CN108208537A - A kind of method for releasing aflatoxin B1 - Google Patents
A kind of method for releasing aflatoxin B1 Download PDFInfo
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- CN108208537A CN108208537A CN201810051502.5A CN201810051502A CN108208537A CN 108208537 A CN108208537 A CN 108208537A CN 201810051502 A CN201810051502 A CN 201810051502A CN 108208537 A CN108208537 A CN 108208537A
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- Prior art keywords
- aflatoxin
- composite
- antidote
- corn
- carrier granular
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
Abstract
The present invention by buckwheat shell, lotus rhizome slag by carrying out extrusion processing; it is crushed to the puffing carrier granular less than 1mm diameters later; puffing carrier granular is added in the zymotic fluid of Methylotrophic bacillus; it is made to adsorb a certain proportion of zymotic fluid and obtains composite antidote; composite antidote is uniformly mixed with the niblet that aspergillus flavus pollutes; the aflatoxin B1 (AFB1) in mouldy corn can efficiently be released, the releasing rate of the aflatoxin B1 in mouldy corn reaches more than 70%.When action pH is 5~6 mouldy corn, individually released in corn compared with the efficiency of AFB1 with Methylotrophic bacillus bacterium solution under equal conditions, composite antidote can promote 20%~40% to the releasing rate of AFB1.Composite antidote can keep more preferable stability, still have stronger detoxification ability after recycling three times.The present invention has wide application prospect in the AFB1 in releasing corn.
Description
Technical field
The invention belongs to probiotics fields, and in particular to a kind of composite antidote, it will be by the buckwheat of extrusion
Shell and the compound Methylotrophic fermentation of bacillus liquid of lotus rhizome slag carrier granular form, this composite antidote can efficiently release Huang
Aspertoxin B1.
Background technology
Buckwheat shell is simply discarded as a kind of agricultural by-products, most buckwheat shell, can many times be incinerated place
Reason forms environmental pollution, causes the great wasting of resources.Lotus rhizome slag is lotus rhizome treated leftover bits and pieces, many lotus root products processing
Lead to the pollution of environment containing lotus root slag, the discarded of a large amount of lotus root slags in waste material and restrict economy.Cao Danfeng is with citric acid to buckwheat
Shell carries out chemical modification, finds modified buckwheat shell adsorbent to Cu2+ Adsorbance increase (Cao Danfeng, 2014).Li Kebin with
Agricultural wastes buckwheat skin removes Cr (VI) (Li Kebin, 2012) in water removal as biological adsorption agent.By using 1- hydroxy ethylidenes two
Phosphonic acids（HEDP）Functionalization agricultural residual buckwheat shell（BH）, the new bio sorbing material absorption heavy metal such as Au of acquisition（III）
(Ping Yin, 2012).These researchers focus on studying buckwheat shell or being modified buckwheat shell makes it act on heavy metal element,
Play the suction-operated of buckwheat shell material counterweight metal pollutant.However, being handled using chemical reagent, buckwheat shell can be caused in itself
Pollution and pure in security risk.Niu Weiwei using lotus rhizome slag as material, answered by cereal common with extrusion, potato starch
Match, result of study show by lotus rhizome slag and cornstarch compounding for extrusion processing in, can produce form, tissue and
Leisure dilated food (Niu Weiwei, 2013) good in taste.
Nature has many fungies and bacterium can adsorb or degrading aflatoxin B 1, we are by a large amount of screening work
Make, it was found that one plant of Methylotrophic bacillus, and verify that it has degradation to aflatoxin B1.Buckwheat shell and lotus
Carrier granular prepared by lotus root slag have special construction can by bacterium solution stable bond, some researches show that magnetic macromolecular microsphere have than
The advantages that surface area is big, grain size is small becomes new Shanxi carrier (Deng Tao, 2013), by extrusion handle buckwheat shell and lotus rhizome slag it
Afterwards, it has been found that the number of the carrier granular mesoporous of preparation increases, and specific surface area increases.Dawn sherry is opened to be inhaled with HSCAS
Attached aspergillus niger FS-UV1 spore suspensions, by the HSCAS for combining aspergillus niger for the degradation of aflatoxin B1 and to Cottonseed Meal
In AFB1 detoxification, and show to be used for multiple times after immobilization FS-UV1, when 48 h reaches the removal efficiency of AFB1
More than 60%, still have active well (Zhang Xiaoxue, 2015).Carrier granular in composite antidote of the present invention can will detoxify
Microorganism and detoxication enzyme are strong bonded, show the microorganism after being combined with carrier by experiment and its product improve it is active and
Patience has no effect on its detoxification ability in the environment of different variations.Composite antidote and niblet are separated using fan,
It collects composite antidote and detects its removing toxic substances efficiency to aflatoxin B1, it is found that it still has and efficiently release aflatoxin
The effect of B1.Experiment shows that after using three times composite antidote still has the higher ability for releasing aflatoxin B1.
