CN108251490A - A kind of fermentation medium and fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium - Google Patents
A kind of fermentation medium and fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium Download PDFInfo
- Publication number
- CN108251490A CN108251490A CN201810332406.8A CN201810332406A CN108251490A CN 108251490 A CN108251490 A CN 108251490A CN 201810332406 A CN201810332406 A CN 201810332406A CN 108251490 A CN108251490 A CN 108251490A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- ganoderma lucidum
- lucidum mycelium
- medium
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of fermentation mediums and fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium.With weight, the composition of the fermentation medium is：200~300 μm of corn flour 2%~5%, analysis for soybean powder 1%~2%, glucose 1%~3%, potassium dihydrogen phosphate 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, 0~100ppm of pangamic acid, methyl jasmonate ol/L, surplus is water.Ganoderma lucidum mycelium ferments in above-mentioned fermentation medium, can extend fermentation time, obtains the fermentation glossy ganoderma mycelium product of high-content ganoderic acid A.
Description
Technical field
The invention belongs to edible and medical fungi technical field of producing, and in particular to a kind of raising liquid fermentation ganoderma lucidum mycelium
The fermentation medium and fermentation process of middle ganoderic acid A improves the ganoderic acid A contents of ganoderma lucidum mycelium.
Background technology
Ganoderma lucidum is described as " seocho " strengthened the body resistance to consolidate the constitution by China successive dynasties medicine scholar, has to the pharmacological action of ganoderma lucidum and largely grinds
Study carefully, research shows that ganoderma lucidum has extensive pharmacological action, as antitumor action, immunoregulation effect, anti-radiation and anti-chemotherapy are made
With, tranquilizing effect, cardiac stimulant and function of resisting myocardial ischemia, regulating blood lipid action, hypoglycemic effect, antiasthmatic effect, liver protection make
With, anti anoxia and anti-aging effects etc..As modern science gos deep into ganoderma lucidum research, until so far, being separated to 350 in total
Remaining kind of chemical composition, mainly polysaccharide, terpene, alkaloids, peptides, amino acids, sterols, vitamins etc..
Modern pharmacological research proves that ganodenic acid has antitumor, protection liver, anti-HIV-1 and HIV-1 protease activities
Property, antihistaminicum release, anti-aging, antimicrobial, reducing blood lipid, it is hypoglycemic the effects that.Ganodenic acid material is obtained at this stage
Method has 3 kinds, when field fructification or the fructification of artificial cultivation, second is that spore, third, liquid fermentation mycelium and fermentation
Liquid.Compared with Ganoderma Lucidum, producing ganodenic acid by liquid deep layer fermenting technology had not by seasonal effect, production week
The features such as phase is short, triterpenes content is stablized relatively, the ready-made most effectual way to obtain ganodenic acid.
With total triterpene contents Feng Changgao in the Ganoderma lucidum mycelium of liquid deep layer fermenting technology production, content, which can reach, is
2~3 times of cultivating ganoderma fructification and lucidum spore powder.It is a kind of with apparent pharmacology in ganoderma lucidum to belong to ganoderic acid A in total triterpene
Activity native compound, be realize strengthen immunity, liver protecting, toxin expelling, blood pressure lowering, norcholesterol, it is antitumor the effects that
Primary efficacy composition.Ganoderic acid A contents are very low in the mycelium and zymotic fluid of fermentation, and content is extremely low, about 5~
10mg/L, content is not high, pharmacological action unobvious.According to the study found that ganoderic acid A has close pass with ganoderma lucidum growth time
System, just can gradually synthesize with the extension ganoderic acid A of time.But when producing Ganoderma lucidum mycelium fermentation by liquid deep layer fermenting technology
Between it is long after, not up to synthesis ganoderic acid A when, automyophagy will be generated, yield is caused to decline, cost increases substantially
Problem.
Invention content
In view of this, the present invention provides it is a kind of improve in liquid fermentation ganoderma lucidum mycelium the fermentation medium of clever acid A and
Fermentation process extends fermentation time, improves the content of ganoderic acid A in glossy ganoderma mycelium fermentation product, strengthens ganoderma lucidum mycelium
Pharmacological activity.
Technical scheme is as follows：
A kind of fermentation medium for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, it is described with weight
The composition of fermentation medium is：Corn flour 2%~5%, analysis for soybean powder 1%~2%, glucose 1%~3%, potassium dihydrogen phosphate
0.1%~0.5%, 200~300 μm of magnesium sulfate 0.05%~0.1%, 50~100ppm of vitamin B1, methyl jasmonate ol/
L, surplus are water.
Preferably, the pH value of the fermentation medium is 6.0~7.0.
The present invention also provides a kind of fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, including following
Step：Ganoderma lucidum mycelium seed liquor is seeded in fermentation medium described in above-mentioned technical proposal and is fermented, the fermentation
Temperature is 25~26 DEG C, when the pH value of fermentation medium is down to 3.5~4.0, terminates fermentation.
Preferably, the time of the fermentation is 3~4 days.
Preferably, the inoculum concentration of the ganoderma lucidum mycelium seed liquor is 10~15%.
Preferably, it is further included after the termination fermentation：Culture medium after fermentation is filtered, crushed after being dried, obtain ganoderma lucidum
Mycelium.
Preferably, the ganoderma lucidum mycelium seed liquor is trains at ganoderma lucidum mycelium strain in liquid medium 25~26 DEG C
It supports and obtains, the fluid nutrient medium is according to mass percentage, including corn flour 2%~4%, analysis for soybean powder 1%~2%, grape
Sugar 1%~3%, yeast extract 1%, potassium dihydrogen phosphate 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, vitamin B1 50~
100ppm, surplus are water.
Preferably, the culture includes activation culture and amplification is cultivated.
It is furthermore preferred that the time of the activation culture is 3~4 days, the period of the amplification culture is 2~3 days.
The invention also includes the ganoderma lucidum mycelium that above-mentioned any one the method is prepared, in the ganoderma lucidum mycelium
The content of ganoderic acid A is 60~105mg/g.
The prior art is compared, and the present invention has the following advantages：
The present invention provides a kind of fermentation medium for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, with weight hundred
Divide content meter, the composition of the fermentation medium is：Corn flour 2%~5%, analysis for soybean powder 1%~2%, glucose 1%~3%,
Potassium dihydrogen phosphate 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, 50~100ppm of vitamin B1, methyl jasmonate 200
~300 μm of ol/L, surplus are water.It is added to a certain amount of methyl jasmonate in the fermentation medium, optimization culture based formulas,
The fermentation time of ganoderma lucidum mycelium can be extended, mycelia yield is made to ensure the generated time of ganoderic acid A in the case of not reducing, improved
The content of ganoderic acid A in tunning.
A kind of fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium provided by the invention, with containing jasmonic
The culture medium of methyl esters carries out liquid deep layer fermenting to ganoderma lucidum mycelium, glossy ganoderma fermentation incubation time is made to extend to 3~4 days, mycelia
It is further cultured for after self-dissolving, allows and largely synthesized during ganoderic acid A secondary metabolisms, form the ingredient of ganoderma lucidum mycelium structure.It produces in this way
Ganoderic acid A contents are up to 60~105mg/L in the ganoderma lucidum mycelium come, and the efficient medical value exploitation for ganoderma lucidum provides one kind
Approach.And production process is simple controllable, it can be achieved that batch production.
Description of the drawings
Fig. 1 is the mycelial fermentation process of fluid present invention fermentation glossy ganoderma.
Specific embodiment
The present invention provides a kind of fermentation medium for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, with weight hundred
Divide content meter, the composition of the fermentation medium is：Corn flour 2%~5%, analysis for soybean powder 1%~2%, glucose 1%~3%,
Potassium dihydrogen phosphate 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, 50~100ppm of vitamin B1, methyl jasmonate 200
~300 μm of ol/L, surplus are water.
In the present invention, the composition of the fermentation medium is preferably：Corn flour 3%~4%, analysis for soybean powder 1.2%~
1.8%th, glucose 1.5%~2.5%, potassium dihydrogen phosphate 0.2%~0.4%, magnesium sulfate 0.06%~0.08%, vitamin B1
220~270 μm of 60~90ppm, methyl jasmonate ol/L, surplus is water.
In the present invention, the pH of the fermentation medium is preferably 6.0~7.0, and more preferably 6.5~7.0.
A certain amount of methyl jasmonate is added in the fermentation medium of the present invention, it, can by optimization culture based formulas
Extend the fermentation time of ganoderma lucidum mycelium, mycelia yield is made to ensure the generated time of ganoderic acid A in the case of not reducing, improve fermentation
The content of ganoderic acid A in product.
The component of fermentation medium of the present invention according to aforementioned proportion is mixed, liquid fermentation ganoderma lucidum is prepared
Mycelial fermentation medium.
The present invention also provides a kind of fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, including following
Step：Ganoderma lucidum mycelium seed liquor is seeded in fermentation medium described in above-mentioned technical proposal and is fermented, the fermentation
Temperature is 25~26 DEG C, when the pH value of fermentation medium is down to 3.5~4.0, terminates fermentation.
Ganoderma lucidum mycelium seed liquor of the present invention is trains at ganoderma lucidum mycelium strain in liquid medium 25~26 DEG C
It supports and obtains, the Liquid Culture that the fluid nutrient medium is obtained for the plating medium of preservation ganoderma lucidum mycelium strain after dilution
Base.Fluid nutrient medium of the present invention is according to mass percentage, including corn flour 2%~4%, analysis for soybean powder 1%~2%, Portugal
Grape sugar 1%~3%, yeast extract 1%, potassium dihydrogen phosphate 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, vitamin B1 50
~100ppm, surplus are water；Preferably corn flour 2.5%~3.5%, analysis for soybean powder 1.5%, glucose 1.5%, yeast extract 1%,
Potassium dihydrogen phosphate 0.2%~0.4%, magnesium sulfate 0.06%~0.08%, 60~90ppm of vitamin B1, surplus are water.It is described
Culture medium of the plating medium for the conventional ganoderma lucidum mycelium preservation in this field, such as PDA culture medium.
The present invention is not particularly limited the source of ganoderma lucidum mycelium strain, using the conventional preservation in this field or commercially available
Ganoderma lucidum mycelium strain.
In the present invention, the culture preferably includes the activation culture carried out successively and amplification is cultivated.The activation culture and
Amplification culture culture medium used is preferably the fluid nutrient medium described in above-mentioned technical proposal.
Activation culture of the present invention is that the plating medium with ganoderma lucidum mycelium is diluted with water to be inoculated in liquid training
Support in base, the diluted multiple be preferably 1 square centimeter or so of band culture medium flat plate strain dilution be seeded to 100~
In 300ml fluid nutrient mediums；More preferably 1 square centimeter or so of band culture medium flat plate strain dilution is seeded to 150ml liquid
In culture medium.By the fluid nutrient medium shake culture after dilution.The speed of the concussion is 100~120 turns/min, more preferably
110 turns/min.The time of the activation culture is preferably 3~4 days.Activation culture of the present invention preferably in agitator tank into
Row, shake culture is carried out by the agitator tank in an oscillator.Specifically, agitator tank of the present invention is preferably 30L agitator tanks.
After shake culture, the ganoderma lucidum mycelium that is activated.
The ganoderma lucidum mycelium of activation is amplified culture.It is of the present invention amplification culture be preferably level-one amplification culture and
Two level amplification culture.The amplification culture carries out preferably in fermentation tank, is carried out from small to large step by step according to the volume of fermentation tank
Amplification, the present invention preferably level-one amplify culture using 300~500L seeding tanks, and two level amplification culture uses 1~2 ton of seed
Tank.The period of preferably every grade amplification culture of the present invention is preferably 2~3 days.After fermentation 2~3 days, the mycelia in fluid nutrient medium
Measure it is excessive, the nutrition that fluid nutrient medium is provided cannot support mycelia grow needed for, so as to occur " aging " mycelia from
It is dissolved in culture solution, mycelia is caused to propose rate decline.Therefore, amplification culture terminates hair before there is ganoderma lucidum mycelium autolysis
Ferment.After amplification culture, obtained culture solution is ganoderma lucidum mycelium seed liquor.
Ganoderma lucidum mycelium seed liquor is inoculated in the fermentation medium of the present invention and is fermented.The inoculum concentration is preferably
10~15%, more preferably 12~13%.Fermentation of the present invention is aerobic fermentation.Ventilation ratio in the fermentation process is preferred
It is 0.5~1.0, more preferably 0.6~0.8；Stir speed (S.S.) in the fermentation process is 100~120 turns/min, more preferably
110 turns/min.
With the progress of fermentation, the pH value of fermentation medium is gradually reduced, preferably when the pH value of fermentation medium is down to 3.8
When~3.9, stop fermentation.At this point, the fermentation time of ganoderma lucidum mycelium is preferably 3~4 days.And routine does not add methyl jasmonate
Fluid nutrient medium, there is autolysis less than 3 days to the fermentation time of ganoderma lucidum mycelium, no time enough caused to exist
Ganoderic acid A is synthesized in ganoderma lucidum mycelium, the content of ganoderic acid A is very low in tunning.
After the present invention terminates fermentation, the fermentation medium with ganoderma lucidum mycelium is taken out, filtering, crushed after being dried obtain
To ganoderma lucidum mycelium product.Filtering of the present invention is preferably plate compression, and the pressure is preferably 50~60kg, more preferably
55kg.Drying of the present invention is preferably dried in vacuo, and the vacuum drying temperature is preferably 60~65 DEG C, more preferably 62
~64 DEG C.The grain size of crushing of the present invention is preferably 60~80 mesh, more preferably 65~70 mesh.
In the fermentation glossy ganoderma mycelium that the above-mentioned fermentation process of the present invention obtains, the content of ganoderic acid A can reach 60~
105mg/g substantially increases the pharmaceutical activity of ganoderma lucidum mycelium, improve its strengthen immunity, liver protecting, toxin expelling, blood pressure lowering,
Norcholesterol, it is antitumor the effects that effect.
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiment to the present invention into
Row detailed description, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
1) ganoderma lucidum mycelium strain, is chosen：
2), shaking table kind：25 DEG C are maintained the temperature at, 300ml is diluted to 1 square centimeter or so of band culture medium flat plate strain
It in fluid nutrient medium, is placed on oscillator, and stirs 3 days, control mixing speed is 100 turns/min；The fluid nutrient medium packet
Include corn flour 2%, analysis for soybean powder 1%, glucose 1%, yeast extract 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, vitamin
B1 50ppm, surplus are water.
3), level-one is amplified：25 DEG C are maintained the temperature at, the culture medium negative pressure after stirring in the step 2) is sucked into a 300L
In seeding tank with fluid nutrient medium, cultivate 2 days；
4), two level is amplified：25 DEG C are maintained the temperature at, by the fluid nutrient medium negative pressure sucking one after stirring in the step 3)
In 1.5 tons of seeding tanks with fluid nutrient medium, cultivate 2 days, obtain ganoderma lucidum mycelium seed liquor；
5), methyl jasmonate culture medium ferments：25 DEG C are maintained the temperature at, the ganoderma lucidum mycelium that will be obtained in the step 4)
Seed liquor 1000L negative pressure is sucked in one 7 tons of fermentation tank, and adds in the fermentation that 5000L contains methyl jasmonate (254 μm of ol/L)
In culture medium, ferment 3 days；
Score meter by weight, the composition of the methyl jasmonate culture medium are：Corn flour 2%, analysis for soybean powder 1%, glucose
1%th, 254 μm of potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, vitamin B1 50ppm, methyl jasmonate ol/L, surplus is goes
Ionized water.
The pH value of fluid nutrient medium after fermentation is 3.8.
6), plate compression：Fluid nutrient medium after the step 5) is fermented takes out, and with the pressure press filtration of 50kg, press dry,
Obtain solid head product；
7), drying and crushing：The solid head product that the step 6) obtains is placed in vacuum to dry, drying temperature 60
DEG C, it is crushed after the completion of dry, obtains the high-content ganoderic acid A ganoderma lucidum mycelium products that grain size is 60 mesh, yield 2%.
Embodiment 2
1) ganoderma lucidum mycelium strain, is chosen：
2), shaking table kind：26 DEG C are maintained the temperature at, 150ml is diluted to 1 square centimeter or so of band culture medium flat plate strain
It in fluid nutrient medium, is placed on oscillator, and stirs 4 days, control mixing speed is 120 turns/min；The fluid nutrient medium is pressed
According to mass percentage, including corn flour 4%, analysis for soybean powder 2%, glucose 1%~3%, yeast extract 1%, potassium dihydrogen phosphate
0.5%th, magnesium sulfate 0.1%, vitamin B1 100ppm, surplus are water.
3), level-one is amplified：26 DEG C are maintained the temperature at, the culture medium negative pressure after stirring in the step 2) is sucked into a 300L
In seeding tank with fluid nutrient medium, cultivate 3 days；
4), two level is amplified：26 DEG C are maintained the temperature at, by the fluid nutrient medium negative pressure sucking one after stirring in the step 3)
In 1.5 tons of seeding tanks with fluid nutrient medium, cultivate 3 days, obtain ganoderma lucidum mycelium seed liquor；
5), methyl jasmonate culture medium ferments：26 DEG C are maintained the temperature at, the ganoderma lucidum mycelium that will be obtained in the step 4)
Seed liquor 1000L negative pressure is sucked in one 7 tons of fermentation tank, and adds in the fermentation that 5000L contains methyl jasmonate (300 μm of ol/L)
In culture medium, ferment 3 days；
Score meter by weight, the composition of the methyl jasmonate culture medium are：Corn flour 5%, analysis for soybean powder 2%, glucose
3%th, 300 μm of potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.1%, vitamin B1 100ppm, methyl jasmonate ol/L, surplus is goes
Ionized water.
The pH value of fluid nutrient medium after fermentation is 4.0.
6), plate compression：Fluid nutrient medium after the step 5) is fermented takes out, and with the pressure press filtration of 60kg, press dry,
Obtain solid head product；
7), drying and crushing：The solid head product that the step 6) obtains is placed in vacuum to dry, drying temperature 65
DEG C, it is crushed after the completion of dry, obtains the high-content ganoderic acid A ganoderma lucidum mycelium products that grain size is 80 mesh, yield 2.3%.
Embodiment 3
1) ganoderma lucidum mycelium strain, is chosen：
2), shaking table kind：26 DEG C are maintained the temperature at, 200ml is diluted to 1 square centimeter or so of band culture medium flat plate strain
It in fluid nutrient medium, is placed on oscillator, and stirs 3 days, control mixing speed is 110 turns/min；The fluid nutrient medium is pressed
According to mass percentage, including corn flour 3%, analysis for soybean powder 2%, glucose 2%, yeast extract 1%, potassium dihydrogen phosphate 0.3%, sulphur
Sour magnesium 0.08%, vitamin B1 75ppm, surplus are water.
3), level-one is amplified：25 DEG C are maintained the temperature at, the culture medium negative pressure after stirring in the step 2) is sucked into a 300L
In seeding tank with fluid nutrient medium, cultivate 2 days；
4), two level is amplified：25 DEG C are maintained the temperature at, by the fluid nutrient medium negative pressure sucking one after stirring in the step 3)
In 1.5 tons of seeding tanks with fluid nutrient medium, cultivate 2 days, obtain ganoderma lucidum mycelium seed liquor；
5), methyl jasmonate culture medium ferments：26 DEG C are maintained the temperature at, the ganoderma lucidum mycelium that will be obtained in the step 4)
Seed liquor 1000L negative pressure is sucked in one 7 tons of fermentation tank, and adds in the fermentation that 5000L contains methyl jasmonate (200 μm of ol/L)
In culture medium, ferment 3 days；
Score meter by weight, the composition of the methyl jasmonate culture medium are：Corn flour 4%, analysis for soybean powder 2%, glucose
2%th, 300 μm of potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.07%, vitamin B1 80ppm, methyl jasmonate ol/L, surplus is goes
Ionized water.
The pH value of fluid nutrient medium after fermentation is 3.9.
6), plate compression：Fluid nutrient medium after the step 5) is fermented takes out, and with the pressure press filtration of 60kg, press dry,
Obtain solid head product；
7), drying and crushing：The solid head product that the step 6) obtains is placed in vacuum to dry, drying temperature 63
DEG C, it is crushed after the completion of dry, obtains the high-content ganoderic acid A ganoderma lucidum mycelium products that grain size is 70 mesh, yield 2.1%.
Comparative example
Other steps are such as embodiment 1, only above-mentioned steps 5) in fermentation medium in do not contain methyl jasmonate.Step
There is ganoderma lucidum mycelium autolysis when rapid 5) fermentation was to the 3rd day.Before ganoderma lucidum mycelium self-dissolving, obtained ganoderma lucidum mycelium product
Yield is up to 1.3%.
Embodiment 4
Detect the content of ganoderic acid A in the ganoderma lucidum mycelium of Examples 1 to 3
Ganoderic acid detection method is as follows：
1st, ganoderic acid A samples：
Ganoderic acid A (ganoderic acidA) reference substance is purchased in market, and mass fraction is in terms of 95.0%.
2nd, the preparation of reference substance solution
Precision weighs the ganoderic acid A11.16mg being dried under reduced pressure to constant-quality, puts in 25ml volumetric flasks, methanol is added to dissolve
And scale is diluted to, it shakes up up to the reference substance solution that mass concentration is 446.4 μ g/mL.
3rd, the preparation of sample solution is detected
Ganoderma lucidum mycelium powder is taken to crushed No. 1 sieve.1g is taken, it is accurately weighed, it puts in conical flask with cover, adds methanol solution 25ml,
It is ultrasonically treated 2 times, each 40min, filtration, merging filtrate is put and is evaporated on water-bath.Residue adds methanol to dissolve, and is transferred to 5mL appearances
In measuring bottle, be settled to scale, shake up, take subsequent filtrate, 0.45 μm of miillpore filter filter to get.
4th, chromatographic condition
Chromatographic column uses WelchXtimateTM- 18C chromatographic columns (4.6mm × 250mm, 5 μm) mobile phase is acetonitrile-volume
Score 0.01% acetic acid (volume ratio 37：63) flow velocity be 1mL/min, column temperature be 30 DEG C, sample size be 10 μ L, evaporative light-scattering device
Drift tube temperature is 105 DEG C, air velocity 2.8L/min,.It is accurate respectively to draw blank solvent methanol, reference substance, test sample
10 μ L of solution inject high performance liquid chromatograph, and gained HPLC chromatogram measures ganoderic acid A contents.It the results are shown in Table 1：
The content of ganoderic acid A in the ganoderma lucidum mycelium of 1 Examples 1 to 3 of table
| Content (mg/g) | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Ganoderic acid A | 98.2 | 101.5 | 72.6 | 31.3 |
It can be seen from Table 1 that fermentation medium of the invention can extend the fermentation time of ganoderma lucidum mycelium, make mycelia
Yield ensures the generated time of ganoderic acid A in the case of not reducing, improve the content of ganoderic acid A in tunning.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of fermentation medium for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, which is characterized in that contained with weight percent
Gauge, the composition of the fermentation medium are：Corn flour 2%~5%, analysis for soybean powder 1%~2%, glucose 1%~3%, phosphoric acid
Potassium dihydrogen 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, 0~100ppm of pangamic acid, 200~300 μ of methyl jasmonate
Mol/L, surplus are water.
2. fermentation medium according to claim 1, which is characterized in that the pH value of the fermentation medium for 6.0~
7.0。
3. a kind of fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium, which is characterized in that include the following steps：
Ganoderma lucidum mycelium seed liquor is seeded in fermentation medium described in claims 1 or 2 and is fermented, the temperature of the fermentation is
25~26 DEG C, when the pH value of fermentation medium is down to 3.5~4.0, terminate fermentation.
4. according to the method described in claim 3, it is characterized in that, the time of the fermentation is 3~4 days.
5. according to the method described in claim 3, it is characterized in that, the inoculum concentration of the ganoderma lucidum mycelium seed liquor for 10~
15%.
6. it according to the method described in claim 3, it is characterized in that, is further included after the termination fermentation：By the culture after fermentation
Base filtering, crushed after being dried, obtain ganoderma lucidum mycelium.
7. the method according to claim 3 or 5, which is characterized in that the ganoderma lucidum mycelium seed liquor is ganoderma lucidum mycelium
Strain is cultivated at 25~26 DEG C obtain in liquid medium, and the fluid nutrient medium is according to mass percentage, including corn
Powder 2%~4%, analysis for soybean powder 1%~2%, glucose 1%~3%, yeast extract 1%, potassium dihydrogen phosphate 0.1%~0.5%, sulphur
Sour magnesium 0.05%~0.1%, 0~100ppm of pangamic acid, surplus are water.
8. the method according to the description of claim 7 is characterized in that the culture includes activation culture and amplification is cultivated.
9. according to the method described in claim 8, it is characterized in that, the time of the activation culture be 3~4 days, the amplification
The period of culture is 2~3 days.
10. the ganoderma lucidum mycelium being prepared by claim 3~9 any one the method, which is characterized in that the ganoderma lucidum
The content of ganoderic acid A is 60~105mg/g in mycelium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810332406.8A CN108251490B (en) | 2018-04-13 | 2018-04-13 | Fermentation medium and fermentation method for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810332406.8A CN108251490B (en) | 2018-04-13 | 2018-04-13 | Fermentation medium and fermentation method for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108251490A true CN108251490A (en) | 2018-07-06 |
| CN108251490B CN108251490B (en) | 2020-08-04 |
Family
ID=62748284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810332406.8A Active CN108251490B (en) | 2018-04-13 | 2018-04-13 | Fermentation medium and fermentation method for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108251490B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913730A (en) * | 2018-07-10 | 2018-11-30 | 长江大学 | A kind of cultural method of Monascus high yield monascus purpureus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000319287A (en) * | 1999-05-11 | 2000-11-21 | Meiji Seika Kaisha Ltd | New triterpene and its production |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
-
2018
- 2018-04-13 CN CN201810332406.8A patent/CN108251490B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000319287A (en) * | 1999-05-11 | 2000-11-21 | Meiji Seika Kaisha Ltd | New triterpene and its production |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
Non-Patent Citations (2)
| Title |
|---|
| 苗长海等: "《灵芝高效栽培技术》", 31 March 2002, 河南科学技术出版社 * |
| 辛燕花等: "外源茉莉酸甲酯对灵芝多糖及灵质酸含量的影响", 《山西农业大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913730A (en) * | 2018-07-10 | 2018-11-30 | 长江大学 | A kind of cultural method of Monascus high yield monascus purpureus |
| CN108913730B (en) * | 2018-07-10 | 2021-09-28 | 长江大学 | Method for culturing high-yield lovastatin by using monascus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108251490B (en) | 2020-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103740772B (en) | A kind of method promoting camphor tree sesame liquid state fermentation biosynthesizing Android tonquinol | |
| CN107227264B (en) | A kind of Lasiodiplodia iranensis bacterium and its method for producing jasmonic of fermenting | |
| CN106520562A (en) | Method for cultivating caterpillar fungus by using ginseng type extract and application | |
| CN112501029A (en) | Armillaria matsutake and method for producing ergothioneine by using same | |
| CN112574893A (en) | Phellinus baumii, and preparation method and application of fermentation product thereof | |
| CN110004066A (en) | A kind of mystery Ganoderma Varieties By Uv Induced and its artificial cultivation method and application | |
| CN102925527A (en) | Method for mixing and fermenting flammulina velutipes and lucid ganoderma | |
| CN109055241A (en) | A method of preparing mortierella Diding culture | |
| CN106715708A (en) | Process for preparing carotenoids by submerged fermentation with mixed cultures of (+) and (-) strains of the fungus blakeslea trispora | |
| CN105018350A (en) | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium | |
| CN110257261A (en) | A kind of production method of selenium-rich antrodia mycelia | |
| CN110004065A (en) | A kind of reddish brown Ganoderma Varieties By Uv Induced and its artificial cultivation method and application | |
| CN108251490A (en) | A kind of fermentation medium and fermentation process for improving ganoderic acid A in liquid fermentation ganoderma lucidum mycelium | |
| JP4227474B2 (en) | Culture method of Meshimakobu | |
| CN108719292A (en) | A kind of special synergistic agent of beauveria bassiana class biological pesticide and the preparation method and application thereof | |
| CN105255948B (en) | The preparation method of Inonotus obliquus zymotic fluid and the zymotic fluid are preparing the application in reducing blood glucose health beverages | |
| TWI385248B (en) | A formula of culturing medium for cordyceps spp. | |
| CN106922386A (en) | A kind of artificial culture method of cicada fungus | |
| CN108486180A (en) | A kind of fermentation preparation of oosporein | |
| CN102618604B (en) | Preparation method of cyclic lipopeptide compound | |
| CN101407767A (en) | Method for producing Chinese caterpillar fungus by fermentation | |
| JPH04304890A (en) | Production of ganoderic acids | |
| CN108841891B (en) | Method for reducing capacity of monascus to produce citrinin through liquid and solid state fermentation by rutin derivatives | |
| Zhu et al. | Fluid flow induced shear stress affects cell growth and total flavone production by Phellinus igniarius in stirred-tank bioreactor | |
| CN108624509A (en) | The circulation utilization method of solid medium in a kind of artificial incubation of cicada fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |