CN108152228A - A kind of method that biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed - Google Patents
A kind of method that biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed Download PDFInfo
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- CN108152228A CN108152228A CN201711290188.8A CN201711290188A CN108152228A CN 108152228 A CN108152228 A CN 108152228A CN 201711290188 A CN201711290188 A CN 201711290188A CN 108152228 A CN108152228 A CN 108152228A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses notoginseng planting technical fields, specific field is the method that a kind of biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed, 1) for the analysis of examination soil nutrient content, 2) selection and transplanting of biennial Radix Notoginseng seed item, 3) biennial Radix Notoginseng N fertilization, 4) measure of SPAD values, 5) management and 6) measure of nitrogen content and its leaf area, regression analysis is carried out according to the SPAD values under biennial Radix Notoginseng blade difference amount of nitrogen and corresponding unit leaf area nitrogen content (SLN) data and establishes equation of linear regression, so as to judge affiliated ranges of SPAD that Radix Notoginseng nitrogen is had more than needed, it is rapidly identified to have more than needed to biennial Radix Notoginseng leaf-nitrogen, so as to rationally scientifically apply fertilizer, final raising Radix Notoginseng plant deposits seedling rate, yield and quality.
Description
Technical field
The present invention relates to notoginseng planting technical field, specific field is rich for a kind of biennial Radix Notoginseng leaf-nitrogen of Rapid identification
Remaining method.
Background technology
Radix Notoginseng is Araliaceae Panax herbaceos perennial also known as invaluable, blood ginseng and pseudo-ginseng etc., with root and root-like stock
It is used as medicine, is China's tradition rare traditional Chinese medicine, in the course of cultivation, the cultivation to be standardized by the water and fertilizer management of science carries
High Radix Notoginseng plant deposits seedling rate, the important link of yield and quality.
Nitrogen is the required one of the chief elements of Panax notoginseng Growth development institute, and Radix Notoginseng has more than needed to nitrogen very sensitive, and nitrogen, which is had more than needed, to be made
Radix Notoginseng deposits the decline of seedling rate, and root tuber biomass is reduced, and the root rot incidence that rust crack mouth induces rises, and can reduce Rg1 saponin(es and contain
Amount, reduces total saponin content, and chlorophyll is that plant carries out photosynthetic main photosynthetic pigments, and content height can conduct
Plant physiology is studied and the important evidence of evaluation plant nitrogen nutrition situation, and chlorophyll content can be according to plant in different growths
SPECTRAL DIVERSITY under state is measured with chlorophyll meter, reflects leaf chlorophyll phase by sensing the difference of leaves of plants color value
To content, measurement result is known as SPAD values, has been widely used in the diagnosis and evaluation of crop nitrogen nutrition situation, the present invention
It is carried out according to the SPAD values under biennial Radix Notoginseng blade difference amount of nitrogen and corresponding unit leaf area nitrogen content (SLN) data
Regression analysis simultaneously establishes equation of linear regression, so as to judge affiliated ranges of SPAD that Radix Notoginseng nitrogen is had more than needed, so as to biennial three
Seven leaf-nitrogens are had more than needed and are rapidly identified, so as to rationally scientifically apply fertilizer.
Invention content
The purpose of the present invention is to provide the method that a kind of biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed, in solution
State the problem of being proposed in background technology.
To achieve the above object, the present invention provides following technical solution：A kind of biennial Radix Notoginseng leaf-nitrogen of Rapid identification
Method more than needed, method include the following steps：
1) for the analysis of examination soil nutrient content：To soil with organic matter content, total nitrogen content, ammonium nitrogen content, quick-acting
Phosphorus content, content of tatal phosphorus, quick-acting potassium content, full potassium content and soil pH value are measured；
2) selection and transplanting of biennial Radix Notoginseng seed item：Selection is without rust, robust growth and uniform biennial three
Seven seed items, it is 16~17cm to transplant in back cut diameter, and base diameter is 10~12cm, in the flowerpot of a height of 13~15cm, is placed in
In the shade of 8%~12% light transmittance, the processing of low nitrogen, middle nitrogen and the fertilising of high nitrogen is carried out after the emergence of seed item；
3) biennial Radix Notoginseng N fertilization：The application of nitrogenous fertilizer is divided into 4 periods, respectively April, June, July
With August part, low nitrogen processing is according to 0kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio of O is applied fertilizer, at middle nitrogen
Reason is according to 38.64kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, the processing of high nitrogen according to
77.28kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O；
4) measure of SPAD values：The biennial sanchi leaf handled using SPAD-502 Chlorophyll meters nitrogen in step 3)
The SPAD values of piece are measured；
5) it manages：Biennial Radix Notoginseng water regime is checked in fine day, and moisturizing is carried out using the spray irrigation system installed in greenhouse,
Soil moisture is maintained at 25%~30%；Rainy day notices seeing whether that, there are sufferer Radix Notoginseng, discovery has sufferer Radix Notoginseng to remove in time；
6) measure of nitrogen content and its leaf area：Leaf total nitrogen content is measured using Kjeldahl's method, each processing takes 6
Then the average value of~8 replication values is surveyed the leaf area of respective vanes with the portable leaf area instrument of LI-3000C
It is fixed.
Preferably, soil organic matter content described in step 1) is measured by organic matter photometry, and the total soil nitrogen contains
Amount is measured by azotometer, and the soil ammonium content is measured by spectrophotometry, the soil quick-effective phosphor and the soil
Earth content of tatal phosphorus is measured by molybdenum antimony resistance colorimetric method, and the Soil Available potassium content and the total potassium content of soil pass through flame light
Degree method measures, and the P in soil H values are counted by PH and measured.
Preferably, biennial Radix Notoginseng seed transplanting time described in step 2) is in January.
Preferably, the minute of step 4) the SPAD values is in July.
The beneficial effects of the invention are as follows：A kind of method that biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed, according to 2 years
SPAD values and corresponding unit leaf area nitrogen content (SLN) data under raw sangqi ginseng blade difference amount of nitrogen carry out regression analysis
And equation of linear regression is established, so as to judge affiliated ranges of SPAD that Radix Notoginseng nitrogen is had more than needed, so as to biennial Radix Notoginseng Leaf N
Element is more than needed rapidly to be identified, so as to rationally scientifically apply fertilizer, final raising Radix Notoginseng plant deposits seedling rate, yield and quality.
Description of the drawings
Fig. 1 is the relationship of chlorophyll content of the present invention (SPAD) and unit leaf area nitrogen content (SLN)；
Fig. 2 is the death rate that the present invention is high, neutralizes biennial Radix Notoginseng under low nitrogen processing.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme of the present invention is clearly and completely described, it is clear that institute
The embodiment of description is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Those of ordinary skill in the art's all other embodiments obtained without making creative work, belong to this hair
The range of bright protection.
Embodiment 1：
1) for the analysis of examination soil nutrient content：To soil with organic matter content, total nitrogen content, ammonium nitrogen content, quick-acting
Phosphorus content, content of tatal phosphorus, quick-acting potassium content, full potassium content and soil pH value are measured；
2) selection and transplanting of biennial Radix Notoginseng seed item：Selection is without rust, robust growth and uniform biennial three
Seven seed items, it is 16cm, base diameter 10cm to transplant in back cut diameter, in the flowerpot of a height of 13cm, is placed in the screening of 8% light transmittance
In cloudy canopy, the processing of low nitrogen, middle nitrogen and the fertilising of high nitrogen is carried out after the emergence of seed item；
3) biennial Radix Notoginseng N fertilization：The application of nitrogenous fertilizer is divided into 4 periods, respectively April, June, July
With August part, low nitrogen processing is according to 0kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio of O is applied fertilizer, at middle nitrogen
Reason is according to 38.64kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, the processing of high nitrogen according to
77.28kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O；
4) measure of SPAD values：The biennial sanchi leaf handled using SPAD-502 Chlorophyll meters nitrogen in step 3)
The SPAD values of piece are measured；
5) it manages：Biennial Radix Notoginseng water regime is checked in fine day, and moisturizing is carried out using the spray irrigation system installed in greenhouse,
Soil moisture is maintained at 25%~30%；Rainy day notices seeing whether that, there are sufferer Radix Notoginseng, discovery has sufferer Radix Notoginseng to remove in time；
6) measure of nitrogen content and its leaf area：Leaf total nitrogen content is measured using Kjeldahl's method, each processing takes 6
Then the average value of a replication value is measured the leaf area of respective vanes with the portable leaf area instrument of LI-3000C.
Embodiment 2：
1) for the analysis of examination soil nutrient content：To soil with organic matter content, total nitrogen content, ammonium nitrogen content, quick-acting
Phosphorus content, content of tatal phosphorus, quick-acting potassium content, full potassium content and soil pH value are measured；
2) selection and transplanting of biennial Radix Notoginseng seed item：Selection is without rust, robust growth and uniform biennial three
Seven seed items, it is 16.5cm, base diameter 11cm to transplant in back cut diameter, in the flowerpot of a height of 14cm, is placed in 10% light transmittance
Shade in, seed item emergence after carry out low nitrogen, middle nitrogen and high nitrogen fertilising processing；
3) biennial Radix Notoginseng N fertilization：The application of nitrogenous fertilizer is divided into 4 periods, respectively April, June, July
With August part, low nitrogen processing is according to 0kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio of O is applied fertilizer, at middle nitrogen
Reason is according to 38.64kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, the processing of high nitrogen according to
77.28kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O；
4) measure of SPAD values：The biennial sanchi leaf handled using SPAD-502 Chlorophyll meters nitrogen in step 3)
The SPAD values of piece are measured；
5) it manages：Biennial Radix Notoginseng water regime is checked in fine day, and moisturizing is carried out using the spray irrigation system installed in greenhouse,
Soil moisture is maintained at 25%~30%；Rainy day notices seeing whether that, there are sufferer Radix Notoginseng, discovery has sufferer Radix Notoginseng to remove in time；
6) measure of nitrogen content and its leaf area：Leaf total nitrogen content is measured using Kjeldahl's method, each processing takes 7
Then the average value of a replication value is measured the leaf area of respective vanes with the portable leaf area instrument of LI-3000C.
Embodiment 3：
1) for the analysis of examination soil nutrient content：To soil with organic matter content, total nitrogen content, ammonium nitrogen content, quick-acting
Phosphorus content, content of tatal phosphorus, quick-acting potassium content, full potassium content and soil pH value are measured；
2) selection and transplanting of biennial Radix Notoginseng seed item：Selection is without rust, robust growth and uniform biennial three
Seven seed items, it is 17cm, base diameter 12cm to transplant in back cut diameter, in the flowerpot of a height of 15cm, is placed in 12% light transmittance
In shade, the processing of low nitrogen, middle nitrogen and the fertilising of high nitrogen is carried out after the emergence of seed item；
3) biennial Radix Notoginseng N fertilization：The application of nitrogenous fertilizer is divided into 4 periods, respectively April, June, July
With August part, low nitrogen processing is according to 0kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio of O is applied fertilizer, at middle nitrogen
Reason is according to 38.64kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, the processing of high nitrogen according to
77.28kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O；
4) measure of SPAD values：The biennial sanchi leaf handled using SPAD-502 Chlorophyll meters nitrogen in step 3)
The SPAD values of piece are measured；
5) it manages：Biennial Radix Notoginseng water regime is checked in fine day, and moisturizing is carried out using the spray irrigation system installed in greenhouse,
Soil moisture is maintained at 25%~30%；Rainy day notices seeing whether that, there are sufferer Radix Notoginseng, discovery has sufferer Radix Notoginseng to remove in time；
6) measure of nitrogen content and its leaf area：Leaf total nitrogen content is measured using Kjeldahl's method, each processing takes 8
Then the average value of a replication value is measured the leaf area of respective vanes with the portable leaf area instrument of LI-3000C.
SPAD values and corresponding unit leaf area nitrogen content (SLN) under the different amount of nitrogens that Examples 1 to 3 is measured
Data carry out regression analysis and establish equation of linear regression, the affiliated ranges of SPAD that Radix Notoginseng nitrogen is had more than needed are judged, to biennial three
Seven leaf-nitrogens are had more than needed and are rapidly identified, according to qualification result, (the SPAD values that nitrogen is had more than needed are more than 60；Normally apply the three of nitrogen
Seven SPAD values are between 50~60) adjustment amount of application of nitrogen fertilizer, in final Examples 1 to 3 Radix Notoginseng plant deposit seedling rate, yield and
Quality is superior to existing Radix Notoginseng yield and quality.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of method that biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed, it is characterised in that：Its method includes the following steps：
1) for the analysis of examination soil nutrient content：Soil with organic matter content, total nitrogen content, ammonium nitrogen content, rapid available phosphorus are contained
Amount, content of tatal phosphorus, quick-acting potassium content, full potassium content and soil pH value are measured；
2) selection and transplanting of biennial Radix Notoginseng seed item：Selection is without rust, robust growth and uniform biennial Radix Notoginseng seed
Item, it is 16~17cm to transplant in back cut diameter, and base diameter is 10~12cm, in the flowerpot of a height of 13~15cm, it is placed in 8%~
In the shade of 12% light transmittance, the processing of low nitrogen, middle nitrogen and the fertilising of high nitrogen is carried out after the emergence of seed item；
3) biennial Radix Notoginseng N fertilization：The application of nitrogenous fertilizer is divided into 4 periods, respectively April, June, July and August
Part, low nitrogen processing is according to 0kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, middle nitrogen processing are pressed
According to 38.64kg/hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O, high nitrogen processing is according to 77.28kg/
hm2Purity nitrogen, 64.87kg/hm2P2O5, 47.65kg/hm2k2The ratio fertilising of O；
4) measure of SPAD values：Using SPAD-502 Chlorophyll meters to the biennial Radix Notoginseng blade of nitrogen processing in step 3)
SPAD values are measured；
5) it manages：Biennial Radix Notoginseng water regime is checked in fine day, and moisturizing, soil are carried out using the spray irrigation system installed in greenhouse
Humidity is maintained at 25%~30%；Rainy day notices seeing whether that, there are sufferer Radix Notoginseng, discovery has sufferer Radix Notoginseng to remove in time；
6) measure of nitrogen content and its leaf area：Leaf total nitrogen content is measured using Kjeldahl's method, each processing takes 6~8
Then the average value of a replication value is measured the leaf area of respective vanes with the portable leaf area instrument of LI-3000C.
2. the method that the biennial Radix Notoginseng leaf-nitrogen of a kind of Rapid identification according to claim 1 is had more than needed, it is characterised in that：
Soil organic matter content described in step 1) is measured by organic matter photometry, and the total nitrogen content of soil is surveyed by azotometer
Fixed, the soil ammonium content is measured by spectrophotometry, and the soil quick-effective phosphor and the Soil total nitrogen pass through
Molybdenum antimony resistance colorimetric method measures, and the Soil Available potassium content and the total potassium content of soil are described by flame spectrophotometric determination
P in soil H values are counted by PH and measured.
3. the method that the biennial Radix Notoginseng leaf-nitrogen of a kind of Rapid identification according to claim 1 is had more than needed, it is characterised in that：
Biennial Radix Notoginseng seed transplanting time described in step 2) is in January.
4. the method that the biennial Radix Notoginseng leaf-nitrogen of a kind of Rapid identification according to claim 1 is had more than needed, it is characterised in that：
The minute of step 4) the SPAD values is in July.
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| CN201711290188.8A CN108152228A (en) | 2017-12-08 | 2017-12-08 | A kind of method that biennial Radix Notoginseng leaf-nitrogen of Rapid identification is had more than needed |
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Cited By (2)
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| CN113109272A (en) * | 2021-04-20 | 2021-07-13 | 石河子大学 | Drip irrigation cotton nitrogen nutrition deficiency diagnosis method and system |
| CN114711010A (en) * | 2022-06-09 | 2022-07-08 | 苏州农业职业技术学院 | Water-soil fertilizer management method, system and medium in Chinese rose cultivation |
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| CN114711010B (en) * | 2022-06-09 | 2022-09-02 | 苏州农业职业技术学院 | Water-soil fertilizer management method, system and medium in Chinese rose cultivation |
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