CN114235524B - Sputum treatment method - Google Patents
Sputum treatment method Download PDFInfo
- Publication number
- CN114235524B CN114235524B CN202111505279.5A CN202111505279A CN114235524B CN 114235524 B CN114235524 B CN 114235524B CN 202111505279 A CN202111505279 A CN 202111505279A CN 114235524 B CN114235524 B CN 114235524B
- Authority
- CN
- China
- Prior art keywords
- sputum
- parts
- mixing kettle
- mixing
- preparing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010036790 Productive cough Diseases 0.000 title claims abstract description 41
- 208000024794 sputum Diseases 0.000 title claims abstract description 41
- 210000003802 sputum Anatomy 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 34
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 27
- 102000004142 Trypsin Human genes 0.000 claims abstract description 17
- 108090000631 Trypsin Proteins 0.000 claims abstract description 17
- 239000011780 sodium chloride Substances 0.000 claims abstract description 17
- 239000012588 trypsin Substances 0.000 claims abstract description 17
- 239000003906 humectant Substances 0.000 claims abstract description 13
- 239000003961 penetration enhancing agent Substances 0.000 claims abstract description 13
- 238000005303 weighing Methods 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000006081 fluorescent whitening agent Substances 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 238000004043 dyeing Methods 0.000 claims abstract description 6
- 239000012530 fluid Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- 238000005282 brightening Methods 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000005484 gravity Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims 3
- 241000233866 Fungi Species 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 17
- 239000012192 staining solution Substances 0.000 description 8
- 239000000975 dye Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention belongs to the technical field of fungus detection, in particular to a sputum treatment method, which aims at the problems that when the sputum is treated by the patients in the existing hospital, the sputum is generally put into a dustbin to cause the spread of some fungi everywhere, thereby causing the spread of various diseases, and the invention provides the following scheme which comprises the following steps: s1: preparing a mixing kettle, a centrifuge, an oven and a weighing instrument; s2: adding trypsin, KOH, sodium chloride and ethanol into a mixing kettle, mixing and stirring uniformly, and preparing a treatment solution; s3: adding a fluorescent whitening agent, a permeation enhancer, a humectant and a background compound dye into a mixing kettle, mixing and stirring uniformly, and preparing a dyeing liquid; s4: and placing the sputum sample into the treatment liquid according to different proportions, and centrifuging by a centrifuge. The invention has simple operation, can reduce the biological infectivity of the sputum sample, can improve the detection rate of fungi and is convenient for people to use.
Description
Technical Field
The invention relates to the technical field of fungus detection, in particular to a sputum treatment method.
Background
Sputum is a liquid which is stimulated and secreted by respiratory tract, and contains mucus, foreign matters, pathogenic microorganisms, various inflammatory cells, necrotic and exfoliated mucosal epithelial cells and the like, and some hospital fluids can be detected aiming at the sputum of patients, so that different symptoms can be obtained.
When the existing hospital patient processes the sputum, the sputum is generally put into a garbage can, so that some fungi are spread everywhere, and various diseases are spread, and a sputum processing method is provided.
Disclosure of Invention
The invention aims to solve the problem that when a patient in a hospital processes sputum, the existing patient generally goes into a garbage can to cause the spread of fungi everywhere, so that the spread of various diseases is caused.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for treating sputum, comprising the steps of:
s1: preparing a mixing kettle, a centrifuge, an oven and a weighing instrument;
s2: adding trypsin, KOH, sodium chloride and ethanol into a mixing kettle, mixing and stirring uniformly, and preparing a treatment solution;
s3: adding a fluorescent whitening agent, a permeation enhancer, a humectant and a background compound dye into a mixing kettle, mixing and stirring uniformly, and preparing a dyeing liquid;
s4: placing the sputum sample into the treatment fluid according to different proportions, centrifuging the sputum sample by a centrifuge,
s5: and (3) dripping the centrifuged sample on a glass slide, drying by a dryer, adding staining solution, covering the stained solution with a cover fragment, and observing by using a microscope.
Preferably, trypsin, KOH, sodium chloride and ethanol are quantified by a weighing instrument in the step S2, and 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodium chloride and 5-6 parts of ethanol are stirred for 5 minutes by a mixing kettle.
