CN108294009A - A kind of bactericidal composition containing biphenyl pyrrole bacterium amine and metconazole - Google Patents

Abstract

The present invention relates to a kind of bactericidal composition and application thereof containing biphenyl pyrrole bacterium amine and metconazole, it is especially a kind of using biphenyl pyrrole bacterium amine and metconazole as the bactericidal composition of main active and its application.The bactericidal composition is made of two kinds of active ingredients of biphenyl pyrrole bacterium amine and metconazole and auxiliary agent, excipients, wherein the weight percent of the two is 1~80:80~1.The bactericidal composition can generate higher synergistic function, overcome and delayed the generation of fungi drug resistance, expand prevention spectrum, sterilization speed is fast, reduces drug cost, alleviates toxicity.The present invention can be prevented by the fungus-caused multiple diseases such as Fungi Imperfecti, sac fungus, basidiomycetes on crops, and especially leaf spot control effect caused by the powdery mildew, rust and Septoria to various crops protrudes, with obvious effects to be better than single dose.

Description

A kind of bactericidal composition containing biphenyl pyrrole bacterium amine and metconazole

Technical field

The present invention relates to technical field of pesticide combination, more particularly to a kind of sterilization containing biphenyl pyrrole bacterium amine and metconazole Composition.

Background technology

With the promotion that the variation and pesticide of cropping system largely use, the fungicide kind of single-activity component is agricultural That there are bactericidal ranges on bactericidal agent for preventing and treating is narrow, control effect is poor, dispenser dosage is also easy to produce greatly poisoning and drug resistance, application times Many drawbacks such as more, exacerbation environmental pollution have clearly resulted in a large amount of underproduction of crops and being significantly increased for production cost.For Prevent the resistance germ that agriculturally generates, a kind of method be release it is new with new component of the existing cost without mutual resistance but new Active ingredient development cost it is high, the development cycle is long, and does not all catch up with the resistance speed of germ generation forever.Others are done Method such as crop structure adjustment, different pesticide rotations etc., during practical operation, it is difficult to really play apparent effect.

Biphenyl pyrrole bacterium amine (Bixafen) belongs to line grain for the pyrazole amide series bactericidal agent of Bayer Cropscience Co., Ltd's exploitation Succinate dehydrogenase inhibitors in body respiratory chain, by interfering on the Complex II in pathogen mitochondrial respiratory electron transport chain Succinate dehydrogenase, inhibit mitochondrial function, prevent its generate energy, inhibit growth of pathogenic bacteria, eventually lead to its death.Connection Benzene pyrrole bacterium amine be systemic fungicide, have extensive fungicidal spectrum, can effectively prevent on cereal crops by sac fungus, basidiomycetes and Important disease caused by Fungi Imperfecti.Experiment proves that biphenyl pyrrole bacterium amine presents excellent preventive effect to many diseases on wheat crops, Such as wheat leaf blight, leaf rust, stripe rust, eye spot and maculopathy and net blotch of barley, pseudomonas sp, moire Disease and leaf rust etc., and can prevent and leaf caused by the Septoria pathogen of resistance is generated to methoxy acrylic bactericide Pinta etc..According to another report, biphenyl pyrrole bacterium amine has treatment and protecting effect well to apple mildew.In addition, the medicament is to planting Object physiology plays the role of positive, can enhance crop anti-adversity, improve crop yield.

Metconazole (Metconazole) is the triazole bactericidal agent of Japanese Wu Yu companies exploitation, from structure and traditional Triazole type has larger difference, the mechanism of action of metconazole to be no different with other azoles fungicides, is C- in ergosterol biosynthesis 14 demethylation enzyme inhibitors, but then difference is larger for activity profile, and two kinds of isomers have bactericidal activity, but Cis activity is higher than anti- Formula.The antifungal spectrum of metconazole is very extensive, and activity is splendid.It applies to cereal crops septoria musiva, fusarium in metconazole field Planting disease with handle rest fungus has excellent effect.For metconazole compared with traditional biocide agent, dosage is extremely low and prevents cereal and plants sick range very Extensively, antagonism head blight, powdery mildew, rust, glume blight have excellent preventive effect.

Different cultivars ingredient is compounded, and synergy with that can improve practical control effect, reduces the use of pesticide well Amount, additionally aids the generation for delaying germ drug resistance, is the important means of integrated control.We screen indoors and field trial On the basis of, the compounded combination of biphenyl pyrrole bacterium amine and metconazole is filtered out, there is apparent synergistic effect.

Invention content

The present invention is intended to provide a kind of complex composition, and it is made into that resistance risk is small, at low cost, drug effect is long, environment Pollute small agricultural double-crossed bactericidal composition.

The technical scheme is that：Agricultural double-crossed bactericidal composition, active ingredient are biphenyl pyrrole bacterium amine and leaf bacterium Azoles compounds.

The weight ratio of the active ingredient biphenyl pyrrole bacterium amine and metconazole is 1:80~80:1, preferably 1:10~10： 1.

Above-mentioned active ingredient biphenyl pyrrole bacterium amine can be bought with metconazole by various commercial channel.

Any one dosage form of suitable agricultural use can be prepared by known methods into the bactericidal composition of the present invention, compared with Good dosage form is missible oil, aqueous emulsion, microemulsion, aqueous suspension agent, oil-suspending agent, suspended emulsion, wettable powder or water-dispersible grain Agent.

Further include Agrotechnical formulation auxiliary in addition to active ingredient biphenyl pyrrole bacterium amine and metconazole in above-mentioned bactericidal composition Ingredient.Active ingredient biphenyl pyrrole bacterium amine is 1% with metconazole total weight percentage shared in the bactericidal composition ~90%, preferably 10%~80%.

The pesticidal preparations auxiliary element includes carrier and auxiliary agent.

The carrier be water, solvent or filler in one kind, two kinds or three kinds, the preferred deionized water of water.

The solvent is selected from n,N-Dimethylformamide, cyclohexanone, toluene, dimethylbenzene, dimethyl sulfoxide (DMSO), methanol, second Alcohol, trimethylcyclohexanone, n-octylpyrrolidone, n,N-Dimethylformamide, ethanol amine, triethanolamine, isopropylamine.N- Methyl pyrrolidone, propyl alcohol, butanol, ethylene glycol, diethylene glycol, ethylene glycol monomethyl ether, butyl ether, ethanol amine, isopropylamine, ethyl acetate Or the mixture of one or more compositions in acetonitrile.

The filler is in kaolin, diatomite, bentonite, attapulgite, white carbon, starch or precipitated calcium carbonate The mixture of one or more compositions.

The auxiliary agent includes at least a kind of surfactant and can be also added antifreeze according to different use occasions and demand Other functional aids such as agent, thickener, stabilizer, disintegrant, antifoaming agent.

The one kind or four kinds of the surfactant in emulsifier, dispersant, wetting agent or bleeding agent.Above-mentioned surface Activating agent is the single dose or complex preparation of common nonionic surfactant or anion surfactant.

Other described functional aids one kind or five in antifreezing agent, thickener, stabilizer, disintegrant or antifoaming agent Kind.

The emulsifier is selected from agriculture breast 500# (alkyl benzene calcium sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphorus Acid esters), 600# phosphates (phenyl phenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether Disulfonic acid magnesium salts, triethanolamine salt, agriculture breast 400# (benzyl dimethyl phenol polyoxyethyl ether), Nongru-700 # (alkylphenol formaldehyde trees Fat polyoxyethyl ether), peaceful breast 36# (phenethyl phenol formaldehyde resin polyoxyethyl ether), agriculture breast 1600# (phenethyl phenol polyoxyethanyls Polypropylene-base ether), ethylene oxide-propylene oxide block copolymer, OP serial (nonylphenol polyoxyethylene ether), the serial (castor-oil plants of BY Oily polyoxyethylene ether), agriculture breast 33# (alkylaryl polyoxyethylene poly-oxygen propylene aether), Span series (sorbitol anhydride monostearate), It is a kind of or more in TWEEN Series (anhydrous sorbitol resin acid ester APEO) or AEO series (fatty alcohol polyoxyethylene ether) The mixture of kind composition.

The dispersant be selected from polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulfat, Alkyl benzene calcium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkyl phenol polyoxyethylene ether, aliphatic amine polyoxyethylene ether, fatty acid polyglycol The mixture of one or more compositions in ethylene oxide ether or glycerol fatty acid ester polyoxyethylene ethe.

The wetting agent is selected from lauryl sodium sulfate, neopelex, Nekal BX, wetting and penetrating agent F, soap The mixture of one or more compositions in angle powder, silkworm excrement or spaonin powder.

The bleeding agent is selected from penetrating agent JFC (fatty alcohol polyoxyethylene ether), penetrant t (diisooctyl maleate Sulfonate), the mixture of azone or one or more compositions in organosilicon.

The mixture of one or more compositions of the antifreezing agent in ethylene glycol, propylene glycol, glycerine or urea.

One kind in xanthans, polyvinyl alcohol, bentonite, carboxymethyl cellulose or aluminium-magnesium silicate of the thickener or The mixture of a variety of compositions.

The stabilizer is selected from one kind or more of epoxidized soybean oil, epoxychloropropane, BHT, ethyl acetate, triphenyl phosphate The mixture of kind composition.

The disintegrant is selected from bentonite, urea, ammonium sulfate, aluminium chloride, low-substituted hydroxypropyl cellulose, lactose, lemon The mixture of acid, succinic acid or one or more compositions in sodium bicarbonate.

The antifoaming agent is selected from silicone oil, silicone compound, C10~C20 saturated fat acids compound or C8~C10 The mixture of one or more compositions in aliphatic alcohols compound.

Above-mentioned substance can be commercially available.

The present invention bactericidal composition can made of dosage form be missible oil, aqueous emulsion, microemulsion, oil-suspending agent, suspension emulsion, One kind in wettable powder, aqueous suspension agent or water dispersible granules.

Compound biocide composite provided by the invention is drawn for preventing crop by fungies such as Fungi Imperfecti, sac fungus, basidiomycetes The multiple diseases risen especially protrude the powdery mildew of various crops, rust, leaf spot control effect, with obvious effects better than single Agent, and the administration dosage of each active ingredient can be reduced, reduce cost.

The beneficial effects of the present invention are：

1, expand fungicidal spectrum, not only to special efficacys such as crop powdery mildew, rust, but also to leaf spot, head blight, leaf blight etc. A variety of fungus-caused disease excellent effects；

2, synergistic effect is apparent, is not the simple superposition of each component activity, and drug effect greatly improves；

3, prevention drug effect is high, and the lasting period is long, and resistance risk is small；

4, the field rate of active ingredient declines, and reduces production and application cost, reduces pesticide residue and environmental pollution；

5, it is made of the active ingredient of different role mechanism, action site increases, and is conducive to overcome and delay the anti-medicine of germ The generation of property.

Specific implementation mode

The present invention is illustrated with reference to embodiments, but does not limit the invention to these specific implementation modes.It is real It applies percentage in example to be weight percentage, the treatment dosage of field efficacy is effective ingredient consumption.

One, embodiment is prepared

1, wettable powder

Preparation method：In Example formulations ratio, active ingredient biphenyl pyrrole bacterium amine and metconazole are added in carrier, and Surfactant and other functional aids are wherein added, mixing remixes obtained wettable powder after air-flow crushing.Mainly Equipment has mixing machine, airslide disintegrating mill.

Embodiment 1：(60% biphenyl pyrrole bacterium amine metconazole wettable powder) 2:1

Formula：Biphenyl pyrrole bacterium amine, 40%；Metconazole, 20%；Wetting agent lauryl sodium sulfate, 2%；Dispersant is wooden Plain sulfoacid calcium, 8%；Filler white carbon, 18%, filler kaolin, surplus.

Embodiment 2：(80% biphenyl pyrrole bacterium amine metconazole wettable powder) 3:1

Formula：Biphenyl pyrrole bacterium amine, 60%；Metconazole, 20%；Wetting agent lauryl sodium sulfate, 2%；Dispersant is wooden Plain sulfoacid calcium, 3%；Filler white carbon, 10%, filler kaolin, surplus.

2, water dispersible granules

Preparation method：In Example formulations ratio, active ingredient biphenyl pyrrole bacterium amine and metconazole are added in carrier, and Surfactant and other functional aids are wherein added, mixing adds 10~25% water, then through pinching after air-flow crushing It closes, be granulated, is dry, water dispersible granules product is made in screening；Or the powder that crushed is sprayed water in boiling granulator, is granulated, It is dry, it sieves product is made later.Capital equipment has mixing machine, airslide disintegrating mill, kneader, Squeezinggranulator, drying chamber or stream Change bed drying or boiling granulator, screening machine.

