CN107227264B - A kind of Lasiodiplodia iranensis bacterium and its method for producing jasmonic of fermenting - Google Patents
A kind of Lasiodiplodia iranensis bacterium and its method for producing jasmonic of fermenting Download PDFInfo
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Abstract
The invention discloses a kind of methods that Lasiodiplodia iranensis bacterium and its fermentation produce jasmonic, belong to field of biotechnology.The present invention provides one plant of bacterial strain DWH-2 that can produce jasmonic, is accredited as Lasiodiplodia iranensis through ITS, which is preserved in China typical culture collection center, deposit number on May 27th, 2017 are as follows: CCTCC NO:M2017288.DWH-2 is cultivated 4~5 days on solid medium, and static fermentation 6~10 days, can produce the jasmonic of 1500mg/L or more in fermentation medium of transferring.The present invention provides a kind of methods for producing jasmonic with microbe fermentation method, compared with chemical synthesis process, have very big advantage.
Description
Technical field
The present invention relates to a kind of methods that Lasiodiplodia iranensis bacterium and its fermentation produce jasmonic, belong to life
Object technical field.
Background technique
Jasmonic chemical name 3- oxygen -2-2 '-cis- pentenyl-pentamethylene -1- acetic acid, molecular formula C12H18O3, molecular weight
210.27.It is a kind of novel plant hormone, it is generally existing in the tissues such as the flower of plant, stem, leaf, root and organ, in plant
Growth and development process in play an important role.It is used as second signal simultaneously, in plant by biology and abiotic wound
Induced activation plant intracorporal defensin gene when evil resists extraneous injury.And because jasmonic and its methyl esters are with light, deep and remote
Refined fragrance, so its ingredient that can be used as many fragrant flower essential oil main notes, is widely used in the generation of perfumery.In agricultural
In production, jasmonate, which can significantly promote sterile plant to bloom, can also be used to improve the drought resistance of plant, while jasmine
Jasmine acid can induce plant to generate noxious material, pest protease inhibitors etc. to achieve the effect that insect pest, in agriculture
It can use it to replace part insecticide in industry production.
It at present there are mainly three types of the preparation methods of jasmonic, is extracted from plants, chemical synthesis and microbe fermentation method.It plants
Jasmine acid content is 1 in object-9~10-6G/g, therefore, the amount being extracted from plants are few, can not meet current demand.Chemical method
According to starting material difference, it is divided into that cis- 4- heptenoic acid is starting material, ethyl acetate has been starting material and 2- cyclopentene -1-
Ethylene ketal and 2- methyl-1,3- butadiene be starting material method, but the reaction yield of these methods it is not high (30% with
Under), severe reaction conditions, and the jasmonic synthesized is all racemate, limits its range used.
Microbe fermentation method is to produce jasmonic by fungi fermentation.Aldridge etc. is earliest from fungi Lasiodiplodia theobromae
Free jasmonic (J.Chem Soc Chem Coummum. is isolated in Lasiodiplodia theobroma culture solution
1971,13:1623), this illustrates that certain micro-organisms has the possibility of secretion jasmonic.United States Patent (USP) US20020110881A1 is public
The method that two spore of cotton color (Diplodiagossypina) fermentation produces jasmonic, methyl jasmonate and jasmine acid isomer has been opened,
In, D.gossypina ATCC 10936 produces jasmine acid concentration up to 1.2g/L.The country, Han Xiaomin etc. are detection cocoa hair color
Two spore bacterium generate the inducing action of sequiterpene to suspension culture of Aquilaria sinensis, detect Jasmonates in its PDA culture solution using gas chromatography mass spectrometry
Compound (Chinese traditional Chinese medicine magazine, 2014,39 (2): 192-196), but wherein methyl jasmonate content is not high (250mg/L),
Simultaneously without the research in terms of the related fermenting and producing of progress.
Summary of the invention
The invention solves first technical problem be to provide one plant of Lasiodiplodia iranensis DWH-2,
It can produce jasmonic.The present invention is to rotten fruits and vegetables and Yu Rui, the corruption for drawing the plants such as tribute wood, Hai Sang, Indian beech, corn, cotton
Microorganism on rotten position is separated, and screening obtains the bacterial strain DWH-2 of secretion jasmonic, belongs to one plant through Molecular Identification
L.iranensis bacterium, yield are apparently higher than general wild strain, are further increased by Optimal Medium and condition of culture
Production concentration.The L.iranensis DWH-2 is preserved in China typical culture collection on May 27th, 2017
The heart, deposit number are CCTCC NO:M2017288.