Rich in a large amount of fiber in buckwheat shell, and very stable structure can be kept in water, even if in the solution for a long time
Contact will not rot, and buckwheat shell has good adsorptivity.Lotus rhizome slag is as a kind of by-product without by good profit
With, but it has abundant a fibre structure and wooden structures, this structure seem after expanding treatment it is more fluffy and
Stablize.Buckwheat shell is mixed with lotus rhizome slag with certain proportion, the particle that progress extrusion processing is prepared can play potent
Adsorption capacity, the buckwheat hull component in particulate vector first embodies potent adsorption capacity, can by bacterium solution it is effective into
Divide and firmly adsorb；Lotus rhizome slag part then provides skeleton structure and expands whole contact area, can expand carrier in itself
Adsorbance, so as to promote the joint efficiency of particle in itself.This particle for having special absorption and binding performance is added to
In Methylotrophic fermentation of bacillus liquid, carrier is given full play to the secured of ingredient that effectively detoxify in microorganism and tunning
With reference to, and then that stablizes prepares a kind of composite antidote efficiently, stable, safe, and then play efficient detoxication.Buckwheat
Shell and lotus rhizome slag nutriment lack, it is impossible to meet the basic nutrition mechanism of aspergillus flavus fungi growth, buckwheat shell and lotus rhizome slag master
If fibre composition, idiotrophic substance is very barren, it is impossible to provide sufficient sugar and nutriment, institute to the growth of aspergillus flavus
The contaminated object of aspergillus flavus will not be become in itself with composite antidote.Using extrusion buckwheat shell and lotus rhizome slag to methylotrophy
Type fermentation of bacillus liquid carries out adsorption treatment, prepares aflatoxin B1 high-efficiency detoxication agent, and provides a kind of safe and efficient
The method for releasing aflatoxin B1 in corn.Puffing carrier granular is combined with the bacterial solution for having detoxication, jointly
The research for releasing aflatoxin B1 is rarely reported.
Invention content
The present invention provides a kind of method for releasing aflatoxin B1.
The Methylotrophic bacillus of the use of the present invention comes from Laboratories Accession, niblet, buckwheat shell, lotus rhizome slag
Come from farm.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of new method of degrading aflatoxin B 1, main method are that buckwheat shell and lotus rhizome slag are passed through extrusion and powder
Broken sieving prepares uniform puffing carrier granular, and carrier granular is adsorbed Methylotrophic gemma bar with certain solid-to-liquid ratio
Bacterium fermentation culture prepares a kind of composite antidote and applied to the releasing effect of aflatoxin B1 in corn.
Further, during step 8 handles wheat shell and lotus rhizome slag composite particles carrier, retained 20~200 mesh
The carrier granular of sieve.
A kind of complex carrier of degrading aflatoxin B 1 of the present invention is as follows：
(1) buckwheat shell and lotus rhizome slag are subjected to high temperature drying processing, and buckwheat shell and lotus rhizome slag is subjected to raw material and are pre-mixed place
Reason, it is characterized in that buckwheat shell and lotus rhizome slag are with 1:10~10:1 mass ratio carries out extrusion processing and prepares carrier.It and will place
It is spare to cross 20~200 mesh sieve into particle for the sample comminution managed.
(2) Methylotrophic bacillus is inoculated in seed culture medium activation, the zymotic fluid after activation is transferred later
Constant temperature incubation a couple of days in NB culture mediums.Later by the carrier granular prepared and Methylotrophic bacillus culture solution knot
It closes, the solid-to-liquid ratio of control vector particle and Methylotrophic fermentation of bacillus bacterium solution is 1:3~3:1.
During aflatoxin B1 of the composite antidote of the present invention in degrading maize, after detoxication recycling still
Has higher detoxicating activity.Composite antidote can keep more preferable stability, still have after recycling three times stronger
Detoxification ability.
Utilize National Standard Method（Number：GB/T 17480-2008）Detect the content of aflatoxin B1.
Research environment factor releases composite antidote the influence of aflatoxin B1 in mouldy corn, by mouldy jade
Rice pH is controlled, and when the pH that substrate is controlled to be mouldy niblet is 5~6, composite antidote shows efficient detoxification ability,
It is compound under equal conditions compared with the efficiency of the aflatoxin B1 in the independent degrading maize of Methylotrophic bacillus bacterium solution
The degradation capability of antidote can promote 20%~40%.
The present invention releases the application in aflatoxin B1 in corn.
The present invention is combined the buckwheat shell after extrusion and lotus rhizome slag carrier granular with solution toadstool, and then promotes solution
Malicious ability.Through report, fungi, the bacterium of many natures all show have degradation to aflatoxin B1, but for
The influence of environmental factor, external environment can change with the change of the factors such as temperature, pH, humidity, it would be generally disadvantageous to microorganism
Degrading aflatoxin B 1.The Methylotrophic bacillus of one plant of degrading aflatoxin B 1 of this laboratory screening, and to it
Metabolite has done cytotoxicity experiment, verifies it to being safe from harm property of animal.Currently there are many materials to can be used for immobilization
Material, but since a lot of reasons limits its application.Buckwheat shell and security performance of lotus rhizome slag itself are high, are handled through extrusion
Carrier granular absorption property afterwards is good.By the carrier granular that wheat shell and lotus rhizome slag are mixed with and Methylotrophic bacillus
Zymotic fluid combines, and swelling granular carrier is made fully to be combined with the removing toxic substances fungi degradation toxenzyme in zymotic fluid, is suitably promoting temperature or is changing
Become under conditions of its pH, composite antidote still maintains the activity for efficiently releasing aflatoxin B1.Utilize agricultural wastes buckwheat
Wheat husk and the puffing carrier granular absorption bacterium solution of lotus rhizome slag carry out the degradation of aflatoxin B1, are a kind of not only safety but also efficient solution
Malicious method.
The compound Methylotrophic bacillus bacterium solution of buckwheat shell and lotus rhizome slag swelling granular of the present invention, has safe efficient
The application of aflatoxin B1 in degrading maize.Composite antidote with mouldy corn is uniformly mixed, can be very good to drop
Solve the aflatoxin B1 in mouldy corn, the releasing rate of aflatoxin B1 reaches more than 70% in mouldy corn.It is sent out in effect
When mould corn pH is 5~6, under equal conditions with the aflatoxin in the independent degrading maize of Methylotrophic bacillus bacterium solution
The efficiency of B1 is compared, and is adsorbed the particulate vector releasing rate of bacterium solution and can be promoted 20%~40%.Composite antidote can keep more preferable
Stability still has stronger detoxification ability after recycling three times.
Specific embodiment
With reference to embodiment, the specific embodiment of the present invention is described in further detail.Following embodiment is to use
Come the content further illustrated the present invention, without limiting the scope of the invention.For related content according to the present invention
And the modification made, belong to the scope of the present invention.
Embodiment 1
By the buckwheat shell of purchase and lotus rhizome slag with 1:1 ratio mixes and carries out extrusion processing, the puffing material that will be prepared
Material carries out high speed pulverization process, crosses 20 mesh sieve screening swelling granular carrier, spare.
Methylotrophic bacillus is detached with scribing line on solid medium, 37 DEG C are cultivated 24 hours.By what is activated
Thalline is inoculated on the NB culture mediums of 50ml, and the Methylotrophic fermentation of bacillus liquid that constant temperature incubation 72h is obtained is used to study.
Isometric Methylotrophic bacillus bacterium solution is divided into two parts, it is multiple that portion is combined preparation with swelling granular carrier
Antidote is closed, puffing carrier is 1 with Methylotrophic fermentation of bacillus liquid solid-to-liquid ratio:3.Another is not processed.It will be in advance
Ready mouldy niblet is divided into two parts, and ready composite antidote before is mixed with the niblet that aspergillus flavus pollutes
Uniformly, while by Methylotrophic fermentation of bacillus liquid with mouldy niblet it is uniformly mixed, with the mouldy corn not processed
As a control group, after the pH for adjusting mouldy niblet is 5.5,37 DEG C of constant temperature are handled 72 hours particle, detect remaining aspergillus flavus poison
The content of plain B1.
After testing, under this control condition, Methylotrophic fermentation of bacillus liquid is to aflatoxin in mouldy corn
The releasing rate of B1 is 48%, and under identical the same terms, composite antidote is to the aflatoxin B1 releasing rate in mouldy corn
72%, it is compareed compared to Methylotrophic bacillus bacterium solution, aflatoxin B1 releasing rate improves 24%.
