CN108310962B - Preparation method of composite microbial deodorant, deodorant and using method of deodorant - Google Patents

Preparation method of composite microbial deodorant, deodorant and using method of deodorant Download PDF

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CN108310962B
CN108310962B CN201810327820.XA CN201810327820A CN108310962B CN 108310962 B CN108310962 B CN 108310962B CN 201810327820 A CN201810327820 A CN 201810327820A CN 108310962 B CN108310962 B CN 108310962B
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田丹
于景成
靳国良
丁雪梅
田艺伟
辛凯
韩光光
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Beijing Haoye Tongyu Technology Co ltd
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Abstract

The preparation method of the composite microbial deodorant comprises the following steps: preparing an environment-friendly enzyme, preparing a photosynthetic bacteria seed liquid, preparing a saccharomycete seed liquid, preparing a lactic acid bacteria seed liquid, preparing an actinomycete seed liquid, preparing a bacillus seed liquid and preparing an acetic acid bacteria seed liquid, mixing the photosynthetic bacteria seed liquid, the saccharomycete seed liquid, the lactic acid bacteria seed liquid, the actinomycete seed liquid, the bacillus seed liquid and the acetic acid bacteria seed liquid according to a certain proportion, placing the mixture in a brown sugar culture medium for culturing to obtain a mixed microorganism fermentation liquid, and finally uniformly mixing the environment-friendly enzyme and the mixed microorganism fermentation liquid according to a volume ratio of 0.5-1: 1-1.5 to obtain the composite microorganism deodorant; the microorganisms in the composite microbial deodorant mutually promote and cooperate to effectively decompose biological organic matters generating odor, and the composite microbial deodorant has a good deodorization effect and a wide application range.

Description

Preparation method of composite microbial deodorant, deodorant and using method of deodorant
Technical Field
The invention relates to the field of deodorants, in particular to a preparation method of a compound microorganism deodorant compounded by environment-friendly ferment and multiple microorganisms, a deodorant and a using method thereof.
Background
Malodorous gases can be classified into 5 types, depending on the chemical composition of the gas: one is a sulfur-containing compound, e.g. H2S、SO2Thiols, thioethers; nitrogen-containing compounds such as amines, amides, indoles; thirdly halogens and derivatives such as chlorine, halogenated hydrocarbons; fourthly, hydrocarbons, such as alkane, alkene, alkyne and aromatic hydrocarbon; fifthly, oxygen-containing organic matters such as alcohol, phenol, aldehyde, ketone, organic acid and the like. The gas chromatography detection shows that the main component of most malodorous gas is ammonia (NH)3) And hydrogen sulfide (H)2S)。
At present, there are three main types of methods for treating malodorous gases, namely physical methods, chemical methods and biological methods. The physical deodorization method has strong selectivity to odor components, has slow deodorization speed, and is suitable for small spaces such as refrigerators, freezers and the like. Although the chemical deodorization method can thoroughly eliminate the offensive odor, the deodorization cost is high and secondary pollution is easy to generate. The biological method for treating the malodorous gas as a novel malodorous gas pollution control technology has the advantages of good effect, wide application range, simple required equipment, easy management, convenient maintenance, no secondary pollution and the like, is generally concerned in the research and application of malodorous prevention at home and abroad, and shows good development space and application prospect.
The invention patent with application number 201710138304.8 discloses a compound microorganism deodorant, which is composed of a growth promoter and a microbial inoculum and can effectively remove various common peculiar smells and smells in production and life. However, the growth promoter and the microbial inoculum in the deodorant are produced by extracting plants, stir-frying brown sugar and cultivating various microorganisms, the production process is complex, and the production cost of the biological deodorant is increased. The invention patent with application number 01129774.3 discloses a microbial deodorant, a preparation method and application thereof, wherein the microbial deodorant is prepared by mixing and fermenting two yeasts of a yeast genus of saccharomyces, namely a yeast capable of producing a protein and a yeast capable of producing a small ellipse, so that the application range of the deodorant is narrow.
Disclosure of Invention
The invention aims to provide a preparation method of a composite microbial deodorant, the deodorant and a use method thereof, which have the advantages of good deodorization effect, wide application range and low production cost, aiming at the defects and the shortages in the prior art.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: it includes: the method comprises the following steps:
preparation of environment-friendly enzyme
Preparing 9kg of brown sugar or molasses, 1kg of yeast extract, 20-30kg of fruit or fruit peel and 100L of purified water; dissolving brown sugar or molasses and yeast extract in boiled water at the temperature of not higher than 40 ℃ to form sugar water, cleaning fruits or fruit peels with tap water, cleaning a ferment fermentation tank, and rubbing the ferment fermentation tank with 60-80% ethanol; then sequentially adding fruits or fruit peels, the sugar water and purified water, and sealing the fermentation tank for natural fermentation for 30-100 days; the total number of viable bacteria in the obtained environment-friendly enzyme solution is 3.0 multiplied by 1010cfu/ml—5.0×1010cfu/ml, and the PH value is 3.5-4;
(2) preparing photosynthetic bacteria seed liquid:
(a) culture of photosynthetic bacteria in slant culture medium
Sequentially adding 3.5g of sodium acetate, 1g of ammonium chloride, 0.1g of magnesium chloride, 0.1g of calcium chloride, 0.4g of monopotassium phosphate, 0.6g of dipotassium hydrogen phosphate, 0.1g of yeast extract and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, then adding the purified water to the constant volume of 1000mL, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then photosynthetic bacteria strains are purchased and placed into the bottle to be shaken uniformly, then the photosynthetic bacteria strains are inoculated on the slant culture medium by using an inoculating loop in a high density manner, and the activated photosynthetic bacteria strains are obtained after activation for 18-24 hours at the temperature of 30-33 ℃;
(b) preparation of photosynthetic bacteria in a liquid culture medium:
adding 3g of sodium acetate, 2g of ammonium chloride, 0.1g of sodium chloride, 0.05g of calcium chloride, 0.5g of monopotassium phosphate, 0.3g of dipotassium phosphate, 0.2g of magnesium sulfate, 0.02g of manganese sulfate, 0.002g of ferric trichloride and 0.1g of yeast extract into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated photosynthetic bacteria strain in the step (a) into the liquid culture medium in the step (b) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table at 100-150 r/min for 40-50 h at the temperature of 34-38 ℃, then the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml photosynthetic bacteria seed solution;
(3) preparation of yeast seed liquid
(c) Preparation of slant culture medium for yeast
Adding 20g of glucose, 10g of yeast extract, 20g of peptone and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then yeast strains are purchased and placed into the bottle to be shaken up, then the yeast strains are inoculated on the slant culture medium by an inoculating loop in high density and activated for 18-24 h at the temperature of 30-33 ℃, and the activated yeast strains are obtained;
(d) preparation of yeast in liquid culture medium
Adding 9g of glucose, 45g of molasses, 34-38 g of bran, corn DDGS3g, 2.75g of cottonseed meal, 0.75g of yeast extract and 0g of ammonium sulfate into a 3L beaker in sequence8g, 3g of monopotassium phosphate, 0.3g of magnesium sulfate and 0.3g of calcium chloride, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated saccharomycete strain in the step (c) to the liquid culture medium in the step (d) for culture, wherein the cultured liquid is called bacterial suspension, culturing the bacterial suspension in a shaker at the temperature of 34-38 ℃ for 40-50 h at the speed of 100-150 r/min, detecting the optical density of each bacterial suspension OD600, stopping the culture when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, and obtaining the viable bacteria with the viable bacteria number of 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of yeast seed solution;
(4) preparation of lactic acid bacteria seed liquid
(e) Preparation of lactic acid bacteria in a slant culture medium:
sequentially adding 5g of peptone, 2g of corn steep liquor, 40g of sucrose, 5g of sodium acetate, 10g of sodium chloride, 5g of magnesium sulfate and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing lactobacillus strains, putting the lactobacillus strains into the bottle, shaking the bottle uniformly, respectively inoculating the lactobacillus strains on the slant culture medium with high density by using inoculating loops, and activating for 18 to 24 hours at the temperature of 30 to 33 ℃ to obtain activated lactobacillus strains;
(f) preparation of lactic acid bacteria in liquid culture medium
Sequentially adding 10g of peptone, 8g of beef extract, 4g of yeast extract, 2g of diammonium hydrogen citrate, 20g of glucose, 801g of tween, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate and 0.04g of manganese sulfate into a 3L beaker, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.0 +/-0.2; culturing the prepared liquidPackaging the base into 250ml conical bottles, sealing and putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; inoculating the activated lactobacillus strain in the step (e) into the liquid culture medium in the step (f) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 100-150 r/min for 40-50 h at the temperature of 34-38 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of lactobacillus seed solution;
(5) preparation of Actinomycetes seed liquid
(g) Preparing an actinomycete slant culture medium:
adding 3g of beef extract, 10g of peptone, 5g of sodium chloride and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 15g of agar, dissolving with 1000mL of purified water, and adjusting the pH value to 7.2 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of sterile water by using a sterile micropipette, putting the sterile water into a bottle, purchasing actinomycete strains, putting the actinomycete strains into the bottle, shaking the actinomycete strains uniformly, inoculating the actinomycete strains on the slant culture medium by using an inoculating loop in a high-density manner, and activating the actinomycete strains for 18 to 24 hours at the temperature of between 30 and 33 ℃ to obtain activated actinomycete strains;
(h) preparation of actinomycete liquid culture medium:
adding 10g of glucose, 4g of yeast extract, 4g of peptone, 4g of dipotassium hydrogen phosphate, 2g of potassium dihydrogen phosphate and 0.5g of magnesium sulfate into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated actinomycete strain in the step (g) to the liquid culture medium in the step (h) for culture, wherein the cultured liquid is called bacterial suspension, and the bacterial suspension is shaken at the temperature of 34-38 ℃ at 100-150 r/minCulturing in bed for 40-50 h, detecting optical density of OD600 of each bacterial suspension, stopping culturing when the optical density of OD600 of each bacterial suspension is equal to or greater than 4.0, and obtaining viable bacteria with number of 3.0 × 108cfu/ml～4.0×108cfu/ml actinomycete seed solution;
(5) preparation of Bacillus seed liquid
(i) Preparation of a bacillus slant culture medium:
