CN108142639A - The preparation method of selenium-enriched earthworm albumen powder - Google Patents
The preparation method of selenium-enriched earthworm albumen powder Download PDFInfo
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- CN108142639A CN108142639A CN201711482584.0A CN201711482584A CN108142639A CN 108142639 A CN108142639 A CN 108142639A CN 201711482584 A CN201711482584 A CN 201711482584A CN 108142639 A CN108142639 A CN 108142639A
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- 241000361919 Metaphire sieboldi Species 0.000 title claims abstract description 218
- 239000000843 powder Substances 0.000 title claims abstract description 114
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 71
- 239000011669 selenium Substances 0.000 title claims abstract description 71
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 140
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 140
- 238000002156 mixing Methods 0.000 claims abstract description 61
- 241000209094 Oryza Species 0.000 claims abstract description 37
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 37
- 235000009566 rice Nutrition 0.000 claims abstract description 37
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 19
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 16
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 16
- 230000008569 process Effects 0.000 claims abstract description 16
- 239000012141 concentrate Substances 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 11
- 239000012043 crude product Substances 0.000 claims abstract description 10
- 230000004888 barrier function Effects 0.000 claims abstract description 8
- 230000000903 blocking effect Effects 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 74
- 239000002002 slurry Substances 0.000 claims description 49
- 238000012870 ammonium sulfate precipitation Methods 0.000 claims description 36
- 239000006228 supernatant Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000005119 centrifugation Methods 0.000 claims description 23
- 239000012528 membrane Substances 0.000 claims description 23
- 238000001914 filtration Methods 0.000 claims description 21
- 239000000919 ceramic Substances 0.000 claims description 18
- 239000012535 impurity Substances 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 13
- 230000000149 penetrating effect Effects 0.000 claims description 13
- 238000000926 separation method Methods 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 12
- 230000008859 change Effects 0.000 claims description 10
- 235000021110 pickles Nutrition 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000003925 fat Substances 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000002203 pretreatment Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 102000004882 Lipase Human genes 0.000 claims description 3
- 108090001060 Lipase Proteins 0.000 claims description 3
- 239000004367 Lipase Substances 0.000 claims description 3
- 102000005840 alpha-Galactosidase Human genes 0.000 claims description 3
- 108010030291 alpha-Galactosidase Proteins 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 235000014633 carbohydrates Nutrition 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 3
- 238000001976 enzyme digestion Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 235000019421 lipase Nutrition 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 239000012466 permeate Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 230000036541 health Effects 0.000 abstract description 3
- 238000005185 salting out Methods 0.000 abstract description 2
- 206010039921 Selenium deficiency Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 230000002429 anti-coagulating effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P30/00—Shaping or working of foodstuffs characterised by the process or apparatus
- A23P30/40—Foaming or whipping
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
A kind of preparation method of selenium-enriched earthworm albumen powder,Belong to the preparing technical field of selenium-enriched earthworm albumen powder,Earthworm is crushed crude product earthworm protein is extracted out of earthworm body using ammonium sulfate salting-out process first by this method,Again by crude product earthworm protein by digesting,The high earthworm protein concentrate of free from admixture purity is obtained after ultrafiltration step,And by earthworm protein concentrate it is freeze-dried after obtain earthworm protein powder,In order to improve the health value of earthworm protein powder,Therefore selenium-rich rice powder is added in earthworm protein powder,And earthworm protein powder and selenium-rich rice powder are placed in mixing device and are uniformly mixed,Barrel lenght direction is provided with several block baffles in mixing device,To form several independent activity spaces in mixing device,So as to which when cylinder rolls, baffle forms barrier force to earthworm protein powder and selenium-rich rice powder,Earthworm protein powder and selenium-rich rice powder scatter under the blocking of baffle,Achieve the purpose that earthworm protein powder and selenium-rich rice powder are uniformly mixed.
Description
Technical field
The invention belongs to the preparing technical fields of selenium-enriched earthworm albumen powder, and in particular to a kind of system of selenium-enriched earthworm albumen powder
Preparation Method.
Background technology
Research shows that selenium being unevenly distributed in natural environment, according to incompletely statistics, there are 42 countries in the whole world
Or regional selenium deficiency.There is also selenium poison areas and selenium deficiency area in China, most to lack selenium area for selenium deficiency, wherein being lacked in national 22 provinces
Selenium, Pin Xi counties are up to 72%, and nearly 700,000,000 population in area in China about 2/3 is in selenium deficiency state.Therefore exploitation Organic Selenium strengthens food
Product, it has also become the important content of China's se-rich health food products exploitation at present.
