CN108294165A - 一种抗氧化饲料添加剂及其制备方法 - Google Patents

一种抗氧化饲料添加剂及其制备方法 Download PDF

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CN108294165A
CN108294165A CN201711412316.1A CN201711412316A CN108294165A CN 108294165 A CN108294165 A CN 108294165A CN 201711412316 A CN201711412316 A CN 201711412316A CN 108294165 A CN108294165 A CN 108294165A
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公衍军
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Qingdao Madil Biological Engineering Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements

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Abstract

本发明涉及一种抗氧化饲料添加剂,主要由以下质量份的组分配制而成,抗氧化肽复合物50‑60份,维生素C 5‑10份,维生素E 5‑10份,牛磺酸10‑15份,α‑硫酸锌5‑30份,本发明还公开了上述添加剂在牲畜饲料中的应用。本发明的抗氧化饲料添加剂可以广泛应用于牲畜饲料中,在牲畜饲粮中按照重量比添加比例为0.5%‑1%,本发明的抗氧化饲料添加剂可以明显提高机体的抗氧化能力,降低牲畜在养殖过程中的氧化应激和氧化损伤,本发明的抗氧化饲料添加剂中含有多种维生素有利于动物维持机体健康和高产。

Description

一种抗氧化饲料添加剂及其制备方法
技术领域
本发明涉及一种牲畜类饲料添加剂技术领域,具体地涉及一种抗氧化饲料添加剂及其制备方法。
背景技术
体内活性氧自由基具有一定的功能,如参与免疫和信号传导等过程。但过多的活性氧自由基就会过犹不及,导致动物正常细胞和组织被损坏,从而引起多种疾病。在正常情况下,动物体内的氧自由基一直维持在一个较低的水平,氧自由基处于消耗和产生的平衡状态,不会对机体产生不利影响。但是许多外源性或内源性刺激都可以打破这种平衡,造成氧自由基大量积累,从而对动物产生不利影响。氧化应激往往会带来牛羊等牲畜的肉质下降,严重者还会引发多种动物疾病等问题。
而常见的一些抗氧化剂例如没食子酸丙酯等吸收率不高而且稳定性差且都存在一定的毒副作用。而硫辛酸可以与维生素在体内形成一个抗氧化循环且容易被吸收。本发明的抗氧化剂主要成分含有微生物发酵鱼加工企业下脚料后制的抗氧化肽复合物,该复合物可以明显的清除羟自由基等。本发明所得抗氧化复合肽中含有活菌,包括枯草芽孢杆菌、纳豆芽孢杆菌、米曲霉及保加利亚乳杆菌等,这些细菌的孢子状态进入消化道后会在短期内大量繁殖形成种群优势,建立微生态平衡,促进有益厌氧微生物的繁殖,抑制有害细菌(大肠杆菌、沙门氏杆菌)的生长,从而预防腹泻、下痢等肠胃道疾病。
因此,与其它抗氧化剂相比本发明所得的抗氧化饲料添加剂具有更高的抗氧化活性。
发明内容
本发明涉及一种抗氧化饲料添加剂主要由以下组分质量份配制而成,抗氧化肽复合物50-60份,维生素C 5-10份,维生素E 5-10份,牛磺酸10-15份,α-硫酸锌5-30份。
步骤一,将鱼类加工下脚料晒干后,研磨成粉。
步骤二,用自来水配制成5%-10%的鱼类加工下脚料悬浮液。
步骤三,将悬浮液在121℃下,高温灭菌20min。
步骤四,按照5%-10%的接种量,接种复合菌剂于灭菌后的悬浮液中,在30-40℃下发酵培养48-72h。
步骤五,将发酵液冷冻干燥即为本发明所述抗氧化肽复合物。
进一步,所述鱼类加工下脚料为罗非鱼等加工下脚料。
进一步,所述复合菌剂主要是枯草芽孢杆菌、纳豆芽孢杆菌、米曲霉、保加利亚乳杆菌等组成,且比例为1:2:1:3。
步骤六,将所述抗氧化肽复合物50-60份,维生素C 5-10份,维生素E 5-10份,牛磺酸10-15份,α-硫酸锌5-30份,混合均匀。
步骤七,将所述抗氧化剂按照0.5%-1%的添加比例混入牲畜饲料中。
本发明与现有技术相比具有:本发明的一种抗氧化饲料添加剂可广泛应用于牲畜类饲料添加剂中,可以明显提高机体抗氧化能力,降低牲畜养殖过程中的氧化应激和氧化损伤,提高动物机体的免疫能力。
具体实施方式
下面结合具体实施例,进一步阐明本发明。
实施例1
本发明所述的抗氧化添加剂的制备主要由以下步骤组成:
步骤一,将鱼类加工下脚料晒干后,研磨成粉。
步骤二,用自来水配制成5%-10%的鱼类加工下脚料悬浮液。
步骤三,将悬浮液在121℃下,高温灭菌20min。
步骤四,按照5%-10%的接种量,接种复合菌剂于灭菌后的悬浮液中,在30-40℃下发酵培养48-72h。
步骤五,将发酵液冷冻干燥即为本发明所述抗氧化肽复合物。
所述鱼类加工下脚料为罗非鱼等加工下脚料。
所述复合菌剂主要是枯草芽孢杆菌、纳豆芽孢杆菌、米曲霉、保加利亚乳杆菌中的一种或几种组成,且比例为1:2:1:3。
步骤六,将所述抗氧化肽复合物50-60份,维生素C 5-10份,维生素E 5-10份,牛磺酸10-15份,α-硫酸锌5-30份,混合均匀。
步骤七,将所述抗氧化剂按照0.5%-1%的添加比例混入牲畜饲料中。
实施例2
本实施例作为一种优选的实施方式,
本实施例中,步骤六中,所述抗氧化肽复合物50份,维生素C 5份,维生素E 5份,牛磺酸10份,α-硫酸锌30份,混合均匀。
实施例3
本实施例中,步骤六中,将所述抗氧化肽复合物60份,维生素C 10份,维生素E 10份,牛磺酸15份,α-硫酸锌5份,混合均匀。
实施例4
本实施例中,步骤六中,将所述抗氧化肽复合物55份,维生素C 7份,维生素E 7份,牛磺酸13份,α-硫酸锌18份,混合均匀。
实验例5,对比实验:
将100头平均体重为50kg左右的小尾寒羊随机分成两个组,对照组饲喂基础饲粮,实验组在基础饲粮中添加1%的本发明的抗氧化饲料添加剂,饲养期为30天。
分别于正试期开始和结束时空腹12h后称重,统计各组的饲料消耗量,计算平均日采食量、平均日增重和料重比。试验结束从每个重复中各随机选取2只羊,采集前腔静脉血,测抗氧化指标(包括SOD、GSH-Px、T-AOC)。采用分光光度计以比色法测定。
表1抗氧化剂饲料对每只小尾寒羊的体重影响
项目 对照组 实验组
平均日采食量/g 500 450
平均日增重/g 129.79 139.43
料重比 3.85 3.22
由表1可以知道,实验组的平均日采食量小于对照组,但平均每日的增重实验组比对照组高7.42%,料重比实验组比对照组下降了19.56%。
表2抗氧化剂饲料对小尾寒羊血清抗氧化能力的影响
项目 对照组 实验组
超氧化物歧化酶/U·mL-1 113.92 125.64
谷胱甘肽过氧化物酶/U·mL-1 645.34 878.93
总抗氧化能力/U·mL-1 3.16 3.97
由表2可以知道,实验组的超氧化物歧化酶酶活力比对照组的酶活力提高了10.3%,而谷胱甘肽过氧化物酶的酶活力比对照组的酶活力提高了36.2%,而总抗氧化能力提高了59.2%。
由此说明本发明抗氧化饲料添加剂可以明显提高牲畜的抗氧化能力,减少氧化应激和氧化损伤。

Claims (3)

1.一种抗氧化饲料添加剂,其特征在于:主要由以下质量份的组分配制而成,
抗氧化肽复合物50-60份,维生素C 5-10份,维生素E 5-10份,牛磺酸 10-15份,α-硫酸锌 5-30份。
2.根据权利要求1所述的一种抗氧化饲料添加剂,其特征在于:抗氧化肽复合物为微生物发酵鱼加工企业下脚料接种复合菌剂制得。
3.一种抗氧化饲料添加剂的制备方法,其特征在于:其包括如下的步骤:
步骤一,将鱼类加工下脚料晒干后,研磨成粉;
步骤二,用自来水配制成5%-10%的鱼类加工下脚料悬浮液;
步骤三,将悬浮液在121℃下,高温灭菌20min;
步骤四,按照5%-10%的接种量,接种复合菌剂于灭菌后的悬浮液中,在30-40℃下发酵培养48-72h;所述复合菌剂为枯草芽孢杆菌、纳豆芽孢杆菌、米曲霉、保加利亚乳杆菌中的一种或几种组成的混合菌群;
步骤五,将发酵液冷冻干燥即为本发明所述抗氧化肽复合物;
进一步,所述鱼类加工下脚料为罗非鱼等加工下脚料;
进一步,所述复合菌剂主要是枯草芽孢杆菌、纳豆芽孢杆菌、米曲霉、保加利亚乳杆菌等组成,且比例为1:2:1:3;
步骤六,将所述抗氧化肽复合物50-60份,维生素C 5-10份,维生素E 5-10份,牛磺酸10-15份,α-硫酸锌 5-30份,混合均匀;
步骤七,将所述抗氧化剂按照0.5%-1%的添加比例混入牲畜饲料中。
CN201711412316.1A 2017-12-23 2017-12-23 一种抗氧化饲料添加剂及其制备方法 Pending CN108294165A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111938028A (zh) * 2020-07-27 2020-11-17 惠州市康维健生物科技有限公司 一种用于动物饲料的抗氧化剂配方

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1579198A (zh) * 2003-08-15 2005-02-16 中国农业大学 一种活性肽饲料添加剂及其制备方法和用途
CN104206744A (zh) * 2014-09-26 2014-12-17 江苏农林职业技术学院 一种抗氧化饲料添加剂及其应用
CN105901303A (zh) * 2016-05-06 2016-08-31 浙江海洋大学 一种优质鱼粉蛋白

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1579198A (zh) * 2003-08-15 2005-02-16 中国农业大学 一种活性肽饲料添加剂及其制备方法和用途
CN104206744A (zh) * 2014-09-26 2014-12-17 江苏农林职业技术学院 一种抗氧化饲料添加剂及其应用
CN105901303A (zh) * 2016-05-06 2016-08-31 浙江海洋大学 一种优质鱼粉蛋白

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111938028A (zh) * 2020-07-27 2020-11-17 惠州市康维健生物科技有限公司 一种用于动物饲料的抗氧化剂配方

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