CN108186390B

CN108186390B - Whitening skin-care essence and preparation method thereof

Info

Publication number: CN108186390B
Application number: CN201810236722.5A
Authority: CN
Inventors: 魏有何; 肖业成; 高巧丽; 廖深克
Original assignee: Lianyuan Kangcheng Agricultural Technology Development Co ltd; Lianyuan Kangbiotech Co ltd
Current assignee: Lianyuan Kangbiotech Co ltd; Lianyuan Kangcheng Agriculture and Forestry Technology Development Co.,Ltd.
Priority date: 2018-03-21
Filing date: 2018-03-21
Publication date: 2020-12-29
Anticipated expiration: 2038-03-21
Also published as: CN108186390A

Abstract

The invention discloses a whitening and skin-care essence and a preparation method thereof, wherein the whitening and skin-care essence comprises the following components in percentage by weight: 0.1-10% of neohesperidin dihydrochalcone, 0.1-10% of baicalin, 0.1-5% of daidai pectin, 0.1-2% of dai essential oil, 10-20% of an organic solvent and the balance of water. In the components of the whitening skin-care essence, the neohesperidin dihydrochalcone and the baicalin are compounded according to the weight percentage, so that the active site of tyrosinase can be damaged, the tyrosinase loses the effect on melanin precursor tyrosine, and the purpose of inhibiting melanin formation is achieved. In addition, the neohesperidin dihydrochalcone and baicalin can effectively inhibit MMP-1 gene up-regulation caused by infrared rays, so that the premature senility of human skin can be effectively prevented. The preparation method of the whitening and skin-care essence has the advantages of easily obtained raw materials, low cost, simple method and easy realization of industrial production.

Description

Whitening skin-care essence and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to a whitening and skin-care essence and a preparation method thereof.

Background

Skin whitening is one of the traditional aesthetic standards of Chinese females, many females hope to achieve the whitening effect by using a functional whitening skin care product, the whitening product has great market value at home and abroad, but the infrared ray causes the up-regulation of MMP-1 gene in a human body, the MMP-1 is a regulation gene of collagen degrading enzyme, and the degradation of collagen is a main factor of the premature senility of human skin. Meanwhile, the hyperpigmentation of melanin causes skin problems such as spots, freckles, etc., and affects the skin quality of the skin.

The currently accepted process of melanogenesis can be briefly expressed as: tyrosine-dopa-dopaquinone-dopachrome-dihydroxyindole-ketoindole-melanin, the formed melanin is eumelanin or eumelanin, and the skin pigment mainly consists of the eumelanin. Since the formation of melanin occurs mainly in melanocytes, studies on the mechanism of melanin formation in melanocytes have shown that the formation of melanin is mainly due to the inclusion of four enzymes in melanocytes: tyrosinase, dopachrome tautomerase (TRP-2), peroxidase and DI-IICA oxidase (TRP-1), which stabilize tyrosinase activity, which is different from tyrosinase of mice, mushrooms, etc., in human body, and the above four enzymes act individually or synergistically. Inhibition of various melanin-forming enzymes is important if true skin lightening is to be achieved.

Among the various melanin-forming enzymes mentioned above, tyrosinase is a polyphenol oxidase, which belongs to the class of redox enzymes that catalyze mainly two different types of reactions: hydroxylating the monophenol to generate an ortho-dihydroxy compound, and oxidizing the ortho-diphenol to generate ortho-diphenoquinone. In both reactions, oxygen free radicals participate. Tyrosinase is the main rate-limiting enzyme in the process of melanin formation, and the size of the enzyme activity determines the amount of melanin formation. Therefore, the purpose of inhibiting the formation of melanin can be achieved by destroying the active site of tyrosinase.

Disclosure of Invention

The invention provides a whitening skin-care essence and a preparation method thereof, and aims to solve the technical problems that the skin quality is deteriorated and blackened due to the formation of melanin in the skin, and the skin is senilism and pigmentation are caused by infrared rays.

The technical scheme adopted by the invention is as follows:

the whitening and skin-protecting essence comprises the following components in percentage by weight: 0.1-10% of neohesperidin dihydrochalcone, 0.1-10% of baicalin, 0.1-5% of daidai pectin, 0.1-2% of dai essential oil, 10-20% of an organic solvent and the balance of water.

Further, the paint comprises the following components in percentage by weight: 0.6-8% of neohesperidin dihydrochalcone, 0.1-5% of baicalin, 0.5-5% of daidai pectin, 0.1-2% of dai essential oil, 10-20% of an organic solvent and the balance of water.

Furthermore, the whitening skin care essence also comprises mulberry leaf flavone, and the weight percentage of the mulberry leaf flavone is 0.1-5%.

Furthermore, the whitening skin care essence also comprises mulberry leaf flavone, and the weight percentage of the mulberry leaf flavone is 0.1-3%.

Further, the whitening and skin-care essence also comprises the following components in percentage by weight: 0.1 to 5 percent of phloretin and 0.1 to 5 percent of lycium barbarum polysaccharide.

Further, the whitening and skin-care essence also comprises the following components in percentage by weight: 0.5 to 5 percent of phloretin and 0.1 to 3 percent of lycium barbarum polysaccharide.

Further, the organic solvent is one or more of propylene glycol, glycerol, butanediol and diethylene glycol.

According to another aspect of the invention, a preparation method of the whitening and skin-care essence is also provided, and the preparation method comprises the following steps: placing an organic solvent in a shearing machine, adding daidai pectin and water in the shearing process, then adding neohesperidin dihydrochalcone and baicalin, and finally adding dai essential oil to obtain the whitening and skin-care essence.

The invention has the following beneficial effects:

in the components of the whitening and skin-care essence provided by the invention, the neohesperidin dihydrochalcone and the baicalin are compounded, so that destructive inhibition can be generated on tyrosinase, namely, the active part of the tyrosinase is damaged, and the tyrosinase loses the effect on melanin precursor tyrosine, thereby achieving the purpose of inhibiting melanin formation. Meanwhile, the neohesperidin dihydrochalcone and baicalin can effectively inhibit MMP-1 gene up-regulation caused by infrared rays, so that the premature senility of human skin can be effectively prevented. In addition, the daidaidai essential oil is added, so that the whitening and skin-care essence has a stronger antibacterial effect; the citrus aurantium pectin is an antidote and a prophylactic agent for heavy metals, can reduce the content of heavy metals introduced by air, food and other cosmetics in skin, and can prolong the antibacterial action and the bacteriostatic cycle of citrus aurantium essential oil when combined with the citrus aurantium essential oil; the solubility of the neohesperidin dihydrochalcone and the baicalin in a normal-temperature water solution is limited, the neohesperidin dihydrochalcone and the baicalin are easy to separate out to influence the whitening effect, and the addition of the citrus aurantium pectin can enhance the stability of the whole system and further enhance the whitening effect of the skin care essence.

The preparation method of the whitening and skin-care essence provided by the invention has the advantages of easily obtained raw materials, low cost, simple method and easy realization of industrial production. The traditional preparation method of the essence comprises the steps of heating a system to improve the solubility of each component, reducing the temperature of the system, and then performing split charging and blending. The traditional method wastes energy sources, has long preparation period, and can damage the effective components of each component in the heating process to a certain extent, thereby influencing the whitening effect of the essence. The invention utilizes the high-speed shearing machine to shear and dissolve each component, avoids the heating process of the system, does not damage each component, reduces the energy loss and shortens the preparation period.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail with reference to examples.

The preferred embodiment of the invention provides a whitening and skin-care essence which comprises the following components in percentage by weight: 0.1-10% of neohesperidin dihydrochalcone, 0.1-10% of baicalin, 0.1-5% of daidai pectin, 0.1-2% of dai essential oil, 10-20% of an organic solvent and the balance of water.