Earthworm also known as earthworm, earthworm dried protein content reach 70%, contain in earthworm protein there are many enzyme and trace element,
Wherein containing a small amount of selenium, the problems such as can softening blood vessel according to clinical test earthworm protein, improve blood vessel blockage, human body is improved
The anticoagulating activity of itself, can activate the differentiation characteristic of vascular endothelial cell, therefore be widely used in medical domain in recent years,
Disease is prevented and treated, while is also used in cosmetics, and earthworm protein also containing the substance for being capable of beauty in earthworm protein
With good health-care effect and without side-effects, therefore selenium element is filled into earthworm protein, to improve the health care of earthworm protein
Performance so as to provide a kind of product with more preferable health properties, has a vast market prospect.
Invention content
In view of this, it is necessary to which a kind of preparation method of the selenium-enriched earthworm albumen powder with good health-care effect is provided.
A kind of preparation method of selenium-enriched earthworm albumen powder, extraction step and selenium-enriched earthworm albumen powder including earthworm protein
Preparation process；
The extraction step of earthworm protein specifically includes the enzyme of earthworm pre-treatment step, earthworm liquid separation and Extraction step, earthworm protein
Solve the ultrafiltration step of step and earthworm protein；
Earthworm pre-treatment step specifically includes：
（1）Screening：Live body earthworm is sieved to remove earthworm excrement, soil and the other impurities in earthworm；
（2）Cleaning：Live body earthworm after screening is put into service sink, soaks cleaning, to remove the silt on earthworm body
Impurity；
（3）Homogenate：The earthworm cleaned up is put into colloid mill uniformly to crush and wears into paste, earthworm slurries are made；
（4）Precipitate silt and filtering：Earthworm slurries are placed in lautertuns and adds in pure water and is filtered, earthworm slurries and pure water
Additional proportion is 1:1~1:2, lautertuns is divided into the first lautertuns and the second lautertuns, in the first lautertuns and the second lautertuns
Inlet is provided with intake valve, and the pure air after filtering is squeezed by intake valve in lautertuns, in the first lautertuns and
Screen pipe is respectively arranged in two lautertuns, is uniformly provided with filter opening on screen pipe, earthworm slurries are by the suction of pump from mistake
Filter opening extraction on chimney filter, so as to fulfill the separation of earthworm slurries and large granular impurity；Specifically filtration step is：First earthworm is starched
Liquid is passed through to the first lautertuns, and the intake valve that opening is arranged on the first lautertuns inlet is ventilated into the first lautertuns, with
The dispersibility of earthworm slurries is improved, convenient for salt precipitation protein below, intake valve is then shut off, opens pump, pass through pump
By the earthworm slurries in the first lautertuns, the intake line out of pond is filtered suction, and the aperture of screen pipe is in the first lautertuns
5mm, while gauze is provided on screen pipe, the aperture of gauze is 90 mesh, to increase filter effect, is made miscellaneous in earthworm slurries
Matter is removed more thorough, to improve the purity of earthworm protein, is then passed to further filtering and impurity removing in the second lautertuns, the second mistake
The aperture of filter pocket interior conduit is 2mm, while the gauze aperture being arranged on the second lautertuns pipeline is 120 mesh, through the second filtering
Earthworm slurries after slot filtering wait for subsequent processing through pumped；Screen pipe in first lautertuns and the second lautertuns is using more
Pipeline sets to increase filter area and improve filter efficiency；
（5）Stored frozen：Earthworm slurries after filtering are put into storage barrel, are stored under the conditions of -15 DEG C ~ -20 DEG C, it is spare；
（6）It thaws：The earthworm of stored frozen is taken out and is thawed under the conditions of 4 DEG C；
Earthworm liquid separation and Extraction step specifically includes：
（1）Prepare liquid of saltouing for the first time：The ammonium sulfate precipitation liquid that saturation degree is 30% is prepared, specially：It is pre-chilled at 100 parts 4 degree
17.6 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added in pure water, makes solid ammonium sulfate fully molten
Solution obtains ammonium sulfate precipitation liquid；
（2）It saltouts for the first time：The ammonium sulfate precipitation liquid that the saturation degree prepared is taken to be 30% is slowly added into earthworm slurries, and
Constantly earthworm slurries are stirred during the addition process, after ammonium sulfate precipitation liquid has added in, ammonium sulfate precipitation will be added in
The earthworm slurries of liquid are stood overnight, so that the protein in earthworm slurries precipitates completely, earthworm slurries and ammonium sulfate precipitation liquid
Additional proportion is 1:5；
（3）It centrifuges for the first time：Earthworm slurries after saltouing overnight centrifuge 15min under the rotating speed of 14000r/min, and respectively
First time centrifugation and first time centrifuged supernatant are collected, first time centrifugation is stored under the conditions of -20 DEG C, to first
