CN108298847A - The method of biological ammonium magnesium phosphate cement consolidation Desert Sand and the performance test methods for the biological sandstone being formed by curing - Google Patents
The method of biological ammonium magnesium phosphate cement consolidation Desert Sand and the performance test methods for the biological sandstone being formed by curing Download PDFInfo
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- CN108298847A CN108298847A CN201810213621.6A CN201810213621A CN108298847A CN 108298847 A CN108298847 A CN 108298847A CN 201810213621 A CN201810213621 A CN 201810213621A CN 108298847 A CN108298847 A CN 108298847A
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- sandstone
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- sand
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- MXZRMHIULZDAKC-UHFFFAOYSA-L ammonium magnesium phosphate Chemical compound [NH4+].[Mg+2].[O-]P([O-])([O-])=O MXZRMHIULZDAKC-UHFFFAOYSA-L 0.000 title claims abstract description 31
- 239000004568 cement Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000007596 consolidation process Methods 0.000 title claims description 7
- 238000011056 performance test Methods 0.000 title abstract description 8
- 239000000243 solution Substances 0.000 claims abstract description 39
- 239000004576 sand Substances 0.000 claims abstract description 20
- 239000007921 spray Substances 0.000 claims abstract description 16
- 235000015097 nutrients Nutrition 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 12
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004202 carbamide Substances 0.000 claims abstract description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 8
- 230000003628 erosive effect Effects 0.000 claims abstract description 8
- 241000193395 Sporosarcina pasteurii Species 0.000 claims abstract description 7
- 239000005018 casein Substances 0.000 claims abstract description 5
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000021240 caseins Nutrition 0.000 claims abstract description 5
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000209082 Lolium Species 0.000 claims description 23
- 244000005700 microbiome Species 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 9
- 238000002591 computed tomography Methods 0.000 claims description 5
- 239000003344 environmental pollutant Substances 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims description 4
- 231100000719 pollutant Toxicity 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 239000002344 surface layer Substances 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 239000011083 cement mortar Substances 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000010998 test method Methods 0.000 claims 1
- 239000000428 dust Substances 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 18
- 239000000203 mixture Substances 0.000 description 4
- 238000001878 scanning electron micrograph Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 description 1
- 239000004137 magnesium phosphate Substances 0.000 description 1
- 229960002261 magnesium phosphate Drugs 0.000 description 1
- 229910000157 magnesium phosphate Inorganic materials 0.000 description 1
- 235000010994 magnesium phosphates Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B12/00—Cements not provided for in groups C04B7/00 - C04B11/00
- C04B12/02—Phosphate cements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
- G01N15/088—Investigating volume, surface area, size or distribution of pores; Porosimetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N33/243—Earth materials for determining biological parameters concerning composting, biodegradability or bioavailability
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
- G01N33/248—Earth materials related to manure as a biological product
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Environmental & Geological Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geology (AREA)
- Remote Sensing (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Ceramic Engineering (AREA)
- Dispersion Chemistry (AREA)
- Materials Engineering (AREA)
- Structural Engineering (AREA)
- Organic Chemistry (AREA)
- Pit Excavations, Shoring, Fill Or Stabilisation Of Slopes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a kind of methods that biological ammonium magnesium phosphate cement consolidates Desert Sand, include the following steps：A, nutrient solution, pH=7 of nutrient solution are prepared first, and nutrient solution is the mixed solution of casein 15g/L and soy peptone 5g/L；Then spore Pasteur bacillus is selected（Sporosarcina‑Pasteurii）And it cultivates；B, MgCl22H2O and urea mixed solution is added by 3mol/L in spore Pasteur bacillus culture solution, K2HPO43H2O, which is added, by 3mol/L forms biological curing solution；C, by biological curing solution, unit area fountain height is 3L/m2, recycle and be sprayed on Desert Sand, form biological sandstone after Desert Sand preserves 3 days in air after sprinkling.The invention also discloses the performance test methods for the biological sandstone that the method that above-mentioned biological ammonium magnesium phosphate cement consolidates Desert Sand is formed by curing.The biological curing spray solution that method provided by the invention is prepared forms biological sandstone on Desert Sand, can substantially reduce wind erosion rate, the sand and dust for effectively inhibiting desert area to generate.
Description
Background technology
Desertification and sandstorm are that northwest China one endangers greatly, mainly using afforestation in traditional treatment method
Mode cure soil, stop sand and dust, but this method needs long-term build that can just tell on, if it is possible in conjunction with some
Short-term means reach and quickly inhibit sand and dust, and prolonged curing sandy soil, then more preferably.
Invention content
Technical problem to be solved by the invention is to provide a kind of methods that biological ammonium magnesium phosphate cement consolidates Desert Sand, should
Method being capable of more environmentally-friendly, more quick solidification sandy soil.The technical problems to be solved by the invention further include providing in one kind
The performance test methods for the biological sandstone that the method for stating biological ammonium magnesium phosphate cement consolidation Desert Sand is formed by curing.
For this purpose, the method for biology ammonium magnesium phosphate cement consolidation Desert Sand provided by the invention, it is characterized in that：Including following step
Suddenly：
A, nutrient solution, pH=7 of nutrient solution are prepared first, and nutrient solution is casein 15g/L's and soy peptone 5g/L
Mixed solution；Then spore Pasteur bacillus is selected（Sporosarcina-Pasteurii）And it cultivates；Ultraviolet spectrometry light
Degree meter measures its OD value within the scope of 1.5-1.9；
B, MgCl22H2O and urea mixed solution is added by 3mol/L in spore Pasteur bacillus culture solution, presses
3mol/L is added K2HPO43H2O and forms biological curing solution, i.e., biological ammonium magnesium phosphate cement mortar；K in formula2HPO4·
3H2O and MgCl22H2O, urea molar ratio be 1: 1: 1；
C, by the biological curing solution, unit area fountain height is 3L/m2, it recycles and is sprayed on Desert Sand, desert after sprinkling
Husky preservation in air forms biological sandstone after 3 days, and storage temperature requires 5-45 ± 0.5 DEG C.
The present invention also provides the biological sandstone that the method that above-mentioned biological ammonium magnesium phosphate cement consolidates Desert Sand is formed by curing
Performance test methods, including：
A, rheumatism rate is tested：It by the biological curing spray solution desert surface 1 time and takes out biological sandstone first and claims to obtain quality
Value by the biological curing spray solution desert surface 3 times and takes out biological sandstone and claims to obtain mass value；Then two kinds of sprinklings are given birth to
At biological sandstone sample bottom and surrounding wrapped up using aluminium flake and only expose the top of biological sandstone, then be placed in the interior of air duct
Kong Zhong, air duct include cylinder, and cylinder one end is fixed with wind turbine, sample fixing is provided in cylinder, sample fixing is by surrounding
Coaming plate surround to be formed, two kinds sprinkling generate biological sandstone samples be uniformly placed in the fixing groove；Then starting wind turbine will
Mean wind speed control is persistently dried 1 hour in 12.0m/s；It subtracts and blows finally by mass value before the blowing for weighing biological sandstone
It is respectively 0g/m2/h and 0g/m2/h that mass value, which is calculated two kinds of biological sandstone and sprays 1 time and 3 times wind erosion rate, after wind；
B, by the biological curing spray solution desert surface 3 times and biological sandstone sample is taken out；The three-dimensional of biological sandstone sample
It is cementing in sandstone that biological ammonium magnesium phosphate is studied in pore structure evolution by X ray computer tomography, measures
The greatest drawback volume for spraying 3 secondary pollutant sandstone is 134mm3, and the biological sandstone average pore that XRCT is measured is 18.39%, knot
Fruit further demonstrates biological ammonium magnesium phosphate cement can fill porosity between the sand grains of desert well；
C, biological curing solution cycle is sprayed at Desert Sand surface layer 3 times and takes biological sandstone sample；Biological sandstone is tried
Sample is fitted into container, and when grains of sand cured layer is destroyed, by rye grass planting to biological sandstone sample, rye grass is after 4 days
Can in blank group successful growth, rye grass can also grow in biological sandstone sample after 11 days, due to microorganism water
Mud group sand environment is weakly alkaline, so rye grass growth-delaying in microorganism cement group, in rye grass in blank group and life
After object sandstone sample group is grown 15 days, the rate of growth of rye grass is consistent with blank group.
Technique effect provided by the invention：1, using biological ammonium magnesium phosphate cement solidification Desert Sand, due to using life
Object curing, environmental pollution is few, does not interfere with the later stage and plants trees；It 2, can be in the sand using biological curing solution
Rapid curing forms biological sandstone, and so that sandy soil is raised is inhibited；3, the performance test methods of above-mentioned biological sandstone are simple and effective,
Each item data such as wind erosion rate can quickly be obtained.
Description of the drawings
Fig. 1 is Desert Sand（a）With biological sandstone（b）X-ray diffractogram；
Fig. 2 is the SEM image of Desert Sand；
Fig. 3 is the SEM image of biological sandstone.
