CN107951928A - The new opplication of Lotus Plumule P.E. - Google Patents
The new opplication of Lotus Plumule P.E. Download PDFInfo
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- CN107951928A CN107951928A CN201711381420.9A CN201711381420A CN107951928A CN 107951928 A CN107951928 A CN 107951928A CN 201711381420 A CN201711381420 A CN 201711381420A CN 107951928 A CN107951928 A CN 107951928A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The application belongs to field of medicaments, discloses the application of the new opplication of Lotus Plumule P.E., especially Lotus Plumule P.E. in the suppression medicine of bacterial community induction system is prepared.Experiment shows that Lotus Plumule P.E. is respectively provided with inhibition for the biomembrane of three kinds of pseudomonas aeruginosas, show that Lotus Plumule P.E. of the present invention can reach the growth that can not inhibited bacteria while bacterial virulence and pathogenicity is reduced by the intervention school-based inhibited bacteria, be not likely to produce the effect of bacterial drug resistance.Particularly described lotus nut chloroform layer extract, lotus nut ethyl acetate layer extract, Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone are respectively provided with more suitable than positive drug furan ketone compound or more preferable inhibition for the biomembrane of pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa, indicate Lotus Plumule P.E. of the present invention and are respectively provided with good inhibition for pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa.
Description
Technical field
The invention belongs to field of medicaments, and in particular to the new opplication of Lotus Plumule P.E., especially Lotus Plumule P.E. exist
Prepare the application suppressed in medicine of bacterial community induction system.
Background technology
With the extensive use of antibiotic, the drug resistance intensity of microorganism is higher and higher, Antibiotic Resistance is more and more wider, drug resistance shape
Into speed rising proportional to antibiotic sterilization capability.Drug resistance once produces, it will keeps.Antibiotic continues to make
With can be only that high antibody-resistant bacterium continue to provide selection pressure, promote it to replicate, group and enjoys drug resistant gene at structure jointly, cause more
The quickening of weight antibody-resistant bacterium is formed.At present, antibiotic resistance has become the serious public health problem in the whole world.
China causes 80,000,000,000 yuan of medical expenses to increase because of abuse of antibiotics every year, while causes 80,000 patients bad anti-because of its
Should death.No matter illness weight, operation size at present, all using antibiotic and tending to high-titer antibiotic and often
A large amount of blindness long-time drug combinations, even use more than 4 kinds of antibiotic and cause new antibody-resistant bacterium in a short time individually
Continuously emerge.Some experts even predict that China may take the lead in entering " rear antibiotic epoch ", that is, return to before antibiotic is found when
Generation.
China possesses extremely abundant Chinese medicine and natural pharmaceutical resources, and nearly ten thousand kinds of medicinal plant, provides for new drug discovery
Abundant material base and source.Moreover, carrying out antibacterial, anti-inflammatory with Chinese patent drug substitute antibiotics, there is adverse reaction and secondary work
With it is relatively small, do not produce the advantages that drug resistance.On the other hand, world medical circle does not have real meaning in the time 40 years existing
On new antibiotic be born.Therefore, many researchers focus on crude drug " antibiotic " in spite of oneself
On.Being found from traditional Chinese medicine resource equally has the alternative product of antibiotic of antibacterial anti-inflammatory curative effect, can solve to resist well
Raw this world-famous puzzle of element drug resistance.
In recent years, bacterial community sensing (Quorum sensing, QS) system becomes studies new drug-resistance bacteria medicine
Important target.The inhibitory action mechanism of bacterium widely studied at present is mainly by inhibiting bacteria DNA gyrases, inhibiting bacteria
The function of cytoplasma membrane, inhibit bacteria energetic supersession etc. performance bacteriostasis efficacy.And bacterial community sensing and the suppression of bacterium
The mechanism of action is different.Bacterial community sensing (Quorumsensing, QS) is that an intercellular signal dependent on bacterial density passes
Guiding systems, bacterial community behavior is critically adjusted in a manner of chemistry exchanges, including bacterial biof iotalm is formed, virulence factor base
Because of the regulation and control of expression, the generation of drug resistance, bacteria motility and cometabolism.Pass through suppression by target of bacterial community induction system
Its ability of regulation and control and then reduce the expression of the related virulence gene of its regulation and control and the pathogenecity that inhibits bacteria, can reduce it is thin
The growth not inhibited bacteria while bacterium power and pathogenicity, selection will not be produced compared with conventional antibiotic to the existence of bacterium
Property pressure, is not likely to produce bacterial drug resistance in theory.QS is in bacterial cell or a kind of mode of intercellular signal transmission, passes through
Some signaling molecules (also known as autoinducer) are monitored such as homoserine lactone (acyl-homoserine lactone, AHL)
Concentration, to control and coordinate whole bacterial community behavior, surrounding environment is stimulated jointly and is made a response, is greatly enhanced whole
The survival ability of bacterial community.