In the components of the whitening and skin-care essence, neohesperidin dihydrochalcone is one of plant-derived flavonoid compounds, and is mainly used as a sweetener in the food industry at present. Researches show that the neohesperidin dihydrochalcone has better effect of inhibiting the neuraminidase than beta-arbutin, kojic acid and VC. Neohesperidin dihydrochalcone is a glycoside flavonoid, and a flavonoid compound is a natural substance extracted from plants. Many flavone glycosides are peculiar to Rutaceae plants, and the synthesis starting material of neohesperidin dihydrochalcone is neohesperidin which is extracted and refined from bitter orange. The literature indicates that the tyrosinase inhibition rate of the neohesperidin dihydrochalcone is the highest compared with the beta-arbutin, VC and kojic acid. The inhibitory capacity of the neohesperidin dihydrochalcone on melanin generation is close to that of VC and kojic acid and is 3 times of that of beta-arbutin, so that the neohesperidin dihydrochalcone is a novel whitening agent with application potential and can be used as a main active ingredient in the development of novel whitening skin care products.

In the components of the whitening skin-care essence, baicalin is a flavonoid compound extracted from astragalus, and the antioxidant activity of scutellaria is proved to be greater than that of vitamin E by an in-vitro oxygen radical absorption capacity method, so that the baicalin is a natural strong antioxidant except vitamin E, vitamin C and carotene. The baicalin can inhibit lipid peroxidation of mouse brain cells induced by ascorbic acid and ferrous sulfate, and reduce oxygen consumption of brain nerves stimulated by ADP by using malic acid and glutamic acid as substrates, thereby reducing lipid peroxidation.

Among the components of the whitening skin-care essence, the method for extracting the daidai essential oil comprises the steps of extracting the dai from dai, wherein the extraction conditions are that the temperature is 40 ℃, the pressure is 35Mpa, the carbon dioxide flow is 20kg/h, the bulk density of the dai is 0.33g/ml, the extract yield is 3.0-3.3%, and the extracted dai essential oil is light yellow clear liquid in appearance and pure in fragrance. The main active ingredient of daidaidai essential oil is limonene, and pharmacological experiments prove that daidai essential oil is a natural bacteriostatic agent and has a strong antibacterial effect.

Among the components of the whitening skin care essence, the method for extracting citrus aurantium pectin comprises the steps of taking citrus aurantium flavone extraction residues, adding 10 times of pure water, adjusting the pH value to 1.5-2 by using citric acid, extracting for 2 hours at 85 ℃, filtering, concentrating filtrate to 2 times of the extraction residues, adjusting the alcohol content to 55% -60% by using absolute ethyl alcohol, collecting precipitates, drying in vacuum, and crushing to obtain the citrus aurantium pectin, wherein the extracted citrus aurantium pectin is white powder. The citrus aurantium pectin is a purely natural stabilizer and thickener, is also an antidote and a prophylactic agent for heavy metals, can prolong the action of antibiotics, and is matched with citrus aurantium essential oil to obviously enhance the antibacterial capacity and the antibacterial period of the citrus aurantium essential oil.

In the components of the whitening and skin-care essence, the neohesperidin dihydrochalcone and the baicalin are compounded according to the weight percentage, so that destructive inhibition can be generated on tyrosinase, namely, the active part of the tyrosinase is damaged, the tyrosinase loses the effect on melanin precursor tyrosine, and the purpose of inhibiting melanin formation is achieved. Meanwhile, the neohesperidin dihydrochalcone and baicalin can effectively inhibit MMP-1 gene up-regulation caused by infrared rays, so that the premature senility of human skin can be effectively prevented. In addition, the daidaidai essential oil is added, so that the whitening and skin-care essence has a stronger antibacterial effect; the citrus aurantium pectin is an antidote and a prophylactic agent for heavy metals, can reduce the content of heavy metals introduced by air, food and other cosmetics in skin, and can prolong the antibacterial action and the bacteriostatic cycle of citrus aurantium essential oil when combined with the citrus aurantium essential oil; the solubility of the neohesperidin dihydrochalcone and the baicalin in a normal-temperature water solution is limited, the neohesperidin dihydrochalcone and the baicalin are easy to separate out to influence the whitening effect, and the addition of the citrus aurantium pectin can enhance the stability of the whole system and further enhance the whitening effect of the skin care essence.

