CN108208018B - Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition - Google Patents
Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition Download PDFInfo
- Publication number
- CN108208018B CN108208018B CN201711420471.8A CN201711420471A CN108208018B CN 108208018 B CN108208018 B CN 108208018B CN 201711420471 A CN201711420471 A CN 201711420471A CN 108208018 B CN108208018 B CN 108208018B
- Authority
- CN
- China
- Prior art keywords
- pesticide
- essential oil
- pine needle
- dosage
- insecticidal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide application, and particularly relates to application of Chinese pine needle leaf essential oil in an insecticidal pesticide and an insecticidal pesticide composition. The auxiliary agent component is pine needle essential oil, the preparation method is characterized in that the dosage of each L of pine needle essential oil is 0.01-2 mL, the dosage of the surfactant is 0.002-20mL, the dosage of the pesticide is 5-20% of the original dosage, the volume dosage of the ethanol is 1.7-2.5 times of the total volume dosage of the essential oil, the surfactant and the pesticide, and the balance is water. The field implementation shows that the pine needle essential oil as the pesticide adjuvant can reduce the pesticide dosage by 5-20 times and has synergistic effect on diptera, coleoptera and lepidoptera pests. Thereby having the effects of increasing the efficiency and reducing the using amount of the pesticide and having wide popularization prospect.
Description
Technical Field
The invention belongs to the field of agricultural technology application, and particularly relates to application of Chinese pine needle leaf essential oil in an insecticidal pesticide and an insecticidal pesticide composition.
Background
The insecticidal pesticide adjuvant is a general name of various auxiliary agents except active ingredients in a chemical insecticidal pesticide processing formulation, and plays an important role in the preparation of the insecticidal pesticide formulation and the endowment of the active ingredients with the best efficacy. Generally, the auxiliary agent has no biological activity, but can damage the protection of pests on the insecticidal pesticide or reduce the decomposition capability of the pests on the insecticidal pesticide, and the like to play a synergistic effect, so that the pesticide effect can be improved or prolonged, the dosage of the insecticidal pesticide can be reduced, the pesticide cost can be reduced, and the environmental damage and the insecticidal pesticide residue can be reduced.
The pesticide adjuvant in the world is developing towards high efficiency, low dosage, multifunction and low cost. The natural products such as alkaloid, glucoside, tannin, plant essential oil, etc. can be mixed with pesticides such as organochlorine, organophosphorus, organosulfur, heterocyclic, carbamate and cypermethrin, etc. to achieve synergistic effect. The essential oil is a general name of volatile oily liquid which exists in plants, can be distilled along with water vapor and has certain fragrance, is composed of compounds with small molecular weight, is volatile at normal temperature, is almost insoluble in water, is easily soluble in organic solvents with small polarity and high-concentration ethanol, is easy to decompose, and is safe to human beings and mammals at low dose. The plant essential oil can play a synergistic role by enhancing the transdermal property of the insecticidal pesticide.
The provinces such as southern Jilin, Liaoning, Hebei, Henan, Shandong, Shanxi, inner Mongolia, Shanxi, Gansu, Ningxia, Qinghai and Sichuan produced by Pinus tabuliformis Carr are one of the main tree species of the protection forest in the three North, and the total area is nearly 230 million hectares. The Chinese pine needle essential oil is mainly used for human body health care at present, and has excellent human body transdermal property.
The invention provides a Chinese pine needle essential oil as a synergistic auxiliary agent of an insecticidal pesticide and a use method thereof.
Disclosure of Invention
The invention aims to provide a botanical insecticidal pesticide auxiliary agent with a synergistic effect and a use method thereof.
The invention relates to a botanical pesticide adjuvant, which is characterized in that:
the pine needle essential oil can be used as an active synergistic auxiliary agent to be applied to the insecticidal pesticide.
The insecticidal pesticide is used for killing one or more than two pests of Coleoptera, Lepidoptera and Diptera, and the pine needle leaf essential oil has synergistic effect when being used as an active synergistic auxiliary agent for killing the pests by adopting the insecticidal pesticide.
The pesticide is one or more of deltamethrin, fenvalerate, fenpropathrin, cypermethrin, beta-cypermethrin and other pyrethroid pesticides.
