CN108298847B - Method for consolidating desert sand by biological magnesium ammonium phosphate cement and method for testing performance of biological sandstone formed by solidification - Google Patents
Method for consolidating desert sand by biological magnesium ammonium phosphate cement and method for testing performance of biological sandstone formed by solidification Download PDFInfo
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- 239000004576 sand Substances 0.000 title claims abstract description 53
- 239000004568 cement Substances 0.000 title claims abstract description 39
- MXZRMHIULZDAKC-UHFFFAOYSA-L ammonium magnesium phosphate Chemical compound [NH4+].[Mg+2].[O-]P([O-])([O-])=O MXZRMHIULZDAKC-UHFFFAOYSA-L 0.000 title claims abstract description 31
- 229910052567 struvite Inorganic materials 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000012360 testing method Methods 0.000 title claims description 8
- 238000007711 solidification Methods 0.000 title abstract description 6
- 230000008023 solidification Effects 0.000 title abstract description 6
- 239000000243 solution Substances 0.000 claims abstract description 36
- 238000005507 spraying Methods 0.000 claims abstract description 24
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 12
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004202 carbamide Substances 0.000 claims abstract description 10
- 235000015097 nutrients Nutrition 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 8
- 230000003628 erosive effect Effects 0.000 claims abstract description 8
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 241000193395 Sporosarcina pasteurii Species 0.000 claims abstract description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910001629 magnesium chloride Inorganic materials 0.000 claims abstract description 6
- 239000005018 casein Substances 0.000 claims abstract description 5
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000021240 caseins Nutrition 0.000 claims abstract description 5
- 244000068988 Glycine max Species 0.000 claims abstract description 4
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 4
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- 241000209082 Lolium Species 0.000 claims description 20
- 230000000813 microbial effect Effects 0.000 claims description 10
- 238000007664 blowing Methods 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 238000002591 computed tomography Methods 0.000 claims description 5
- 230000007547 defect Effects 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- 239000010410 layer Substances 0.000 claims description 4
- 239000002344 surface layer Substances 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052782 aluminium Inorganic materials 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 238000011160 research Methods 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 238000011056 performance test Methods 0.000 abstract description 6
- 239000000428 dust Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 abstract description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 41
- 238000001723 curing Methods 0.000 description 13
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 238000001878 scanning electron micrograph Methods 0.000 description 4
- 229910017958 MgNH Inorganic materials 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000606860 Pasteurella Species 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- JWSMTBMIGYJJJM-UHFFFAOYSA-N magnesium;azane Chemical compound N.[Mg+2] JWSMTBMIGYJJJM-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- TUSGNPMWMZKAGK-UHFFFAOYSA-H trimagnesium;diphosphate;hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O TUSGNPMWMZKAGK-UHFFFAOYSA-H 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B12/00—Cements not provided for in groups C04B7/00 - C04B11/00
- C04B12/02—Phosphate cements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
- G01N15/088—Investigating volume, surface area, size or distribution of pores; Porosimetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a method for consolidating desert sand by biological magnesium ammonium phosphate cement, which comprises the following steps: A. firstly, preparing a nutrient solution, wherein the pH =7, and the nutrient solution is a mixed solution of 15g/L casein and 5g/L soyabean peptone; then selecting and culturing Bacillus sporogenes (Sporosarcina-Pasteurii); B. adding a mixed solution of MgCl2 & 2H2O and urea into the culture solution of the spore pasteur bacillus according to 3mol/L, and adding K2HPO4 & 3H2O according to 3mol/L to form a biological solidification solution; C. spraying the biological curing solution with a unit area spraying amount of 3L/m2The biological sandstone is circularly sprayed on the desert sand, and the sprayed desert sand is stored in the air for 3 days to form the biological sandstone. The invention also discloses a performance test method of the biological sandstone cured by the method for consolidating the desert sand by using the biological magnesium ammonium phosphate cement. The biological solidification solution prepared by the method provided by the invention is sprayed on desert sand to form biological sandstone, so that the wind erosion rate can be greatly reduced, and sand dust generated in desert areas can be effectively inhibited.
Description
Technical Field
The invention relates to a method for consolidating desert sand by biological magnesium ammonium phosphate cement. The invention also relates to a performance test method of the solidified biological sandstone.