Secondary centrifuged supernatant carries out pickle change；
（4）Preparation is saltoutd liquid：The ammonium sulfate precipitation liquid that saturation degree is 20% is prepared, specially：In the pure water of 4 degree of precoolings at 100 parts
13.4 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added to, solid ammonium sulfate is made fully to dissolve to obtain
Ammonium sulfate precipitation liquid；
（5）Pickle change：Above-mentioned first time centrifuged supernatant under the conditions of 4 DEG C is pre-chilled, after precooling, takes the saturation prepared
The ammonium sulfate precipitation liquid spent for 20% is slowly added into first time centrifuged supernatant, and ammonium sulfate precipitation liquid with centrifuging for the first time
The adding proportion of clear liquid is 5:1, and constantly first time centrifuged supernatant is stirred in ammonium sulfate precipitation liquid adition process
It mixes, ammonium sulfate precipitations liquid is waited all to add in first time centrifuged supernatant, 1h is persistently stirred to first time centrifuged supernatant, it is quiet
It puts overnight；
（6）Second of centrifugation：Above-mentioned pickle change liquid is centrifuged into 15min, and collect second under the rotating speed of 14000r/min
Secondary centrifugation and second of centrifuged supernatant, second centrifuged supernatant is for fermentation fertilizer, second of centrifugation and the
Centrifugation set, obtains raw protein；
Raw protein is obtained into earthworm protein powder after enzymolysis step, ultrafiltration step again；
The preparation process of selenium-enriched earthworm albumen powder specifically includes：Selenium-rich rice powder, earthworm are added in the earthworm protein powder prepared
Earthworm albumen powder and the addition mass ratio of selenium-rich rice powder are 5:1, earthworm protein powder is mixed with selenium-rich rice powder with mixing device
It closes uniformly, obtains selenium-enriched earthworm albumen powder.
Preferably, the enzymolysis step of the earthworm protein specifically includes：Using food-grade NaOH by first time centrifugation
The raw protein obtained with second of centrifugation set adjusts pH to 8 ~ 9, then added in into raw protein 40PPm ~
The complex enzyme of 100PPm makes the temperature of raw protein be maintained at enzyme digestion reaction 10h ~ 14h at 40 DEG C ~ 50 DEG C, to remove crude product egg
Sugar and fat in white.
Preferably, the ultrafiltration step of earthworm protein specifically includes：（1）Ceramic membrane filter：In raw protein after enzymolysis
It adds in deionized water, and deionized water and raw protein is uniformly mixing to obtain raw protein liquid, then by charging pump by crude product
Protein liquid is squeezed into ceramic membranous system, and the circulating pump in ceramic membranous system is constantly run, and makes raw protein liquid in ceramic membranous system
Middle filtering, ceramic membrane filter aperture are 50nm, and the large granular impurity in raw protein liquid is removed, and obtain raw protein infiltration
Liquid, in 4m/s ~ 6m/s, cycle pump pressure is controlled in 0.2Mpa ~ 0.35Mpa for the crossflow velocity control of ceramic membrane；
（2）Ultrafiltration membrane concentrates：Raw protein penetrating fluid obtained above is squeezed into hyperfiltration membrane assembly, crude product after high-pressure pump is pressurized
Water in albumen penetrating fluid enters per-meate side through ultrafiltration membrane under high pressure, and the earthworm protein liquid in raw protein penetrating fluid is with height
Pressure flow is gone out, the molecular weight of ultrafiltration membrane retention for 5000 dalton, so as to reach in raw protein penetrating fluid earthworm protein and
The purpose of water separation, obtains earthworm protein concentrate；
（3）Freeze-drying：Above-mentioned earthworm protein concentrate is freeze-dried, it is earthworm protein powder to obtain powder product.
Preferably, the complex enzyme wherein in enzymolysis step includes alpha-galactosidase and lipase, can be by earthworm protein
In carbohydrate and fats degradation.
Preferably, the mixing device used in the preparation process of selenium-enriched earthworm albumen powder includes mixing machine, mixing machine water
Flat setting on the ground, inlet port and outlet port, the inclined receipts of inlet port and outlet port is respectively arranged at the both ends of mixing machine
Mouth shape, the bore of inlet port and outlet port are gradually reduced outward from mixing machine cylinder, and mixing machine lower end both sides are provided with drive
Dynamic device, mixing machine roll under the drive of driving device, and the earthworm protein powder in mixing machine and selenium-rich rice powder are mixed
Uniformly, several baffles are provided in mixing machine, the height of baffle is less than the radius of cylinder, and is uniformly provided on baffle
Mixing machine cylinder is divided into several independent activity spaces by micropore, baffle, so that baffle is to earthworm egg when mixing machine rolls
White powder and selenium-rich rice powder form barrier force, and earthworm protein powder and selenium-rich rice powder scatter under the blocking of baffle, a part
Earthworm protein powder and selenium-rich rice powder are fallen by the micropore on baffle, and another part earthworm protein powder and selenium-rich rice powder are being kept off
It turns back and falls under the barrier force of plate, so as to achieve the purpose that earthworm protein powder and selenium-rich rice powder are uniformly mixed.
