CN108186373A - A kind of production method of the hypha,hyphae flexible material as facial mask cloth - Google Patents
A kind of production method of the hypha,hyphae flexible material as facial mask cloth Download PDFInfo
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- CN108186373A CN108186373A CN201810161733.1A CN201810161733A CN108186373A CN 108186373 A CN108186373 A CN 108186373A CN 201810161733 A CN201810161733 A CN 201810161733A CN 108186373 A CN108186373 A CN 108186373A
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- mycelia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention belongs to technical field of material, and in particular to a kind of production method of the hypha,hyphae flexible material as facial mask cloth includes the following steps：1) expand numerous parent species；2) liquid spawn is prepared；3) it is diluted with water；4) mycelium pellet is broken up；5) bacteria suspension is dispensed；6) quiescent culture；7) film is dragged for；8) continue to cultivate.The present invention has easy to operate, safe green relative to the prior art, and special sterilization anti-inflammatory efficacy, good toughness, degradability, skin affinity is good, cost-effective grade very excellent effect, conducive to commercial application.
Description
Technical field
The invention belongs to technical field of material, and in particular to a kind of hypha,hyphae flexible material as facial mask cloth
Production method.
Background technology
Fungi is a monoid very big in living nature, and there are about more than 120,000 to plant for the fungi being described in the world, biology
About 40,000 kinds of estimation China of family.Eumycota is usually divided into Mastigomycotina, Zygomycotina, Ascomycotina, load by people
Daughter bacteria subphylum and Deuteromycotina.Wherein, Basidiomycotina fungi is commonly referred to as basidiomycetes, is monoid most high in fungi,
Most kinds have edible and medical value.Basidiomycetes is all made of the mycelium of many cells.Some researches show that many loads
Bacterium mycelium contains multiple nutritional components, such as polysaccharide, aliphatic acid, polypeptide, steroid, triterpene, these ingredients have anticancer, kill
Bacterium, anti-inflammatory, strengthen immunity effect.Wherein, the effect of polysaccharide constituents is more notable.In addition, since many basidiomycetes all have
The advantages of mycelium is flourishing, and mycelial growth is rapid, kink ability is strong, while to contain a large amount of fungies more for hyphal cell pars intramuralis
Sugar has fabulous cementing effect, so under conditions of nutritional sufficiency, mycelium can twist together into complicated netted knot
Structure.
Basidiomycetes strain mainly exists in the form of two kinds of solid spawn and liquid spawn, and liquid spawn due to purity is high,
The advantages that fast, production technology is easy, with short production cycle is sprouted, is widely used, but is also only applied to edible fungus culturing and guarantor
Strong product production field.
At present, most common facial mask cloth is exactly non-woven fabrics on the market, although such material manufacture craft is easy, price is low
It is honest and clean, but it is through the multiple technique such as high-temperature fusion, spinneret by a variety of chemical substances, and hot pressing is batched continuous one-step and is produced
, easily cause skin sensitive, and it is bad with skin affinity, and non-woven fabrics facial mask there is also " back suction " phenomenon.
Invention content
In view of the problems existing in the prior art, screening growth is rapid, kink ability is strong, mycelium nutritional ingredient or effect into
The high specific fungi of point content, using liquid spawn make it is a kind of can be used for production facial mask cloth it is safe and environment-friendly, green, have
The flexible material of special efficacy, be facial mask field or even flexible material field a big innovation and basidiomycetes field it is another
Innovation and application.
The present invention provides that a kind of growth is rapid, kink ability is strong, mycelium nutritional ingredient according to aforementioned creative utilization thinking
Or the specific fungi that functional component content is high, flexible material is made by its liquid spawn, the material is degradable, it is environmentally protective,
It is non-toxic to humans, there are the special efficacies such as anticancer, sterilization, anti-inflammatory, strengthen immunity, except the application for increasing liquid spawn
Outside direction, the present situation that facial mask cloth industry material is single, effect is undesirable currently on the market is further improved, the development for facial mask provides
One new direction.In addition, since the flexibility of such material is preferable, degradability is higher, it can also be applied to making plastics
The products such as bag, disposable dishes, so as to preferably solve the problems, such as that environmental pollution is serious.
The specific technical solution that the present invention uses is a kind of making side of the hypha,hyphae flexible material as facial mask cloth
Method includes the following steps：
1) expand numerous parent species：Choose the healthy and strong dense test tube stock of cleaning-less bacteria infection, mycelia, in aseptic inoculation box, picking 1
Block 5mm × 5mm sizes and parent species without culture medium are inoculated on the test tube slant culture medium of sterilizing cooling, in constant incubator
Inside cultivated；
2) liquid spawn is prepared：In gnotobasis, 2-3 block 5mm × 5mm sizes are taken and without the numerous parent species of expansion of culture medium
It is inoculated in the fluid nutrient medium of 100mL sterilizing coolings, gas bath shaking table culture；
3) it is diluted with water：Sterile water is added in liquid spawn to be diluted；
4) mycelium pellet is broken up：Using magnetic stirring apparatus treatment liquid strain 30min or so, until mycelium pellet therein is complete
It breaks up in portion；
5) bacteria suspension is dispensed：Gained bacteria suspension is dispensed to special container, container there must be ventilation component, and ensure
The sterile air being passed through；The volume for adding in bacteria suspension is the 1/3-1/2 of overall vessel volume；
6) quiescent culture：Container is placed in quiescent culture in gnotobasis；
7) film is dragged for：The mycelia film for growing completion is pulled out and (needs sterile working) with particular tools such as tweezers, for facial mask cloth
Etc. flexible material products preparation；
8) continue to cultivate：The bacteria suspension for having dragged for mycelia film continues quiescent culture.
Preferably, in the step 1) of the method, the strain of the inoculation is basidiomycetes, has mycelium prosperity, growing way
Well, the characteristics of kink power is strong, and the nutritional ingredients such as polysaccharide, aliphatic acid, polypeptide, steroid, triterpene are high in mycelium.
Condition of culture --- cultivation temperature：22-28 DEG C, 7-10 days mycelia of culture can cover with inclined-plane.The condition of culture
Advantage is：(1) spawn activity is higher；(2) growth rate is fast；(3) strain quality is high.
Mother culture based formulas：" potato+cotton seed hulls " extracting solution 1.0L (potato 200.0g, cotton seed hulls 20.0g), Portugal
Grape sugar 20.0g, agar 20.0g, pH are natural.After packing, 121 DEG C of high pressure steam sterilization 30min are cooled down spare.In this culture medium
In, the growth of strain is most vigorous, strain quality highest.
PH refers to that this culture medium makes according to the method naturally, without adjusting pH value.
Preferably, in the step 2) of the method, the condition of culture of the liquid spawn --- temperature：22-28 DEG C, turn
Speed：200-220rpm；Culture can obtain bacterium solution clear, bacterium thick flavor, the liquid spawn full of mycelium pellet in 7 days or so.
Fluid nutrient medium production method is as follows：
Stirring in 25.0g corn flour, 15.0g beancake powder cold water is not had into block, is heated to boiling, boils 10min, during which constantly stir
It mixes, then adds in 20.0g sucrose, 2.0g potassium dihydrogen phosphates, 1.0g magnesium sulfate, vitamin B11, moisturizing to 1.0L, stirring mixes
It is even.After packing, 121 DEG C of high pressure steam sterilization 30min are cooled down spare.
Under above-mentioned condition of culture and material condition, mycelium pellet content highest, the seed production efficiency highest of liquid spawn.
Preferably, in the step 3) of the method liquid spawn dilution ratio --- the volume of liquid spawn and sterile water
Than being 1：3.
Preferably, in the step 6) of the method quiescent culture condition --- cultivation temperature：20-28℃；Cultivate 4 days left sides
The right side can grow mycelia film.
Preferably, in the step 8) of the method quiescent culture condition --- cultivation temperature：20-28℃；Continue culture 4
It or so is rear and can grow the mycelia film of step 6), and so repeatedly, a container can produce 10-20 pieces using a collection of bacteria suspension
Mycelia film.
Another object of the present invention is the provision of a kind of hypha,hyphae flexible material as facial mask cloth, the flexible material
It makes to obtain by aforementioned production method.
The present invention includes relative to the advantageous effect of the prior art：
It is 1. easy to operate：Technological process of the present invention is easy, and operation is relatively easy；
2. safe green：The material of mycelia film flexible material obtained is entirely the net formed by Basidiomycetes mycelium kink
Shape structure, without any chemical substance, edible is non-toxic to humans；
3. special efficacy：Many Basidiomycetes myceliums contain multiple nutritional components, as polysaccharide, aliphatic acid, polypeptide, steroid,
Triterpene etc., these ingredients have the function of anticancer, sterilization, anti-inflammatory, strengthen immunity, so, flexible material obtained also has
Effect as described above especially sterilizes anti-inflammatory efficacy and this kind of material is caused to become being preferably selected for facial mask cloth；
4. good toughness：Mycelia film toughness obtained is good, is not easy to tear, and can also be applied to the products such as substituted for plastic bag；
5. degradability：Discarded mycelia film can be utilized for 30 days after returning soil by the microorganism decomposition in soil, and can be with
Increase soil fertility, thus it is environmentally safe, belong to environmentally friendly material；
6. skin affinity is good：Natural mycelia film is fine and smooth soft, extremely affine skin；
It is 7. cost-effective：It is easy to operate, and it is commonly used equipment to operate equipment, can be made by oneself.
Specific embodiment
Below in conjunction with specific example, the invention will be further described, but the present invention is not limited to this.
Embodiment 1：Ganoderma lucidum
Present embodiment discloses a kind of production methods of the hypha,hyphae flexible material as facial mask cloth, and idiographic flow is such as
Under：
1) expand numerous parent species：
" potato+cotton seed hulls " mother culture media 100mL is prepared, is dispensed to 7 test tubes, 15mL/ branch, 121 DEG C of high pressures are steamed
After vapour sterilizing 30min, inclined-plane is put.After cooling, the healthy and strong dense ganoderma lucidum test tube stock of cleaning-less bacteria infection, mycelia is chosen, sterile
In inoculating hood, 1 piece of 5mm × 5mm size of picking and the parent species without culture medium are inoculated into the test tube slant culture medium of sterilizing cooling
On, it is cultivated in constant incubator, cultivation temperature：25 DEG C, 7 days mycelia of culture can cover with inclined-plane.
2) liquid spawn is prepared：
" corn flour+beancake powder " fluid nutrient medium 1L is prepared, is dispensed into the triangular flask of 250mL, every bottle of 100mL, 121 DEG C
High pressure steam sterilization 30min.After cooling, in gnotobasis, 2 pieces of 5mm × 5mm sizes of picking and without the numerous mother of expansion of culture medium
Kind is inoculated in the fluid nutrient medium of sterilizing cooling, gas bath shaking table culture, condition of culture --- temperature：25 DEG C, rotating speed：200rpm.
Culture can obtain color clarification, bacterium thick flavor, the liquid spawn full of white hypha ball in 7 days.
3) it is diluted with water：
It is 1 according to volume ratio：3 add sterile water to be diluted gained liquid spawn, and 4L liquid spawns are obtained；
4) mycelium pellet is broken up：
Diluted liquid spawn 35min is handled using magnetic stirring apparatus, until mycelium pellet therein is all broken up；
5) bacteria suspension is dispensed：
Gained bacteria suspension is dispensed to sterile square container, size 50cm × 50cm × 5cm, container must have logical
Gas element, and ensure the sterile air being passed through.The volume for adding in bacteria suspension is 4L.
6) quiescent culture：
Container is placed in gnotobasis and is left to ferment, cultivation temperature：20-25℃.It cultivates 4 days, is on bacteria suspension surface
The mycelia film that one layer pure white, fine and close, soft, toughness is strong can be grown.
7) film is dragged for：
In an aseptic environment, the mycelia film for growing completion is pulled out with particular tools such as tweezers, it is specific for facial mask cloth etc.
The preparation of product.
8) continue to cultivate：
The bacteria suspension for having dragged for mycelia film continues quiescent culture, can grow the mycelia film of same performance after 4 days again, so anti-
Multiple, a container can produce 15 mycelia films using a collection of bacteria suspension.
The mycelia fiber of gained mycelia film is very thin, adsorptivity is very good, and a diameter of 2-100nm, about hair 1/1000 are thin,
It is the 1/133 of traditional non-woven cloth, sulci of skin can be goed deep into, hold on to skin cell.In addition, the flexible material is banketed, after 30 days
It can be utilized by the microorganism decomposition in soil, and soil fertility can be increased.
Mycelia film obtained is through specific cutting, you can for the making of facial mask.
Embodiment 2：Oyster mushroom
1) expand numerous parent species：
" potato+cotton seed hulls " parent species slant medium is prepared, eugonic oyster mushroom test tube stock is then chosen, in nothing
In bacterium inoculating hood, 1 piece of 5mm × 5mm size of the picking and parent species without culture medium are inoculated on test tube slant culture medium, in constant temperature
Incubator is cultivated, cultivation temperature：It 24 DEG C, cultivates 8 days, inclined-plane can cover with pure white, dense, sturdy
Mycelia.
2) liquid spawn is prepared：
" corn flour+beancake powder " fluid nutrient medium 500mL is prepared, is dispensed into the triangular flask of 250mL, every bottle of 100mL,
121 DEG C of high pressure steam sterilization 30min.After cooling, in gnotobasis, 2 pieces of 5mm × 5mm sizes of picking and without culture medium
Expand the fluid nutrient medium that numerous parent species are inoculated in sterilizing cooling, gas bath shaking table culture, condition of culture --- temperature：24 DEG C, rotating speed：
200rpm.Culture can obtain bacterium solution clarification, bacterium thick flavor, the liquid spawn full of mycelium pellet in 7 days.
3) it is diluted with water：
It is 1 according to volume ratio：3 add sterile water to be diluted gained liquid spawn, and 2L liquid spawns are obtained；
4) mycelium pellet is broken up：
Diluted liquid spawn 20min is handled using magnetic stirring apparatus, until mycelium pellet therein is all broken up；
5) bacteria suspension is dispensed：
Gained bacteria suspension is dispensed to sterile square container, size 50cm × 25cm × 5cm, container must have logical
Gas element, and ensure the sterile air being passed through.The volume for adding in bacteria suspension is 2L.
6) quiescent culture：
Container is placed in gnotobasis and is left to ferment, cultivation temperature：22-24℃.It cultivates 5 days, is on bacteria suspension surface
One layer of mycelia film can be grown.
7) film is dragged for：
In an aseptic environment, the mycelia film for growing completion is pulled out with particular tools such as tweezers, for the specific production such as facial mask
The preparation of product.
8) continue to cultivate：
The bacteria suspension for having dragged for mycelia film continues quiescent culture, can grow the mycelia film of same performance after 5 days again, so anti-
Multiple, a container can produce 18 mycelia films using a collection of bacteria suspension.
Mycelia membrane fiber diameter obtained is only 1-100nm, can go deep into the most thin rill of skin, is bonded skin line completely
Reason.It is through specific cutting, you can for the making of facial mask.
In addition, through bacteriostatic experiment, it was demonstrated that the flexible material is to staphylococcus aureus, Escherichia coli, Candida albicans
Bacteriostasis rate has broad-spectrum antibacterial all more than 99%.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (9)
1. a kind of production method of the hypha,hyphae flexible material as facial mask cloth, which is characterized in that include the following steps：
1) expand numerous parent species：Choose the healthy and strong dense test tube stock of cleaning-less bacteria infection, mycelia, in aseptic inoculation box, 1 piece of picking
5mm × 5mm sizes and parent species without culture medium are inoculated on the test tube slant culture medium of sterilizing cooling, in constant incubator
It is cultivated；
2) liquid spawn is prepared：In gnotobasis, take 2-3 block 5mm × 5mm sizes and the numerous parent species of expansion without culture medium are inoculated with
In the fluid nutrient medium of 100mL sterilizing coolings, gas bath shaking table culture；
3) it is diluted with water：Sterile water is added in liquid spawn to be diluted；
4) mycelium pellet is broken up：Using magnetic stirring apparatus treatment liquid strain 30min or so, until mycelium pellet therein is all beaten
It dissipates；
5) bacteria suspension is dispensed：Gained bacteria suspension is dispensed to special container, container there must be ventilation component, and ensure to be passed through
Sterile air；The volume for adding in bacteria suspension is the 1/3-1/2 of overall vessel volume；
6) quiescent culture：Container is placed in quiescent culture in gnotobasis；
7) film is dragged for：The mycelia film for growing completion is pulled out and (needs sterile working), for the making of the flexible material products such as facial mask cloth；
8) continue to cultivate：The bacteria suspension for having dragged for mycelia film continues quiescent culture.
2. production method according to claim 1, which is characterized in that the strain being inoculated with described in the step 1) is load
Bacterium, condition of culture --- cultivation temperature：22-28 DEG C, 7-10 days mycelia of culture can cover with inclined-plane.
3. production method according to claim 1, which is characterized in that the culture item of liquid spawn in the step 2)
Part --- temperature：22-28 DEG C, rotating speed：200-220rpm；Culture can obtain within 7 days or so bacterium solution clear, bacterium thick flavor,
Liquid spawn full of mycelium pellet.
4. production method according to claim 1, which is characterized in that the thinner ratio of liquid spawn in the step 3)
Example --- the volume ratio of liquid spawn and sterile water is 1：3.
5. production method according to claim 1, which is characterized in that the condition of quiescent culture in the step 6) --- training
Support temperature：20-28℃；Culture can grow mycelia film in 4 days or so.
6. production method according to claim 1, which is characterized in that the condition of quiescent culture in the step 8) --- training
Support temperature：20-28℃；Continue to cultivate 4 days or so mycelia films rear and that step 6) can be grown, so repeatedly, a container utilizes
A collection of bacteria suspension can produce 10-20 piece mycelia films.
7. production method according to claim 1, which is characterized in that Mother culture based formulas described in step 1) is：" horse
Bell potato+cotton seed hulls " extracting solution 1.0L, wherein, potato 200.0g, cotton seed hulls 20.0g；Glucose 20.0g, agar 20.0g, pH
It is natural；After packing, 121 DEG C of high pressure steam sterilization 30min are cooled down spare.
8. production method according to claim 1, which is characterized in that the production method of fluid nutrient medium is such as in step 2)
Under：Stirring in 25.0g corn flour, 15.0g beancake powder cold water is not had into block, is heated to boiling, boils 10 minutes, be during which stirred continuously,
Then 20.0g sucrose, 2.0g potassium dihydrogen phosphates, 1.0g magnesium sulfate, vitamin B are added in11, moisturizing to 1.0L stirs and evenly mixs；
After packing, 121 DEG C of high pressure steam sterilization 30min are cooled down spare.
9. a kind of hypha,hyphae flexible material as facial mask cloth, which is characterized in that the flexible material passes through claim 1-8
Any one of them production method makes to obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810161733.1A CN108186373B (en) | 2018-02-27 | 2018-02-27 | Method for manufacturing fungus hypha flexible material used as mask cloth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810161733.1A CN108186373B (en) | 2018-02-27 | 2018-02-27 | Method for manufacturing fungus hypha flexible material used as mask cloth |
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| Publication Number | Publication Date |
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| CN108186373A true CN108186373A (en) | 2018-06-22 |
| CN108186373B CN108186373B (en) | 2020-12-11 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188801A (en) * | 1998-01-14 | 1998-07-29 | 李宝健 | Ganoderma lucidum 10 ton tank fermentation technology |
| CN102824300A (en) * | 2012-08-20 | 2012-12-19 | 湖北智慧果林业科技有限公司 | Kudzu root fiber mask paper and mask manufactured by same |
| CN105837311A (en) * | 2016-03-22 | 2016-08-10 | 中国农业大学烟台研究院 | Mother strain culture medium capable of preventing degeneration of agaricus bisporus strain and preparation method thereof |
| CN106265474A (en) * | 2016-08-31 | 2017-01-04 | 百朗德生物化学(海门)有限公司 | A kind of method utilizing microbial strains fermenting and producing facial film |
-
2018
- 2018-02-27 CN CN201810161733.1A patent/CN108186373B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188801A (en) * | 1998-01-14 | 1998-07-29 | 李宝健 | Ganoderma lucidum 10 ton tank fermentation technology |
| CN102824300A (en) * | 2012-08-20 | 2012-12-19 | 湖北智慧果林业科技有限公司 | Kudzu root fiber mask paper and mask manufactured by same |
| CN105837311A (en) * | 2016-03-22 | 2016-08-10 | 中国农业大学烟台研究院 | Mother strain culture medium capable of preventing degeneration of agaricus bisporus strain and preparation method thereof |
| CN106265474A (en) * | 2016-08-31 | 2017-01-04 | 百朗德生物化学(海门)有限公司 | A kind of method utilizing microbial strains fermenting and producing facial film |
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| Publication number | Publication date |
|---|---|
| CN108186373B (en) | 2020-12-11 |
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