CN108192851A - Dongfanghong bacteria strain, Dongfanghong bacteria agent and its application - Google Patents
Dongfanghong bacteria strain, Dongfanghong bacteria agent and its application Download PDFInfo
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- CN108192851A CN108192851A CN201810202487.XA CN201810202487A CN108192851A CN 108192851 A CN108192851 A CN 108192851A CN 201810202487 A CN201810202487 A CN 201810202487A CN 108192851 A CN108192851 A CN 108192851A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 81
- 241001272684 Xanthomonas campestris pv. oryzae Species 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 238000011218 seed culture Methods 0.000 claims description 10
- 239000012681 biocontrol agent Substances 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000011534 incubation Methods 0.000 abstract description 2
- 238000011017 operating method Methods 0.000 abstract description 2
- 231100000572 poisoning Toxicity 0.000 abstract description 2
- 230000000607 poisoning effect Effects 0.000 abstract description 2
- 230000000243 photosynthetic effect Effects 0.000 description 16
- 239000003795 chemical substances by application Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 11
- 241000209094 Oryza Species 0.000 description 10
- 235000007164 Oryza sativa Nutrition 0.000 description 10
- 235000009566 rice Nutrition 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000000575 pesticide Substances 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 244000170916 Paeonia officinalis Species 0.000 description 1
- 235000006484 Paeonia officinalis Nutrition 0.000 description 1
- 241001478322 Rhodobium orientis Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of Dongfanghong bacteria strain, and the Dongfanghong bacteria strain is Dongfanghong bacterium I 11, is preserved in China typical culture collection center, and deposit number is CCTCC NO:M2012368, compared with prior art, the simple production process of Dongfanghong bacterial strain, incubation time are short, and biocontrol agent can be prepared by simple, quick, inexpensive operating method in the bacterial strain, bacterial blight of rice can effectively be prevented, and have the characteristics that it is environmentally friendly, using it is simple and convenient, at low cost, pollution-free, to crop without poisoning.
Description
Technical field
The present invention relates to biological pesticide technical fields, and in particular to a kind of new microbe agent (Dongfanghong bacteria agent)
Application in prevention and control bacterial blight of rice.
Background technology
One of bacterial leaf-blight, rice main bacteria venereal disease evil, by rice Xanthomonas (Xanthomonas oryzae
Pv.oryzae it) infects and causes, each rice region has generation in China.After blade is by pathogen infection, at the beginning of blade tip and edge
Raw yellow green spot, after along vein development sickly, the long streak of yellowish-brown, finally become canescence and withered.Diseased plant easily falls
Volt, spike of rice is unreal, big to yield effect, once the underproduction occurs up to 10%-20%, up to 50%-60%, serious prestige when serious
Coerce the safety in production of rice.
At present, the prevention of bacterial blight of rice depends on resistant variety selection and breeding and using chemical pesticide, big to quantify
Learn to farm medicine long-time service lead to problems such as pesticide residue, Disease Resistance increase, environmental pollution, seriously affect agricultural it is sustainable
Development.And resistance breeding difficulty is big, the period is long, corresponding agricultural measures are complicated, economic input is big.In recent years, biological control with
Its eco-friendly, at low cost, free of contamination feature become control of plant disease popular research field and it is current and
The development trend of following control of plant disease.
Some researches show that include azophenlyene class compound in the metabolite of molten bacillus to significantly inhibit leaf spot bacteria
Activity, molten bacillus have good effect in crop field to bacterial leaf-blight.Bacillus can also inhibit the activity of leaf spot bacteria,
Raw test shows to have sprayed on the rice leaf of bacillus, and scab length significantly reduces.This is a series of research shows that water
The biological control of the bacterial blight of rice has broad application prospects.Photosynthetic bacteria (Photosynthetic Bacteria, PSB)
It is the general name for the photosynthetic a kind of bacterium that can not put oxygen under anaerobic, research finds that photosynthetic bacteria can swash
The immune response of host living, inhibits the growth of pathogen, has extraordinary effect in vegetable disease prevention, and in rice
It is not had been reported that also in terms of the prevention and control of bacterial leaf-blight, the present invention is for the first time using the Dongfanghong bacteria agent in photosynthetic bacteria big
Field carries out prevention and control application to bacterial blight of rice, the results showed that control effect protrudes, and biocontrol microorganisms have wide promotional value.
Invention content
In view of the deficienciess of the prior art, it is an object of the present invention to provide a kind of Dongfanghong bacteria strain, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve these goals, the present invention is to realize by the following technical solutions：A kind of Dongfanghong bacteria strain,
The Dongfanghong bacteria strain is Dongfanghong bacterium I-1-1, is preserved in China typical culture collection center, deposit number CCTCC
NO:M2012368。
Dongfanghong bacteria agent containing the bacterial strain.
Further, the Dongfanghong bacteria agent is by Dongfanghong bacteria strain is activated, prepared by seed culture, productive culture
It obtains.
Further, bacteria concentration 108-1010cfu/mL。
Application of the Dongfanghong bacteria agent in prevention bacterial blight of rice.
Beneficial effects of the present invention：A kind of Dongfanghong bacteria strain of the present invention, the simple production process of Dongfanghong bacterial strain,
Incubation time is short, and biocontrol agent can be prepared by simple, quick, inexpensive operating method in the bacterial strain, can effectively prevent
Harness the river the bacterial blight of rice, and with it is environmentally friendly, using it is simple and convenient, at low cost, pollution-free, to crop without spies such as poisoning
Point.
Description of the drawings
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon：
Fig. 1 is for Dongfanghong bacteria agent in crop field to the control effect statistical chart of bacterial blight of rice；
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
The present invention provides a kind of technical solution：A kind of Dongfanghong bacteria strain, the Dongfanghong bacteria strain are Dongfanghong bacterium I-
1-1, is preserved in China typical culture collection center, and deposit number is CCTCC NO: M2012368.
As one embodiment of the present of invention：The Dongfanghong bacteria strain is Dongfanghong bacterium I-1-1 (Rhodobium
Orientis I-1-1), China typical culture collection center is preserved in, deposit number is CCTCC NO:M2012368.It is main
Biological property is wanted to have：4d~8d is cultivated on bilayer solid plating medium, forms red circular colonies, colony edge is whole
Neat smooth, colony diameter 0.2mm~0.60mm；It is in peony that 7d is cultivated in fluid nutrient medium；It is raw under anaerobism and micro-oxygen conditions
It is long good；Physiology characteristic for V-P reactions are negative with clark and Lubsreaction, H2S reacts, gelatin liquefaction, urease test, indoles examination
Test the equal positive, 30 DEG C~35 DEG C of optimum growth temperature, pH=7.
A kind of Dongfanghong bacterium biocontrol agent is by above-mentioned Dongfanghong bacteria strain is activated, prepared by seed culture, productive culture
It obtains, includes the following steps：
(1) it activates：The preservation kind of Dongfanghong bacteria strain by double-layer plate cultivation is cultivated, is cultivated to red single
Bacterium colony occurs；The culture medium used is photosynthetic bacteria solid medium；
(2) seed culture：The red single bacterium colony that step 1 is turned out is accessed in serum bottle, is trained with photosynthetic bacteria liquid
It supports base progress seed culture to exponential phase and obtains bacterium solution；The culture medium used is photosynthetic bacteria liquid culture medium；
(3) productive culture：By the bacterium solution obtained after step 2 seed culture by the inoculation of Photosynthetic bacterium strain total volume 5%
Amount is inoculated into production bottle, carries out productive culture to exponential phase with photosynthetic bacteria liquid culture medium, preferably bacteria concentration is 5
×109Cfu/mL obtains Dongfanghong bacterium biocontrol agent.
Application process of the Dongfanghong bacteria agent in bacterial blight of rice be：By Dongfanghong bacteria agent, the amount of spraying is
120mL per acre is watered 500 times of dilution, is sprayed on rice leaf for the first time in Rise's boot period, is sprayed again after 15 days primary.
As one embodiment of the present of invention：One plant of Dongfanghong bacteria strain, entitled Dongfanghong bacterium I-1-1, in
The deposit number of state's Type Tissue Collection is CCTCC NO:M2012368, it is big that depositary institution address is located at Wuhan, China
It learns, preservation date is September in 2012 26 days.
A kind of Dongfanghong bacterium biocontrol agent, by Dongfanghong bacteria strain is activated, seed culture, productive culture are prepared,
Include the following steps：
(1) it activates：The preservation kind of Dongfanghong bacteria strain by double-layer plate cultivation is cultivated, is cultivated to red single
Bacterium colony occurs；The culture medium used is formulated for photosynthetic bacteria solid medium and is：(NH4) 2SO40.1wt%, Mg
SO40.02wt%, NaHCO30.5wt%, K2HPO40.05wt%, NaCl 0.02wt%, yeast extract 0.15wt% and agar
1.5wt%, pH=7.0.
(2) seed culture：The red single bacterium colony that step 1 is turned out is accessed in serum bottle, is trained with photosynthetic bacteria liquid
It supports base progress seed culture to exponential phase and obtains bacterium solution；The culture medium used is photosynthetic bacteria liquid culture medium, formula
For：(NH4) 2SO40.1wt%, MgSO40.02wt%, NaHCO3 0.5wt%, K2HPO40.05wt%, NaCl
0.02wt%, yeast extract 0.15wt% (being mass fraction), pH=7.0.
(3) productive culture：By the bacterium solution obtained after step 2 seed culture by the inoculation of Photosynthetic bacterium strain total volume 5%
Amount is inoculated into production bottle, carries out productive culture to exponential phase with photosynthetic bacteria liquid culture medium, preferably bacteria concentration is 5
×109Cfu/mL obtains Dongfanghong bacterium biocontrol agent.
As one embodiment of the present of invention：Prevention and control of the Dongfanghong bacteria agent field to bacterial blight of rice
Changsha County spring Hua Zhen is experimental field located at, respectively converts Dongfanghong bacteria agent obtained (5 × 109 cfu/mL of concentration)
Water dilutes 500 times, comparison medicament agricultural streptomycin (72% wettable powder, 15g/ mus), and (MBAMT, 20% can for chemical pesticide
Wet powder, 100g/ mus) it is sprayed on rice for the first time in Rise's boot period, it is sprayed again after 15 days once, each handles 6
Mu, if 3 repetitions.By 5 method sampling survey bacterial blight of rice disease indexs after spraying 15 days at second, and calculate each
From control effect.
Fig. 1 for Dongfanghong bacteria agent in crop field to the control effect statistical chart of bacterial leaf streak of rice, can from Fig. 1
Know：Compared with blank control, the control effect using Dongfanghong bacteria agent, pesticide streptomysin and chemical pesticide is respectively
66.35%th, 76.03% and 79.08%, statistical analysis is found, the preventive effect for spraying above-mentioned 3 medicament does not have significant difference (P<
0.01), show photosynthetic bacteria Dongfanghong bacteria agent as a kind of novel microbial pesticide, there have to bacterial blight of rice to be very good
Control effect.
The basic principles, main features and the advantages of the invention have been shown and described above, for this field
For technical staff, it is clear that the present invention is not limited to the details of above-mentioned exemplary embodiment, and in the spirit without departing substantially from the present invention
Or in the case of essential characteristic, the present invention can be realized in other specific forms.It therefore, in all respects, should all
Regard embodiment as exemplary, and be non-limiting, the scope of the present invention is by appended claims rather than above-mentioned
Illustrate to limit, it is intended that including all changes falling within the meaning and scope of the equivalent requirements of the claims in this hair
In bright.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only
Comprising an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
When considering the specification as a whole, the technical solutions in each embodiment can also be properly combined, forms people in the art
The other embodiment that member is appreciated that.
Claims (5)
1. a kind of Dongfanghong bacteria strain, it is characterised in that：The Dongfanghong bacteria strain is Dongfanghong bacterium I-1-1, is preserved in China
Type Tissue Collection, deposit number are CCTCC NO:M2012368.
2. the Dongfanghong bacteria agent containing bacterial strain described in claim 1.
3. a kind of Dongfanghong bacteria agent according to claim 2, it is characterised in that：The Dongfanghong bacteria agent is by Dongfanghong
Bacteria strain is activated, seed culture, productive culture are prepared.
4. a kind of Dongfanghong bacteria agent according to claim 2, it is characterised in that：Bacteria concentration is 108-1010cfu/mL。
5. bacterial strain according to claim 1 or claim 2~4 any one of them Dongfanghong bacteria agent are harnessed the river anti-
Application on the bacterial blight of rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810202487.XA CN108192851B (en) | 2018-03-13 | 2018-03-13 | Oriental rhodobacter strain, oriental rhodobacter agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810202487.XA CN108192851B (en) | 2018-03-13 | 2018-03-13 | Oriental rhodobacter strain, oriental rhodobacter agent and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108192851A true CN108192851A (en) | 2018-06-22 |
| CN108192851B CN108192851B (en) | 2021-03-19 |
Family
ID=62595121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810202487.XA Active CN108192851B (en) | 2018-03-13 | 2018-03-13 | Oriental rhodobacter strain, oriental rhodobacter agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108192851B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283738A (en) * | 2019-05-22 | 2019-09-27 | 湖南省植物保护研究所 | Photosynthetic bacteria Rhodobacter capsulatus bacterial strain, microbial inoculum medicament and its preparation method and application |
| CN111979143A (en) * | 2020-08-04 | 2020-11-24 | 安徽科技学院 | Lysobacter novel strain and production application thereof |
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2018
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283738A (en) * | 2019-05-22 | 2019-09-27 | 湖南省植物保护研究所 | Photosynthetic bacteria Rhodobacter capsulatus bacterial strain, microbial inoculum medicament and its preparation method and application |
| CN110283738B (en) * | 2019-05-22 | 2020-10-16 | 湖南省植物保护研究所 | Photosynthetic bacterium capsular rhodobacter strain, microbial inoculum medicament, and preparation method and application thereof |
| CN111979143A (en) * | 2020-08-04 | 2020-11-24 | 安徽科技学院 | Lysobacter novel strain and production application thereof |
| CN111979143B (en) * | 2020-08-04 | 2022-04-29 | 安徽科技学院 | Lysobacter strain and production application thereof |
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| Publication number | Publication date |
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| CN108192851B (en) | 2021-03-19 |
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