CN108956606A - A kind of detection method of sunlight screening skin-protecting product sun-proof function - Google Patents
A kind of detection method of sunlight screening skin-protecting product sun-proof function Download PDFInfo
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- CN108956606A CN108956606A CN201810747616.3A CN201810747616A CN108956606A CN 108956606 A CN108956606 A CN 108956606A CN 201810747616 A CN201810747616 A CN 201810747616A CN 108956606 A CN108956606 A CN 108956606A
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- zebra fish
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- protecting product
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The present invention relates to detection technique fields, and in particular to a kind of detection method of sunlight screening skin-protecting product sun-proof function；The present invention evaluates the sun-proof function of sun-proof class skin care item by comparison ultraviolet light irradiation front and back zebra fish fin integrality using zebra fish as experimental vehicle；Experimental period of the invention is short, low in cost, and the scientific and effective sun-proof function to sun-proof class skin care item of energy is evaluated.
Description
Technical field
The present invention relates to detection technique fields, and in particular to a kind of detection method of sunlight screening skin-protecting product sun-proof function.
Background technique
With the continuous promotion of people 's material life level, the change of philosophy of life goes out work, tourism, shopping etc. outside
Skin can be exposed under ultraviolet radiation for a long time when movable.For reach prevent skin by it is tanned, sunburn the purpose of and adopt
Certain methods are taken to obstruct or absorb ultraviolet light.General sun-proof method: sunscreen product is smeared, using sunproof clothes and other items, food
With sun-proof food etc..Succinctly facilitate due to smearing sun-proof class skin care item, more and more people are outgoing to rub sun-proof class shield
Skin product.
The effect of sunlight screening skin-protecting product, depends primarily on SPF the and PA value of sunlight screening skin-protecting product, and SPF index is high, ultraviolet protection
Time is with regard to president.The sun-proof function of traditional sun-proof class skin care item is mainly tested with mouse, monkey even human body, these
The experimental period of experimental method is long, costly, is not suitable for the screening of a large amount of allogenic materials.
Summary of the invention
To solve the above-mentioned problems, the object of the invention is that, a kind of detection method of sunlight screening skin-protecting product sun-proof function is provided；
This method can quickly, and the scientific and effective sun-proof function to sun-proof class skin care item is evaluated.
The present invention is achieved through the following technical solutions:
A kind of detection method of sunlight screening skin-protecting product sun-proof function, method particularly includes:
(1) sunlight screening skin-protecting product to be detected is dissolved into the cultivation water containing zebra fish, the sun-proof shield to be detected
Concentration of the skin product in cultivation water is 400-800mg/L；
(2) it by the cultivation water containing sunlight screening skin-protecting product to be detected, zebra fish, is irradiated in ultraviolet light, to contain zebra fish
Cultivation water group without ultraviolet light irradiation be blank control (- UV), the cultivation water group containing zebra fish by ultraviolet light irradiation
For model comparison (+UV)；
(3) zebra fish that each group in step (2) passes through ultraviolet light irradiation is taken out, bat takes zebra fish yolk bag down toward tail portion
Photo, focus on zebra fish fin position, measure zebra fish fin area；
(4) zebra fish fin face in the zebra fish fin and-UV and+UV in sunlight screening skin-protecting product cultivation water is contained by comparison
Long-pending size detects the sun-proof result of the sunlight screening skin-protecting product.
The detection method of the sunlight screening skin-protecting product sun-proof function, method particularly includes:
(1) prepare:
Before experiment, a pair of of male and female zebra fish is selected in mating box, is separated zebra fish male and female with partition, night or dark shape
State places 10h；The next morning pumping opens partition, zebra fish mate and oviposit；It pulls zebra fish out, collects zebra fish fish-egg；
(2) the partition time is opened as 0hpf (hours post-fertilization) to take out, start to test in 72hpf；
(3) specific experiment:
A. experimental solutions are prepared, with cultivation water group without ultraviolet light irradiation for-UV, ultraviolet light is passed through with cultivation water group
Irradiation is+UV；
B. zebra fish is loaded with glass dish, is cleaned 2-3 times with cultivation water or PBS, every zebra fish is numbered, body is placed in
It takes pictures under formula microscope；Calibrating length is carried out to Stereo microscope, is taken pictures under 5 times of mirrors of Stereo microscope, bat takes zebra
Fish yolk bag corresponds to focus on zebra fish fin position down toward the photo of tail portion and has recorded number, be experiment with 6 orifice plates
Experimental solutions 5ml is added in container, 1, every hole zebra fish；
C. by the 6 orifice plates that experimental solutions and zebra fish is added be placed in CL-1000 UV crosslinking instrument (wavelength 302nm and
It is irradiated under 365nm), irradiates 30s, concurrent irradiation 5-6 times every time；
D. after irradiating, zebra fish is sucked out from 6 orifice plates with plastic suction pipe, is cleaned 1-2 times with cultivation water.Then by spot
Horse fish is transferred to 24 orifice plates, corresponds to and has recorded zebra fish number；24 orifice plates are placed in biochemical cultivation case and are cultivated；
E. it takes pictures under 5 times of mirrors of Stereo microscope when 24,48h after ultraviolet light irradiation, bat takes zebra fish yolk bag
To focus on zebra fish fin position, the number of recording photograph and corresponding zebra fish down toward the photo of tail portion；Export picture extremely
Computer measures zebra fish fin area using Image；
F. zebra fish fin face in the zebra fish fin and-UV and+UV in sunlight screening skin-protecting product cultivation water is contained by comparison
Long-pending size detects the sun-proof result of the sunlight screening skin-protecting product.
Preferably, the male and female zebra fish selected is wild type AB system zebra fish.
Preferably, in (1) before experiment, 22:00 or so every night selects a pair of of male and female zebra fish in mating box, with every
Plate separates zebra fish male and female, and night or dark state place 10h；The next morning 8:00 or so pumping opens partition, and zebra fish is handed over
With oviposition；It pulls zebra fish out, collects zebra fish fish-egg.
Preferably, 24 orifice plate is placed in biochemical cultivation case, incubator temperature is 28 ± 0.5 DEG C.
The present invention is using zebra fish as experimental vehicle, by zebra fish fin integrality to the sun-proof function of sun-proof class skin care item
It is evaluated.By long-time scientific validation, model organism zebra fish can solve these problems very well.Zebra fish individual is small, easily
Raising, breeding is fast, and experimental period is short, low in cost, reaches 87% with human gene homology.Therefore can quickly, and science has
Effect evaluates the sun-proof function of sun-proof class skin care item.Zebra fish fin is transparent, and very sensitive to extraneous environmental stimulus,
Respective reaction can be made after by ultraviolet radiation.Therefore zebra fish can be very good the sun-proof function to sun-proof class skin care item
It is evaluated.
Detailed description of the invention
Fig. 1 is the present invention with the Octocrilene of various concentration, before ultraviolet light irradiation, 1 day, 2 days zebra fish fins after irradiation
Area control；Using the cultivation water group that no ultraviolet light irradiates as-UV, using the cultivation water group by ultraviolet light irradiation as+UV.
Fig. 2 is the present invention with 1 day, 2 days zebra fish fin relative areas after the Octocrilene ultraviolet light irradiation of various concentration
Control；Using the cultivation water group that no ultraviolet light irradiates as-UV, using the cultivation water group by ultraviolet light irradiation as+UV.
Fig. 3 is zebra fish fin photo before the present invention is irradiated with the Octocrilene ultraviolet light of various concentration；With no ultraviolet light
The cultivation water group conduct-UV of irradiation, using the cultivation water group by ultraviolet light irradiation as+UV.
Fig. 4 is the present invention with 1 day zebra fish fin photo after the Octocrilene ultraviolet light irradiation of various concentration；Without ultraviolet
The cultivation water group conduct-UV of line irradiation, using the cultivation water group by ultraviolet light irradiation as+UV.
Fig. 5 is the present invention with 2 days zebra fish fin photos after the Octocrilene ultraviolet light irradiation of various concentration；Without ultraviolet
The cultivation water group conduct-UV of line irradiation, using the cultivation water group by ultraviolet light irradiation as+UV.
Specific embodiment
A kind of detection method of sunlight screening skin-protecting product sun-proof function, specific experiment method are as follows:
(1) test before (probably 22:00 every night), select a pair of of male and female (wild type AB system) zebra fish in mate box, with every
Plate separates zebra fish male and female, and night (dark) state places 10h.
(2) the next morning (8:00 or so) takes out and opens partition, zebra fish mate and oviposit.
(3) it pulls zebra fish out, collects zebra fish fish-egg.
(4) the partition time is opened as 0hpf to take out, start to test in 72hpf.
(5) experimental solutions are prepared, with cultivation water group without ultraviolet light irradiation for-UV, with cultivation water group by ultraviolet
Line irradiation is+UV.
(6) zebra fish is loaded with glass dish, is cleaned 2~3 times with cultivation water (or PBS).Every zebra fish is numbered, is set
It takes pictures under Stereo microscope.
(7) calibrating length is carried out to Stereo microscope.It takes pictures under 5 times of mirrors of Stereo microscope, bat takes zebra fish-egg
Yellow capsule is to focus on zebra fish fin position down toward the photo of tail portion.Number is corresponded to and has been recorded, is experiment container with 6 orifice plates,
Experimental solutions 5ml is added in 1, every hole zebra fish.
(8) by the 6 orifice plates that experimental solutions and zebra fish is added be placed in CL-1000 UV crosslinking instrument (wavelength 302nm and
It is irradiated under 365nm), irradiates 30s every time, concurrent irradiation 5~6 times.
(9) after irradiating, zebra fish is sucked out from 6 orifice plates with plastic suction pipe, is cleaned 1~2 time with cultivation water.Then will
Zebra fish is transferred to 24 orifice plates, corresponds to and has recorded zebra fish number.
(10) 24 orifice plates are placed in biochemical cultivation case, are cultivated in 28 ± 0.5 DEG C.
(11) it takes pictures under 5 times of mirrors of Stereo microscope when 24,48h after ultraviolet light irradiation, bat takes zebra fish yolk
Capsule is to focus on zebra fish fin position down toward the photo of tail portion.The number of recording photograph and corresponding zebra fish.
(12) export picture measures zebra fish fin area using Image to computer.
- UV group, before ultraviolet light irradiation, development 24, the variation of 48h fin area are shown in Table 1.
Table 1
| Ultraviolet light radiation situation | -UV |
| Before ultraviolet light irradiation | 100.00% |
| Ultraviolet light irradiates for 24 hours | 134.20 ± 2.04% |
| Ultraviolet light irradiates 48h | 159.98 ± 5.87% |
+ UV group and drug concentration group, relative to the variation of-UV group fin area ratio, see Table 2 for details
Table 2
By upper 1 table 2 of table it follows that
(1)-UV group, zebra fish fin is complete, and the normal development as time goes by of fin area.
(2)+UV group, zebra fish fin have obvious damage compared with-UV.
(3) compared with-UV group, each concentration group of Octocrilene has obvious damage, compared with+UV group, zebra fish fin area
It damages smaller.And with the increase of concentration, and damaged area is smaller.
(4) compared according to the data of-UV ,+UV and medicine group, the sun-proof model of zebra fish is effective, model foundation success.
Above-described embodiment, only presently preferred embodiments of the present invention, is not intended to limit the invention practical range, therefore all with this
The equivalent change or modification that feature described in invention claim and principle are done should all be included in scope of the invention as claimed
Within.
Claims (5)
1. a kind of detection method of sunlight screening skin-protecting product sun-proof function, which is characterized in that method particularly includes:
(1) sunlight screening skin-protecting product to be detected is dissolved into the cultivation water containing zebra fish, the sunlight screening skin-protecting product to be detected
Concentration in cultivation water is 400-800mg/L；
(2) it by the cultivation water containing sunlight screening skin-protecting product to be detected, zebra fish, is irradiated in ultraviolet light, with supporting containing zebra fish
Growing water group without ultraviolet light irradiation is blank control-UV, and the cultivation water group containing zebra fish is model by ultraviolet light irradiation
Control+UV；
(3) zebra fish that each group in step (2) passes through ultraviolet light irradiation is taken out, bat takes zebra fish yolk bag down toward the photograph of tail portion
Piece focuses on zebra fish fin position, measures zebra fish fin area；
(4) zebra fish fin area in the zebra fish fin and-UV and+UV in sunlight screening skin-protecting product cultivation water is contained by comparison
Size detects the sun-proof result of the sunlight screening skin-protecting product.
2. the detection method of sunlight screening skin-protecting product sun-proof function as described in claim 1, which is characterized in that method particularly includes:
(1) prepare:
Before experiment, a pair of of male and female zebra fish is selected in mating box, is separated zebra fish male and female with partition, night or dark state are put
Set 10h；The next morning pumping opens partition, zebra fish mate and oviposit；It pulls zebra fish out, collects zebra fish fish-egg；
(2) the partition time is opened as 0hpf (hours post-fertilization) to take out, start to test in 72hpf；
(3) specific experiment:
A. experimental solutions are prepared, are irradiated with cultivation water group without ultraviolet light for blank control (- UV), with cultivation water group process
Ultraviolet light irradiation is model comparison (+UV)；
B. zebra fish is loaded with glass dish, is cleaned 2-3 times with cultivation water or PBS, every zebra fish is numbered, it is aobvious to be placed in body formula
It takes pictures under micro mirror；Calibrating length is carried out to Stereo microscope, is taken pictures under 5 times of mirrors of Stereo microscope, bat takes zebra fish-egg
Yellow capsule corresponds to focus on zebra fish fin position down toward the photo of tail portion and has recorded number, be experiment container with 6 orifice plates,
Experimental solutions 5ml is added in 1, every hole zebra fish；
C. the 6 orifice plates that experimental solutions and zebra fish are added are placed under CL-1000 UV crosslinking instrument (wavelength 302nm and 365nm)
Irradiation, irradiates 30s, concurrent irradiation 5-6 times every time；
D. after irradiating, zebra fish is sucked out from 6 orifice plates with plastic suction pipe, is cleaned 1-2 times with cultivation water.Then by zebra fish
24 orifice plates are transferred to, correspond to and have recorded zebra fish number；24 orifice plates are placed in biochemical cultivation case and are cultivated；
E when 24,48h, takes pictures after ultraviolet light irradiation under 5 times of mirrors of Stereo microscope, and bat takes zebra fish yolk bag or less
To the photo of tail portion, zebra fish fin position, the number of recording photograph and corresponding zebra fish are focused on；Picture is exported to electricity
Brain measures zebra fish fin area using Image；
F. zebra fish fin area in the zebra fish fin and-UV and+UV in sunlight screening skin-protecting product cultivation water is contained by comparison
Size detects the sun-proof result of the sunlight screening skin-protecting product.
3. the detection method of sunlight screening skin-protecting product sun-proof function as claimed in claim 2, which is characterized in that the male and female spot selected
Horse fish is wild type AB system zebra fish.
4. the detection method of sunlight screening skin-protecting product sun-proof function as claimed in claim 2, which is characterized in that in (1) before experiment,
22:00 every night selects a pair of of male and female zebra fish in mating box, is separated zebra fish male and female with partition, night or dark state are put
Set 10h；The next morning 8:00 pumping opens partition, zebra fish mate and oviposit；It pulls zebra fish out, collects zebra fish fish-egg.
5. the detection method of sunlight screening skin-protecting product sun-proof function as claimed in claim 2, which is characterized in that 24 orifice plate is placed in life
Change incubator, incubator temperature is 28 ± 0.5 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544571A (en) * | 2020-12-03 | 2021-03-26 | 上海应用技术大学 | Establishment and application of caenorhabditis elegans model for evaluating safety and efficacy of sun-screening agent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544571A (en) * | 2020-12-03 | 2021-03-26 | 上海应用技术大学 | Establishment and application of caenorhabditis elegans model for evaluating safety and efficacy of sun-screening agent |
| CN112544571B (en) * | 2020-12-03 | 2023-03-03 | 上海应用技术大学 | Establishment and application of caenorhabditis elegans model for evaluating safety and efficacy of sun-screening agent |
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