Invention content
The technical problems to be solved by the invention are, provide a kind of growth period duration of rice pest control method, utilize widow
Male rotten mould aggressiveness, parasitics characteristic effectively prevent the diseases such as bakanae disease of rice, damping-off, rice blast.
In order to solve the above technical problems, the present invention provides a kind of growth period duration of rice pest control method, including following step
Suddenly:
A. the selection of test site;
B. selection, cleaning, the presoaking and germinating early-stage preparations of seed rice;
C. paddy seedbed sowing, rice transplant crop field from seedbed;
D. tested in the disease big generation phase, with clear water, comparison medicament, pythium oligandrum WP on Rice into
Row sprinkling dispenser;
E. in each processing seedling-cultivation plate rice shoot of rice disease occurrence in peak period investigation, there is a situation where diseases.
The step b further comprises:Selection grain is full greatly, and evengranular seed takes high-temperature sterilization.
The high-temperature sterilization further comprises:One basin temperature of hot water taking is controlled between 50-60 degrees Celsius, then will
Rice paddy seed impregnates in water, and is stirred continuously, until the near normal temperature of water temperature.
The step b further comprises:Seed impregnate 1-2 days using 25 degrees Celsius of warm water, vernalization.
The step c further comprises:Seeding in nursery bed is sowed uniformly, and light seeding amount is sowed and is not overlapped uniformly, shallow earthing,
Thickness of earth-fill cover is no more than 0.7cm, after planting wants strict temperature control between 28-30 DEG C, and thermometer is placed on counterdie,
Temperature is more than 32 DEG C aeration-cooling immediately.
The step d further comprises:Bakanae disease of rice is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
3. comparison medicament 25% Prochloraz missible oil, 3000 times of liquid are soaked seed 7 days;
4. clear water compares, soak seed 7 days.
Totally 4 processing, by seed and liquid 1.0:1.2 ratio is soaked seed, seedbed plot area 4m2, random district's groups
Arrangement, 3 repetitions.
The step d further comprises:Rice seedling blight is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, founds needle phase even spraying, by seedbed in rice
Spray is saturating, in the 2nd same dosage spray of damping-off big generation phase;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. 35% 2000 times of liquid of first frost thiram wettable powder of comparison medicament, spraying time, number and method exist together
Reason is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
Each secondary dispenser respectively handled carries out on seedbed;3 repetitions are often handled, include 5 disk rice shoots.
The step d further comprises:Rice blast is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, during entire experiment dispenser twice, respectively
Boot stage and full heading time;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. comparison medicament, 40% 1000 times of Isoprothiolane EC liquid, the same processing of spraying time, number and method is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
3 repetitions are each handled, altogether 12 cells, plot area 25m2, the random district's groups arrangement of cell.
In order to solve the above technical problems, the present invention also provides a kind of screening technique of bakanae disease of rice controlling mode, altogether
If 4 processing:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
3. comparison medicament 25% Prochloraz missible oil, 3000 times of liquid are soaked seed 7 days;
4. clear water compares, soak seed 7 days.
Press seed and liquid 1.0:1.2 ratio is soaked seed, seedbed plot area 4m2, random district's groups arrangement, 3 weights
It is multiple.
In order to solve the above technical problems, invention further provides a kind of screening technique of rice seedling blight controlling mode, altogether
If 4 processing:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, founds needle phase even spraying, by seedbed in rice
Spray is saturating, in the 2nd same dosage spray of damping-off big generation phase;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. 35% 2000 times of liquid of first frost thiram wettable powder of comparison medicament, spraying time, number and method exist together
Reason is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
Each secondary dispenser respectively handled carries out on seedbed;3 repetitions are often handled, include 5 disk rice shoots.
Advantageous effect of the present invention includes:A kind of growth period duration of rice pest control method is provided, utilizes attacking for pythium oligandrum
Hitting property, parasitics characteristic effectively prevent the diseases such as bakanae disease of rice, damping-off, rice blast.
Specific embodiment
The present invention is described in detail with reference to embodiment.To make the objectives, technical solutions, and advantages of the present invention clearer, bright
Really, the present invention is described in more detail by the following examples, but the invention is not limited in these embodiments.
Laboratory operating procedures of the present invention include:
A. the selection of test site;
B. the early-stage preparations such as selection, cleaning, presoaking and germinating of seed rice;
C. paddy seedbed sowing, rice transplant crop field from seedbed;
D. tested in the disease big generation phase, with clear water, comparison medicament, pythium oligandrum WP on Rice into
Row sprinkling dispenser;
E. in each processing seedling-cultivation plate rice shoot of rice disease occurrence in peak period investigation, there is a situation where diseases.
This sample plot with being chosen at Qingdao City Licang District white clay park Qingdao seawater rice research and development center, this area
Gesture is flat, soil property and fertility uniformity.Field is covered without weeds and algae during experiment.
Select grain full greatly, evengranular seed takes high-temperature sterilization, and one basin temperature of hot water taking is controlled in 50-60
Between degree Celsius, then rice paddy seed is impregnated in water, and is stirred continuously, until the near normal temperature of water temperature.It adopts
Seed impregnate 1-2 days with 25 degrees Celsius of warm water, vernalization.
Seeding in nursery bed is sowed uniformly, and light seeding amount is sowed and is not overlapped uniformly, shallow earthing, and thickness of earth-fill cover is no more than 0.7cm,
Strict temperature control is after planting wanted between 28-30 DEG C, thermometer is placed on counterdie, and temperature is divulged information immediately more than 32 DEG C
Cooling.
In order to solve the above technical problems, the present invention provides a kind of growth period duration of rice pest control method, including following step
Suddenly:
A. the selection of test site;
B. selection, cleaning, the presoaking and germinating early-stage preparations of seed rice;
C. paddy seedbed sowing, rice transplant crop field from seedbed;
D. tested in the disease big generation phase, with clear water, comparison medicament, pythium oligandrum WP on Rice into
Row sprinkling dispenser;
E. in each processing seedling-cultivation plate rice shoot of rice disease occurrence in peak period investigation, there is a situation where diseases.
The step b further comprises:Selection grain is full greatly, and evengranular seed takes high-temperature sterilization.
The high-temperature sterilization further comprises:One basin temperature of hot water taking is controlled between 50-60 degrees Celsius, then will
Rice paddy seed impregnates in water, and is stirred continuously, until the near normal temperature of water temperature.
The step b further comprises:Seed impregnate 1-2 days using 25 degrees Celsius of warm water, vernalization.
The step c further comprises:Seeding in nursery bed is sowed uniformly, and light seeding amount is sowed and is not overlapped uniformly, shallow earthing,
Thickness of earth-fill cover is no more than 0.7cm, after planting wants strict temperature control between 28-30 DEG C, and thermometer is placed on counterdie,
Temperature is more than 32 DEG C aeration-cooling immediately.
The step d further comprises:Bakanae disease of rice is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
3. comparison medicament 25% Prochloraz missible oil, 3000 times of liquid are soaked seed 7 days;
4. clear water compares, soak seed 7 days.
Totally 4 processing, by seed and liquid 1.0:1.2 ratio is soaked seed, seedbed plot area 4m2, random district's groups
Arrangement, 3 repetitions.
The step d further comprises:Rice seedling blight is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, founds needle phase even spraying, by seedbed in rice
Spray is saturating, in the 2nd same dosage spray of damping-off big generation phase;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. 35% 2000 times of liquid of first frost thiram wettable powder of comparison medicament, spraying time, number and method exist together
Reason is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
Each secondary dispenser respectively handled carries out on seedbed;3 repetitions are often handled, include 5 disk rice shoots.
The step d further comprises:Rice blast is tested:Experiment sets 4 processing altogether:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, during entire experiment dispenser twice, respectively
Boot stage and full heading time;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. comparison medicament, 40% 1000 times of Isoprothiolane EC liquid, the same processing of spraying time, number and method is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
3 repetitions are each handled, altogether 12 cells, plot area 25m2, the random district's groups arrangement of cell.
In order to solve the above technical problems, the present invention also provides a kind of screening technique of bakanae disease of rice controlling mode, altogether
If 4 processing:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder is soaked seed 7 days;
3. comparison medicament 25% Prochloraz missible oil, 3000 times of liquid are soaked seed 7 days;
4. clear water compares, soak seed 7 days.
Press seed and liquid 1.0:1.2 ratio is soaked seed, seedbed plot area 4m2, random district's groups arrangement, 3 weights
It is multiple.
In order to solve the above technical problems, invention further provides a kind of screening technique of rice seedling blight controlling mode, altogether
If 4 processing:
①1×106Spore/6000 times of liquid of g pythium oligandrums wettable powder, founds needle phase even spraying, by seedbed in rice
Spray is saturating, in the 2nd same dosage spray of damping-off big generation phase;
②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder, spraying time, number and method are the same as processing
①;
3. 35% 2000 times of liquid of first frost thiram wettable powder of comparison medicament, spraying time, number and method exist together
Reason is 1.;
4. clear water compares, the same processing of spraying time, number and method is 1.;
Each secondary dispenser respectively handled carries out on seedbed;3 repetitions are often handled, include 5 disk rice shoots.
(1) bakanae disease of rice is tested:Experiment sets 4 processing altogether:①1×106Spore/g pythium oligandrum wettable powders
6000 times of liquid (preparation dosage 0.5g/m 2, to water 3kg) are soaked seed 7 days. ②1×106Spore/g pythium oligandrum wettable powders
3000 times of liquid (preparation dosage 1.0g/m2, to water 3kg), it soaks seed 7 days.3. comparison medicament 25% Prochloraz missible oil, 3000 times of liquid,
Seed soaking 7 days;4. clear water compares (CK), soak seed 7 days.Totally 4 processing, by seed and liquid 1.0:1.2 ratio is soaked seed,
Seedbed plot area 4m2, random district's groups arrangement, 3 repetitions.
(2) rice seedling blight is tested:Experiment sets 4 processing altogether:①1×106Spore/g pythium oligandrum wettable powders
6000 times of liquid (preparation dosage 0.5g/m 2, to water 3kg), (May 5) even spraying is found the needle phase in rice, and seedbed is sprayed thoroughly,
In the 2nd same dosage spray of damping-off big generation phase (corresponding rice two leaf stage, May 12);②1×106Spore/g is few male rotten
Mould 3000 times of liquid of wettable powder (preparation dosage 1.0g/m2, to water 3kg), the same processing of spraying time, number and method is 1.;
(3. preparation dosage is 1mL/m to 35% 2000 times of liquid of first frost thiram wettable powder of comparison medicament2, to water 2kg), dispenser
The same processing of time, number and method is 1.;4. clear water compares (CK), the same processing of spraying time, number and method is 1..Respectively handle
Each secondary dispenser carries out on seedbed.3 repetitions are often handled, are included 5 disk rice shoots (reversed six ribs alms bowl disk, the cm of 58cm × 29).
The random district's groups arrangement in minizone.
(3) rice blast is tested:Experiment sets 4 processing altogether:①1×106Spore/g pythium oligandrum wettable powders
6000 times of liquid (preparation dosage 0.5g/m 2, to water 3kg), during entire experiment dispenser twice, respectively boot stage and Qi Sui
Phase.The position of emphasis spray is the top of plant.②1×106Spore/3000 times of liquid of g pythium oligandrums wettable powder (use by preparation
Measure 1.0g/m2, to water 3kg), the same processing of spraying time, number and method is 1..3. comparison medicament, 40% Isoprothiolane EC 1000
Times liquid, spraying time, number and method with processing 1..4. clear water compares (CK), the same processing of spraying time, number and method is 1..
3 repetitions are each handled, altogether 12 cells, plot area 25m2, the random district's groups arrangement of cell.
In rice seedling blight occurrence in peak period investigation it is each processing seedling-cultivation plate rice shoot occur damping-off, often repeatedly with
Machine takes 5 cave rice strains, and record total strain number, diseased plant number calculate the control effect of incidence and reagent agent to rice disease.
Diseased plant rate %=diseased plant numbers/investigation total strain number * 100
Control effect (%)=(control incidence-processing incidence)/control incidence × 100
Here is result statistical conditions after present invention experiment:
As shown in Table 1, either rice transplanting phase or heading stage, with 1 × 106Spore/g pythium oligandrum wettables
Pulvis application concentration increases, and bakanae disease of rice incidence is in rising trend, and control effect is on a declining curve.But the medicament 6000
The processing of times liquid is compared with 3000 times of liquid handle preventive effect without significant difference.In addition, with 3000 times of 25% Prochloraz missible oil of comparison medicament
Liquid processing is compared, the preventive effect at rice transplanting phase and heading stage there are no significant difference.In this experiment, microbial bactericide 1
×106Spore/6000 times of liquid of g pythium oligandrums wettable powder are best to the preventive effect of bakanae disease of rice, with common fungicide 25%
3000 times of liquid preventive effects of Prochloraz missible oil are suitable.
The processing of 1 different agents of table is to the control effect of seedbed bakanae disease of rice
As shown in Table 2, with 1 × 106Spore/g pythium oligandrum wettable powders application concentration increases, rice seedling blight
Incidence is in rising trend, and control effect is on a declining curve.6000 times of liquid processing of the medicament are compared with 3000 times of liquid handle preventive effect
No significant difference (being respectively 95.04% and 93.80%), and the two is compared with other processing, either rice shoot incidence are still
Control effect, all there are pole significant differences.The incidence of 2000 times of liquid processing of wherein 35% first frost thiram wettable powder
Up to 22.33%, control effect is only 45.09%.In this experiment, microbial bactericide 1 × 106Spore/g pythium oligandrums
The each concentration processing of wettable powder is significantly better than 35% first frost good fortune of chemical bactericide to the preventive effect performance of rice seedling blight
2000 times of liquid of U.S. double wettable powders, and it is best with the effect of its 6000 times of liquid.
The processing of 2 different agents of table is to the control effect of seedbed rice seedling blight
As shown in Table 3,6000 times of liquid of pythium oligandrum wettable powder, 3000 times of liquid, 40% 1000 times of Isoprothiolane EC liquid,
Three kinds of different processing have Rice Leaf rice blast certain preventive effect, wherein 6000 times of liquid of pythium oligandrum wettable powder,
40% 1000 times of Isoprothiolane EC liquid is above the preventive effect of Rice Leaf rice blast 3000 times of liquid of pythium oligandrum wettable powder, and
There was no significant difference for the preventive effect of the two, illustrates, 6000 times of liquid of pythium oligandrum wettable powder imitate the prevention of Rice Leaf rice blast
Fruit is worked as with 1000 times of liquid phases of common 40% Isoprothiolane EC of medicament.
The processing of 3 different agents of table is to the control effect of Rice Leaf rice blast
As shown in Table 4,6000 times of liquid of pythium oligandrum wettable powder, 3000 times of liquid, 40% 1000 times of Isoprothiolane EC liquid,
Three kinds of different processing have Rice Panicle rice blast certain preventive effect, and the preventive effect of three there are no significant difference, and by table
4 it is found that after 6000 times of liquid processing rice of pythium oligandrum wettable powder, and the incidence of Rice Panicle rice blast is less than pythium oligandrum
3000 times of liquid processing of wettable powder, it follows that 6000 times of liquid of pythium oligandrum wettable powder are to Rice Panicle rice blast
Control effect is worked as with 1000 times of liquid phases of common 40% Isoprothiolane EC of medicament.
The processing of 4 different agents of table is to the control effect of Rice Panicle rice blast
The above is only several embodiments of the present invention, any type of limitation is not done to the present invention, although this
Invention is disclosed as above with preferred embodiment, however not to limit the present invention, any person skilled in the art,
In the range of not departing from technical solution of the present invention, make a little variation using the technology contents of the disclosure above or modification is impartial
Equivalence enforcement case is same as, is belonged in technical solution of the present invention protection domain.