Preferably, the baffle includes four, is respectively and vertically fixed at four, the upper and lower, left and right orientation of mixing machine cylinder
Middle part, the baffle be rectangle, the length of the baffle is identical with the length of mixing machine cylinder, and the width of the baffle is
The 1/2 of the mixing machine cylinder radius is provided with the supporting rib of vertical interlaced, to prevent baffle plate in the plane of the baffle
Deformed damage in mixing process due to the impact of albumen powder.
The present invention is using above-mentioned technical proposal, advantage：Earthworm is crushed utilize ammonium sulfate first by this method
Salting out method extracts crude product earthworm protein out of earthworm body, then crude product earthworm protein is obtained nothing after enzymolysis, ultrafiltration step
The high earthworm protein concentrate of impurity purity, and by earthworm protein concentrate it is freeze-dried after obtain earthworm protein powder, in order to
The health value of earthworm protein powder is improved, therefore selenium-rich rice powder is added in earthworm protein powder, and by earthworm protein powder and richness
Selenium rice meal is placed in mixing device and is uniformly mixed, and barrel lenght direction is provided with several block baffles in mixing device,
To form several independent activity spaces in mixing device, so that baffle is to earthworm protein powder and selenium-rich when cylinder rolls
Rice meal formed barrier force, earthworm protein powder and selenium-rich rice powder scatter under the blocking of baffle, reach earthworm protein powder and
The purpose that selenium-rich rice powder is uniformly mixed.
Description of the drawings
Fig. 1 is the structure diagram of the lautertuns of a better embodiment.
Fig. 2 is the structure diagram of the lautertuns of another angle of lautertuns in Fig. 1.
Fig. 3 is the structure diagram of the mixing device of a better embodiment.
Fig. 4 is the sectional view in A-A directions in Fig. 3.
Fig. 5 is the structure diagram of baffle in mixing device.
In figure：Lautertuns 10, the first lautertuns 11, screen pipe 111, gauze 112, intake valve 113, the 114, second filtering of pump
Slot 12；Mixing device 20, mixing machine 21, feed inlet 22, discharge port 23, driving device 24, baffle 25, rib 251.
Specific embodiment
An embodiment of the present invention provides a kind of preparation method of selenium-enriched earthworm albumen powder, the extraction step including earthworm protein
With the preparation process of selenium-enriched earthworm albumen powder；
The extraction step of earthworm protein specifically includes the enzyme of earthworm pre-treatment step, earthworm liquid separation and Extraction step, earthworm protein
Solve the ultrafiltration step of step and earthworm protein；
Earthworm pre-treatment step specifically includes：
（1）Screening：Live body earthworm is sieved to remove earthworm excrement, soil and the other impurities in earthworm；
（2）Cleaning：Live body earthworm after screening is put into service sink, soaks cleaning, to remove the silt on earthworm body
Impurity；
（3）Homogenate：The earthworm cleaned up is put into colloid mill uniformly to crush and wears into paste, earthworm slurries are made；
Referring also to Fig. 1, Fig. 2,（4）Precipitate silt and filtering：Earthworm slurries are placed in addition pure water in lautertuns 10 to carry out
The additional proportion of filter, earthworm slurries and pure water is 1:1~1:2, lautertuns 10 divides for the first lautertuns 11 and the second lautertuns 12,
The inlet of first lautertuns 11 and the second lautertuns 12 is provided with intake valve 113, by the pure air after filtering by into
Air valve 113 is squeezed into lautertuns, is respectively arranged with screen pipe 111 in the first lautertuns 11 and the second lautertuns 12, is being filtered
Filter opening is uniformly provided on pipe 111, earthworm slurries are extracted out by the suction of pump 114 from the filter opening on screen pipe 111, so as to fulfill
The separation of earthworm slurries and large granular impurity；Specifically filtration step is：First earthworm slurries are passed through to the first lautertuns 11, are beaten
It opens and is arranged on the intake valve 113 of 11 inlet of the first lautertuns and ventilates into the first lautertuns 11, to improve point of earthworm slurries
Property is dissipated, convenient for salt precipitation protein below, intake valve 113 is then shut off, opens pump 114, by the suction of pump 114 by the
Earthworm slurries in one lautertuns 11 intake line out of pond is filtered, and the aperture of screen pipe 111 is in the first lautertuns 11
5mm, while gauze 112 is provided on screen pipe 111, the aperture of gauze 112 is 90 mesh, to increase filter effect, makes earthworm
Impurity in slurries removes more thorough, to improve the purity of earthworm protein, then passes to further mistake in the second lautertuns 12
Filter out miscellaneous, the aperture of 12 interior conduit of the second lautertuns is 2mm, while 112 hole of gauze being arranged on 12 pipeline of the second lautertuns
Diameter is 120 mesh, and the earthworm slurries after the filtering of the second lautertuns 12 wait for subsequent processing through 114 extraction of pump；First lautertuns 11
Multi-pipeline setting is used with the screen pipe 111 in the second lautertuns 12 to increase filter area and improve filter efficiency；
（5）Stored frozen：Earthworm slurries after filtering are put into storage barrel, are stored under the conditions of -15 DEG C ~ -20 DEG C, it is spare；
（6）It thaws：The earthworm of stored frozen is taken out and is thawed under the conditions of 4 DEG C；
Earthworm liquid separation and Extraction step specifically includes：
（1）Preparation is saltoutd liquid：The ammonium sulfate precipitation liquid that saturation degree is 30% is prepared, specially：In the pure water of 4 degree of precoolings at 100 parts
17.6 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added to, solid ammonium sulfate is made fully to dissolve to obtain
Ammonium sulfate precipitation liquid；
（2）It saltouts for the first time：The ammonium sulfate precipitation liquid that the saturation degree prepared is taken to be 30% is slowly added into earthworm slurries, and
Constantly earthworm slurries are stirred during the addition process, after ammonium sulfate precipitation liquid has added in, ammonium sulfate precipitation will be added in
The earthworm slurries of liquid are stood overnight, so that the protein in earthworm slurries precipitates completely, earthworm slurries and ammonium sulfate precipitation liquid
Additional proportion is 1:5；
（3）It centrifuges for the first time：Earthworm slurries after saltouing overnight centrifuge 15min under the rotating speed of 14000r/min, and respectively
First time centrifugation and first time centrifuged supernatant are collected, first time centrifugation is stored under the conditions of -20 DEG C, to first
Secondary centrifuged supernatant carries out pickle change；
（4）Preparation is saltoutd liquid：The ammonium sulfate precipitation liquid that saturation degree is 20% is prepared, specially：In the pure water of 4 degree of precoolings at 100 parts
13.4 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added to, solid ammonium sulfate is made fully to dissolve to obtain
Ammonium sulfate precipitation liquid；Due to having contained ammonium sulfate in supernatant, thus pickle change use saturation degree for 20% ammonium sulfate
It saltouts liquid；
（5）Pickle change：Above-mentioned first time centrifuged supernatant under the conditions of 4 DEG C is pre-chilled, after precooling, takes the saturation prepared
The ammonium sulfate precipitation liquid spent for 20% is slowly added into first time centrifuged supernatant, and ammonium sulfate precipitation liquid with centrifuging for the first time
The adding proportion of clear liquid is 5:1, and constantly first time centrifuged supernatant is stirred in ammonium sulfate precipitation liquid adition process
It mixes, ammonium sulfate precipitations liquid is waited all to add in first time centrifuged supernatant, 1h is persistently stirred to first time centrifuged supernatant, it is quiet
It puts overnight；
（6）Second of centrifugation：Above-mentioned pickle change liquid is centrifuged into 15min, and collect second under the rotating speed of 14000r/min
Secondary centrifugation and second of centrifuged supernatant, second centrifuged supernatant is for fermentation fertilizer, second of centrifugation and the
Centrifugation set, obtains raw protein；
The preparation process of selenium-enriched earthworm albumen powder specifically includes：Selenium-rich rice powder, earthworm are added in the earthworm protein powder prepared
Earthworm albumen powder and the addition mass ratio of selenium-rich rice powder are 5:1, earthworm protein powder is mixed with selenium-rich rice powder with mixing device
It closes uniformly, obtains selenium-enriched earthworm albumen powder.
Further, the enzymolysis step of the earthworm protein specifically includes：First time is centrifuged using food-grade NaOH and is sunk
The raw protein obtained with second of centrifugation set that forms sediment adjusts pH to 8 ~ 9, then added in into raw protein 40PPm ~
The complex enzyme of 100PPm makes the temperature of raw protein be maintained at enzyme digestion reaction 10h ~ 14h at 40 DEG C ~ 50 DEG C, to remove crude product egg
Sugar and fat in white.