Lotus nut (Plumula nelumbinis), also known as the lotus heart of a lotus seed, the bitter heart of a lotus seed, lotus nut, see Tang end earliest《Feeding habits sheet
Grass》In, it is the drying plumule in the perennial water plant lotus mature seed of Nymphaeceae.Its main place of production be distributed in China Hunan,
Hubei, Fujian, Jiangsu, Zhejiang, Jiangxi etc. save.Lotus nut is cold in nature, bitter, the thoughts of returning home, kidney channel, has clearing away the heart fire and tranquillizing, restoring normal coordination between heart and kidney
And the effect of unsmoothing the sperm and stopping bleeding, for treating the diseases such as the invasion of pericardium by heat, coma and delirium, breakdown of the normal physiological coordination between the heart and the kidney, insomnia seminal emission and blood-head haematemesis, it is
One of common Chinese medicine with " clearing heat and detoxicating " effect.Lotus nut chemical composition includes：Double benzyl tetrahydro isoquinoline class biologies
Alkali, monobenzyl tetrahydroisoquinoline alkaloid, flavonoids, sterol and organic acid, volatile oil etc., wherein with lotus nut bases
Double benzyl tetrahydro isoquinoline Alkaloids based on compound are the characteristic chemical constituent of lotus nut and its content is higher.System reviews lotus
The document of the sub- heart finds, modern pharmacology and clinical research also indicate that lotus nut to pseudomonas aeruginosa, bacillus salmonella, golden yellow
The common bacterias such as color staphylococcus, Escherichia coli, bacillus subtilis are respectively provided with stronger inhibitory action, but do not find it
Inhibit bacteria the document report of intervention school-based.
A variety of chemical compositions, such as alkaloids, flavonoids, volatile oil, organic acid are included in Lotus Plumule P.E., at present
Not yet find that it inhibits bacteria intervention school-based.
The content of the invention
In view of this, the present invention provides the new opplication of Lotus Plumule P.E., i.e. Lotus Plumule P.E. to prepare bacterial flora
The application suppressed in medicine of body induction system.
Pseudomonas aeruginosa (P.aeruginosa) has the very strong ability that biomembrane is formed in tissue surface, and its
QS system researches obtain also the most thoroughly, therefore pseudomonas aeruginosa is chosen as pattern bacterium.Pseudomonas aeruginosa is a kind of important
Conditioned pathogen, usually causes the inside-hospital infections such as respiratory tract infection, pneumonia, urethral infection, it is considered to be patient is in hospital
The third-largest pathogenic bacteria that period infects, seriously endanger the health and life of the mankind.The high inherence of pseudomonas aeruginosa resists
Pharmacological property is inseparable with its intervention school-based, which, which controls, includes biomembrane, exotoxin, elastoser, haemolysis
The expression of nearly all virulence factor such as element, pyo.These virulence factors determine cause of the pseudomonas aeruginosa to host
Sick ability.Wherein, biomembrane formation and diffusion be to cause an important mechanisms of P.aeruginosa multidrug resistants.State of the U.S.
The authoritative survey report of portion of vertical Institutes of Health Research (NIH) issue points out, human microbial is infected with being by thin more than 80%
Bacterium biofilm (Biofilm, BF) mediation.As a kind of bacterial community behavior, it breaks up and development and bacterial flora body-sensing BF
Should be closely related.The complete bacterium of bacterial community induction system can form development and break up normal, typical can resist and kill
The biomembrane of microbial inoculum, and the bacterium of bacterial community induction system incompleteness cannot then form typical biomembrane, and antibiotic is supported
Drag is remarkably decreased, and is easily rinsed and sensitive to fungicide.Therefore, formed by being quenched control bacterial biof iotalm
, will because not inhibiting bacteria growth directly, selection pressure will not be produced to bacterium with the QS systems of pathogen virulence factor expression
Get a good chance of obtaining the group's sense inhibitor (QS inhibitors, QSI) for acting on novel targets, bacterium will not being made to produce drug resistance.
The present invention carries out biomembrane Inhibition test using pseudomonas aeruginosa as research object.The results show lotus nut carries
Take lotus nut ethanol primary extract, Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone, the lotus nut chloroform layer in thing
Extract, lotus nut ethyl acetate layer extract, lotus nut n-butanol layer extract, lotus nut water layer extract are false for verdigris
Monad 9027, the biomembrane of three kinds of pseudomonas aeruginosas of pseudomonas aeruginosa 27853 and tolerant Pseudomonas aeruginosa are respectively provided with
Inhibition, showing that Lotus Plumule P.E. of the present invention can be reached by the intervention school-based inhibited bacteria can drop
The growth not inhibited bacteria while low bacterial virulence and pathogenicity, is not likely to produce the effect of bacterial drug resistance.I.e. described lotus seeds
Heart extract can play resisting pseudomonas aeruginosa by suppressing pseudomonas aeruginosa and/or tolerant Pseudomonas aeruginosa biomembrane
And/or the effect of tolerant Pseudomonas aeruginosa.
Therefore, the answering in the suppression medicine of bacterial community induction system is prepared the present invention provides Lotus Plumule P.E.
With.
Wherein, Lotus Plumule P.E. of the present invention is lotus nut ethanol primary extract, Plumula nelumbinis Alkaloid extract, lotus
Sub- heart extractive of general flavone, lotus nut chloroform layer extract, lotus nut ethyl acetate layer extract, the extraction of lotus nut n-butanol layer
At least one of thing, lotus nut water layer extract.
Preferably, the lotus nut ethanol primary extract is 60% ethanol primary extract of lotus nut and/or 80% second of lotus nut
Alcohol primary extract.
Preferably, the preparation method of the Lotus Plumule P.E. includes the following steps：
(1) take the drying herb of lotus nut to crush, add aqueous solvent refluxing extraction, lotus seeds are dried to obtain after removing insoluble matter
Heart ethanol primary extract；
(2) lotus nut primary extract is taken, after adding distilled water dissolving, sequentially adds isometric chloroform, ethyl acetate, positive fourth
Alcoholic solvent carries out extraction and respectively obtains lotus nut chloroform layer extract, lotus nut ethyl acetate layer extract, lotus nut n-butanol
Layer extract, lotus nut water layer extract；
(3) lotus nut primary extract is taken, after adding 0.1%HCl dissolvings, is loaded into pretreated D001 cation exchange trees
Fat, carries out gradient with the ethanol water of water, percent by volume 0%, 10%, 30%, 50%, 70% and 95% successively and washes
It is de-, obtain lotus nut water lotion, the elution fraction of 0% ethanol of lotus nut, elution fraction, the lotus nut of 10% ethanol of lotus nut
The elution fraction of 30% ethanol, the elution fraction of 50% ethanol of lotus nut, the elution fraction and lotus nut of 70% ethanol of lotus nut
The elution fraction of 95% ethanol；Wherein, the elution fraction of 70%~95% ethanol is Plumula nelumbinis Alkaloid extract；
(4) after obtained lotus nut water lotion is concentrated, pretreated AB-8 macroporous absorbent resins is loaded into, are used successively
Water, the ethanol water of percent by volume 0%, 20%, 40%, 60%, 80% and 100% carry out gradient elution, obtain lotus
The elution fraction of sub- 0% ethanol of the heart, the elution fraction of 20% ethanol of lotus nut, elution fraction, the lotus seeds of 40% ethanol of lotus nut
The elution fraction of the elution fraction of 60% ethanol of the heart, 100% ethanol of elution fraction and lotus nut of 80% ethanol of lotus nut；Its
In, the elution fraction of 80%~100% ethanol is lotus nut extractive of general flavone.
Preferably, the aqueous solvent is water or ethanol water.
Preferably, the removal insoluble matter is specially collected by filtration filtrate or supernatant is collected by centrifugation.
Preferably, the refluxing extraction is thermal extraction or cold extraction.It will be understood by those skilled in the art that the reflux
Extraction includes but not limited to the methods of cold soaking extraction, leakage extraction, surname extraction and circumfluence distillation.
Preferably, the addition of the aqueous solvent is 8~15 times of lotus nut weight, Extracting temperature is 10~150
DEG C, extraction time is 2~6 times, when each extraction time is 1~72 small.
In some embodiments, 60% second for adding 60% alcohol reflux and being extracted as adding 10 times of lotus nut weight
Alcohol reflux extract 3 times, Extracting temperature be 130 DEG C, every time 2 it is small when, merge extracting solution.
In some embodiments, 80% second for adding 80% alcohol reflux and being extracted as adding 12 times of lotus nut weight
Alcohol reflux extract 3 times, Extracting temperature be 120 DEG C, every time 2 it is small when, merge extracting solution.
Preferably, the drying is to be dried under reduced pressure.
Preferably, the extraction extracts three times respectively for chloroform, ethyl acetate and water-saturated n-butanol.
The step of concentration being further included after the extraction.Preferably, the concentration is to be concentrated under reduced pressure.
Further, biomembrane inhibition assay result is shown, the lotus nut chloroform layer extract, lotus nut ethyl acetate
Layer extract, Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone are false for pseudomonas aeruginosa and drug resistance verdigris
The biomembrane of monad is respectively provided with more suitable than positive drug furan ketone compound or more preferable inhibition.Therefore, preferably,
Lotus Plumule P.E. of the present invention is lotus nut chloroform layer extract, lotus nut ethyl acetate layer extract, lotus nut are always given birth to
Alkaloids extract, lotus nut extractive of general flavone.
Preferably, the bacterium is pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa.
Preferably, medicine of the present invention includes the Lotus Plumule P.E. of effective dose.
Preferably, medicine of the present invention further includes pharmaceutically acceptable auxiliary material.
Those skilled in the art can prepare the Lotus Plumule P.E. direct or indirect addition required during different dosage forms
Pharmaceutically acceptable various common auxiliary materials, such as filler, disintegrant, lubricant, adhesive, with traditional drug formulations side
Method, is made common oral formulations or ejection preparation.
Preferably, the oral formulations are tablet, capsule, granule.
Preferably, the ejection preparation is parenteral solution or powder-injection.
In some embodiments, the medicine is resisting pseudomonas aeruginosa granule, is made of following raw material：
10 parts of lotus nut n-butanol layer extract, 15 parts of lactose, 3 parts of 10% starch slurry.
In some embodiments, the medicine is resisting pseudomonas aeruginosa capsule, is made of following raw material：
20 parts of Plumula nelumbinis Alkaloid extract, 30 parts of lactose, 30 parts of microcrystalline cellulose, 3 parts of talcum powder.
In some embodiments, the medicine is overriding resistance pseudomonas aeruginosa tablet, by following raw material system
Into：12 parts of lotus nut extractive of general flavone, 15 parts of lactose, 5 parts of 10% starch slurry, 8 parts of crospovidone, 2 parts of magnesium stearate.
As shown from the above technical solution, the present invention provides the new opplication of Lotus Plumule P.E., especially lotus nut to extract
Application of the thing in the suppression medicine of bacterial community induction system is prepared.Experiment shows Lotus Plumule P.E. for P. aeruginosa
Bacterium 9027, the biomembrane of three kinds of pseudomonas aeruginosas of pseudomonas aeruginosa 27853 and tolerant Pseudomonas aeruginosa are respectively provided with suppression
Effect, showing that Lotus Plumule P.E. of the present invention can be reached by the intervention school-based inhibited bacteria can reduce carefully
The growth not inhibited bacteria while bacterium power and pathogenicity, is not likely to produce the effect of bacterial drug resistance.Particularly described lotus seeds
Heart chloroform layer extract, lotus nut ethyl acetate layer extract, Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone
For pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa biomembrane be respectively provided with than positive drug furan ketone compound quite or
More preferable inhibition, indicates Lotus Plumule P.E. of the present invention for pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa
It is respectively provided with good inhibition.
Embodiment
The invention discloses the new opplication of Lotus Plumule P.E..Those skilled in the art can use for reference present disclosure, suitably
Modified technique parameter is realized.In particular, all similar substitutions and modifications are for a person skilled in the art
It will be apparent that they are considered as being included in the present invention.The method and product of the present invention is carried out by preferred embodiment
Description, related personnel can substantially not depart from present invention, method described herein are being modified in spirit and scope
Or suitably change with combining, to realize and using the technology of the present invention.
For a further understanding of the present invention, below in conjunction with the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that and described embodiment is only part of the embodiment of the present invention, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, belongs to the scope of protection of the invention.
Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can pass through business canal
Road purchase obtains.
The preparation of embodiment 1,80% ethanol primary extract of lotus nut and total alkaloid
(1) lotus nut (Plumula Nelambinis) 10kg, with 12 times amount 80% (v/v) ethanol soaked overnights after plus
Circumfluence distillation 3 times, Extracting temperature be 120 DEG C, every time 2 it is small when, merge extracting solution；
(2) insoluble matter is filtered to remove, filtrate decompression drying, obtains 80% ethanol primary extract 2.5kg of lotus nut；
(3) after 80% ethanol primary extract 2.3kg of lotus nut being added appropriate 0.1%HCl dissolvings, it is loaded into pretreated
D001 cation exchange resins.(contain 1%NH with pure water, 95% ethanol solution successively3) elution, obtain water lotion 700g and 95%
Alcohol washing lotion 850g.Wherein, 95% alcohol washing lotion (contains 1%NH3) in be mainly enriched alkaloid compound, i.e. lotus nut is total
Alkaloid extract.
The preparation of embodiment 2,60% ethanol primary extract of lotus nut and general flavone
(1) lotus nut (Plumula Nelambinis) 5kg, with 10 times amount 60% (v/v) ethanol soaked overnights after plus
Circumfluence distillation 3 times, Extracting temperature be 130 DEG C, every time 2 it is small when, merge extracting solution；
(2) insoluble matter is filtered to remove, filtrate decompression drying, obtains 60% ethanol primary extract 1.6kg of lotus nut；
(3) after 60% ethanol primary extract 1.5kg of lotus nut being added appropriate 0.1%HCl dissolvings, it is loaded into pretreated
D001 cation exchange resins.(contain 1%NH with pure water, 95% ethanol solution successively3) elution, obtain water lotion 500g and 95%
Alcohol washing lotion 350g.
(4) by obtained water lotion 450g after being concentrated under reduced pressure, pretreated AB-8 macroporous absorbent resins are loaded into, according to
It is secondary to be eluted with pure water, 95% ethanol solution, obtain the alcohol washing lotion 210g of water lotion 60g and 95%.Wherein, in 95% alcohol washing lotion
Mainly it is enriched flavonoids class compound, i.e. lotus nut extractive of general flavone.
The preparation of embodiment 3,80% ethanol primary extract of lotus nut and each separation component
(1) lotus nut (Plumula Nelambinis) 8kg, with 10 times amount 80% (v/v) ethanol soaked overnights after plus
Circumfluence distillation 3 times, Extracting temperature be 120 DEG C, every time 2 it is small when, merge extracting solution；
(2) filtering removes insoluble matter, is dried under reduced pressure, obtains 80% ethanol primary extract 2.1kg of lotus nut；
(3) 80% ethanol primary extract 2.0kg of lotus nut is distributed in water into the aqueous solution of 10L, with isometric chloroform,
Ethyl acetate and n-butanol extract three times respectively, are concentrated under reduced pressure to give lotus nut chloroform layer extract 230g, lotus nut acetic acid second
Ester layer extract 320g, lotus nut n-butanol layer extract 450g, lotus nut water layer extract 400g.
The biomembrane Inhibition test of embodiment 4, Lotus Plumule P.E.
Bacterium：Pseudomonas aeruginosa 9027 (ATCC 9027), pseudomonas aeruginosa 27853 (ATCC27853) and drug resistance copper
Green pseudomonad (persister).
Test compound：Using the bromo- 5- of furan ketone compound (Z) -4- (bromine methylene) -2 (5H)-furanones to be positive right
According to using DMSO as negative control, positive drug and Lotus Plumule P.E. of the present invention are configured to 32,64,128 μ g/ respectively
mL。
Experimental method：The compound to be tested that 100 μ L are prepared is separately added into orifice plate, is inoculated with 100 μ L bacterium solutions.Set
Blank control group (200 μ L of LB culture mediums) and negative control group (LB culture mediums and each 100 μ L of bacterium solution).It is placed in 37 DEG C of incubators and incubates
Change.After 20h, hole endosexine bacterium solution is drawn, distillation water washing three times, washes away planktonic bacteria.After dry or oven for drying, add
220 μ L concentration are 1% crystal violet, and room temperature places 30min, then distilled water carefully washing 3 times；Add 230 μ L, 95% ethanol
Dissolve biomembrane-crystal violet compound, measure orifice plate absorbance at microplate reader 630nm wavelength, parallel determination three times, the result is shown in
Table 1.
Inhibitory action of 1 Lotus Plumule P.E. of table for three kinds of aeruginosa biofilms
The inhibition assay result of above-mentioned three kinds of aeruginosa biofilms shows Lotus Plumule P.E. of the present invention
Various components are respectively provided with inhibition for the biomembrane of three kinds of pseudomonas aeruginosas, show that the Lotus Plumule P.E. of the present invention can
To reach what can be do not inhibited bacteria while bacterial virulence and pathogenicity is reduced by the intervention school-based inhibited bacteria
Grow, be not likely to produce the effect of bacterial drug resistance.Particularly lotus nut chloroform layer extract, the extraction of lotus nut ethyl acetate layer
Thing, Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone are for pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa
Biomembrane be respectively provided with inhibition quite more more preferable than positive drug furan ketone compound, indicate lotus nut of the present invention extraction
Thing is respectively provided with good inhibition for pseudomonas aeruginosa and tolerant Pseudomonas aeruginosa.
Embodiment 5,60% ethanol extract resisting pseudomonas aeruginosa granule of lotus nut
60% ethanol extract 15mg of lotus nut
Lactose 20mg；
10% starch slurry 4mg；
The preparation process of resisting pseudomonas aeruginosa granule is：
(1) lotus nut n-butanol layer extract first with lactose mixing 10-15 minutes；
(2) 10% starch slurry softwood is added, crosses 14 mesh sieves, it is dry after granulation；
(3) 12 mesh sieves are crossed, are dried after whole grain, up to resisting pseudomonas aeruginosa granule.
Embodiment 6,80% ethanol extract resisting pseudomonas aeruginosa capsule of lotus nut
The preparation process of resisting pseudomonas aeruginosa capsule is：
(1) 80% ethanol extract of lotus nut first with lactose mixing 5-15 minutes；
(2) mixed 5-15 minutes after adding microcrystalline cellulose；
(3) mixed 3-6 minutes after adding talcum powder；
(4) mixture loads gelatine capsule shell, up to resisting pseudomonas aeruginosa capsule.
Embodiment 7, lotus nut chloroform layer extract overriding resistance pseudomonas aeruginosa tablet
The preparation process of overriding resistance pseudomonas aeruginosa tablet is：
(1) lotus nut extractive of general flavone first with lactose mixing 5-15 minutes；
(2) 10% starch slurry softwood is added, crosses 14 mesh sieves, it is dry after granulation, cross 12 mesh sieve whole grains；
(3) and then crospovidone and magnesium stearate are added, mixed 3-6 minutes；
(4) tabletting after mixing, up to overriding resistance pseudomonas aeruginosa tablet.
Embodiment 8, lotus nut ethyl acetate layer extract resisting pseudomonas aeruginosa granule
Lotus nut ethyl acetate layer extract 20mg
Lactose 25mg；
10% starch slurry 5mg；
The preparation process of resisting pseudomonas aeruginosa granule is：
(1) lotus nut n-butanol layer extract first with lactose mixing 10-15 minutes；
(2) 10% starch slurry softwood is added, crosses 14 mesh sieves, it is dry after granulation；
(3) 12 mesh sieves are crossed, are dried after whole grain, up to resisting pseudomonas aeruginosa granule.
Embodiment 9, lotus nut n-butanol layer extract resisting pseudomonas aeruginosa granule
Lotus nut n-butanol layer extract 10mg
Lactose 15mg；
10% starch slurry 3mg；
The preparation process of resisting pseudomonas aeruginosa granule is：
(1) lotus nut n-butanol layer extract first with lactose mixing 10-15 minutes；
(2) 10% starch slurry softwood is added, crosses 14 mesh sieves, it is dry after granulation；
(3) 12 mesh sieves are crossed, are dried after whole grain, up to resisting pseudomonas aeruginosa granule.
Embodiment 10, lotus nut water layer extract resisting pseudomonas aeruginosa capsule
The preparation process of resisting pseudomonas aeruginosa capsule is：
(1) lotus nut water layer extract first with lactose mixing 5-15 minutes；
(2) mixed 5-15 minutes after adding microcrystalline cellulose；
(3) mixed 3-6 minutes after adding talcum powder；
(4) mixture loads gelatine capsule shell, up to resisting pseudomonas aeruginosa capsule.
Embodiment 11, resisting pseudomonas aeruginosa capsule
The preparation process of resisting pseudomonas aeruginosa capsule is：
(1) Plumula nelumbinis Alkaloid extract first with lactose mixing 5-15 minutes；
(2) mixed 5-15 minutes after adding microcrystalline cellulose；
(3) mixed 3-6 minutes after adding talcum powder；
(4) mixture loads gelatine capsule shell, up to resisting pseudomonas aeruginosa capsule.
Embodiment 12, overriding resistance pseudomonas aeruginosa tablet
The preparation process of overriding resistance pseudomonas aeruginosa tablet is：
(1) lotus nut extractive of general flavone first with lactose mixing 5-15 minutes；
(2) 10% starch slurry softwood is added, crosses 14 mesh sieves, it is dry after granulation, cross 12 mesh sieve whole grains；
(3) and then crospovidone and magnesium stearate are added, mixed 3-6 minutes；
(4) tabletting after mixing, up to overriding resistance pseudomonas aeruginosa tablet.
The biomembrane Inhibition test of embodiment 13, eight kind of preparation
It is prepared by 60% ethanol extract resisting pseudomonas aeruginosa granule of lotus nut, the Example 6 of the preparation of Example 5
80% ethanol extract resisting pseudomonas aeruginosa Capsule content of lotus nut, Example 7 prepare lotus nut chloroform layer
The anti-verdigris of lotus nut ethyl acetate layer extract prepared by extract overriding resistance pseudomonas aeruginosa tablet, Example 8 is false single
Lotus seeds edema with the heart involved prepared by born of the same parents bacterium granule, the lotus nut n-butanol layer extract granule of the preparation of Example 9, Example 10
Layer extract resisting pseudomonas aeruginosa Capsule content, 11 Plumula nelumbinis Alkaloid extract capsule agent content of Example
Lotus nut extractive of general flavone tablet made from thing, embodiment 12, tests its inhibitory activity to biomembrane respectively, specific test
For step with embodiment 4, test result is shown in Table 2.
Inhibitory action of 2 Lotus Plumule P.E. of table for three kinds of aeruginosa biofilms
The foregoing is merely the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, on the premise of raw material of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. application of the Lotus Plumule P.E. in the suppression medicine of bacterial community induction system is prepared.
2. application according to claim 1, it is characterised in that the Lotus Plumule P.E. for lotus nut ethanol primary extract,
Plumula nelumbinis Alkaloid extract, lotus nut extractive of general flavone, lotus nut chloroform layer extract, lotus nut ethyl acetate layer extraction
Take at least one of thing, lotus nut n-butanol layer extract, lotus nut water layer extract.
3. application according to claim 2, it is characterised in that the lotus nut ethanol primary extract is 60% ethanol of lotus nut
80% ethanol primary extract of primary extract and/or lotus nut.
4. application according to claim 1, it is characterised in that the preparation method of the Lotus Plumule P.E. includes following step
Suddenly：
(1) take the drying herb of lotus nut to crush, add aqueous solvent refluxing extraction, lotus nut second is dried to obtain after removing insoluble matter
Alcohol primary extract；
(2) lotus nut primary extract is taken, after adding distilled water dissolving, it is molten to sequentially add isometric chloroform, ethyl acetate, n-butanol
Agent carries out extraction and respectively obtains lotus nut chloroform layer extract, lotus nut ethyl acetate layer extract, lotus nut n-butanol layer extraction
Take thing, lotus nut water layer extract；
(3) lotus nut primary extract is taken, after adding 0.1%HCl dissolvings, is loaded into pretreated D001 cation exchange resins,
Gradient elution is carried out with the ethanol water of water, percent by volume 0%, 10%, 30%, 50%, 70% and 95% successively, is obtained
To lotus nut water lotion, the elution fraction of 0% ethanol of lotus nut, the elution fraction of 10% ethanol of lotus nut, 30% second of lotus nut
The elution fraction of alcohol, the elution fraction of 50% ethanol of lotus nut, 95% second of elution fraction and lotus nut of 70% ethanol of lotus nut
The elution fraction of alcohol；Wherein, the elution fraction of 70%~95% ethanol is Plumula nelumbinis Alkaloid extract；
(4) by obtained lotus nut water lotion concentrate after, be loaded into pretreated AB-8 macroporous absorbent resins, successively with water,
The ethanol water that percent by volume is 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution, obtains lotus nut
The elution fraction of 0% ethanol, the elution fraction of 20% ethanol of lotus nut, elution fraction, the lotus nut of 40% ethanol of lotus nut
The elution fraction of the elution fraction of 60% ethanol, 100% ethanol of elution fraction and lotus nut of 80% ethanol of lotus nut；Wherein,
The elution fraction of 80%~100% ethanol is lotus nut extractive of general flavone.
5. application according to claim 1, it is characterised in that the bacterium is false single for pseudomonas aeruginosa and drug resistance verdigris
Born of the same parents bacterium.
6. application according to claim 1, it is characterised in that the medicine includes the Lotus Plumule P.E. of effective dose.
7. application according to claim 5, it is characterised in that the medicine further includes pharmaceutically acceptable auxiliary material.
8. apply according to claim 5, it is characterised in that the medicine is oral formulations or ejection preparation.
9. apply according to claim 7, it is characterised in that the oral formulations are tablet, capsule, granule；It is described
Ejection preparation is parenteral solution or powder-injection.
10. application according to claim 1, it is characterised in that the medicine its by 10%-90% Lotus Plumule P.E.
Formed with the pharmaceutically acceptable auxiliary material of 10%-90%.
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| CN116942720A (en) * | 2023-09-21 | 2023-10-27 | 畜科生物工程有限公司 | Application of rhizoma Atractylodis extract in inhibiting bacterial biofilm and composition for inhibiting bacterial biofilm formation |
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| CN109589350A (en) * | 2019-02-15 | 2019-04-09 | 广东工业大学 | The new opplication of Lotus Plumule volatile oil |
| CN110786341A (en) * | 2019-11-15 | 2020-02-14 | 中南民族大学 | Preparation method and application of lotus plumule extracting solution |
| CN110786341B (en) * | 2019-11-15 | 2021-04-06 | 中南民族大学 | Preparation method and application of lotus plumule extracting solution |
| CN111714490A (en) * | 2020-07-20 | 2020-09-29 | 广东工业大学 | Application of compound in preparation of medicine for inhibiting bacterial quorum sensing system |
| CN111714490B (en) * | 2020-07-20 | 2021-07-06 | 广东工业大学 | Application of compound in preparation of medicine for inhibiting bacterial quorum sensing system |
| CN112641686A (en) * | 2020-12-24 | 2021-04-13 | 天津国瑞蓝天科技有限公司 | Plant extract composition with antibacterial and anti-inflammatory functions and preparation method thereof |
| CN112618561A (en) * | 2021-02-25 | 2021-04-09 | 成都大学 | Application of ribavirin in preparation of medicine for inhibiting bacterial quorum sensing |
| CN116942720A (en) * | 2023-09-21 | 2023-10-27 | 畜科生物工程有限公司 | Application of rhizoma Atractylodis extract in inhibiting bacterial biofilm and composition for inhibiting bacterial biofilm formation |
| CN116942720B (en) * | 2023-09-21 | 2023-12-15 | 畜科生物工程有限公司 | Application of rhizoma Atractylodis extract in inhibiting bacterial biofilm and composition for inhibiting bacterial biofilm formation |
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