CN108314744A - A kind of method of rapid extraction Dendrobium officinale polysaccharide - Google Patents
A kind of method of rapid extraction Dendrobium officinale polysaccharide Download PDFInfo
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The present invention provides a kind of methods of rapid extraction Dendrobium officinale polysaccharide, include the following steps：1) fresh dendrobium candidum stem is mixed with water and carries out historrhexis, obtain dendrobium candidum slurries；2) the dendrobium candidum slurries obtained in step 1) are mixed with the water for being equivalent to raw material 10~20 times of fresh dendrobium candidum stem, 80~100 DEG C of volume and carries out the first polysaccharide elution, obtain the first eluent；3) first eluent is filtered, the first filtrate and the first filter residue are obtained；It includes Dendrobium officinale polysaccharide to be in first filtrate.The method is easy to operate, quick, safe and pollution-free, and recovery rate is high.
Description
Technical field
The invention belongs to technical field of polysaccharide extraction, and in particular to a kind of method of rapid extraction Dendrobium officinale polysaccharide.
Background technology
Dendrobium candidum is orchid family (Orchidaceae) Dendrobium (Dendrobium SW.) plant dendrobium candidum
The drying of (Dendrobium officinaleKimura etMigo) or fresh stem also known as ribbed hedyotis herb, iron bracketplant, iron sheet orchid,
Processing is helically or the habit of spring-like claims Tiepi Fengdou or earrings dendrobium, the habit of segment processing to claim dendrobium candidum.Dendrobium candidum
It is the traditional rare traditional Chinese medicine in China, is the superfine product in the stem of noble dendrobium, civil to be referred to as " help celestial grass ", international medicinal plant circle is called
" medicine circle giant panda "；It is mainly distributed on the ground such as China Zhejiang, Yunnan, Anhui, Fujian, Guangxi, Guangdong, Jiangsu.2010 editions drugs
The legal technical standard of technical supervision ---《Pharmacopoeia of People's Republic of China》, because dendrobium candidum value is significantly larger than other stems of noble dendrobium, it is
Dendrobium candidum is divided as a newly-increased kind from the stem of noble dendrobium list for the first time by the sound development for the dendrobium candidum industry that adequately protects
Come.
Dendrobium candidum has effects that reinforcing stomach reg fluid, nourishing Yin and clearing heat, for pyreticosis Tianjin wound, dry polydipsia, deficiency of stomach-Yin, food
Few retch, abnormal heat does not move back, is fire excess from yin deficiency after being ill, and hectic fever due to yin labor heat, mesh is secretly unknown, muscles and bones impotence.Modern pharmacology and clinical research card
Bright, dendrobium candidum has the effects that antitumor, anti-aging, reduces blood glucose and improve immunity, in the auxiliary treatment of malignant tumour
And chronic pharyngitis, chronic gastritis, diabetes and weakness due to chronic disease immunologic hypofunction etc. are widely used.
Plant chemical ingredient analysis and pharmacology etc. are research shows that bioactive substance is mainly polysaccharide in dendrobium candidum
Compound.Dendrobium officinale polysaccharide is the water-soluble polysaccharide substance extracted from dendrobium candidum, and content is higher,《Chinese Pharmacopoeia》Note
25% must not be less than in terms of anhydrous glucose sugar by carrying.Dendrobium officinale polysaccharide has the works such as antioxygen, immunological regulation, antitumor, hypoglycemic
With.The extracting method of Dendrobium officinale polysaccharide mainly has hot water extraction method, zymohydrolysis extracting method, ultrasonic wave assisted extraction method, microwave auxiliary
Help extraction method, ultrahigh-pressure extraction method etc..Such as：Number of patent application is Chinese patent " a kind of iron sheet of " 201510747301.5 "
The extracting method of dendrobium polysaccharide " and Chinese patent " a kind of ultrasonic wave optimization that number of patent application is " 201510362041.X "
The method for extracting Dendrobium officinale polysaccharide ", is both needed to that processing first is dried to dendrobium candidum, using dry dendrobium candidum as raw material.
Number of patent application is the Chinese patent " a kind of extracting method of Dendrobium officinale polysaccharide " of " 201410010334.7 " although using new
Fresh dendrobium candidum, but organic solvent, alkaline solution are largely used in extraction process, easily pollute the environment.
In currently available technology the extracting method of Dendrobium officinale polysaccharide there are extraction time length, complex steps, pollution environment,
The problems such as being not suitable for large-scale production.
Invention content
In view of this, the purpose of the present invention is to provide a kind of quick, simple, free of contamination extraction Dendrobium officinale polysaccharides
Method.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：A kind of rapid extraction Dendrobium officinale polysaccharide
Method, include the following steps：1) fresh dendrobium candidum stem is mixed with water and carries out historrhexis, obtain dendrobium candidum slurries；
2) by the dendrobium candidum slurries obtained in step 1) and it is equivalent to raw material 10~20 times of fresh dendrobium candidum stem, 80~100 DEG C of volume
Water mixing carry out at least once polysaccharide elute, obtain eluent；3) eluent is filtered, filtrate and filter residue are obtained；It is described
It includes Dendrobium officinale polysaccharide to be in filtrate.
Preferably, the elution of polysaccharide described in step 2) includes the elution of the first polysaccharide and the elution of the second polysaccharide；First polysaccharide is washed
The first polysaccharide eluent is obtained after de-；It filters the first polysaccharide eluent and obtains the first filtrate and the first filter residue；By described
One filter residue is mixed with water carries out the second polysaccharide elution, obtains the second eluent；Second eluent is filtered, the second filtrate is obtained
With the second filter residue；First filtrate and the second filtrate are mixed, Dendrobium officinale polysaccharide is obtained.
Preferably, the polyoses content of fresh dendrobium candidum stem described in step 1) is more than 30wt%, the fresh iron sheet
The length of stem of noble dendrobium stalk is 5~25cm.
Preferably, the mass ratio of fresh dendrobium candidum stem and water described in step 1) is 1：(2~8).
Preferably, with stirring during the historrhexis described in step 1), the rotating speed of the stirring is 2200~
The time of 3200r/min, the stirring are 2~10min.
Preferably, the polysaccharide elution described in step 2) and the second polysaccharide described in step 4) are adjoint during eluting
Stirring, the rotating speed of the stirring stand alone as 60~80r/min, and the time of the stirring stands alone as 8~30min.
Preferably, it is filtered into plate compression described in step 3) and step 5).
Preferably, the mesh number of the strainer of the plate compression is 300~1000 mesh.
Preferably, the pressure of the plate compression is 2~4kg/cm2。
Preferably, further include after first filtrate and the mixing mixing of the second filtrate：By the iron sheet stone being mixed to get
Dry measure used in former times polysaccharide solution is concentrated, dry, obtains iron-stone dry measure used in former times polysaccharide powder.
Preferably, the concentration is reduced pressure；The temperature of the reduced pressure is 50~80 DEG C, the reduced pressure
Vacuum degree is -0.05~-0.09MPa.
Beneficial effects of the present invention：The method of rapid extraction Dendrobium officinale polysaccharide of the present invention is with fresh dendrobium candidum stem
For raw material, dry dendrobium candidum step is omitted, saves the time；Simultaneously only using water as solvent extraction Dendrobium officinale polysaccharide, peace
Entirely, pollution-free；Method operating procedure of the present invention is simple, is suitble to extensive quickly production；It is recorded according to embodiment, it is described
The yield of Dendrobium officinale polysaccharide can reach 10% or more, and polyoses content can reach 73.81% in finished product.
Description of the drawings
Fig. 1 is the flow diagram of the method and step of rapid extraction Dendrobium officinale polysaccharide in embodiment 1.
Specific implementation mode
The present invention provides a kind of method of rapid extraction Dendrobium officinale polysaccharide, include the following steps fresh iron sheet stone 1)
Dry measure used in former times stem is mixed with water carries out historrhexis, obtains dendrobium candidum slurries；2) the dendrobium candidum slurries and phase that will be obtained in step 1)
When the water mixing in raw material 10~20 times of fresh dendrobium candidum stem, 80~100 DEG C of volume carries out polysaccharide elution at least once, acquisition
Eluent；3) eluent is filtered, filtrate and filter residue are obtained；It includes Dendrobium officinale polysaccharide to be in the filtrate.