Further, the ultrafiltration step of earthworm protein specifically includes：（1）Ceramic membrane filter：Raw protein after enzymolysis
Middle addition deionized water, and deionized water and raw protein are uniformly mixing to obtain raw protein liquid, then will be thick by charging pump
Product protein liquid is squeezed into ceramic membranous system, and the circulating pump in ceramic membranous system is constantly run, and makes raw protein liquid in ceramic membrane system
It is filtered in system, ceramic membrane filter aperture is 50nm, and the large granular impurity in raw protein liquid is removed, raw protein is obtained and oozes
Transparent liquid, in 4m/s ~ 6m/s, cycle pump pressure is controlled in 0.2Mpa ~ 0.35Mpa for the crossflow velocity control of ceramic membrane；
（2）Ultrafiltration membrane concentrates：Raw protein penetrating fluid obtained above is squeezed into hyperfiltration membrane assembly, crude product after high-pressure pump is pressurized
Water in albumen penetrating fluid enters per-meate side through ultrafiltration membrane under high pressure, and the earthworm protein liquid in raw protein penetrating fluid is with height
Pressure flow is gone out, the molecular weight of ultrafiltration membrane retention for 5000 dalton, so as to reach in raw protein penetrating fluid earthworm protein and
The purpose of water separation, obtains earthworm protein concentrate；
（3）Freeze-drying：Above-mentioned earthworm protein concentrate is freeze-dried, it is earthworm protein powder to obtain powder product.
Further, the complex enzyme wherein in enzymolysis step includes alpha-galactosidase and lipase, can be by earthworm egg
Carbohydrate and fats degradation in white.
Referring also to Fig. 3 to Fig. 5, further, the mixing device used in the preparation process of selenium-enriched earthworm albumen powder
10 include mixing machine 11, and mixing machine 11 is horizontally disposed on the ground, and 12 He of feed inlet is respectively arranged at the both ends of mixing machine 11
The bore of discharge port 13,13 inclined closing in shape of feed inlet 12 and discharge port, feed inlet 12 and discharge port 13 is from mixing machine 11
Body is gradually reduced outward, 11 lower end both sides of mixing machine is provided with driving device 14, mixing machine 11 is in the band of driving device 14
Dynamic lower rolling, the earthworm protein powder in mixing machine 11 and selenium-rich rice powder is uniformly mixed, if being provided in mixing machine 11
Dry baffle 15, the height of baffle 15 is less than the radius of 11 cylinder of mixing machine, and micropore, baffle are uniformly provided on baffle 15
11 cylinder of mixing machine is divided into several independent activity spaces by 15, so that baffle 15 is to earthworm protein when mixing machine 11 rolls
Powder and selenium-rich rice powder form barrier force, and earthworm protein powder and selenium-rich rice powder scatter under the blocking of baffle 15, a part
Earthworm protein powder and selenium-rich rice powder are fallen by the micropore on baffle 15, and another part earthworm protein powder and selenium-rich rice powder exist
It turns back and falls under the barrier force of baffle, so as to achieve the purpose that earthworm protein powder and selenium-rich rice powder are uniformly mixed.
Further, the baffle 15 includes four, is respectively and vertically fixed at four, the upper and lower, left and right orientation of cylinder
Middle part, the baffle 15 are rectangle, and the length of the baffle is identical with the length of 11 cylinder of mixing machine, the width of the baffle 15
1/2 for the 11 cylinder radius of mixing machine is spent, the supporting rib of vertical interlaced is provided in the plane of the baffle 15
151, it is deformed in mixing process due to the impact of albumen powder damage to prevent baffle plate 15.
The above disclosure is only the preferred embodiments of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, one of ordinary skill in the art will appreciate that realizing all or part of flow of above-described embodiment, and will according to right of the present invention
Made equivalent variations are sought, still falls within and invents covered range.
Claims (6)
1. a kind of preparation method of selenium-enriched earthworm albumen powder, it is characterised in that：Extraction step and selenium-rich earthworm including earthworm protein
The preparation process of earthworm albumen powder；
The extraction step of earthworm protein specifically includes the enzyme of earthworm pre-treatment step, earthworm liquid separation and Extraction step, earthworm protein
Solve the ultrafiltration step of step and earthworm protein；
Earthworm pre-treatment step specifically includes：
（1）Screening：Live body earthworm is sieved to remove earthworm excrement, soil and the other impurities in earthworm；
（2）Cleaning：Live body earthworm after screening is put into service sink, soaks cleaning, to remove the silt on earthworm body
Impurity；
（3）Homogenate：The earthworm cleaned up is put into colloid mill uniformly to crush and wears into paste, earthworm slurries are made；
（4）Precipitate silt and filtering：Earthworm slurries are placed in lautertuns and adds in pure water and is filtered, earthworm slurries and pure water
Additional proportion is 1:1~1:2, lautertuns is divided into the first lautertuns and the second lautertuns, in the first lautertuns and the second lautertuns
Inlet is provided with intake valve, and the pure air after filtering is squeezed by intake valve in lautertuns, in the first lautertuns and
Screen pipe is respectively arranged in two lautertuns, is uniformly provided with filter opening on screen pipe, earthworm slurries are by the suction of pump from mistake
Filter opening extraction on chimney filter, so as to fulfill the separation of earthworm slurries and large granular impurity；Specifically filtration step is：First earthworm is starched
Liquid is passed through to the first lautertuns, and the intake valve that opening is arranged on the first lautertuns inlet is ventilated into the first lautertuns, with
The dispersibility of earthworm slurries is improved, convenient for salt precipitation protein below, intake valve is then shut off, opens pump, pass through pump
By the earthworm slurries in the first lautertuns, the intake line out of pond is filtered suction, and the aperture of screen pipe is in the first lautertuns
5mm, while gauze is provided on screen pipe, the aperture of gauze is 90 mesh, to increase filter effect, is made miscellaneous in earthworm slurries
Matter is removed more thorough, to improve the purity of earthworm protein, is then passed to further filtering and impurity removing in the second lautertuns, the second mistake
The aperture of filter pocket interior conduit is 2mm, while the gauze aperture being arranged on the second lautertuns pipeline is 120 mesh, through the second filtering
Earthworm slurries after slot filtering wait for subsequent processing through pumped；Screen pipe in first lautertuns and the second lautertuns is using more
Pipeline sets to increase filter area and improve filter efficiency；
（5）Stored frozen：Earthworm slurries after filtering are put into storage barrel, are stored under the conditions of -15 DEG C ~ -20 DEG C, it is spare；
（6）It thaws：The earthworm of stored frozen is taken out and is thawed under the conditions of 4 DEG C；
Earthworm liquid separation and Extraction step specifically includes：
（1）Prepare liquid of saltouing for the first time：The ammonium sulfate precipitation liquid that saturation degree is 30% is prepared, specially：It is pre-chilled at 100 parts 4 degree
17.6 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added in pure water, makes solid ammonium sulfate fully molten
Solution obtains ammonium sulfate precipitation liquid；
（2）It saltouts for the first time：The ammonium sulfate precipitation liquid that the saturation degree prepared is taken to be 30% is slowly added into earthworm slurries, and
Constantly earthworm slurries are stirred during the addition process, after ammonium sulfate precipitation liquid has added in, ammonium sulfate precipitation will be added in
The earthworm slurries of liquid are stood overnight, so that the protein in earthworm slurries precipitates completely, earthworm slurries and ammonium sulfate precipitation liquid
Additional proportion is 1:5；
（3）It centrifuges for the first time：Earthworm slurries after saltouing overnight centrifuge 15min under the rotating speed of 14000r/min, and respectively
First time centrifugation and first time centrifuged supernatant are collected, first time centrifugation is stored under the conditions of -20 DEG C, to first
Secondary centrifuged supernatant carries out pickle change；
（4）Preparation is saltoutd liquid：The ammonium sulfate precipitation liquid that saturation degree is 20% is prepared, specially：In the pure water of 4 degree of precoolings at 100 parts
13.4 parts of solid ammonium sulfate, and continuous stirring during the addition process are slowly added to, solid ammonium sulfate is made fully to dissolve to obtain
Ammonium sulfate precipitation liquid；
（5）Pickle change：Above-mentioned first time centrifuged supernatant under the conditions of 4 DEG C is pre-chilled, after precooling, takes the saturation prepared
The ammonium sulfate precipitation liquid spent for 20% is slowly added into first time centrifuged supernatant, and ammonium sulfate precipitation liquid with centrifuging for the first time
The adding proportion of clear liquid is 5:1, and constantly first time centrifuged supernatant is stirred in ammonium sulfate precipitation liquid adition process
It mixes, ammonium sulfate precipitations liquid is waited all to add in first time centrifuged supernatant, 1h is persistently stirred to first time centrifuged supernatant, it is quiet
It puts overnight；
（6）Second of centrifugation：Above-mentioned pickle change liquid is centrifuged into 15min, and collect second under the rotating speed of 14000r/min
Secondary centrifugation and second of centrifuged supernatant, second centrifuged supernatant is for fermentation fertilizer, second of centrifugation and the
Centrifugation set, obtains raw protein；
Raw protein is obtained into earthworm protein powder after enzymolysis step, ultrafiltration step again；
The preparation process of selenium-enriched earthworm albumen powder specifically includes：Selenium-rich rice powder, earthworm are added in the earthworm protein powder prepared
Earthworm albumen powder and the addition mass ratio of selenium-rich rice powder are 5:1, earthworm protein powder is mixed with selenium-rich rice powder with mixing device
It closes uniformly, obtains selenium-enriched earthworm albumen powder.
2. the preparation method of selenium-enriched earthworm albumen powder as described in claim 1, it is characterised in that：The enzymolysis of the earthworm protein
Step specifically includes：The raw protein for being obtained first time centrifugation and second of centrifugation set using food-grade NaOH
PH to 8 ~ 9 is adjusted, the complex enzyme of 40PPm ~ 100PPm is then added in into raw protein, the temperature of raw protein is made to be maintained at 40
DEG C ~ 50 DEG C at enzyme digestion reaction 10h ~ 14h, to remove the sugar and fat in raw protein.
3. the preparation method of selenium-enriched earthworm albumen powder as described in claim 1, it is characterised in that：The ultrafiltration of the earthworm protein
Step specifically includes：（1）Ceramic membrane filter：Deionized water is added in raw protein after enzymolysis, and by deionized water and slightly
Product albumen is uniformly mixing to obtain raw protein liquid, then is squeezed into raw protein liquid in ceramic membranous system by charging pump, ceramic membrane
Circulating pump in system is constantly run, and raw protein liquid is made to be filtered in ceramic membranous system, and ceramic membrane filter aperture is 50nm, with
Large granular impurity in raw protein liquid is removed, obtains raw protein penetrating fluid, the crossflow velocity control of ceramic membrane 4m/s ~
6m/s, cycle pump pressure control is in 0.2Mpa ~ 0.35Mpa；
（2）Ultrafiltration membrane concentrates：Raw protein penetrating fluid obtained above is squeezed into hyperfiltration membrane assembly, crude product after high-pressure pump is pressurized
Water in albumen penetrating fluid enters per-meate side through ultrafiltration membrane under high pressure, and the earthworm protein liquid in raw protein penetrating fluid is with height
Pressure flow is gone out, the molecular weight of ultrafiltration membrane retention for 5000 dalton, so as to reach in raw protein penetrating fluid earthworm protein and
The purpose of water separation, obtains earthworm protein concentrate；
（3）Freeze-drying：Above-mentioned earthworm protein concentrate is freeze-dried, it is earthworm protein powder to obtain powder product.
4. the preparation method of selenium-enriched earthworm albumen powder as claimed in claim 2, it is characterised in that：Wherein answering in enzymolysis step
Synthase includes alpha-galactosidase and lipase, and the carbohydrate in earthworm protein and fats can be degraded.
5. the preparation method of selenium-enriched earthworm albumen powder as described in claim 1, it is characterised in that：In selenium-enriched earthworm albumen powder
The mixing device used in preparation process includes mixing machine, and mixing machine is horizontally disposed on the ground, distinguishes at the both ends of mixing machine
It is provided with inlet port and outlet port, the inclined closing in shape of inlet port and outlet port, the bores of inlet port and outlet port is from mixing machine
Cylinder is gradually reduced outward, mixing machine lower end both sides is provided with driving device, mixing machine rolls under the drive of driving device
It is dynamic, the earthworm protein powder in mixing machine and selenium-rich rice powder are uniformly mixed, several baffles are provided in mixing machine, is kept off
The height of plate is less than the radius of cylinder, and micropore is uniformly provided on baffle, and mixing machine cylinder is divided into several solely by baffle
Vertical activity space, so as to which baffle forms barrier force to earthworm protein powder and selenium-rich rice powder when mixing machine rolls, in baffle
Blocking under earthworm protein powder and selenium-rich rice powder scatter, a part of earthworm protein powder and selenium-rich rice powder pass through on baffle
Micropore is fallen, and another part earthworm protein powder and selenium-rich rice powder are turned back under the barrier force of baffle and fallen, so as to reach earthworm
The purpose that albumen powder and selenium-rich rice powder are uniformly mixed.
6. the preparation method of selenium-enriched earthworm albumen powder as claimed in claim 5, it is characterised in that：The baffle includes four,
It is respectively and vertically fixed at the middle part in four orientation in upper and lower, left and right of mixing machine cylinder, the baffle is rectangle, the baffle
Length it is identical with the length of mixing machine cylinder, the width of the baffle is the 1/2 of the mixing machine cylinder radius, in the gear
The supporting rib of vertical interlaced is provided in the plane of plate, is sent out in mixing process due to the impact of albumen powder to prevent baffle plate
Raw deformed damaged.
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| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
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