CN108240932A - A kind of sputum specimen film-making colouring method - Google Patents
A kind of sputum specimen film-making colouring method Download PDFInfo
- Publication number
- CN108240932A CN108240932A CN201611229537.0A CN201611229537A CN108240932A CN 108240932 A CN108240932 A CN 108240932A CN 201611229537 A CN201611229537 A CN 201611229537A CN 108240932 A CN108240932 A CN 108240932A
- Authority
- CN
- China
- Prior art keywords
- sputum specimen
- sputum
- film
- making
- treatment fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010036790 Productive cough Diseases 0.000 title claims abstract description 36
- 208000024794 sputum Diseases 0.000 title claims abstract description 35
- 210000003802 sputum Anatomy 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000004040 coloring Methods 0.000 title claims abstract description 14
- 206010062717 Increased upper airway secretion Diseases 0.000 claims abstract description 14
- 239000012530 fluid Substances 0.000 claims abstract description 14
- 208000026435 phlegm Diseases 0.000 claims abstract description 14
- 238000012360 testing method Methods 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004043 dyeing Methods 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 8
- OJGDCBLYJGHCIH-UHFFFAOYSA-N bromhexine Chemical compound C1CCCCC1N(C)CC1=CC(Br)=CC(Br)=C1N OJGDCBLYJGHCIH-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229960003870 bromhexine Drugs 0.000 claims abstract description 5
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 235000019441 ethanol Nutrition 0.000 claims abstract description 4
- 239000004615 ingredient Substances 0.000 claims abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 4
- 239000010452 phosphate Substances 0.000 claims abstract description 4
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 238000010348 incorporation Methods 0.000 claims description 2
- 238000010186 staining Methods 0.000 abstract description 5
- 230000009514 concussion Effects 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The present invention relates to a kind of sputum specimen film-making colouring methods, acquire sputum specimen to be checked first, then mix sputum specimen with phlegm treatment fluid, and shake makes it decompose completely；It is transferred into test tube after sputum decomposes completely and carries out concussion centrifugal treating, discarded supernatant liquid, cell-preservation liquid is injected into test tube, film-making is carried out by automatic dyeing machine, the ingredient of wherein phlegm treatment fluid is：2 8wt% of phosphate, 2 3wt% of phenol, 2 3wt% of bromhexine, 20 40wt% of ethyl alcohol, 46 74wt% of distilled water.Sputum specimen film-making colouring method flow provided by the invention is relatively simple, and phlegm treatment fluid dosage is few, coordinates automatic dyeing machine that can disposably be prepared on a large scale qualified staining section and stable quality, monolithic speed is up to 2/min.
Description
Technical field
The invention belongs to sectioning cells staining technique fields, and in particular to a kind of sputum specimen film-making colouring method.
Background technology
Sputum is trachea-bronchial epithelial cell, secretion caused by alveolar.Such secretion is very few under normal circumstances, by disease
Bacterium infection etc. is under pathologic conditions, and amount of expectoration increases, and have the change of character, composition during such as lung inflammation, pulmonary tuberculosis, tumour.Sputum
Sample is that clinical microbiology examines most common sample, acquires sputum sample and film-making dyeing observation analysis pair is carried out to it
It is had a very important significance in the diagnosis of disease.
The pathogeny detection method of sputum includes Sputum smears method and Sputum culturing method, the former is using extensively all over the world
Method, has many advantages, such as fast and convenient inexpensive, but positive rate is relatively low and is easily influenced by sample making apparatus and reagent；The latter
Sensitivity specificity accuracy rate is higher, and as a result reliably, but cultivation cycle is longer, and efficiency is less than smear method.It strictly regulates
Sputum specimen film-making colouring method, along with good reagent and pelleter can batch making go out the microscopy piece of high quality, obtain
Reliable testing result.
Invention content
The purpose of the present invention is to provide a kind of sputum specimen film-making colouring methods, are coordinated by the phlegm treatment fluid of independent development
Corresponding sample processing method with reference to the automatic staining pelleter that our company develops efficiently can be prepared quickly and meet mirror in batches
Examine the staining section of requirement.To achieve the above object, technical scheme is as follows.
A kind of sputum specimen film-making colouring method, includes the following steps：（1）Acquire sputum specimen to be checked；（2）By sputum specimen and phlegm
Treatment fluid mixes, and shake makes its decomposition；（3）It is transferred into test tube after sputum decomposes completely and carries out concussion centrifugal treating,
Liquid is discarded supernatant, cell-preservation liquid is injected into test tube, film-making is carried out by automatic dyeing machine.
In said program, ratio when sputum specimen and phlegm treatment fluid mix is 1g：3-5mL, mixing temperature are 18-24 DEG C,
Shake incorporation time is 5-15min.
In said program, the ingredient of the phlegm treatment fluid is：Phosphate 2-8wt%, phenol 2-3wt%, bromhexine 2-3wt%,
Ethyl alcohol 20-40wt%, distilled water 46-74wt%.
In said program, the rotating speed of the centrifugal treating turns/min, centrifugation time 2-4min for 1000-1200.
Compared with prior art, sputum specimen film-making colouring method provided by the invention has the advantages that：Flow compared with
To be simple, phlegm treatment fluid dosage is few, coordinates dedicated automatic dyeing machine that can disposably be prepared on a large scale qualified staining section
And stable quality, monolithic speed reach 22*16mm up to 2/min, diagosis area.
Specific embodiment
For those of ordinary skill in the art is made to fully understand technical scheme of the present invention and advantageous effect, below in conjunction with specific
Embodiment is further described.
Embodiment 1.
A kind of sputum specimen film-making colouring method, includes the following steps：Sputum specimen to be checked is acquired first, and the acquisition of sample is tight
Lattice abide by hygienic practice, and subsequent step may all be influenced by adopting more or adopting less.Sputum specimen is transferred in plastic test tube, according to 1g：3-
The ratio of 5mL adds in phlegm treatment fluid thereto, and test tube is placed in 18-24 DEG C of water-bath, and shake makes its decomposition.When usually handling
Between for 5-15min, if sputum specimen is relatively glutinous, can suitably increasing the dosage of phlegm treatment fluid, to ensure that it is decomposed complete.（3）Treat that sputum decomposes
It is transferred into centrifuge after completely and carries out concussion gradient centrifugation processing, centrifuge speed turns/min, centrifugation for 1000-1200
Time is very short, 2-4min.Liquid is discarded supernatant after the completion of centrifugation, cell-preservation liquid is injected into test tube.It it is prepared by batch
Test tube is put into automatic dyeing machine, most 120 primary.It opens automatic dyeing machine and starts film-making, after the completion of film-making
Remove diagosis.
The ingredient of phlegm treatment fluid is：Phosphate 2-8wt%, phenol 2-3wt%, bromhexine 2-3wt%, ethyl alcohol 20-40wt% steam
Distilled water 46-74wt%.The content of wherein distintegrant bromhexine can be suitably adjusted according to actual conditions.
It should be noted that the foregoing is merely the preferred embodiment of the present invention, while in accordance with previous embodiment to this hair
It is bright to be described in detail, for those skilled in the art, can also modify on the basis of the present invention or
Person carries out equivalent replacement to some technical characteristics therein.All essence without prejudice to the present invention, any modification carried out, etc.
With replacement, improvement etc., should all include within protection scope of the present invention.
Claims (4)
1. a kind of sputum specimen film-making colouring method, which is characterized in that include the following steps：（1）Acquire sputum specimen to be checked；（2）It will
Sputum specimen is mixed with phlegm treatment fluid, and shake makes its decomposition；（3）It is transferred into test tube and is shaken after sputum decomposes completely
Centrifugal treating discards supernatant liquid, and cell-preservation liquid is injected into test tube, and film-making is carried out by automatic dyeing machine.
2. a kind of sputum specimen film-making colouring method as described in claim 1, it is characterised in that：Sputum specimen is mixed with phlegm treatment fluid
When ratio be 1g：3-5mL, mixing temperature are 18-24 DEG C, and shake incorporation time is 5-15min.
A kind of 3. sputum specimen film-making colouring method as described in claim 1, which is characterized in that the ingredient of the phlegm treatment fluid
For：Phosphate 2-8wt%, phenol 2-3wt%, bromhexine 2-3wt%, ethyl alcohol 20-40wt%, distilled water 46-74wt%.
4. a kind of sputum specimen film-making colouring method as described in claim 1, which is characterized in that the rotating speed of the centrifugal treating is
1000-1200 turns/min, centrifugation time 2-4min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611229537.0A CN108240932A (en) | 2016-12-27 | 2016-12-27 | A kind of sputum specimen film-making colouring method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611229537.0A CN108240932A (en) | 2016-12-27 | 2016-12-27 | A kind of sputum specimen film-making colouring method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108240932A true CN108240932A (en) | 2018-07-03 |
Family
ID=62702901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611229537.0A Pending CN108240932A (en) | 2016-12-27 | 2016-12-27 | A kind of sputum specimen film-making colouring method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108240932A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235524A (en) * | 2021-12-10 | 2022-03-25 | 江西业力医疗器械有限公司 | Sputum treatment method |
-
2016
- 2016-12-27 CN CN201611229537.0A patent/CN108240932A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114235524A (en) * | 2021-12-10 | 2022-03-25 | 江西业力医疗器械有限公司 | Sputum treatment method |
| CN114235524B (en) * | 2021-12-10 | 2024-02-23 | 南昌准好生物科技有限公司 | Sputum treatment method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180703 |
|
| WD01 | Invention patent application deemed withdrawn after publication |