Background
Desertification and sand storm are great hazards in northwest China, and the traditional treatment method mainly adopts a mode of afforestation to solidify land and block sand and dust, but the method needs long-term construction to produce effects, and is more ideal if some short-term means can be combined to quickly inhibit sand and dust and solidify sand and soil for a long time.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for consolidating desert sand by biological magnesium ammonium phosphate cement, which can more environment-friendly and more quickly solidify sandy soil. The technical problem to be solved by the invention is also to provide a performance test method of the biological sandstone cured and formed by the method for consolidating the desert sand by using the biological magnesium ammonium phosphate cement.
Therefore, the method for consolidating desert sand by using the biological magnesium ammonium phosphate cement provided by the invention is characterized by comprising the following steps of: the method comprises the following steps:
A. firstly, preparing a nutrient solution, wherein the pH =7, and the nutrient solution is a mixed solution of 15g/L casein and 5g/L soyabean peptone; then selecting and culturing the spore bacillus Sporosarcina-Pasteurii; measuring the OD value of the sample in the range of 1.5-1.9 by an ultraviolet spectrophotometer;
B. MgCl is added into the culture solution of spore pasteur bacillus according to 3mol/L2·2H2Adding K into the mixed solution of O and urea according to the proportion of 3mol/L2HPO4·3H2O forms a biological curing solution, namely biological magnesium ammonium phosphate cement slurry; in the formulation K2HPO4·3H2O and MgCl2·2H2The molar ratio of O to urea is 1: 1: 1;
C. spraying the biological curing solution with the unit area of 3L/m2The biological sandstone is formed after the desert sand is stored in the air for 3 days after being sprayed, the storage temperature is required to be 5-45 ℃, and the two end point values of the storage temperature fluctuate within the range of 0.5 ℃.
The invention also provides a performance test method of the biological sandstone cured and formed by the method for consolidating the desert sand by using the biological magnesium ammonium phosphate cement, which comprises the following steps:
A. and (3) testing the rheumatism rate: firstly, spraying the biological curing solution on the surface of the desert for 1 time, taking out the biological sandstone, and weighing to obtain a quality value, and spraying the biological curing solution on the surface of the desert for 3 times, taking out the biological sandstone, and weighing to obtain a quality value; then wrapping the bottoms and the peripheries of the two biological sandstone samples which are generated by spraying by adopting aluminum sheets, only exposing the upper parts of the biological sandstone, and then placing the biological sandstone into an inner hole of an air duct, wherein the air duct comprises a barrel body, a fan is fixed at one end of the barrel body, a sample fixing groove is arranged in the barrel body, the sample fixing groove is formed by surrounding boards, and the two biological sandstone samples which are generated by spraying are uniformly placed into the fixing groove; then starting a fan to control the average wind speed to be 12.0m/s, and continuously blowing for 1 hour; finally, calculating to obtain the wind erosion rates of the two biological sandstones which are respectively sprayed for 1 time and 3 times and are respectively 0g/m by weighing the mass value of the biological sandstones before blowing and subtracting the mass value after blowing2H and 0g/m2/h；
B. Spraying the biological curing solution on the surface of the desert for 3 times and taking out a biological sandstone sample; the three-dimensional pore structure evolution of the biological sandstone sample researches the cementation of the biological magnesium ammonium phosphate in the sandstone by an X-ray computed tomography technology, and the maximum defect volume of the biological sandstone sprayed for 3 times is 134mm3The XRCT determined average porosity of the biological sandstone is 18.39%, and the result further proves that the biological magnesium ammonium phosphate cement can well fill the porosity among the desert sand grains;
C. circularly spraying the biological curing solution on the surface layer of the desert sand for 3 times and taking a biological sandstone sample; and (2) loading the biological sandstone sample into a container, planting ryegrass into the biological sandstone sample when a sand grain solidified layer is damaged, wherein the ryegrass can successfully grow in a blank group after 4 days, and can also grow in the biological sandstone sample after 11 days, the ryegrass can delay growth in the microbial cement group because the sand environment of the microbial cement group is weakly alkaline, and the growth rate of the ryegrass is consistent with that of the blank group after the ryegrass grows in the blank group and the biological sandstone sample group for 15 days.