sequentially adding 10g of peptone, 15g of beef extract, 15g of sodium chloride and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 18g of agar, diluting to 1000mL with purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing bacillus strains, putting the bacillus strains into the bottle, shaking the bacillus strains uniformly, inoculating the bacillus strains on the slant culture medium at high density by using an inoculating loop, and activating for 18-24 h at the temperature of 30-33 ℃ to obtain activated bacillus strains;
(j) preparation of a bacillus liquid culture medium:
adding 20g of corn starch, 10g of corn steep liquor, 5g of ammonium sulfate, 2g of monopotassium phosphate, 1g of magnesium sulfate and 1g of sodium chloride into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated bacillus strain in the step (i) into the liquid culture medium in the step (j) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 100-150 r/min for 40-50 h at the temperature of 34-38 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of bacillus seed solution;
(6) preparation of acetic acid bacteria seed liquid
(k) Preparation of acetic acid bacteria slant culture medium: adding 10g of glucose, 10g of yeast powder, 10g of calcium carbonate and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 25g of agar, adding purified water to reach the constant volume of 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then acetic acid bacteria strains are purchased and placed into the bottle to be shaken uniformly, then inoculating the acetic acid bacteria strains on the slant culture medium with an inoculating loop in a high density, and activating for 18-24 h at the temperature of 30-33 ℃ to obtain activated acetic acid bacteria strains;
(l) Preparation of acetic acid bacteria liquid culture medium:
adding 10g of glucose, 10g of yeast powder and 10mL of edible alcohol into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 5.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated acetic acid bacteria strain in the step (k) into the liquid culture medium in the step (l) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaker at the temperature of 34-38 ℃ at the speed of 100-150 r/min for 40-50 h, then the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml acetic acid bacteria seed liquid;
(7) preparation of Mixed microbial fermentation broth
Dissolving 1kg of brown sugar in 9L of distilled water to obtain a brown sugar culture medium; respectively taking 20-40% of photosynthetic bacteria seed liquid, 10-30% of saccharomycete seed liquid, 5-15% of lactic acid bacteria seed liquid, 5-15% of actinomycete seed liquid and 5-15% of bacillus in the steps (2) to (6)The bacterium seed liquid and 5 to 15 percent of acetic acid bacterium seed liquid are mixed into a compound microorganism to be inoculated into the brown sugar culture medium, and the mixture is sealed and fermented for 7 to 10 days at the temperature of between 20 and 25 ℃ to obtain the viable bacteria with the number of 3.0 multiplied by 1010cfu/ml～4.0×1010cfu/ml of mixed microbial fermentation broth;
(8) mixing of environmental-friendly enzyme and microbial fermentation broth
Filtering the environment-friendly enzyme obtained in the step (1), and uniformly mixing the environment-friendly enzyme and the mixed microorganism fermentation liquor obtained in the step (7) according to the volume ratio of 0.5-1: 1-1.5 to obtain a composite microorganism deodorant; the total number of viable bacteria in the obtained composite microbial deodorant is 3.5 × 1010cfu/ml/ml～4.5×1010cfu/ml；
The invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the fruit or the peel is one or more of apple, pear, peach, pineapple, sugarcane, orange, watermelon, melon or the peel, and the purified water is distilled water;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the photosynthetic strain is Rhodopseudomonas palustris (Rhodopseudomonas palustris) which is researched and preserved by institute of microbiology of Chinese academy of sciences, the strain source is Chinese, and the preservation number is as follows: CGMCC No.1.8929, with preservation date of 2014, 12 months and 16 days;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the yeast strain is Rhodotorula mucilaginosa (Rhodotorula mucor) preserved by China general microbiological culture preservation management center, the strain source is China, and the preservation number is as follows: CGMCC No.2.4008, the preservation date is 2010, 7 months and 13 days;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the Lactobacillus strain is acidophilic Lactobacillus (Lactobacillus acidophilus) preserved by the China agricultural microbial strain preservation management center, the strain source is China, and the preservation number is as follows: ACCC10637, with a preservation date of 2013, 10 months and 14 days;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the actinomycete is rumen-inhabiting actinomycete (Actinomyces ruminicola) preserved by China general microbiological culture preservation management center, the actinomycete comes from China, and the preservation number is as follows: CGMCC1.5030, with preservation date of 6 months and 3 days in 2005;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the Bacillus species is Bacillus thuringiensis (Bacillus thuringiensis) preserved by China general microbiological culture Collection center, the strain source is China, and the preservation number is as follows: CGMCC 1.4671, with preservation date of 11 months and 1 day in 2013;
the invention discloses a preparation method of a composite microbial deodorant, which comprises the following steps: the acetic acid strain is Acetobacter pasteurianus (Acetobacter pasteurianus) preserved by China center for industrial microorganism strain preservation management, the strain source is China, and the preservation number is as follows: SICC 1.3, and the preservation date is 11 months and 15 days in 2008;
the compound microbial deodorant prepared by the preparation method of the compound microbial deodorant of the invention;
the invention discloses a using method of a composite microbial deodorant, which comprises the following steps: the composite microbial deodorant is diluted by 10-20 times with water, and is sprayed in the air with odor by spraying equipment, wherein the spraying height is not less than 2m, and the using amount is 0.05-0.5L/m2。
The invention has the beneficial effects that:
1. the fruit raw material of the environment-friendly enzyme is recycled waste fruits or pericarp garbage, so that the cost of the raw material is reduced, and a certain social benefit is generated;
2. the preparation of the microbial fermentation liquid adopts a mixed fermentation process, so that the fermentation efficiency is improved, the production period is shortened, and the production cost is further reduced;
3. according to the composite microbial deodorant disclosed by the invention, due to the addition of the environment-friendly enzyme, beneficial microbial populations in the deodorant are more diverse, the total quantity of live bacteria is greatly increased, the microorganisms mutually promote and cooperatively grow, biological organic matters generating odor can be effectively decomposed, and H is absorbed and utilized2S、SO2Malodorous gases such as mercaptan, ammonia, amines and the like are used as nutrient substances, and the deodorant effect is good and the application range is wide.
The present invention will be described in detail with reference to the following embodiments in order to make the aforementioned objects, features and advantages of the invention more comprehensible. It will be appreciated by those skilled in the art that any equivalent modifications and alterations of the present invention are within the scope of the present invention and are not to be limited by the specific embodiments disclosed below.
Drawings
FIG. 1 is a list of the deposited bacterial species in the preparation method of the composite microbial deodorant of the present invention.
Detailed Description
Example 1
The preparation method of the composite microbial deodorant comprises the following steps: the method comprises the following steps:
(1) preparation of environment-friendly enzyme
Preparing 9kg of brown sugar, 1kg of yeast extract, 30kg of pineapple peel and 100L of purified water; dissolving brown sugar and yeast extract in boiling water at a temperature of not higher than 40 deg.C to obtain sugar water, cleaning pineapple peel with tap water, cleaning ferment fermentation tank, and wiping with 75% ethanol; then sequentially adding pineapple peel, the sugar water and purified water, sealing the fermentation tank, and naturally fermenting for 90 days; the total number of viable bacteria in the obtained environment-friendly enzyme solution is 4.2 multiplied by 1010cfu/ml, pH 3.9;
(2) preparing photosynthetic bacteria seed liquid:
(a) culture of photosynthetic bacteria in slant culture medium
Sequentially adding 3.5g of sodium acetate, 1g of ammonium chloride, 0.1g of magnesium chloride, 0.1g of calcium chloride, 0.4g of monopotassium phosphate, 0.6g of dipotassium hydrogen phosphate, 0.1g of yeast extract and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, then adding the purified water to the constant volume of 1000mL, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, using an aseptic micropipette to absorb 50 mu L of aseptic water and put into a bottle, purchasing photosynthetic bacteria strains and putting into the bottle to shake uniformly, then using an inoculating loop to inoculate the photosynthetic bacteria strains on the slant culture medium at high density, and activating for 20h under the condition of 32 ℃ to obtain activated photosynthetic bacteria strains;
(b) preparation of photosynthetic bacteria in a liquid culture medium:
adding 3g of sodium acetate, 2g of ammonium chloride, 0.1g of sodium chloride, 0.05g of calcium chloride, 0.5g of monopotassium phosphate, 0.3g of dipotassium phosphate, 0.2g of magnesium sulfate, 0.02g of manganese sulfate, 0.002g of ferric trichloride and 0.1g of yeast extract into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated photosynthetic bacteria strain in the step (a) into the liquid culture medium in the step (b) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 120r/min for 48 hours at the temperature of 35 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml photosynthetic bacteria seed solution;
(3) preparation of yeast seed liquid
(c) Preparation of slant culture medium for yeast
Adding 20g of glucose, 10g of yeast extract, 20g of peptone and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 μ L of sterile water with a sterile micropipette, placing into a bottle, purchasing yeast strains, placing into the bottle, shaking, inoculating the yeast strains on the slant culture medium with an inoculating loop at high density, and activating at 32 deg.C for 20 hr to obtain activated yeast strains;
(d) preparation of yeast in liquid culture medium
To 3LAdding 9g of glucose, 45g of molasses, 34-38 g of bran, corn DDGS3g, 2.75g of cottonseed meal, 0.75g of yeast extract, 0.8g of ammonium sulfate, 3g of monopotassium phosphate, 0.3g of magnesium sulfate and 0.3g of calcium chloride into a beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated yeast strain in the step (c) into the liquid culture medium in the step (d) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a 120r/min shaking table for 48 hours at the temperature of 35 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of yeast seed solution;
(4) preparation of lactic acid bacteria seed liquid
(e) Preparation of lactic acid bacteria in a slant culture medium:
sequentially adding 5g of peptone, 2g of corn steep liquor, 40g of sucrose, 5g of sodium acetate, 10g of sodium chloride, 5g of magnesium sulfate and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing lactobacillus strains, putting the lactobacillus strains into the bottle, shaking the bottle uniformly, respectively inoculating the lactobacillus strains on the slant culture medium by using inoculating loops at high density, and activating for 18-24 h at 32 ℃ to obtain activated lactobacillus strains;
(f) preparation of lactic acid bacteria in liquid culture medium
Sequentially adding 10g of peptone, 8g of beef extract, 4g of yeast extract, 2g of diammonium hydrogen citrate, 20g of glucose, 801g of tween, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate and 0.04g of manganese sulfate into a 3L beaker, and dissolving with purified waterAnd the volume is fixed to 1000mL, and the PH value is adjusted to 6.0 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated lactobacillus strain in the step (e) into the liquid culture medium in the step (f) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 120r/min for 48 hours at the temperature of 35 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of lactobacillus seed solution;
(5) preparation of Actinomycetes seed liquid
(g) Preparing an actinomycete slant culture medium:
adding 3g of beef extract, 10g of peptone, 5g of sodium chloride and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 15g of agar, dissolving with 1000mL of purified water, and adjusting the pH value to 7.2 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of sterile water by using a sterile micropipette, putting the sterile water into a bottle, purchasing actinomycete strains, putting the actinomycete strains into the bottle, shaking the actinomycete strains uniformly, inoculating the actinomycete strains on the slant culture medium by using an inoculating loop in a high-density manner, and activating the actinomycete strains for 20 hours at the temperature of 32 ℃ to obtain activated actinomycete strains;
(h) preparation of actinomycete liquid culture medium:
adding 10g of glucose, 4g of yeast extract, 4g of peptone, 4g of dipotassium hydrogen phosphate, 2g of potassium dihydrogen phosphate and 0.5g of magnesium sulfate into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated actinomycete strain in the step (g) to the liquid culture medium in the step (h) for culture, wherein the cultured liquid is called bacterial suspension, and the bacterial suspension is cultured in a shaking table of 120r/min at the temperature of 35 DEG CAfter 48h of cultivation, detecting the optical density of each bacterial suspension OD600, stopping cultivation when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, and obtaining viable bacteria with the number of 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml actinomycete seed solution;
(5) preparation of Bacillus seed liquid
(i) Preparation of a bacillus slant culture medium:
sequentially adding 10g of peptone, 15g of beef extract, 15g of sodium chloride and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 18g of agar, diluting to 1000mL with purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing bacillus strains, putting the bacillus strains into the bottle, shaking the bacillus strains uniformly, inoculating the bacillus strains on the slant culture medium by using an inoculating loop in a high-density manner, and activating for 18-24 hours at the temperature of 32 ℃ to obtain activated bacillus strains;
(j) preparation of a bacillus liquid culture medium:
adding 20g of corn starch, 10g of corn steep liquor, 5g of ammonium sulfate, 2g of monopotassium phosphate, 1g of magnesium sulfate and 1g of sodium chloride into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated bacillus strain in the step (i) into the liquid culture medium in the step (j) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 120r/min for 48 hours at the temperature of 35 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 x 108cfu/ml～4.0×108cfu/ml of bacillus seed solution;
(6) preparation of acetic acid bacteria seed liquid
(k) Preparation of acetic acid bacteria slant culture medium:
adding 10g of glucose, 10g of yeast powder, 10g of calcium carbonate and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 25g of agar, adding purified water to reach the constant volume of 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, placing into a high pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 μ L of sterile water with a sterile micropipette, placing into a bottle, purchasing acetic acid bacteria, placing into the bottle, shaking, inoculating acetic acid bacteria with inoculating loop at high density on the slant culture medium, and activating at 32 deg.C for 20 hr to obtain activated acetic acid bacteria;
(l) Preparation of acetic acid bacteria liquid culture medium:
adding 10g of glucose, 10g of yeast powder and 10mL of edible alcohol into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 5.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, placing into a high-pressure steam sterilization pot, and pressurizing at 121 deg.C for 15 min; inoculating the activated acetic acid bacteria strain in the step (k) into the liquid culture medium in the step (l) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a 120r/min shaking table for 48 hours at the temperature of 35 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml acetic acid bacteria seed liquid;
(7) preparation of Mixed microbial fermentation broth
Dissolving 1kg of brown sugar in 9L of distilled water to obtain a brown sugar culture medium; respectively taking 40% of photosynthetic bacteria seed liquid, 20% of saccharomycete seed liquid, 15% of lactic acid bacteria seed liquid, 10% of actinomycete seed liquid, 10% of bacillus seed liquid and 5% of acetic acid bacteria seed liquid in the steps (2) to (6) to mix into a compound microorganism, inoculating the compound microorganism into the brown sugar culture medium, and sealing the compound microorganism at the temperature of 20 DEG CFermenting for 8 days to obtain viable bacteria with a count of 3.4 × 1010cfu/ml of mixed microbial fermentation broth;
(8) mixing of environmental-friendly enzyme and microbial fermentation broth
Filtering the environment-friendly ferment obtained in the step (1), and uniformly mixing the environment-friendly ferment with the mixed microorganism fermentation liquor obtained in the step (7) according to the volume ratio of 1:1 to obtain a composite microorganism deodorant; the total number of viable bacteria in the obtained composite microbial deodorant is 3.5 × 1010cfu/ml/ml。
Example 2
Preparation of composite microbial deodorant
(1) Preparation of environment-friendly enzyme
18kg of molasses, 2kg of yeast extract, 20kg of melon, 20kg of watermelon and 200L of purified water; cleaning the recovered melon and watermelon with tap water, cleaning the ferment fermentation tank, wiping with 75% ethanol, dissolving molasses and yeast extract with boiling water at a temperature not higher than 40 deg.C to obtain sugar water, sequentially adding melon and watermelon, sugar water and purified water, and sealing the fermentation tank for natural fermentation for 1 month; the total number of viable bacteria in the obtained environment-friendly ferment is 3.8 multiplied by 1010cfu/ml, pH 3.6;
(2) - (6) preparation of photosynthetic bacteria group, yeast, lactic acid bacteria, actinomycetes, bacillus and acetic acid bacteria species as in example 1.