Embodiment 2
By the buckwheat shell of purchase and lotus rhizome slag with 4:1 ratio carries out extrusion processing, and the bulking meterial prepared is carried out
High speed pulverization process crosses 30 mesh sieve screening swelling granular carrier, spare.
Methylotrophic bacillus is detached with scribing line on solid medium, 37 DEG C are cultivated 24 hours.By what is activated
Thalline is inoculated on the NB culture mediums of 50ml, and the Methylotrophic fermentation of bacillus liquid that constant temperature incubation 72h is obtained is used to study.
Isometric Methylotrophic bacillus bacterium solution is divided into two parts, portion is combined with swelling granular carrier, puffing
Carrier is 2 with Methylotrophic fermentation of bacillus liquid solid-to-liquid ratio:1.Another is not processed.Ready mouldy jade will be shifted to an earlier date
The grain of rice is divided into two parts, and ready composite antidote before is uniformly mixed with the niblet that aspergillus flavus pollutes, while by first
Base auxotype fermentation of bacillus liquid is uniformly mixed with mouldy niblet, using the mouldy corn particle that does not process as control
Group is adjusted after the pH of mouldy niblet is 5.5, is positioned over after 37 DEG C of constant temperature handle 72 hours, is detected remaining aflatoxin B1
Content.
After testing, under this control condition, Methylotrophic fermentation of bacillus liquid is to aspergillus flavus poison in mouldy niblet
The releasing rate of plain B1 is 48%, and under identical the same terms, composite antidote is to the aflatoxin B1 releasing rate in mouldy niblet
It is 83%, is compareed compared to Methylotrophic bacillus bacterium solution, aflatoxin B1 releasing rate improves 35%.
Claims (8)
1. a kind of new method for releasing aflatoxin B1：A kind of composite antidote is prepared, it will be by the buckwheat shell of extrusion
It is formed with the compound Methylotrophic fermentation of bacillus liquid of lotus rhizome slag carrier granular.
2. carrier granular as described in claim 1 is used to adsorb Methylotrophic bacillus culture solution, it is characterized in that
The sieve that the extrusion particle is crossed is 20~200 mesh.
3. release aflatoxin B1 such as claim 1, it is characterised in that：Effect is sent out for 5~6 times composite antidotes in pH
The detoxication of mould corn is best.
4. the carrier granular of extrusion as described in claim 1, it is characterised in that buckwheat shell and lotus rhizome slag are subjected to raw material
Processing is pre-mixed, then carries out extrusion processing.
5. the carrier granular mentioned such as claim 1-4, it is characterised in that：By dried wheat shell and lotus rhizome slag with 1:10~
10:1 mass ratio carries out extrusion processing and prepares carrier granular.
6. such as composite antidote in claim 1, when detoxifying for the aflatoxin B1 in corn, can reuse
Three times.
7. a kind of new method for releasing aflatoxin B1 as described in claim 1, it is characterised in that：Carrier granular and methyl
The solid-to-liquid ratio of auxotype fermentation of bacillus liquid is 1:3~3:1.
What 8. right 1 and 2 required, the composite antidote that right 3, right 4, right 5, right 6, right 7 optimize solves in corn
Except the application in aflatoxin B1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051502.5A CN108208537A (en) | 2018-01-19 | 2018-01-19 | A kind of method for releasing aflatoxin B1 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051502.5A CN108208537A (en) | 2018-01-19 | 2018-01-19 | A kind of method for releasing aflatoxin B1 |
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| CN108208537A true CN108208537A (en) | 2018-06-29 |
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| CN201810051502.5A Pending CN108208537A (en) | 2018-01-19 | 2018-01-19 | A kind of method for releasing aflatoxin B1 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136294A (en) * | 2018-09-17 | 2019-01-04 | 河南工业大学 | A method of it releasing mycotoxin in mouldy corn and converts production xylitol |
| CN109198007A (en) * | 2018-10-29 | 2019-01-15 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of peanut storage practice for effectivelying prevent aflatoxin to infect |
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| 王燕，等: "黄曲霉毒素 B1 降解菌的分离鉴定及培养条件优化", 《河南农业科学》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136294A (en) * | 2018-09-17 | 2019-01-04 | 河南工业大学 | A method of it releasing mycotoxin in mouldy corn and converts production xylitol |
| CN109198007A (en) * | 2018-10-29 | 2019-01-15 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of peanut storage practice for effectivelying prevent aflatoxin to infect |
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