Embodiment 3：(50% biphenyl pyrrole bacterium amine metconazole water dispersible granules) 1:1

Formula：Biphenyl pyrrole bacterium amine, 25%；Metconazole, 25%；Dispersant Negel, 5%；Dispersant lignin Sodium sulfonate, 7%；Wetting agent lauryl sodium sulfate, 2%；Disintegrant ammonium sulfate, 5%；Precipitated calcium carbonate is mended, surplus.

Embodiment 4：(70% biphenyl pyrrole bacterium amine metconazole water dispersible granules) 2:5

Formula：Biphenyl pyrrole bacterium amine, 20%；Metconazole, 50%；Dispersant Negel, 5%；Dispersant lignin Sodium sulfonate, 7%；Wetting agent lauryl sodium sulfate, 2%；Disintegrant ammonium sulfate, 5%；Precipitated calcium carbonate is mended, surplus.

3, missible oil

Preparation method：Active ingredient biphenyl pyrrole bacterium amine and metconazole are added in carrier in Example formulations ratio, and Surfactant and other functional aids are wherein added, are uniformly mixed in being stirred kettle.It is stirred kettle Enamel reaction still, stainless steel stirred tank etc. can be selected.

Embodiment 5：(15% biphenyl pyrrole bacterium amine metconazole missible oil) 1:2

Formula：Metconazole, 5%；Biphenyl pyrrole bacterium amine, 10%；Emulsifier selects agriculture breast 500# and agriculture breast OP-10#, dosage point It Wei 4% and 5%；Solvent selection n,N-Dimethylformamide, 15%；Cyclohexanone, 10%；Dimethylbenzene, surplus.

4, aqueous emulsion

Preparation method：In Example formulations ratio by active ingredient biphenyl pyrrole bacterium amine and metconazole and solvent, emulsifier, help Solvent is added together, and makes to be dissolved into uniform oil phase；By part water, antifreeze, the others insecticides adjuvant such as antimicrobial is mixed It is combined into uniform water phase；The oil phase is added to the aqueous phase while being stirred in the reactor at high speed, and slowly plus water is until reach Phase inversion point opens cutter and carries out high speed shear, and remaining water is added, and shearing about half an hour, forms the water and milk of oil-in-water type Agent.

Embodiment 6：(18% metconazole biphenyl pyrrole bacterium aqueous amine emulsion) 1:2

Formula：Metconazole, 6%；Biphenyl pyrrole bacterium amine, 12%；Emulsifier selects agriculture breast 500# and agriculture breast 1601, dosage difference For 2% and 4%；Solvent selection n,N-Dimethylformamide, 15%；Dimethylbenzene, 20%；Antifreezing agent selects ethylene glycol, dosage 3%；Water, surplus.

5, microemulsion

Preparation method：In Example formulations ratio, by active ingredient biphenyl pyrrole bacterium amine and metconazole solvent and cosolvent It is completely dissolved, then adds other auxiliary agents, be uniformly mixed, water be eventually adding, after being sufficiently stirred, you can present invention sterilization is made Composite microemulsion preparation.

Embodiment 7：(16% biphenyl pyrrole bacterium amine metconazole microemulsion) 3:1

Formula：Biphenyl pyrrole bacterium amine, 12%；Metconazole, 4%；Solvent selects n,N-Dimethylformamide and cyclohexanone, uses Amount is respectively 15% and 20%；Emulsifier selects agriculture breast 602# and agriculture breast 500#, and dosage is respectively 9% and 6%；Water, surplus.

6, aqueous suspension agent

Preparation method：By formula rate, by active ingredient biphenyl pyrrole bacterium amine and metconazole, surfactant and other functions Property auxiliary agent be sequentially placed into reaction kettle, be mixed with water, through high speed shear, wet method is sanded, last homogeneous to filter up to product. Capital equipment is batching kettle, colloid mill or homogeneous mixing machine, sand mill.

Embodiment 8：(30% biphenyl pyrrole bacterium amine metconazole aqueous suspension agent) 2:1

Formula：Biphenyl pyrrole bacterium amine, 20%；Metconazole, 10%；Wetting agent NP-10,1%；Water dispersant polycarboxylate, 4%；It is respectively 1.2% and 0.12% that thickener, which selects aluminium-magnesium silicate and xanthans, dosage,；Antifreezing agent selection propylene glycol, 5%； Organically-modified silica alkane antifoaming agent, 0.2%；Water, surplus.

Embodiment 9：(40% biphenyl pyrrole bacterium amine metconazole aqueous suspension agent) 1:1

Formula：Biphenyl pyrrole bacterium amine, 20%；Metconazole, 20%；Wetting agent NP-10,1%；Water dispersant polycarboxylate, 5%；It is respectively 1.1% and 0.11% that thickener, which selects aluminium-magnesium silicate and xanthans, dosage,；Antifreezing agent selection propylene glycol, 5%； Organically-modified silica alkane antifoaming agent, 0.2%；Water, surplus.

7, oil-suspending agent

Preparation method：By formula rate, by active ingredient biphenyl pyrrole bacterium amine and metconazole, surfactant and other functions Property auxiliary agent be sequentially placed into reaction kettle, refueling is uniformly mixed, and through high speed shear, wet method is sanded, last homogeneous to filter up to product. Capital equipment is batching kettle, colloid mill or homogeneous mixing machine, sand mill.

Embodiment 10：(40% biphenyl pyrrole bacterium amine metconazole oil-suspending agent) 1:1

Formula：Biphenyl pyrrole bacterium amine, 20%；Metconazole, 20%；Emulsifier agriculture breast BY-125,7%；Oil dispersant polycarboxylic acids Salt, 8%；Thickener selects organobentonite, dosage 1.6%；Oil, surplus.

8, suspension emulsion

Preparation method：Solvent, surfactant is added in active ingredient biphenyl pyrrole bacterium amine in Example formulations ratio, adds water It is uniformly mixed, aqueous emulsion is made through high speed shear；Metconazole is added in carrier, and surfactant and other work(are added wherein Energy property auxiliary agent, is mixed with water, and through high speed shear, wet method is sanded, and suspending agent is made, finally by obtained suspending agent and water Emulsion high speed shear, it is homogeneous to filter up to product.Capital equipment is batching kettle, colloid mill or homogeneous mixing machine, sand mill.

Embodiment 11：(30% biphenyl pyrrole bacterium amine metconazole suspension emulsion) 2:1

Formula：Biphenyl pyrrole bacterium amine, 20%；Metconazole, 10%；Solvent N-methyl pyrilidone, dosage 25%；Emulsifier Agriculture breast 500# and agriculture breast OP-10# is selected, dosage is respectively 4% and 5%；Oil dispersant polycarboxylate, 8%；Thickener is selected Xanthans and aluminium-magnesium silicate, dosage are respectively 0.2% and 2.0%；Antifreeze glycol, dosage 3%；Water, surplus.

Two, BIOLOGICAL ACTIVITY EXAMPLES

BIOLOGICAL ACTIVITY EXAMPLES is divided into indoor biological activity Example and crop field BIOLOGICAL ACTIVITY EXAMPLES.

Bioassay example 1：Toxicity Determination of the composition to cucurbits powdery mildew bacterium

Subjects：Cucumber powdery mildew's pathogen (picks up from field)

Test method：Reference《People's Republic of China (PRC) agricultural industry criteria NY/T1156.11-2008》.Choose growth potential Consistent potting two panels leaf period cucumber seedling, each processing select 5 basins, number spare.It is pressed in 50PSI with Potter spray towers It sprays under power, per basin 5mL.5 concentration gradients are arranged referring to table 1, table 2, table 3, each medicament in test process.It is connect afterwards for 24 hours Kind a concentration of 3 × 10⁵The cucumber powdery mildew's pathogen conidial suspension of a spore/ml.Cucumber seedling to spray equivalent clear water is Blank control.Cucumber seedling is put into greenhouse after inoculation and is cultivated.After 10d, investigated according to the morbidity grade scale of powdery mildew of cucumber Disease index, and calculate control effect.

Powdery mildew of cucumber severity Scaling standard：

0 grade：Blade disease-free spot；

1 grade：Lesion area accounts for 5% or less the area of entire blade；

3 grades：Lesion area accounts for the 6%~10% of the area of entire blade；

5 grades：Lesion area accounts for the 11%~25% of entire blade area；×

7 grades：Lesion area accounts for the 25%~50% of entire blade area；

9 grades：Lesion area accounts for 50% or more of entire blade area.

