CN106124276A - A kind of blood coagulation accelerator and preparation method thereof - Google Patents
A kind of blood coagulation accelerator and preparation method thereof Download PDFInfo
- Publication number
- CN106124276A CN106124276A CN201610730531.5A CN201610730531A CN106124276A CN 106124276 A CN106124276 A CN 106124276A CN 201610730531 A CN201610730531 A CN 201610730531A CN 106124276 A CN106124276 A CN 106124276A
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- polysorbate
- blood coagulation
- powder
- coagulation accelerator
- isopropanol
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of blood coagulation accelerator and preparation method thereof, it includes α hydrogen ω hydroxyl (oxygen 1,2 second diyls) polymer, polyvinylpyrrolidone, Polysorbate, polysorbate, SiO 2 powder and isopropanol, coagulant obtained by the present invention can effectively shorten the time of blood coagulation, blood wall cling phenomenon will not be formed, without interference with the biochemical and result of immunological experiment detection, effect duration is long, possesses the performance of excellence.
Description
Technical field
The invention belongs to the technical field of blood coagulation accelerator, relate to a kind of blood coagulation accelerator and preparation method thereof.
Background technology
Serum, after referring to blood coagulation, removes the isolated light yellow transparent liquid of Fibrinogen in blood plasma, and it is main
Effect be to provide basic nutrition material, provide hormone and various somatomedin, provide associated proteins, provide promote to contact and stretch because of
Son make cell attachment from mechanical damage, to cultivate in cell play some protective effect, be clinical biochemical, immunity etc. try
One of main specimen testing detection.Generally, in vitro after blood preparation need could solidify completely for more than 60 minutes, difficult
To meet the demand that laboratory quickly detects.
At present clinically when carrying out biochemical index, in blood, addition coagulant is to shorten clotting time, tradition
Coagulant be kaolin, cephalin and thrombin, but first two become branch to testing result produce interference, cause detection knot
The most inaccurate, thrombin needs to be equipped with special equipment, and cost is big.
Summary of the invention
It is an object of the invention to overcome the defect of prior art, it is provided that it is a kind of that clotting time is short, do not disturb inspection result,
The blood coagulation accelerator of excellent performance, and its preparation method is provided simultaneously.
To achieve these goals, the technical scheme that the present invention takes is as follows:
One aspect of the present invention provides a kind of blood coagulation accelerator, and it includes the polymerization of α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl)
Thing, polyvinylpyrrolidone, Polysorbate, polysorbate, SiO 2 powder and isopropanol.
Further improve as the present invention, the polymer of described α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl), poly-Pyrusussuriensis
The volume ratio of ester, polysorbate and isopropanol is 1 ~ 3:40 ~ 80:20 ~ 40:1000；Described polyvinylpyrrolidone and isopropanol
Mass volume ratio be 2 ~ 5g:1000ml；The mass volume ratio of described SiO 2 powder and described isopropanol is 6-15g:
1000ml。
Another aspect of the present invention provides the preparation method of a kind of above-mentioned blood coagulation accelerator, and it comprises the steps:
(1) polymer, polyvinylpyrrolidone and the isopropanol of α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl) are mixed in proportion
Close, use high shear dispersion device fully to mix, until mixed solution clear, obtain mixed liquor A；
(2) Polysorbate, polysorbate and SiO 2 powder are proportionally mixed, stirring, then use high pressure homogenizer
Carry out homogenizing, obtain mixture B；
(3) the mixture B that mixed liquor A step (1) obtained and step (2) obtain mixes, and shakes up,.
Further improving as the present invention, in described step (1), the rotating speed of high shear dispersion device is 4000 revs/min.
Further improving as the present invention, use high pressure homogenizer to carry out homogenizing in described step (2), concrete operations are
First with two-stage pressurizing, when being added to 180bar ~ 220bar, pressurize by one-level the most again, be forced into what required abrasive media needed
Pressure, 280bar ~ 350bar, mixture circulates 3 times in homogenizer, and homogenizing obtains mixture B after terminating.
Further improve as the present invention, by the homogenizing of described step (2) make SiO 2 powder and Polysorbate,
Polysorbate fully mixes and makes SiO 2 powder reach nanoscale.
Compared with prior art, having the beneficial effect that acquired by the present invention:
Polymer and the polyvinylpyrrolidone of the α of the present invention-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl) can be formed bionical
Film, the generation of anti-Hemostatic Oral Liquid wall cling phenomenon.The SiO 2 powder of the present invention can effectively facilitate blood coagulation.The present invention's is poly-
Pyrusussuriensis ester can help silicon dioxide to disperse, with test tube laminating uniformly.Polysorbate can make each composition mix evenly, simultaneously
Reduce the possibility of suspension fiber Protein filament in serum.Coagulant obtained by the present invention can effectively shorten blood coagulation time
Between, blood wall cling phenomenon will not be formed, without interference with the biochemical and result of immunological experiment detection, effect duration is long, possesses excellence
Performance.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, invention is carried out
Clear, complete description.
Embodiment 1
Measure polymer, 3.5g polyvinylpyrrolidone and the 1000ml isopropyl of 2ml α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl)
Alcohol, mixing, use high shear dispersion device fully to mix, until mixed solution clear, obtain mixed liquor A；
Measure 60ml Polysorbate, 30ml polysorbate and the mixing of 10.5g SiO 2 powder, stirring, then use high pressure equal
Matter machine carries out three homogenizing, obtains mixture B；
(3) the mixture B that mixed liquor A step (1) obtained and step (2) obtain mixes, and shakes up,.
Embodiment 2
Measure polymer, 3g polyvinylpyrrolidone and the 1000ml isopropyl of 3ml α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl)
Alcohol, mixing, use high shear dispersion device fully to mix, until mixed solution clear, obtain mixed liquor A；
Measure 80ml Polysorbate, 40ml polysorbate and the mixing of 6g SiO 2 powder, stirring, then use high pressure homogenizer
Carry out three homogenizing, obtain mixture B；
(3) the mixture B that mixed liquor A step (1) obtained and step (2) obtain mixes, and shakes up,.
Embodiment 3
Measure polymer, 5g polyvinylpyrrolidone and the 1000ml isopropyl of 1ml α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl)
Alcohol, mixing, use high shear dispersion device fully to mix, until mixed solution clear, obtain mixed liquor A；
Measure 40ml Polysorbate, 20ml polysorbate and the mixing of 15g SiO 2 powder, stirring, then use high pressure homogenize
Machine carries out three homogenizing, obtains mixture B；
(3) the mixture B that mixed liquor A step (1) obtained and step (2) obtain mixes, and shakes up,.
Effect example
The coagulant that Example 1,2 and 3 obtains respectively is sprayed at blood collecting containers inwall, and each blood taking tube sprays 25 μ L, then
With hot-air, it is dried, carry out vacuum tamponade according to the specification of 5ml, complete the manufacture of blood taking tube, in case follow-up use.
Using ready blood taking tube to gather venous blood specimen, every blood taking tube gathers 5ml, reverse mixing 5 after blood sampling
Secondary, upright placement 15 minutes, within 5 minutes, prepare serum with the relative centrifugal force of 1700gn, result is as follows:
The above embodiment is only the preferred embodiments of the present invention, and and the feasible enforcement of non-invention exhaustive.For this
For skilled person, on the premise of without departing substantially from the principle of the invention and spirit to its done any obviously
Change, within all should being contemplated as falling with the claims of the present invention.
Claims (6)
1. a blood coagulation accelerator, it is characterised in that it includes the polymer of α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl), gathers
Vinylpyrrolidone, Polysorbate, polysorbate, SiO 2 powder and isopropanol.
A kind of blood coagulation accelerator the most according to claim 1, it is characterised in that described α-hydrogen-ω-hydroxyl (oxygen-1,2-second
Diyl) the volume ratio of polymer, Polysorbate, polysorbate and isopropanol be 1 ~ 3:40 ~ 80:20 ~ 40:1000；Described poly-
The mass volume ratio of vinylpyrrolidone and isopropanol is 2 ~ 5g:1000ml；Described SiO 2 powder and described isopropanol
Mass volume ratio is 6-15g:1000ml.
3. the preparation method of a blood coagulation accelerator as claimed in claim 1 or 2, it is characterised in that it comprises the steps:
(1) polymer, polyvinylpyrrolidone and the isopropanol of α-hydrogen-ω-hydroxyl (oxygen-1,2-second diyl) are mixed in proportion
Close, use high shear dispersion device fully to mix, until mixed solution clear, obtain mixed liquor A；
(2) Polysorbate, polysorbate and SiO 2 powder are proportionally mixed, stirring, then use high pressure homogenizer
Carry out homogenizing, obtain mixture B；
(3) the mixture B that mixed liquor A step (1) obtained and step (2) obtain mixes, and shakes up,.
The preparation method of a kind of blood coagulation accelerator the most according to claim 3, it is characterised in that high in described step (1)
The rotating speed of shearing dispersing device is 4000 revs/min.
The preparation method of a kind of blood coagulation accelerator the most according to claim 3, it is characterised in that described step makes in (2)
Carrying out homogenizing with high pressure homogenizer, concrete operations are first with two-stage pressurizing, when being added to 180bar ~ 220bar, use one-level the most again
Pressurization, is forced into the pressure that required abrasive media needs, 280bar ~ 350bar, and mixture circulates 3 times in homogenizer, all
Matter obtains mixture B after terminating.
The preparation method of a kind of blood coagulation accelerator the most according to claim 3, it is characterised in that by described step (2)
Homogenizing make SiO 2 powder and Polysorbate, Polysorbate fully mix and make SiO 2 powder reach nanoscale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610730531.5A CN106124276A (en) | 2016-08-26 | 2016-08-26 | A kind of blood coagulation accelerator and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610730531.5A CN106124276A (en) | 2016-08-26 | 2016-08-26 | A kind of blood coagulation accelerator and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN106124276A true CN106124276A (en) | 2016-11-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610730531.5A Pending CN106124276A (en) | 2016-08-26 | 2016-08-26 | A kind of blood coagulation accelerator and preparation method thereof |
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| Country | Link |
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| CN (1) | CN106124276A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751186A (en) * | 2020-07-09 | 2020-10-09 | 威海威高采血耗材有限公司 | Blood coagulation accelerator for blood collection tube and preparation method thereof |
-
2016
- 2016-08-26 CN CN201610730531.5A patent/CN106124276A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751186A (en) * | 2020-07-09 | 2020-10-09 | 威海威高采血耗材有限公司 | Blood coagulation accelerator for blood collection tube and preparation method thereof |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161116 |