Specific implementation mode
With reference to embodiments, the present invention will be described in further detail, described in specific embodiment only use
To explain the present invention, it is not used to limit invention.
The method of biology ammonium magnesium phosphate cement consolidation Desert Sand provided by the invention, includes the following steps：
A, nutrient solution, pH=7 of nutrient solution are prepared first, and nutrient solution is casein 15g/L's and soy peptone 5g/L
Mixed solution；Then spore Pasteur bacillus is selected（Sporosarcina-Pasteurii）And it cultivates；Ultraviolet spectrometry light
Degree meter measures its OD value within the scope of 1.5-1.9；
B, MgCl22H2O and urea mixed solution is added by 3mol/L in spore Pasteur bacillus culture solution, presses
3mol/L is added K2HPO43H2O and forms biological curing solution, i.e., biological ammonium magnesium phosphate cement mortar；K in formula2HPO4·
3H2O and MgCl22H2O, urea molar ratio be 1: 1: 1；
C, by the biological curing solution, unit area fountain height is 3L/m2, it recycles and is sprayed on Desert Sand, desert after sprinkling
Husky preservation in air forms biological sandstone after 3 days, and storage temperature requires 5-45 ± 0.5 DEG C.
The present invention also provides the biological sandstone that the method that above-mentioned biological ammonium magnesium phosphate cement consolidates Desert Sand is formed by curing
Performance test methods, including：
A, rheumatism rate is tested：It by the biological curing spray solution desert surface 1 time and takes out biological sandstone first and claims to obtain quality
Value by the biological curing spray solution desert surface 3 times and takes out biological sandstone and claims to obtain mass value；Then two kinds of sprinklings are given birth to
At biological sandstone sample bottom and surrounding wrapped up using aluminium flake and only expose the top of biological sandstone, then be placed in the interior of air duct
Kong Zhong, air duct include cylinder, and cylinder one end is fixed with wind turbine, sample fixing is provided in cylinder, sample fixing is by surrounding
Coaming plate surround to be formed, two kinds sprinkling generate biological sandstone samples be uniformly placed in the fixing groove；Then starting wind turbine will
Mean wind speed control is persistently dried 1 hour in 12.0m/s；It subtracts and blows finally by mass value before the blowing for weighing biological sandstone
It is respectively 0g/m2/h and 0g/m2/h that mass value, which is calculated two kinds of biological sandstone and sprays 1 time and 3 times wind erosion rate, after wind；
B, by the biological curing spray solution desert surface 3 times and biological sandstone sample is taken out；The three-dimensional of biological sandstone sample
It is cementing in sandstone that biological ammonium magnesium phosphate is studied in pore structure evolution by X ray computer tomography, measures
The greatest drawback volume for spraying 3 secondary pollutant sandstone is 134mm3, and the biological sandstone average pore that XRCT is measured is 18.39%, knot
Fruit further demonstrates biological ammonium magnesium phosphate cement can fill porosity between the sand grains of desert well；
C, biological curing solution cycle is sprayed at Desert Sand surface layer 3 times and takes biological sandstone sample；Biological sandstone is tried
Sample is fitted into container, and when grains of sand cured layer is destroyed, by rye grass planting to biological sandstone sample, rye grass is after 4 days
Can in blank group successful growth, rye grass can also grow in biological sandstone sample after 11 days, due to microorganism water
Mud group sand environment is weakly alkaline, so rye grass growth-delaying in microorganism cement group, in rye grass in blank group and life
After object sandstone sample group is grown 15 days, the rate of growth of rye grass is consistent with blank group.
Technical scheme of the present invention is explained in detail in the form of embodiment below：
Pass through specific nutrient solution（PH=7, the mixed solution of casein 15g/L and soy peptone 5g/L）Select spore
Sub- pasteurella（Sporosarcina-Pasteurii）And it cultivates.K2HPO4 is purchased from the limited public affairs of Chinese medicines group chemical reagent
Department.MgCl22H2O and urea are first prepared using preceding（3mol / L）Mixed solution.Desert Sand is obtained from Chinese Dunhuang
's.
By MgCl22H2O and urea（3mol / L）With containing K2HPO43H2O（2mol / L）'s
The cycle sprinkling of Sporosarcina-Pasteurii mixed solutions, finally, all biological sandstone all preserve 3 days in air
（30±2℃）.
Pass through X-ray diffraction（λ= 1.5406）Examine the chemical composition of Desert Sand and biological sandstone.Pass through scanning electron microscope
（SEM）With Genesis 60S energy dispersion energy dispersion X-ray spectrometers（EDS）Desert and biological sandstone are measured respectively
Form and element composition.The hardness of biological sandstone is tested by Shore durometer.According to the literature, the 3D rendering of biological sandstone is logical
Cross X ray computer tomography（XRCT）It measures.
Performance test
XRD confirms that the chemical composition in desert is quartz sand（JCPDS card numbers 85-0504）（With reference to the Desert Sand a in Fig. 3）.Biology
The ingredient of sandstone is ammonium magnesium phosphate-【MgNH4PO4（H2O）6（JCPDS card numbers 71-2089）】And quartz（JCPDS card numbers 85-
0504）Mixture（With reference to the biological sandstone b in Fig. 3）.
Fig. 2 shows the SEM image of Desert Sand.Desert form is in irregular spherical, rough surface.Granular size is uneven
It is even;Diameter range is from 100 to 300 micron（Fig. 2）.By the SEM image of the biological sandstone of biological ammonium magnesium phosphate cementing（Such as
Shown in Fig. 3）.The form of biological cement is irregular sheet in biological sandstone, and desert hole can be full of by biological ammonium phosphate
Magnesium cement.
In the case where mean wind speed is 12.0m/s, wind erosion test is carried out to biological sandstone.Desert rate of wind erosion is
798g / m2 / h.Biological ammonium magnesium phosphate cement sprays desert surface 1 time and 3 times respectively, the average hardness of two kinds of biological sandstone
Respectively 4.4 and 12.3.The wind erosion rate that two kinds of biological sandstone spray 1 time and 3 times be respectively 0g/m2/h and 0g/m2/
h.These results indicate that sand and dust can be effectively inhibited by being sprayed 1 time and 3 times with biological ammonium magnesium phosphate cement.
Biological magnesium phosphate six is studied in the three dimensional pore structures evolution of biological sandstone by X ray computer tomography
Hydrate is cementing in sandstone.The greatest drawback volume for spraying 3 secondary pollutant sandstone is 134mm3.The biological sandstone that XRCT is measured
Average pore is 18.39%.As a result further demonstrating biological ammonium magnesium phosphate cement can fill well between the sand grains of desert
Porosity.
By MgCl22H2O and urea（3mol / L）With containing K2HPO43H2O（2mol / L）'s
Sporosarcina-Pasteurii mixed solutions cycle is sprayed at Desert Sand surface layer 3 times.It, will when grains of sand cured layer is destroyed
In rye grass planting to sand field.Rye grass after 4 days can in blank group successful growth.However, rye grass can also
It is grown in microorganism cement group after 11 days.Since microorganism cement group sand environment is weakly alkaline, so rye grass is micro-
Growth-delaying in biological cement group.After blank group and microorganism cement group 15 days, the rate of growth of rye grass is consistent with blank group.
Loose Desert Sand can be effectively by biological ammonium magnesium phosphate cementing.This is a kind of novel biological cement,
The loose grains of sand are consolidated into sandstone, and ammonia and ammonium salt are changed into ammonium magnesium phosphate（MgNH4PO4（H2O）6）.XRD results are aobvious
Show that the agglutinate in biological sandstone is mainly MgNH4PO4（H2O）6.MgNH4PO4 in biological sandstone（H2O）6 form is not
Regular sheet.The average hardness that biological ammonium magnesium phosphate cement sprays No. 1 and No. 3 is respectively 4.4 and 12.3.XRCT display biologies
The greatest drawback volume and average pore of sandstone are respectively 134mm3With 18.39%.
Claims (2)
1. a kind of method of biology ammonium magnesium phosphate cement consolidation Desert Sand, it is characterized in that：Include the following steps：
A, nutrient solution, pH=7 of nutrient solution are prepared first, and nutrient solution is casein 15g/L's and soy peptone 5g/L
Mixed solution；Then spore Pasteur bacillus is selected（Sporosarcina-Pasteurii）And it cultivates；Ultraviolet spectrometry light
Degree meter measures its OD value within the scope of 1.5-1.9；
B, MgCl22H2O and urea mixed solution is added by 3mol/L in spore Pasteur bacillus culture solution, presses
3mol/L is added K2HPO43H2O and forms biological curing solution, i.e., biological ammonium magnesium phosphate cement mortar；K in formula2HPO4·
3H2O and MgCl22H2O, urea molar ratio be 1: 1: 1；
C, by the biological curing solution, unit area fountain height is 3L/m2, it recycles and is sprayed on Desert Sand, Desert Sand after sprinkling
Biological sandstone is formed after preserving 3 days in air, storage temperature requires 5-45 ± 0.5 DEG C.
2. a kind of property for the biological sandstone that the method for biological ammonium magnesium phosphate cement consolidation Desert Sand is formed by curing described in claim 1
Energy test method, it is characterized in that：Including：
A, rheumatism rate is tested：It by the biological curing spray solution desert surface 1 time and takes out biological sandstone first and claims to obtain quality
Value by the biological curing spray solution desert surface 3 times and takes out biological sandstone and claims to obtain mass value；Then two kinds of sprinklings are given birth to
At biological sandstone sample bottom and surrounding wrapped up using aluminium flake and only expose the top of biological sandstone, then be placed in the interior of air duct
Kong Zhong, air duct include cylinder, and cylinder one end is fixed with wind turbine, sample fixing is provided in cylinder, sample fixing is by surrounding
Coaming plate surround to be formed, two kinds sprinkling generate biological sandstone samples be uniformly placed in the fixing groove；Then starting wind turbine will
Mean wind speed control is persistently dried 1 hour in 12.0m/s；It subtracts and blows finally by mass value before the blowing for weighing biological sandstone
It is respectively 0g/m2/h and 0g/m2/h that mass value, which is calculated two kinds of biological sandstone and sprays 1 time and 3 times wind erosion rate, after wind；
B, by the biological curing spray solution desert surface 3 times and biological sandstone sample is taken out；The three-dimensional of biological sandstone sample
It is cementing in sandstone that biological ammonium magnesium phosphate is studied in pore structure evolution by X ray computer tomography, measures
The greatest drawback volume for spraying 3 secondary pollutant sandstone is 134mm3, and the biological sandstone average pore that XRCT is measured is 18.39%, knot
Fruit further demonstrates biological ammonium magnesium phosphate cement can fill porosity between the sand grains of desert well；
C, biological curing solution cycle is sprayed at Desert Sand surface layer 3 times and takes biological sandstone sample；Biological sandstone is tried
Sample is fitted into container, and when grains of sand cured layer is destroyed, by rye grass planting to biological sandstone sample, rye grass is after 4 days
Can in blank group successful growth, rye grass can also grow in biological sandstone sample after 11 days, due to microorganism water
Mud group sand environment is weakly alkaline, so rye grass growth-delaying in microorganism cement group, in rye grass in blank group and life
After object sandstone sample group is grown 15 days, the rate of growth of rye grass is consistent with blank group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810213621.6A CN108298847B (en) | 2018-03-15 | 2018-03-15 | Method for consolidating desert sand by biological magnesium ammonium phosphate cement and method for testing performance of biological sandstone formed by solidification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810213621.6A CN108298847B (en) | 2018-03-15 | 2018-03-15 | Method for consolidating desert sand by biological magnesium ammonium phosphate cement and method for testing performance of biological sandstone formed by solidification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108298847A true CN108298847A (en) | 2018-07-20 |
| CN108298847B CN108298847B (en) | 2021-02-02 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110512587A (en) * | 2019-08-28 | 2019-11-29 | 中建三局第二建设工程有限责任公司 | A kind of high temperature and high salt desert quickly fixating sand method based on sandstone covering |
| CN111377627A (en) * | 2020-02-18 | 2020-07-07 | 温州大学 | Method for reinforcing residual soil by novel microbial inorganic cementing material and spraying equipment thereof |
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| CN106947492A (en) * | 2017-02-24 | 2017-07-14 | 河海大学 | A kind of composite sand consolidation model and its preparation method and application |
| CN107189789A (en) * | 2017-06-28 | 2017-09-22 | 常州新之雅装饰材料有限公司 | A kind of ecological environment sand fixation material and preparation method thereof |
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| KR20070004289A (en) * | 2005-07-04 | 2007-01-09 | 현익근 | Motivate yellow sand breeze in the desert, collect yellow sand, compress it, solidify it, and install dikes in the desert to prevent desertification. |
| JP2009027933A (en) * | 2007-07-24 | 2009-02-12 | Jck Kk | Construction method for solidifying vegetation greening sand |
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
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| CN110512587A (en) * | 2019-08-28 | 2019-11-29 | 中建三局第二建设工程有限责任公司 | A kind of high temperature and high salt desert quickly fixating sand method based on sandstone covering |
| CN111377627A (en) * | 2020-02-18 | 2020-07-07 | 温州大学 | Method for reinforcing residual soil by novel microbial inorganic cementing material and spraying equipment thereof |
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| CN108298847B (en) | 2021-02-02 |
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