CN108143754A - The application of asian tetracera root or leaf and its extract in terms for the treatment of and/or prevention tumour medicine is prepared - Google Patents
The application of asian tetracera root or leaf and its extract in terms for the treatment of and/or prevention tumour medicine is prepared Download PDFInfo
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- CN108143754A CN108143754A CN201810026373.4A CN201810026373A CN108143754A CN 108143754 A CN108143754 A CN 108143754A CN 201810026373 A CN201810026373 A CN 201810026373A CN 108143754 A CN108143754 A CN 108143754A
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- leaf
- asian tetracera
- extract
- tetracera root
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The present invention relates to medicinal application field, particularly asian tetracera root or leafs and its extract to prepare treatment and/or the application of prevention tumour medicine.Asian tetracera root or leaf extract of the present invention is selected from following any one or more mixing：Asian tetracera root or leaf water extract, asian tetracera root or leaf alcohol extracting thing, asian tetracera root or leaf ethyl acetate extract, asian tetracera root or leaf n-butanol extract.Asian tetracera root or leaf alcohol extracting thing provided by the invention is substantially better than other types extract to the inhibiting effect of human glioma cell.Asian tetracera root or leaf water extract provided by the invention is substantially better than other types extract to the inhibiting effect of human cervical carcinoma cell, human gastric adenocarcinoma.
Description
Technical field
The present invention relates to medicinal application field, particularly asian tetracera root or leafs and its extract to prepare treatment and/or pre- preventing tumor
The application of drug.
Background technology
Asian tetracera root or leaf is the root of Dilleniaceae asian tetracera root or leaf Tetracera asiatica (Lour.) Hoogland.Its is cool in nature, taste
Hardship has astringing to arrest diarrhea, the work(of swelling and pain relieving.It is usually used in treating the diseases such as diarrhea, dysentery, rectal prolapse.Rich in tannin, Huang in its medicinal material
The multiple biological activities ingredients such as ketone, terpene class substance have apparent antibacterial, anti-inflammatory, antitumor isoreactivity [national traditional Chinese medical science pencil
Book on Chinese herbal medicine editorial board China of China of reason office book on Chinese herbal medicine [M] Shanghai of volume 3：Shanghai science tech publishing house, 1999：510-511.].
Asian tetracera root or leaf herb resource enriches, and south China is produced with southwestern each province, is one of civil common Chinese herbal medicine.
Chinese patent application if publication No. is CN106236795A discloses a kind of Chinese medicine preparation with bacteriostasis,
A kind of particularly extraction with asian tetracera root or leaf (root of Dilleniaceae asian tetracera root or leaf Tetracera asiatica (Lour.) Hoogland)
Chinese medicine preparation made of object；Result of study shows that the Chinese medicine preparation can be to Hayes shigella dysenteriae, escherichia coli, golden yellow Portugal
Grape coccus, bacillus subtilis and typhoid bacillus have bacteriostatic activity effect, show good inhibiting bacteria and diminishing inflammation effect.
At present there has been no asian tetracera root or leaf and its extract in the report for preparing treatment and/or the application of prevention tumour medicine.
Invention content
In order to overcome the shortcomings of the prior art, the present invention provide asian tetracera root or leaf and its extract prepare treatment and/or
Prevent the application of tumour medicine.
Asian tetracera root or leaf of the present invention is Dilleniaceae asian tetracera root or leaf Tetracera asiatica (Lour.) Hoogland
Root.
The purpose of the present invention can be achieved through the following technical solutions：
Asian tetracera root or leaf is preparing treatment and/or the application of prevention tumour medicine.
Preferably, asian tetracera root or leaf is taken, solubilizer extraction obtains asian tetracera root or leaf extract.
Preferably, the asian tetracera root or leaf extract is selected from following any one or more mixing：Asian tetracera root or leaf water extract,
Asian tetracera root or leaf alcohol extracting thing, asian tetracera root or leaf ethyl acetate extract, asian tetracera root or leaf n-butanol extract.
Preferably, the asian tetracera root or leaf water extract is made by following methods：It takes asian tetracera root or leaf and crushes, press solid-liquid ratio 1 every time:
20~30 add in purified waters, 90~110 DEG C decoct 1~2h, repeat 2~4 times, merge to get.
Preferably, the asian tetracera root or leaf alcohol extracting thing is made by following methods：It takes asian tetracera root or leaf and crushes, press solid-liquid ratio 1 every time:
20~30 add in the ethyl alcohol that percent by volume is 40~60%, in 70~90 DEG C of water-bath 1~2h of refluxing extraction, repeat 2~4 times,
Merge to get.
Preferably, the asian tetracera root or leaf ethyl acetate extract is made by following methods：Take the asian tetracera root or leaf alcohol extracting thing point
It dissipates in the ethyl alcohol for being 40~60% in percent by volume, with ethyl acetate continuous extraction 10~20 times, 40~60mL every time, second
Acetoacetic ester extract liquor merge to get.
Preferably, the asian tetracera root or leaf n-butanol extract is made by following methods：The asian tetracera root or leaf alcohol extracting thing is taken to disperse
In the ethyl alcohol for being 40~60% in percent by volume, with ethyl acetate continuous extraction 10~20 times, 40~60mL every time, acetic acid
Ethyl ester extract liquor is cast out；Residue n-butanol continuous extraction 10~20 times, every time 40~60mL, butanol extraction liquid merge,
To obtain the final product.
Preferably, the asian tetracera root or leaf water extract is made by following methods：It takes asian tetracera root or leaf and crushes, press solid-liquid ratio 1 every time:
25 add in purified waters, 100 DEG C decoct 1h, be repeated 3 times, merge to get.
Preferably, the asian tetracera root or leaf alcohol extracting thing is made by following methods：It takes asian tetracera root or leaf and crushes, press solid-liquid ratio 1 every time:
25 add in percents by volume be 50% ethyl alcohol, in 80 DEG C of water-bath refluxing extraction 1h, be repeated 3 times, merge to get.
Preferably, the asian tetracera root or leaf ethyl acetate extract is made by following methods：Take the asian tetracera root or leaf alcohol extracting thing point
It dissipates in the ethyl alcohol for being 50% in percent by volume, with ethyl acetate continuous extraction 15 times, each 50mL, acetic acid ethyl acetate extract closes
And to get.
Preferably, the asian tetracera root or leaf n-butanol extract is made by following methods：The asian tetracera root or leaf alcohol extracting thing is taken to disperse
In the ethyl alcohol for being 50% in percent by volume, with ethyl acetate continuous extraction 15 times, each 50mL, acetic acid ethyl acetate extract house
It goes；Residue n-butanol continuous extraction 15 times, each 50mL, butanol extraction liquid merge to get.
Preferably, the tumour includes cervical carcinoma, glioma, gastric cancer.
The present invention also provides a kind of pharmaceutical composition, described pharmaceutical composition by the asian tetracera root or leaf with it is pharmaceutically acceptable
Complementary ingredient or therapeutic ingredient compatibility form.
The present invention also provides a kind of pharmaceutical composition, described pharmaceutical composition is by any asian tetracera root or leaf extract and pharmacy
Upper acceptable complementary ingredient or therapeutic ingredient compatibility form.
Compared with the prior art, the method have the advantages that：
1. asian tetracera root or leaf extract provided by the invention is to human cervical carcinoma cell Hela, human gastric adenocarcinoma BGC-823, people
Glioma U251 cells are respectively provided with apparent inhibiting effect, can be efficiently applied to the treatment and/or prevention of more than tumour.
2. asian tetracera root or leaf alcohol extracting thing provided by the invention is substantially better than other to the inhibiting effect of human glioma cell
Type-collection object.As activity >=80mg/L of asian tetracera root or leaf alcohol extracting thing, to the inhibiting effect effect of human glioma with
Cis-platinum maintains an equal level even better than cis-platinum.
3. asian tetracera root or leaf water extract provided by the invention is apparent to the inhibiting effect of human cervical carcinoma cell, human gastric adenocarcinoma
Better than other types extract.When activity >=160mg/L of asian tetracera root or leaf water extract, the inhibition to human gastric adenocarcinoma
Function and effect and cis-platinum maintain an equal level even better than cis-platinum；As activity >=400mg/L of asian tetracera root or leaf water extract, to human cervical carcinoma
The inhibiting effect effect of cell and cis-platinum maintain an equal level even better than cis-platinum.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The preparation of 1 asian tetracera root or leaf water extract of embodiment
The asian tetracera root or leaf medicinal powder 200g after crushing is taken, adds in 5000mL pure water every time, 1h is decocted at 100 DEG C, is repeated 3 times, close
And 3 gained extracting solutions, water extract medicinal extract is condensed into, it is 7.0771g that every 1g water extracts medicinal extract is made, which to contain crude drug amount,.
The preparation of 2 asian tetracera root or leaf alcohol extracting thing of embodiment
The asian tetracera root or leaf medicinal powder 100g after crushing is taken, adds in 2500mL, 50% ethyl alcohol, in 80 DEG C of water-bath refluxing extractions every time
1h is repeated 3 times, and is merged 3 gained extracting solutions, is concentrated to give asian tetracera root or leaf ethanol extract medicinal extract, makes 1g alcohol extracting things medicinal extract containing life
Dose is 6.9638g.
The preparation of embodiment 3 asian tetracera root or leaf ethyl acetate extract, n-butanol extract
2 gained alcohol extracting thing medicinal extract of Example, is scattered in 50% ethyl alcohol, with ethyl acetate continuous extraction 15 times, often
Secondary 50mL, extract liquor merge, and obtain asian tetracera root or leaf ethyl acetate extract medicinal extract；Residue n-butanol continuous extraction 15 times, every time
50mL, extract liquor merge, and obtain asian tetracera root or leaf n-butanol extract medicinal extract.Then in 80 DEG C of concentrations, soak 1g ethyl acetate extracts
Cream is 52.0833g containing crude drug amount, and it is 41.6667g that 1g n-butanol extracts medicinal extract is made, which to contain crude drug amount,.
Embodiment 4 is to human cervical carcinoma cell Hela, human gastric adenocarcinoma BGC-823, human glioma U251 cells
Inhibiting effect is tested
(1) experiment material：
Human cervical carcinoma cell Hela, human gastric adenocarcinoma BGC-823, human glioma's U251 cells：It is purchased from marine
The academy of sciences of state cell bank.Condition of culture：By cell culture in dual anti-containing 10% high-quality fetal calf serum and 1% Pen .- Strep
DMEM culture mediums in, 2-3d changes liquid 1 time, and when 80% degrees of fusion of cell passes on, can be according to 1：3 passage ratios carry out, in 37 DEG C,
5%CO2And it is cultivated in saturated humidity incubator.
Water extract mother liquor and dilution：1 gained water extract medicinal extract 100mg of embodiment is weighed, is dissolved in 1mLDMSO, mistake
The mother liquor that bacterium is formulated as 100mg/mL is filtered out, 4 DEG C of refrigerators is placed in and saves backup.Used time is diluted to 10 with DMSO respectively, 20,
40、80、160、400、600、800mg/L。
Alcohol extracting thing mother liquor and dilution：2 gained alcohol extract medicinal extract 100mg of embodiment is weighed, is dissolved in 1mL DMSO,
Filtration sterilization is to be formulated as the mother liquor of 100mg/mL, is placed in 4 DEG C of refrigerators and saves backup.Used time is diluted to 10 with DMSO respectively, 20,
40、80、160、400、600、800mg/L。
Ethyl acetate extract mother liquor and dilution：Embodiment 3 gained ethyl acetate extract medicinal extract 100mg is weighed, it is molten
In 1mL DMSO, filtration sterilization is to be formulated as the mother liquor of 100mg/mL, is placed in 4 DEG C of refrigerators and saves backup.Used time is with DMSO points
Be not diluted to 10,20,40,80,160,400,600,800mg/L.
N-butanol extract mother liquor and dilution：3 gained n-butanol extract medicinal extract 100mg of embodiment is weighed, is dissolved in
In 1mL DMSO, filtration sterilization is to be formulated as the mother liquor of 100mg/mL, is placed in 4 DEG C of refrigerators and saves backup.Used time is distinguished with DMSO
Be diluted to 10,20,40,80,160,400,600,800mg/L.
Cis-platinum mother liquor and dilution：It weighs cis-platinum (Qilu Pharmaceutical Co., Ltd.) 25mg and is dissolved in 8.33mL DMSO
In, filtration sterilization is formulated as 3mg/mL mother liquors, is placed in 4 DEG C of refrigerators and saves backup.Used time is diluted to 60ug/mL with DMSO.
(2) experimental method：
1) above-mentioned three kinds of tumour cells are taken respectively, count adjustment cell concentration to 1 × 105/mL。
2) it is inoculated in respectively in 96 orifice plates, per 100 μ L of hole, then 96 orifice plates is moved into incubator and are cultivated, are incubated overnight,
So that cell is completely adherent.
3) next day, will be diluted to 10,20,40,80,160,400,600, the water extract dilution of 800mg/L, alcohol extracting
Object dilution, ethyl acetate extract dilution, n-butanol extract dilute and the cis-platinum dilution of 60ug/mL is added to 96
In orifice plate corresponding aperture, per 10 μ L of hole, each 3 multiple holes of sample.
4) while normal group (untreated cell, culture medium) and blank group (culture medium) are set, then cell exists
48h is incubated in incubator.
5) the CCK-8 solution of 10 μ L is then added in per hole, in being incubated 1-4h in incubator.
6) the absorbance OD values at 450nm are measured with microplate reader.
And survival rate of the drug to cell is calculated according to the following formula.
Survival rate=[(A-X)/(A-A0)] × 100%
Wherein, A represents the absorbance value of blank group, A0The mean absorbance values normally organized are represented, X represents drug-treated
The mean absorbance values of group.Finally, it is fitted growth inhibition curve using 5.0 softwares of Graphpad Prism and calculates IC50Value.
(3) experimental result
1 various asian tetracera root or leaf extracts of table are to the function influence of human cervical carcinoma cell Hela survival rates
2 various asian tetracera root or leaf extracts of table are to the function influence of human gastric adenocarcinoma BGC-823 survival rates
3 various asian tetracera root or leaf extracts of table are to the function influence of human glioma's U251 cell survival rates
By table 1~3 it is found that as activity >=80mg/L of asian tetracera root or leaf alcohol extracting thing, the inhibition to human glioma
Function and effect and cis-platinum maintain an equal level even better than cis-platinum；As activity >=160mg/L of asian tetracera root or leaf water extract, to people's sdenocarcinoma of stomach
The inhibiting effect effect of cell and cis-platinum maintain an equal level even better than cis-platinum；As activity >=400mg/L of asian tetracera root or leaf water extract,
Maintain an equal level to the inhibiting effect effect of human cervical carcinoma cell and cis-platinum even better than cis-platinum.
4 various asian tetracera root or leaf extracts of table are to the IC of various tumour cells50
As shown in Table 4, asian tetracera root or leaf alcohol extracting thing provided by the invention is apparent to the inhibiting effect of human glioma cell
Better than other types extract；Suppression of the asian tetracera root or leaf water extract provided by the invention to human cervical carcinoma cell, human gastric adenocarcinoma
It makes of being substantially better than other types extract.
Although above having used general explanation, specific embodiment and experiment, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (10)
1. asian tetracera root or leaf is preparing treatment and/or the application of prevention tumour medicine.
2. application according to claim 1, which is characterized in that take asian tetracera root or leaf, solubilizer extraction obtains asian tetracera root or leaf extract.
3. application according to claim 2, which is characterized in that the asian tetracera root or leaf extract is selected from following any or a kind of
Above mixing：Asian tetracera root or leaf water extract, asian tetracera root or leaf alcohol extracting thing, asian tetracera root or leaf ethyl acetate extract, asian tetracera root or leaf n-butanol carry
Take object.
4. application according to claim 3, which is characterized in that the asian tetracera root or leaf water extract is made by following methods：It takes
Asian tetracera root or leaf simultaneously crushes, and presses solid-liquid ratio 1 every time:20~30 add in purified water, decoct 1~2h at 90~110 DEG C, repeat 2~4 times,
Merge to get.
5. application according to claim 3, which is characterized in that the asian tetracera root or leaf alcohol extracting thing is made by following methods：It takes
Asian tetracera root or leaf simultaneously crushes, and presses solid-liquid ratio 1 every time:20~30 add in the ethyl alcohol that percent by volume is 40~60%, in 70~90 DEG C of water
Bathe 1~2h of refluxing extraction, repeat 2~4 times, merge to get.
6. application according to claim 5, which is characterized in that the asian tetracera root or leaf ethyl acetate extract is by following methods system
：The asian tetracera root or leaf alcohol extracting thing is taken to be scattered in the ethyl alcohol that percent by volume is 40~60%, with ethyl acetate continuous extraction
10~20 times, every time 40~60mL, acetic acid ethyl acetate extract merge to get.
7. application according to claim 5, which is characterized in that the asian tetracera root or leaf n-butanol extract is by following methods system
：The asian tetracera root or leaf alcohol extracting thing is taken to be scattered in the ethyl alcohol that percent by volume is 40~60%, with ethyl acetate continuous extraction
10~20 times, 40~60mL, acetic acid ethyl acetate extract are cast out every time；Residue n-butanol continuous extraction 10~20 times, every time
40~60mL, butanol extraction liquid merge to get.
8. according to any application of claim 2~7, which is characterized in that the tumour includes cervical carcinoma, neuroglia
Knurl, gastric cancer.
9. a kind of pharmaceutical composition, which is characterized in that described pharmaceutical composition asian tetracera root or leaf and pharmaceutically may be used as described in claim 1
The complementary ingredient or therapeutic ingredient compatibility received forms.
10. a kind of pharmaceutical composition, which is characterized in that described pharmaceutical composition is carried by any asian tetracera root or leaf of claim 2~7
Object is taken to be formed with pharmaceutically acceptable complementary ingredient or therapeutic ingredient compatibility.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810026373.4A CN108143754B (en) | 2018-01-11 | 2018-01-11 | Application of cassytha leaves and extract thereof in preparing medicine for treating and/or preventing tumor |
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| CN201810026373.4A CN108143754B (en) | 2018-01-11 | 2018-01-11 | Application of cassytha leaves and extract thereof in preparing medicine for treating and/or preventing tumor |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014158685A2 (en) * | 2013-03-14 | 2014-10-02 | Avon Products, Inc | Tetracera asiatica extracts and methods of use |
| CN105012713A (en) * | 2015-08-13 | 2015-11-04 | 李荣勤 | Preparation for treating cervical cancer and preparation method thereof |
| CN106236795A (en) * | 2016-09-23 | 2016-12-21 | 广西中医药大学 | A kind of Chinese medicine preparation with bacteriostasis and production method |
-
2018
- 2018-01-11 CN CN201810026373.4A patent/CN108143754B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014158685A2 (en) * | 2013-03-14 | 2014-10-02 | Avon Products, Inc | Tetracera asiatica extracts and methods of use |
| CN105012713A (en) * | 2015-08-13 | 2015-11-04 | 李荣勤 | Preparation for treating cervical cancer and preparation method thereof |
| CN106236795A (en) * | 2016-09-23 | 2016-12-21 | 广西中医药大学 | A kind of Chinese medicine preparation with bacteriostasis and production method |
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| Title |
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| RAJAGOPAL SUBRAMANYAM等: ""Novel binding studies of human serum albumin with trans-feruloyl maslinic acid"", 《JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B: BIOLOGY》 * |
| 周兴栋: ""锡叶藤的化学成分研究"", 《中草药》 * |
| 季宇斌等主编: "《中药抗肿瘤有效成分药理与应用》", 31 May 1998, 黑龙江科学技术出版社 * |
| 朱华等: ""壮药锡叶藤茎总黄酮提取工艺研究及其不同产地的含量测定"", 《中国医院药学杂志》 * |
| 谢国材等编著: "《南方百草良方（下）》", 31 October 2008, 汕头大学出版社 * |
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