Preferably, in the step S2, a weighing instrument is used for quantifying fluorescent brightening agent, penetration enhancer, humectant and background counterstain, wherein 8-10 parts of fluorescent brightening agent, 5-7 parts of penetration enhancer, 3-5 parts of humectant and 2-3 parts of background counterstain are stirred for 3 minutes by a mixing kettle.
Preferably, in the step S4, the sputum is placed in the treatment solution and treated with a centrifuge.
Preferably, the treatment solutions of the sputum with different concentrations in the step S5 are respectively placed on a glass slide, dried by a dryer, treated for 2 minutes by a staining solution, and then respectively microscopic observed by a microscope.
Preferably, the rotating speed of the mixing kettle is 150 revolutions per minute, and a timer is arranged on the mixing kettle.
Preferably, the trypsin, KOH, sodium chloride and ethanol are purified by filtration prior to use.
Preferably, the sputum is placed in equal parts with the treatment fluid according to the ratio of 1/5, 1/10 and 1/20.
Compared with the prior art, the invention has the advantages that:
(1) According to the scheme, the processing liquid is prepared according to a certain proportion, and the sputum sample is digested, so that the biological infectivity of the sputum sample is reduced after digestion, and the detection rate of fungi can be improved;
(2) The fluorescent staining solution is used for detecting fungi, so that the operation is simple, the time consumption is short, the detection rate is high, and the current clinical use is less.
The invention has simple operation, can reduce the biological infectivity of the sputum sample, can improve the detection rate of fungi and is convenient for people to use.
Detailed Description
The technical solutions in this embodiment will be clearly and completely described below, and it is obvious that the described embodiments are only some embodiments of this embodiment, but not all embodiments.
Example 1
A method for treating sputum, comprising the steps of:
s1: preparing a mixing kettle, a centrifuge, an oven and a weighing instrument;
s2: adding trypsin, KOH, sodium chloride and ethanol into a mixing kettle, mixing and stirring uniformly, and preparing a treatment solution;
s3: adding a fluorescent whitening agent, a permeation enhancer, a humectant and a background compound dye into a mixing kettle, mixing and stirring uniformly, and preparing a dyeing liquid;
s4: placing the sputum sample into the treatment fluid according to different proportions, and carrying out centrifugal treatment by a centrifugal machine;
s5: and (3) dripping the centrifuged sample on a glass slide, drying by a dryer, adding staining solution, covering the stained solution with a cover fragment, and observing by using a microscope.
In the embodiment, trypsin, KOH, sodium chloride and ethanol are quantified by a weighing instrument in S2, and 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodium chloride and 5-6 parts of ethanol are stirred for 5 minutes by a mixing kettle.
In the embodiment, the fluorescent whitening agent, the penetration enhancer, the humectant and the background counterstain are quantified by a weighing instrument in the step S2, wherein 8-10 parts of the fluorescent whitening agent, 5-7 parts of the penetration enhancer, 3-5 parts of the humectant and 2-3 parts of the background counterstain are stirred for 3 minutes by a mixing kettle.
In this example, in S4, the sputum is placed in the treatment liquid and treated with a centrifuge.
In this embodiment, the treatment solutions of the sputum with different concentrations in S5 are respectively placed on a glass slide, dried by a dryer, treated for 2 minutes by a staining solution, and then respectively microscopic observed by a microscope.
In this embodiment, the rotational speed of the mixing kettle is 150 rpm, and a timer is provided on the mixing kettle.
In this example, trypsin, KOH, sodium chloride and ethanol were all purified by filtration prior to use.
In this example, the sputum was placed in equal portions with the treatment fluid at 1/5, 1/10 and 1/20 ratios, respectively.
Example two
A method for treating sputum, comprising the steps of:
s1: preparing a mixing kettle, a centrifuge, an oven and a weighing instrument;
s2: adding trypsin, KOH, sodium chloride and ethanol into a mixing kettle, mixing and stirring uniformly, and preparing a treatment solution;
s3: adding a fluorescent whitening agent, a permeation enhancer, a humectant and a background compound dye into a mixing kettle, mixing and stirring uniformly, and preparing a dyeing liquid;
s4: placing the sputum sample into the treatment fluid according to different proportions, centrifuging the sputum sample by a centrifuge,
s5: and (3) dripping the centrifuged sample on a glass slide, drying by a dryer, adding staining solution, covering the stained solution with a cover fragment, and observing by using a microscope.