(7) Preparation of Mixed microbial fermentation broth
Dissolving 1kg of brown sugar in 9L of distilled water to obtain a brown sugar culture medium; respectively taking 30% of photosynthetic bacteria seed liquid, 30% of microzyme seed liquid, 20% of lactic acid bacteria seed liquid, 10% of actinomycetes seed liquid, 5% of bacillus seed liquid and 5% of acetic acid bacteria seed liquid in the steps (2) to (6) to mix into a composite microorganism, inoculating the composite microorganism into the brown sugar culture medium, and hermetically fermenting for 8 days at the temperature of 20 ℃ to obtain the composite microorganism with the viable bacteria number of 3.5 multiplied by 1010cfu/ml of mixed microbial fermentation broth;
(8) mixing of environmental-friendly enzyme and microbial fermentation broth
Filtering the environment-friendly ferment obtained in the step (1), and mixing the environment-friendly ferment with the mixed microorganism fermentation liquor obtained in the step (7) according to the volume ratio of 1:1Uniformly mixing to obtain the compound microbial deodorant; the total number of viable bacteria in the obtained composite microbial deodorant is 3.6 × 1010cfu/ml/ml。
Example 3
Application of composite microbial deodorant
Diluting the compound microorganism deodorant of example 1 or example 2 by 20 times with water, spraying into air with odor by spraying equipment, wherein the spraying height is not less than 2m, and the dosage is 0.05L/m2。
Example 4
Application of composite microbial deodorant
Diluting the compound microbial deodorant of example 1 or example 2 by 10 times with water, and uniformly spraying the diluted compound microbial deodorant on the surface of solid waste garbage by using a spraying device, wherein the dosage is 0.5L/m2。
The compound microbial deodorant is tried in the Beijing four-season green organic resource regeneration center, and the results are shown in table 1:
TABLE 1 results of the deodorizing test of composite microbial deodorant
Figure GDA0002626097880000141
Test results show that the composite microbial deodorant has good deodorization effect on air and solid waste garbage, and is long in duration.
The embodiment is only a part of the invention, and the equivalent modifications made by the person skilled in the art are all within the protection scope of the invention.

Claims (10)

1. A preparation method of a compound microbial deodorant is characterized by comprising the following steps: it includes: the method comprises the following steps:
preparation of environment-friendly enzyme
Preparing 9kg of brown sugar or molasses, 1kg of yeast extract, 20-30kg of fruit or fruit peel and 100L of purified water; dissolving brown sugar or molasses and yeast extract in boiling water at a temperature of not higher than 40 ℃ to form sugar water, cleaning fruits or fruit peels with tap water, cleaning a ferment fermentation tank, and rubbing with 60-80% ethanol for fermentA fermenter; then sequentially adding fruits or fruit peels, the sugar water and purified water, and sealing the fermentation tank for natural fermentation for 30-100 days; the total number of viable bacteria in the obtained environment-friendly enzyme solution is 3.0 multiplied by 1010cfu/ml— 5.0×1010cfu/ml, and the PH value is 3.5-4;
(2) preparing photosynthetic bacteria seed liquid:
(a) culture of photosynthetic bacteria in slant culture medium
Sequentially adding 3.5g of sodium acetate, 1g of ammonium chloride, 0.1g of magnesium chloride, 0.1g of calcium chloride, 0.4g of monopotassium phosphate, 0.6g of dipotassium hydrogen phosphate, 0.1g of yeast extract and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, then adding the purified water to the constant volume of 1000mL, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then photosynthetic bacteria strains are purchased and placed into the bottle to be shaken uniformly, then the photosynthetic bacteria strains are inoculated on the slant culture medium by using an inoculating loop in a high density manner, and the activated photosynthetic bacteria strains are obtained after activation for 18-24 hours at the temperature of 30-33 ℃;
(b) preparation of photosynthetic bacteria in a liquid culture medium:
adding 3g of sodium acetate, 2g of ammonium chloride, 0.1g of sodium chloride, 0.05g of calcium chloride, 0.5g of monopotassium phosphate, 0.3g of dipotassium phosphate, 0.2g of magnesium sulfate, 0.02g of manganese sulfate, 0.002g of ferric trichloride and 0.1g of yeast extract into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated photosynthetic bacteria strain in the step (a) into the liquid culture medium in the step (b) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaker at the temperature of 34-38 ℃ for 40-50 h at the speed of 100-150 r/min, then the optical density of each bacterial suspension OD600 is detected, and when the optical density of each bacterial suspension OD600 is equal to that of each bacterial suspensionStopping culturing at or above 4.0, wherein viable bacteria count is 3.0 × 108cfu/ml～4.0×108cfu/ml photosynthetic bacteria seed solution;
(3) preparation of yeast seed liquid
(c) Preparation of slant culture medium for yeast
Adding 20g of glucose, 10g of yeast extract, 20g of peptone and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then yeast strains are purchased and placed into the bottle to be shaken up, then the yeast strains are inoculated on the slant culture medium by an inoculating loop in high density and activated for 18-24 h at the temperature of 30-33 ℃, and the activated yeast strains are obtained;
(d) preparation of yeast in liquid culture medium
Adding 9g of glucose, 45g of molasses, 34-38 g of bran, corn DDGS3g, 2.75g of cottonseed meal, 0.75g of yeast extract, 0.8g of ammonium sulfate, 3g of monopotassium phosphate, 0.3g of magnesium sulfate and 0.3g of calcium chloride into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated saccharomycete strain in the step (c) to the liquid culture medium in the step (d) for culture, wherein the cultured liquid is called bacterial suspension, culturing the bacterial suspension in a shaker at the temperature of 34-38 ℃ for 40-50 h at the speed of 100-150 r/min, detecting the optical density of each bacterial suspension OD600, stopping the culture when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, and obtaining the viable bacteria with the viable bacteria number of 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of yeast seed solution;
(4) preparation of lactic acid bacteria seed liquid
(e) Preparation of lactic acid bacteria in a slant culture medium:
sequentially adding 5g of peptone, 2g of corn steep liquor, 40g of sucrose, 5g of sodium acetate, 10g of sodium chloride, 5g of magnesium sulfate and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 20g of agar, fully stirring for dissolving the agar, fixing the volume to 1000mL by using the purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing lactobacillus strains, putting the lactobacillus strains into the bottle, shaking the bottle uniformly, respectively inoculating the lactobacillus strains on the slant culture medium with high density by using inoculating loops, and activating for 18 to 24 hours at the temperature of 30 to 33 ℃ to obtain activated lactobacillus strains;
(f) preparation of lactic acid bacteria in liquid culture medium
Sequentially adding 10g of peptone, 8g of beef extract, 4g of yeast extract, 2g of diammonium hydrogen citrate, 20g of glucose, 801g of tween, 5g of sodium acetate, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate and 0.04g of manganese sulfate into a 3L beaker, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.0 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated lactobacillus strain in the step (e) into the liquid culture medium in the step (f) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 100-150 r/min for 40-50 h at the temperature of 34-38 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of lactobacillus seed solution;
(5) preparation of Actinomycetes seed liquid
(g) Preparing an actinomycete slant culture medium:
adding 3g of beef extract, 10g of peptone, 5g of sodium chloride and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 15g of agar, dissolving with 1000mL of purified water, and adjusting the pH value to 7.2 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of sterile water by using a sterile micropipette, putting the sterile water into a bottle, purchasing actinomycete strains, putting the actinomycete strains into the bottle, shaking the actinomycete strains uniformly, inoculating the actinomycete strains on the slant culture medium by using an inoculating loop in a high-density manner, and activating the actinomycete strains for 18 to 24 hours at the temperature of between 30 and 33 ℃ to obtain activated actinomycete strains;
(h) preparation of actinomycete liquid culture medium:
adding 10g of glucose, 4g of yeast extract, 4g of peptone, 4g of dipotassium hydrogen phosphate, 2g of potassium dihydrogen phosphate and 0.5g of magnesium sulfate into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated actinomycete strain in the step (g) to a liquid culture medium in the step (h) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaker at the temperature of 34-38 ℃ for 40-50 h at the speed of 100-150 r/min, then the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 10 at the moment8cfu/ml～4.0×108cfu/ml actinomycete seed solution;
(5) preparation of Bacillus seed liquid
(i) Preparation of a bacillus slant culture medium:
sequentially adding 10g of peptone, 15g of beef extract, 15g of sodium chloride and 800mL of purified water into a 3L beaker, stirring for dissolving, boiling, adding 18g of agar, diluting to 1000mL with purified water, and adjusting the pH value to 7 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under aseptic operation, sucking 50 mu L of aseptic water by using an aseptic micropipette, putting the aseptic water into a bottle, purchasing bacillus strains, putting the bacillus strains into the bottle, shaking the bacillus strains uniformly, inoculating the bacillus strains on the slant culture medium at high density by using an inoculating loop, and activating for 18-24 h at the temperature of 30-33 ℃ to obtain activated bacillus strains;
(j) preparation of a bacillus liquid culture medium:
adding 20g of corn starch, 10g of corn steep liquor, 5g of ammonium sulfate, 2g of monopotassium phosphate, 1g of magnesium sulfate and 1g of sodium chloride into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated bacillus strain in the step (i) into the liquid culture medium in the step (j) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaking table of 100-150 r/min for 40-50 h at the temperature of 34-38 ℃, the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml of bacillus seed solution;
(6) preparation of acetic acid bacteria seed liquid
(k) Preparation of acetic acid bacteria slant culture medium: adding 10g of glucose, 10g of yeast powder, 10g of calcium carbonate and 800mL of purified water into a 3L beaker in sequence, stirring for dissolving, boiling, adding 25g of agar, adding purified water to reach the constant volume of 1000mL, and adjusting the pH value to 6.5 +/-0.2; subpackaging the prepared culture medium into 50ml test tubes, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure for 12-16 min at the temperature of 115-125 ℃; after sterilization, the head of the test tube is rested on a batten, so that the culture medium in the tube is naturally inclined, and the inclined culture medium is obtained after solidification; under the aseptic operation, a sterile micropipette is used for sucking 50 mu L of sterile water and placing the sterile water into a bottle, then acetic acid bacteria strains are purchased and placed into the bottle to be shaken uniformly, then inoculating the acetic acid bacteria strains on the slant culture medium with an inoculating loop in a high density, and activating for 18-24 h at the temperature of 30-33 ℃ to obtain activated acetic acid bacteria strains;
(l) Preparation of acetic acid bacteria liquid culture medium:
adding 10g of glucose, 10g of yeast powder and 10mL of edible alcohol into a 3L beaker in sequence, dissolving with purified water, fixing the volume to 1000mL, and adjusting the pH value to 5.5 +/-0.2; subpackaging the prepared liquid culture medium into 250ml conical flasks, sealing, putting into a high-pressure steam sterilization pot, and carrying out high pressure 12-16 min at the temperature of 115-125 ℃; inoculating the activated acetic acid bacteria strain in the step (k) into the liquid culture medium in the step (l) for culture, wherein the cultured liquid is called bacterial suspension, the bacterial suspension is cultured in a shaker at the temperature of 34-38 ℃ at the speed of 100-150 r/min for 40-50 h, then the optical density of each bacterial suspension OD600 is detected, when the optical density of each bacterial suspension OD600 is equal to or more than 4.0, the culture is stopped, and the number of the obtained viable bacteria is 3.0 multiplied by 108cfu/ml～4.0×108cfu/ml acetic acid bacteria seed liquid;
(7) preparation of Mixed microbial fermentation broth
Dissolving 1kg of brown sugar in 9L of distilled water to obtain a brown sugar culture medium; respectively taking 20-40% of photosynthetic bacteria seed liquid, 10-30% of saccharomycete seed liquid, 5-15% of lactic acid bacteria seed liquid, 5-15% of actinomycete seed liquid, 5-15% of bacillus seed liquid and 5-15% of acetic acid bacteria seed liquid in the steps (2) to (6) to mix into a composite microorganism, inoculating the composite microorganism into the glucose culture medium, and carrying out sealed fermentation at the temperature of 20-25 ℃ for 7-10 days to obtain the active bacteria with the number of 3.0 multiplied by 1010cfu/ml～4.0×1010cfu/ml of mixed microbial fermentation broth;
(8) mixing of environmental-friendly enzyme and microbial fermentation broth
Filtering the environment-friendly enzyme obtained in the step (1), and uniformly mixing the environment-friendly enzyme and the mixed microorganism fermentation liquor obtained in the step (7) according to the volume ratio of 0.5-1: 1-1.5 to obtain a composite microorganism deodorant; the total number of viable bacteria in the obtained composite microbial deodorant is 3.5 × 1010cfu/ml/ml～4.5×1010cfu/ml。
2. The method for preparing a composite microbial deodorant according to claim 1, characterized in that: the fruit or the peel is one or more of apple, pear, peach, pineapple, sugarcane, orange, watermelon, melon or the peel, and the purified water is distilled water.
3. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the photosynthetic strain is Rhodopseudomonas palustris (Rhodopseudomonas palustris) which is researched and preserved by institute of microbiology of Chinese academy of sciences, the strain source is Chinese, and the preservation number is as follows: CGMCC No.1.8929, and the preservation date is 2014, 12 months and 16 days.
4. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the yeast strain is Rhodotorula mucilaginosa (Rhodotorula mucor) preserved by China general microbiological culture preservation management center, the strain source is China, and the preservation number is as follows: CGMCC No.2.4008, preservation date of 2010, 7 months and 13 days.
5. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the Lactobacillus strain is acidophilic Lactobacillus (Lactobacillus acidophilus) preserved by the China agricultural microbial strain preservation management center, the strain source is China, and the preservation number is as follows: ACCC10637, with a preservation date of 2013, 10 months and 14 days.
6. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the actinomycete is rumen-inhabiting actinomycete (Actinomyces ruminicola) preserved by China general microbiological culture preservation management center, the actinomycete comes from China, and the preservation number is as follows: CGMCC1.5030, with preservation date of 6/3/2005.
7. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the Bacillus species is Bacillus thuringiensis (Bacillus thuringiensis) preserved by China general microbiological culture Collection center, the strain source is China, and the preservation number is as follows: CGMCC 1.4671, with preservation date of 11 months and 1 day in 2013.
8. The method for preparing a composite microbial deodorant according to claim 2, characterized in that: the acetic acid strain is Acetobacter pasteurianus (Acetobacter pasteurianus) preserved by China center for industrial microorganism strain preservation management, the strain source is China, and the preservation number is as follows: SICC 1.3, with a date of storage of 2008, 11 months and 15 days.
9. A composite microbial deodorant produced by the method for producing a composite microbial deodorant according to any one of claims 1 to 8.
10. The method of using a composite microbial deodorant according to claim 9, wherein: the composite microbial deodorant is diluted by 10-20 times with water, and is sprayed in the air with odor by spraying equipment, wherein the spraying height is not less than 2m, and the using amount is 0.05L-0.5L/m2。
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