The volume ratio of the pine needle essential oil to the pesticide is 0.3-1.2: 5, preferably 0.33 to 1.0: 5, more preferably 0.5 to 1.0: 5.
the use process comprises the following steps: 1) mixing the essential oil of the pine needle with a surfactant; 2) adding an insecticidal pesticide; 3) dissolving with ethanol; 4) adding water to dilute to 5-20 times of the original pesticide (before adding pine needle essential oil) for field spraying;
the volume ratio of the pine needle essential oil to the surfactant is 1:0.1-1:20, preferably 1:0.2-1: 20; the volume ratio of the pine needle essential oil to the pesticide is 0.3-1.2: 5, preferably 0.33 to 1.0: 5, more preferably 0.5 to 1.0: 5; the volume dosage of the ethanol is 1.5-20 times, preferably 1.7-2.5 times of the total volume dosage of the essential oil, the surfactant and the pesticide.
In the field spraying process, the dosage of the insecticidal pesticide added with the pine needle essential oil is 5 to 20 percent of the dosage of the insecticidal pesticide before the pine needle essential oil is not added.
The surfactant is a detergent.
An insecticidal pesticide composition comprises Chinese pine needle essential oil and an insecticidal pesticide.
The insecticidal pesticide is used for killing one or more than two pests in Coleoptera, Lepidoptera and Diptera, and the pine needle leaf essential oil has synergistic effect when being used as an active synergistic auxiliary agent for killing the pests by the insecticidal pesticide;
the volume ratio of the pine needle essential oil to the pesticide is 0.3-1.2: 5, preferably 0.33 to 1.0: 5, more preferably 0.5 to 1.0: 5.
the pesticide is one or more of deltamethrin, fenvalerate, fenpropathrin, cypermethrin, beta-cypermethrin and other pyrethroid pesticides.
The method for extracting the pine needle essential oil comprises the following steps: cutting the pine needle of the Chinese pine to about 1cm (0.6-1.5 cm), putting 100-200g (150 g) of the pine needle into a round-bottom flask, adding 2-4 times (3 times) of water by mass, connecting a volatile oil extractor at the upper opening of the round-bottom flask, and heating for 2-5h (4 h) at 100-130 ℃ (115 ℃ in the case) after the installation to obtain the essential oil of the pine needle of the Chinese pine.
The field implementation shows that the pine needle essential oil as the pesticide adjuvant can reduce the pesticide dosage by 5-20 times and has synergistic effect on diptera, coleoptera and lepidoptera pests. Thereby having the effects of increasing the efficiency and reducing the using amount of the pesticide and having wide popularization prospect. The insecticidal pesticide auxiliary agent reduces the dosage of the insecticidal pesticide by 5-20 times, has better effect, and has the effects of improving the effect and reducing the dosage of the pesticide by adopting the insecticidal pesticide auxiliary agent.
Detailed Description
Test of control effect of different pine needle essential oils
Example one
This example is divided into 3 test groups and 1 control group, and each group is close to each other, and every group sprays the area and is 2.5 mu. The insecticidal pesticide is cypermethrin (the content of cypermethrin is more than or equal to 10 percent), and the spraying object is corylus heterophylla bush damaged by corylus heterophylla gall midge;
the preparation method of the high-dose group (ZYW-Y-1) comprises the following steps: 1mL of Chinese pine needle essential oil, 1.2mL of liquid detergent (surfactant as emulsifier) and 5mL of cypermethrin, and the components are mixed, dissolved in 20mL of ethanol and diluted to 5L by adding water.
The preparation method of the medium dose group (ZYW-Y-2) comprises the following steps: 0.5mL of Chinese pine needle essential oil, 0.6mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the mixture is dissolved in 20mL of ethanol and diluted to 5L by adding water.
Preparation method of low-dose group (ZYW-Y-3): 0.33mL of Chinese pine needle essential oil, 0.4mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the mixture is dissolved in 20mL of ethanol and diluted to 5L by adding water.
Preparation method of control group (ck): 50mL of cypermethrin is diluted to 5L by adding water.
The spraying time and the method are as follows: spraying the whole plant of hazel gall midge in the damage period in 1 day of 5 months.
The investigation and statistical method comprises the following steps: at 30 days after 5 months, more than 1000 branches are randomly sampled to observe the damage of leaves and fruits caused by corylus heterophylla gall midge, and SPSS 20.0 is statistically different and significant.
Effects of the implementation
TABLE 1 synergistic effect of needle leaf essential oil of Chinese pine for reducing drug and preventing Hazelnut gall midge
Indicates a very significant difference compared to the control group (p <0.01)
Example two
This embodiment is divided into 3 test groups and 1 control group, and each group is close to each other, and every group sprays the area and is 2.5 mu, and with embodiment one in same test field, close to each other. The insecticidal pesticide is cypermethrin (the content of cypermethrin is more than or equal to 10 percent), and the spraying object is corylus heterophylla bush damaged by corylus heterophylla gall midge;
the preparation method of the high-dose group (ZYW-H-1) comprises the following steps: 1mL of Korean pine needle essential oil, 1.2mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the components are mixed, dissolved in ethanol to 20mL and diluted to 5L by adding water.