Drug effect computational methods：

According to investigation result, disease index and preventive effect are calculated according to following (1), (2) formula.

In formula：CK₀、CK₁The respectively forward and backward disease index of blank district dispenser；PT₀、PT₁Respectively chemicals treatment

The forward and backward disease index of area's medicine.

Mixture toxicity test and interpretation of result：

The toxicity test of mixture is carried out according to mixed ratio with single agent virulence determination method.

Using the logarithm of drug concentration (mg/L) as independent variable x, using the niqueMin of corrected mortality as dependent variable y, respectively Virulence regression equation is established, the EC of single dose and each proportioning mixture is calculated using DPS softwares₅₀Poison altogether is calculated according to the abundant methods of Sun Yun Coefficient (CTC).Co-toxicity coefficient CTC, calculation formula are as follows：(using metconazole as standard agent, toxicity index 100)：

The EC of toxicity index (TI)=metconazole of biphenyl pyrrole bacterium amine₅₀The EC of/biphenyl pyrrole bacterium amine₅₀×100

The EC of true toxicity index (ATI)=metconazole of M₅₀The EC of/M₅₀×100

TI × P biphenyl pyrrole bacterium amine of TI × P metconazoles of theoretical toxicity index (TTI)=metconazole of M+biphenyl pyrrole bacterium amine

TTI × 100 of the ATI/M of co-toxicity coefficient (CTC)=M of M

In formula：

M is the mixture of biphenyl pyrrole bacterium amine and metconazole different ratio

P metconazoles are metconazole ratio shared in mixture

P biphenyl pyrrole bacterium amine is biphenyl pyrrole bacterium amine ratio shared in mixture.

Criterion：Co-toxicity coefficient ＜ 80 is antagonism, is summation action between 80-120, and ＞ 120 makees for synergy With.

1 biphenyl pyrrole bacterium amine of table compounds the The Toxicity Determination result to powdery mildew of cucumber with metconazole

As it can be seen from table 1 the suppression of the metconazole and biphenyl pyrrole bacterium amine composition of different proportion to powdery mildew of cucumber opportunistic pathogen Bacterium effect is different, the experimental results showed that it is than being listed in 20:1 to 1:There is synergistic effect in 20, wherein 5:1 to 1:In 5 ranges Synergy is notable.

Bioassay example 2：Biphenyl pyrrole bacterium amine compounds the field control effectiveness test to leaf spot of peanut with metconazole

According to " 17980.85-2004 pesticide field efficacy medicine test criterions of GB/T (two) --- the sterilization of medicine inspecting institute of the Ministry of Agriculture Agent prevents leaf spot of peanut ", test medicine, comparison medicament and the processing of the cell of blank control are arranged using random district's groups, cell Area 30m², 4 repetitions, the total dispenser of this experiment is twice.Leaf spot of peanut early stage is in when first time dispenser, after 7 days Apply second of medicine.Experiment is investigated 3 times altogether, including radix is investigated before medicine, i.e. investigation of premedication disease index, 7 days preventive effects after the 1st medicine It investigates, preventive effect investigation in 14 days after 2 medicines.Per cell 5 sampling surveys of diagonal line, fixed point investigates 4 plants, and every plant of investigation stem is complete Portion's blade, record investigation total number of sheets, the sick number of sheets at different levels.

Grade scale：

0 grade：Disease-free spot；

1 grade：Lesion area accounts for 5% or less entire leaf area；

3 grades：Lesion area accounts for the 6%-25% of entire leaf area；

5 grades：Lesion area accounts for the 26%-50% of entire leaf area；

7 grades：Lesion area accounts for the 50%-75% of entire leaf area；

9 grades：Lesion area accounts for 76% or more of entire leaf area.

Drug effect computational methods：

According to investigation result, disease index and preventive effect are calculated according to following (1), (2) formula.

In formula：CK₀、CK₁The respectively forward and backward disease index of blank district dispenser；PT₀、PT₁Respectively chemicals treatment The forward and backward disease index of area's medicine.

It the results are shown in Table 2.

The field control effectiveness test result of 2 composition for preventing and controlling leaf spot of peanut of table

As can be seen from Table 2,40% biphenyl pyrrole bacterium amine metconazole SC is with 1:1 ratio compounding prevention leaf spot of peanut Significant effect is higher than single dose, and effect is incremented by with the increase of dosage.Its quick-acting and lasting period are all more excellent, according to field mesh It surveys, within the range of experimental dose, plant growth is normal, and each treatment agent does not occur the poisoning phenomenon to peanut, pacifies to peanut Entirely.

Bioassay example 3：Biphenyl pyrrole bacterium amine is compounded with metconazole to wheat powdery mildew field control effectiveness test

Test medicine, comparison medicament and the processing of the cell of blank control are arranged using random district's groups, plot area 30m², 4 Secondary repetition, this experiment amount to dispenser twice.It is in wheat powdery mildew early stage when first time dispenser, is applied after 7 days second Medicine.It tests after medicine and investigates 3 times altogether, including radix is investigated before medicine, is i.e. investigation of premedication disease index, 7 days preventive effect tune after the 1st medicine It looks into, preventive effect investigation in 10 days after 2 medicines.Foundation《Pesticide field efficacy medicine test criterion》GB/17980.1-17980.53-2000, often Cell diagonal line fixes 5 sampling surveys, every investigation 0.25m²Plant, every plant is investigated whole blade state of an illness from top to bottom.

Severity Scaling standard is：

0 grade：Disease-free spot；

1 grade：Lesion area accounts for 5% or less entire leaf area；

3 grades：Lesion area accounts for the 6-15% of entire leaf area；

5 grades：Lesion area accounts for the 16-25% of entire leaf area；

7 grades：Lesion area accounts for the 26-50% of entire leaf area；

9 grades：Lesion area accounts for 50% or more of entire leaf area.

Drug effect computational methods：

According to investigation result, disease index and preventive effect are calculated according to following (1), (2) formula.

In formula：CK₀、CK₁The respectively forward and backward disease index of blank district dispenser；PT₀、PT₁Respectively chemicals treatment The forward and backward disease index of area's medicine.

Table 3 prevents wheat powdery mildew field control effectiveness test result

As can be seen from Table 3,40% biphenyl pyrrole bacterium amine metconazole SC is with 1:1 ratio compounding prevention wheat powdery mildew The significant effect of significant effect, complex preparation is higher than single dose, and quick-acting and lasting period are all more excellent, is ideal prevention The medicament of powdery mildew, and to wheat safety, and can effectively delay the generation of germ drug resistance.

Above-mentioned indoor biometrics and field control effectiveness test result show the sterilization of the invention containing biphenyl pyrrole bacterium amine and metconazole Composition has good control effect to powdery mildew of cucumber, wheat powdery mildew and leaf spot of peanut, and it is equal to trial crops Without apparent harmful effect, leaf color, growing way etc. are all normal, Target crops safety.Complex preparation not only increases anti-compared with single dose Effect, and it is made of the active ingredient of different role mechanism, and action site increases, and is conducive to overcome and delay germ drug resistance Generation, lower drug cost, reduce environmental pollution and pesticide residue, so this compounded combination has very in production practice Important meaning.

Claims (7)

1. a kind of bactericidal composition containing biphenyl pyrrole bacterium amine and metconazole, which is characterized in that its active ingredient is biphenyl pyrrole bacterium Amine is primary activity ingredient with metconazole, is subject to auxiliary agent and excipient compounds.

2. bactericidal composition according to claim 1, which is characterized in that the active ingredient biphenyl pyrrole bacterium amine and metconazole Weight ratio be 1:80~80:1.

3. bactericidal composition according to claim 2, which is characterized in that the active ingredient biphenyl pyrrole bacterium amine and metconazole Preferred weight ratio be 1:10~10:1.

4. bactericidal composition described in any one of claim 1 to 3, which is characterized in that the active ingredient biphenyl pyrrole Bacterium amine is 1%~90% with metconazole total weight percentage shared in the bactericidal composition, remaining is pesticide system Agent auxiliary element.

5. bactericidal composition according to claim 4, which is characterized in that the active ingredient biphenyl pyrrole bacterium amine and metconazole Shared total preferred weight percentage composition is 10%~80% in the bactericidal composition.

6. bactericidal composition according to claim 4, which is characterized in that the pesticidal preparations auxiliary element include carrier and Auxiliary agent.

7. bactericidal composition according to claim 4, which is characterized in that its dosage form is missible oil, aqueous emulsion, microemulsion, oil One kind in suspending agent, aqueous suspension agent, suspended emulsion, wettable powder or water dispersible granules.