The fresh dendrobium candidum stem described in the present invention is preferably biennial dendrobium candidum；The fresh dendrobium candidum
Stem is preferably the stem after fresh dendrobium candidum removal leaf.The present invention does not limit the source of the fresh dendrobium candidum stem
It is fixed, the dendrobium candidum of different sources can be used.The length of the fresh dendrobium candidum stem described in the present invention is preferably 5~25cm,
The sugar content of more preferably 10~20cm, the fresh dendrobium candidum stem are preferably greater than 30%.The fresh dendrobium candidum
The inherent moisture of stem is 75% or more；Described 75% or more inherent moisture ensures the effect of the fresh dendrobium candidum stem mashing
The reason of fruit, this is also using fresh dendrobium candidum, if the inherent moisture of dendrobium candidum is too low, beating results are poor.
Preferably fresh dendrobium candidum stem is cleaned before the historrhexis in the present invention；The cleaning is preferred
It is cleaned for recirculated water, the cleaning method preferably uses high pressure cleaning showers in specific industrial production；The high-pressure spraying
The pressure of cleaning is preferably 0.1~0.15MP, and the rotating speed of the high pressure cleaning showers is preferably 100~600rpm, described
The time of high pressure cleaning showers is preferably 30~90s.
The present invention, which mixes fresh dendrobium candidum stem with water, carries out historrhexis, obtains dendrobium candidum slurries.In the present invention
In, the mass ratio of the fresh dendrobium candidum and water is preferably 1：(2~8), more preferably 1：(4~7).In the present invention
In, with stirring during the historrhexis, the rotating speed of the stirring is preferably 2200~3200r/min, more preferably
2500~3000rpm；The time of the stirring is preferably 2~10min, more preferably 4~8min.In the industrial production, described
Historrhexis preferably uses historrhexis's machine, when using historrhexis's machine, the mass ratio of the fresh dendrobium candidum and water
Preferably 1：5, rotating speed is preferably 2800r/min, and the time is preferably 6min.The purpose of the stirring is in the present invention
Effectively it is crushed fresh dendrobium candidum stem so that the polysaccharide in fresh dendrobium candidum stem can adequately be contacted with water, is conducive to
The extraction of polysaccharide in the fresh dendrobium candidum stem.Dendrobium candidum is broken in the dendrobium candidum slurries of the acquisition in the present invention
The granularity organized afterwards is preferably less than 200 μm, is more preferably less than 150 μm；Iron in heretofore described dendrobium candidum slurries
The particle size range organized after the skin stem of noble dendrobium is broken makes the polysaccharide component in dendrobium candidum be easier to elute.
The method of rapid extraction Dendrobium officinale polysaccharide of the present invention is eluted including polysaccharide at least once, and the polysaccharide is washed
De- number is preferably 2 times.
The present invention by the dendrobium candidum slurries of acquisition and is equivalent to the fresh iron sheet stone of raw material after obtaining dendrobium candidum slurries
The water mixing of 10~20 times of volumes of dry measure used in former times stem carries out the first polysaccharide elution, obtains the first eluent.In the present invention, more than described first
In sugared elution process, the addition volume of water is preferably 12~18 times of the fresh dendrobium candidum stem of raw material, more preferably 14~
16 times.In the present invention 60~80r/ is preferably with stirring, the rotating speed of the stirring in the first polysaccharide elution process
Min, more preferably 65~75rpm；The time of the stirring is preferably 8~30min.The polysaccharide elution in the present invention
It is preferably carried out in dynamic extraction pot, in the industrial production, preferably in 6M3It is carried out in dynamic extraction pot, in 6M3Dynamic carries
It takes when being carried out in tank, the mass ratio of the dendrobium candidum slurries and water is preferably 1:15, speed of agitator is preferably 70rpm,
Mixing time is preferably 10min.
The present invention filters first eluent after obtaining the first eluent, obtains the first filtrate and the first filter residue.
It is heretofore described to be filtered into plate compression.The mesh number of the strainer of the plate compression is preferably 300~1000 mesh, more preferably
It is 400~800 mesh；The pressure of the plate compression is preferably 2~4kg/cm2, more preferably 3kg/cm2.In the present invention
In, contain Dendrobium officinale polysaccharide in first filtrate, there are the undissolved dendrobium candidums out in part in first filter residue
Polysaccharide.Accordingly, it is preferred that carrying out secondary polysaccharide elution to first filter residue.
The present invention, which mixes after obtaining the first filter residue, by first filter residue with water, carries out the second polysaccharide elution, obtains the
Two eluents.The mass volume ratio of the first filter residue and water is preferably 1 in the second polysaccharide elution in the present invention：(4~
10), more preferably 1：(5~8).Turn in the present invention with stirring, the stirring in the second polysaccharide elution process
Speed is preferably 60~80r/min, more preferably 65~75rpm；The time of the stirring is preferably 8~30min.At this
The elution of second polysaccharide described in invention preferably carries out in dynamic extraction pot, in the industrial production, preferably in 6M3Dynamic carries
It takes in tank and carries out, in 6M3When being carried out in dynamic extraction pot, the mass volume ratio of described first filter residue and water is preferably 1:6,
Speed of agitator is preferably 70rpm, and mixing time is preferably 10min.
In the present invention, after obtaining second eluent, second eluent is filtered, obtains the second filtrate and the
Two filter residues；The method of the method and Parameter Conditions and the first eluent of above-mentioned filtering of the second eluent of the filtering in the present invention
It is consistent with conditional parameter, it repeats no more again.
The temperature of water is independently 80~100 described in the polysaccharide elution in the present invention, the second polysaccharide elution step
DEG C, preferably 85~95 DEG C.It is starched respectively with fresh dendrobium candidum stem, dendrobium candidum using the water of above-mentioned temperature in the present invention
Liquid and the mixing of the first filter residue, can improve extraction efficiency, save extraction time.
First filtrate and the second filtrate are mixed after obtaining the second filtrate and obtain Dendrobium officinale polysaccharide by the present invention.
The Dendrobium officinale polysaccharide of the acquisition is the polysaccharide of solution state in the present invention；It is currently preferred to obtain above-mentioned solution shape
After the Dendrobium officinale polysaccharide of state, preferably iron-stone dry measure used in former times polysaccharide powder will be obtained after Dendrobium officinale polysaccharide concentration, drying.At this
Concentration described in invention is preferably concentrated under reduced pressure；The temperature of the reduced pressure is preferably 50~80 DEG C, more preferably
60~70 DEG C；The vacuum degree of the reduced pressure be preferably -0.05~-0.09MPa, more preferably -0.06~-
0.08MPa.In the present invention it is described reduced pressure preferably using economic benefits and social benefits outer circulation concentration tank carry out, when use economic benefits and social benefits outer circulation
When concentration tank is concentrated under reduced pressure, an effect temperature is preferably 60~80 DEG C, more preferably 75 DEG C；Two effect temperature are preferably
50~70 DEG C, more preferably 60 DEG C；Vacuum degree is preferably -0.05~-0.09MPa, more preferably -0.08MPa.At this
Reduced pressure described in invention stops when being preferably concentrated into proportion 1.05~1.10.
In the present invention, after the Dendrobium officinale polysaccharide concentration, it is more that acquisition iron-stone dry measure used in former times is dried in obtained concentrate
Icing Sugar end.The present invention is not particularly limited the drying means, using the drying means of this field routine；In the present invention
It is dry described in middle specific implementation process to be preferably spray-dried the spray drying preferably using drying machine with centrifugal spray
Group carries out；The inlet air temperature of the spray drying is preferably 170~210 DEG C, more preferably 180~200 DEG C, most preferably
It is 190 DEG C；The leaving air temp of the spray drying is preferably 90~130 DEG C, more preferably 100~120 DEG C, most preferably
It is 118 DEG C.In the present invention, the loading frequency of concentrate is sized preferably according to air inlet leaving air temp in the spray drying
It is 12~18Hz, more preferably 15Hz.The content of Dendrobium officinale polysaccharide is in the iron-stone dry measure used in former times polysaccharide powder that the present invention obtains
72~74%.
It is detailed to a kind of method progress of rapid extraction Dendrobium officinale polysaccharide provided by the invention with reference to embodiment
Illustrate, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
A kind of method of quick obtaining Dendrobium officinale polysaccharide, includes the following steps：
(1) it cleans：It is clear with flow circuit water using circulating water washer by biennial fresh dendrobium candidum stem 10kg
Wash clean.
(2) historrhexis：It is broken to carry out tissue for 5 times of amount aqueous solutions of addition in fresh dendrobium candidum stem obtained by step (1)
Broken, the rotating speed of setting historrhexis machine is 2800r/min, and time 6min obtains dendrobium candidum slurries.
(3) it elutes：The heat for being equivalent to 15 times of amounts of the fresh dendrobium candidum stem of raw material is added in slurries obtained by step (2)
Water.It is eluted in dynamic extraction pot, speed of agitator 70r/min, time 10min obtains the first eluent.
(4) it filters：The first eluent filtering that step (3) elution action is obtained, obtains the first filtrate and the first filter residue.
The preferred plate compression of mode of filtering, using WBK-400 stainless steel plate frame-type filters, 400 mesh filter clothes, pressure 0.2MPa.
The hot water of 6 times of amounts is added in the first filter residue, eluted, filtered according to step (3), (4), obtain the second filter
Liquid.
(5) it concentrates：The first filtrate that step (4) obtains and the second filtrate are merged into concentration, obtain concentrate.Concentration
Mode be to be concentrated under reduced pressure, preferably TN300L small-sized extraction concentration units are concentrated, and 65 DEG C of temperature, vacuum-are controlled
0.07MPa is concentrated into proportion 1.05 (temperature 60 C).
(6) dry：The concentrate that step (5) obtains is dried to solid powder to get Dendrobium officinale polysaccharide.Using LPG
Type drying machine with centrifugal spray is spray-dried, technological parameter setting：176 DEG C of inlet air temperature, 94 DEG C of leaving air temp, centrifugal rotational speed
For 400r/min.Finally obtain linen solid powder 1.12kg, yield 11.2%；The yield is more with dendrobium candidum
What Icing Sugar end obtained compared with the fresh dendrobium candidum stem of raw material.
The time that the present embodiment the method obtains Dendrobium officinale polysaccharide powder is 6h~10h, compared to using dry iron
It is more than hour to shorten 60 for the skin stem of noble dendrobium, and residue polyoses content reduces by 5% or more.The present embodiment the method is with the prior art with dry
Product are that the time-consuming and residue polyoses content situation comparison of the method for raw material is shown in Table 1.
1 the present embodiment the method for table takes with the prior art and residue polyoses content compares
The measurement result of sampled detection, this batch of Dendrobium officinale polysaccharide powder is：The content of Dendrobium officinale polysaccharide is
72.55%, ash content 5.60%, moisture 3.87%；The polyoses content of the dregs of a decoction is 6.34%, and detection foundation is version in 2015《China
Pharmacopeia》Detection method under first dendrobium candidum item.
Embodiment 2
A kind of method of quick obtaining Dendrobium officinale polysaccharide, includes the following steps：
(1) it cleans：It is clear with flow circuit water using circulating water washer by biennial fresh dendrobium candidum stem 200kg
Wash clean, production capacity 200kg/h.
(2) historrhexis：The aqueous solution that 5 times of amounts are added in fresh dendrobium candidum stem obtained by step (1) carries out tissue
Broken, the rotating speed of setting historrhexis machine is 2800r/min, time 6min, obtains dendrobium candidum slurries, weight about 1t, production
Can be 0.5t/h.
(3) it elutes：The heat for being equivalent to 15 times of amounts of the fresh dendrobium candidum stem of raw material is added in slurries obtained by step (2)
Water.In 6M3It is eluted in dynamic extraction pot, speed of agitator 70r/min, time 10min obtains the first eluent.
(4) it filters：The first eluent filtering that step (3) elution action is obtained, obtains the first filtrate and the first filter residue.
The mode of filtering is plate compression, and using plate and frame filter press, net formula filter cloth is 800 mesh, production capacity 1.5t/h.
The hot water for being equivalent to 6 times of the fresh dendrobium candidum stem of raw material amount are added in the first filter residue, according to step (3), (4) into
Row elution, filtering, obtain the second filtrate.
(5) it concentrates：The first filtrate that step (4) obtains and the second filtrate are merged into concentration, obtain concentrate.Using
Economic benefits and social benefits outer circulation concentration tank is concentrated, one 75 DEG C of temperature of effect of control, vacuum degree -0.06MPa, two effect temperature 60 Cs, vacuum degree -
0.08MPa is concentrated into proportion 1.10 (temperature 60 C), and production capacity is calculated as 1.8t/h with evaporation capacity.
(6) dry：The concentrate that step (5) obtains is dried to solid powder to get Dendrobium officinale polysaccharide.Using
ZPL150-00 type drying machine with centrifugal spray groups are spray-dried, and 190 DEG C of inlet air temperature, 118 DEG C of leaving air temp, charging is arranged
Frequency is 15Hz, spray drying time about 3h.Finally obtain linen solid powder 24.4kg, yield 12.2%；The yield
It is to be obtained compared with the fresh dendrobium candidum stem of raw material with Dendrobium officinale polysaccharide powder.
After testing, the measurement result of this batch of Dendrobium officinale polysaccharide powder is：The content of Dendrobium officinale polysaccharide be 73.81%,
Ash content 5.19%, moisture 4.26%；The polyoses content of the dregs of a decoction is 6.83%, and detection foundation is version in 2015《Chinese Pharmacopoeia》First
Detection method under portion's dendrobium candidum item.According to example, large-scale production is operated with 200kg/ batches, whole process total time-consuming
Only need 14~20 hours.
Embodiment 3
(1) it cleans：Biennial fresh dendrobium candidum stem 400g is followed using test-type circulating water washer with flowing
Ring water cleans up.
(2) historrhexis：It is broken to carry out tissue for 5 times of amount aqueous solutions of addition in fresh dendrobium candidum stem obtained by step (1)
Broken, the rotating speed of setting Mini Tissue crusher is 2600r/min, and time 7min obtains dendrobium candidum slurries.
(3) it elutes：The heat for being equivalent to 15 times of amounts of the fresh dendrobium candidum stem of raw material is added in slurries obtained by step (2)
Water.It is eluted in tank, the rotating speed that agitating paddle is arranged is 75r/min, and time 15min obtains the first eluent.
(4) it filters：Using 400 mesh filter clothes, the first eluent that step (3) elution action is obtained filters, and obtains first
Filtrate and the first filter residue.
The hot water of 6 times of amounts is added in the first filter residue, eluted, filtered according to step (3), (4), obtain the second filter
Liquid.
(5) it concentrates：The first filtrate that step (4) obtains and the second filtrate are merged into concentration, obtain concentrate.Concentration
Mode be to be concentrated under reduced pressure, preferably rotary evaporator is concentrated, and controls 65 DEG C of temperature, vacuum -0.09MPa is concentrated into 1kg
(temperature 60 C).
(6) dry：The concentrate that step (5) obtains is dried to solid powder to get Dendrobium officinale polysaccharide.Test-type from
Heart spray dryer is spray-dried, technological parameter setting：170 DEG C of inlet air temperature, 90 DEG C of leaving air temp, centrifugal rotational speed are
400r/min.After dry 10min, linen solid powder 49.2g, yield 12.3% are finally obtained；The yield is
It is obtained compared with the fresh dendrobium candidum stem of raw material with Dendrobium officinale polysaccharide powder.
The measurement result of sampled detection, this batch of Dendrobium officinale polysaccharide powder is：The content of Dendrobium officinale polysaccharide is
73.02%, ash content 5.42%, moisture 4.26%；The polyoses content of the dregs of a decoction is 6.21%, and detection foundation is version in 2015《China
Pharmacopeia》Detection method under first dendrobium candidum item.
By above-described embodiment it is found that the method for rapid extraction Dendrobium officinale polysaccharide provided by the invention is compared with existing method
Water is only added as solvent, acid-base solution, organic solvent, biochemical reagents etc. are not added, it is cost-effective, pollution is reduced, with this
The Dendrobium officinale polysaccharide that invention the method obtains, quality safety are reliable.
The present invention shortens entirety as raw material using fresh dendrobium candidum stem compared with other methods using dry product
Operating time, reduce loss.For example, large-scale production is calculated with 200kg/ batches, need dendrobium candidum being cut into the left sides length 5cm
Right short section, then iron-sheet dendrobe fresh product is dried to by dry product, 100 DEG C of drying temperature using baking oven, the time needs 8~12 hours, disappears
Consume 2~2.5 tons of steam.
Method of the present invention is and existing by the way of cleaning, historrhexis, elution, filtering, concentration and drying
Method is simple compared to operating procedure.Mashing, extracted many times, concentration, alcohol precipitation, centrifugation, macroporous resin adsorption is omitted after purification again
The troublesome operations such as concentration, dry, save the time.It using the method for the present invention, is calculated with 200kg/ batches, total time-consuming only needs 14~20
The time of hour, meet the requirement quickly produced.
The method of extraction Dendrobium officinale polysaccharide of the present invention, using historrhexis's machine, by iron in dendrobium candidum slurries
The granularity organized after the skin stem of noble dendrobium is broken is less than 150 μm, and the polysaccharide component in dendrobium candidum is made to be easier to elute.
Method of the present invention can effectively improve the recovery rate of Dendrobium officinale polysaccharide, until 10% or more.By Chinese Pharmacopoeia
Methods in Determination of Polysaccaride Content is detected as defined in 2015 editions, and the content of Dendrobium officinale polysaccharide can reach 70% or more in product,
The bioaccumulation efficiency of active ingredient is high.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (11)
1. a kind of method of rapid extraction Dendrobium officinale polysaccharide, includes the following steps：
1) fresh dendrobium candidum stem is mixed with water and carries out historrhexis, obtain dendrobium candidum slurries；
2) by the dendrobium candidum slurries obtained in step 1) and be equivalent to 10~20 times of volumes 80 of the fresh dendrobium candidum stem of raw material~
100 DEG C of water mixing carries out polysaccharide at least once and elutes, and obtains polysaccharide eluent；
3) the polysaccharide eluent is filtered, filtrate and filter residue are obtained；It includes Dendrobium officinale polysaccharide to be in the filtrate.
2. according to the method described in claim 1, it is characterized in that, polysaccharide washing steps described in step 2) is twice, specifically
Including：First polysaccharide elutes and the elution of the second polysaccharide；The first polysaccharide eluent is obtained after the elution of first polysaccharide；Filter described first
Polysaccharide eluent obtains the first filtrate and the first filter residue；
First filter residue is mixed with 80~100 DEG C of water and carries out the second polysaccharide elution, obtains the second eluent；Described in filtering
Second eluent obtains the second filtrate and the second filter residue；
First filtrate and the second filtrate are mixed, Dendrobium officinale polysaccharide is obtained.
3. according to the method described in claim 1, it is characterized in that, the polyoses content of fresh dendrobium candidum stem described in step 1)
More than 30wt%, the length of the fresh dendrobium candidum stalk is 5~25cm.
4. according to the method described in claim 1, it is characterized in that, the quality of fresh dendrobium candidum stem and water described in step 1)
Than being 1：(2~8).
5. method according to claim 1 or 4, which is characterized in that adjoint during historrhexis described in step 1)
The rotating speed of stirring, the stirring is 2200~3200r/min, and the time of the stirring is 2~10min.
6. according to the method described in claim 2, it is characterized in that, the first polysaccharide elution described in step 2) and described the
The rotating speed of the adjoint stirring during eluting of two polysaccharide, the stirring stands alone as 60~80r/min, and the time of the stirring is independent
For 8~30min.
7. according to the method described in claim 2, it is characterized in that, described be filtered into plate compression.
8. the method according to the description of claim 7 is characterized in that the mesh number of the strainer of the plate compression is 300~1000
Mesh.
9. method according to claim 7 or 8, which is characterized in that the pressure of the plate compression is 2~4kg/cm2。
10. according to the method described in claim 2, it is characterized in that, further including after first filtrate and the mixing of the second filtrate：
It is the Dendrobium officinale polysaccharide solution being mixed to get is concentrated, dry, obtain iron-stone dry measure used in former times polysaccharide powder.
11. according to the method described in claim 10, it is characterized in that, the concentration is reduced pressure；The reduced pressure
Temperature is 50~80 DEG C, and the vacuum degree of the reduced pressure is -0.05~-0.09MPa.
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| CN113512128A (en) * | 2021-05-13 | 2021-10-19 | 海南云皓生物科技有限公司 | Dendrobium polysaccharide and preparation method thereof |
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