CN108160126B - Micro-fluidic chip for high-throughput enrichment of micro-particles - Google Patents
Micro-fluidic chip for high-throughput enrichment of micro-particles Download PDFInfo
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
Abstract
The invention discloses a micro-particle high-flux enrichment microfluidic chip which is sequentially stacked from top to bottom and provided with an enrichment module, an inner outlet collection module, a drainage module, an outer outlet collection module and a flow control module, wherein a sample inlet is formed in the center of the enrichment module, the sample inlet is communicated with an inertial concentration flow channel, the inertial concentration flow channel is communicated with a branched flow channel, the branched flow channel is communicated with an inner outlet and an outer outlet, a blank solution at the inner outlet of the enrichment module is collected to the middle by the inner outlet collection module, the blank solution collected by the inner outlet collection module is drained to the side edge by the drainage module, a concentrated solution at the outer outlet is collected to the middle by the outer outlet collection module, and the flow resistance of the blank solution and the concentrated solution is limited by the flow control module and then is led out. The invention has high concentration efficiency, can meet the enrichment requirement of sample liquid with extremely low concentration or large volume, has wide application range, simple chip manufacture, low cost and simple use and operation.
Description
Technical Field
The invention relates to an enrichment chip, in particular to a micro-particle high-flux enrichment microfluidic chip.
Background
Nowadays, the micro-particle enrichment technology is almost ubiquitous, and has become an important sample pretreatment link in numerous industries such as biological research, chemical analysis, environmental detection and medical diagnosis.
The main methods for particle enrichment are centrifugation and pore filtration. The centrifugation method mainly makes particles in the solution settled through high-speed rotation, and then the supernatant is poured out to obtain the enrichment solution. The method often requires the help of expensive instruments, so that the method cannot be used in the field or in a remote and poor area, and meanwhile, the high-speed centrifugation causes inevitable damage to a flexible biological sample with extremely dilute concentration. Compared with a high-speed centrifugation method, the principle of pore filtration is simpler, the main part of the method is a film containing a microporous structure with a specific size, the solid content in the sample can be blocked by placing the film at a filter opening, the pore diameter of the film is larger than that of the micropores, and blank liquid is filtered away to achieve the purpose of enrichment. Because the microporous membrane is easy to block, after a certain amount of solid components are accumulated, the sample liquid is difficult to flow out smoothly, so that the enrichment requirement of high-concentration or large-volume sample liquid cannot be met. Furthermore, the current approaches are still difficult to solve for the problem of how to remove the particles sticking to the film. The former is mainly used in laboratories and hospitals, and the latter is mostly used for obtaining clear liquid at present.
Disclosure of Invention
The purpose of the invention is as follows: the invention provides a micro-fluidic chip for high-throughput enrichment of micro-particles, which solves the problems that the existing micro-particle enrichment method is low in concentration efficiency, cannot meet the enrichment requirement of sample liquid with extremely low concentration or large volume, is narrow in application range and is inconvenient to operate.
The technical scheme is as follows: the micron particle high-flux enrichment microfluidic chip is sequentially provided with an enrichment module, an inner outlet collection module, a drainage module, an outer outlet collection module and a flow control module in a stacking mode from top to bottom, wherein a sample inlet is formed in the center of the enrichment module, the sample inlet is communicated with an inertial concentration flow channel, the inertial concentration flow channel is communicated with a branched flow channel, the branched flow channel is communicated with an inner outlet and an outer outlet, a blank solution at the inner outlet of the enrichment module is collected to the middle by the inner outlet collection module, the blank solution collected by the inner outlet collection module is drained to the side edge by the drainage module, a concentrated solution at the outer outlet is collected to the middle by the outer outlet collection module, and the flow resistance of the blank solution and the concentrated solution is limited by the flow control module and then is led out.
In order to improve the flux of the processed sample liquid per unit time, the inertial concentration flow channel is a circumferential radial array of bent sinusoidal flow channels.
In order to reasonably utilize space and effectively shorten the breadth of the chip, the inertia dense flow channel is composed of a plurality of bent fan-shaped channels.
In order to separate and lead out the concentrated solution and the blank solution, the branched flow passage is a non-open cross branched flow passage, the two transverse ends of the branched flow passage are respectively communicated with the inner outlet, and the vertical flow passage is communicated with the outer outlet.
In order to connect the enrichment module and the inner outlet collection module, the enrichment module and the inner outlet collection module are connected by a bonding module.
In order to lead the concentrated solution and the blank solution out of the chip for collection, the device also comprises an outlet module which is connected below the flow control module.
In order to conveniently collect and guide out the blank solution at the outlet in the enrichment module, the inner outlet collection module collects the blank solution at the inner outlet to the middle through the T-shaped flow channel. The T-shaped runners of the inner outlet collection modules are distributed in the circumferential radial direction, and the number of the T-shaped runners is consistent with that of the inertial concentration runners in the distribution mode.
In order to conveniently collect and guide out the concentrated solution at the outer outlet of the enrichment module, the outer outlet collection module collects the concentrated solution at the outer outlet to the middle through the arranged outer collecting pipeline. The external collecting runners of the external outlet collecting module are arranged in the circumferential radial direction, and the number of the external collecting runners is consistent with that of the inertial concentrating runners in the arrangement mode.
The working principle is as follows: the inertial micro-fluidic technology utilizes the combined action of inertial lift force of the inertial migration effect of particles on a micro scale and Dean drag force of secondary flow generated in a bent flow channel to accurately control the enrichment of the particles. Wherein two vortexes with opposite rotation directions are generated in a vertical main flow direction when the fluid passes through the bent flow channel, which is called Dean flow, and Dean drag force is generated due to the introduction of the Dean flow. The sample liquid is subjected to the coupling effect of inertial lift force and Dean drag force in the monomer bending type asymmetric sinusoidal flow channel, the sample liquid is introduced at a specific flow rate only by ensuring the inlet, and particles can be arranged in a row in the outlet area without any external field effect and are positioned in the center of the flow channel. By using the flow dividing function of the cross-shaped branched flow channel, the focused particles are guided out and collected by the outer outlet, and the blank sample is guided out from the two inner outlets.
Has the advantages that: the invention has high concentration efficiency, can meet the enrichment requirement of sample liquid with extremely low concentration or large volume, has wide application range, is not only suitable for scientific research in laboratories, but also can be applied to biomedical diagnosis and environmental instant detection, and has simple chip manufacture, low cost and simple use and operation.
Drawings
FIG. 1 is an exploded view of a functional module of the present invention;
FIG. 2 is a top view of an enrichment module;
FIG. 3 is a top view of a glue module;
FIG. 4 is a top view of the inner outlet collection module;
FIG. 5 is a top view of the drainage module;
FIG. 6 is a top view of the outer outlet collection module;
FIG. 7 is a top view of a flow control module;
FIG. 8 is a top view of the outlet module;
FIG. 9 is a top view of the present invention;
FIG. 10 is a graph showing the concentration variation of microalgae cell inlet and inner and outer outlets at different flow rates;
FIG. 11 is a bar graph of the change in the efficiency of microalgae cell enrichment at different flow rates.
Detailed Description
The invention will be further explained with reference to the drawings.
As shown in fig. 1, a micro-particle high-flux enrichment microfluidic chip is sequentially stacked from top to bottom and provided with an enrichment module 1, a bonding module 2, an inner outlet collection module 3, a drainage module 4, an outer outlet collection module 5, a flow control module 6 and an outlet module 7. As shown in fig. 2, the enrichment module 1 is provided with a sample inlet 11, an outer outlet 12, an inner outlet 13, an inertial concentration flow channel 14, a direct flow channel 15, and a cross-shaped branched flow channel 16; one end of an inertia concentration flow passage 14 is communicated with the sample inlet 11 through a section of straight flow passage 15, and the other end is respectively communicated with the inner outlet 13 and the outer outlet 12 through a cross-shaped branched flow passage 16; the inertial concentration flow channel 14 is a bent asymmetric sinusoidal flow channel, and the processing flux per unit time is increased by a circumferential radial array. In order to reasonably utilize the space and effectively shorten the breadth of the chip, the inertial concentration flow passage 14 is in a bent fan-shaped distribution. The inertial concentration flow passage 14, the direct flow passage 15 and the cross-shaped branched flow passage 16 are in a non-open passage form, and the cross section of the inertial concentration flow passage is of a rectangular structure. The sample inlet 11 is open upward, and the outer outlet 12 and the inner outlet 13 are open downward. As shown in fig. 3, the bonding module 2 is provided with an inner inlet 21 and an outer inlet 22; the inner inlet 21 and the outer inlet 22 are not communicated with each other and are in the form of through holes penetrating through layers. The bonding module 2 bonds the enrichment module 1 with the inner outlet collection module 3; as shown in fig. 4, the inner outlet collection module 3 is provided with an inner inlet 31, an outer inlet 32, an inner collection duct 33, and an inner collection outlet 34; one end of the inner collecting channel 33 is connected with the inner collecting outlet 34, and the other end is communicated with the two inner inlets 31 through a T-shaped structural channel 35; the inner collecting flow passages 33 are circumferentially and radially arranged, and the number and the arrangement mode are the same as those of the inertia concentration flow passages 14 on the high-flux inertia concentration module 1; the outer inlet 32 is not communicated with the inner inlet 31, the inner flow guide channel 33, the T-shaped structural flow channel 35 and the inner gathering outlet 34; the inner inlet 31 is upwardly open, the inner manifold outlet 34 is downwardly open, and the outer inlet 32 is a through-plane via. As shown in fig. 5, the drainage module 4 is provided with an outer inlet 41, a drainage flow channel 42, an inner collection inlet 43, and an inner collection outlet 44, and the drainage module 4 bonds the inner outlet collection module 3 with the outer outlet collection module 5; the outer inlet 41, the drainage flow channel 42, the inner collection inlet 43 and the inner collection outlet 44 are all of a through-layer through hole structure. As shown in fig. 6, the outer outlet collection module 5 is provided with an outer inlet 51, an outer collection flow channel 52, an inner collection outlet 53, and an outer collection outlet 54; the outer inlet 51, the outer collecting flow passage 52 and the outer collecting outlet 54 are communicated in sequence; the inner collection outlet 53 is not in communication with the outer inlet 51, the outer collection flow passage 52, and the outer collection outlet 54. The outer collecting channels 52 are circumferentially and radially arranged, and the number and the arrangement mode are the same as those of the inertia concentration channels 14 on the high-flux inertia concentration module 1; the outer inlet 51 is upwardly open, the inner collection outlet 53 is a through-layer via, the outer collection outlet 54 is downwardly open, and the outer collection flow channel 52 is in the form of a non-open channel with a rectangular cross-section. As shown in fig. 7, the flow control module 6 is provided with an internal flow guiding channel 61, an internal collection inlet 63, an internal collection outlet 64, an external flow guiding channel 62, an external collection inlet 65, and an internal collection outlet 66, the flow control module 6 simultaneously bonds the external outlet collection module 5 and the outlet module 7, and the internal flow guiding channel 61, the internal collection inlet 63, the internal collection outlet 64, the external flow guiding channel 62, the external collection inlet 65, and the internal collection outlet 66 are all of a through-layer structure. As shown in fig. 8, the outlet module 7 is provided with an inner outlet 71 and an outer outlet 72; the inner outlet 71 and the outer outlet 72 are both of a through-layer through hole structure.
When the chip is prepared, the outer outlet 12 and the inner outlet 13 on the high-flux inertial enrichment module 1 are respectively aligned with the outer outlet 22 and the inner outlet 21 on the bonding module 2, and meanwhile, the bonding module 2 plays a role in bonding the high-flux inertial enrichment module 1 and the inner outlet collection module 3. The inner outlet 21 and the outer outlet 22 on the bonding module 2 are respectively aligned with the inner inlet 31 and the outer inlet 32 of the inner outlet collection module 3; the outer inlet 32 of the inner outlet collection module 3 is aligned with the outer inlet 41 of the drainage module 4; the inner collection outlet 34 of the inner outlet collection module 3 is aligned with the drainage flow channel 42 of the drainage module 4 near the central end inlet 43, the drainage module 4 being capable of bonding the inner outlet collection module 3 and the outer outlet collection module 5; the outer inlet 41 of the drainage module 4 is aligned with the outer inlet 51 of the outer outlet collection module 5, respectively, and the non-central end outlet 44 of the drainage flow channel 42 of the drainage module 4 is aligned with the inner inlet 53 of the outer outlet collection module 5; the inner inlet 53 of the outer outlet collection module 5 is aligned with the inlet 63 of the inner flow control channel 61 of the flow control module 6; the outer collection outlet 54 of the outer outlet collection module 5 is aligned with the inlet 65 of the outer flow control channel 62 of the flow control module 6, the flow control module 6 serves for bonding the outer outlet collection module 5 to the outlet module 7, the outlet 64 of the flow control channel 61 in the flow control module 6 is aligned with the inner outlet 71 of the outlet module 7, and the outlet 66 of the outer flow control channel 62 of the flow control module 6 is aligned with the outer outlet 72 of the outlet module 7.
When the chip is used, a microparticle sample liquid flows in from the sample inlet 11, flows into each inertial concentration flow channel 14 through the direct flow channel 15, microparticles are focused in each inertial concentration flow channel 14 to be guided out of the high-flux inertial enrichment module 1 through the outer outlet 12 after being split by the cross-shaped branched flow channel 16, and a blank solution without microparticles is guided out of the inner outlet 13, as shown in fig. 9. And removing the blank solution, namely, improving the concentration of the sample solution in one-time use. The blank solution led out from the inner outlet 13 of the high-flux inertial enrichment module 1 flows through the upper inner inlet 21 of the bonding module 2, flows downwards into the upper inner inlet 31 of the inner collection module 3, the inner convergence flow channel 33 and the inner convergence outlet 34, flows downwards after being converged into the upper drainage flow channel 42 of the drainage module 4 close to the central end inlet 43, flows downwards into the upper inner convergence outlet 53 of the outer outlet collection module 5 from the outlet 44 of the drainage flow channel 42, flows downwards into the inlet 63 of the inner flow control channel 61 of the flow control module 6, and flows downwards into the upper inner outlet 71 of the outlet module 7 from the outlet 64 of the inner flow control channel 61 to be led out of the whole chip for collection. Similarly, the concentrated corpuscle liquid flow led out from the outer outlet 12 of the high flux inertia concentration module 1 flows through the upper outer inlet 22 of the bonding module 2, flows downwards into the upper outer inlet 32 of the inner collection module 3, flows downwards into the upper outer inlet 41 of the drainage module 4, flows downwards into the upper outer inlet 51 of the outer outlet collection module 5, is collected by the outer collection flow channel 52, then flows downwards into the inlet 65 of the outer flow control channel 62 of the flow control module 6 through the outer collection outlet 54, and is led out of the whole chip collection through the outlet 66 to the upper outer outlet 72 of the lower flow control module 7.
The first layer enrichment module 1 is a core layer and comprises monomer bending type asymmetric sinusoidal channels which are radially distributed, cell sample liquid is led in from a central inlet, the monomer bending type asymmetric sinusoidal channels are subjected to the coupling action of inertial lift force and Dean drag force, the inlet is led in at a specific flow rate, particles can be arranged in a row in an outlet region without any external field action, and the particles are located in the center of the channels. By using the flow splitting effect of the cross-shaped branched flow channel, the focused particle beams are guided out and collected by the outer outlet, and the blank sample is guided out from the two inner outlets. The concentration of the sample liquid collected by the outer outlet is obviously improved due to the derivation of the blank sample liquid without particles. The third layer inner outlet collection module 3 is a collection layer of inner outlet blank samples and is used for collecting and collecting the blank sample liquid collected by the inner outlet to the middle. The fourth layer of drainage module 4 is used for bonding and drainage, and is used for draining the blank sample layer collected in the third layer to the side edge, and the middle area is left out, so that an outlet structure can be arranged in the subsequent process. The fifth layer of the external outlet collection module 5 is used for collecting and draining the enriched and concentrated sample liquid led out from the external outlet to the middle. The sixth layer of flow control module 6 is mainly used for matching the flow resistance of the outlet, so that the volume of the sample flow finally led out from the seventh layer of outlet module 7 is fixed, the concentration efficiency is not influenced by the complicated pipelines of 2-6 layers, and the two flow resistances are adjusted by controlling the width of the upper drainage channel.
The process of enriching the Lankania macrorrhiza cells by using the chip of the invention comprises the following steps: firstly, manufacturing a chip, wherein the enrichment module 1, the inner outlet collection module 3, the outer outlet collection module 5 and the outlet module 7 are made of PVC plastics; the bonding module 2, the drainage module 4 and the flow rate control proportion module 6 are made of double-sided adhesive tape. The enrichment module 1, the bonding module 2, the inner outlet collection module 3, the drainage module 4, the outer outlet collection module 5, the flow control proportion module 6 and the outlet module 7 all adopt laser processing to cut out a required structure. Required runner structures are cut out on the selected PVC substrate and the plastic packaging film by using a laser respectively during manufacturing of the enrichment module 1, the inner outlet collection module 3 and the outer outlet collection module 5, and packaging is completed by using a plastic packaging machine, so that the processing time of the technology is short, the processing time is less than 1 min/piece, the processing precision is high, the deviation is about 5 microns, the manufacturing cost is low, and the flexibility is extremely strong. Then placing the chip in a clamp; taking the alga species of the Alstonia platensis to be enriched, sucking 30ml of sample liquid of the Alstonia platensis into an injector, connecting the head of the injector with the inlet of a clamp, connecting outlets at two sides with two containers, placing the injector above an injection pump, and setting a proper flow rate until all liquid in the injector is pushed; collecting the solution in two containers to obtain concentrated solution. Wherein, the operation flow rate is set to be 1-8ml/min, wherein, one flow rate is selected each time, the change curve of the cell concentration in the concentrated solution collected by the initial sample of the sample inlet 11, the inner outlet 71 and the outer outlet 72 is measured and obtained as shown in fig. 10, the concentration efficiency is calculated by the following calculation formula:
the calculated concentration efficiency results are shown in fig. 11, and show that the integrated chip has the best concentration effect when the sample driving flow is 6 ml/min, and the cell concentration at the sample inlet 11 is 1.98 x 105Cell/ml, concentration of 0.351 x 10 cells in concentrate collected at internal outlet 715Cell/ml, and the concentration of cells in the concentrate collected at the outer outlet 72 was 4.52 x 105The concentration times of the concentrated solution are 2.28 times.
Claims (10)
1. The micro-fluidic chip for high-flux enrichment of micro-particles is characterized in that an enrichment module (1), an inner outlet collection module (3), a drainage module (4), an outer outlet collection module (5) and a flow control module (6) are sequentially stacked from top to bottom, a sample inlet (11) is formed in the center of the enrichment module (1), the sample inlet (11) is communicated with an inertial concentration flow channel (14), the inertial concentration flow channel (14) is communicated with a branched flow channel (16), the branched flow channel (16) is communicated with an inner outlet (13) and an outer outlet (12), the inner outlet collection module (3) collects a blank solution of an inner outlet (13) of the enrichment module in the middle, the drainage module (4) drains the blank solution collected by the inner outlet collection module (3) to the side, the outer outlet collection module (5) collects a concentrated solution of the outer outlet (12) in the middle, and the flow control module (6) limits the flow resistance of the blank solution and the concentrated solution and then leads out the blank solution and the concentrated solution.
2. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 1, wherein the inertial concentration flow channel (14) is a circumferential radial array of bent sinusoidal flow channels.
3. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 1, wherein the inertial dense flow channel (14) is composed of a plurality of bent sectors.
4. The micro-fluidic chip for high-throughput enrichment of micro-particles according to claim 1, wherein the branched flow channel (16) is a non-open cross branched flow channel, the two transverse ends of the branched flow channel are respectively communicated with the inner outlet (13), and the vertical flow channel is communicated with the outer outlet (12).
5. The microparticle high-throughput enrichment microfluidic chip according to claim 1, wherein the enrichment module (1) and the inner outlet collection module (3) are connected through a bonding module (2).
6. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 1, further comprising an outlet module (7) connected below the flow control module (6).
7. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 1, wherein the inner outlet collection module (3) collects the blank solution of the inner outlet (13) to the middle through the arranged T-shaped flow channel (35).
8. The micro fluidic chip for high throughput enrichment of micro particles according to claim 7, wherein the T-shaped channels (35) of the inner outlet collection module (3) are circumferentially and radially arranged in the same number as the inertial concentration channels (14).
9. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 1, wherein the outer outlet collection module (5) collects the concentrated solution of the outer outlet (12) to the middle through the arranged outer collection channel (52).
10. The micro-fluidic chip for high throughput enrichment of micro-particles according to claim 9, wherein the outer collection channels (52) of the outer outlet collection module (5) are circumferentially and radially arranged in the same number as the inertial concentration channels (14).
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| CN111330660B (en) * | 2020-03-10 | 2022-01-25 | 中国科学院苏州生物医学工程技术研究所 | Centrifugal high-flux micro-droplet preparation chip |
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| AU2016389767B2 (en) * | 2016-01-28 | 2019-08-01 | Biolidics Limited | Multi-stage target cell enrichment using a microfluidic device |
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| CN102513169A (en) * | 2011-12-09 | 2012-06-27 | 东南大学 | Microfluidic device used in micron-grade particle high-flux separation, and manufacturing method thereof |
| US9789485B2 (en) * | 2012-09-21 | 2017-10-17 | Massachusetts Institute Of Technology | Micro-fluidic device and uses thereof |
| CN203220910U (en) * | 2013-03-01 | 2013-10-02 | 东南大学 | Integrated chip for high-throughput sorting and count detection of biological particles |
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