CN108192851B

CN108192851B - Oriental rhodobacter strain, oriental rhodobacter agent and application thereof

Info

Publication number: CN108192851B
Application number: CN201810202487.XA
Authority: CN
Inventors: 刘勇; 陈岳; 张德咏; 罗源华; 苏品
Original assignee: HUNAN PLANT PROTECTION INSTITUTE; Changsha Agri Biological Fertilizer Technology Development Co ltd
Current assignee: Hunan Xinchangshan Agricultural Development Co ltd
Priority date: 2018-03-13
Filing date: 2018-03-13
Publication date: 2021-03-19
Anticipated expiration: 2038-03-13
Also published as: CN108192851A

Abstract

The invention provides an east red fungus strain, wherein the east red fungus strain is east red fungus I-1-1, is preserved in China center for type culture collection, and has a preservation number of CCTCC NO: M2012368.

Description

Oriental rhodobacter strain, oriental rhodobacter agent and application thereof

Technical Field

The invention relates to the technical field of biological pesticides, and particularly relates to application of a novel microbial agent (an oriental red fungus agent) in prevention and control of bacterial leaf blight of rice.

Background

Bacterial blight, one of the major bacterial diseases of rice, is caused by infection with Xanthomonas oryzae pv. After the leaves are infected by pathogenic bacteria, yellowish green spots are generated at the leaf tips and the edges, pale yellow brown long spots are developed along the veins, and finally, the leaves become grayish white and withered. The diseased plants are easy to fall down, the rice ears are not solid, the influence on the yield is large, once the yield reduction is 10% -20%, and if the yield reduction is serious, the yield reduction can reach 50% -60%, and the safety production of the rice is seriously threatened.

At present, the prevention and control of the bacterial leaf blight of rice mainly depends on resistant variety breeding and chemical pesticide application, and the problems of pesticide residue, disease resistance increase, environmental pollution and the like caused by long-term use of a large amount of chemical pesticide seriously influence the sustainable development of agriculture. And the resistance breeding difficulty is high, the period is long, the corresponding agricultural measures are complex, and the economic investment is large. In recent years, biological control becomes a popular research field for plant disease control due to the characteristics of environmental friendliness, low cost and no pollution, and is also a development trend of current and future plant disease control.

The research shows that the metabolite of the lysobacter contains the phenazine compound which can obviously inhibit the activity of the bacterial blight and the lysobacter has good effect on the bacterial blight in the field. The bacillus can also inhibit the activity of the bacterial blight, and biological tests show that the lesion length is obviously reduced on the rice leaves sprayed with the bacillus. The series of researches show that the biological control of the bacterial leaf blight of the rice has wide application prospect. Photosynthetic Bacteria (PSB) are a general term for a type of Bacteria capable of photosynthesis without oxygen release under anaerobic conditions, researches show that the Photosynthetic Bacteria can activate the immune response of a host and inhibit the growth of pathogenic Bacteria, and have a very good effect on controlling vegetable diseases, but no report has been reported on the aspect of controlling rice bacterial leaf blight.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide an oriental rhodobacter strain to solve the problems in the background technology.

In order to achieve the purpose, the invention is realized by the following technical scheme: the rhodobacter orientalis strain is rhodobacter orientalis I-1-1, is preserved in China center for type culture collection with the preservation number of CCTCC NO: M2012368.

The rhodobacter orientalis microbial inoculum containing the strain.

Furthermore, the oriental red fungus agent is prepared by activating an oriental red fungus strain, culturing seeds and performing production culture.

Further, the concentration of the bacteria is 10⁸-10¹⁰cfu/mL。

The application of the rhodobacter orientalis microbial inoculum in preventing and treating the bacterial leaf blight of rice.

The invention has the beneficial effects that: the production process of the east rhodobacter strain is simple, the culture time is short, the bio-control fungicide can be prepared by a simple, quick and low-cost operation method, the rice bacterial leaf blight can be effectively prevented and treated, and the east rhodobacter strain has the characteristics of environmental friendliness, simplicity and convenience in application, low cost, no pollution, no phytotoxicity to crops and the like.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a statistical chart of the control effect of the rhodobacter orientalis preparation on bacterial leaf blight of rice in a field;

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

The invention provides a technical scheme that: the rhodobacter orientalis strain is rhodobacter orientalis I-1-1, is preserved in China center for type culture collection with the preservation number of CCTCC NO: M2012368.

As an embodiment of the present invention: the said east Monascus strain is east Monascus I-1-1 (Rhodobium orientalis I-1-1), and is preserved in China center for type culture Collection with the preservation number of CCTCC NO: M2012368. The main biological characteristics are as follows: culturing for 4-8 days on a double-layer solid plate culture medium to form red round colonies, wherein the edges of the colonies are neat and smooth, and the diameter of the colonies is 0.2-0.60 mm; culturing in liquid culture medium for 7d to obtain deep red; good growth under anaerobic and micro-aerobic conditions; the biochemical characteristics are that V-P reaction and methyl red reaction are negative, H2S reaction, gelatin liquefaction, urease test and indole test are all positive, the optimal growth temperature is 30-35 ℃, and the pH value is 7.

The anti-biological agent of the east monascus is prepared by activating, seed culturing and production culturing the east monascus strain, and comprises the following steps:

(1) and (3) activation: culturing the preserved species of the east monascus strain according to a double-layer plate culture method until a red single colony appears; the adopted culture medium is a photosynthetic bacteria solid culture medium;

(2) seed culture: inoculating the red single colony cultured in the step 1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; the adopted culture medium is a photosynthetic bacteria liquid culture medium;

(3) production and culture: inoculating the bacterial liquid obtained after seed culture in the step 2 into a production bottle according to the inoculation amount of 5 percent of the total volume of the photosynthetic bacteria strain, and carrying out production culture by using a photosynthetic bacteria liquid culture medium until the logarithmic phase is reached, wherein the preferred bacterial concentration is 5 multiplied by 10⁹cfu/mL to obtain the anti-biological agent of the red-rooted rhodobacter orientalis.

The application method of the rhodobacter orientalis microbial inoculum on the bacterial leaf blight of rice comprises the following steps: spraying the rhodobacter orientalis microbial inoculum with the spraying amount of 120mL per mu, diluting with water by 500 times, spraying the rhodobacter orientalis microbial inoculum on rice leaves for the first time in the booting period of the rice, and spraying the rhodobacter orientalis microbial inoculum once after 15 days.

As an embodiment of the present invention: the name of the rhodobacter orientalis strain is rhodobacter orientalis I-1-1, the preservation number of the rhodobacter orientalis strain in China center for type culture preservation is CCTCC NO: M2012368, the preservation unit address is located at Wuhan university in China, and the preservation date is 9 months and 26 days 2012.

The anti-biological agent is prepared by activating, seed culturing and production culturing of a rhodobacter orientalis strain, and comprises the following steps:

(1) and (3) activation: culturing the preserved species of the east monascus strain according to a double-layer plate culture method until a red single colony appears; the adopted culture medium is a photosynthetic bacteria solid culture medium, and the formula is as follows: (NH4)2so 40.1wt%, Mg so 40.02wt%, nahco30.5wt%, k2 hpo40.05wt%, NaCl 0.02 wt%, yeast extract 0.15 wt%, and agar 1.5 wt%, with pH 7.0.

(2) Seed culture: inoculating the red single colony cultured in the step 1 into a serum bottle, and performing seed culture on a photosynthetic bacteria liquid culture medium to a logarithmic phase to obtain a bacterial liquid; the adopted culture medium is a photosynthetic bacteria liquid culture medium, and the formula is as follows: (NH4)2so 40.1wt%, mgso 40.02wt%, nahco30.5wt%, k2hpo40.05wt%, NaCl 0.02 wt%, yeast extract 0.15 wt% (all mass fractions), pH 7.0.

As an embodiment of the present invention: prevention and control of rice bacterial leaf blight in field by using rhodobacter orientalis fungicide

Experimental sites were established in the town of spring Hua of Changsha county, and the prepared Rhodotorula squarrosporus microbial inoculum (concentration 5X 10) was used⁹cfu/mL) was diluted 500 times with water, and the control agent agricultural streptomycin (72% wettable powder, 15 g/mu), chemical pesticide (bisultap, 20% wettable powder, 100 g/mu) was sprayed on rice for the first time in the booting stage of rice, and sprayed once again after 15 days, with 3 replicates for each treatment of 6 mu. Sampling and investigating the disease index of the rice bacterial leaf blight by a 5-point method 15 days after the second pesticide spraying, and calculating respective control effects.

FIG. 1 is a statistical chart of the control effect of the Rhodobacter orientalis microbial inoculum on the bacterial leaf streak of rice in a field, and it can be known from FIG. 1 that: compared with a blank control, the control effects of the application of the rhodobacter eastern sii microbial inoculum, the pesticide streptomycin and the chemical pesticide are respectively 66.35%, 76.03% and 79.08%, and statistical analysis shows that the control effects of the application of the 3 pesticides have no significant difference (P <0.01), which indicates that the rhodobacter eastern photosynthetic bacteria microbial inoculum as a novel microbial pesticide has a very good control effect on rice bacterial blight.

While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The application of the rhodobacter orientalis fungicide in preventing and treating the bacterial blight of rice is characterized in that the rhodobacter orientalis fungicide contains rhodobacter orientalis I-1-1, is preserved in China center for type culture Collection with the preservation number of CCTCC NO: M2012368, is prepared from rhodobacter orientalis strains through activation, seed culture and production culture, and has the fungus concentration of 10⁸-10¹⁰cfu/mL。