CN101988875A - Sputum liquid-based treating fluid - Google Patents
Sputum liquid-based treating fluid Download PDFInfo
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- CN101988875A CN101988875A CN 201010271881 CN201010271881A CN101988875A CN 101988875 A CN101988875 A CN 101988875A CN 201010271881 CN201010271881 CN 201010271881 CN 201010271881 A CN201010271881 A CN 201010271881A CN 101988875 A CN101988875 A CN 101988875A
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- mucus
- treating fluid
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- sputum
- liquid base
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Abstract
The invention relates to sputum liquid-based treating fluid, belonging to the technical field of medicament. The sputum liquid-based treating fluid is used by a membrane-type liquid-based thin cell pelleter and consists of alcohol, double distilled water, sodium chloride, phosphate, sterilizing agent phenol, mucus decomposition agent and mucus decomposition peptide. The invention has the advantages that: a sample is directly collected to the treating fluid to be subjected to oscillation treatment to completely digest sputum, meanwhile, the completeness of cell form can be maintained, and the cells with diagnosis value are collected to the maximum limit so as to effectively remove impurities of proteins, mucus, and the like which influences diagnosis in the sample, the cells are directly palletized on the machine, and the smear backgrounds of the manufactured thin cells are clean to benefit the diagnosis of tumor cells.
Description
Technical field
The invention belongs to the medicament technical field, relate to a kind of sputum liquid base treating fluid, for the supporting use of membrane liquid base thin-layer cell pelleter film-making.
Background technology
In China, lung cancer is first cancer, surpasses 20% of the cancer cause of the death, and the incidence of disease and mortality ratio increase rapidly.Clinical stages when prognosis of lung cancer and diagnosis is closely related, 5 years survival rates of 0 phase patients with lung cancer postoperative can reach more than 90%, 5 years survival rates of Ia phase patients with lung cancer postoperative are 60%, and 5 years total survival rates of II～IV phase patient then drop to below 5% from 40%.Therefore, striving for " early detection, early diagnosis, early treatment ", is the important measures that reduce lung cancer mortality.From nineteen thirty, phlegm cytology checking is applied to the diagnosis of lung cancer.Phlegm cytology checking, particularly repeatedly phlegm inspection originate from the endosteoma of air pipe to diagnosis, are helpful as squama cancer and small cell carcinoma.In recent years, liquid basal cell learns a skill and is applied to the phlegm inspection, and all more traditional method is improved on the cell separation smear of drawing materials, background and eucaryotic cell structure are observed.Diagnostic accuracy of reporting in the lung cancer case and specificity then surpass 90%.
The sputum sample is thickness very, and cell content is few, be the complete stripping of the cell that diagnostic value is arranged, the general clinically cleaning fluid that adds earlier vibrate again cellular layer to be transferred to preserve after centrifugal and goes up machine-processed sheet in the liquid, generally also needs repeatedly to clean and vibrates, complex operation has brought inconvenience to testing.
Summary of the invention
For addressing the above problem, the invention provides a kind of sputum liquid base treating fluid, mucus is decomposed peptide add in the sputum treating fluid, sample is directly gathered to treating fluid, and sample is after oscillation treatment, but the catapepsis sputum, keep simultaneously the complete of cellular morphology again, the processing time is short, and is simple to operate.
It is a kind of neutral mucopolysacchauide peptide that extracts from animal tracheae, soft connective tissue that mucus is decomposed peptide, and this kind mucopolysaccharide peptide 85% is a Type B, and all the other 10% are A type and C type.It is white crystalline powder, odorless that mucus is decomposed peptide, and physicochemical property is stable, and its pharmacological function is for quickening the decomposition of respiratory mucus, relief of symptoms.
The present invention uses mucus to decompose in the peptide adding sputum treating fluid, sample is directly gathered to treating fluid, after oscillation treatment, but the catapepsis sputum keeps the complete of cellular morphology simultaneously again, collects the cell that diagnostic value is arranged to greatest extent, impurity such as the albumen of influence diagnosis, mucus in effective removal sample, directly go up machine-processed sheet, the thin-layer cell smear background of making is clean, helps the diagnosis of tumour cell.
Technical scheme of the present invention is: a kind of sputum liquid base treating fluid, it is characterized in that: form by alcohols, distilled water, sodium chloride, phosphate, bactericidal agent phenol and mucus distintegrant, alcohols accounts for the 15%-30% for the treatment of fluid cumulative volume, and distilled water replenishes not enough treating fluid volume; Described sputum liquid base treating fluid is by 100 milliliters of calculating, and wherein sodium chloride content is that 0.5-2.5 gram, phosphate content are the 0.1-3.0 gram, and phenol content is 1.0 grams, and mucus distintegrant content is the 1.0-3.0 gram; Wherein, described alcohols is selected a kind of in methyl alcohol, ethanol, propyl alcohol, the isopropyl alcohol or any two kinds for use, and mass concentration is 95%-100%; Described mucus distintegrant is that mucus is decomposed peptide; Described phosphate is selected any one in sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium phosphate, dipotassium hydrogen phosphate, the potassium dihydrogen phosphate for use.The optium concentration of mucus distintegrant in sputum liquid base treating fluid is: contain 2.0 gram mucus distintegrants in per 100 milliliters of sputum liquid base treating fluids.
Beneficial effect of the present invention is:
1, reduces cost greatly.About 150 yuan/cover of the supporting consumptive material price of import, consumables cost of the present invention is about 20 yuan;
2, add unique mucus distintegrant mucus and decompose peptide, can successfully separate the cell that is wrapped up by mucus in the sample, avoided positive cell to pile up and lose, thereby improved effective cell quantity;
3, add bactericidal agent phenol, can kill pathogen such as mould, bacterium, virus effectively, protection working environment and staff's health;
4, suitable phosphate buffering liquid concentration can guarantee the cell attitude that holds its shape, and fixing under the effect of alcohols, made sample is preserved all no changes.Thereby provide a kind of with low cost, the sputum liquid base treating fluid that result of use is good, use the sputum liquid base treating fluid of the present invention's development, carry out that the smear background that automatic pelleter film-making makes is Clear ﹠ Transparent, cell monolayer evenly distributes, and is convenient to the doctor's of pathology department the sheet of reading.
Description of drawings
Fig. 1 handles the thin-layer cell smear of making behind the sputum sample for treating fluid of the present invention.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for description and interpretation the present invention, and be not used in qualification the present invention.
Embodiment 1
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Methyl alcohol 200mL
Sodium chloride 10.0g
Sodium phosphate 10.0g
Phenol 10.0g
Mucus is decomposed peptide 20.0g
Distilled water 800mL
Embodiment 2
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Methyl alcohol 150mL
Sodium chloride 15.0g
Sodium dihydrogen phosphate 5.0g
Phenol 10.0g
Mucus is decomposed peptide 10.0g
Distilled water 850mL
Embodiment 3
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Ethanol 150mL
Sodium chloride 15.0g
Sodium hydrogen phosphate 5.0g
Phenol 10.0g
Mucus is decomposed peptide 10.0g
Distilled water 850mL
Embodiment 4
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Methyl alcohol 100mL
Ethanol 100mL
Sodium chloride 10.0g
Potassium phosphate 8.0g
Phenol 10.0g
Mucus is decomposed peptide 20.0g
Distilled water 800mL
Embodiment 5
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Isopropyl alcohol 200mL
Sodium chloride 6.0g
Potassium dihydrogen phosphate 8.0g
Phenol 10.0g
Mucus is decomposed peptide 15.0g
Distilled water 800mL
Embodiment 6
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Methyl alcohol 300mL
Sodium chloride 10.0g
Dipotassium hydrogen phosphate 10.0g
Phenol 10.0g
Mucus is decomposed peptide 20.0g
Distilled water 700mL
Embodiment 7
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Propyl alcohol 300mL
Sodium chloride 25.0g
Dipotassium hydrogen phosphate 30.0g
Phenol 10.0g
Mucus is decomposed peptide 30.0g
Distilled water 700mL
Embodiment 8
The compound method of sputum liquid base treating fluid of the present invention is: the weighing of according to the form below composition and measure after, stirring and dissolving gets final product.
Methyl alcohol 300mL
Sodium chloride 5.0g
Dipotassium hydrogen phosphate 1.0g
Phenol 10.0g
Mucus is decomposed peptide 10.0g
Distilled water 700mL
Embodiment 9
The sputum sample is directly gathered to the 20ml treating fluid, after oscillation treatment, the catapepsis of sputum sample, keep simultaneously the complete of cellular morphology again, collect the cell that diagnostic value is arranged to greatest extent, impurity such as the albumen of influence diagnosis, mucus in effective removal sample, adopt the import automatic pelleter of new Bai Shi liquid based thin-layer cell or state's automatic pelleter of production fluid based thin-layer cell to carry out film-making, the thin-layer cell smear of making, as shown in Figure 1, background is Clear ﹠ Transparent, cell monolayer evenly distributes, and is convenient to the doctor's of pathology department the sheet of reading, and helps the diagnosis of tumour cell.The smear background that makes is Clear ﹠ Transparent, cell monolayer evenly distributes.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. sputum liquid base treating fluid, it is characterized in that: be made up of alcohols, distilled water, sodium chloride, phosphate, bactericidal agent phenol and mucus distintegrant, alcohols accounts for the 15%-30% for the treatment of fluid cumulative volume, and distilled water replenishes not enough treating fluid volume;
Described sputum liquid base treating fluid is by 100 milliliters of calculating, and wherein sodium chloride content is that 0.5-2.5 gram, phosphate content are the 0.1-3.0 gram, and phenol content is 1.0 grams, and mucus distintegrant content is the 1.0-3.0 gram;
Described alcohols is a kind of in methyl alcohol, ethanol, propyl alcohol, the isopropyl alcohol or any two kinds, and mass concentration is 95%-100%;
Described mucus distintegrant is that mucus is decomposed peptide;
Described phosphate is any one in sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium phosphate, dipotassium hydrogen phosphate, the potassium dihydrogen phosphate.
2. a kind of sputum liquid base treating fluid according to claim 1 is characterized in that the concentration of described mucus distintegrant in sputum liquid base treating fluid is: contain 2.0 gram mucus distintegrants in per 100 milliliters of sputum liquid base treating fluids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010271881 CN101988875A (en) | 2010-09-03 | 2010-09-03 | Sputum liquid-based treating fluid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010271881 CN101988875A (en) | 2010-09-03 | 2010-09-03 | Sputum liquid-based treating fluid |
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| CN101988875A true CN101988875A (en) | 2011-03-23 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822815A (en) * | 2014-03-25 | 2014-05-28 | 吉安协和医院 | Method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears |
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN105092328A (en) * | 2015-07-30 | 2015-11-25 | 厦门宝太生物科技有限公司 | Erythrocyte removing treating fluid and application |
| CN105779559A (en) * | 2016-03-28 | 2016-07-20 | 天津智达康华生物科技有限公司 | Liquid-based cell treatment solution |
| CN107258765A (en) * | 2016-04-08 | 2017-10-20 | 杨珍杰 | Sputum preserves liquid and preparation method thereof |
| CN110243643A (en) * | 2019-06-24 | 2019-09-17 | 杨美菊 | A kind of patients with lung cancer initial stage bloodysputum liquid detection separator based on vibration sieve method |
| CN113575573A (en) * | 2021-09-07 | 2021-11-02 | 易普森生物科技(深圳)有限公司 | Preservation solution for sputum sample and application thereof |
| CN114235524A (en) * | 2021-12-10 | 2022-03-25 | 江西业力医疗器械有限公司 | Sputum treatment method |
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| CN1320819A (en) * | 2000-04-26 | 2001-11-07 | 鞍山钢铁集团公司 | Phlegm-treating agent for examining cancer cells |
| WO2002078636A2 (en) * | 2001-03-28 | 2002-10-10 | Matritech, Inc. | Detection and treatment of colorectal cancer |
| CN201091587Y (en) * | 2007-10-31 | 2008-07-30 | 邢炜东 | Sputum treatment sputum collection device for respiratory inspection |
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2010
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Patent Citations (4)
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| CN1320819A (en) * | 2000-04-26 | 2001-11-07 | 鞍山钢铁集团公司 | Phlegm-treating agent for examining cancer cells |
| WO2002078636A2 (en) * | 2001-03-28 | 2002-10-10 | Matritech, Inc. | Detection and treatment of colorectal cancer |
| US20090326209A1 (en) * | 2004-12-01 | 2009-12-31 | Weiwei Li | Method for isolating and modifying DNA from blood and body fluids |
| CN201091587Y (en) * | 2007-10-31 | 2008-07-30 | 邢炜东 | Sputum treatment sputum collection device for respiratory inspection |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN103822815A (en) * | 2014-03-25 | 2014-05-28 | 吉安协和医院 | Method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears |
| CN105092328A (en) * | 2015-07-30 | 2015-11-25 | 厦门宝太生物科技有限公司 | Erythrocyte removing treating fluid and application |
| CN105779559A (en) * | 2016-03-28 | 2016-07-20 | 天津智达康华生物科技有限公司 | Liquid-based cell treatment solution |
| CN107258765A (en) * | 2016-04-08 | 2017-10-20 | 杨珍杰 | Sputum preserves liquid and preparation method thereof |
| CN110243643A (en) * | 2019-06-24 | 2019-09-17 | 杨美菊 | A kind of patients with lung cancer initial stage bloodysputum liquid detection separator based on vibration sieve method |
| CN110243643B (en) * | 2019-06-24 | 2021-09-03 | 赵金 | Lung cancer patient early stage takes blood sputum to detect and uses separator based on sieve method shakes |
| CN113575573A (en) * | 2021-09-07 | 2021-11-02 | 易普森生物科技(深圳)有限公司 | Preservation solution for sputum sample and application thereof |
| CN114235524A (en) * | 2021-12-10 | 2022-03-25 | 江西业力医疗器械有限公司 | Sputum treatment method |
| CN114235524B (en) * | 2021-12-10 | 2024-02-23 | 南昌准好生物科技有限公司 | Sputum treatment method |
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Application publication date: 20110323 |