Bacterial strain can be connected on PDA plate by the activation culture of the DWH-2, be placed in 25~28 DEG C of incubator living
Change 4~5 days.
The DWH-2 is on PDA plate, and bacterium colony early growth period is white, and day growth amount is very fast, when growing into the 4th day
The plate back side starts green occur, and culture medium begins to change into black when growing into the 6th day, and culture medium is complete when growing into the 9th day
Become black entirely.In electric microscopic observation, it is found that the mycelium of the bacterium has tabula, and be multicore.
The ITS gene order similitude of the ITS gene order of the DWH-2 and more plants of L.iranensis up to 99%~
100%, but these L.iranensis are not about the report for producing jasmonic.
The invention solves second technical problem be to provide a kind of application L.iranensis DWH-2 fermentation and produce
The method of jasmonic.
It ferments using the L.iranensis DWH-2 and produce jasmonic, is carried out under static gas wave refrigerator state.It is included in 20
~30 DEG C, static fermented and cultured 6~10 days.
For the L.iranensis DWH-2 ferment produce jasmonic fermentation medium carbon source include sucrose, starch,
The mixing of one or more of maltose, glucose, fructose.Carbon source concentration is 25~500g/L.
The inorganic nitrogen-sourced of the fermentation medium for producing jasmonic of fermenting for the L.iranensis DWH-2 includes nitric acid
The mixing of one or more of sodium, urea, ammonium nitrate.Inorganic nitrogen-sourced concentration is 3~15g/L.
Producing the organic nitrogen source of the fermentation medium of jasmonic for L.iranensis DWH-2 fermentation includes beef
The mixing of one or more of cream, peptone, corn pulp, yeast extract, malt extract.Organic nitrogen source concentration is 3~15g/
L。
In one embodiment of the invention, it ferments for the L.iranensis DWH-2 and produces the fermentation of jasmonic
Culture medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, 0.5~3g/L of potassium dihydrogen phosphate, 0.1~0.4g/L of potassium chloride,
1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, corn pulp 1~5g/L, 0.2-20g/L soybean oil, micro member
Element 10~12ml/L, pH 4.5~8；The microelement: 0.01~0.04g/L of green-vitriol, seven water manganese sulfates 0.001
~0.004g/L, seven brochanite 0.001~0.004g/L, two 0.001~0.004g/L of molybdic acid hydrate sodium.
During application L.iranensisDWH-2 fermentation produces jasmonic, macropore can also be added into fermentation system
Resin.The model of macroreticular resin includes XAD-1, XAD-2, XAD-7HP, AB-8, D101.Dosage is 5~20g/100mL.
It ferments using L.iranensisDWH-2 provided by the invention and produces jasmonic, the yield of jasmonic can reach
1760mg/L。
Biomaterial preservation
Lasiodiplodia iranensisDWH-2 has been preserved in Chinese Typical Representative culture guarantor on May 27th, 2017
Hiding center, deposit number are CCTCC NO:M2017288, and preservation address is the Wuhan Wuhan University, China.
Detailed description of the invention
Fig. 1 TLC product Preliminary Identification figure
Fig. 2 product identifies GC-MS figure
The chadogram of Fig. 3 bacterial strain DWH-2
Specific embodiment
Product analysis method:
Initial characterization is carried out to product using thin-layer chromatography (TLC).Reference literature method, lamellae used are GF254,
Solvent are as follows: n-hexane: ethyl acetate: acetic acid=60:40:1, color developing agent are the concentrated sulfuric acid: methanol=1:1
Product is further identified with gas chromatography mass spectrometry (GC-MS).Reference literature method.Makings condition: gas phase color
30m × 0.25mm × 0.25 μm column AB--5MS is composed, carrier gas is helium, and pressure 130kg/cm2, dividing meter pressure is 0. 31kg/
Cm2, flow velocity 0.8mL/min.Chromatographic column use temperature programming, from 50 DEG C of initial temperature rise to 180 DEG C with 20 DEG C/min after keep
4min, then risen to after 290 DEG C with 10 DEG C/min and keep 15min, 250 DEG C of sample introductions do not shunt, 1 μ L of sampling volume.
The concentration of product is detected with HPLC (high performance liquid chromatography), reference literature method.Mobile phase is methanol:
Water: phosphoric acid=55:44.9:0.1；10~30uL of sample volume；Flow velocity 1mL/min；Detector is UV detector.
The screening and determination of the production jasmonic bacterial strain of embodiment 1
Fermentation medium: 25~500g/L of sucrose, 1~15g/L of sodium nitrate, 0.5~3g/L of potassium dihydrogen phosphate, potassium chloride
0.1~0.4g/L, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, corn pulp 1~5g/L, 0.2-20g/L are big
Soya-bean oil, microelement 10~12ml/L, pH 4.5~8.The microelement: 0.01~0.04g/L of green-vitriol, seven water
Manganese sulfate 0.001~0.004g/L, seven brochanite 0.001~0.004g/L, two 0.001~0.004g/L of molybdic acid hydrate sodium.
With rotten fruit and Yu Rui, draw the rotten position of the plants such as tribute wood, Hai Sang, Indian beech, corn, cotton for sieve bacterium
Material has been processed to dilution spread in PDA plate, and 25 DEG C~30 DEG C are cultivated 4~7 days.The bacterium grown is connected to punch
500 ml ferment 6~10 days in the static incubator for being placed on 20~30 DEG C equipped in the triangular flask of fermentation medium.Fermentation knot
Supernatant is taken after being centrifuged fermentation liquid after beam, its pH is adjusted to 3~4 or so with the hydrochloric acid of 6mol/L, then fermented with 0.5~5 times
The ethyl acetate extraction of liquid product, ethyl acetate is evaporated off with vacuum rotary evaporator after extraction, product is dissolved in methanol.
Preliminary qualitative detection is carried out with TLC (thin-layer chromatography), the fermentation liquid (Fig. 1) for having punctation to occur is subjected to HPLC into one
The detection of step has seen whether that peak identical with standard specimen appearance time occurs.Sample GC-MS identical with standard specimen appearance time
(gas chromatography mass spectrometry) is identified, and the concentration mensuration of product is carried out with HPLC (high performance liquid chromatography), and GC-MS figure is shown in attached drawing 2.
It is screened, obtains one plant of bacterial strain DWH-2 that can produce jasmonic, the concentration for producing jasmonic reaches 320mg/L fermentation
Liquid.
After DWH-2 is cultivated on PDA plate carry out ITS sequencing, measure ITS gene order (SEQ ID NO:1) with
The ITS gene order similitude of Lasiodiplodia iranensis is up to 100%.DWH-2 is on PDA plate, and bacterium colony growth is just
Phase is white, and day growth amount is very fast, and the plate back side starts green, culture when growing into the 6th day occur when growing into the 4th day
Base begins to change into black, and culture medium becomes black completely when growing into the 9th day.In electric microscopic observation, the mycelium of the bacterium is found
There is tabula, and is multicore.Therefore, through morphology and molecular biology identification, DWH-2 is Lasiodiplodia iranensis.
2 training method of embodiment produces the influence of jasmonic to bacterial strain
Fermentation medium: 25~500g/L of sucrose, 1~15g/L of sodium nitrate, 0.5~3g/L of potassium dihydrogen phosphate, potassium chloride
0.1~0.4g/L, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, corn pulp 1~5g/L, 0.2-20g/L are big
Soya-bean oil, microelement 10~12ml/L, pH 4.5~8.Microelement: 0.01~0.04g/L of green-vitriol, seven water sulfuric acid
Manganese 0.001~0.004g/L, seven brochanite 0.001~0.004g/L, two 0.001~0.004g/L of molybdic acid hydrate sodium.
The bacterium grown is connected to 500ml with punch to be equipped in the triangular flask of fermentation medium, in 25~3 DEG C of incubator
Three kinds of different training methods, static gas wave refrigerator, reciprocal shaker training is arranged in middle fermented and cultured 6~10 days during culture
Feeding and rotary shaker culture.Fermented supernatant fluid surveys the concentration of product with HPLC, as a result such as table 1.
1 training method of table produces the influence of jasmonic to bacterial strain
| Training method | Production concentration (mg/L) |
| Stationary culture | 342 |
| Reciprocal shaker | 56 |
| Rotary shaker | 62 |
3 different carbon source of embodiment produces the influence of jasmonic to bacterial strain
Static gas wave refrigerator is carried out to DWH-2 as described in Example 2, wherein the carbon source (50g/L) of fermentation medium is respectively
Sucrose, lactose, starch, maltose, glucose, fructose, in the incubator 25~30 DEG C static gas wave refrigerator 6~10 days, fermentation supernatant
Liquid surveys the concentration of product with HPLC, as a result such as table 2.
2 different carbon source of table produces the influence of jasmonic to bacterial strain
| Carbon source | Production concentration (mg/L) |
| Sucrose | 314 |
| Lactose | 31 |
| Starch | 140 |
| Maltose | 258 |
| Glucose | 199 |
| Fructose | 102 |
4 carbon source various concentration of embodiment produces the influence of jasmonic to bacterial strain
With 2 method of embodiment, wherein fermentation medium is using sucrose as sole carbon source, and the concentration that sucrose is arranged is respectively
25g/L, 50g/L, 75g/L, 100g/L, 125g/L, 150g/L, 300g/L, 500g/L 25~30 DEG C of static trainings in the incubator
It supports 6~10 days.HPLC surveys the concentration of the product in fermentation liquid, as a result such as table 3.
Influence of the 3 carbon source various concentration of table to jasmonic is produced
| Sucrose concentration (g/L) | Production concentration (mg/L) |
| 25 | 310 |
| 50 | 517 |
| 75 | 267 |
| 100 | 238 |
| 125 | 177 |
| 150 | 185 |
| 300 | 189 |
| 500 | 201 |
The inorganic nitrogen-sourced influence that jasmonic is produced to bacterial strain of embodiment 5
As described in Example 2, wherein fermentation medium using sucrose as sole carbon source, it is inorganic nitrogen-sourced be respectively sodium nitrate,
Urea, ammonium nitrate, ammonium chloride, ammonium sulfate, in the incubator 25~30 DEG C static gas wave refrigerator 6~10 days.The concentration of HPLC survey product
Such as table 4.
The inorganic nitrogen-sourced influence to jasmonic is produced of table 4
| It is inorganic nitrogen-sourced | Production concentration (mg/L) |
| Sodium nitrate | 224 |
| Urea | 116 |
| Ammonium nitrate | 3.6 |
| Ammonium chloride | 0.503 |
| Ammonium sulfate | 0.45 |
The inorganic nitrogen concentration of embodiment 6 produces the influence of jasmonic to bacterial strain
As described in Example 2, wherein for fermentation medium using sucrose as sole carbon source, inorganic nitrogen-sourced is sodium nitrate, setting
The concentration of sodium nitrate be 3g/L, 4.5g/L, 6g/L, 7.5g/L, 9g/L, 10.5g/L, 15g/L in the incubator 25~30 DEG C it is quiet
Only cultivate 6~10 days.The concentration such as table 5 of HPLC survey product.
Influence of the inorganic nitrogen concentration of table 5 to jasmonic is produced
| Sodium nitrate concentration (g/L) | Production concentration (mg/L) |
| 3 | 793 |
| 4.5 | 805 |
| 6 | 709 |
| 7.5 | 447.4 |
| 9 | 660.4 |
| 10.5 | 399.5 |
| 15 | 380.9 |
7 organic nitrogen source of embodiment produces the influence of jasmonic to bacterial strain
As described in Example 2, wherein for fermentation medium using sucrose as sole carbon source, inorganic nitrogen-sourced is sodium nitrate, organic
Nitrogen source is beef extract, peptone, corn pulp, yeast extract, malt extract, in the incubator 25~30 DEG C of static gas wave refrigerators 6~10
It.The concentration such as table 6 of HPLC survey product.
6 organic nitrogen source type of table produces the influence of jasmonic to bacterial strain
| Organic nitrogen type | Production concentration (mg/L) |
| Beef extract | 849 |
| Peptone | 475 |
| Corn pulp | 850 |
| Yeast extract | 702 |
| Malt extract | 294 |
Influence of 8 macroreticular resin of embodiment to jasmonic is produced to bacterial strain
As described in Example 2, when cultivating after inoculation the 3-5 days, be added into culture medium sterilized XAD-1,
XAD-2, XAD-7HP, AB-8, D101,5~20% (W/V), absorbed portion product are avoided when production concentration reaches in culture solution
After certain, not being further added by can decompose instead.After fermentation, separation resin and supernatant, are eluted with ethyl acetate, are revolved with vacuum
Turn evaporimeter and ethyl acetate is evaporated off.The concentration such as table 7 of HPLC survey product.
7 macroreticular resin of table produces the influence of jasmonic to bacterial strain
| Resin type | Production concentration (mg/L) |
| XAD-1 | 1210 |
| XAD-2 | 1760 |
| XAD-7HP | 1527 |
| AB-8 | 1760 |
| D101 | 1720 |
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
SEQUENCE LISTING
<110>Southern Yangtze University
<120>a kind of method that Lasiodiplodia iranensis bacterium and its fermentation produce jasmonic
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 519
<212> DNA
<213> Lasiodiplodia iranensis
<400> 1
cctgcggaag gatcattacc gagttttcgg gcttcggctc gactctccca ccctttgtga 60
acgtacctct gttgctttgg cggctccggc cgccaaagga cctccaaact ccagtcagta 120
aacgcagacg tctgataaac aagttaataa actaaaactt tcaacaacgg atctcttggt 180
tctggcatcg atgaagaacg cagcgaaatg cgataagtaa tgtgaattgc agaattcagt 240
gaatcatcga atctttgaac gcacattgcg ccccttggta ttccgggggg catgcctgtt 300
cgagcgtcat tacaaccctc aagctctgct tggaattggg caccgtcctc actgcggacg 360
cgcctcaaag acctcggcgg tggctgttca gccctcaagc gtagtagaat acacctcgct 420
ttggagtggt tggcgtcgcc cgccggacga accttctgaa cttttctcaa ggttgacctc 480
ggatcaggta gggatacccg ctgaacttaa gcatatcaa 519
Claims (24)
1.Lasiodiplodia iranensis DWH-2 is preserved in China typical culture collection on May 27th, 2017
Center, deposit number are CCTCC NO:M2017288, and preservation address is Wuhan, China university.
2. a kind of method for producing jasmonic using the fermentation of L.iranensis DWH-2 described in claim 1, which is characterized in that
It is cultivated under the conditions of static or reciprocating concussion or rotary concussion.
3. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 2 produces jasmonic
Method, which is characterized in that be included in 20~30 DEG C, static fermented and cultured 6~10 days.
4. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 2 or 3 produces jasmine
The method of acid, which is characterized in that the carbon source of fermentation medium includes one of sucrose, starch, maltose, glucose, fructose
Or several mixing, carbon source concentration are 25~500g/L.
5. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 2 produces jasmonic
Method, which is characterized in that inorganic nitrogen-sourced the mixing including one or more of sodium nitrate, urea, ammonium nitrate of fermentation medium
It closes, inorganic nitrogen-sourced concentration is 3~15g/L.
6. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 3 produces jasmonic
Method, which is characterized in that inorganic nitrogen-sourced the mixing including one or more of sodium nitrate, urea, ammonium nitrate of fermentation medium
It closes, inorganic nitrogen-sourced concentration is 3~15g/L.
7. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 4 produces jasmonic
Method, which is characterized in that inorganic nitrogen-sourced the mixing including one or more of sodium nitrate, urea, ammonium nitrate of fermentation medium
It closes, inorganic nitrogen-sourced concentration is 3~15g/L.
8. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 2 produces jasmonic
Method, which is characterized in that the organic nitrogen source of fermentation medium includes beef extract, peptone, corn pulp, yeast extract, malt extraction
The mixing of one or more of object, organic nitrogen source concentration are 3~15g/L.
9. a kind of ferment using L.iranensis DWH-2 described in claim 1 according to claim 3 produces jasmonic
Method, which is characterized in that the organic nitrogen source of fermentation medium includes beef extract, peptone, corn pulp, yeast extract, malt extraction
The mixing of one or more of object, organic nitrogen source concentration are 3~15g/L.
10. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 4 produces jasmonic
Method, which is characterized in that the organic nitrogen source of fermentation medium includes that beef extract, peptone, corn pulp, yeast extract, malt mention
The mixing of one or more of object is taken, organic nitrogen source concentration is 3~15g/L.
11. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 5 produces jasmonic
Method, which is characterized in that the organic nitrogen source of fermentation medium includes that beef extract, peptone, corn pulp, yeast extract, malt mention
The mixing of one or more of object is taken, organic nitrogen source concentration is 3~15g/L.
12. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 2 produces jasmonic
Method, which is characterized in that fermentation medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, potassium dihydrogen phosphate 0.5~
3g/L, 0.1~0.4g/L of potassium chloride, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, 1~5g/L of corn pulp,
0.2-20g/L soybean oil, 10~12ml/L of microelement, pH4.5~8；The microelement: green-vitriol 0.01~
0.04g/L, seven water manganese sulfate 0.001~0.004g/L, seven 0.001~0.004g/L of brochanite, two molybdic acid hydrate sodium 0.001
~0.004g/L.
13. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 3 produces jasmonic
Method, which is characterized in that fermentation medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, potassium dihydrogen phosphate 0.5~
3g/L, 0.1~0.4g/L of potassium chloride, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, 1~5g/L of corn pulp,
0.2-20g/L soybean oil, 10~12ml/L of microelement, pH4.5~8；The microelement: green-vitriol 0.01~
0.04g/L, seven water manganese sulfate 0.001~0.004g/L, seven 0.001~0.004g/L of brochanite, two molybdic acid hydrate sodium 0.001
~0.004g/L.
14. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 4 produces jasmonic
Method, which is characterized in that fermentation medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, potassium dihydrogen phosphate 0.5~
3g/L, 0.1~0.4g/L of potassium chloride, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, 1~5g/L of corn pulp,
0.2-20g/L soybean oil, 10~12ml/L of microelement, pH4.5~8；The microelement: green-vitriol 0.01~
0.04g/L, seven water manganese sulfate 0.001~0.004g/L, seven 0.001~0.004g/L of brochanite, two molybdic acid hydrate sodium 0.001
~0.004g/L.
15. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 5 produces jasmonic
Method, which is characterized in that fermentation medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, potassium dihydrogen phosphate 0.5~
3g/L, 0.1~0.4g/L of potassium chloride, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, 1~5g/L of corn pulp,
0.2-20g/L soybean oil, 10~12ml/L of microelement, pH4.5~8；The microelement: green-vitriol 0.01~
0.04g/L, seven water manganese sulfate 0.001~0.004g/L, seven 0.001~0.004g/L of brochanite, two molybdic acid hydrate sodium 0.001
~0.004g/L.
16. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 6 produces jasmonic
Method, which is characterized in that fermentation medium is 25~500g/L of sucrose, 1~15g/L of sodium nitrate, potassium dihydrogen phosphate 0.5~
3g/L, 0.1~0.4g/L of potassium chloride, 1~9g/L of epsom salt, 0.1~0.9g/L of green-vitriol, 1~5g/L of corn pulp,
0.2-20g/L soybean oil, 10~12ml/L of microelement, pH4.5~8；The microelement: green-vitriol 0.01~
0.04g/L, seven water manganese sulfate 0.001~0.004g/L, seven 0.001~0.004g/L of brochanite, two molybdic acid hydrate sodium 0.001
~0.004g/L.
17. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 2 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
18. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 3 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
19. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 4 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
20. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 5 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
21. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 6 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
22. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 7 produces jasmonic
Method, which is characterized in that during the fermentation, macroreticular resin is added into fermentation system.
23. the fermentation of L.iranensis DWH-2 described in a kind of application claim 1 according to claim 17 produces jasmonic
Method, which is characterized in that the model of macroreticular resin includes XAD-1, XAD-2, XAD-7HP, AB-8 or D101, dosage is 5~
20g/100mL。
24. L.iranensis DWH-2 described in claim 1 promotees in production fragrance, plant drought performance reinforcing agent, plant blossom
Application into agent, insect pest preparation.
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| CN112481140B (en) * | 2020-12-23 | 2022-03-15 | 江南大学 | Method for producing jasmonic acid by microbial solid state fermentation |
| CN112812974A (en) * | 2021-01-15 | 2021-05-18 | 赵万洲 | Production process for separating and obtaining cocoa trichite for efficiently producing jasmonic acid from tropical fruits |
| CN112626140A (en) * | 2021-01-18 | 2021-04-09 | 赵万洲 | Efficient and rapid production process for biologically synthesizing jasmonic acid |
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