CN108310117A - A method of extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex - Google Patents
A method of extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex Download PDFInfo
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- CN108310117A CN108310117A CN201810245443.5A CN201810245443A CN108310117A CN 108310117 A CN108310117 A CN 108310117A CN 201810245443 A CN201810245443 A CN 201810245443A CN 108310117 A CN108310117 A CN 108310117A
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- cortex
- method described
- matrimony vine
- lycii radicis
- branch skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The method that the present invention provides a kind of to extract flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex.This method utilizes the fermentation of saccharomycete, generate a variety of active ingredients such as cellulase, pectase, the ingredients such as the fully degraded lycii radicis,cortex and cellulose of branch skin, lignin and pectin, dismiss the structure of original closely iuntercellular and cell wall, to be effectively increased the dissolution rate of total flavonoids of lycium barbarum L, the extraction efficiency of total flavonoids of lycium barbarum L is improved.
Description
Technical field
The method that the present invention relates to a kind of to extract flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex.
Background technology
Matrimony vine or lycium barbarum are a kind of common help class Chinese medicine, and there are many medicinal part, include mainly fruit（Matrimony vine
Son）, root skin（The root bark of Chinese wolf-berry）, wolfberry leaf（Its essence grass）And the parts such as branch skin.The root skin of matrimony vine mainly contains Anthraquinones with stem skin, fragrant
The active ingredients such as legumin class, flavonoids and organic acid, for hypoglycemic, lowering blood pressure and blood fat, antipyretic, antibacterial and antiviral etc.
Aspect.After testing in lycii radicis,cortex and stem skin the main Types of flavones be 4',5,7-trihydroxyflavone, acacetin, cyanidenon, Quercetin and
Linarin has higher medical treatment and health value.The general flavone content of wherein wild barbarum branch skin is about 8.932mg/g, only secondary
In the general flavone content of lycii radicis,cortex（9-10mg/g), and the content of general flavone reaches in the plant barbarum root skin of Qaidam
14.5mg/g, and increase with the increase of growth time, thus there is higher Development volue.In medlar cultivation area, every year
It will prune to matrimony vine, also have a large amount of matrimony vine plant and pulled out because of aging, these roots and branch skin are efficiently used
With good ecology and economic value.
Most general flavones are present in cell inclusion, and extracting method generally utilizes the good penetrability of alcohol, are dissolved out thin
It is extracellular.But there are a large amount of bast fiber in matrimony vine branch skin and the root bark of Chinese wolf-berry, cell wall is also by lignin, cellulose and pectin
Equal ingredients are constituted, they can all influence the dissolution rate of total flavonoids of lycium barbarum L, and then reduce the extraction efficiency of total flavonoids of lycium barbarum L.
Invention content
Based on mulberry tree problem, so this method utilizes the fermentation of saccharomycete, it is a variety of to generate cellulase, pectase etc.
The ingredients such as cellulose, lignin and the pectin of active constituent, fully degraded lycii radicis,cortex and branch skin dismiss original close cell
Between and the structure of cell wall improve the extraction efficiency of total flavonoids of lycium barbarum L to be effectively increased the dissolution rate of total flavonoids of lycium barbarum L.This hair
It is bright to provide a kind of method for extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex.Specifically, it includes following behaviour
Make：
（1）Matrimony vine branch skin or/and lycii radicis,cortex, water are taken, is crushed, the slurries after must crushing；
（2）Slurries are taken, are sterilized, inoculation Fibrolytic bacteria, saccharomycete ah109 bacterial strains or saccharomycete y187 bacterial strains, fermentation wait fermenting
It finishes, collects solid content；
（3）The mixed solvent of one or more of solid content methanol, ethyl alcohol, water extracts, you can obtains containing flavones
The extract of constituents.
Further, step（1）In, the equipment of crushing is selected from colloid mill.
Wherein, the Homogenization time of colloid mill is at least 20min；It is further selected from 25 ~ 40min.
Wherein, step（1）In, matrimony vine branch skin or/and the mass ratio of lycii radicis,cortex and water are 1：5 ~ 10, it is further selected from 1：
6。
Wherein, step（2）In, fermentation temperature is 25 ~ 30 DEG C, is further selected from 28 ± 1 DEG C.
Wherein, step（2）In, fermentation time is at least 24 hours, is further selected from 36 ~ 72 hours.
Wherein, step（2）In, it is 5% that Fibrolytic bacteria, saccharomycete ah109 bacterial strains or saccharomycete y187 bacterial strains, which connect bacterium amount,.
Wherein, step（3）60 ~ 85% ethyl alcohol of middle selection are solvent, are further selected from 70 ~ 80%.
Wherein, step（3）The dosage of middle solvent is step（1）5 ~ 10 times of matrimony vine branch skin or/and lycii radicis,cortex weight.
Wherein, step（3）In, using refluxing extraction；Further, extraction time is not less than 60min;Further,
Extraction time is selected from 100 ~ 140min.
The present invention carries out fermentation process by introducing lactic acid bacteria, effectively increases yellow in matrimony vine branch skin or/and lycii radicis,cortex
The dissolution and extraction of ketones component, effectively increase the utilization rate of resource.
Specific implementation mode
With reference to specific embodiment, the present invention is further elaborated, but the present invention does not limit to and following embodiment,
The raw material can obtain unless otherwise specified from open commercial sources.
Bacterial strain uses therefor of the present invention is selected from Fibrolytic bacteria, saccharomycete ah109 bacterial strains or saccharomycete y187 bacterial strains；Wherein, fine
It ties up degradation bacteria and is purchased from Guangzhou infinitesimal bio tech ltd.
Embodiment 1
1, it selects the 7 years raw plant barbarums in Chaidamu of Qinghai Province as raw material, washes with water clean root and the silt on stem surface.Stripping
The skin of root and stem is taken, 60 DEG C drying to constant weight.
2,10 kilograms of above-mentioned lycii radicis,cortex and stem skin are weighed respectively, respectively the purified water of 60 kilograms of addition.It is put in respectively
In colloid mill, it is homogenized half an hour.
3, the homogenate of stem skin and root skin is collected respectively, and sterilize 30min in 121 DEG C of high-pressure sterilizing pots.
4, after root skin and the cooling of branch skin slurries, yeast strain is respectively connected to by 5% bacterium amount that connects.
5, the homogenate after sterilizing is placed in fermentation tank, opens stirring, 28 DEG C, ferments 48 hours.
6, homogenate is filtered, precipitation is collected.
7,60 kilograms of addition, 75% alcohol, refluxing extraction 120 minutes in precipitating.
8, extracting solution is collected, total flavonoids of lycium barbarum L powder is obtained after concentrate drying.It weighs respectively and detects flavones content.
Experimental result：Wherein total flavonoids of lycium barbarum L is obtained in lycii radicis,cortex（Rutin meter）Extract 245.3g, content are
26.5%, the amount for obtaining 100% flavones is 65.0045g
Matrimony vine branch skin obtains total flavonoids of lycium barbarum L（Rutin meter）Extract 210g, content 22.4%, 100% obtained flavones amount are
47.04g
Contrast test：
1, it selects the 7 years raw plant barbarums in Chaidamu of Qinghai Province as raw material, washes with water clean root and the silt on stem surface.
The skin of root and stem is stripped, 60 DEG C drying to constant weight.
2,10 kilograms of above-mentioned lycii radicis,cortex and branch skin are weighed respectively, respectively the purified water of 60 kilograms of addition.It is put in respectively
In tissue refiner, it is homogenized half an hour.
3, homogenate is filtered, precipitation is collected.
4,60 kilograms of addition, 75% alcohol, refluxing extraction 120 minutes in precipitating.
5, extracting solution is collected, total flavonoids of lycium barbarum L powder is obtained after concentrate drying.It weighs and detects flavones content.
Experimental result：Total flavonoids of lycium barbarum L is obtained in lycii radicis,cortex（Rutin meter）Extract 198.6g, content 27.4%, folding
Flavones weight after pure is 54.4164g
Matrimony vine branch skin obtains total flavonoids of lycium barbarum L（Rutin meter）Extract 178.1g, content 23.5%, it is pure after flavones weight be
41.8535g
Saccharomycete can eccrine fiber element enzyme, pectase and other enzymes, the various fibers for the matrimony vine bast that can effectively degrade,
The ingredients such as pectin.Promote original compact arranged fiber, the structures such as cell wall to become loose, to be conducive to total flavonoids of lycium barbarum L from
Intracellular effective dissolution.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
Protection scope of the present invention is also should be regarded as into retouching.
Claims (10)
1. a kind of method for extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex, it is characterised in that：It includes as follows
Operation：
（1）Matrimony vine branch skin or/and lycii radicis,cortex, water are taken, is crushed, the slurries after must crushing；
（2）Slurries are taken, are sterilized, inoculation Fibrolytic bacteria, saccharomycete ah109 bacterial strains or saccharomycete y187 bacterial strains（Purchased from Guangzhou
Infinitesimal bio tech ltd）, fermentation, waiting fermenting finishes, and collects solid content；
（3）One or two kinds of mixed solvent extractions of chromocor extract methanol, ethyl alcohol or water, you can obtain containing flavonoids
The extract of ingredient.
2. according to the method described in claim 1, it is characterized in that：Step（1）In, the equipment of crushing is selected from colloid mill.
3. according to the method described in claim 2, it is characterized in that：The Homogenization time of colloid mill is at least 20min-60min；Into
One step is selected from 25 ~ 40min.
4. according to the method described in claim 1, it is characterized in that：Step（1）In, matrimony vine branch skin or/and lycii radicis,cortex and water
Mass ratio be 1：5 ~ 10, it is further selected from 1：6.
5. according to the method described in claim 1, it is characterized in that：Step（2）In, fermentation temperature is 25 ~ 30 DEG C, is further selected
From 28 ± 1 DEG C.
6. according to the method described in claim 1, it is characterized in that：Step（2）In, fermentation time is at least 24 hours -72 small
When, it is further selected from 48 ~ 60 hours.
7. according to the method described in claim 1, it is characterized in that：Step（2）In, Fibrolytic bacteria, saccharomycete ah109 bacterial strains
Or it is 3-10% that saccharomycete y187 bacterial strains, which account for the ratio of slurries, is further selected from 5%.
8. according to the method described in claim 1, it is characterized in that：Step（3）60 ~ 85% ethyl alcohol of middle selection are solvent, further
Selected from 70 ~ 80%.
9. according to the method described in claim 1, it is characterized in that：Step（3）The dosage of middle solvent is step（1）Matrimony vine branch skin
Or/and 5 ~ 10 times of lycii radicis,cortex weight.
10. according to the method described in claim 1, it is characterized in that：Step（3）In, using refluxing extraction；Further, it carries
The time is taken to be not less than 60min;Further, extraction time is selected from 100 ~ 140min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810245443.5A CN108310117A (en) | 2018-03-23 | 2018-03-23 | A method of extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex |
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| CN201810245443.5A CN108310117A (en) | 2018-03-23 | 2018-03-23 | A method of extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex |
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| CN108310117A true CN108310117A (en) | 2018-07-24 |
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| CN201810245443.5A Pending CN108310117A (en) | 2018-03-23 | 2018-03-23 | A method of extracting flavones ingredient from matrimony vine branch skin or/and lycii radicis,cortex |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997954A (en) * | 2019-04-09 | 2019-07-12 | 宁夏农林科学院枸杞工程技术研究所 | A kind of technique preparing biological feedstuff using fructus lycii branch |
Citations (2)
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|---|---|---|---|---|
| CN104083525A (en) * | 2014-06-16 | 2014-10-08 | 南京泽朗医药科技有限公司 | Method for extraction of cortex lycii total flavonoids from cortex lycii |
| CN104225073A (en) * | 2014-09-05 | 2014-12-24 | 南京泽朗生物科技有限公司 | Preparation method and application of cortex lycii radicis total flavone |
-
2018
- 2018-03-23 CN CN201810245443.5A patent/CN108310117A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104083525A (en) * | 2014-06-16 | 2014-10-08 | 南京泽朗医药科技有限公司 | Method for extraction of cortex lycii total flavonoids from cortex lycii |
| CN104225073A (en) * | 2014-09-05 | 2014-12-24 | 南京泽朗生物科技有限公司 | Preparation method and application of cortex lycii radicis total flavone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997954A (en) * | 2019-04-09 | 2019-07-12 | 宁夏农林科学院枸杞工程技术研究所 | A kind of technique preparing biological feedstuff using fructus lycii branch |
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Application publication date: 20180724 |