In this embodiment, in the step S2, trypsin, KOH, sodium chloride and ethanol are quantified by a weighing instrument, 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodium chloride and 5-6 parts of ethanol are stirred for 5 minutes by a mixing kettle, and different treatment solutions can be obtained by materials with different specific gravities, so that the purpose of optimal eliminating phlegm solution can be obtained through experiments.
In this embodiment, in the step S2, a weighing instrument is used to quantify the fluorescent whitening agent, the permeation enhancer, the humectant and the background counterstain, wherein 8-10 parts of the fluorescent whitening agent, 5-7 parts of the permeation enhancer, 3-5 parts of the humectant and 2-3 parts of the background counterstain are stirred for 3 minutes by a mixing kettle, and different staining solutions can be obtained by materials with different specific gravities, so that observation is more obvious when the treated sputum is stained.
In this embodiment, in the step S4, the sputum is placed in the treatment solution, and treated with a centrifuge, and the supernatant is removed from the centrifuged sample, and about 1mL of the bottom solution is left for use, so that the detection is more accurate.
In this embodiment, the treatment solutions of the sputum with different concentrations in S5 are respectively placed on a glass slide, dried by a dryer, treated for 2 minutes by a staining solution, then respectively microscopically observed, and firstly stained by the staining solution, and then observed and detected by a microscope.
In this embodiment, the rotational speed of the mixing kettle is 150 rpm, and a timer is provided on the mixing kettle, and the mixing kettle is clocked by the timer when stirring.
In this example, trypsin, KOH, sodium chloride and ethanol are all purified by filtration before use, so that sputum is more thoroughly treated.
In this embodiment, the sputum is placed in equal parts with the treatment fluid according to the ratio of 1/5, 1/10 and 1/20, so that the treatment effect at different treatment concentrations can be observed.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art will be able to apply equally to the technical solution of the present invention and the inventive concept thereof, within the scope of the present invention.
Claims (1)
1. The sputum treatment method is characterized by comprising the following steps of:
s1: preparing a mixing kettle, a centrifuge, an oven and a weighing instrument;
s2: adding trypsin, KOH, sodium chloride and ethanol into a mixing kettle, mixing and stirring uniformly, and preparing a treatment solution; the trypsin, KOH, sodium chloride and ethanol are purified by filtration before use;
quantifying trypsin, KOH, sodium chloride and ethanol by using a weighing instrument, wherein 10-15 parts of trypsin, 7-10 parts of KOH, 5-7 parts of sodium chloride and 5-6 parts of ethanol are stirred for 5 minutes by using a mixing kettle, and different treatment solutions can be obtained by using materials with different specific gravities;
s3: adding a fluorescent whitening agent, a permeation enhancer, a humectant and a background compound dye into a mixing kettle, mixing and stirring uniformly, and preparing a dyeing liquid;
the fluorescent brightening agent, the penetration enhancer, the humectant and the background compound dye are quantified by a weighing instrument, wherein 8-10 parts of the fluorescent brightening agent, 5-7 parts of the penetration enhancer, 3-5 parts of the humectant and 2-3 parts of the background compound dye are stirred for 3 minutes by a mixing kettle, the rotating speed of the mixing kettle is 150 revolutions per minute, a timer is arranged on the mixing kettle, and the timer is used for timing when the mixing kettle is used for stirring; different dyeing liquids can be obtained through materials with different specific gravities;
s4: placing a sputum sample into a treatment solution according to different proportions, centrifuging the sputum sample by a centrifuge, pouring out supernatant from the centrifuged sample, and reserving about 1mL of bottom solution for later use;
s5: respectively dripping the centrifuged treatment fluid samples of sputum with different concentrations on a glass slide, drying the glass slide by a dryer, adding the staining fluid, covering the glass slide with the staining fluid, treating the glass slide for 2 minutes by the staining fluid, and carrying out microscopic observation by a microscope; the sputum is placed in equal parts with the treatment fluid according to the proportion of 1/5, 1/10 and 1/20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111505279.5A CN114235524B (en) | 2021-12-10 | 2021-12-10 | Sputum treatment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111505279.5A CN114235524B (en) | 2021-12-10 | 2021-12-10 | Sputum treatment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114235524A CN114235524A (en) | 2022-03-25 |
| CN114235524B true CN114235524B (en) | 2024-02-23 |
Family
ID=80754623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111505279.5A Active CN114235524B (en) | 2021-12-10 | 2021-12-10 | Sputum treatment method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114235524B (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030094901A (en) * | 2002-06-10 | 2003-12-18 | 김종기 | Early detection of lung cancer using acridine staining and fluorescence sputum cytology |
| CN101988875A (en) * | 2010-09-03 | 2011-03-23 | 江苏省原子医学研究所 | Sputum liquid-based treating fluid |
| CN106868090A (en) * | 2017-03-28 | 2017-06-20 | 王绪友 | A kind of medical test sputum processing method |
| CN108240932A (en) * | 2016-12-27 | 2018-07-03 | 孝感市森茂激光数控设备有限公司 | A kind of sputum specimen film-making colouring method |
| CN108240928A (en) * | 2016-12-27 | 2018-07-03 | 孝感市森茂激光数控设备有限公司 | A kind of sputum specimen treatment fluid and preparation method thereof |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
| CN109358028A (en) * | 2018-11-09 | 2019-02-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology and fluorescent staining technique |
| CN109374376A (en) * | 2018-11-09 | 2019-02-22 | 上海市农业科学院 | A kind of slice preparation method suitable for mushroom lamella Basidium morphologic observation |
| CN111289336A (en) * | 2019-12-13 | 2020-06-16 | 山东源科生物科技股份有限公司 | Fungus fluorescent staining solution and preparation method thereof |
| CN111380736A (en) * | 2018-12-29 | 2020-07-07 | 江西业力医疗器械有限公司 | Fluorescent staining solution preparation method and fluorescent staining solution |
| CN111521471A (en) * | 2020-05-12 | 2020-08-11 | 安徽京和生物药业科技有限公司 | Fluorescent staining solution for detecting fungi D-glucan and preparation method thereof |
| JP6879589B1 (en) * | 2019-11-26 | 2021-06-02 | ミテジマ化学株式会社 | Anti-yellowing agent to prevent yellowing of fluorescent whitening agent and anti-yellowing technology using it |
| CN113575573A (en) * | 2021-09-07 | 2021-11-02 | 易普森生物科技(深圳)有限公司 | Preservation solution for sputum sample and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100642838B1 (en) * | 2000-06-13 | 2006-11-13 | 김인규 | A method for manufacturing an active component of surfactant, surfactant and a method for using the surfactant |
-
2021
- 2021-12-10 CN CN202111505279.5A patent/CN114235524B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030094901A (en) * | 2002-06-10 | 2003-12-18 | 김종기 | Early detection of lung cancer using acridine staining and fluorescence sputum cytology |
| CN101988875A (en) * | 2010-09-03 | 2011-03-23 | 江苏省原子医学研究所 | Sputum liquid-based treating fluid |
| CN108240932A (en) * | 2016-12-27 | 2018-07-03 | 孝感市森茂激光数控设备有限公司 | A kind of sputum specimen film-making colouring method |
| CN108240928A (en) * | 2016-12-27 | 2018-07-03 | 孝感市森茂激光数控设备有限公司 | A kind of sputum specimen treatment fluid and preparation method thereof |
| CN106868090A (en) * | 2017-03-28 | 2017-06-20 | 王绪友 | A kind of medical test sputum processing method |
| CN109297790A (en) * | 2018-10-26 | 2019-02-01 | 江西业力医疗器械有限公司 | A kind of fluorescent staining liquid detecting fungi |
| CN109358028A (en) * | 2018-11-09 | 2019-02-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology and fluorescent staining technique |
| CN109374376A (en) * | 2018-11-09 | 2019-02-22 | 上海市农业科学院 | A kind of slice preparation method suitable for mushroom lamella Basidium morphologic observation |
| CN111380736A (en) * | 2018-12-29 | 2020-07-07 | 江西业力医疗器械有限公司 | Fluorescent staining solution preparation method and fluorescent staining solution |
| JP6879589B1 (en) * | 2019-11-26 | 2021-06-02 | ミテジマ化学株式会社 | Anti-yellowing agent to prevent yellowing of fluorescent whitening agent and anti-yellowing technology using it |
| CN111289336A (en) * | 2019-12-13 | 2020-06-16 | 山东源科生物科技股份有限公司 | Fungus fluorescent staining solution and preparation method thereof |
| CN111521471A (en) * | 2020-05-12 | 2020-08-11 | 安徽京和生物药业科技有限公司 | Fluorescent staining solution for detecting fungi D-glucan and preparation method thereof |
| CN113575573A (en) * | 2021-09-07 | 2021-11-02 | 易普森生物科技(深圳)有限公司 | Preservation solution for sputum sample and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| RNA恒温扩增实时荧光检测技术对复治菌阳肺结核患者疗效评价的研究;耿书军;刘建玲;党萍;马清艳;侯莉莉;康冠楠;宋韬;;天津医药(第09期);全文 * |
| 痰液细菌学检验的标准化操作程序初探;杨进;卢先雷;罗宇鹏;;医学检验与临床(第02期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114235524A (en) | 2022-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103145878B (en) | A kind of ultralow point of heparin sodium technical study | |
| CN1967196A (en) | Simplified thin-layer liquid-based cytology detection method | |
| CN102393318A (en) | Simple and quick preparation method of thin-layer liquid-based cytology cell smear | |
| CN114235524B (en) | Sputum treatment method | |
| CN206546353U (en) | A kind of COD titration outfit | |
| CN106620067A (en) | Method for extracting pineapple peel flavonoid compound | |
| CN112662731A (en) | Coronavirus sample preservation solution and application thereof | |
| CN207805157U (en) | It is circulating be filtered, washed, drying system | |
| CN208649176U (en) | It is a kind of for extracting the separating pipe of high concentration growth factor | |
| WO2012037719A2 (en) | PREPARATION AND USING METHOD OF KIT FOR COLORIMETRIC DETECTING FUNGAL (1-3)-β -D-GLUCAN | |
| CN112574938B (en) | Sputum treatment fluid for membrane filtration enriched bacteria and application thereof | |
| CN109001455B (en) | Alveolar lavage fluid sample treatment fluid and treatment detection method | |
| CN111579538A (en) | Apoptosis kit applied to circulating tumor cell detection and detection method thereof | |
| CN109668771B (en) | Liquid-based exfoliative cell staining solution and staining method thereof | |
| CN102628071B (en) | Preparation method of decolorized squid skin collagen | |
| CN115993277A (en) | Sputum treatment method for medical examination | |
| CN111172153A (en) | Human urine exosome nucleic acid extraction method | |
| CN111238890A (en) | Detection method for liquid-based fungi by using liquid-based flaking technology based on centrifugation method | |
| CN109234382A (en) | The fluorescent quantificationally PCR detecting kit and its method of pyemia infection conditions are judged in advance | |
| CN111269839B (en) | Equilibrium liquid for enriching pathogenic bacteria and preparation method and application thereof | |
| CN220188121U (en) | Peritoneal dialysis liquid sample collection device | |
| CN115960988A (en) | Solution and method for sample pretreatment | |
| CN113358447B (en) | Fungus cell positive wax block and preparation method and application thereof | |
| CN115806744B (en) | Method for preparing blue dye by extracting tannin from dogwood and device used by method | |
| Wang et al. | Extraction of garlic essential oil with electrospun nanofibers and its antioxidant activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240122 Address after: Room 306, Building 123, Phase II, Nanchang Jiahai Industrial Park, No. 2799 Tianxiang Avenue, Nanchang High tech Industrial Development Zone, Nanchang City, Jiangxi Province, 330000 Applicant after: Nanchang Zhunhao Biotechnology Co.,Ltd. Country or region after: China Address before: Room 123, phase II, Nanchang Jiahai Industrial Park, 2799 Tianxiang Avenue, Nanchang high tech Industrial Development Zone, Jiangxi Province Applicant before: JIANGXI YELI MEDICAL INSTRUMENT CO.,LTD. Country or region before: China |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |