CN108169418A - The effect of sunscreen product property evaluation method - Google Patents
The effect of sunscreen product property evaluation method Download PDFInfo
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- CN108169418A CN108169418A CN201711342756.4A CN201711342756A CN108169418A CN 108169418 A CN108169418 A CN 108169418A CN 201711342756 A CN201711342756 A CN 201711342756A CN 108169418 A CN108169418 A CN 108169418A
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Abstract
The present invention relates to a kind of sunscreen product Efficiency assessment methods, include the following steps：Culturing liquid is obtained, sunscreen product and biosurfactant to be measured are added in into the culturing liquid, obtains sample solution, the active ingredient of the sunscreen product to be measured is alligator oil；Zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T, and ultraviolet irradiation is carried out to the zebra fish juvenile fish；The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, records the degree of injury of the zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.Sunscreen product Efficiency assessment method of the present invention using zebra fish as model organism, carries out Efficiency assessment to sunscreen product of the active ingredient for alligator oil, avoids and be detached from the Health cost of living organism and human test's method middle-ultraviolet lamp exit dose to people.
Description
Technical field
The present invention relates to cosmetic field, more particularly to sun care preparations the effect of property evaluation method.
Background technology
With the raising of people's living standard, the change of life idea, people's out on tours vacation tourism activity increases
Add, the time that skin is exposed to ultraviolet light is made constantly to increase, and Ozone in Atmosphere damage layer aggravates, and is radiated on human skin
Ultraviolet light is more and more.Therefore, be in great demand sun care preparations on the market, and the active ingredient in sun care preparations is sun-screening agent, including
Physical barrier agent and chemical absorbent.Physical opacifier is typically some lighttight substances, can reflect and scatter all
Ultraviolet light and visible ray, and the effect of chemical absorbent is selectively to absorb ultraviolet light.Cosmetics added with sun-screening agent
Smearing form layer protecting film on the skin, by absorb and reflect the ultraviolet light in sunlight avoid or postpone skin by
The injury of ultraviolet light, confrontation sunburn, blackening and skin light aging etc..
There are many sunscreen product type in the market, therefore, how a sunscreen product the effect of property of effectively evaluating, be convenient for
Consumer reasonably selects, and is the emphasis of people's research.
At present, the ultraviolet radiation protective effect detection of sun care preparations ingredient mainly has SPF vitro assays and human test
Method.SPF vitro assays are to cover UVB and UVA sections using artificial light source, are calculated by computer processing as a result, suitable
For researching and developing new formula spf value assessment and the quality control of production process.And human test's method is to carry out spf value by human body method
Measure it is obtained the result is that skin comprehensive erythema effect under the effect of certain length ultraviolet line, it is included simultaneously by ultraviolet
After the ultra-violet absorber degradation of line irradiation, the generated stimulating effect after skin surface and infiltration skin.It is but because each
Ultra violet radiation amount caused by kind of UVA, UVB light source is unknown to the Health cost consequence of people, thus human test's method exist compared with
Scruple in terms of big ethics.
Invention content
Based on this, it is necessary in view of the above-mentioned problems, providing the sunscreen product that a kind of operation is simplified, the period is short, reliability is high
Efficiency assessment method.
The present invention provides a kind of sunscreen product the effect of property evaluation method, includes the following steps：
Culturing liquid is obtained, sunscreen product and biosurfactant to be measured are added in into the culturing liquid, obtains sample solution,
The active ingredient of the sunscreen product to be measured is alligator oil；
Zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T, to institute
It states zebra fish juvenile fish and carries out ultraviolet irradiation；
The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, records the zebra fish children
The degree of injury of fish belly fin, tail fin and dorsal fin.
The biosurfactant is rhamnolipid in one of the embodiments,.
The mass ratio of the sunscreen product to be measured and the biosurfactant is 20- in one of the embodiments,
100:1。
In one of the embodiments, during the ultraviolet irradiation, at interval of 20-30min, 20-30s is irradiated, irradiates number
It is 6-10 times.
In one of the embodiments, during the ultraviolet irradiation, the distance of the transmitting terminal of ultraviolet light and the sample solution
For 5-20cm.
In one of the embodiments, during the ultraviolet irradiation, the wave-length coverage ultraviolet range of ultraviolet light exists
320-400nm。
It further includes in one of the embodiments,：
Blank control group is set：The culturing liquid is obtained, biosurfactant is added in into the culturing liquid, obtains blank
The sample solution of control group；
Zebra fish juvenile fish is cultivated in the sample solution of the blank control group, the time of the culture is T；
The zebra fish juvenile fish is transferred in the culturing liquid and is cultivated, record zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin
Degree of injury.
It further includes in one of the embodiments,：
Model control group is set：The culturing liquid is obtained, biosurfactant is added in into the culturing liquid, obtains model
The sample solution of control group；
Zebra fish juvenile fish is cultivated in the sample solution of the model control group, the time of the culture is T, and described
In time T, ultraviolet irradiation is carried out to the zebra fish juvenile fish；
The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, records zebra fish juvenile fish abdomen
The degree of injury of fin, tail fin and dorsal fin.
The acquisition methods of the zebra fish juvenile fish are in one of the embodiments,：
Sexually matured zebra fish is pressed into sex ration 1:2 pairings, after natural mating, collect fertilized eggs culture, select normal hair
Fish raise an infant as the zebra fish juvenile fish.
The principle of the present invention is as follows：
Zebra fish is a kind of low profile thermal hairtail, due to the advantages that egg laying amount is big, reproduction speed is fast, raising is simple, into
Animal is tested in recommendation for some toxicity standards (such as iso standard).Zebra fish is up to 87% with human gene similarity, this meaning
The two in gene expression and protein function have similitude, and many organs of zebra fish and the early development of system all with people
Class is very much like, therefore the evaluation result done experiment on zebra fish is also applied for human body in most cases, so handle
The evaluation result reliability higher of the zebra fish test animal both effectiveness as sunscreen product.
Active ingredient is alligator oil in sunscreen product to be measured, and alligator oil is rich in monounsaturated fatty acids and how unsaturated fat
Fat acid, in hydrophobicity, is not easy to disperse in zebra fish culturing liquid.Biosurfactant is one kind by plant, animal and micro- life
What object generated naturally has hydrophilic, lipophilic amphiphilic character substance, and good affinity is shown to oil-water interface, can shape
Into stable emulsion.On the one hand it can promote dispersion of the alligator oil in culturing liquid；On the other hand, with chemical surfactant phase
Than having the characteristics that nontoxic, harmless, not influencing the normal culture of zebra fish.
Compared with existing scheme, the invention has the advantages that：
Sunscreen product Efficiency assessment method of the present invention is alligator oil to active ingredient using zebra fish as model organism
Sunscreen product carry out Efficiency assessment, avoid and be detached from living organism and human test's method middle-ultraviolet lamp exit dose people is good for
Health is damaged.Since in actual use, sunscreen product to be measured generally stays in the surface of skin, and active ingredient alligator oil is water-soluble
Property it is bad, cause sunscreen product to be measured that can not be uniformly dispersed in culturing liquid, in turn result in zebra fish surface wrap up it is uneven.Cause
This, in evaluation procedure, adds in biosurfactant in culturing liquid, sunscreen product to be measured is enable to be uniformly dispersed in foster
It grows in liquid.
Preferred biosurfactant is rhamnolipid, and rhamnolipid molecule has polar hydrophilic group and nonpolarity parent
Oily unit structure typically exhibits the preferential distribution capability of very high surface-active and interface, enables sunscreen product to be measured uniform
Long-time be dispersed in culturing liquid.
In order to preferably simulate the effect of sun care preparations to be measured is to human skin, the sunscreen product to be measured and institute are adjusted
The mass ratio for stating biosurfactant is 20-100:1.Sunscreen product to be measured can be better achieved to wrap up zebra fish surface.
The present invention is prevented using suitable ultraviolet irradiation method processing zebra fish juvenile fish, the more scientific zebra fish evaluation that establishes
Shine the system of product.It can achieve the purpose that simplified operation, shortening period, quickening evaluation speed, reliability are high.
Description of the drawings
Fig. 1 is 0day zebra fish fin damage figure after ultraviolet irradiation；
Fig. 2 is 1day zebra fish fin damage figure after ultraviolet irradiation；
Fig. 3 is 2day zebra fish fin damage figure after ultraviolet irradiation；
Specific embodiment
The sunscreen product Efficiency assessment of the present invention is described in further detail below in conjunction with specific embodiment.
Embodiment 1
The present embodiment provides a kind of sunscreen product the effect of property evaluation methods, include the following steps,
(1) culturing liquid is obtained, sunscreen product and rhamnolipid to be measured are added in into the culturing liquid, obtains sample solution, institute
The active ingredient of sunscreen product to be measured is stated as alligator oil, a concentration of 0.5mg/ml of sunscreen product to be measured, sunscreen product to be measured with
The mass ratio of rhamnolipid is 50:1.
The ingredient of the culturing liquid includes：Sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.
(2) zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T,
Ultraviolet irradiation is carried out to the zebra fish juvenile fish；
The acquisition methods of zebra fish juvenile fish are：By sexal maturity zebra fish, (AB systems, national zebra fish resource center introduce, originally
Laboratory is expanded numerous) male and female divide cylinder, it raises in zebra fish culture unit.Zebra fish cultivation water (PH 6.7；520 μ s/cm of conductivity),
Water temperature is maintained at (26 ± 2) DEG C, and illumination/dark cycle control is in 14h:10h.Exposure experiment starts the previous day by male and female with 1:2
Pairing, natural mating oviposition.After natural mating, fertilized eggs culture is collected, selects the juvenile fish of 72hpf of normal development as this reality
Apply the zebra fish juvenile fish of example.
Zebra fish juvenile fish is placed in 6 orifice plates, 1/hole, 3ml sample solutions are added in per hole, two multiple holes are set.
The method of ultraviolet irradiation is：Irradiate 30s every 30min, irradiate 6 times, ultraviolet range in 320-400nm,
The transmitting terminal of ultraviolet light and the distance of sample solution are 5cm.
(3) the zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and cultivated, after ultraviolet irradiation, note
It records under stereomicroscope, when 0day, 1day, 2day, the degree of injury of zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.
The standard for judging zebra fish degree of injury is：Integrality >=90% of tail fin is normal, the integrality 20%- of tail fin
90% is reduces, and the integrality ＜ 20% of tail fin is missing.
(4) blank control group is set：
The culturing liquid is obtained, rhamnolipid is added in into the culturing liquid, obtains the sample solution of blank control group；
Zebra fish juvenile fish is cultivated in the sample solution of the blank control group, the time of the culture is T；
Divide sexal maturity zebra fish (AB systems, national zebra fish resource center introduce, this laboratory is expanded numerous) male and female to cylinder, raise
In zebra fish culture unit.Zebra fish cultivation water (PH 6.7；520 μ s/cm of conductivity), water temperature is maintained at (26 ± 2) DEG C, light
According to the control of/dark cycle in 14h:10h.Exposure experiment starts the previous day by male and female with 1:2 pairings, natural mating oviposition.It is natural
Post-coitum collects fertilized eggs culture, selects zebra fish juvenile fish of the juvenile fish as the present embodiment of the 72hpf of normal development.
Zebra fish juvenile fish is placed in 6 orifice plates, 1/hole, 3ml sample solutions are added in per hole, two multiple holes are set.
The zebra fish juvenile fish is transferred in the culturing liquid and is cultivated, is recorded under stereomicroscope, 0day, 1day,
During 2day, the degree of injury of zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.
(5) model control group is set：
The culturing liquid is obtained, rhamnolipid is added in into the culturing liquid, obtains the sample solution of model control group；
Zebra fish juvenile fish is cultivated in the sample solution of the model control group, the time of the culture is T, and described
In time T, ultraviolet irradiation is carried out to the zebra fish juvenile fish；
Divide sexal maturity zebra fish (AB systems, national zebra fish resource center introduce, this laboratory is expanded numerous) male and female to cylinder, raise
In zebra fish culture unit.Zebra fish cultivation water (PH 6.7；520 μ s/cm of conductivity), water temperature is maintained at (26 ± 2) DEG C, light
According to the control of/dark cycle in 14h:10h.Exposure experiment starts the previous day by male and female with 1:2 pairings, natural mating oviposition.It is natural
Post-coitum collects fertilized eggs culture, selects zebra fish juvenile fish of the juvenile fish as the present embodiment of the 72hpf of normal development.
Zebra fish juvenile fish is placed in 6 orifice plates, 1/hole, 3ml sample solutions are added in per hole, two multiple holes are set.
The method of ultraviolet irradiation is：Irradiate 30s every 30min, irradiate 6 times, ultraviolet range in 320-400nm,
The transmitting terminal of ultraviolet light and the distance of sample solution are 5cm.
The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, records stereomicroscope
Under, when 0day, 1day, 2day, the degree of injury of zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.
Embodiment 2
The present embodiment provides a kind of sunscreen product the effect of property evaluation methods, include the following steps,
(1) culturing liquid is obtained, sunscreen product and rhamnolipid to be measured are added in into the culturing liquid, obtains sample solution, institute
The active ingredient for stating sunscreen product to be measured is alligator oil, a concentration of 1mg/ml of sunscreen product to be measured, sunscreen product to be measured and mouse
The mass ratio of Lee's glycolipid is 50:1.
The ingredient of the culturing liquid includes：Sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.
(2) zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T,
Ultraviolet irradiation is carried out to the zebra fish juvenile fish；
Divide sexal maturity zebra fish (AB systems, national zebra fish resource center introduce, this laboratory is expanded numerous) male and female to cylinder, raise
In zebra fish culture unit.Zebra fish cultivation water (PH 6.7；520 μ s/cm of conductivity), water temperature is maintained at (26 ± 2) DEG C, light
According to the control of/dark cycle in 14h:10h.Exposure experiment starts the previous day by male and female with 1:2 pairings, natural mating oviposition.It is natural
Post-coitum collects fertilized eggs culture, selects zebra fish juvenile fish of the juvenile fish as the present embodiment of the 72hpf of normal development.
Zebra fish juvenile fish is placed in 6 orifice plates, 1/hole, 3ml sample solutions are added in per hole, two multiple holes are set.
The method of ultraviolet irradiation is：Irradiate 30s every 30min, irradiate 6 times, ultraviolet range in 320-400nm,
The transmitting terminal of ultraviolet light and the distance of sample solution are 5cm.
(3) the zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and cultivated, after ultraviolet irradiation, note
It records under stereomicroscope, when 0day, 1day, 2day, the degree of injury of zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.
Judge that the standard that zebra fish damages is：Integrality >=90% of tail fin is normal, the integrality 20%-90% of tail fin
To reduce, the integrality ＜ 20% of tail fin is missing.
The blank control group and model control group of the present embodiment are same as Example 1.
Embodiment 3
The present embodiment provides a kind of sunscreen product the effect of property evaluation methods, include the following steps,
(1) culturing liquid is obtained, sunscreen product and rhamnolipid to be measured are added in into the culturing liquid, obtains sample solution, institute
The active ingredient for stating sunscreen product to be measured is alligator oil, a concentration of 2mg/ml of sunscreen product to be measured, sunscreen product to be measured and mouse
The mass ratio of Lee's glycolipid is 50:1.
The ingredient of the culturing liquid includes：Sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.
(2) zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T,
Ultraviolet irradiation is carried out to the zebra fish juvenile fish；
Divide sexal maturity zebra fish (AB systems, national zebra fish resource center introduce, this laboratory is expanded numerous) male and female to cylinder, raise
In zebra fish culture unit.Zebra fish cultivation water (PH 6.7；520 μ s/cm of conductivity), water temperature is maintained at (26 ± 2) DEG C, light
According to the control of/dark cycle in 14h:10h.Exposure experiment starts the previous day by male and female with 1:2 pairings, natural mating oviposition.It is natural
Post-coitum collects fertilized eggs culture, selects zebra fish juvenile fish of the juvenile fish as the present embodiment of the 72hpf of normal development.
Zebra fish juvenile fish is placed in 6 orifice plates, 1/hole, 3ml sample solutions are added in per hole, two multiple holes are set.
The method of ultraviolet irradiation is：Irradiate 30s every 30min, irradiate 6 times, ultraviolet range in 320-400nm,
The transmitting terminal of ultraviolet light and the distance of sample solution are 5cm.
(3) the zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and cultivated, after ultraviolet irradiation, note
It records under stereomicroscope, when 0day, 1day, 2day, the degree of injury of zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin.
Judge that the standard that zebra fish damages is：Integrality >=90% of tail fin is normal, the integrality 20%-90% of tail fin
To reduce, the integrality ＜ 20% of tail fin is missing.
The blank control group and model control group of the present embodiment are same as Example 1.
Comparative example 1
This comparative example provides a kind of sunscreen product the effect of property evaluation method, substantially the same manner as Example 3, difference lies in,
Rhamnolipid is not added in.
Comparative example 2
This comparative example provides a kind of sunscreen product the effect of property evaluation method, substantially the same manner as Example 3, difference lies in,
Add in dodecyl methanesulfonic acid.
Embodiment 1-3 and the zebra fish fin area result of comparative example 1,2 are as shown in table 1；The fish of embodiment 1-3 zebra fish
Fin changes as shown in Figs. 1-3.
1 zebra fish fin area (mm of table2)
Blank control group the result shows that, under the normal breeding condition without ultraviolet irradiation, zebra fish in certain time
Fin area increases with the extension for cultivating the time.
Model control group interpretation of result：As shown in Table 1, after ultraviolet irradiation during 0day, zebra fish and the sky of model control group
The fin area of white control group zebra fish has no too big difference, after ultraviolet irradiation when 1day, 2day, the zebra fish of model control group
Fin area significantly reduce compared with the fin area of blank control group；It can be obtained by Fig. 1, the zebra fish pair with blank control group
Than after ultraviolet irradiation during 0day, the integrality of zebra fish juvenile fish tail fin is 96.54%, is excluded for normal zebra fish juvenile fish
The influence of experimental subjects notable difference.And after ultraviolet irradiation when 1day, 2day, with reference to Fig. 2 and Fig. 3, zebra fish abdomeinal fin, tail fin
With dorsal fin occur major injury, compare with the zebra fish of blank control group, ultraviolet light irradiate after 1day, 2day when, tail fin it is complete
Whole property is respectively 35.82%, 4.83%, shows as reducing, lack.Fig. 3 shows that 2day after ultraviolet irradiation, tail fin is lacked to be serious
It loses.Illustrate that ultraviolet light irradiation has larger damaging action to zebra fish fin.
In embodiment 1, the sunscreen product to be measured of a concentration of 0.5mg/ml, as shown in Table 1,0day after ultraviolet irradiation are added in
When, zebra fish fin area is with blank control group zebra fish fin area without too big difference.After ultraviolet irradiation when 1day, 2day,
The area of fin reduces, and is compared with the fin area of blank control group and the zebra fish of model control group it is found that adding in dense
It spends for after 0.5mg/ml sunscreen products to be measured, zebra fish fin area is increased compared with the fin area of model control group；Fig. 1
In, it after ultraviolet irradiation during 0day, is compareed with the zebra fish of blank control group, the integrality of tail fin is 98.08%, and performance is just
Often, the influence of experimental subjects notable difference is excluded for normal zebra fish juvenile fish.And after ultraviolet irradiation when 1day, 2day, with reference to
Fig. 2 and Fig. 3, zebra fish abdomeinal fin, tail fin and dorsal fin generation are damaged, are compareed with the zebra fish of blank control group, tail fin by a small margin
Integrality be respectively 35.82%, 4.83%, show as reducing.Fig. 3 is shown, after ultraviolet irradiation 2day, tail fin degree of injury
Weaken.Illustrate that a concentration of 0.5mg/ml sunscreen products to be measured have protective effect to zebra fish fin.
In embodiment 2, after adding in the sunscreen product to be measured of a concentration of 1mg/ml, as shown in Table 1,0day after ultraviolet irradiation
When, zebra fish fin area is with blank control group zebra fish fin area without too big difference.After ultraviolet irradiation when 1day, 2day,
The area of fin slightly reduces, be compared with the fin area of blank control group and the zebra fish of model control group it is found that plus
After entering a concentration of 1mg/ml sunscreen products to be measured, zebra fish fin area has compared with the fin area of model control group significantly to be increased
Greatly, it in Fig. 1, is compareed with the zebra fish of blank control group, the integrality of tail fin is 96.15%, is acted normally, and is normal zebra
Fish juvenile fish excludes the influence of experimental subjects notable difference.And after ultraviolet irradiation when 1day, 2day, with reference to Fig. 2 and Fig. 3, zebra
Slight damage occurs for fish belly fin, tail fin and dorsal fin, is compareed with the zebra fish of blank control group, the integrality of tail fin is respectively
81.95%th, 89.61%, it shows as reducing.Fig. 3 is shown, after ultraviolet irradiation 2day, tail fin degree of injury significantly weakens.Explanation
A concentration of 1mg/ml sunscreen products to be measured have more obvious protective effect to zebra fish fin.
In embodiment 3, after adding in the sunscreen product to be measured of a concentration of 2mg/ml, in table 1, the area of fin becomes without apparent
Change, be compared with the fin area of blank control group and the zebra fish of model control group it is found that adding in a concentration of 2mg/ml and treated
After surveying sunscreen product, the fin area of zebra fish fin area and blank control group does not have apparent difference, can be considered fin face
Product is not reduced, and fin is not by ultraviolet injury.It in Fig. 1, is compareed with the zebra fish of blank control group, the integrality of tail fin
It is 96.15%, acts normally, the influence of experimental subjects notable difference is excluded for normal zebra fish juvenile fish.After ultraviolet irradiation
When 1day, 2day, with reference to Fig. 2 and Fig. 3, zebra fish abdomeinal fin, tail fin and dorsal fin do not damage, the zebra fish with blank control group
Control, the integrality of tail fin is respectively 97.71%, 98.31%, is shown as complete.Illustrate that a concentration of 2mg/ml is to be measured sun-proof
Product has the function of fully against ultraviolet light.
In comparative example 1, biosurfactant is not added in, and active ingredient is the sunscreen product of alligator oil in sample solution
Disperse uneven, part zebra fish is not affected by adequately protecting for sunscreen product, makes test result inaccurate.
In comparative example 2, using dodecyl methanesulfonic acid, the dispersion effect of sunscreen product is not as good as adding in rhamnolipid.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
1. the effect of a kind of sunscreen product property evaluation method, which is characterized in that include the following steps：
Culturing liquid is obtained, sunscreen product and biosurfactant to be measured are added in into the culturing liquid, obtains sample solution, it is described
The active ingredient of sunscreen product to be measured is alligator oil；
Zebra fish juvenile fish is cultivated in the sample solution, the time of the culture is T, and in the time T, to the spot
Horse fish juvenile fish carries out ultraviolet irradiation；
The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, records the zebra fish juvenile fish abdomen
The degree of injury of fin, tail fin and dorsal fin.
2. the effect of sunscreen product according to claim 1 property evaluation method, which is characterized in that the Bio-surface active
Agent is rhamnolipid.
3. the effect of sunscreen product according to claim 1 property evaluation method, which is characterized in that the sunscreen product to be measured
Mass ratio with the biosurfactant is 20-100:1.
4. according to claim 1-3 any one of them sunscreen product the effect of property evaluation methods, which is characterized in that described ultraviolet
The technological parameter of irradiation is：At interval of 20-30min, 20-30s is irradiated, irradiation number is 6-10 times.
5. the effect of sunscreen product according to claim 4 property evaluation method, which is characterized in that during the ultraviolet irradiation,
The distance of the transmitting terminal of ultraviolet light and the sample solution is 5-20cm.
6. the effect of sunscreen product according to claim 4 property evaluation method, which is characterized in that during the ultraviolet irradiation,
Ultraviolet range is in 320-400nm.
7. the effect of sunscreen product according to claim 1 property evaluation method, which is characterized in that further include：
Blank control group is set：The culturing liquid is obtained, biosurfactant is added in into the culturing liquid, obtains blank control
The sample solution of group；
Zebra fish juvenile fish is cultivated in the sample solution of the blank control group, the time of the culture is T；
The zebra fish juvenile fish is transferred in the culturing liquid and is cultivated, the damage of record zebra fish juvenile fish abdomeinal fin, tail fin and dorsal fin
Hinder degree.
8. the effect of sunscreen product according to claim 1 property evaluation method, which is characterized in that further include：
Model control group is set：The culturing liquid is obtained, biosurfactant is added in into the culturing liquid, obtains model comparison
The sample solution of group；
Zebra fish juvenile fish is cultivated in the sample solution of the model control group, the time of the culture is T, and in the time
In T, ultraviolet irradiation is carried out to the zebra fish juvenile fish；
The zebra fish juvenile fish after ultraviolet irradiation is transferred in the culturing liquid and is cultivated, record zebra fish juvenile fish abdomeinal fin,
The degree of injury of tail fin and dorsal fin.
9. the effect of sunscreen product according to claim 1 property evaluation method, which is characterized in that the zebra fish juvenile fish
Acquisition methods are：
Sexually matured zebra fish is pressed into sex ration 1:2 pairings, after natural mating, collect fertilized eggs culture, select normal development children
Fish is as the zebra fish juvenile fish.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108956606A (en) * | 2018-07-09 | 2018-12-07 | 广东蓝堡生物科技有限公司 | A kind of detection method of sunlight screening skin-protecting product sun-proof function |
| CN112198143A (en) * | 2020-09-04 | 2021-01-08 | 南京新环检测科技有限公司 | Method for evaluating anti-photoaging and glycosylation effects of cosmetics and health-care foods |
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| CN112544571A (en) * | 2020-12-03 | 2021-03-26 | 上海应用技术大学 | Establishment and application of caenorhabditis elegans model for evaluating safety and efficacy of sun-screening agent |
| CN112544571B (en) * | 2020-12-03 | 2023-03-03 | 上海应用技术大学 | Establishment and application of caenorhabditis elegans model for evaluating safety and efficacy of sun-screening agent |
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