CN108315218B - Nucleic acid draws fast and detection device - Google Patents
Nucleic acid draws fast and detection device Download PDFInfo
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- CN108315218B CN108315218B CN201810423227.5A CN201810423227A CN108315218B CN 108315218 B CN108315218 B CN 108315218B CN 201810423227 A CN201810423227 A CN 201810423227A CN 108315218 B CN108315218 B CN 108315218B
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Abstract
The invention discloses a device for quickly extracting and detecting nucleic acid, which comprises a base and a detection device, wherein a first placing groove is formed in one side of the interior of the base, a high-speed motor is arranged on the other side of the interior of the base, a second placing groove is formed in the middle of the top end of the high-speed motor, a first extraction tube is clamped in the middle of the first placing groove, a second extraction tube is clamped in the middle of the first extraction tube, a third extraction tube is clamped in the middle of the second extraction tube, a centrifugal tube is clamped in the middle of the second placing groove, a material pump is bolted in the middle of the top end of the base through a supporting seat, and a liquid inlet device is arranged at one end of the material pump and is arranged right above the centrifugal tube. The invention has reasonable design and convenient use, can carry out fine purification after the initial extraction of nucleic acid by arranging the base and the material pump and arranging the high-speed motor in the base, can effectively improve the extraction speed of nucleic acid, has practical functions and is suitable for wide popularization.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a device for quickly extracting and detecting nucleic acid.
Background
The molecular biology detection technology is a crystal which is a great progress in modern molecular biology and molecular genetics, and is developed on the basis that people increasingly deepen understanding of essential life problems such as gene structure, gene expression, regulation and the like, nucleic acid extraction becomes the most important and basic operation in the molecular biology experiment technology, and the extraction of nucleic acid is often involved in the operation.
Disclosure of Invention
The invention mainly aims to provide a device for quickly extracting and detecting nucleic acid, which can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the technical scheme that:
the utility model provides a nucleic acid draws fast and detection device, includes base and detection device, first groove of placeeing has been seted up to the inside one side of base, the inside opposite side of base is provided with high-speed motor, the second groove of placeeing has been seted up at high-speed motor's top middle part, the middle part joint of first groove of placeeing has first extraction tube, the middle part joint of first extraction tube has the second extraction tube, the middle part joint of second extraction tube has the third extraction tube, the middle part joint of second groove of placeeing has the centrifuging tube, the top middle part of base has been bolted through the supporting seat and has been connect the charge pump, the one end of charge pump is provided with inlet means and sets up directly over the centrifuging tube, the other end of charge pump is provided with out liquid device and sets up directly over the third extraction tube, detection device passes through screw fixed connection in the one side that goes out liquid device, detection device includes test probe and slide rail, the detection probe comprises a slide rail, a liquid outlet device, a detection probe and a liquid outlet device, wherein the slide rail is fixedly connected with the liquid outlet device, the middle part of the slide rail is connected with a slide block in a sliding mode, the middle part of the slide block is provided with a threaded hole, the top end of the detection probe is provided with an external thread which is in screwed connection with the threaded hole, and the detection probe is of.
Further, the bottom end of the third extraction pipe is connected with a nucleic acid filtering membrane in a scarf mode.
Further, the inner wall of the second extraction pipe is embedded with a nucleic acid adsorption membrane.
Further, but first extraction tube, second extraction tube, third extraction tube and centrifuging tube are pull dismantlement structure.
Further, go out the liquid device and include first lift sleeve and drain pipe, the drain pipe is elevation structure for first lift sleeve, the drain pipe runs through in first telescopic middle part that goes up and down, the bottom scarf joint of drain pipe has the shower nozzle, the surperficial department of being close to the shower nozzle of drain pipe is scarf jointed and is had the degree of depth inductor, the degree of depth inductor is semiconductor sensor, the output of degree of depth inductor and the input electric connection of material pump.
Further, the liquid inlet device comprises a second lifting sleeve and a liquid suction pipe, the liquid suction pipe is of a lifting structure relative to the second lifting sleeve, the liquid suction pipe penetrates through the middle of the second lifting sleeve, and a liquid suction cover is screwed at the bottom end of the liquid suction pipe.
Further, the inside roof bolt of base has the electric fan heater, the bilateral inner wall symmetry of base is provided with the ultraviolet ray sterilamp, the bottom middle part scarf joint of base has temperature sensor, the base is hollow structure, and the surface wall in groove and the first groove of placeeing of second is transparent structure.
Further, the use method comprises the following steps:
A. when the device works, a high-speed motor is arranged in the base to rapidly crack cells and initially extract nucleic acid;
B. then the feed pump discharges the nucleic acid solution into a third extraction tube through a liquid outlet device through a liquid inlet device;
C. then the third extraction tube and the second extraction tube are matched with a nucleic acid filtering membrane and a nucleic acid adsorption membrane for use, so that impurities in the nucleic acid can be filtered out, and the nucleic acid can be adsorbed and purified;
D. the nucleic acid adsorbed in the second extraction tube is taken out, and the impurities are placed in the first extraction tube and the third extraction tube, so that the recovery and the cleaning are convenient;
E. in the process of detecting nucleic acid, the head of a detection probe in the detection device is dipped in a certain amount of TMB/H2O2 color development solution, and whether the extraction of nucleic acid is successful or not is detected.
Compared with the prior art, the invention has the following beneficial effects: the invention has reasonable design and convenient use, can realize the rapid lysis of cells by arranging the base and the material pump and arranging the high-speed motor in the base so as to carry out the initial extraction of nucleic acid, then can filter out impurities in the nucleic acid by matching the material pump, the third extraction tube and the second extraction tube, and carry out the adsorption and purification of the nucleic acid, thereby achieving the effect of fine purification, and finally only needing to take up the second extraction tube and take out the nucleic acid adsorbed in the second extraction tube, thereby effectively improving the extraction speed of the nucleic acid, simplifying the whole process, having higher automation degree, arranging the detection probe in the detection device, only needing to dip some TMB/H2O2 color developing solution on the head of the detection probe when the extraction of the nucleic acid is required to be successful, because the TMB/H2O2 color developing solution has the characteristic of blue color development when encountering the nucleic acid, if according to the color development degree of probe, whether still have nucleic acid in the third extraction tube can be known to the naked eye, through being provided with ultraviolet sterilamp and warm braw machine, can realize drawing the regulation of environment, makes it be in aseptic and suitable temperature, and the function is practical, is fit for extensively promoting.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic view of a third extraction tube according to the present invention.
FIG. 3 is a schematic view of a second extraction tube according to the present invention.
Fig. 4 is a schematic structural view of the liquid outlet device of the present invention.
Fig. 5 is a schematic structural diagram of the liquid inlet device of the invention.
FIG. 6 is a schematic structural diagram of the detecting device of the present invention.
In the figure: 1. a third extraction tube; 2. a second extraction tube; 3. a first extraction tube; 4. a base; 5. a liquid outlet device; 6. a liquid inlet device; 7. a material pump; 8. a first placing groove; 9. a high-speed motor; 10. a second placing groove; 11. centrifuging the tube; 12. a detection device; 13. a supporting seat; 14. a warm air blower; 15. an ultraviolet sterilizing lamp; 16. a temperature sensor; 101. a nucleic acid filtration membrane; 201. a nucleic acid-adsorbing membrane; 501. a first lifting sleeve; 502. a liquid outlet pipe; 503. a depth sensor; 504. a spray head; 601. a second lifting sleeve; 602. a pipette; 603. a liquid suction cover; 121. detecting a probe; 122. a slider; 123. a threaded hole; 124. an external thread; 125. a slide rail.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in FIGS. 1-6, a device for rapidly extracting and detecting nucleic acid comprises a base 4 and a detection device 12, wherein a first placing groove 8 is formed in one side inside the base 4, a high-speed motor 9 is arranged on the other side inside the base 4, a second placing groove 10 is formed in the middle of the top end of the high-speed motor 9, a first extraction tube 3 is connected to the middle of the first placing groove 8 in a clamped manner, a second extraction tube 2 is connected to the middle of the first extraction tube 3 in a clamped manner, a third extraction tube 1 is connected to the middle of the second extraction tube 2 in a clamped manner, a centrifugal tube 11 is connected to the middle of the second placing groove 10 in a clamped manner, a material pump 7 is fixedly bolted in the middle of the top end of the base 4 through a support base 13, one end of the material pump 7 is provided with a liquid inlet device 6 and is arranged right above the centrifugal tube 11, the other end of the material pump 7 is provided with a liquid outlet, therefore, the extraction of nucleic acid can be effectively realized, the detection device 12 is fixedly connected to one side of the liquid outlet device 5 through screws, the detection device 12 comprises a detection probe 121 and a slide rail 125, the slide rail 125 is fixedly connected with the liquid outlet device 5, the middle part of the slide rail 125 is slidably connected with a slide block 122, the middle part of the slide block 122 is provided with a threaded hole 123, the top end of the detection probe 121 is provided with an external thread 124, the external thread 124 is screwed with the threaded hole 123, the detection probe 121 is of a detachable structure relative to the external thread 124, the detection of nucleic acid can be realized, and the detection probe 121 can be conveniently maintained and.
Wherein, the bottom end of the third extraction tube 1 is embedded with a nucleic acid filtering membrane 101, and impurities in the nucleic acid solution are filtered out.
Wherein, the inner wall of the second extraction tube 2 is embedded with a nucleic acid adsorption film 201, so that nucleic acid is effectively adsorbed.
Wherein, first extraction tube 3, second extraction tube 2, third extraction tube 1 and centrifuging tube 11 are drawable dismantlement structure, are convenient for take out the nucleic acid in the second extraction tube 2.
Wherein, go out liquid device 5 and include first lift sleeve 501 and drain pipe 502, drain pipe 502 is elevation structure for first lift sleeve 501, drain pipe 502 runs through in the middle part of first lift sleeve 501, the bottom scarf joint of drain pipe 502 has shower nozzle 504, the surface of drain pipe 502 is close to shower nozzle 504 department and has scarf joint degree of depth inductor 503, degree of depth inductor 503 is semiconductor sensor for the degree of depth of sensing purification solution, degree of depth inductor 503's output and charge pump 7's input electric connection realize going out the effect of liquid.
The liquid inlet device 6 comprises a second lifting sleeve 601 and a pipette 602, the pipette 602 is of a lifting structure relative to the second lifting sleeve 601, the pipette 602 penetrates through the middle of the second lifting sleeve 601, and a pipette hood 603 is screwed at the bottom end of the pipette 602 to achieve the effect of sucking nucleic acid solution.
Wherein, the inside roof bolt of base 4 has electric fan heater 14, the both sides inner wall symmetry of base 4 is provided with ultraviolet sterilamp 15, realizes the effect of sterilization, the bottom middle part scarf joint of base 4 has temperature sensor 16, realizes temperature regulation, base 4 is hollow structure, and the table wall of second placer groove 10 and first placer groove 8 is transparent structure, reaches the effect of all-round shining.
It should be noted that, the invention is a nucleic acid rapid extraction and detection device, when in operation, the device is provided with a base 4 and a material pump 7, and a high-speed motor 9 is arranged in the base 4, so as to realize rapid cell lysis, thereby performing initial extraction of nucleic acid, then the material pump 7 discharges a nucleic acid solution into a third extraction tube 1 through a liquid inlet device 5 through a liquid outlet device 6, then the third extraction tube 1 and a second extraction tube 2 are used in cooperation through a nucleic acid filtering membrane 101 and a nucleic acid adsorption membrane 201, so as to filter out impurities in nucleic acid, and perform adsorption and purification of nucleic acid, so as to achieve the effect of fine purification, finally, only the second extraction tube 2 is taken up, the nucleic acid adsorbed in the second extraction tube 2 is taken out, and the impurities are placed in the first extraction tube 3 and the third extraction tube 1, so as to be convenient for recovery and cleaning, and in the detection process of nucleic acid, through being provided with test probe 121 in detection device 12, if need to detect when nucleic acid has or not to draw successfully, only need dip in some TMB H2O2 color development solution at test probe 121's head can, because TMB H2O2 color development solution meets the blue characteristic of nucleic acid development, if according to test probe 121's color development degree, whether the naked eye can know still has nucleic acid in the third extraction tube 1, and be provided with slider 122 and slide rail 125, can be convenient for test probe 121 stretch into and detect in third extraction tube 1.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The utility model provides a quick extraction of nucleic acid and detection device, includes base (4) and detection device (12), its characterized in that: the device is characterized in that a first placing groove (8) is formed in one side inside the base (4), a high-speed motor (9) is arranged on the other side inside the base (4), a second placing groove (10) is formed in the middle of the top end of the high-speed motor (9), a first extraction tube (3) is connected to the middle of the first placing groove (8), a second extraction tube (2) is connected to the middle of the first extraction tube (3), a third extraction tube (1) is connected to the middle of the second extraction tube (2), a centrifugal tube (11) is connected to the middle of the second placing groove (10), a material pump (7) is fixedly bolted to the middle of the top end of the base (4) through a supporting seat (13), one end of the material pump (7) is provided with a liquid inlet device (6) and is arranged over the centrifugal tube (11), and a liquid outlet device (5) is arranged at the other end of the material pump (7) and is arranged over the third extraction tube (1), the detection device (12) is fixedly connected to one side of the liquid outlet device (5) through a screw, the detection device (12) comprises a detection probe (121) and a sliding rail (125), the sliding rail (125) is fixedly connected with the liquid outlet device (5), the middle of the sliding rail (125) is connected with a sliding block (122) in a sliding mode, the middle of the sliding block (122) is provided with a threaded hole (123), the top end of the detection probe (121) is provided with an external thread (124) which is connected with the threaded hole (123) in a screwing mode, and the detection probe (121) is of a detachable structure relative to the external thread (124);
the first extraction tube (3), the second extraction tube (2), the third extraction tube (1) and the centrifuge tube (11) are of drawable and detachable structures;
go out liquid device (5) and include first lift sleeve (501) and drain pipe (502), drain pipe (502) are elevation structure for first lift sleeve (501), drain pipe (502) run through in the middle part of first lift sleeve (501), the bottom scarf joint of drain pipe (502) has shower nozzle (504), the surface of drain pipe (502) is close to shower nozzle (504) department and has scarf joint degree of depth inductor (503), degree of depth inductor (503) are semiconductor sensor, the output of degree of depth inductor (503) and the input electric connection of material pump (7).
2. The apparatus for rapid extraction and detection of nucleic acid as claimed in claim 1, wherein: the bottom end of the third extraction tube (1) is embedded with a nucleic acid filtering membrane (101).
3. The apparatus for rapid extraction and detection of nucleic acid as claimed in claim 1, wherein: the inner wall of the second extraction tube (2) is embedded with a nucleic acid adsorption film (201).
4. The apparatus for rapid extraction and detection of nucleic acid as claimed in claim 1, wherein: the liquid inlet device (6) comprises a second lifting sleeve (601) and a liquid suction pipe (602), wherein the liquid suction pipe (602) is of a lifting structure relative to the second lifting sleeve (601), the liquid suction pipe (602) penetrates through the middle of the second lifting sleeve (601), and the bottom end of the liquid suction pipe (602) is screwed with a liquid suction cover (603).
5. The apparatus for rapid extraction and detection of nucleic acid as claimed in claim 1, wherein: the ceiling bolt of base (4) inside has connect electric fan heater (14), the bilateral inner wall symmetry of base (4) is provided with ultraviolet ray sterilization lamp (15), the bottom middle part of base (4) has inlayed and has connect temperature sensor (16), base (4) are hollow structure, and the table wall that groove (10) and first place groove (8) is placed to the second is transparent structure.
6. The use method of the device for rapidly extracting and detecting nucleic acid according to claim 1, wherein the device comprises: the method comprises the following steps:
A. when the device works, a high-speed motor is arranged in the base to rapidly crack cells and initially extract nucleic acid;
B. then the feed pump discharges the nucleic acid solution into a third extraction tube through a liquid outlet device through a liquid inlet device;
C. then the third extraction tube and the second extraction tube are matched with a nucleic acid filtering membrane and a nucleic acid adsorption membrane for use, so that impurities in the nucleic acid can be filtered out, and the nucleic acid can be adsorbed and purified;
D. the nucleic acid adsorbed in the second extraction tube is taken out, and the impurities are placed in the first extraction tube and the third extraction tube, so that the recovery and the cleaning are convenient;
E. in the process of detecting nucleic acid, the head of a detection probe in the detection device is dipped in a certain amount of TMB/H2O2 color development solution, and whether the extraction of nucleic acid is successful or not is detected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810423227.5A CN108315218B (en) | 2018-05-05 | 2018-05-05 | Nucleic acid draws fast and detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810423227.5A CN108315218B (en) | 2018-05-05 | 2018-05-05 | Nucleic acid draws fast and detection device |
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| CN108315218A CN108315218A (en) | 2018-07-24 |
| CN108315218B true CN108315218B (en) | 2020-09-01 |
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| CN201810423227.5A Active CN108315218B (en) | 2018-05-05 | 2018-05-05 | Nucleic acid draws fast and detection device |
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Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3551293B2 (en) * | 1998-02-02 | 2004-08-04 | 東洋紡績株式会社 | Nucleic acid extraction device |
| EP1391507B1 (en) * | 2001-05-25 | 2012-03-21 | Hitachi, Ltd. | Apparatus for purifying nucleic acid and method of purifying nucleic acid |
| CN102492603B (en) * | 2011-11-22 | 2013-05-01 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN106119101B (en) * | 2013-10-15 | 2018-07-13 | 周国华 | Collect nucleic acid extraction, amplification and detects integrated device |
| CN106404508A (en) * | 2016-11-23 | 2017-02-15 | 杭州杰毅麦特医疗器械有限公司 | Nucleic acid detection pretreatment automation device |
| CN206232713U (en) * | 2016-12-01 | 2017-06-09 | 山东森芃生物科技有限公司 | For micro cast-off cells DNA rapid extraction devices |
| CN107034134A (en) * | 2017-06-08 | 2017-08-11 | 湖南工业大学 | A kind of rotary detection of nucleic acids cartridge |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
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Effective date of registration: 20181228 Address after: 238000 Hefei Vocational and Technical College, Chaohu Economic Development Zone, Anhui Province Applicant after: Hefei Vocationl Technical College Address before: 238000 Hefei Vocational and Technical College, Chaohu Economic Development Zone, Anhui Province Applicant before: Cai Yuhua |
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