Preferably, the whitening and skin-care essence comprises the following components in percentage by weight: 0.6-8% of neohesperidin dihydrochalcone, 0.1-5% of baicalin, 0.5-5% of daidai pectin, 0.1-2% of dai essential oil, 10-20% of an organic solvent and the balance of water. More preferably, the whitening and skin-care essence comprises the following components in percentage by weight: 0.6-5% of neohesperidin dihydrochalcone, 0.1-1% of baicalin, 3-5% of daidaidai pectin, 0.1-2% of daidai essential oil, 10-20% of an organic solvent and the balance of water.

The components in the whitening and skin-care essence are compounded according to the preferable weight percentage, so that tyrosinase can be better inhibited, and the formation of melanin is inhibited; in addition, the preferable proportion of the neohesperidin dihydrochalcone and the baicalin can most effectively inhibit the MMP-1 gene up-regulation caused by infrared rays, thereby generating better inhibiting effect on the skin premature senility of human bodies; the optimal proportion of daidaidai essential oil and daidaidai pectin enables the antibacterial effect of the skin care essence to be strongest, and the stability of each component in the system to be enhanced to the greatest extent, so that the whitening effect is optimal.

Preferably, the whitening and skin-care essence also comprises mulberry leaf flavone, and the weight percentage of the mulberry leaf flavone is 0.1-5%. More preferably, the weight percentage is 0.1-3%. More preferably, the weight percentage is 0.1 to 1 percent.

In the components of the whitening skin care essence, the mulberry flavone is a flavonoid compound extracted from mulberry, and can achieve antioxidant activity by improving SOD activity, reducing LPO content and changing GSH-PX level. The compounding of the mulberry leaf flavone, the neohesperidin dihydrochalcone and the baicalin can enhance the capability of destroying the active site of tyrosinase, so that the tyrosinase loses the effect of strengthening melanin precursor tyrosine, and the whitening effect of the whitening and skin-care essence is enhanced.

Preferably, the whitening and skin-care essence also comprises the following components in percentage by weight: 0.1 to 5 percent of phloretin and 0.1 to 5 percent of lycium barbarum polysaccharide. Preferably, the whitening and skin-care essence also comprises the following components in percentage by weight: 0.5 to 5 percent of phloretin and 0.1 to 3 percent of lycium barbarum polysaccharide. More preferably, the whitening and skin-care essence further comprises the following components in percentage by weight: phloretin 1-5 wt% and wolfberry fruit polysaccharide 0.1-1 wt%.

In the components of the whitening skin-care essence, phloretin is an aglycon part of phlorizin and is separated from the phlorizin by adding acid and hydrolyzing; or synthesized by corresponding chemical processes. Phloretin can inhibit excessive secretion of sebaceous gland, and can be used for treating acne with excessive secretion; can inhibit activity of melanocyte, and has strong effect in eliminating various skin color spots.

In the components of the whitening and skin-care essence, the lycium barbarum polysaccharide is a polysaccharide compound extracted from lycium barbarum. Epidemiological data prove that the occurrence and development of skin diseases such as acne, atopic dermatitis, polymorphous light eruption, scleroderma, epidermal tumor and the like are all related to oxidative stress injury, and lycium barbarum polysaccharide can relieve the oxidative stress injury of cells through an antioxidant mechanism, maintain the dynamic balance of skin oxidation and antioxidant to repair and protect the cells.

In the components of the whitening skin care essence, when the phloretin, the lycium barbarum polysaccharide and the mulberry flavone are compounded, the purpose of inhibiting melanin formation can be achieved by inhibiting the biosynthesis of tyrosinase or substituting a substrate of the tyrosinase. And the five components are combined for use by compounding with neohesperidin dihydrochalcone and baicalin, so that the source of melanin generation is thoroughly cut off, and the effect of whitening skin is achieved.

Preferably, the organic solvent is one or more of propylene glycol, glycerol, butanediol and diethylene glycol. More preferably, the organic solvent is propylene glycol. The organic solvent is beneficial to fully dissolving each component in the whitening and skin-care essence, so that the synergistic effect of each component is brought into play. The five main components in the whitening and skin-care essence can achieve good dissolving effect in propylene glycol, and the toxicity and the irritation are very small, so that a victim is not found.

Preferably, the water used in the whitening and skin-care essence is deionized water, and more preferably secondary purified water.

According to another aspect of the present invention, there is also provided a preparation method of the whitening and skin-care essence, comprising the following steps: placing an organic solvent in a shearing machine, adding daidai pectin and water in the shearing process, then adding neohesperidin dihydrochalcone and baicalin, and finally adding dai essential oil to obtain the whitening and skin-care essence. When the whitening skin care essence also comprises mulberry leaf flavone, phloretin and lycium barbarum polysaccharide components, the mulberry leaf flavone, phloretin and lycium barbarum polysaccharide components are added together with neohesperidin dihydrochalcone and baicalin and then cut.

The preparation method of the whitening and skin-care essence has the advantages of easily obtained raw materials, low cost, simple method and easy realization of industrial production. The traditional preparation method of the essence comprises the steps of heating a system to improve the solubility of each component, reducing the temperature of the system, and then performing split charging and blending. The traditional method wastes energy sources, has long preparation period, and can damage the effective components of each component in the heating process to a certain extent, thereby influencing the whitening effect of the essence. The invention utilizes the high-speed shearing machine to shear and dissolve each component, avoids the heating process of the system, does not damage each component, reduces the energy loss and shortens the preparation period.

Examples

The chemicals are commercially available in the following examples.

Example 1

The whitening and skin-protecting essence comprises the following components in percentage by weight: 2% of neohesperidin dihydrochalcone, 1% of baicalin, 0.5% of mulberry leaf flavone, 3% of phloretin, 0.5% of lycium barbarum polysaccharide, 15% of propylene glycol, 5% of daidaidai pectin, 1% of daidai essential oil and 72% of secondary purified water.

The preparation method of the whitening and skin-care essence comprises the following steps: placing propylene glycol in a high-speed shearing machine for shearing, wherein the shearing speed is 15000 r/min, slowly adding citrus aurantium pectin in the shearing process, slowly adding secondary purified water after shearing at normal temperature for 10min, then adding neohesperidin dihydrochalcone, baicalin, mulberry leaf flavone, phloretin and lycium barbarum polysaccharide, dissolving after shearing at normal temperature for 20min, and finally adding citrus aurantium essential oil and shearing for 10min to obtain the whitening and skin-care essence.

The method comprises the steps of taking human skin fibroblasts, and testing the inhibition effect of the 5 main components in the whitening and skin-care essence after treatment and the composition prepared in the example 1 on MMP-1 gene up-regulation after infrared light irradiation by utilizing Hydrosun 500H IRA equipment. The treatment of the 5 main components is as follows:

baicalin: diluting baicalin dissolved in DMSO at 44.8mmol/ml to 10umol/ml and 25umol/ml respectively;

phloretin: diluting phloretin 50mmol/ml dissolved in DMSO to 1umol/ml, 5umol/ml, 10umol/ml respectively;

and (3) mulberry leaf flavone: diluting folium Mori flavone 50mmol/ml dissolved in DMSO to 5umol/ml and 10umol/ml respectively;

wolfberry polysaccharide: diluting 50mmol/ml Lycium barbarum polysaccharides dissolved in DMSO to 5umol/ml and 10umol/ml respectively;

neohesperidin dihydrochalcone: 710mmol/ml neohesperidin dihydrochalcone dissolved in DMSO is respectively diluted to 100umol/ml, 500umol/ml and 1 mmol/ml.

Composition proportioned in example 1: 50mmol/ml of the composition dissolved in DMSO was diluted to 5. mu. mol/ml and 10. mu. mol/ml, respectively.

Before testing, human skin fibroblasts were incubated for 24h at the indicated concentrations of each of the above ingredients, followed by 360J/cm²And the infrared ray is irradiated for 24 hours, and the infrared ray is proved to be capable of inducing gene expression without damaging the cell function. Subsequently, RNA was extracted and MMP-1mRNA was analyzed. The analysis results are shown in table 1. As is clear from the analysis data in Table 1, the compositions of 5 main components and the composition prepared in example 1 were able to inhibit the infrared light-induced MMP-1 gene up-regulation.

TABLE 15 inhibition of MMP-1 gene up-regulation by compositions of the major ingredients and the ratios of examples 1-5

Example 2

The whitening and skin-protecting essence comprises the following components in percentage by weight: 8% of neohesperidin dihydrochalcone, 5% of baicalin, 0.1% of mulberry leaf flavone, 0.1% of phloretin, 0.1% of lycium barbarum polysaccharide, 15% of propylene glycol, 5% of daidaidai pectin, 1% of dai essential oil and 65.7% of secondary purified water.

The preparation method of the whitening and skin-care essence is the same as that of the embodiment 1.

The inhibition effect of the composition prepared in example 2 on the up-regulation of MMP-1 gene after infrared light irradiation is shown in Table 1.

Example 3

The whitening and skin-protecting essence comprises the following components in percentage by weight: 2% of neohesperidin dihydrochalcone, 1% of baicalin, 5% of mulberry leaf flavone, 5% of phloretin, 5% of lycium barbarum polysaccharide, 15% of propylene glycol, 5% of daidaidai pectin, 2% of daidai essential oil and 60% of secondary purified water.

The inhibition effect of the composition prepared in example 3 on the up-regulation of MMP-1 gene after infrared light irradiation is shown in Table 1.

Example 4

The whitening and skin-protecting essence comprises the following components in percentage by weight: 8% of neohesperidin dihydrochalcone, 5% of baicalin, 3% of mulberry leaf flavone, 5% of phloretin, 3% of lycium barbarum polysaccharide, 20% of propylene glycol, 5% of daidaidai pectin, 2% of daidai essential oil and 49% of secondary purified water.

The inhibition effect of the composition prepared in example 4 on the up-regulation of MMP-1 gene after infrared light irradiation is shown in Table 1.

Example 5

The whitening and skin-protecting essence comprises the following components in percentage by weight: 10% of neohesperidin dihydrochalcone, 10% of baicalin, 5% of mulberry leaf flavone, 5% of phloretin, 5% of lycium barbarum polysaccharide, 20% of propylene glycol, 5% of daidaidai pectin, 2% of daidai essential oil and 38% of secondary purified water.

The inhibition effect of the composition prepared in example 5 on the up-regulation of MMP-1 gene after infrared light irradiation is shown in Table 1.

In order to verify the whitening effect of the product, the following comparative test formula is carried out for a comparative experiment:

formula 1: the mulberry leaf flavone, phloretin and lycium barbarum polysaccharide are respectively replaced by the same amount of second-level purified water, and the composition and preparation method of other components are the same as those in example 1.

And (2) formula: neohesperidin dihydrochalcone and baicalin were replaced with the same amount of second-stage purified water, and the composition and preparation method of the other components were the same as those in example 1.

And (3) formula: the composition and preparation of all components are the same as in example 1.

Volunteers: randomly selecting 30 healthy male and female volunteers, wherein the male and female volunteers are 18-65 years old, have no serious system diseases, no immune defects or autoimmune diseases, have no allergic diseases, do not use various cosmetics and the like during a test period, and can finish the use of specified samples according to experimental requirements. The experimental method comprises the following steps: the volunteers are divided into 3 groups according to the formula, 10 persons are in each group, the 3 groups of the formula are subjected to a trial test on the volunteers for 28 days, the volunteers are respectively used for 1 time in the morning, at noon and at night, and the trial part is the face. Testing an instrument: a skin elasticity CotometerMPA580 host manufactured by CK company, germany; skin melanin mexamettmx 18 test probe manufactured by CK company, germany. Face test conditions: the face test was performed indoors with no intense sunlight or direct lighting. Whitening products (including other cosmetics, external medicines or internal health care products) cannot be used 2-3 days before the test. The subjects required a uniform cleansing of facial skin and a clean wipe with dry tissue prior to testing.

Based on the principle of spectral absorption, the content of melanin in the skin is determined by measuring the reflection amount of light with a specific wavelength irradiated on the skin of a human body. The test probe of the pigment detector consists of a light source emitter and a light source receiver, and a spring is additionally arranged to keep the pressure on the skin constant during detection. The transmitter of the probe emits light with three wavelengths of 568nm, 660nm and 880nm to the surface of the skin, and the receiver measures the light reflected by the skin. Since the amount of emitted light is constant, the amount of light absorbed by the skin can be measured, and the amount of melanin in the skin can be measured. The data in table 2 are the skin melanin content determined.

TABLE 2 comparison of melanin content (%)

The data from the 3 formulations above were subjected to paired T-test using span 17.0, with the following results:

formula 1:

paired sample statistics

Correlation coefficient of paired samples

		N	Correlation coefficient	Sig.
					Pair 1	Day 1&Day 28	10	.920	.000

Paired sample testing

From the above data, it can be seen that the mean value of skin melanin content of the volunteers after the 28-day test period of formula 1 decreased by 13.4, and the skin whitening tendency was significant (P < 0.05).

And (2) formula:

paired sample statistics

Correlation coefficient of paired samples

		N	Correlation coefficient	Sig.
					Pair 1	Day 1&Day 28	10	.787	.007

Paired sample testing

From the above data, it can be seen that the mean value of skin melanin content of volunteers after the 28-day test period of formula 2 decreased by 11.4, and the skin whitening trend was significant (P < 0.05).

And (3) formula:

paired sample statistics

Correlation coefficient of paired samples

		N	Correlation coefficient	Sig.
					Pair 1	Day 1&Day 28	10	.795	.006

Paired sample testing

From the above data, it can be seen that the mean value of skin melanin content of volunteers after the 28-day test period of formulation 3 decreased by 22, and the skin whitening tendency was significant (P < 0.05).

The above experimental results show that the skin melanin content of the volunteers is reduced after the test period of 28 days by the 3 test formulas, and the skin whitening trend is obvious, wherein the skin melanin content of the volunteers is reduced most obviously after the test period of 28 days by the formula 3, and the effect is obviously better than that of the former two groups of formulas. Consumer tests show that the whitening and skin-care essence has an obvious effect on whitening skin by combining 5 main components of neohesperidin dihydrochalcone, baicalin, mulberry leaf flavone, phloretin and lycium barbarum polysaccharide, and the whitening effect is superior to that of a formula in which neohesperidin dihydrochalcone and baicalin are singly combined or a formula in which mulberry leaf flavone, phloretin and lycium barbarum polysaccharide are singly combined.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The whitening and skin-care essence is characterized by comprising the following components in percentage by weight:

8% of neohesperidin dihydrochalcone, 5% of baicalin, 3% of mulberry leaf flavone, 5% of phloretin, 3% of lycium barbarum polysaccharide, 20% of propylene glycol, 5% of daidaidai pectin, 2% of daidai essential oil and 49% of secondary purified water;

the preparation method of the whitening and skin-care essence comprises the following steps:

placing propylene glycol in a high-speed shearing machine for shearing, wherein the shearing speed is 15000 r/min, slowly adding citrus aurantium pectin in the shearing process, slowly adding secondary purified water after shearing for 10min at normal temperature, then adding neohesperidin dihydrochalcone, baicalin, mulberry leaf flavone, phloretin and lycium barbarum polysaccharide, dissolving after shearing for 20min at normal temperature, and finally adding citrus aurantium essential oil and shearing for 10min to obtain the whitening and skin-care essence.