The preparation method of the medium dose group (ZYW-H-2) comprises the following steps: 0.5mL of Korean pine needle essential oil, 0.6mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the components are mixed, dissolved in ethanol to 20mL and diluted to 5L by adding water.
Preparation method of low dose group (ZYW-H-3): 0.33mL of Chinese pine needle essential oil, 0.4mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the mixture is dissolved in 20mL of ethanol and diluted to 5L by adding water.
Preparation method of control group (ck): 50mL of cypermethrin is diluted to 5L by adding water.
The spraying time and the method are as follows: spraying the whole plant of hazel gall midge in the damage period in 1 day of 5 months.
The investigation and statistical method comprises the following steps: at 30 days after 5 months, more than 1000 branches are randomly sampled to observe the damage of leaves and fruits caused by corylus heterophylla gall midge, and SPSS 20.0 is statistically different and significant. Effects of the implementation
TABLE 2 synergistic effect of pinus koraiensis needle essential oil in reducing drug and preventing hazel gall midge
EXAMPLE III
This example is divided into 3 test groups and 1 control group, each group is close to each other, and every group sprays the area and is 2.5 mu, and with example one, two belong to a slice of test field, close to each other). The insecticidal pesticide is cypermethrin (the content of cypermethrin is more than or equal to 10 percent), and the spraying object is corylus heterophylla bush damaged by corylus heterophylla gall midge;
preparation method of high dose group (ZYW-Z-1): 1mL of pinus sylvestris needle essential oil, 1.2mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and the liquid detergent are mixed, dissolved in ethanol to 20mL and diluted to 5L by adding water.
The preparation method of the medium dose group (ZYW-Z-2) comprises the following steps: 0.5mL of camphor pine needle essential oil, 0.6mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and after mixing, adding ethanol to dissolve the mixture to 20mL, and adding water to dilute the mixture to 5L.
Preparation method of low dose group (ZYW-Z-3): 0.33mL of camphor pine needle essential oil, 0.4mL of liquid detergent (emulsifier) and 5mL of cypermethrin, and after mixing, adding ethanol to dissolve the mixture to 20mL, and adding water to dilute the mixture to 5L.
Preparation method of control group (ck): 50mL of beta-cypermethrin is diluted to 5L by adding water.
The spraying time and the method are as follows: spraying the whole plant of hazel gall midge in the damage period in 1 day of 5 months.
The investigation and statistical method comprises the following steps: at 30 days after 5 months, more than 1000 branches are randomly sampled to observe the damage of leaves and fruits caused by corylus heterophylla gall midge, and SPSS 20.0 is statistically different and significant. Effects of the implementation
TABLE 1 synergistic effect of essential oil of Pinus sylvestris needle for reducing drug and preventing Hazel gall midge
From examples 1 to 3, it can be concluded that:
whether the hazel gall midge pest control synergistic effect is achieved by the pine needle essential oil of the Chinese pine, the pine needle essential oil of the Korean pine or the pine needle essential oil of the pinus sylvestris; the prevention and control synergistic effect of the pine needle essential oil of the Chinese pine is the best, and the protection effect of the pine needle essential oil of the Chinese pine on leaves and fruits is better than that of the pine needle essential oil of the Korean pine and the pine needle essential oil of the pinus sylvestris.
Test of controlling Effect of different pests
Example four
The pest aimed at by the embodiment is hazelnut weevil of coleoptera, the pesticide is cypermethrin (the content of cypermethrin is more than or equal to 10 percent), and the spraying object is a hazel bush damaged by the hazelnut weevil; this example was divided into three test groups and a control group, each group being adjacent to each other, and the spray area of each group was 2.5 hectares.