The invention provides the following technical effects: 1. the biological magnesium ammonium phosphate cement is adopted to cure the desert sand, and because a biological curing method is adopted, the environmental pollution is less, and the later period of tree planting cannot be influenced; 2. the biological solidification solution can be quickly solidified in sand to form biological sandstone, so that the sand uplift is inhibited; 3. the performance test method for the biological sandstone is simple and effective, and can quickly obtain various data such as wind erosion rate and the like.
Drawings
FIG. 1 is an X-ray diffraction pattern of desert sand (a) and biological sandstone (b);
FIG. 2 is an SEM image of desert sand;
fig. 3 is an SEM image of biological sandstone;
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are provided for illustration only and are not intended to limit the invention.
The method for consolidating desert sand by using the biological magnesium ammonium phosphate cement provided by the invention comprises the following steps:
A. firstly, preparing a nutrient solution, wherein the pH =7, and the nutrient solution is a mixed solution of 15g/L casein and 5g/L soyabean peptone; then selecting and culturing the spore bacillus Sporosarcina-Pasteurii; measuring the OD value of the sample in the range of 1.5-1.9 by an ultraviolet spectrophotometer;
B. MgCl is added into the culture solution of spore pasteur bacillus according to 3mol/L2·2H2Adding K into the mixed solution of O and urea according to the proportion of 3mol/L2HPO4·3H2O forms a biological curing solution, namely biological magnesium ammonium phosphate cement slurry; in the formulation K2HPO4·3H2O and MgCl2·2H2The molar ratio of O to urea is 1: 1: 1;
C. spraying the biological curing solution with the unit area of 3L/m2The biological sandstone is formed after the desert sand is stored in the air for 3 days after being sprayed, the storage temperature is required to be 5-45 ℃, and the two end point values of the storage temperature fluctuate within the range of 0.5 ℃.
The invention also provides a performance test method of the biological sandstone cured and formed by the method for consolidating the desert sand by using the biological magnesium ammonium phosphate cement, which comprises the following steps:
A. and (3) testing the rheumatism rate: firstly, spraying the biological curing solution on the surface of the desert for 1 time, taking out the biological sandstone, and weighing to obtain a quality value, and spraying the biological curing solution on the surface of the desert for 3 times, taking out the biological sandstone, and weighing to obtain a quality value; then wrapping the bottoms and the peripheries of the two biological sandstone samples which are generated by spraying by adopting aluminum sheets, only exposing the upper parts of the biological sandstone, and then placing the biological sandstone into an inner hole of an air duct, wherein the air duct comprises a barrel body, a fan is fixed at one end of the barrel body, a sample fixing groove is arranged in the barrel body, the sample fixing groove is formed by surrounding boards, and the two biological sandstone samples which are generated by spraying are uniformly placed into the fixing groove; then starting a fan to control the average wind speed to be 12.0m/s, and continuously blowing for 1 hour; and finally, calculating to obtain the wind erosion rates of the two biological sandstones after being sprayed for 1 time and 3 times by subtracting the mass value after the air blowing from the mass value before the air blowing of the two biological sandstonesAre respectively 0g/m2H and 0g/m2/h；
B. Spraying the biological curing solution on the surface of the desert for 3 times and taking out a biological sandstone sample; the three-dimensional pore structure evolution of the biological sandstone sample researches the cementation of the biological magnesium ammonium phosphate in the sandstone by an X-ray computed tomography technology, and the maximum defect volume of the biological sandstone sprayed for 3 times is 134mm3The XRCT determined average porosity of the biological sandstone is 18.39%, and the result further proves that the biological magnesium ammonium phosphate cement can well fill the porosity among the desert sand grains;
C. circularly spraying the biological curing solution on the surface layer of the desert sand for 3 times and taking a biological sandstone sample; and (2) loading the biological sandstone sample into a container, planting ryegrass into the biological sandstone sample when a sand grain solidified layer is damaged, wherein the ryegrass can successfully grow in a blank group after 4 days, and can also grow in the biological sandstone sample after 11 days, the ryegrass can delay growth in the microbial cement group because the sand environment of the microbial cement group is weakly alkaline, and the growth rate of the ryegrass is consistent with that of the blank group after the ryegrass grows in the blank group and the biological sandstone sample group for 15 days.
The technical solution of the present invention is explained in detail below in the form of examples:
the Pasteurella sporogenes Sporosarcina-Pasteurii was selected by a specific nutrient solution (pH =7, mixed solution of casein 15g/L and soytone 5 g/L) and cultured. K2HPO4Purchased from the national pharmaceutical group chemical agents limited. Preparation of MgCl before use2·2H2A mixed solution of O and urea (3 mol/L). Desert sand is obtained from Dunhuang, China.
Mixing MgCl2·2H2O and urea (3 mol/L) and K2HPO4·3H2The mixed solution of Sporosarcina-Pasteurii of O (2 mol/L) is sprayed circularly, and finally, all the biological sandstones are kept in the air for 3 days (30 +/-2 ℃).
The chemical composition of desert sand and biological sandstone was examined by X-ray diffraction (λ = 1.5406). The forms and the element compositions of the desert and the biological sandstone are respectively measured by a Scanning Electron Microscope (SEM) and a Genesis 60S energy dispersion X-ray spectrometer (EDS). The hardness of biological sandstones was tested by shore durometer. According to literature reports, 3D images of biological sandstone were measured by X-ray computed tomography (XRCT).
Performance testing
XRD confirmed that the chemical composition of the desert was quartz sand (JCPDS card number 85-0504) (see desert sand a in FIG. 3). The biological sandstone is prepared from magnesium ammonium phosphate- [ MgNH ]4PO4（H2O) 6 (JCPDS card number 71-2089) ] and quartz (JCPDS card number 85-0504) (see biological sandstone b in FIG. 3).
Fig. 2 shows an SEM image of desert sand. The desert shape is irregular spherical and the surface is rough. The particles are not uniform in size and range from 100 to 300 microns in diameter (figure 2). SEM images of biological sandstones cemented with biological magnesium ammonium phosphate cement (as shown in figure 3). The form of the biological cement is irregular sheet in the biological sandstone, and desert pores can be filled with biological magnesium ammonium phosphate cement.
The biological sandstone was subjected to a wind erosion test at an average wind speed of 12.0 m/s. The wind erosion rate of the desert is 798g/m 2/h. The biological magnesium ammonium phosphate cement is sprayed on the surface of the desert for 1 time and 3 times respectively, and the average hardness of the two biological sandstones is 4.4 and 12.3 respectively. The wind erosion rates of the two biological sandstones which are sprayed for 1 time and 3 times are respectively 0g/m2H and 0g/m2H is used as the reference value. These results show that the sand dust can be effectively suppressed by spraying the biological magnesium ammonium phosphate cement 1 and 3 times.
Three-dimensional pore structure evolution of biological sandstone the cementation of biological magnesium phosphate hexahydrate in sandstone was studied by X-ray computed tomography. The maximum defect volume of the biological sandstone sprayed for 3 times is 134mm3. The average porosity of the biological sandstone measured by XRCT is 18.39%. The results further demonstrate that the biological magnesium ammonium phosphate cement fills the porosity between the desert sand grains well.
Mixing MgCl2·2H2O and urea (3 mol/L) and K2HPO4·3H2S of O (2 mol/L)The porosarcina-Pasteurii mixed solution is sprayed on the surface layer of the desert sand for 3 times in a circulating way. When the sand solidification layer is destroyed, rye grass is planted in desert sand. Ryegrass can grow successfully in the blank after 4 days. However, ryegrass can also grow in the microbial cement group after 11 days. Since the microbial cement group sand environment is weakly alkaline, ryegrass is delayed in growth in the microbial cement group. After 15 days in the blank and microbial cement groups, the growth rate of ryegrass was consistent with that of the blank.
The loose desert sand can be effectively cemented by the biological magnesium ammonium phosphate cement. It is a new type of biological cement, consolidating loose sand particles into sandstone, and converting ammonia and ammonium salts into magnesium ammonium phosphate (MgNH)4PO4（H2O) 6). XRD results show that the cementing products in the biological sandstone are mainly MgNH4PO4（H2O) 6. MgNH in biological sandstone4PO4（H2O) 6 has an irregular sheet-like form. The average hardness of biological magnesium ammonium phosphate cement spray nos. 1 and 3 were 4.4 and 12.3, respectively. XRCT shows that the maximum defect volume and the average porosity of the biological sandstone are respectively 134mm3And 18.39%.
Claims (2)
1. A method for consolidating desert sand by biological magnesium ammonium phosphate cement is characterized by comprising the following steps: the method comprises the following steps:
A. firstly, preparing a nutrient solution, wherein the pH =7, and the nutrient solution is a mixed solution of 15g/L casein and 5g/L soyabean peptone; then selecting and culturing the spore bacillus Sporosarcina-Pasteurii; measuring the OD value of the sample in the range of 1.5-1.9 by an ultraviolet spectrophotometer;
B. MgCl is added into the culture solution of spore pasteur bacillus according to 3mol/L2·2H2Adding K into the mixed solution of O and urea according to the proportion of 3mol/L2HPO4·3H2O forms a biological curing solution, namely biological magnesium ammonium phosphate cement slurry; in the formulation K2HPO4·3H2O and MgCl2·2H2The molar ratio of O to urea is 1: 1: 1;
C. solidifying the organismThe spraying amount of the solution per unit area is 3L/m2The biological sandstone is formed after the desert sand is stored in the air for 3 days after being sprayed, the storage temperature is required to be 5-45 ℃, and the two end point values of the storage temperature fluctuate within the range of 0.5 ℃.
2. The method for testing the performance of the biological sandstone cured by the method for consolidating desert sand by using the biological magnesium ammonium phosphate cement as claimed in claim 1 is characterized by comprising the following steps of: the method comprises the following steps:
A. and (3) testing the rheumatism rate: firstly, spraying the biological curing solution on the surface of the desert for 1 time, taking out the biological sandstone, and weighing to obtain a quality value, and spraying the biological curing solution on the surface of the desert for 3 times, taking out the biological sandstone, and weighing to obtain a quality value; then wrapping the bottoms and the peripheries of the two biological sandstone samples which are generated by spraying by adopting aluminum sheets, only exposing the upper parts of the biological sandstone, and then placing the biological sandstone into an inner hole of an air duct, wherein the air duct comprises a barrel body, a fan is fixed at one end of the barrel body, a sample fixing groove is arranged in the barrel body, the sample fixing groove is formed by surrounding boards, and the two biological sandstone samples which are generated by spraying are uniformly placed into the fixing groove; then starting a fan to control the average wind speed to be 12.0m/s, and continuously blowing for 1 hour; finally, calculating to obtain the wind erosion rates of the two biological sandstones which are respectively sprayed for 1 time and 3 times and are respectively 0g/m by weighing the mass value of the biological sandstones before blowing and subtracting the mass value after blowing2H and 0g/m2/h；
B. Spraying the biological curing solution on the surface of the desert for 3 times and taking out a biological sandstone sample; the three-dimensional pore structure evolution of the biological sandstone sample researches the cementation of the biological magnesium ammonium phosphate in the sandstone by an X-ray computed tomography technology, and the maximum defect volume of the biological sandstone sprayed for 3 times is 134mm3The XRCT determined average porosity of the biological sandstone is 18.39%, and the result further proves that the biological magnesium ammonium phosphate cement can well fill the porosity among the desert sand grains;
C. circularly spraying the biological curing solution on the surface layer of the desert sand for 3 times and taking a biological sandstone sample; and (2) loading the biological sandstone sample into a container, planting ryegrass into the biological sandstone sample when a sand grain solidified layer is damaged, wherein the ryegrass can successfully grow in a blank group after 4 days, and can also grow in the biological sandstone sample after 11 days, the ryegrass can delay growth in the microbial cement group because the sand environment of the microbial cement group is weakly alkaline, and the growth rate of the ryegrass is consistent with that of the blank group after the ryegrass grows in the blank group and the biological sandstone sample group for 15 days.
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| JP4824644B2 (en) * | 2007-07-24 | 2011-11-30 | Ｊｃｋ株式会社 | Solid sand method for vegetation greening |
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