CN108160126A - Micro particles high throughput is enriched with micro-fluidic chip - Google Patents
Micro particles high throughput is enriched with micro-fluidic chip Download PDFInfo
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- CN108160126A CN108160126A CN201711248886.1A CN201711248886A CN108160126A CN 108160126 A CN108160126 A CN 108160126A CN 201711248886 A CN201711248886 A CN 201711248886A CN 108160126 A CN108160126 A CN 108160126A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
Abstract
The present invention discloses a kind of micro particles high throughput enrichment micro-fluidic chip, it stacks gradually from top to down and is provided with enrichment module, inner outlet collection module, drain module, outer vent collection module and flow control module, the enrichment module is provided centrally with sample inlet, the sample inlet is communicated with inertia concentration runner, the inertia concentration runner is communicated with bifurcated runner, the bifurcated runner connection inner outlet and outer vent, the blank solution of the enrichment module inner outlet is pooled to centre by the inner outlet collection module, the blank solution that the inner outlet collection module collects is drained to side by the drainage module, the concentrate solution of the outer vent is pooled to centre by the outer vent collection module, the flow control module exports after being defined to the flow resistance of blank solution and concentrate solution.Thickening efficiency of the present invention is high, can meet the enrichment demand of extremely low concentration or bulk sample liquid, have a wide range of application, chip manufacturing is simple, of low cost, and use is easy to operate.
Description
Technical field
The present invention relates to a kind of enrichment chips, and in particular to a kind of micro particles high throughput is enriched with micro-fluidic chip.
Background technology
Nowadays particulate beneficiation technologies are nearly ubiquitous, it has also become biological study, chemical analysis, environment measuring and medical treatment
Important sample pretreatment link in numerous industries such as diagnosis.
And particle enrichment main method is centrifugal process and fracture filtration method at present.Centrifugal process is mainly made molten by high speed rotation
Particle in liquid settles, then supernatant is gone to obtain pregnant solution.This method generally requires, by expensive instrument, to lead to this
Method is had no idea for field or remote poverty-stricken area, while high speed centrifugation makes the flexible biological sample extremely dilute to concentration
Into inevitable injury.Compared to high speed centrifugation method, the principle of fracture filtration then seems more simple, this method major part
It is the film containing specific dimensions microcellular structure, which, which is placed in filtering mouth, can obstruct solid constituent in the sample, must be big
In micropore size, filter walks blank solution to achieve the purpose that enrichment.Since microporous barrier easily blocks, accumulate a certain amount of solid into
/ after, sample liquid is difficult to smoothly flow out, therefore can not meet the enrichment demand of high concentration or large volume sample liquid.This
Outside, for how to remove the problem of being sticked to particle on film, current means are still difficult to solve.Both methods the former mainly use
In laboratory and hospital, the latter is mostly used for obtaining clear liquid at present.
Invention content
Goal of the invention：The present invention provides a kind of micro particles high throughput enrichment micro-fluidic chip, solves existing particle enrichment
Method thickening efficiency is low, it is impossible to meet the enrichment demand of extremely low concentration or bulk sample liquid, application range is narrow, inconvenient.
Technical solution：Micro particles high throughput of the present invention is enriched with micro-fluidic chip, stacks gradually set from top to down
It is equipped with enrichment module, inner outlet collection module, drains module, outer vent collection module and flow control module, in the enrichment module
The heart is provided with sample inlet, and the sample inlet is communicated with inertia concentration runner, and the inertia concentration runner is communicated with bifurcated stream
Road, bifurcated runner connection inner outlet and outer vent, the inner outlet collection module is by the sky of the enrichment module inner outlet
White solution is pooled to centre, and the blank solution that the inner outlet collection module collects is drained to side by the drainage module, institute
It states outer vent collection module and the concentrate solution of the outer vent is pooled to centre, the flow control module is to blank solution and concentration
The flow resistance of solution exports after being defined.
In order to improve the unit interval processing sample liquid flux, inertia concentration runner is the curved of circumference radial arrays
Folding sine runner.
In order to rationally utilize space, effectively shorten the breadth of chip, the sector that the dense runner of inertia is bent by several
Composition.
In order to which concentrate solution and blank solution separation are exported, the bifurcated runner is non-unlimited cross bifurcated runner, horizontal
Inner outlet, vertical-sprue connection outer vent are respectively communicated with to both ends.
In order to which enrichment module is connected with inner outlet collection module, lead between the enrichment module and inner outlet collection module
It crosses and bonds module connection.
In order to which concentrate solution and blank solution export chip are collected, outlet module is further included, is connected to flow control
Below module.
The blank solution of enrichment module inner outlet is collected into export for convenience, the inner outlet collection module passes through setting
T-shaped runner inner outlet blank solution is pooled to centre.The T-shaped runner of the inner outlet collection module is radially arranged in week in week
Cloth, quantity are consistent with inertia concentration runner with arrangement mode.
The concentrate solution of enrichment module outer vent is collected into export for convenience, the outer vent collection module passes through setting
Foreign exchange mass flow pathway outer vent concentrate solution is pooled to centre.The foreign exchange mass flow pathway of the outer vent collection module is in week Zhou Jing
To arrangement, quantity is consistent with inertia concentration runner with arrangement mode.
Operation principle：The inertia that inertia microflow control technique of the present invention is subject to using the inertia migration effect under particle minute yardstick
Lift and the Secondary Flow that is generated in curved runner and particle enrichment is accurately manipulated by the collective effect of Dean drag.Its
In, the opposite vortex in two direction of rotation is generated in vertical main flow direction when fluid passes through curved runner, is referred to as Dean
Stream generates Dean drag due to the introducing of Dean streams.Sample liquid is in the asymmetric sinusoidal runner of monomer folding type by inertia liter
The coupling of power and Dean drag only need to ensure that entrance is imported with specific flow velocity, may be such that without any outer field action micro-
Grain is arranged in a row in exit region, and positioned at runner center.The shunting function of cross bifurcated runner, the row of focusing are utilized again
The particle of row will be exported by outer vent and be collected, and blank sample is exported from two inner outlets.
Advantageous effect：Thickening efficiency of the present invention is high, can meet the enrichment demand of extremely low concentration or bulk sample liquid, application
Range is wide, is applicable not only to laboratory science research, is equally applicable for biomedical diagnostic, environment detects immediately, chip manufacturing
Simply, of low cost, use is easy to operate.
Description of the drawings
Fig. 1 is function module explosive view of the present invention；
Fig. 2 is the vertical view of enrichment module；
Fig. 3 is the vertical view for being bonded module；
Fig. 4 is the vertical view of inner outlet collection module；
Fig. 5 is the vertical view for draining module；
Fig. 6 is the vertical view of outer vent collection module；
Fig. 7 is the vertical view of flow control module；
Fig. 8 is the vertical view of outlet module；
Fig. 9 is the vertical view of the present invention；
Figure 10 is the concentration curve of lower microalgae cell entrance different in flow rate and interior outer vent；
Figure 11 is the variation block diagram of lower microalgae cell bioaccumulation efficiency different in flow rate.
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings.
As shown in Figure 1, a kind of micro particles high throughput enrichment micro-fluidic chip, stacks gradually be provided with enrichment from top to down
Module 1, bonding module 2, inner outlet collection module 3, drainage module 4, outer vent collection module 5, flow control module 6 and outlet module
7.As shown in Fig. 2, sample inlet 11, outer vent 12, inner outlet 13, inertia concentration runner 14, direct current are provided in enrichment module 1
Road 15, cross bifurcated runner 16；Inertia concentrates 14 one end of runner and connects sample inlet 11 by one section of straight channel 15, and the other end leads to
It crosses cross bifurcated runner 16 and is respectively communicated with inner outlet 13 and outer vent 12；Inertia concentration runner 14 is the asymmetric sinusoidal stream of folding type
Road, and improve by circumference radial arrays the processing flux of unit interval.Rationally to utilize space, effectively shorten the width of chip
The fan-shaped distribution of bending is presented in face, inertia concentration runner 14.Inertia concentration runner 14, straight channel 15, cross bifurcated runner 16 are non-
Open channels form, rectangular cross-section structure.11 upward trepanning of sample inlet, the 13 downward trepanning of outer vent 12 and inner outlet.Such as
Shown in Fig. 3, it is bonded in module 2 and is provided with interior entrance 21, outer entrance 22；Interior entrance 21, outer entrance 22 do not connect mutually, respectively wear
Layer through-hole form.It is bonded module 2 enrichment module 1 is be bonded with inner outlet collection module 3；As shown in figure 4, inner outlet collection module
Interior entrance 31 is provided on 3, outer entrance 32, interior collect runner 33, interior collect outlet 34；Inside collect and converged in the connection of 33 one end of runner
Collection outlet 34, the other end connects two interior entrances 31 by T-shaped structure runner 35；Inside collect runner 33 circumferentially radially to arrange,
Quantity and arrangement mode are identical with the inertia concentration runner 14 in high-throughput inertia enrichment module 1；Outer entrance 32 and interior entrance 31,
Interior flow-guiding channel 33, T-shaped structure runner 35, the interior outlet 34 that collects are not communicated with；Interior entrance 31 is upward trepanning, inside collects outlet
34 be downward trepanning, and outer entrance 32 is wears a layer through-hole.As shown in figure 5, the outer entrance 41 of setting in drainage module 4, drain flow path 42,
Inside collect entrance 43, it is interior collect outlet 44, the drainage module 4 glues inner outlet collection module 3 and outer vent collection module 5
It connects；The outer entrance 41, drain flow path 42, it is interior collect entrance 43, the interior outlet that collects 44 is to wear a layer through-hole structure.Such as Fig. 6 institutes
Show, be provided on outer vent collection module 5 outer entrance 51, foreign exchange mass flow pathway 52, it is interior collect outlet 53, exchange set outlet 54；Outside
Entrance 51, foreign exchange mass flow pathway 52, exchange set outlet 54 are sequentially communicated；Inside collect outlet 53 with outer entrance 51, foreign exchange mass flow pathway 52,
Exchange set exports 54 not intercommunications.The circumferentially radially arrangement of foreign exchange mass flow pathway 52, quantity and arrangement mode and high-throughput inertia are rich
The inertia concentration runner 14 collected in module 1 is identical；Outer 51 upward trepanning of entrance inside collects outlet 53 to wear a layer through-hole, exchange set
Export 54 downward trepannings, foreign exchange mass flow pathway 52, for non-open channels form, rectangular cross-section structure.As shown in fig. 7, flow control mould
Be provided on block 6 interior drain flow path 61, it is interior collect entrance 63, it is interior collect outlet 64, external drainage runner 62, exchange set entrance 65,
Inside collect outlet 66, outer vent collection module 5 is be bonded with outlet module 7, interior drain flow path 61, the interior remittance simultaneously of flow control module 6
Collection entrance 63, it is interior collect outlet 64, external drainage runner 62, exchange set entrance 65, the interior outlet that collects 66 are to wear a layer structure.Such as figure
Shown in 8, inner outlet 71, outer vent 72 are provided on outlet module 7；Inner outlet 71, outer vent 72 are to wear a layer through-hole structure.
When preparing said chip, outer vent 12, inner outlet 13 in high-throughput inertia enrichment module 1 respectively with Nian Jie module
Outer vent 22, inner outlet 21 on 2 are aligned, while are bonded module 2 and are played the high-throughput inertia enrichment module 1 of bonding and inner outlet receipts
Collect the purpose of module 3.Be bonded module 2 on inner outlet 21, outer vent 22 respectively with the interior entrance 31 of inner outlet collection module 3,
Outer entrance 32 is aligned；The outer entrance 32 of inner outlet collection module 3 is aligned with draining the outer entrance 41 of module 4；Inner outlet collects mould
Collect outlet 34 in block 3 and be aligned with draining the drain flow path 42 of module 4 close to center end entrance 43, drainage module 4 can glue
Connect inner outlet collection module 3 and outer vent collection module 5；Drain outer entrance 41 and the outer vent collection module 5 of module 4 it is outer enter
Mouthfuls 51 are respectively aligned to, and the drain flow path 42 for draining module 4 non-central brings out mouth 44 and the interior entrance 53 of outer vent collection module 5 is right
It is accurate；The interior entrance 53 of outer vent collection module 5 is aligned with 61 entrance 63 of interior flow control channel of flow control module 6；Outer vent is received
The exchange set outlet 54 of collection module 5 is aligned with 62 entrance 65 of external flux control channel of flow control module 6, and flow control module 6 plays outer
The bonding effect of collection module 5 and outlet module 7 is exported, flow control channel 61 exports 64 and outlet module 7 in flow control module 6
Inner outlet 71 be aligned, the outlet of 6 external flux control channel 62 of flow control module 66 is aligned with the outer vent 72 of outlet module 7.
Vertical view after each layer vertical stacking is as shown in figure 9, during using the chip, and particulate sample liquid is by sample inlet
11 flow into, and flowing into each branch inertia by straight channel 15 concentrates runner 14, and particulate will be gathered in each branch inertia concentrates runner 14
Coke is to width of flow path intermediate region, so as to export high-throughput inertia enrichment mode by outer vent 12 after the shunting of cross bifurcated runner 16
Block 1, and the blank solution without particulate is exported by inner outlet 13.Blank solution removes, you can sample is improved in first use
The concentration of product liquid.The blank solution as derived from high-throughput 1 inner outlet 13 of inertia enrichment module flows through interior entrance in bonding module 2
21, flow downwardly into interior entrance 31 on interior collection module 3, it is interior collect runner 33, it is interior collect outlet 34, drainage is flowed downwardly into after collecting
Drain flow path 42 is close to center end entrance 43 in module 4, and exports 44 by drain flow path 42 and flow downwardly into outer vent collection module 5
Collect outlet 53 in upper, flow downwardly into 61 entrance 63 of flow control channel in flow control module 6, exported by interior flow control channel 61
64, which flow downwardly into inner outlet 71 on outlet module 7, exports entire chip collection.Equally, it is gone out by high-throughput inertia enrichment module 1
Particulate liquid stream flows downwardly into outer entrance on interior collection module 3 through being bonded outer entrance 22 in module 2 after the derived concentration of mouth 12
32, outer entrance 41 in drainage module 4 is flowed downwardly into, outer entrance 51 on outer vent collection module 5 is flowed downwardly into, through foreign exchange mass flow pathway
52 collect after again exchange set outlet 54 collects flow into 62 entrance 65 of flow control module 6 external flux control channel downwards, from outlet 66 to
Outer vent 72 exports entire chip and collects in the inlet module 7 that flows down.
Wherein, first layer enrichment module 1 is core layer, wherein including the asymmetric sinusoidal stream of radially-arranged monomer folding type
Road, cell sample liquid are imported by central inlet, in the asymmetric sinusoidal runner of monomer folding type by inertia lift and Dean drag
Coupling, only need to ensure that entrance is imported with specific flow velocity, without any outer field action can by particle exit region row
Arrange it is in a row, and positioned at runner center.Using the shunting function of cross bifurcated runner, the beam of corpuscular for focusing on arrangement will be by
Outer vent export is collected, and blank sample is exported from two inner outlets.Since the export for being free of particle blank sample liquid will cause
The sample liquid concentration that outer vent is collected is significantly improved.Third layer inner outlet collection module 3 is the remittance of inner outlet blank sample
Collect layer, the blank sample liquid collection for inner outlet to be collected converges to centre.4th layer of drainage module 4 is that bonding plus drainage are made
With, for the blank sample collected in third layer layer to be drained to side, vacate intermediate region, so as to subsequently arrange outlet tie
Structure.Layer 5 outer vent collection module 5 is the sample liquid after the derived enrichment concentration of outer vent, collects and is drained to centre.6th
Layer flow control module 6 is mainly used for the flow resistance of matching outlet, so finally from the body of sample flow derived from layer 7 outlet module 7
Product be it is fixed, thickening efficiency will not because of we 2-6 layers complex pipeline and be affected, this layer by control above draw
The width of circulation road adjusts the flow resistance of two.
It is to be enriched with Platymonas helgolandica var cell processes using chip of the invention：One piece of said chip, enrichment are made first
The material selection PVC plastic of module 1, inner outlet collection module 3, outer vent collection module 5 and outlet module 7；Module 2 is bonded,
Module 4 is drained, the material of flow control amount ratio module 6 is double faced adhesive tape.Enrichment module 1, bonding module 2, inner outlet collection module 3,
Drainage module 4, outer vent collection module 5, flow control amount ratio module 6 and outlet module 7 are using Laser Processing to be cut into
The structure needed.In the PVC substrates and plastic packaging of selection when enrichment module 1, inner outlet collection module 3, outer vent collection module 5 make
Required flow passage structure is cut into laser respectively on film, plastic packaging machine is reused and completes encapsulation, this technology process time is short, processing
Time<1min/ pieces, high precision machining, about 5 μm of deviation, low manufacture cost, flexibility are extremely strong.Then by chip be placed in fixture it
In；Platymonas helgolandica var algae to be enriched with is taken, Platymonas helgolandica var algae sample liquid 30ml is drawn into syringe, and by syringe
Head is connect with fixture entrance, and two side outlets are connected with two containers, and it is supreme that syringe is placed in syringe pump, and it is suitable to set
Flow velocity, until liquid has all pushed away in syringe；Concentrate can be obtained by collecting the solution of two containers.Wherein, setting operation
Flow velocity is in 1-8ml/min, wherein choosing a flow velocity every time, measures the initial sample for obtaining sample inlet 11, inner outlet 71 also
The change curve for having cell concentration in the concentrate of the collection of outer vent 72 is as shown in Figure 10, calculates thickening efficiency, calculation formula is such as
Under：
The thickening efficiency result of calculating is as shown in figure 11, the results showed that, it, should when sample driving flow is 6 ml/min
Integrated chip has best concentrated effect, and 11 cell concentration of sample inlet is 1.98*105A/ml, inner outlet 71 are collected dense
Cell concentration is 0.351*10 in contracting liquid5A/ml, and cell concentration is 4.52*10 in the concentrate that outer vent 72 is collected5A/
Ml, cycles of concentration reach 2.28 times.
Claims (10)
1. a kind of micro particles high throughput is enriched with micro-fluidic chip, which is characterized in that stacks gradually be provided with enrichment from top to down
Module (1), inner outlet collection module (3), drainage module (4), outer vent collection module (5) and flow control module (6), the enrichment
Module (1) is provided centrally with sample inlet (11), and the sample inlet (11) is communicated with inertia concentration runner (14), the inertia
Concentration runner (14) is communicated with bifurcated runner (16), bifurcated runner (16) the connection inner outlet (13) and outer vent (12), institute
It states inner outlet collection module (3) and the blank solution of the enrichment module inner outlet (13) is pooled to centre, the drainage module
(4) blank solution that the inner outlet collection module (3) is collected is drained to side, the outer vent collection module (5) is by institute
The concentrate solution for stating outer vent (12) is pooled to centre, the flow control module (6) to the flow resistance of blank solution and concentrate solution into
Row exports after limiting.
2. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the inertia concentration
Runner (14) is the folding type sine runner of circumference radial arrays.
3. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the dense stream of inertia
Road (14) is made of several sectors bent.
4. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the bifurcated runner
(16) it is non-unlimited cross bifurcated runner, transverse ends are respectively communicated with inner outlet (13), vertical-sprue connection outer vent (12).
5. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the enrichment module
(1) it is connected between inner outlet collection module (3) by bonding module (2).
6. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that further includes outlet mold
Block (7) is connected to below flow control module (6).
7. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the inner outlet is received
Inner outlet (13) blank solution is pooled to centre by collection module (3) by the T-shaped runner (35) of setting.
8. micro particles high throughput according to claim 7 is enriched with micro-fluidic chip, which is characterized in that the inner outlet is received
The T-shaped runner (35) for collecting module (3) is radially arranged in all, and quantity concentrates runner (14) one with arrangement mode and the inertia
It causes.
9. micro particles high throughput according to claim 1 is enriched with micro-fluidic chip, which is characterized in that the outer vent is received
Outer vent (12) concentrate solution is pooled to centre by collection module (5) by the foreign exchange mass flow pathway (52) of setting.
10. the micro particles high throughput enrichment micro-fluidic chip according to right wants 9, which is characterized in that the outer vent is received
The foreign exchange mass flow pathway (52) for collecting module (5) is radially arranged in all, and quantity is with arrangement mode and inertia concentration runner (14)
Unanimously.
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| CN111330660A (en) * | 2020-03-10 | 2020-06-26 | 中国科学院苏州生物医学工程技术研究所 | Centrifugal high-flux micro-droplet preparation chip |
| CN112903411A (en) * | 2021-01-25 | 2021-06-04 | 东南大学 | Multi-mode biological particle concentrator |
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