High dose group (ZSX-1) formulation method: 1mL of pine needle essential oil, 1.2mL of liquid detergent and 5mL of cypermethrin, mixing, adding ethanol to dissolve the mixture to 20mL, adding water to dilute the mixture to 5L, and spraying the mixture in a field;
preparation method of medium dose group (ZSX-2): 0.5mL of pine needle essential oil, 0.6mL of liquid detergent and 5mL of cypermethrin, mixing, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in the field;
formulation method of low dose group (ZSX-3): 0.33mL of essential oil, 0.4mL of liquid detergent and 5mL of cypermethrin, mixing, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in the field
Control group: 50mL of cypermethrin, diluting to 5L with water, and spraying in field
The spraying time and the method are as follows: spraying the whole plant in hazelnut weevil hazard period for 5 months and 30 days
The investigation and statistical method comprises the following steps: 7, 15 days, randomly sampling more than 1000 branches to observe the damage condition of the hazelnut weevil to the fruits, and showing the statistical difference of SPSS 20.0
TABLE 2 synergistic and drug-reducing test of Chinese pine essential oil-hazelnut weevil hazard rate survey
| Test set | Plant-produced hazelnut | Weight of wormhole | Percentage of worm fruits% | Weight of diseased fruit | The rate of fruit diseases% |
| ZSX-1 | 885.31 | 0 | 0** | 7.25 | 0.81** |
| ZSX-2 | 779.97 | 3.1 | 0.46** | 9.35 | 1.20** |
| ZSX-3 | 620 | 4.0 | 0.56** | 10.28 | 1.66** |
| CK | 450 | 11 | 2.44** | 11.55 | 2.56** |
Indicates a very significant difference compared to the control group (p <0.01)
EXAMPLE five
The pests aimed at by the embodiment are leaf-rolling weevils of coleoptera, the used pesticide is deltamethrin (the content of active ingredients is more than or equal to 2.5 percent), and the spraying object is corylus heterophylla shrubs and bushes harmed by the leaf-rolling weevils
This example was divided into three test groups and a control group, each group being adjacent to each other, and the spray area of each group was 2.5 hectares.
Preparation method of high dose group (JYX-1): 1mL of pine needle essential oil, 1.2mL of liquid detergent and 5mL of deltamethrin, mixing, adding ethanol to dissolve the mixture to 20mL, adding water to dilute the mixture to 5L, and spraying the mixture in a field;
preparation method of medium dose group (JYX-2): 0.5mL of pine needle essential oil, 0.6mL of liquid detergent and 5mL of deltamethrin insecticide are mixed, then ethanol is added to dissolve the mixture to 20mL, water is added to dilute the mixture to 5L, and the mixture is sprayed in the field;
preparation method of low dose group (JYX-3): 0.33mL of essential oil, 0.4mL of liquid detergent and 5mL of deltamethrin, mixing, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in field
Control group: 50mL of deltamethrin, diluting to 5L with water, and spraying in field
The spraying time and the method are as follows: spraying the whole plant in 6 months and 1 day in the damage period of leaf-rolling weevil
The investigation and statistical method comprises the following steps: 7, 15 days, randomly sampling more than 1000 branches to observe the damage condition of leaf-rolling weevils to hazelnut leaves, and showing the statistical difference significance of SPSS 20.0
TABLE 3 synergistic and drug reduction test of Chinese pine essential oil-investigation of the hazard rate of weevil leaf roll
| Test set | Mean number of insect leaves | Number of samples | The ratio of insect leaves% |
| JYX-1 | 10 | 1989 | 0.50** |
| JYX-2 | 18 | 2011 | 0.90** |
| JYX-3 | 23 | 1879 | 1.22** |
| CK | 95 | 1996 | 4.76** |
Indicates a very significant difference compared to the control group (p <0.01)
EXAMPLE six
The pests aimed at in the embodiment are Mongolian weevils of Coleoptera, the used pesticide is deltamethrin (the content of beta-cypermethrin is more than or equal to 2.5 percent), and the spraying object is a corn plant damaged by the Mongolian weevils
This example was divided into three test groups and a control group, each group being adjacent to each other, and the spray area of each group was 2.5 hectares.
Preparation method of high dose group (MGX-1): 1mL of pine needle essential oil, 1.2mL of liquid detergent and 5mL of deltamethrin, mixing, adding ethanol to dissolve the mixture to 20mL, adding water to dilute the mixture to 5L, and spraying the mixture in a field;
the preparation method of the medium dose group (MGX-2) comprises the following steps: 0.5mL of pine needle essential oil, 0.6mL of liquid detergent and 5mL of deltamethrin insecticide are mixed, then ethanol is added to dissolve the mixture to 20mL, water is added to dilute the mixture to 5L, and the mixture is sprayed in the field;
preparation method of low dose group (MGX-3): 0.33mL of essential oil, 0.4mL of liquid detergent and 5mL of deltamethrin, mixing, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in field
Control group: 50mL of deltamethrin, diluting to 5L with water, and spraying in field
The spraying time and the method are as follows: spraying the whole plant in the endangered period of Mongolian weevil in 1 day in 6 months
The investigation and statistical method comprises the following steps: 7, 15 days, randomly sampling more than 1000 branches to observe the damage condition of the corn leaf caused by the mongolian weevil, and showing the statistical difference of SPSS 20.0
TABLE 4 synergistic and drug-reducing test of Chinese pine essential oil-investigation of Mongolian weevil hazard rate
| Test set | Mean number of insect leaves | Number of samples | The ratio of insect leaves% |
| MGX-1 | 20 | 2000 | 1.00** |
| MGX-2 | 35 | 2010 | 1.74** |
| MGX-3 | 55 | 1980 | 2.78** |
| Ck | 108 | 2030 | 5.32** |
Indicates a very significant difference compared to the control group (p <0.01)
EXAMPLE seven
The pests aimed at by the embodiment are Lepidoptera anomala, the used pesticide is beta-cypermethrin (the content of active ingredients is more than or equal to 10 percent), and the spraying object is a corn plant damaged by the anomala
This example was divided into three test groups and a control group, each group being adjacent to each other, and the spray area of each group was 2.5 hectares.
Preparation method of high dose group (MGX-1): 1mL of pine needle essential oil, 1.2mL of liquid detergent and 5mL of beta-cypermethrin, mixing, adding ethanol to dissolve the mixture to 20mL, adding water to dilute the mixture to 5L, and spraying the mixture in a field;
the preparation method of the medium dose group (MGX-2) comprises the following steps: 0.5mL of pine needle essential oil, 0.6mL of liquid detergent and 5mL of efficient cypermethrin insecticide are mixed, then ethanol is added to dissolve the mixture to 20mL, water is added to dilute the mixture to 5L, and the mixture is sprayed in the field;
preparation method of low dose group (MGX-3): mixing pine needle essential oil 0.33mL, detergent 0.4mL and beta-cypermethrin 5mL, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in field
Control group: 50mL of beta-cypermethrin, diluting to 5L with water, and spraying in field
The spraying time and the method are as follows: spraying the whole plant of the Onychostoma longirostrum in the damage period of 1 day in 6 months
The investigation and statistical method comprises the following steps: 7, 15 days, randomly sampling more than 1000 branches to observe the damage condition of apple tree leaves caused by tenebrio molitor, wherein the statistical difference significance of SPSS 20.0
TABLE 5 synergistic and drug reduction test of Chinese pine essential oil-investigation of Haematococcus hazard rate
| Test set | Mean number of insect leaves | Number of samples | The ratio of insect leaves% |
| TMM-1 | 5 | 1000 | 0.50** |
| TMM-2 | 10 | 1010 | 1.00** |
| TMM-3 | 13 | 1080 | 1.31** |
| Ck | 98 | 1130 | 8.67** |
Indicates a very significant difference compared to the control group (p <0.01)
Example eight
The pests aimed at in the embodiment are lepidoptera fin populi navicularis moths, the used pesticide is beta-cypermethrin (the content of active ingredients is more than or equal to 10 percent), the spraying object is poplar leaves damaged by the populi navicularis moths, the embodiment is divided into three testing groups and a control group, the groups are close to each other, and the spraying area of each group is 2.5 hectares.
Preparation method of high dose group (MGX-1): 1mL of pine needle essential oil, 1.2mL of liquid detergent and 5mL of beta-cypermethrin, mixing, adding ethanol to dissolve the mixture to 20mL, adding water to dilute the mixture to 5L, and spraying the mixture in a forest;
the preparation method of the medium dose group (MGX-2) comprises the following steps: 0.5mL of pine needle essential oil, 0.6mL of liquid detergent and 5mL of efficient cypermethrin insecticide are mixed, then ethanol is added to dissolve the mixture to 20mL, water is added to dilute the mixture to 5L, and the mixture is sprayed in forests;
preparation method of low dose group (MGX-3): 0.33mL of essential oil, 0.4mL of liquid detergent and 5mL of beta-cypermethrin, mixing, adding ethanol to dissolve to 20mL, adding water to dilute to 5L, and spraying in forest
Control group: 50mL of beta-cypermethrin, diluting to 5L with water, and spraying in forest
The spraying time and the method are as follows: spraying the whole plant of Bombycis Mori in 6 months and 1 day
The investigation and statistical method comprises the following steps: 7, 15 days, randomly sampling more than 1000 branches to observe the condition that the blade of the poplar is damaged by the poplars navicularia moths, wherein the statistical difference of SPSS 20.0 has significance
TABLE 6 synergistic and drug-reducing test of Chinese pine essential oil-investigation of harm rate of Bombycis mori
| Test set | Mean number of insect leaves | Number of samples | The ratio of insect leaves% |
| YSZ-1 | 53 | 5000 | 1.06** |
| YSZ-2 | 70 | 4910 | 1.43** |
| YSZ-3 | 95 | 5080 | 1.87** |
| Ck | 192 | 5400 | 3.56** |
Indicates a very significant difference compared to the control group (p <0.01)
The results of the first, the second, the third, the fourth, the fifth, the sixth, the seventh and the eighth examples show that when the pine needle leaf essential oil is used as an insecticidal auxiliary agent, the insecticidal pesticide is 5m, the dosage of the essential oil is 0.33 to 1.0mL, and the dosage of the pyrethroid insecticidal pesticide is 1/10 of a control group, the experiments of reducing the pesticide by the essential oil of hazel gall midge mosquito of diptera, poplarch navicula, tenebrio molitor, hazelnut weevil of coleoptera, mongolian weevil and leaf-curl weevil are carried out. The damage rate of the experimental group to pests is obviously lower than that of the control group, and the damage rate is gradually reduced along with the increase of the concentration of the essential oil.
Experiment of insecticidal effect:
experiments on insecticidal effect by taking high-dose group insecticidal pesticide as example
Specific experiments are as follows: on days 17-18 at 6 months, 100 insects of the beetles, the poplars, the hazel gall midge, the hazel nut weevil, the leaf rolling weevil and the Mongolian weevil are caught from the field, each insect is divided into two parts, 50 parts are placed in plastic pots, and the same amount of plant leaves are placed in each plastic pot. Preparing high-dose group insecticidal pesticide corresponding to pests and conventional pesticide, uniformly spraying the high-dose group insecticidal pesticide and the conventional pesticide in a plastic basin, turning over plant leaves during spraying to fully apply the pesticide, covering and sealing the plastic basin with gauze, and counting the mortality of the pests 24h and 48h after spraying. Each set of experiments was repeated three times with the following results:
TABLE 7 poisoning mortality rates of the pesticidal pesticide of the present invention and the conventional pesticidal pesticide on pests
The insecticidal effect experiment shows that: 1. the insecticidal pesticide prepared by the auxiliary agent has good poisoning effect on diptera, coleoptera and lepidoptera pests, and the best hazelnut weevil and hazelnut gall midge can reach 97-98%; 2. the insecticidal pesticide prepared by the aid has far higher poisoning effect on 6 pests than that of the conventional insecticidal pesticide. The adjuvant enhances the killing effect of the insecticidal pesticide on the basis of ensuring that the pesticide dosage is 1/10, can act on diptera, lepidoptera and coleoptera pests, and is an attenuated and synergistic insecticidal pesticide adjuvant with good effect.
Claims (8)
1. The application of the pine needle essential oil in the pesticide is characterized in that: the pine needle essential oil can be used as an active synergistic auxiliary agent to be applied to insecticidal pesticides; 1) mixing the essential oil of the pine needle with a surfactant; 2) adding an insecticidal pesticide; 3) dissolving with ethanol; 4) adding water to dilute to 5-20 times of the original pesticide for field spraying, wherein the original pesticide refers to pesticide before adding pine needle essential oil;
the volume ratio of the pine needle essential oil to the surfactant is 1:0.1-1: 20; the volume ratio of the pine needle essential oil to the pesticide is 0.3-1.2: 5; the volume dosage of the ethanol is 1.5 to 20 times of the total volume dosage of the essential oil, the surfactant and the pesticide;
the pesticide is one or more than two of deltamethrin, fenvalerate, fenpropathrin, cypermethrin and beta-cypermethrin.
2. Use according to claim 1, characterized in that: the volume ratio of the pine needle essential oil to the surfactant is 1:0.2-1:20 times; the volume ratio of the pine needle essential oil to the pesticide is 0.33-1.0: 5; the volume dosage of the ethanol is 1.7-2.5 times of the total volume dosage of the essential oil, the surfactant and the pesticide.
3. Use according to claim 1 or 2, characterized in that: the volume ratio of the pine needle essential oil to the pesticide is 0.5-1.0: 5.
4. use according to claim 1, characterized in that: in the field spraying process, the dosage of the insecticidal pesticide added with the pine needle essential oil is 5 to 20 percent of the dosage of the insecticidal pesticide before the pine needle essential oil is not added.
