value, the solid content and the conductivity, wherein the measurement result is as follows: the pH value is 4.8-6.4, the solid content is 0.62-0.99%, and the conductivity is 500-1000 mu s/cm2(ii) a Then preserving heat at 87 ℃ for 28 min, and sterilizing; cooling to room temperature in water bath, and adding phenoxyethanol liquid antiseptic with a mass of 0.8% of that of the dragon fruit stem extract to obtain the final dragon fruit stem extract.
Example 7: and (3) measuring the effective components of the dragon fruit stem extract.
Taking the dragon fruit stem extract prepared in the example 4 as an example, the effective components of the dragon fruit stem extract are measured, and the measurement results are as follows: determining the anthocyanin content in the dragon fruit stem extract prepared in the example 4 to be 0.52 mg/mL by adopting an iron salt catalysis colorimetric method; determining the content of vitamin C in the dragon fruit stem extract prepared in the example 4 to be 0.186 mg/mL by an iodometry method; determining the content of phytosterol in the dragon fruit stem extract prepared in the example 4 to be 0.12 mg/mL by using a Liebermann-Burchard color development method; the content of polysaccharide in the dragon fruit stem extract prepared in example 4 was measured to be 3.54 mg/mL by the phenol-sulfuric acid method.
Example 8: experiment for inhibiting hyaluronidase activity.
Research shows that hyaluronidase is closely related to inflammation and allergy, and can carry out enzymolysis on hyaluronic acid in vivo, so that hyaluronic acid becomes an acid stimulus with low molecular weight, and an organism is induced to generate sensitive symptoms. The method adopts a hyaluronidase in-vitro inhibition experiment to determine the inhibition effect of the dragon fruit stem extract on the hyaluronidase activity, and the experimental concentrations respectively adopt 1.0%, 2.0%, 5.0%, 8.0% and 10.0% (mass percentage of the dragon fruit stem extract in the system).
Preparation of solutions required for experiments:
acetic acid buffer solution: weighing 1.155 mL of glacial acetic acid, diluting to 100 mL, and uniformly mixing, wherein 4.8 mL is used as A solution; weighing 2.72 g of sodium acetate crystals, adding water to dissolve the crystals until the volume is 100 mL, and uniformly mixing, wherein 45.2 mL is used as a B solution; a, B solution was mixed, and the volume was adjusted to 100 mL with water and mixed well.
Hyaluronidase solution: 10 mg hyaluronidase was weighed into a beaker and 4 mL of acetic acid buffer was added.
Sodium hyaluronate solution: 5 mg of sodium hyaluronate was weighed into a beaker and 10 mL of acetic acid buffer solution was added.
Ellichi reagent (Ehrlich reagent): 0.8 g of p-dimethylaminobenzaldehyde is weighed out and dissolved in 15 mL of concentrated hydrochloric acid and 15 mL of absolute ethanol.
Acetylacetone solution: 3.5 mL of acetylacetone was dissolved in 50 mL of 1.0 mol/L sodium carbonate solution, and the solution was prepared before use.
The experimental steps are as follows:
taking 0.25 mmol/L CaCl20.1 mL of the solution and 0.5 mL of the hyaluronidase solution are cultured for 20 min at 37 ℃ in a heat preservation way; different concentrations of example 4 were added0.5 mL of the prepared dragon fruit stem extract is continuously cultured for 20 min at 37 ℃ in a heat preservation way; adding 0.5 mL sodium hyaluronate solution, keeping the temperature at 37 ℃ for 30 min, and standing at normal temperature for 5 min; adding 0.1 mL of 0.4 mol/L NaOH solution and 0.5 mL of acetylacetone solution, heating in a boiling water bath for 15 min, and immediately cooling with ice water for 5 min; adding 1.0 mL of Ellisib reagent, diluting with 3.0 mL of anhydrous ethanol, standing for developing for 20 min, and measuring the absorbance value with a spectrophotometer.
The calculation formula of the sample on the hyaluronidase inhibition rate is as follows:
hyaluronidase inhibition (%) =
Wherein A1 is the light absorption value of a reference solution, and deionized water is used for replacing the sample obtained in the example 4; a2 is the light absorption value of a reference blank solution, deionized water is used for replacing the sample obtained in the example 4, and acetic acid buffer solution is used for replacing hyaluronidase and sodium hyaluronate solution; b1 is the absorbance value of the sample solution; b2 is the absorbance value of the blank sample solution, and the hyaluronidase and sodium hyaluronate solution are replaced by acetic acid buffer solution.
In the experiment, the wavelength scanning of the sample is firstly carried out in the range of 450-700 nm, the maximum absorption wavelength is determined, and the measurement is carried out at the maximum absorption wavelength.
The hyaluronidase inhibition results of the dragon fruit stem extract obtained in example 4 are shown in fig. 2. As shown in fig. 2, the inhibitory effect on hyaluronidase activity was gradually increased as the concentration of the dragon fruit stem extract was increased. The dragon fruit stem extract obtained in example 4 can effectively inhibit the activity of hyaluronidase, thereby playing a good anti-allergy role.
Example 9: and (3) human body sensitivity test.
Selecting 20 volunteers, half each male and half female, before experiment, cleaning the left and right front arms of the volunteers with clear water, sitting still in the environment with temperature of 20-22 deg.C and relative humidity of 50-60%, and performing skin sensitivity experiment without applying any cosmetic to the subject.
Applying the above paste to the left forearm of the subjectThe dragon fruit stem extract prepared in example 1 was applied once every half hour for 6 times, and the application area was 3X 3cm2The right forearm was left without any product as a blank. Then, the left and right front arms of the subject were coated with a sodium dodecyl sulfate solution (SDS) containing 5% by mass, respectively. The responses of the arms of the subjects were observed, and the statistical results of sensitization rate are shown in fig. 3. As can be seen from the figure, when the dragon fruit extract prepared in example 4 was applied and then the stimulant was applied, the tingling and swelling of the left forearm were not obvious, and the sensitization rate was greatly reduced. The right forearm serving as a blank control has strong stimulation response to the stimulus, the phenomenon of redness and swelling in the skin after the stimulus is applied for 0.5 h is obvious, the tingling sensation is generated, and the sensitization rate of the blank control is high. It is demonstrated that the dragon fruit stem extract prepared in the above example 4 has a good anti-allergy effect.
Example 10: human body moisturizing experiment.
The human body moisturizing experiment comprises a test group consisting of specific experimental groups, and tests the changes of skin moisture and moisture loss before and after the test subject uses the cosmetics (and the cosmetic active ingredients) so as to determine the moisturizing effect of the cosmetics (or the active ingredients).
The experimental method comprises the following steps: 15 male and female volunteers of 15 years to 60 years of age were selected.
(1) Selecting the left arm and the right arm of the subject to be marked in a circulating mode sequentially: a test area sample group and a blank control area;
(2) the technician uses a skin moisture tester Corneometer CM825 and a water loss tester Tewameter TM300 to measure the values, and the average value is recorded as a blank value.
(3) Smearing the sample to be tested on the experimental part which is cleaned by the test subject with clear water.
(4) The subjects measured 5 times after one hour, two hours, and four hours of continuous application of the cosmetics by a technician using a skin moisture meter CM825 and a water loss through skin tester Tewameter TM300, and averaged.
(5) And counting the measured values, and analyzing the skin moisture content and the moisture loss change rule.
The average moisture content of the skin surface of the subject and the percentage of average moisture loss as a function of time are shown in figures 4 and 5. The results show that the dragon fruit stem extract prepared in the above example 4 has a good long-lasting moisturizing effect. It also shows good properties for enhancing the barrier function of the skin.
Moisture retention and anti-allergy experiments prove that the water-soluble substances are main effective components with the moisture retention and anti-allergy effects, so that the extraction method is high in extraction rate and high in content of the moisture retention and anti-allergy effective components.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (7)
1. A preparation method of a dragon fruit stem extract applied to cosmetics is characterized by comprising the following steps,
(a) cleaning dragon fruit stems, removing thorns and cutting into sections, cleaning the surfaces of the dragon fruit stems by deionized water, adding the deionized water, grinding the dragon fruit stems by a colloid mill, adding an antioxidant and pectinase or cellulase diluted by the deionized water, and stirring the mixture for a certain time at a certain stirring speed and reaction temperature for extraction;
wherein the dragon fruit stem is flat round quadrangular juicy stem of dragon fruit;
(b) inactivating enzyme, cooling, coarse filtering, and fine filtering;
(c) decolorizing, cooling, filtering, sterilizing the filtrate to obtain dragon fruit stem extract;
the method comprises the following steps of (a) decoloring by using activated carbon, wherein the mass ratio of the dragon fruit stems to deionized water in the step (a) is 1:6-20, the antioxidant is D-sodium ascorbate, the dosage of the antioxidant is 0.03% of the mass of the dragon fruit stems, the pectinase or cellulase and the deionized water are diluted according to the mass ratio of 1:50-80, the dosage of the pectinase or cellulase is 0.06-0.15% of the mass of the dragon fruit stems, the stirring speed is 50-100 r/min, the reaction temperature is 40-60 ℃, and the reaction time is 60-120 min.
2. The method for preparing a dragon fruit stem extract applied to cosmetics according to claim 1, wherein the enzyme deactivation conditions in the step (b) are an enzyme deactivation temperature of 70-100 ℃ and an enzyme deactivation time of 15-30 min; the final temperature of the cooling step is 20-50 ℃; the gauze used for coarse filtration has 60-200 meshes; the aperture of the filter plate used for fine filtration is 1-10 μm.
3. The method for preparing a dragon fruit stem extract applied to cosmetics according to claim 2, wherein the decoloring condition in the step (c) is a reaction temperature of 70-90 ℃, a reaction time of 20-60 min, and the amount of the added activated carbon is 0.5-2% of the filtered dragon fruit stem extract obtained in the step (b); the final temperature of the cooling step is 20-50 ℃; the aperture of the filter plate used for filtering is 0.1-1 μm, the sterilization temperature is 85-95 ℃, and the sterilization time is 20-40 min.
4. The method for preparing a dragon fruit stem extract applied to cosmetics according to claim 3, wherein the mass ratio of the dragon fruit stem to the deionized water in the step (a) is 1:10, the antioxidant is D-sodium ascorbate, the dosage of the antioxidant is 0.03% of the mass of the dragon fruit stem, the pectinase or cellulase and the deionized water are diluted according to the mass ratio of 1:60, the dosage of the pectinase or cellulase is 0.1% of the mass of the dragon fruit stem, the stirring speed is 60 r/min, the reaction temperature is 50 ℃, and the reaction time is 90 min.
5. The method for preparing a dragon fruit stem extract applied to cosmetics according to claim 4, wherein the enzyme deactivation conditions in the step (b) are an enzyme deactivation temperature of 85 ℃ and an enzyme deactivation time of 20 min; the final temperature of the cooling step is 30 ℃; the gauze used for coarse filtration has 200 meshes; the aperture of the filter plate used for fine filtration is 10 mu m.
6. The method for preparing a dragon fruit stem extract applied to cosmetics according to claim 5, wherein the decoloring condition in the step (c) is a reaction temperature of 80 ℃, a reaction time of 30 min, and the amount of the added activated carbon is 1.0% of the filtered dragon fruit stem extract obtained in the step (b); the final temperature of the cooling step is 30 ℃; the aperture of the filter plate used for filtering is 0.2-0.45 μm, the sterilization condition is that the sterilization temperature is 90 ℃, and the sterilization time is 30 min.
7. The dragon fruit stem extract applied to the cosmetics is prepared by the preparation method of the dragon fruit stem extract according to any one of claims 1-6, wherein the effective components of the dragon fruit stem extract comprise anthocyanin, vitamin C, phytosterol, plant polysaccharide, plant albumin and water-soluble dietary fiber, wherein the content of the anthocyanin is 0.40-0.60 mg/mL, the content of the vitamin C is 0.08-0.20 mg/mL, the content of the phytosterol is 0.06-0.16 mg/mL, and the content of the plant polysaccharide is 2.5-4.0 mg/mL.
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| NL2029555B1 (en) * | 2021-08-27 | 2023-03-15 | Guangxi Academy Agricultural Sciences | Method for simultaneously extracting phytosterols and polysaccharides from pitaya stems |
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| WO2024150777A1 (en) * | 2023-01-11 | 2024-07-18 | 株式会社ジャパン・オーガニック・イールド | Method for obtaining intracellular aqueous liquid from dragon fruit leaves/stems, and cosmetic, skin medicine, antiseptic and hair growing agent produced using same |
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