CN108244638A - Kalimeris fermentate and its preparation method and application - Google Patents
Kalimeris fermentate and its preparation method and application Download PDFInfo
- Publication number
- CN108244638A CN108244638A CN201810086362.5A CN201810086362A CN108244638A CN 108244638 A CN108244638 A CN 108244638A CN 201810086362 A CN201810086362 A CN 201810086362A CN 108244638 A CN108244638 A CN 108244638A
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- China
- Prior art keywords
- kalimeris
- fermentate
- fermentation
- preparation
- temperature
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention discloses a kind of kalimeris fermentates and its preparation method and application.The fermentation of different condition three times is carried out using the culture medium of the zymolyte containing kalimeris, gained fermentate progress separation of solid and liquid is then obtained into fermenation raw liquid, as kalimeris fermentate.The preparation method fermentation time of the present invention is short, so as to be more conducive to industrialized production.In addition, the fermentation method of the present invention can also improve the active constituent in gained fermentate, drug effect, particularly anti-gastric-ulcer effect are significantly improved.
Description
Technical field
The present invention relates to a kind of kalimeris fermentates and its preparation method and application, especially a kind of to have anti-ulcer effect
Kalimeris fermentate and its preparation method and application.
Background technology
Kalimeris is the traditional herbal medicines that a kind of resource is wide, has certain anti-ulcer effect, but opening for kalimeris at present
The mainly traditional water extraction method of hair.This method technique falls behind, and active principle dissolution is low, and drug effect maximum cannot play, this
Limit the exploitation of kalimeris Related product.
Herb fermenting puies forward technology than traditional water great progress, is converted including traditional Chinese medicine ingredients by zymophyte, by macromolecular
Become small molecule, active principle is more prone to be dissolved out, and taste is more prone to receive.
The research fermented currently for kalimeris is simultaneously few.For example, CN107260812A disclose a kind of ferment and its
Preparation process is made of following raw material：0.5 part of wild mint, 0.4 part of Herba portulacae, 0.4 part of Asiatic plantain, 0.4 part of dandelion are grey
0.5 part of the ears or side handles of a utensil, 0.4 part of kalimeris, 0.4 part of herba setariae viridis grass, 2 parts of glucose, 15 parts of water.Ferment disclosed in the document has heat-clearing solution
Poison, the antiviral effect of eczema diuresis.
Therefore, kalimeris resource how is further developed and used, it is a subject for being worth research to improve kalimeris utilization rate.
Invention content
The purpose of the present invention is to provide the preparation method of a kind of kalimeris fermentate or kalimeris fermentation dry powder, this method can be with
It ferments using only the single raw material of kalimeris, fermentation time is short, and gained fermentate drug effect greatly promotes.The present invention's is another
A kind of kalimeris fermented product is designed to provide, there is improved anti-ulcer effect.Another object of the present invention is above-mentioned
The purposes of kalimeris fermented product.The purpose of the present invention is what is be achieved through the following technical solutions.
On the one hand, the present invention provides a kind of preparation method of kalimeris fermentate, includes the following steps：
First fermentation step：8~18h of anaerobic fermentation is carried out in the medium at 26~32 DEG C using saccharomycete, is obtained
First fermentate, wherein the culture medium includes kalimeris zymolyte；
Second fermentation step：The temperature of first fermentate is risen to 33~40 DEG C, and at such a temperature using lactic acid bacteria into
Row 30~40h of standing for fermentation, obtains the second fermentate,
Third fermentation step：The temperature of second fermentate is risen to 50~70 DEG C, and at such a temperature standing for fermentation 20~
30h obtains third fermentate；
Solid-liquid separation step：Gained third fermentate progress separation of solid and liquid is obtained into fermenation raw liquid, as kalimeris fermentate.
Preparation method according to the present invention, it is preferable that the kalimeris zymolyte is by using trypsase, cellulose
Enzyme and zytase digest water slurry 2~6h systems of the powder containing kalimeris at a temperature of 5.5~6.8 pH value and 45~60 DEG C
.
Preparation method according to the present invention, it is preferable that the dosage of the trypsase is water slurry total weight
0.05~0.3wt%, the dosage of the cellulase is 0.05~0.3wt% of water slurry total weight and the xylan
The dosage of enzyme is 0.05~0.3wt% of water slurry total weight.
Preparation method according to the present invention, it is preferable that the amount ratio of trypsase, cellulase and zytase is
1:1:1。
Preparation method according to the present invention, it is preferable that kalimeris powder and water in the water slurry of the powder containing kalimeris
Weight ratio is 1:5~15.
Preparation method according to the present invention, it is preferable that the culture medium further includes 0.1~0.5wt% yeast
Cream, 1~5wt% ammonium salts, 0.05~0.2wt% magnesium salts and 0.3~0.8wt% phosphate；More than weight percent is all based on
The total weight of the culture medium.
Preparation method according to the present invention, it is preferable that the inoculum concentration of saccharomycete described in the first fermentation step is institute
State 0.1~1.0wt% of culture medium, the inoculum concentration of lactic acid bacteria described in the second fermentation step for the culture medium 0.5~
1.0wt%, and the inoculum concentration of the lactic acid bacteria is more than the inoculum concentration of saccharomycete.
On the other hand, the present invention provides a kind of preparation method of fermentation dry powder, includes the following steps：Using any of the above-described
Preparation method obtains kalimeris fermentate, the kalimeris fermentate then is dried obtained fermentation dry powder, and the fermentation is dry
The content of general flavone is more than 1.8wt% in powder.
Another aspect, the present invention provide a kind of kalimeris fermented product, are obtained for any one preparation method as described above
Kalimeris fermentate or fermentation dry powder.
In another aspect, the present invention, which provides above-mentioned kalimeris fermented product, is preparing drug, food with anti-ulcer effect
Or the purposes in feed addictive.
The present invention combines the mode fermented three times by using enzymolysis so that fermentation time is reduced, so as to be more conducive to
Industrialized production.In addition, the fermentation method of the present invention can also improve the active constituent in gained fermentate, drug effect is significantly improved,
Particularly anti-gastric-ulcer effect.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
Kalimeris is composite family kalimeris platymiscium Kalimeris indica (L.) Sch.-Bip also known as Herba Kalimeridis, loach string, chicken
Youngster's intestines, Tian Bianju, roadside chrysanthemum, kangaroo-paw, spleen grass.Kalimeris contains there are many drug ingedient, including flavonoids, volatile oil and vitamin
Class substance etc..In addition, drug efficacy study also indicates that kalimeris to digestive system, cardiovascular system plays a role clearly, while also has
There is certain bactericidal effect.Toxicologic study also indicates that the toxicity of kalimeris is low, and chronic administration is also without cumulative appearance.
The application is low for existing kalimeris processing method effective component extraction efficiency, proposes to combine based on enzymolysis kalimeris Chinese medicine
The scheme that fermentation is handled three times, and the present invention is completed based on this method.Specifically, the present invention includes the following contents.
The first aspect of the present invention provides a kind of preparation method of kalimeris fermentate, including fermentation step three times and admittedly
Liquid separating step.The following detailed description of each step.
<First fermentation step>
First fermentation step is saccharomycetes to make fermentation step, is carried out in the medium at 26~32 DEG C including the use of saccharomycete
8~18h of anaerobic fermentation obtains the first fermentate, wherein the culture medium includes kalimeris zymolyte.
It ferments to generate to kalimeris under anaerobic using saccharomycete and has facilitation to intestinal beneficial bacterium proliferation
Effective metabolite.0.1~1.0wt% of the inoculum concentration of saccharomycete for culture medium in first fermentation, preferably 0.2~
0.8wt%, more preferably 0.4~0.6wt%.Any strain known in the art can be used in saccharomycete, especially not specific.Example
Such as, commercially available saccharomycete can be used.
The fermentation temperature of first fermentation is 26~32 DEG C, preferably 28~31 DEG C, more preferably 30 DEG C, this temperature range one
Aspect is conducive to the fermentation of saccharomycete, and also helps the biological conversion of kalimeris active ingredient.If the first fermentation temperature
It is excessively high, then it can influence the activity of saccharomycete.
The fermentation time of first fermentation is 8~18h, more preferably preferably 10~15h, 12h.This fermentation time can make
The effect for obtaining saccharomycete gives full play to and saves the time.
Culture medium in first fermentation step need to include kalimeris zymolyte.Kalimeris zymolyte is makes to be conducive to by enzymatic treatment
The ingredient release of fermentation or the enzymatic treatment object being unfavorable for after the ingredient breakdown of fermentation.Preferably, kalimeris zymolyte be by using
The mixture obtained after trypsase, cellulase and zytase three's combined treatment kalimeris.Three kinds of enzymes can be in the application institute
Efficiently effect simultaneously, promotes enzymolysis, and there is no the adverse effects between enzymatic activity under the conditions of stating.
The condition of enzymatic treatment (being also referred to as sometimes herein " enzymolysis ") is not particularly limited, for example, can 5.5~6.8 pH
Value and 45~60 DEG C at a temperature of digest the powder containing kalimeris water slurry 2~6h.Preferably, the pH value of enzymatic treatment for 5.6~
6.5, more preferable 6, this pH value range can ensure that three kinds of enzymes described herein keep high activity simultaneously.Preferably, enzymatic treatment
Temperature is 50~60 DEG C, more preferable 52~58 DEG C, further preferred 54~56 DEG C.It is preferable to use be resistant to higher temperatures in the application
The enzyme of degree, because too low hydrolysis temperature is unfavorable for the release of anti-ulcer effect ingredient.If hydrolysis temperature is excessively high, on the one hand
Cost increases, and on the other hand can destroy the structure of part chromocor compound in kalimeris.Enzyme processing time is preferably 3~4 hours, example
Such as 3.5 hours.
The dosage of trypsase, cellulase and zytase during enzymatic treatment, the total weight based on suspension may respectively be
0.05~0.3wt%.It is preferred that being respectively 0.06~0.2wt%, more preferably respectively 0.08~0.15wt% is further preferably respectively
0.1~0.12wt%.The fermented and cultured that is combined as of three kinds of enzymes provides the raw materials such as amino acid, monosaccharide, oligosaccharides.The amount ratio of three kinds of enzymes
It is especially not specific, for example, the amount ratio of trypsase, cellulase and zytase can be 1:1:1.
It can be herb or root for the kalimeris of enzymatic treatment, this is not particularly limited.Preferably, for the horse of enzymatic treatment
Orchid is powder, and the fineness of powder is not particularly limited, preferably thinner powder, so as to be more advantageous to the progress of enzymolysis.Preferably, will
Kalimeris powder is mixed with water slurry with water, uses it for enzymatic treatment.Wherein the amount ratio of kalimeris powder and water does not limit especially
It is fixed, preferably 1:5~15, for example, 1:10, the amount ratio is based on weight.Pure water, deionized water and distilled water can be used in water.
The pH of water is preferably 6.0~6.5, and the water in the range of this pH is more advantageous to the progress of enzymolysis.
Culture medium in first fermentation step also may include nutriment.Preferably, nutriment may be selected from yeast extract, ammonium
At least one of salt, magnesium salts and phosphate ingredient.Total weight based on culture medium, culture medium may include 0.1~0.5wt% ferment
Female cream, 1~5wt% ammonium salts, 0.05~0.2wt% magnesium salts and 0.3~0.8wt% phosphate.Commercially available yeast extract may be used.
The dosage of yeast extract is preferably 0.2~0.3wt%.Ammonium salt can be selected from least one of ammonium sulfate, ammonium nitrate or ammonium carbonate,
Preferably ammonium sulfate.The dosage of ammonium salt is preferably 1.5~3.5wt%, more preferably 2~2.5wt%.Magnesium salts can be selected from sulfuric acid
At least one of magnesium, magnesium chloride, magnesium nitrate, magnesium carbonate, preferably magnesium sulfate.The dosage of magnesium salts is preferably 0.06~
0.15wt%, more preferably 0.08~0.1wt%.Phosphate can be selected from sodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, phosphorus
At least one of sour potassium, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium phosphate, magnesium phosphate, preferably potassium dihydrogen phosphate.Phosphate
Dosage be preferably 0.35~0.65wt%, more preferably 0.5~0.6wt%.Nutriment and ratio in above range, more
Be conducive to meet saccharomycete, lactic acid bacteria fermented and cultured nutrient demand, and fermentation time can be reduced.
The nutriment of the present invention can also contain sylvite.The sylvite and phosphate are preferably same substance, such as phosphoric acid
Potassium, dipotassium hydrogen phosphate or potassium dihydrogen phosphate, preferably potassium dihydrogen phosphate.
<Second fermentation step>
The step of second fermentation step continues fermentation for addition lactic acid bacteria, including the temperature of the first fermentate is risen to 33~
40 DEG C, and 30~40h of standing for fermentation is carried out using lactic acid bacteria at such a temperature, obtain the second fermentate.
The inoculum concentration of lactic acid bacteria for culture medium 0.5~1.0wt%, preferably 0.5~0.8wt%, more preferably 0.5~
0.6wt%.The inoculum concentration of saccharomycete when the inoculum concentration of lactic acid bacteria need to be more than the first fermentation.If the inoculum concentration of lactic acid bacteria is too low,
Then the amount of saccharomycete is relatively excessive during the first fermentation, influences the proliferation of lactic acid bacteria, and then influence ferment effect.
The temperature of second fermentation step need to be higher than the temperature of the first fermentation step, more excellent preferably in the range of 33~40 DEG C
Select 35~39 DEG C, even more preferably 37 DEG C.Such temperature simulates work of the lactic acid bacteria in human body close to human body environment's temperature
With temperature, so as to which the active constituent for being converted into or being discharged from Chinese medicine is more advantageous to absorption of human body.
The time of second fermentation is not particularly limited, and is under normal conditions 30~40h, preferably 35~39h, more preferably
36h.Fermentation time is long, and the rate of bioconversion can become to decline, while can generate other by-products, these by-products are unfavorable
In the performance of drug effect.On the other hand, if fermentation time is too short, react insufficient, some drugs active ingredient not yet generates,
So as to which the drug effect for making gained fermentate reduces, and can cause the wasting of resources.
Second fermentation needs to be left to ferment.Standing for fermentation is more closely similar to the environment of internal beneficial flora, thus is preferred.
<Third fermentation step>
The step of third fermentation step is saccharomycete and lactic acid bacteria double bacterium hot fermentations, including by the temperature of the second fermentate
50~70 DEG C are risen to, and is left to ferment 20~30h at such a temperature, obtains third fermentate.
By the way that saccharomycete and lactic acid bacteria is made to be carried out at the same time and continue to ferment at 50~70 DEG C, can more effectively promote effectively into
Such as flavones content is divided quickly to generate in a short time.Preferably 55~65 DEG C, more preferable 60 DEG C of the range of second fermentation temperature.
At this temperature, only it is left to ferment 20~30h, preferably 21~28h, more preferable 24~26h can obtain having the of required performance
Three fermentates substantially reduce the fermentation time in conventional method.
<Solid-liquid separation step>
Produced object solid residue when separation of solid and liquid is for removing fermentation, and recycle fermenation raw liquid.Separation of solid and liquid can be used
Any known method, including but not limited to centrifuge, filter, precipitating etc..Preferably, centrifugation mode can be used in separation of solid and liquid,
For example, it is detached using centrifuge.The actual conditions of centrifugation are not particularly limited, and can be adjusted as needed and suitably.Pass through
Isolated fermenation raw liquid, as kalimeris fermentate.
The second aspect of the present invention provides a kind of preparation method of fermentation dry powder, and kalimeris is obtained using above-mentioned preparation method
Then obtained fermentation dry powder is dried in the kalimeris fermentate by fermentate, and in the fermentation dry powder general flavone content
It is more than 1.8%.For example, above-mentioned kalimeris fermentate is obtained into kalimeris fermentation dry powder by dry and/or concentration step.It is dry
And/or concentration step can be used for removing influence of the medium molecular chaperones for drug effect.Preferably, before the drying step
Including concentration step.Yeast powder is can obtain by concentrating and drying.The method that decompression may be used is concentrated and is dried, here
It repeats no more.
The third aspect of the present invention provides a kind of kalimeris fermented product, to pass through preparation method system of the present invention
Standby obtained kalimeris fermentate or kalimeris fermentation dry powder.According to embodiment of the present invention, the fermentation dry powder is aqueous
Amount is more than or equal to 420 ± 5U/g less than 5wt%, the vigor of superoxide dismutase SOD, and viable count of lactobacillus is more than or equal to 3.3
±0.5×108CFU/g.The content of the general flavone of fermentation dry powder is more than 1.8wt%.
The fourth aspect of the present invention, provide kalimeris fermented product prepare with the drug of anti-ulcer effect, food or
Purposes in feed addictive.Wherein additive amount of the kalimeris fermentate in drug, food or feed addictive is not particularly limited.
Example as drug includes but not limited to pulvis, pill solid dosage forms, further includes suspending agent, intermixture, emulsification
The liquid dosage forms such as agent.Example as food includes but not limited to candy, beverage, bread, biscuit etc..Feed addictive refers to
The a small amount of or micro substance added during Feed Manufacturing processing, use, dosage is seldom in feed but effect is notable.
1 kalimeris fermentate of embodiment and beverage
1) it crushes：Kalimeris be crushed into the sieve of pharmacopeia 2, then add in 10 times of raw material weights than pure water, be made and suspend
Liquid.
2) preparation of enzymolysis liquid：Weight based on suspension, addition trypsase 0.1wt% (500,000 U/g), cellulase
0.1wt% (500,000 U/g), zytase 0.1wt% (500,000 U/g) digest suspension.The pH controls of suspension during enzymolysis
6.0 are made as, temperature is 55 DEG C, enzymolysis time 3h.
3) nutriment is added：Total weight based on enzymolysis liquid adds yeast extract 0.2wt%, ammonium sulfate into enzymolysis liquid
2wt%, magnesium sulfate 0.1wt%, potassium dihydrogen phosphate 0.6wt%, fermentation substrate sterilizing.
4) it is inoculated with and ferments：It is inoculated with 0.5wt% saccharomycete, the anaerobic fermentation 12h under the conditions of 30 DEG C.Then, it is inoculated with
0.6wt% lactic acid bacteria fermenting agents are left to ferment 36h under the conditions of 37 DEG C.It is 60 DEG C finally to maintain temperature, stands for 24 hours, is sent out
Ferment.
5) it centrifuges：Centrifugal treating, kalimeris fermentate of the isolated fermenation raw liquid as the present invention are carried out after fermentation.
6) it is concentrated and dried：Fermenation raw liquid after separation is concentrated under reduced pressure, fermentation dry powder is obtained by being dried under reduced pressure.
7) Beverage Service：And by fermenation raw liquid and water 1:10 weight ratio adds in, and adds 18wt% sucrose, 0.3wt%
Citric acid, and-filling-cooling that carries out homogeneous-degassing-pasteurize and etc., obtain finished beverage.
Embodiment 2- kalimeris fermentate and dry powder
1) it crushes：Kalimeris be crushed into the sieve of pharmacopeia 2, then add in 10 times of raw material weights than pure water, be made and suspend
Liquid.
2) preparation of enzymolysis liquid：Weight based on suspension, addition trypsase 0.1wt% (500,000 U/g), cellulase
0.1wt% (500,000 U/g), zytase 0.1wt% (500,000 U/g) digest suspension.The pH controls of suspension during enzymolysis
6.2 are made as, temperature is 56 DEG C, enzymolysis time 3h.
3) nutriment is added：Total weight based on enzymolysis liquid adds yeast extract 0.2wt%, ammonium sulfate into enzymolysis liquid
2wt%, magnesium sulfate 0.1wt%, potassium dihydrogen phosphate 0.6wt%, fermentation substrate sterilizing.
4) it is inoculated with and ferments：0.5wt% saccharomycete is inoculated with, anaerobic fermentation 12h under the conditions of 29 DEG C.Then, it is inoculated with 0.6wt%
Lactic acid bacteria fermenting agent is left to ferment 36h under the conditions of 37 DEG C.It is 60 DEG C finally to maintain temperature, stands for 24 hours, ferments.
5) it centrifuges：Centrifugal treating, kalimeris fermentate of the isolated fermenation raw liquid as the present invention are carried out after fermentation.
6) it concentrates：Fermenation raw liquid after separation is concentrated under reduced pressure, by being dried under reduced pressure to obtain fermentation dry powder.
The determination experiment of active ingredient general flavone content in embodiment 3- kalimeris fermentates
1. prepared by sample liquid
Kalimeris fermentate sample：Fermentation dry powder 2g made from Example 2 (is equivalent to kalimeris 35g crude drug in whole), adds in and steams
Distilled water be settled to 100ml to get.
Kalimeris Aqueous extracts sample：Kalimeris 35g is taken, water impregnates 30min, decocts extraction 2 times, and each 10 times of amount of water is measured, often
Secondary 2 hours extraction times, filtering, filtrate merge, rotation rotary evaporation concentration, with distilled water be settled to 100ml to get.
2. the preparation of reference substance solution
Precision weighs the control substance of Rutin 10mg through 120 DEG C of freeze-day with constant temperature to constant weight, puts in the measuring bottle of 50mL, adds appropriate second
Alcohol, makes it all dissolve, lets cool with supersound process, and ethanol in proper amount is then added to be shaken up to graduation mark up to required control again
Product solution (contains anhydrous rutin 0.2mg) in per 1mL.
3. reference substance absorbance measurement
Precision measures reference substance solution 6mL, is respectively placed in the measuring bottle of 50mL, then adds water to 12mL respectively, drips
Add 5wt% sodium nitrite solution 2mL, shake up, place 6min, 10wt% aluminum nitrate solution 2mL are then added dropwise respectively again, shake up,
6min is placed, then 4% sodium hydroxide test solution 20mL is added dropwise, finally adds water to graduation mark, shakes up, places 15min, with mutually taking an entrance examination
Agent is as blank control group, immediately after according to UV-VIS spectrophotometry, set wavelength measured at 500nm absorbance as
0.251。
4. assay
Sampling liquid 10mL is respectively placed in the measuring bottle of 50mL, then adds water to 12mL respectively, then 5wt% nitrous is added dropwise respectively
Acid sodium solution 2mL, shakes up, and places 6min, and 10wt% aluminum nitrate solution 2mL are then added dropwise respectively again, shake up, and places 6min, then
4% sodium hydroxide test solution 20mL is added dropwise, finally adds water to graduation mark, shakes up, places 15min, by the use of corresponding reagent as blank
Control group, immediately after according to UV-VIS spectrophotometry, setting wavelength measures the extinction of each group sample solution at 500nm
It spends (3 parts of parallel sample), record experiment gained each group absorbance.It is calculated according to formula C=5*A*0.024/0.251 total in sample liquid
Flavones content (unit mg/mL).
General flavone content in two kinds of sample liquids is calculated according to above-mentioned curve.
The measurement result of 1 kalimeris fermentate sample of table and kalimeris Aqueous extracts sample general flavone content
Embodiment 4- effect experiments
1. the preparation of drug：
Fermentation dry powder 2g made from Example 2 (is equivalent to kalimeris 35g crude drug in whole), adds in distilled water and is settled to 100ml,
To obtain the final product.(calculating volume according to intragastric administration on mice volume 20ml/kg)
It is prepared by Aqueous extracts：Kalimeris 35g is taken, water impregnates 30min, decocts extraction 2 times, and each 10 times of amounts of amount of water carry every time
It takes time 2 h, filters, filtrate merges, and rotation rotary evaporation concentration is settled to 100ml with distilled water to obtain the final product.
2. experimental method：
Healthy Kunming mouse 30 is taken, half male and half female is randomly divided into 3 groups：Blank control group, kalimeris fermentate group, horse
Blue Aqueous extracts group.The daily gastric infusion of each group is primary, continuous 5 days.After last dose 1h stress, be deprived of food but not water before experiment
12h.During experiment, by mouse bondage on iron railings, being put into 20 DEG C or so of water-bath, liquid level is maintained at ensiform process of sternum level.24h
After put to death mouse, take stomach, ligature stomach cardia and pylorus and 1% formalin 2mL is injected into gastral cavity, stomach is immersed into formalin
In, it is splitted after 30min along greater curvature, washes away gastric content, observation gastric mucosa damage and calculated gastric ulcer under Stereo microscope and refer to
Number.
Ulcer index computational methods：Ulcer point or below face 1mm persons count 1 point；1-2mm person counts 2 points；2-3mm person, meter
3 points；3-4mm person counts 4 points；More than 4mm person, 5 points are counted, score is added as to the ulcer index of this animal, and is calculated routed
Ulcer inhibiting rate.Ulcer inhibition rate (%)=(solvent control group ulcer index-administration group ulcer index)/solvent control group ulcer
Index × 100%.The results are shown in Table 2.
2 kalimeris fermentate of table is with regard to influence of the kalimeris decocting liquid to chmice acute stress gastric ulcer model
| Group | Mouse/only | Dosage/(g/kg) | Ulcer index |
| Blank control group | 10 | - | 45.70±6.00△△ |
| Kalimeris fermentate group | 10 | 7 | 28.40±4.38** |
| Kalimeris Aqueous extracts group | 10 | 7 | 37.40±6.80**△△ |
Compared with blank group, 0.05 * * P ＜ 0.01 of * P ＜；
Compared with kalimeris fermentate group, △ P ＜ 0.05, △ △ P ＜ 0.01.
By experimental data statistical result it is found that each administration group reduces (P ＜ with blank group ulcer index pole conspicuousness
0.01)；Kalimeris fermentate group substantially reduces (P ＜ 0.01) compared with Aqueous extracts group ulcer index pole.The experiment results show that kalimeris water carries
Liquid and kalimeris fermentate have anti-ulcer effect, and kalimeris fermentate anti-ulcer effect is substantially better than kalimeris Aqueous extracts.
Present invention is not limited to the embodiments described above, in the case of without departing substantially from the substantive content of the present invention, this field skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of preparation method of kalimeris fermentate, which is characterized in that include the following steps：
First fermentation step：8~18h of anaerobic fermentation is carried out in the medium at 26~32 DEG C using saccharomycete, obtains first
Fermentate, wherein the culture medium includes kalimeris zymolyte；
Second fermentation step：The temperature of first fermentate is risen to 33~40 DEG C, and quiet using lactic acid bacteria progress at such a temperature
30~40h of fermentation is put, obtains the second fermentate,
Third fermentation step：The temperature of second fermentate is risen to 50~70 DEG C, and be left to ferment 20~30h at such a temperature,
Obtain third fermentate；
Solid-liquid separation step：Gained third fermentate progress separation of solid and liquid is obtained into fermenation raw liquid, as kalimeris fermentate.
2. preparation method according to claim 1, which is characterized in that the kalimeris zymolyte by using trypsase,
Cellulase and zytase digested at a temperature of 5.5~6.8 pH value and 45~60 DEG C the water slurry 2 of the powder containing kalimeris~
6h is made.
3. preparation method according to claim 2, which is characterized in that the dosage of the trypsase is water slurry gross weight
0.05~0.3wt% of amount, the dosage of the cellulase is 0.05~0.3wt% of water slurry total weight and the wood
The dosage of dextranase is 0.05~0.3wt% of water slurry total weight.
4. preparation method according to claim 3, which is characterized in that the use of trypsase, cellulase and zytase
Amount is than being 1:1:1.
5. preparation method according to claim 1, which is characterized in that in the water slurry of the powder containing kalimeris, kalimeris
The weight ratio of powder and water is 1:5~15.
6. preparation method according to claim 1, which is characterized in that the culture medium further includes 0.1~0.5wt%
Yeast extract, 1~5wt% ammonium salts, 0.05~0.2wt% magnesium salts and 0.3~0.8wt% phosphate；More than weight percent is
Total weight based on the culture medium.
7. preparation method according to claim 1, which is characterized in that the inoculum concentration of saccharomycete described in the first fermentation step
For 0.1~1.0wt% of the culture medium, the inoculum concentration of lactic acid bacteria described in the second fermentation step is the 0.5 of the culture medium
~1.0wt%, and the inoculum concentration of the lactic acid bacteria is more than the inoculum concentration of saccharomycete.
8. a kind of preparation method of fermentation dry powder, which is characterized in that include the following steps：Using any one of claim 1~7 institute
The preparation method stated obtains kalimeris fermentate, and the kalimeris fermentate then is dried obtained fermentation dry powder, and the hair
The content of general flavone is more than 1.8wt% in ferment dry powder.
9. a kind of kalimeris fermented product, which is characterized in that it is obtains according to claim 1~7 any one of them preparation method
The fermentation dry powder that the kalimeris fermentate obtained or preparation method according to claim 8 obtain.
10. kalimeris fermented product according to claim 9 is preparing drug, food or feed with anti-ulcer effect
Purposes in additive.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112956602A (en) * | 2021-03-22 | 2021-06-15 | 安徽希普生物科技有限公司 | Fish protein powder composition with effect of resisting gastrointestinal ulcer and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012184257A (en) * | 2012-06-19 | 2012-09-27 | Kracie Foods Ltd | Low-density lipoprotein antioxidant and food and drink product using the same |
| CN103830302A (en) * | 2014-02-24 | 2014-06-04 | 贵阳医学院 | Preparation method and application of effective component of herba kalimeridis |
| CN103865815A (en) * | 2014-02-25 | 2014-06-18 | 北京观澜科技有限公司 | Method for preparing biological humic acid bacteria agent from biological organic enzymolysis-coupled conversion antibiotic dregs |
| CN105924479A (en) * | 2016-06-22 | 2016-09-07 | 浙江大学 | Indian kalimeris herb active component and compounds as well as preparation and application |
| CN105962360A (en) * | 2016-05-09 | 2016-09-28 | 山东祥维斯生物科技股份有限公司 | Method for producing microorganism ferment |
| CN106617051A (en) * | 2016-11-21 | 2017-05-10 | 郑州市嘉蓓丽生物科技有限公司 | Fruit and vegetable ferment freeze-dried powder and preparation method thereof |
| CN107260812A (en) * | 2016-04-08 | 2017-10-20 | 张艳玲 | A kind of ferment and its preparation technology |
-
2018
- 2018-01-30 CN CN201810086362.5A patent/CN108244638A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012184257A (en) * | 2012-06-19 | 2012-09-27 | Kracie Foods Ltd | Low-density lipoprotein antioxidant and food and drink product using the same |
| CN103830302A (en) * | 2014-02-24 | 2014-06-04 | 贵阳医学院 | Preparation method and application of effective component of herba kalimeridis |
| CN103865815A (en) * | 2014-02-25 | 2014-06-18 | 北京观澜科技有限公司 | Method for preparing biological humic acid bacteria agent from biological organic enzymolysis-coupled conversion antibiotic dregs |
| CN107260812A (en) * | 2016-04-08 | 2017-10-20 | 张艳玲 | A kind of ferment and its preparation technology |
| CN105962360A (en) * | 2016-05-09 | 2016-09-28 | 山东祥维斯生物科技股份有限公司 | Method for producing microorganism ferment |
| CN105924479A (en) * | 2016-06-22 | 2016-09-07 | 浙江大学 | Indian kalimeris herb active component and compounds as well as preparation and application |
| CN106617051A (en) * | 2016-11-21 | 2017-05-10 | 郑州市嘉蓓丽生物科技有限公司 | Fruit and vegetable ferment freeze-dried powder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 田徽等: ""马兰抗实验性胃溃疡作用有效部位的筛选"", 《时珍国医国药》 * |
| 陈寿宏编著: "《中华食材》", 30 April 2016, 合肥工业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112956602A (en) * | 2021-03-22 | 2021-06-15 | 安徽希普生物科技有限公司 | Fish protein powder composition with effect of resisting gastrointestinal ulcer and preparation method thereof |
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