5. Use according to claim 1, characterized in that: the surfactant is a detergent.
6. An insecticidal pesticide composition, which is characterized in that: the pesticide composition comprises pine needle essential oil and a pesticide, wherein the pine needle essential oil can be used as an active synergistic auxiliary agent to be applied to the pesticide; 1) mixing the essential oil of the pine needle with a surfactant; 2) adding an insecticidal pesticide; 3) dissolving with ethanol; 4) adding water to dilute to 5-20 times of the original pesticide for field spraying, wherein the original pesticide refers to pesticide before adding pine needle essential oil;
the volume ratio of the pine needle essential oil to the surfactant is 1:0.1-1: 20; the volume ratio of the pine needle essential oil to the pesticide is 0.3-1.2: 5; the volume dosage of the ethanol is 1.5 to 20 times of the total volume dosage of the essential oil, the surfactant and the pesticide;
the pesticide is one or more than two of deltamethrin, fenvalerate, fenpropathrin, cypermethrin and beta-cypermethrin.
7. The pesticidal composition according to claim 6, characterized in that: the volume ratio of the pine needle essential oil to the surfactant is 1:0.2-1:20 times; the volume ratio of the pine needle essential oil to the pesticide is 0.33-1.0: 5; the volume dosage of the ethanol is 1.7-2.5 times of the total volume dosage of the essential oil, the surfactant and the pesticide.
8. The pesticidal composition according to claim 6 or 7, characterized in that: the volume ratio of the pine needle essential oil to the pesticide is 0.5-1.0: 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711420471.8A CN108208018B (en) | 2017-12-25 | 2017-12-25 | Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711420471.8A CN108208018B (en) | 2017-12-25 | 2017-12-25 | Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108208018A CN108208018A (en) | 2018-06-29 |
| CN108208018B true CN108208018B (en) | 2020-10-16 |
Family
ID=62648845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711420471.8A Active CN108208018B (en) | 2017-12-25 | 2017-12-25 | Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108208018B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089530A (en) * | 2018-07-31 | 2019-08-06 | 吉林大学 | A kind of essential oil composition and preparation method thereof with insecticidal function |
| CN114195581B (en) * | 2021-12-21 | 2023-03-24 | 岫岩满族自治县禾谷现代农业发展有限公司 | Edible fungus nutrition synergistic additive and preparation method and application thereof |
| FR3130510A1 (en) * | 2021-12-22 | 2023-06-23 | Berkem Developpement | Use of procyanidolic oligomers as an activator for pesticide products |
| CN117426399B (en) * | 2023-12-18 | 2024-03-08 | 海南弘德农林技术有限公司 | Plant-source pesticide fertilizer synergist and preparation method and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0945066A1 (en) * | 1998-03-26 | 1999-09-29 | Safergro Laboratories, Inc. | Natural pesticide containing garlic |
| CN1305723A (en) * | 2001-01-21 | 2001-08-01 | 西北农林科技大学无公害农药研究服务中心 | Attractant for coleoptera pests of needle-leave forest and its preparing process |
| CN1322110A (en) * | 1998-07-28 | 2001-11-14 | 艾科斯迈特技术公司 | Synergistic and residual pesticidal compositions contaiing plant essential oils |
| CN101606517A (en) * | 2009-07-27 | 2009-12-23 | 深圳诺普信农化股份有限公司 | A kind of farm chemical emulsion preparation |
| CN101971800A (en) * | 2010-06-11 | 2011-02-16 | 深圳诺普信农化股份有限公司 | Combined solvent and application thereof |
| CN102283254A (en) * | 2010-03-17 | 2011-12-21 | 钟韬 | Plant source environmentally-friendly pesticide preparation |
| WO2013148329A1 (en) * | 2012-03-26 | 2013-10-03 | Pronutria, Inc. | Charged nutritive proteins and methods |
| CN103651584A (en) * | 2013-11-30 | 2014-03-26 | 房伟 | Insecticide taking pine leaves as raw material |
| JP2014201581A (en) * | 2013-04-10 | 2014-10-27 | 株式会社大阪製薬 | Sanitary insect pest repellent |
| CN104757038A (en) * | 2015-03-23 | 2015-07-08 | 苏州谷力生物科技有限公司 | Micro-emulsified culicifuge and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060165746A1 (en) * | 2005-01-24 | 2006-07-27 | Arie Markus | Formulations containing microencapsulated essential oils |
| US20070098750A1 (en) * | 2005-09-01 | 2007-05-03 | Ecosmart Technologies, Inc. | Pesticidal compositions containing isopropyl myristate and analogs of same as a synergist for plant essential oils |
-
2017
- 2017-12-25 CN CN201711420471.8A patent/CN108208018B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0945066A1 (en) * | 1998-03-26 | 1999-09-29 | Safergro Laboratories, Inc. | Natural pesticide containing garlic |
| CN1322110A (en) * | 1998-07-28 | 2001-11-14 | 艾科斯迈特技术公司 | Synergistic and residual pesticidal compositions contaiing plant essential oils |
| CN1305723A (en) * | 2001-01-21 | 2001-08-01 | 西北农林科技大学无公害农药研究服务中心 | Attractant for coleoptera pests of needle-leave forest and its preparing process |
| CN101606517A (en) * | 2009-07-27 | 2009-12-23 | 深圳诺普信农化股份有限公司 | A kind of farm chemical emulsion preparation |
| CN102283254A (en) * | 2010-03-17 | 2011-12-21 | 钟韬 | Plant source environmentally-friendly pesticide preparation |
| CN101971800A (en) * | 2010-06-11 | 2011-02-16 | 深圳诺普信农化股份有限公司 | Combined solvent and application thereof |
| WO2013148329A1 (en) * | 2012-03-26 | 2013-10-03 | Pronutria, Inc. | Charged nutritive proteins and methods |
| JP2014201581A (en) * | 2013-04-10 | 2014-10-27 | 株式会社大阪製薬 | Sanitary insect pest repellent |
| CN103651584A (en) * | 2013-11-30 | 2014-03-26 | 房伟 | Insecticide taking pine leaves as raw material |
| CN104757038A (en) * | 2015-03-23 | 2015-07-08 | 苏州谷力生物科技有限公司 | Micro-emulsified culicifuge and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| Chemical Composition and Antioxidant Activity of Essential Oil of Six Pinus Taxa Native to China;Xie Q;《Molecules》;20151231;第20卷(第5期);全文 * |
| 五种植物精油对氟虫睛的增效与渗透促进作用;吴玮;《华东昆虫学报》;20081231;第17卷(第4期);全文 * |
| 油松针叶精油中的单萜成分;吴京科;《北京林业大学学报》;19941031;第16卷(第4期);第16页 * |
| 臭柏、油松精油的提取与抑菌活性;郭秀艳;《中国博士学位论文全文数据库-农业科技辑》;20090930;第23-26页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108208018A (en) | 2018-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108208018B (en) | Application of Chinese pine needle essential oil in insecticidal pesticide and insecticidal pesticide composition | |
| Weinzierl | Botanical insecticides, soaps, and oils | |
| KR102094916B1 (en) | Insecticide composition for Dermanyssus gallinae, and the preparation method thereof | |
| CN101223892B (en) | Toosendanin-containing compound pesticide and preparing method thereof | |
| US20070237837A1 (en) | Biocompatible tea tree oil compositions | |
| US20130125451A1 (en) | Method for repelling and/or controlling pests | |
| KR101270628B1 (en) | The composition comprising extraction or fraction of Phellodendron amurense having insect resistance or insecticidal activities for protection of cultural heritage | |
| KR20190052979A (en) | Organic agricultural material composition for pest management by using 4 kinds of plant extracts | |
| CN105145663B (en) | A kind of plant pesticide composition containing nimbin and eucalyptus oil | |
| KR20130114537A (en) | Due to the complex at the plant pest control for natural composition and manufacturing method | |
| KR101270630B1 (en) | The composition comprising the extraction or fraction of oak vinegar having insect resistance or insecticidal activities for protection of cultural heritage | |
| US20050084545A1 (en) | Non phytotoxic biocide composition containing tea tree oil and method of production the same | |
| US6505436B2 (en) | Capsicum based pesticide and method of use | |
| KR101778051B1 (en) | Composition for controlling mosquito comprising phytoecdysteroid or plant extract containing phytoecdysteroid as active ingredient and uses thereof | |
| Mouna et al. | Insecticidal effect of two aqueous extracts from the leaves of Salvia officinalis and Eucalyptus camaldulensis against Aphis fabae | |
| US20230276795A1 (en) | Termite repellant and wood protector | |
| AU2011210381A1 (en) | A botanical pesticide for agriculture/horticulture crops | |
| US20190008163A1 (en) | Animal repellant | |
| US20040128908A1 (en) | Capsicum based seed coating and method of use | |
| Singh et al. | Mosquito repellent and oviposition deterrent activities of Laggera aurita plant extract against malaria vector Anopheles stephensi. | |
| KR20130026742A (en) | The preparations method of mosquito and harmful insect eradication such as a natural insecticide extracted from the ginkgo tree ingredient | |
| CN103918705B (en) | Pesticidal combination containing spiral shell worm ethyl ester and gamma cyhalothrin and application thereof | |
| Noveriza et al. | Effectiveness of several dosage formula of oil and nano emulsion of citronella against vascular streak dieback (VSD) disease on cocoa | |
| Lina et al. | Safety and effectiveness of mixed plant extracts formulation against cabbages pests under field conditions | |
| CN1799360A (en) | A group of soluble liquid pesticide making carbosulfan as raw material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |